CN113925169A - Gout relieving probiotic composition, and preparation method and application thereof - Google Patents
Gout relieving probiotic composition, and preparation method and application thereof Download PDFInfo
- Publication number
- CN113925169A CN113925169A CN202111392085.9A CN202111392085A CN113925169A CN 113925169 A CN113925169 A CN 113925169A CN 202111392085 A CN202111392085 A CN 202111392085A CN 113925169 A CN113925169 A CN 113925169A
- Authority
- CN
- China
- Prior art keywords
- gout
- powder
- lactobacillus
- probiotic composition
- relieving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 99
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 99
- 201000005569 Gout Diseases 0.000 title claims abstract description 98
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 89
- 239000000203 mixture Substances 0.000 title claims abstract description 82
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 94
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 claims abstract description 53
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 claims abstract description 53
- 229940116269 uric acid Drugs 0.000 claims abstract description 53
- 239000000463 material Substances 0.000 claims abstract description 22
- 239000000419 plant extract Substances 0.000 claims abstract description 13
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 10
- 239000008158 vegetable oil Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 3
- 239000000284 extract Substances 0.000 claims description 65
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 26
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 26
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 26
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 26
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 26
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 23
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 23
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 23
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 21
- 241000186606 Lactobacillus gasseri Species 0.000 claims description 20
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims description 19
- 229940009289 bifidobacterium lactis Drugs 0.000 claims description 19
- 235000007542 Cichorium intybus Nutrition 0.000 claims description 16
- 244000197580 Poria cocos Species 0.000 claims description 15
- 235000008599 Poria cocos Nutrition 0.000 claims description 15
- 241000234435 Lilium Species 0.000 claims description 14
- 240000008042 Zea mays Species 0.000 claims description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 14
- 235000005822 corn Nutrition 0.000 claims description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 13
- 241000186660 Lactobacillus Species 0.000 claims description 11
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims description 11
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims description 11
- 229940039696 lactobacillus Drugs 0.000 claims description 11
- 229920001202 Inulin Polymers 0.000 claims description 10
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 10
- 229940029339 inulin Drugs 0.000 claims description 10
- 239000000832 lactitol Substances 0.000 claims description 10
- VQHSOMBJVWLPSR-JVCRWLNRSA-N lactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-JVCRWLNRSA-N 0.000 claims description 10
- 235000010448 lactitol Nutrition 0.000 claims description 10
- 229960003451 lactitol Drugs 0.000 claims description 10
- 108010010803 Gelatin Proteins 0.000 claims description 9
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 9
- 229940107187 fructooligosaccharide Drugs 0.000 claims description 9
- 229920000159 gelatin Polymers 0.000 claims description 9
- 239000008273 gelatin Substances 0.000 claims description 9
- 235000019322 gelatine Nutrition 0.000 claims description 9
- 235000011852 gelatine desserts Nutrition 0.000 claims description 9
- 239000003549 soybean oil Substances 0.000 claims description 8
- 235000012424 soybean oil Nutrition 0.000 claims description 8
- 235000019483 Peanut oil Nutrition 0.000 claims description 7
- 235000010418 carrageenan Nutrition 0.000 claims description 7
- 239000000679 carrageenan Substances 0.000 claims description 7
- 229920001525 carrageenan Polymers 0.000 claims description 7
- 229940113118 carrageenan Drugs 0.000 claims description 7
- 239000000312 peanut oil Substances 0.000 claims description 7
- 235000010987 pectin Nutrition 0.000 claims description 7
- 229920001277 pectin Polymers 0.000 claims description 7
- 239000001814 pectin Substances 0.000 claims description 7
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims description 7
- 235000005687 corn oil Nutrition 0.000 claims description 6
- 239000002285 corn oil Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 229920002148 Gellan gum Polymers 0.000 claims description 5
- 235000010492 gellan gum Nutrition 0.000 claims description 5
- 239000000216 gellan gum Substances 0.000 claims description 5
- 235000011187 glycerol Nutrition 0.000 claims description 5
- 230000000116 mitigating effect Effects 0.000 claims description 5
- 239000004006 olive oil Substances 0.000 claims description 5
- 235000008390 olive oil Nutrition 0.000 claims description 5
- 235000010493 xanthan gum Nutrition 0.000 claims description 5
- 239000000230 xanthan gum Substances 0.000 claims description 5
- 229920001285 xanthan gum Polymers 0.000 claims description 5
- 229940082509 xanthan gum Drugs 0.000 claims description 5
- 235000010489 acacia gum Nutrition 0.000 claims description 4
- 229920000084 Gum arabic Polymers 0.000 claims description 3
- 239000000205 acacia gum Substances 0.000 claims description 3
- 244000215068 Acacia senegal Species 0.000 claims description 2
- 235000019486 Sunflower oil Nutrition 0.000 claims description 2
- 239000002600 sunflower oil Substances 0.000 claims description 2
- 244000298479 Cichorium intybus Species 0.000 claims 1
- 244000111489 Gardenia augusta Species 0.000 claims 1
- 239000004480 active ingredient Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 6
- 230000006872 improvement Effects 0.000 abstract description 2
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 19
- 241000723343 Cichorium Species 0.000 description 15
- 241000157835 Gardenia Species 0.000 description 14
- 208000024891 symptom Diseases 0.000 description 14
- 238000000034 method Methods 0.000 description 13
- 235000013336 milk Nutrition 0.000 description 11
- 239000008267 milk Substances 0.000 description 11
- 210000004080 milk Anatomy 0.000 description 11
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 10
- 241000700159 Rattus Species 0.000 description 10
- 235000005911 diet Nutrition 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 235000012054 meals Nutrition 0.000 description 9
- 239000003814 drug Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 230000037213 diet Effects 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 238000003304 gavage Methods 0.000 description 6
- 241000186000 Bifidobacterium Species 0.000 description 5
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 5
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 5
- 241000699670 Mus sp. Species 0.000 description 5
- 235000013405 beer Nutrition 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000000378 dietary effect Effects 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- 235000014102 seafood Nutrition 0.000 description 4
- 235000020238 sunflower seed Nutrition 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000036651 mood Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000186016 Bifidobacterium bifidum Species 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 201000001431 Hyperuricemia Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 101001076687 Lactobacillus gasseri Inulosucrase Proteins 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 206010003246 arthritis Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 229940002008 bifidobacterium bifidum Drugs 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000015816 nutrient absorption Nutrition 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000003371 toe Anatomy 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- RYYCJUAHISIHTL-UHFFFAOYSA-N 5-azaorotic acid Chemical compound OC(=O)C1=NC(=O)NC(=O)N1 RYYCJUAHISIHTL-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 239000004381 Choline salt Substances 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010017577 Gait disturbance Diseases 0.000 description 1
- IBFYXTRXDNAPMM-BVTMAQQCSA-N Geniposide Chemical compound O([C@@H]1OC=C([C@@H]2[C@H]1C(=CC2)CO)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O IBFYXTRXDNAPMM-BVTMAQQCSA-N 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 235000001188 Peltandra virginica Nutrition 0.000 description 1
- 241001619461 Poria <basidiomycete fungus> Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000026816 acute arthritis Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229960003235 allopurinol sodium Drugs 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000003833 bile salt Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 229960001338 colchicine Drugs 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 230000001882 diuretic effect Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000006750 hematuria Diseases 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 201000006334 interstitial nephritis Diseases 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 208000030175 lameness Diseases 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- MRWXACSTFXYYMV-FDDDBJFASA-N nebularine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC=C2N=C1 MRWXACSTFXYYMV-FDDDBJFASA-N 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229950000193 oteracil Drugs 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000010773 plant oil Substances 0.000 description 1
- BITYAPCSNKJESK-UHFFFAOYSA-N potassiosodium Chemical compound [Na].[K] BITYAPCSNKJESK-UHFFFAOYSA-N 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 208000016839 purine metabolism disease Diseases 0.000 description 1
- 239000002212 purine nucleoside Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- PTJRZVJXXNYNLN-UHFFFAOYSA-M sodium;2h-pyrazolo[3,4-d]pyrimidin-1-id-4-one Chemical compound [Na+].[O-]C1=NC=NC2=C1C=NN2 PTJRZVJXXNYNLN-UHFFFAOYSA-M 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/145—Gasseri
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a probiotic composition for relieving gout and a preparation method thereof, wherein probiotic freeze-dried powder, a plant extract, an auxiliary material and vegetable oil are uniformly mixed and are embedded by edible gum to prepare the probiotic composition; the invention also discloses application of the gout relieving probiotic composition in reducing uric acid and relieving gout. The invention realizes the effects of reducing the content of uric acid in vivo and relieving gout by reasonably proportioning the raw materials and ensuring the improvement of flora in intestinal tracts of human bodies. The invention is suitable for preparing the probiotic composition for relieving gout, and the prepared product is used for reducing uric acid and relieving gout.
Description
Technical Field
The invention belongs to the technical field of food, and relates to probiotics, in particular to a gout relieving probiotic composition, and a preparation method and application thereof.
Background
With the continuous improvement of living standard and dietary structure of people, multiple diseases such as hyperglycemia, hypertension, hyperlipidemia and hyperuricemia come with the people, wherein the incidence of uric acid is gradually increased and the people tend to be younger; uric acid is a final product of purine nucleoside metabolism, purine metabolism disorder causes gout, and the gout is mainly clinically manifested as hyperuricemia, acute arthritis, tophus, interstitial nephritis and the like, and the diseases bring physical pain to people and seriously affect the life quality of people.
Imbalance of intestinal flora can affect nutrient absorption of a host, so that problems of immune barrier, metabolic regulation and the like are caused, and probiotics are important flora in human intestinal tract and mainly have the effects of regulating the flora balance of the intestinal tract, improving nutrient absorption and enhancing the immunity of an organism; the strong absorption capacity of probiotics on purine, purine metabolic intermediate inosine, guanosine, and uric acid has been demonstrated; thus, the probiotic bacteria can decompose purine ingested from food in the intestinal tract, thereby reducing the absorption of purine by the human body and further reducing the blood uric acid level of the human body.
Chicory belongs to medicinal and edible herbaceous plants, and the chicory is described in Xinjiang Chinese herbal medicine: the modern medical research shows that the chicory extract can effectively reduce the uric acid concentration in human bodies and has long action duration; the corn stigma is sweet in taste and mild in nature, is an alkaline traditional Chinese medicinal material, has the effects of adjusting acid-base balance and promoting urination, and can promote uric acid in a human body to be discharged, so that the effect of treating gout can be achieved; the poria cocos has the advantages of mild nature, sweet and light taste, and has the effects of detoxifying, dehumidifying, joint benefiting and the like, and researches show that the poria cocos can promote urine discharge and can promote joints, so that the poria cocos has the effect of treating gout and reducing uric acid; the lily has the effects of tranquilizing and allaying excitement, is rich in nutritional ingredients such as protein, calcium, iron, magnesium, potassium and the like, contains multiple vitamins and a special component for treating gout, namely colchicine, can inhibit leucocyte dissimilation and alkalize urine, is beneficial to relieving gout arthritis, has the diuretic effect and can promote the excretion of uric acid; the gardenia has the functions of clearing heat, promoting diuresis, cooling blood and removing toxicity, and the jasminoidin effective components contained in the gardenia can promote the excretion of uric acid, thereby reducing the uric acid level in serum.
Disclosure of Invention
The invention aims to provide a probiotic composition for relieving gout, which can promote intestinal health, and simultaneously realize the purposes of reducing the content of uric acid and relieving gout;
the invention also aims to provide a preparation method of the gout relieving probiotic composition;
the invention also aims to provide application of the gout relieving probiotic composition.
In order to achieve the purpose, the invention adopts the technical scheme that:
a probiotic composition for relieving gout comprises probiotic lyophilized powder, plant extract, adjuvants, vegetable oil and edible gum.
As a limitation, the probiotic freeze-dried powder comprises, by weight, 1-3 parts of lactobacillus plantarum powder, 1-3 parts of bifidobacterium lactis powder, 1-3 parts of lactobacillus rhamnosus powder, 2-7 parts of lactobacillus acidophilus powder, 1-10 parts of lactobacillus reuteri powder and 1-10 parts of lactobacillus griffithii powder.
As a further limitation, the content of the lactobacillus plantarum in the lactobacillus plantarum powder is 8 x 1010～8× 1011cfu/g；
The content of Bifidobacterium lactis in the Bifidobacterium lactis powder is 3 × 1010～4×1011cfu/g；
The content of Lactobacillus rhamnosus in the Lactobacillus rhamnosus powder is 8 × 1010～4×1011cfu/g；
The content of Lactobacillus acidophilus in the Lactobacillus acidophilus powder is 8 × 109～3×1010cfu/g；
The content of Lactobacillus reuteri in the Lactobacillus reuteri powder is 8 × 109～2×1010cfu/g；
The Lactobacillus gasseri powder contains Grignard milkThe bacillus content is 3 × 109～2×1010cfu/g。
As another limitation, the plant extract comprises 1-50 parts by weight of gardenia extract, 1-30 parts by weight of lily extract, 5-50 parts by weight of tuckahoe extract, 1-40 parts by weight of corn stigma extract and 10-35 parts by weight of chicory extract.
As a third limitation, the adjuvant is at least one of fructooligosaccharide, lactitol, xylooligosaccharide, inulin, and isomaltooligosaccharide; the dosage of the auxiliary materials is 10-50 parts by weight.
As a fourth definition, the vegetable oil is at least one of soybean oil, sunflower oil, olive oil, corn oil, and peanut oil;
the dosage of the vegetable oil is 3-5 times of the total weight of the probiotic freeze-dried powder, the plant extract and the auxiliary materials.
As a fifth limitation, the edible gum is at least one of gelatin, pectin, carrageenan, gellan gum, glycerin, gum arabic, and xanthan gum;
the dosage of the edible gum is 10-30% of the total weight of the vegetable oil, the probiotic freeze-dried powder, the plant extract and the auxiliary materials.
The invention also provides a preparation method of the gout relieving probiotic composition, which comprises the steps of uniformly mixing the probiotic freeze-dried powder, the plant extract, the auxiliary material and the plant oil, and embedding the mixture by using edible gum to obtain the gout relieving probiotic composition.
The invention also provides application of the probiotic composition for relieving gout in reducing uric acid and relieving gout.
Due to the adoption of the technical scheme, compared with the prior art, the invention has the technical progress that:
the probiotic composition for relieving gout is a dietary therapy probiotic compound, has no side effect, selects the gardenia extract, the lily extract, the poria cocos extract, the corn stigma extract and the chicory plant extract in homology of medicine and food, and is beneficial to reducing the concentration of uric acid, reducing the generation of uric acid, promoting blood circulation, dredging channels and relieving gout symptoms;
the gout relieving probiotic composition provided by the invention is rich in probiotics content, has six different probiotics, is huge in active probiotics quantity and different in function, and can be used for synergistically conditioning intestinal tracts, regulating metabolism and reducing purine absorption and hematuria level;
the prebiotics, namely inulin and isomaltooligosaccharide, are used as auxiliary materials, so that the proliferation of probiotics can be promoted, harmful bacteria can be inhibited, and the absorption utilization rate of the gout relieving probiotic composition is improved;
the preparation method of the probiotic composition for relieving gout provided by the invention adopts colloid for embedding, so that the influence of adverse factors such as gastric acid, choline and bile salt on probiotics can be effectively reduced, the survival rate of the probiotics reaching the intestinal tract is improved, and the number of intestinal probiotic groups is increased.
The invention is suitable for preparing the probiotic composition, and the prepared probiotic composition can be used for reducing uric acid and relieving gout.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
FIG. 1 is the serum uric acid content of a mouse in example 8 of the present invention;
FIG. 2 is a summary report seal proof chart of clinical experiments according to the present invention;
FIG. 3 is a cross-section seal proof chart of the summary report of clinical experiments according to the present invention.
Detailed Description
The present invention is further illustrated by the following specific examples, which are to be construed as merely illustrative, and not limitative of the remainder of the disclosure.
Example 1 preparation method of a gout-relieving probiotic composition
The embodiment comprises the following steps which are carried out in sequence:
s1, pretreatment:
taking Lactobacillus plantarum with content of 6 multiplied by 10112kg of cfu/g lactobacillus plantarum powder and 4 multiplied by 10 bifidobacterium lactis content111.5kg of cfu/g milk bifidobacterium bifidum powder with the content of lactobacillus rhamnosus of 4 multiplied by 1011cfu/g Lactobacillus rhamnosus powder 1.5kg, Lactobacillus acidophilus content 3 × 10105kg of cfu/g lactobacillus acidophilus powder and 2 multiplied by 10 of lactobacillus reuteri content106kg of cfu/g lactobacillus reuteri powder with the content of lactobacillus gasseri of 2 multiplied by 10109kg of lactobacillus gasseri powder per gram, 50kg of gardenia extract, 20kg of lily extract, 10kg of poria cocos extract, 20kg of corn stigma extract, 10kg of chicory extract, 5kg of fructo-oligosaccharide, 10kg of lactitol, 5kg of xylo-oligosaccharide, 5kg of inulin and 25kg of isomalto-oligosaccharide, and uniformly mixing to obtain a dry material A;
s2, mixing materials:
adding 600kg of soybean oil into the mixed material A, and uniformly stirring to obtain a wet material B;
s2, embedding:
and (4) embedding the wet material B by using 80kg of gelatin to obtain the gout relieving probiotic composition X1.
Examples 2-6 methods of preparing gout-relieving probiotic compositions
Examples 2 to 6 are processes for preparing a gout-alleviating probiotic composition, which are substantially the same as in example 1, except that the raw materials are used in different amounts, as detailed in table 1:
TABLE 1 summary of the parameters of examples 2-6
Examples 2-6 other parts of the disclosure, same as example 1, examples 2-6 were prepared into gout-relieving probiotic compositions X2-X6, respectively.
Example 7 preparation of a control gout mitigation probiotic composition
This example prepares two control gout mitigation probiotic compositions, including the following steps performed in sequence:
s1, respectively taking 15kg of lactitol, 1kg of xylo-oligosaccharide, 20kg of inulin, 10kg of isomalto-oligosaccharide and 2 multiplied by 10 of lactobacillus plantarum112kg of cfu/g lactobacillus plantarum powder and 4 multiplied by 10 bifidobacterium lactis content111.5kg of cfu/g milk bifidobacterium bifidum powder with the content of lactobacillus rhamnosus of 4 multiplied by 1011cfu/g Lactobacillus rhamnosus powder 1.5kg, Lactobacillus acidophilus content 8 × 10105kg of cfu/g lactobacillus acidophilus powder and 2 multiplied by 10 of lactobacillus reuteri content106kg of cfu/g lactobacillus reuteri powder with the content of lactobacillus gasseri of 3 multiplied by 10109kg of cfu/g lactobacillus gasseri powder, uniformly mixing, adding 213kg of soybean oil, uniformly stirring to obtain a wet material alpha, and embedding the wet material alpha by 30kg of gelatin to obtain the gout relieving probiotic composition Y1;
s2, respectively taking 15kg of lactitol, 1kg of xylo-oligosaccharide, 20kg of inulin, 10kg of isomaltooligosaccharide, 9kg of poria extract, 5kg of corn stigma extract, 20kg of gardenia extract, 5kg of lily extract and 10kg of chicory extract, uniformly mixing, adding 285kg of soybean oil, uniformly stirring to obtain wet material beta, and embedding the wet material beta by using 35kg of gelatin to obtain the gout relieving probiotic composition Y2.
Example 8 animal experiments with gout relief probiotic compositions
The embodiment is an animal experiment of the gout relieving probiotic composition, and the specific steps are as follows:
experimental animals: selecting pure-breed healthy SD male rats with 3-4 weeks of age and weight of 60 +/-10 g;
the experimental scheme is as follows: selecting 30 pure-breed healthy SD male rats with 3-4 weeks of age, after adapting to feeding for 7 days, randomly dividing the SD male rats into a normal group, a model group, a comparison group, an experiment 1 group, an experiment 2 group and an experiment 3 group, feeding high-purine colonized rat grains to the model group, the comparison group, the experiment 1 group, the experiment 2 group and the experiment 3 group from the 8 th day to the end of an animal experiment, injecting 1ml of oteracil potassium-sodium carboxymethylcellulose suspension into the abdominal cavity according to the weight standard, and providing common rat grains to the normal group; all rats had free access to water;
beginning on day 15, the model group is subjected to intragastric administration of 1ml of physiological saline according to intragastric administration standards; a contrast group, performing intragastric allopurinol-sodium carboxymethylcellulose suspension treatment on each rat according to intragastric standard; experiment 1 group, each rat was treated by gavage Y1 according to gavage criteria; experiment 2 group, each rat was treated by gavage Y2 according to gavage standard; experiment 3 group, each rat was treated with gavage X2 according to gavage standard; blood is collected from the tail vein at regular time every day during the treatment process for blood uric acid detection.
The serum uric acid content of the six groups of mice was measured, and the results are shown in table 2 and fig. 1:
TABLE 2 serum uric acid levels in mice at different time points
As can be seen from Table 2, the groups 1 and 2 can reduce the content of uric acid in serum of mice, and the content of uric acid in serum can be remarkably reduced in 7 days; compared with the experiment 1 and the experiment 2, the experiment 3 can obviously reduce the content of serum uric acid of the mouse; the result shows that the single probiotics or the plant extract can reduce the content of uric acid in mice, the effect of reducing serum uric acid of the mice is more obvious when the two are combined, the gout relieving probiotic composition X2 has longer drug effect time and less uric acid reduction when being used as food compared with a certain medicine sold in the market, but the gout relieving probiotic composition X2 can relieve gout symptoms when being used as food, and has no toxic or side effect.
Example 9 efficacy validation of gout relief probiotic compositions
The embodiment is an efficacy verification experiment of a gout relieving probiotic composition, and the specific steps are as follows:
the efficacy of the gout-relieving probiotic composition X1-X6 prepared in examples 1-6 was further tested for gout patients with high uric acid content, gout symptoms and dietary contraindication;
the taking method comprises the following steps: the gout relieving probiotic composition is taken 3 g each time, 2 times in 1 day, and is taken with water in half an hour after meals, the effect is observed after taking for 15-30 days, and the result is shown in table 3:
TABLE 3
As can be seen from table 3, after the gout patients take the gout relieving probiotic composition prepared by the invention, the uric acid level is reduced, the gout symptoms are relieved, the diet is not limited any more, and a proper amount of seafood, hot pot and sugar-containing beverage can be eaten.
Gout-relieving probiotic composition
Summary report of clinical trial
Summary of the experiments report
Case 1: male; before taking, the toes are edema, difficult to walk and low in emotion, and the uric acid value is 617 umol/L;
taking the product: a gout-relieving probiotic composition X1;
probiotic composition X1 comprising Lactobacillus plantarum 6 × 101120g of cfu/g lactobacillus plantarum powder and 4 multiplied by 10 bifidobacterium lactis content11cfu/g milk bifidobacterium powder 15g, rhamnose lactobacillus content 4X 1011cfu/g lactobacillus rhamnosus powder 15g, Lactobacillus acidophilus content 3 × 1010cfu/g Lactobacillus acidophilus powder 50g, Lactobacillus reuteri content 2 × 1010cfu/g of lactobacillus reuteri powder 60g, the content of lactobacillus gasseri is 2 multiplied by 101090g of cfu/g of lactobacillus gasseri powder, 500g of gardenia extract, 200g of lily extract, 100g of poria cocos extract, 200g of corn stigma extract, 100g of chicory extract, 50g of fructo-oligosaccharide, 100g of lactitol, 50g of xylo-oligosaccharide, 50g of inulin, 250g of isomalto-oligosaccharide and 6000g of soybean oilUniformly prepared by embedding 800g of gelatin;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: after taking the medicine for 15 days, the uric acid value is reduced to 402 umol/L; the beer is comfortable, no pain is caused when 200ml of beer is drunk, the edema of toes disappears, walking is normal, and the mood is pleasant.
Case 2: male; eating the mutton soup to cause gout attack, lameness and lean on the crutch, wherein the uric acid value is 578 umol/L;
taking the product: a gout-relieving probiotic composition X2;
probiotic composition X2 containing Lactobacillus plantarum 8 × 1010cfu/g plant lactobacillus powder 25g, bifidobacterium lactis content 1 x 1011cfu/g bifidobacterium lactis powder 20g, lactobacillus rhamnosus content 1 x 1011cfu/g lactobacillus rhamnosus powder 15g, Lactobacillus acidophilus content 8 × 109cfu/g Lactobacillus acidophilus powder 70g, Lactobacillus reuteri content 8.5 × 10980g of cfu/g lactobacillus reuteri powder and 8 multiplied by 10 of lactobacillus gasseri content850g of cfu/g of lactobacillus gasseri powder, 10g of gardenia extract, 250g of lily extract, 500g of poria cocos extract, 300g of corn stigma extract, 150g of chicory extract, 100g of fructo-oligosaccharide, 2000g of sunflower seed oil and 2710g of peanut oil, and the mixture is prepared by embedding 300g of pectin and 328g of carrageenan;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: the uric acid level is reduced to 469umol/L after the taking of the drug for 15 days; diarrhea appeared on day 4, the patient recovered to normal on day 6, walking was normal on day 10 without turning, and gout attack was not seen after eating a little shrimp meat 15 days later.
Case 3: male; the gout symptom is obvious, the joint is gout, the uric acid is high, and the uric acid value is 592 umol/L;
taking the product: a gout-relieving probiotic composition X3;
probiotic composition X3 containing Lactobacillus plantarum 8 × 101110g of cfu/g lactobacillus plantarum powder and 3 multiplied by 10 bifidobacterium lactis content10cfu/g bifidobacterium lactis powder 30g, lactobacillus rhamnosus content 2 x 1011cfu/g of lactobacillus rhamnosus powder 30g, lactobacillus acidophilus content 1 × 1010cfu/g Lactobacillus acidophilus powder 20g, and Lactobacillus reuteri content 9 × 10910g of cfu/g lactobacillus reuteri powder and 5 multiplied by 10 of lactobacillus gasseri content890g of cfu/g of lactobacillus gasseri powder, 400g of gardenia extract, 300g of lily extract, 350g of poria cocos extract, 250g of corn stigma extract, 250g of chicory extract, 100g of lactitol, 200g of xylo-oligosaccharide, 1000g of soybean oil, 1500g of sunflower seed oil, 1500g of olive oil, 1640g of corn oil and 1500g of peanut oil, and the mixture is prepared by embedding 300g of gelatin, 250g of pectin, 400g of carrageenan, 150g of gellan gum, 650g of xanthan gum, 400g of glycerol and 604g of arabic gum;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: the uric acid level is reduced to 513umol/L after the taking day 15; constipation appears on day 5, the health is recovered after two days, the constipation is smooth and free of foreign odor, the gout symptom is relieved after half a month, and the gout symptom does not appear due to the fact that a small amount of beer is paid for eating some seafood.
Case 4: male; the uric acid is higher, gout often appears when the diet is improper, and the uric acid value is 660 umol/L;
taking the product: a gout-relieving probiotic composition X4;
probiotic composition X4 containing Lactobacillus plantarum 1 × 1011cfu/g lactobacillus plantarum powder 30g, bifidobacterium lactis content 8 x 1010cfu/g milk bifidobacterium powder 10g, rhamnose lactobacillus content 3 x 1011cfu/g Lactobacillus rhamnosus powder 25g, Lactobacillus acidophilus content 9 × 109cfu/g Lactobacillus acidophilus powder 30g, and Lactobacillus reuteri 1 × 1010cfu/g lactobacillus reuteri powder 100g, content of lactobacillus gasseriIs 6 x 10830g of cfu/g of lactobacillus gasseri powder, 350g of gardenia extract, 10g of lily extract, 50g of poria cocos extract, 10g of corn stigma extract, 200g of chicory extract, 100g of xylo-oligosaccharide, 50g of inulin, 50g of isomaltooligosaccharide, 2500g of corn oil and 2202.5g of peanut oil, and the mixture is prepared by embedding 500g of gellan gum, 200g of xanthan gum and 448.5g of glycerol;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: after taking the medicine for 15 days, the uric acid value is reduced to 541 umol/L; when a bowl of soybean milk is drunk on the 6 th day, gout symptoms do not appear, and after the soybean milk is drunk on the 15 th day, no gout is induced by eating the soybean milk boiled in Beijing brine.
Case 5: male; high uric acid, preference for carbonated beverage, occasional gout, and uric acid value of 537 umol/L;
taking the product: a gout-relieving probiotic composition X5;
probiotic composition X5 comprising Lactobacillus plantarum at a content of 5 × 101115g of cfu/g lactobacillus plantarum powder and 3 multiplied by 10 bifidobacterium lactis content11cfu/g milk bifidobacterium powder 25g, rhamnose lactobacillus content 8 x 1010cfu/g Lactobacillus rhamnosus powder 20g, Lactobacillus acidophilus content is 2 × 1010cfu/g Lactobacillus acidophilus powder 60g, and Lactobacillus reuteri content 1.5 × 101020g of cfu/g lactobacillus reuteri powder and 7 multiplied by 10 of lactobacillus gasseri content8100g of cfu/g of lactobacillus gasseri powder, 50g of gardenia extract, 90g of lily extract, 150g of poria cocos extract, 400g of corn stigma extract, 350g of chicory extract, 30g of fructo-oligosaccharide, 30g of lactitol, 30g of xylo-oligosaccharide, 30g of inulin, 30g of isomalto-oligosaccharide, 2000g of olive oil, 2000g of corn oil and 3150g of peanut oil are uniformly mixed, and the mixture is prepared by embedding 200g of gelatin, 200g of pectin, 100g of carrageenan and 400g of acacia gum;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: the uric acid level is reduced to 469umol/L after the taking of the drug for 15 days; during the 30-day taking process, the food and drink are not controlled, and gout symptoms do not appear.
Case 6: male; gout symptom is obvious when eating seafood-limping, diet is forbidden seriously at ordinary times, life quality is affected, mood is low, and uric acid value is 564 umol/L;
taking the product: a gout-relieving probiotic composition X6;
probiotic composition X6 containing Lactobacillus plantarum 3 × 101120g of cfu/g lactobacillus plantarum powder and 2 multiplied by 10 bifidobacterium lactis content11cfu/g milk bifidobacterium powder 15g, rhamnose lactobacillus content 3.5X 1011cfu/g Lactobacillus rhamnosus powder 10g, Lactobacillus acidophilus content 3 × 101040g of cfu/g lactobacillus acidophilus powder and 8 multiplied by 10 of lactobacillus reuteri content940g of cfu/g lactobacillus reuteri powder and 3 multiplied by 10 of lactobacillus gasseri content970g of cfu/g of lactobacillus gasseri powder, 200g of gardenia extract, 170g of lily extract, 250g of poria cocos extract, 400g of corn stigma extract, 350g of chicory extract, 50g of fructo-oligosaccharide, 100g of lactitol, 50g of xylo-oligosaccharide, 50g of isomaltose hypgather, 1500g of soybean oil, 1000g of sunflower seed oil, 2000g of olive oil and 1560g of corn oil, and the preparation method is prepared by embedding 200g of pectin, 150g of carrageenan, 350g of gellan gum, 150g of xanthan gum and 150g of glycerol;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: after taking for 15 days, the uric acid value is reduced to 424 umol/L; after taking for 15 days, the seafood, the broth and the hot pot can be eaten in a small amount, gout symptoms are not generated, the life quality is improved, and the mood is good.
Case 7: male; after eating chafing dish, mutton soup and soybean milk, the phalanges generate gout symptoms, and the uric acid value is 404 umol/L;
taking the product: a gout-relieving probiotic composition X1;
probiotic composition X1, comprising Lactobacillus plantarum6×101120g of cfu/g lactobacillus plantarum powder and 4 multiplied by 10 bifidobacterium lactis content11cfu/g milk bifidobacterium powder 15g, rhamnose lactobacillus content 4X 1011cfu/g lactobacillus rhamnosus powder 15g, Lactobacillus acidophilus content 3 × 1010cfu/g Lactobacillus acidophilus powder 50g, Lactobacillus reuteri content 2 × 1010cfu/g of lactobacillus reuteri powder 60g, the content of lactobacillus gasseri is 2 multiplied by 101090g of cfu/g of lactobacillus gasseri powder, 500g of gardenia extract, 200g of lily extract, 100g of poria cocos extract, 200g of corn stigma extract, 100g of chicory extract, 50g of fructo-oligosaccharide, 100g of lactitol, 50g of xylo-oligosaccharide, 50g of inulin, 250g of isomalto-oligosaccharide and 6000g of soybean oil are uniformly mixed and embedded by 800g of gelatin to prepare the compound;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: after taking the medicine for 15 days, the uric acid value is reduced to 400 umol/L; after taking the medicine for 30 days, dietetic restraint is not needed any more, and gout symptoms do not appear.
Case 8: male; the beer is more remunerative, the beer is loved to drink, the uric acid is high, the diet is not restricted, and the uric acid value is 493 umol/L;
taking the product: a gout-relieving probiotic composition X2;
probiotic composition X2 containing Lactobacillus plantarum 8 × 1010cfu/g plant lactobacillus powder 25g, bifidobacterium lactis content 1 x 1011cfu/g bifidobacterium lactis powder 20g, lactobacillus rhamnosus content 1 x 1011cfu/g lactobacillus rhamnosus powder 15g, Lactobacillus acidophilus content 8 × 109cfu/g Lactobacillus acidophilus powder 70g, Lactobacillus reuteri content 8.5 × 10980g of cfu/g lactobacillus reuteri powder and 8 multiplied by 10 of lactobacillus gasseri content850g of cfu/g of lactobacillus gasseri powder, 10g of gardenia extract, 250g of lily extract, 500g of poria cocos extract, 300g of corn stigma extract, 150g of chicory extract, 100g of fructo-oligosaccharide, 2000g of sunflower seed oil and 2710g of peanut oil, and the mixture is prepared by embedding 300g of pectin and 328g of carrageenan;
the taking method comprises the following steps: 3 g of the gout relieving probiotic composition is taken orally every time, and is taken twice a day;
the taking time is as follows: the oral liquid is taken with warm water half an hour after meals;
the effect is as follows: the uric acid level is reduced to 436umol/L after the administration day 15; after 30 days of administration, the diet was not moderated and uric acid was reduced.
According to the case information, after the gout patients take the probiotic composition prepared by the invention, the uric acid value is reduced, the gout symptoms are relieved, the diet is not limited any more, and the seafood, the hot pot and the sugar-containing beverage can be eaten in a proper amount.
Claims (9)
1. The probiotic composition for relieving gout is characterized in that raw materials for preparing active ingredients of the probiotic composition comprise probiotic freeze-dried powder, plant extracts, auxiliary materials, vegetable oil and edible gum.
2. The probiotic composition for relieving gout as claimed in claim 1, wherein the probiotic freeze-dried powder comprises, by weight, 1-3 parts of lactobacillus plantarum powder, 1-3 parts of bifidobacterium lactis powder, 1-3 parts of lactobacillus rhamnosus powder, 2-7 parts of lactobacillus acidophilus powder, 1-10 parts of lactobacillus reuteri powder and 1-10 parts of lactobacillus grignard powder.
3. The gout mitigation probiotic composition of claim 2, wherein:
the content of Lactobacillus plantarum in the Lactobacillus plantarum powder is 8 multiplied by 1010～8×1011cfu/g；
The content of Bifidobacterium lactis in the Bifidobacterium lactis powder is 3 × 1010～4×1011cfu/g；
The content of Lactobacillus rhamnosus in the Lactobacillus rhamnosus powder is 8 × 1010～4×1011cfu/g；
The content of lactobacillus acidophilus in the lactobacillus acidophilus powder is8×109～3×1010cfu/g；
The content of Lactobacillus reuteri in the Lactobacillus reuteri powder is 8 × 109～2×1010cfu/g；
The content of Lactobacillus gasseri in the Lactobacillus gasseri powder is 3 multiplied by 109～2×1010cfu/g。
4. The gout-relieving probiotic composition as claimed in claim 1, wherein the plant extract comprises, by weight, 1 to 50 parts of gardenia extract, 1 to 30 parts of lily extract, 5 to 50 parts of poria cocos extract, 1 to 40 parts of corn stigma extract and 10 to 35 parts of chicory extract.
5. The gout-alleviating probiotic composition according to claim 1, wherein the auxiliary material is at least one of fructo-oligosaccharide, lactitol, xylo-oligosaccharide, inulin and isomalto-oligosaccharide;
the dosage of the auxiliary materials is 10-50 parts by weight.
6. The gout mitigation probiotic composition of claim 1, wherein the vegetable oil is at least one of soybean oil, sunflower oil, olive oil, corn oil, and peanut oil;
the dosage of the vegetable oil is 3-5 times of the total weight of the probiotic freeze-dried powder, the plant extract and the auxiliary materials.
7. The probiotic composition for relieving gout as claimed in claim 1, wherein the edible gum is at least one of gelatin, pectin, carrageenan, gellan gum, glycerin, gum arabic and xanthan gum;
the dosage of the edible gum is 10-30% of the total weight of the vegetable oil, the probiotic freeze-dried powder, the plant extract and the auxiliary materials.
8. The preparation method of the gout-relieving probiotic composition as claimed in any one of claims 1 to 7, wherein the preparation method comprises the steps of uniformly mixing the probiotic freeze-dried powder, the plant extract, the auxiliary material and the vegetable oil, and embedding the mixture with edible gum to obtain the gout-relieving probiotic composition.
9. Use of the gout mitigation probiotic composition of any one of claims 1 to 7 for reducing uric acid and relieving gout.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111392085.9A CN113925169A (en) | 2021-11-23 | 2021-11-23 | Gout relieving probiotic composition, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111392085.9A CN113925169A (en) | 2021-11-23 | 2021-11-23 | Gout relieving probiotic composition, and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113925169A true CN113925169A (en) | 2022-01-14 |
Family
ID=79287372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111392085.9A Pending CN113925169A (en) | 2021-11-23 | 2021-11-23 | Gout relieving probiotic composition, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113925169A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114432346A (en) * | 2022-03-24 | 2022-05-06 | 金华银河生物科技有限公司 | Composite probiotic composition for treating hyperuricemia and gout and preparation method and application thereof |
| CN118320052A (en) * | 2024-04-12 | 2024-07-12 | 久睦本(海南)科技股份有限公司 | Composition for gout conditioning, preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103652891A (en) * | 2013-11-28 | 2014-03-26 | 浙江省农业科学院 | Lactobacillus casei embedding microcapsule and preparation method thereof |
| CN108159291A (en) * | 2017-12-07 | 2018-06-15 | 韩大春 | Plant extracts and probiotics compounding product and preparation method thereof |
| CN110623182A (en) * | 2019-10-10 | 2019-12-31 | 胡洁 | Probiotic plant solid beverage for treating hyperuricemia and gout |
| CN113288978A (en) * | 2021-07-14 | 2021-08-24 | 李智民 | Composition for treating gout and preparation method and application thereof |
-
2021
- 2021-11-23 CN CN202111392085.9A patent/CN113925169A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103652891A (en) * | 2013-11-28 | 2014-03-26 | 浙江省农业科学院 | Lactobacillus casei embedding microcapsule and preparation method thereof |
| CN108159291A (en) * | 2017-12-07 | 2018-06-15 | 韩大春 | Plant extracts and probiotics compounding product and preparation method thereof |
| CN110623182A (en) * | 2019-10-10 | 2019-12-31 | 胡洁 | Probiotic plant solid beverage for treating hyperuricemia and gout |
| CN113288978A (en) * | 2021-07-14 | 2021-08-24 | 李智民 | Composition for treating gout and preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114432346A (en) * | 2022-03-24 | 2022-05-06 | 金华银河生物科技有限公司 | Composite probiotic composition for treating hyperuricemia and gout and preparation method and application thereof |
| CN118320052A (en) * | 2024-04-12 | 2024-07-12 | 久睦本(海南)科技股份有限公司 | Composition for gout conditioning, preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104855956A (en) | Medical formula food for insomnia | |
| CN104187709A (en) | Total nutrient formula food suitable for patients having obesity or fat-reducing operation | |
| CN105029407A (en) | Medical formula food for inflammatory bowel disease | |
| CN104187611A (en) | Total nutrient formula food for gastrointestinal malabsorption | |
| CN105394763A (en) | Pregnant-woman diabetes total-nutrient formula food | |
| CN105410896A (en) | Hepatogenic diabetes full nutritional formula food | |
| CN104855985A (en) | Non-complete-nutrition formula food for high uric acid | |
| CN105533705A (en) | Medical formula food for recuperating women during second trimester | |
| CN104187632A (en) | Total-nutrient formulated food eaten by patients with osteoporosis | |
| CN104839661A (en) | High uric acid formula food | |
| CN113925169A (en) | Gout relieving probiotic composition, and preparation method and application thereof | |
| CN104256642A (en) | Fully nutrient formula food for lowering high blood pressure, high blood sugar and high blood lipid and strengthening yang | |
| CN104171798A (en) | Full-nutrition formula food suitable for patients with trauma, infection, operation and other stress states | |
| CN104839684A (en) | Medical formula food for people with helicobacter pylori related gastritis | |
| CN104839678A (en) | Medical formula food for people with nephrosis | |
| CN105029393A (en) | Medical formula food for children | |
| CN104839656A (en) | Liver disease medicine formula food | |
| CN104187651A (en) | High-uric-acid prevention total-nutrition formula food | |
| CN104187613A (en) | Total-nutrient formulated food for patients with insomnia | |
| CN104187641A (en) | Total nutrient formula food for phlegm-dampness constitution | |
| CN105326038A (en) | Pregnant-woman pregnancy-vomiting total-nutrient formula food | |
| CN105380263A (en) | Full-nutrition formula food for treating pregnant woman constipation | |
| CN104839655A (en) | High blood lipids, blood sugar and blood pressure lowering and yang tonifying medical formula food | |
| CN104996992A (en) | Medical formula food for muscle attenuation syndromes | |
| CN104839672A (en) | Medical formula food for people with constitution of qi stagnation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |