CN104623122B - A kind of biological agent and preparation method thereof with anti-fatigue effect - Google Patents

A kind of biological agent and preparation method thereof with anti-fatigue effect Download PDF

Info

Publication number
CN104623122B
CN104623122B CN201510014572.XA CN201510014572A CN104623122B CN 104623122 B CN104623122 B CN 104623122B CN 201510014572 A CN201510014572 A CN 201510014572A CN 104623122 B CN104623122 B CN 104623122B
Authority
CN
China
Prior art keywords
weight
parts
chinese medicine
microorganism
lactobacillus
Prior art date
Application number
CN201510014572.XA
Other languages
Chinese (zh)
Other versions
CN104623122A (en
Inventor
江瀚
陈琦冈
Original Assignee
江瀚生物科技(上海)有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 江瀚生物科技(上海)有限公司 filed Critical 江瀚生物科技(上海)有限公司
Priority to CN201510014572.XA priority Critical patent/CN104623122B/en
Publication of CN104623122A publication Critical patent/CN104623122A/en
Application granted granted Critical
Publication of CN104623122B publication Critical patent/CN104623122B/en

Links

Abstract

The present invention provides a kind of biological agent and preparation method thereof with anti-fatigue effect.The active constituent of a large amount of anti-fatigue abilities is rich in biological agent product prepared by the present invention, such as: flavonoids (4.43%), soaping agents (82.4mg/100g), amino acid (4.21%), glutathione (886mg/100g), vitamin C (322mg/100g), vitamin E (908 μ g/100g), SOD (194000U/100g), lactate (5.04%) and various trace elements (iron: 503mg/kg;Selenium: 0.18mg/kg etc.) etc., and has antifatigue function, it is converted by microorganism composite fermentation technology centering the effective elements of the medicine, it makes it have and is easier to be absorbed and utilized, the features such as significant effect is a kind of to natural biological preparation that is anti-oxidant, removing free radical, improve human-body fatigue situation.

Description

A kind of biological agent and preparation method thereof with anti-fatigue effect
Technical field
The present invention relates to a kind of biological agents more particularly to a kind of with the biological agent of anti-fatigue effect and its production side Method.
Background technique
Fatigue refers to that organism physiology process cannot continue on the specified level of its function and/or cannot maintain scheduled movement Intensity.It is one and is related to the comprehensive physiology course of many Physiology and biochemistry factors, is that human body is mental or physical exertion is to one Determine a kind of normal physiological phenomenon necessarily occurred when the stage, it had not only indicated the temporary decline of the original ability to work of body, but also It may be the tendency that body develops to sick and wounded state.Fatigue can make working efficiency reduction, slow in reacting, study to things Efficiency decline, prolonged fatigue, which can generate overwork, to be caused to go to bits, except certain organs and system it is overstretched cause it is various Outside disease damage, it there is also the hypofunctions such as circulation, breathing, digestion function, the symptoms such as headache, dizziness, nausea, thirsty, out of strength occur.
It is so-called antifatigue, it is exactly the generation of delay fatigue and/or the elimination that accelerates fatigue.Can delay fatigue generation and/ Or the substance to accelerate elimination of fatigue claims anti-fatigue active substance.The common substance for having anti-fatigue effect have ginseng, American Ginseng, Octacosanol, taurine, pueraria lobata, pilose antler and jujube etc..China, which has developed thousands of kinds, at present has anti-fatigue effect Health food, as CN102948748A discloses a kind of antifatigue health care product.According to main composition and health-care effect, It is broadly divided into supplement energy, supplement vitamin and microelement needed by human and by improving functions of biological organs acceleration bodies The three classes such as intracellular metabolite product discharge.
Existing patent of invention is generally developed with Chinese herbal medicine or its extract, such as CN103446423A discloses one Kind Anti-fatigue healthcare traditional Chinese medicine.Chinese herbal medicine is needed by processing or processing, and there is remarkable result in the side of taking after effective component extracting;If It is directly taken without processing, then due to the obstacle of absorption, effective component is not easy to utilize, and effect is unobvious.
Summary of the invention
The object of the present invention is to provide it is a kind of can it is anti-oxidant, remove free radical, improve human-body fatigue situation natural biological Preparation.
To achieve the above object, technical solution of the present invention provides a kind of complex microorganism hair for having anti-fatigue effect The natural biological preparation of ferment Chinese medicine production.
The first aspect of the invention is to provide a kind of microorganism formulation with anti-fatigue effect, the complex microorganism Strain includes the parts by weight of following component and each component:
10-20 parts of saccharomyces cerevisiae;
20-30 parts of lactobacillus acidophilus;
20-30 parts of lactobacillus plantarum;
20-30 parts of Bifidobacterium;
Wherein, the saccharomyces cerevisiae, lactobacillus acidophilus, lactobacillus plantarum, Bifidobacterium pass through cultivation and fermentation;
The parts by weight of the Chinese medicine material, brown sugar and water:
40-65 parts of Chinese medicine material;
2-5 parts of brown sugar;
40-60 parts of water.
Wherein, the complex microorganism bacterial classification and the weight ratio of the Chinese medicine material, brown sugar and water are 1:15-20.
Wherein, the Chinese medicine material includes at least pueraria lobata, sea-buckthorn and grape pip.
It wherein, further include at least one of antierythrite, lactose, protective agent in the biological agent.
The production method that the second aspect of the invention is to provide a kind of above-mentioned biological agent: step includes:
Step 1: complex microorganism bacterial classification preparation
(1.1), saccharomyces cerevisiae is accessed in potato fluid nutrient medium and is cultivated to culture medium muddiness;By lactobacillus acidophilus, It is muddy to culture medium that lactobacillus plantarum, Bifidobacterium are respectively connected to culture in MRS fluid nutrient medium;
(1.2), it by above-mentioned strain after cultivation, is prepared by following weight percent: saccharomyces cerevisiae 10-20 parts by weight, Lactobacillus acidophilus 20-30 parts by weight, lactobacillus plantarum 20-30 parts by weight, Bifidobacterium 20-30 parts by weight;
(1.3), the ratio that (1.2) weighed strain is 1:80-120 by weight is put into raw material I and is fermented, obtained compound Microorganism fungus kind;
Wherein, the raw material I includes brown sugar, water;
Step 2: the compound microorganism ferments liquid preparation of Chinese medicine
By complex microorganism bacterial classification obtained by step 1 and raw material II in 1:15-20 ratio culture;
Wherein, the raw material II includes Chinese medicine material, brown sugar, water;
Step 3: the compound microorganism ferments concentrate preparation of Chinese medicine
(3.1), the compound microorganism ferments liquid of Chinese medicine obtained by step 2 is added into water, after making solid-to-liquid ratio 1:1, successively soaked Bubble, elution;
(3.2), leacheate is concentrated, obtains the compound microorganism ferments concentrate of Chinese medicine;
Step 4: microorganism formulation preparation
Step 3 is obtained into the complex microorganism concentrate and at least one of antierythrite, lactose, protective agent of Chinese medicine Microorganism formulation described in solid or liquid is made in mixing.
Wherein, in (1.1) of the step 1 saccharomyces cerevisiae condition of culture are as follows: under conditions of 28-32 DEG C, 180- 220rpm shaken cultivation 48-72 hours;Lactobacillus acidophilus, lactobacillus plantarum, the condition of culture of Bifidobacterium are equal are as follows: in 35-39 Under the conditions of DEG C, cultivate 48-72 hours.
Wherein, the raw material I is by weight ratio are as follows: brown sugar 5-10%, water 90-98%;The raw material II is by weight ratio Are as follows: Chinese medicine material 40-65 parts by weight, brown sugar 2-5 parts by weight, water 40-60 parts by weight;Wherein, the Chinese medicine material be selected from pueraria lobata, Sea-buckthorn and grape pip etc..
Wherein, the condition of culture of (1.3) of the step 1 be 28-32 DEG C, it is closed culture, to pH be less than 3.60-4.00 Afterwards, it cultivates 12-15 days.
Wherein, the Chinese medicine material in the raw material II is powdered, and partial size is 30-50 mesh.
Wherein, the condition of culture of the step 2 is cultivated 12-15 days under the conditions of 28-32 DEG C;Step 3 obtains compound micro- Need to be under the conditions of 60-70 DEG C, after sterilizing 10-30min after bioconcentration liquid, 2-4 DEG C of storage is stand-by.
A kind of currently preferred embodiment provides a kind of solid particle preparation preparation method, provides in parts by weight former Material, wherein
Specific steps are as follows:
Antierythrite and lactose are first sequentially added into spray drying one-step-granulating method, then start to spray into composite natral product Concentrate flow 10ml/ minutes, finally sprays into the protective agent being dissolved in suitable quantity of water, 60 DEG C of the inlet air temperature of granulator, outlet air Temperature 45 C, discharge when temperature drops to 30 DEG C in day with fog to be painted to get.
A kind of currently preferred embodiment provides a kind of preparation method of liquid oral medicine, provides in parts by weight former Material, wherein
45-50 parts of composite natral product concentrate;
45-50 parts of antierythrite;
5-10 parts of protective agent;
Specific steps are as follows:
Appropriate hot distilled water stirring and dissolving first is added in antierythrite and protective agent, is added after being cooled to 25-35 DEG C compound Natural products concentrate stirs after clarifying solution to obtain the final product.
Wherein, the strain is bought by Chinese agriculture Microbiological Culture Collection administrative center
Saccharomyces cerevisiae (Saccharomyces cerevisiae, ACCC20065)
Lactobacillus acidophilus (Lactobacillus acidophilus, ACCC10637)
Lactobacillus plantarum (Lactobacillus plantarum, ACCC11118)
Bifidobacterium (Bifidobacterium, ACCC11054)
Wherein, the solid pharmaceutical preparation include granule, pulvis etc. any one or a few.
Wherein, the auxiliary agent includes sugar and protective agent.
Wherein, the sugar is any one in glucose, sucrose, fructose, lactose, antierythrite, threitol, xylitol Kind is several.
Wherein, the protective agent in maltodextrin, cornstarch, Sucralose, citric acid any one or it is several Kind.
The utility model has the advantages that the present invention defends the strain in " the strain list that can be used for food " of planning commission's bulletin using country, adopt It is raw material with common Chinese medicine and health care product, carries out natural biological preparation made of composite fermentation.The present invention combines existing guarantor The effective component of the first kind and the second class in strong product is rich in the active constituent of a large amount of anti-fatigue abilities in product, such as: flavones Class (4.43%), soaping agents (82.4mg/100g), amino acid (4.21%), glutathione (886mg/100g), vitamin C (322mg/100g), vitamin E (908 μ g/100g), SOD (194000U/100g), lactate (5.04%) and lot of trace Element (iron: 503mg/kg;Selenium: 0.18mg/kg etc.) etc..
Biological agent prepared by the present invention has antifatigue function, has by microorganism composite fermentation technology to Chinese medicine Effect ingredient is converted, and is made it have and is easier to be absorbed and utilized, the features such as significant effect, be it is a kind of to it is anti-oxidant, remove free radical, Improve the natural biological preparation of human-body fatigue situation.
Specific embodiment
With reference to embodiment, the present invention is described in further detail.
Embodiment one (solid dosage forms)
The first step, the production production of hybrid seeds
Saccharomyces cerevisiae (the Saccharomyces that will be bought from Chinese agriculture Microbiological Culture Collection administrative center Cerevisiae, ACCC20065) potato fluid nutrient medium of transferring conical flask in, at 28 DEG C, 180-220rpm oscillation training It supports 48 hours, is taken out after culture medium is muddy;Lactobacillus acidophilus (Lactobacillus acidophilus, ACCC10637), Lactobacillus plantarum (Lactobacillus plantarum, ACCC11118), Bifidobacterium (Bifidobacterium, ACCC11054), transfer in the conical flask of MRS fluid nutrient medium, cultivated 48 hours at 37 DEG C respectively, after culture medium is muddy It takes out, is then placed in 4 DEG C of refrigerators and saves for use;
Second step, strain compound criteria
(1) strain that step 1 is cultivated is weighed by following weight percent: saccharomyces cerevisiae 15%, lactobacillus acidophilus 30%, Lactobacillus plantarum 30%, Bifidobacterium 25%;
(2) strain compound criteria: the above weighed strain is put into the raw material of following weight proportion in 1:100 ratio: Brown sugar 5%, pure water 95% are put into fermentor 30 DEG C of closed cultures after mixing evenly, cultivate 15 days after pH is less than 3.60;
Third step, raw material composite fermentation
(1) raw material crushes: screening individual uniform pueraria lobata, sea-buckthorn, grape pip being passed through pulverizer respectively and are ground into 40 mesh;
(2) composite fermentation: the strain of the above compound criteria is put into the raw material of following proportion in 1:20 ratio: pueraria lobata 25%, sea-buckthorn 20%, grape pip 5%, brown sugar 5%, pure water 45% are put into fermentor 30 DEG C of closed cultures 15 after mixing evenly It;
4th step, concentrate preparation
(1) water is added in fermentor, solid-to-liquid ratio is made to reach 1:1, is impregnated 1 day, is then eluted;
(2) leacheate carries out being concentrated into 20% at 50 DEG C;
(3) it is stand-by to sterilize 4 DEG C of storages after twenty minutes at 60 DEG C for the composite natral product liquid after being concentrated.
5th step, solid dosage preparation
Material is weighed by following weight ratio: composite natral product concentrate 20%, antierythrite 20%, lactose 55%, Protective agent 5%, first sequentially adds spray drying one-step-granulating method for antierythrite and lactose, then starts to spray into composite natral production Object concentrate, flow 10ml/ minutes, the protective agent that finally penetrating is dissolved in suitable quantity of water, 60 DEG C of the inlet air temperature of granulator, out 45 DEG C of air temperature, discharge when temperature drops to 30 DEG C in day with fog to be painted to get.
Embodiment two (oral solution type)
Step 1: with embodiment one;
Step 2: strain compound criteria
(1) strain that step 1 is cultivated is weighed by following weight percent: saccharomyces cerevisiae 10%, lactobacillus acidophilus 30%, Lactobacillus plantarum 30%, Bifidobacterium 30%;
(2) strain compound criteria: the above weighed strain is put into the raw material of following proportion in 1:100 ratio: brown sugar 3%, pure water 97% is put into fermentor 32 DEG C of closed cultures after mixing evenly, cultivates 12 days after pH is less than 3.60;
Third step, raw material composite fermentation
(1) raw material crushes: screening individual uniform pueraria lobata, sea-buckthorn, grape pip being passed through pulverizer respectively and are ground into 40 mesh;
(2) composite fermentation: the strain of the above compound criteria is put into the raw material of following proportion in 1:15 ratio: pueraria lobata 30%, sea-buckthorn 20%, grape pip 5%, brown sugar 5%, pure water 40% are put into fermentor 32 DEG C of closed cultures 12 after mixing evenly It;
4th step, concentrate preparation
(1) water is added in fermentor, solid-to-liquid ratio is made to reach 1:1, is impregnated 1 day, is then eluted.
(2) leacheate carries out being concentrated into 20% at 45 DEG C
(3) it is stand-by to sterilize 4 DEG C of storages after ten minutes at 65 DEG C for the composite natral product liquid after being concentrated;
5th step, the preparation of oral solution type
Material is weighed by following weight ratio: composite natral product concentrate 45%, antierythrite 45%, protective agent 5- 10%, appropriate hot distilled water stirring and dissolving first is added in antierythrite and protective agent, composite natral is added after being cooled to 35 DEG C and produces Object concentrate stirs after clarifying solution to obtain the final product.
Embodiment three:
According to " health food is examined and assessment technique specification (2003 editions) ", the inspection evaluated according to function of physical fatigue alleviation Proved recipe method carries out.
Animal packet: the healthy cleaning grade male mice in kunming of selection, weight 18-22g, after conventional adaptation is fed 2 days, It is randomly divided into 2 groups (blank group, administration groups).
The determination of dosage: according to the small of people's recommended dose (1.5g solid dosage forms/d 50ml liquid dosage form/d) The given low that mouse equivalent dose is used as, i.e. 0.30g solid dosage forms/kg BW/d or 10ml liquid dosage form/kg BW/d, orally Administration, stomach-filling volume 20mL/kgBW, blank group stomach-filling distilled water.
Experimental method:
It is administered once daily, successive administration 14 days.After last dose 30min, the 5% weight galvanized wire of load in mouse tail root It is placed in swimming trunk went swimming.The depth of water is no less than 30cm, 25 ± 1.0 DEG C of water temperature, record mouse from swimming to the time of death. Judgment criteria, i.e. mouse head submerged are exhausted referring to the MCARLE power recommended, the water surface can't be returned through 10s is considered as power and exhaust. Record from swim time for being exhausted to power as swimming time.Data are with mean and standard deviationIt indicates.Each group number According to homogeneity test of variance is carried out, variance is neat, and group difference is examined using t.Test result is shown in Table 1:
The 1 each group mouse exhausted swimming time of table compares
Group Number of animals (only) Dosage Walking weight load (s)
Blank group 10 - 158±54
Liquid dosage form group 10 10ml/kg 280±67**
Solid dosage forms group 10 0.30g/kg 294±108**
Note: compared to the blank group,**P<0.01。
From above-mentioned data it is known that the preparation prepared by the method for the present invention, each administration group can highly significant prolong It is good anti-to therefore deduce that the method for the present invention prepares having for antifatigue biological agent for long walking weight load (P < 0.01) Fatigue effect.
Specific embodiments of the present invention are described in detail above, but it is merely an example, the present invention is simultaneously unlimited It is formed on particular embodiments described above.To those skilled in the art, any couple of present invention carries out equivalent modifications and Substitution is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by equal transformation and Modification, all should be contained within the scope of the invention.

Claims (8)

1. a kind of application of complex microorganism preparations in the drug or health care product that preparation has anti-fatigue effect, feature exist In the microorganism formulation ferments through following raw materials according: complex microorganism bacterial classification, Chinese medicine material, brown sugar and water;
Wherein, the complex microorganism bacterial classification includes following component: saccharomyces cerevisiae (Saccharomyces cerevisiae), thermophilic Lactobacillus lactis (Lactobacillus acidophilus), lactobacillus plantarum (Lactobacillus acidophilus) and double Discrimination bacillus (Lactobacillus acidophilus);
Wherein, the Chinese medicine material is pueraria lobata, sea-buckthorn and grape pip.
2. application according to claim 1, which is characterized in that the complex microorganism bacterial classification includes following component and each group The parts by weight divided:
Saccharomyces cerevisiae 10-20 parts by weight;
Lactobacillus acidophilus 20-30 parts by weight;
Lactobacillus plantarum 20-30 parts by weight;
Bifidobacterium 20-30 parts by weight;
Wherein, the saccharomyces cerevisiae, lactobacillus acidophilus, lactobacillus plantarum, Bifidobacterium pass through cultivation and fermentation.
3. application according to claim 1, which is characterized in that the parts by weight of the Chinese medicine material, brown sugar and water are as follows:
Chinese medicine material 40-65 parts by weight;
Brown sugar 2-5 parts by weight;
Water 40-60 parts by weight.
4. application according to claim 1, which is characterized in that described using the Chinese medicine material, brown sugar and water as raw material II The weight ratio of complex microorganism bacterial classification and the raw material II is 1:15-20.
5. application according to claim 1, which is characterized in that the microorganism formulation is solid pharmaceutical preparation or liquid port system of mourning Agent.
6. application according to claim 1, which is characterized in that further include in the microorganism formulation antierythrite, lactose, At least one of protective agent.
7. the production method of microorganism formulation that one kind is applied as described in claim 1, which is characterized in that step includes:
Step 1: complex microorganism bacterial classification preparation
(1.1), saccharomyces cerevisiae is accessed in potato fluid nutrient medium and is cultivated to culture medium muddiness;By lactobacillus acidophilus, plant It is muddy to culture medium that lactobacillus, Bifidobacterium are respectively connected to culture in MRS fluid nutrient medium;
(1.2), it by above-mentioned strain after cultivation, is prepared by following weight percent: saccharomyces cerevisiae 10-20 parts by weight, acidophilus Lactobacillus 20-30 parts by weight, lactobacillus plantarum 20-30 parts by weight, Bifidobacterium 20-30 parts by weight;
(1.3), the ratio that (1.2) weighed strain is 1:80-120 by weight is put into raw material I and is fermented, obtain composite microbial Object strain;
Wherein, the raw material I includes brown sugar, water;
Wherein, the fermentation condition is 28-32 °C, sealed fermenting, is less than under the conditions of 3.60-4.00 to pH;
Step 2: the compound microorganism ferments liquid preparation of Chinese medicine
By complex microorganism bacterial classification obtained by step 1 and raw material II in 1:15-20 ratio culture;
Wherein, the raw material II includes Chinese medicine material, brown sugar, water;
Wherein, Chinese medicine material is powdered, and partial size is 30-50 mesh;
Wherein, the condition of culture is cultivated 12-15 days under the conditions of 28-32 °C;
Step 3: the compound microorganism ferments concentrate preparation of Chinese medicine
(3.1), the compound microorganism ferments liquid of Chinese medicine obtained by step 2 is added into water, after making solid-to-liquid ratio 1:1, successively impregnates, drenches It washes;
(3.2), leacheate is concentrated, obtains the compound microorganism ferments concentrate of Chinese medicine;
Step 4: microorganism formulation preparation
The Chinese medicine compound microorganism ferments concentrate and at least one of antierythrite, lactose, protective agent that step 3 is obtained Microorganism formulation described in solid or liquid is made in mixing;
Wherein, obtain after complex microorganism concentrate need under the conditions of 60-70 °C, after sterilizing 10-30min, 2-4 °C of storage to With.
8. production method according to claim 7, which is characterized in that the training of saccharomyces cerevisiae in (1.1) of the step 1 The condition of supporting are as follows: under conditions of 28-32 °C, 180-220rpm shaken cultivation 48-72 hours;Lactobacillus acidophilus, lactobacillus plantarum, The condition of culture of Bifidobacterium is equal are as follows: under the conditions of 35-39 °C, cultivates 48-72 hours.
CN201510014572.XA 2015-01-12 2015-01-12 A kind of biological agent and preparation method thereof with anti-fatigue effect CN104623122B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510014572.XA CN104623122B (en) 2015-01-12 2015-01-12 A kind of biological agent and preparation method thereof with anti-fatigue effect

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510014572.XA CN104623122B (en) 2015-01-12 2015-01-12 A kind of biological agent and preparation method thereof with anti-fatigue effect

Publications (2)

Publication Number Publication Date
CN104623122A CN104623122A (en) 2015-05-20
CN104623122B true CN104623122B (en) 2019-02-22

Family

ID=53202684

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510014572.XA CN104623122B (en) 2015-01-12 2015-01-12 A kind of biological agent and preparation method thereof with anti-fatigue effect

Country Status (1)

Country Link
CN (1) CN104623122B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105106454A (en) * 2015-08-25 2015-12-02 上海普乐拜格健康科技有限公司 Natural biological preparation for improving irritable premature climacteric syndromes and method for preparing natural biological preparation
CN105125756A (en) * 2015-08-25 2015-12-09 上海普乐拜格健康科技有限公司 Natural biological preparation for improving quality of breast milk and preparation method of natural biological preparation
CN105211850A (en) * 2015-08-26 2016-01-06 江瀚生物科技(上海)有限公司 A kind of based on sea-buckthorn and the root of kudzu vine fermentation method and products thereof for antioxidant stoste
CN105112329A (en) * 2015-08-26 2015-12-02 上海普乐拜格健康科技有限公司 Method for preparing special microbial preparations for homemade 'fruit and vegetable enzymes', products of method and application of products
CN105614880A (en) * 2015-12-28 2016-06-01 上海普乐拜格健康科技有限公司 Biological agent for repairing irritable bowel mucosa damage and preparation method thereof
CN105535282A (en) * 2015-12-28 2016-05-04 上海普乐拜格健康科技有限公司 Biological agent for irritable infertility function repair and preparation method thereof
CN105497318A (en) * 2015-12-28 2016-04-20 上海普乐拜格健康科技有限公司 Biological agent used for enhancing immunity of organism and preparation method thereof
CN106399163B (en) * 2016-09-08 2019-09-27 江苏微康生物科技有限公司 A kind of anti-aging probiotics preparation and preparation method
CN108576807A (en) * 2018-04-04 2018-09-28 江瀚生物科技(上海)有限公司 A kind of small molecule nutrient and preparation method thereof effectively improving elderly population calcium absorption rate

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103156061A (en) * 2013-04-15 2013-06-19 山东德州神牛药业有限公司 Compound microbial active additive
CN104524024A (en) * 2014-12-15 2015-04-22 江瀚生物科技(上海)有限公司 Compound microorganism preparation for improving male sperm quality and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103156061A (en) * 2013-04-15 2013-06-19 山东德州神牛药业有限公司 Compound microbial active additive
CN104524024A (en) * 2014-12-15 2015-04-22 江瀚生物科技(上海)有限公司 Compound microorganism preparation for improving male sperm quality and preparation method thereof

Also Published As

Publication number Publication date
CN104623122A (en) 2015-05-20

Similar Documents

Publication Publication Date Title
CN104030845B (en) A kind of glossy ganoderma substituting stuff cultivation substratum that with the addition of Grosvenor Momordica
CN104509864B (en) A kind of have nutritional health food improving gastrointestinal function and preparation method thereof
CN105167072B (en) A kind of production method of feature matrimony vine ferment and its product
CN104138527B (en) A kind of biofermentation compositions and application thereof with antitumaous effect
CN103549215B (en) Intensive pig farm weanling pig feed and feeding mode thereof
CN103355666B (en) Preparation method of plant enzyme beverage capable of improving cardiovascular and cerebrovascular metabolic functions
CN102919565B (en) Meat chick granulated feed containing active bacterium fermentation ingredient and traditional Chinese medicine ingredient
CN105029603A (en) Composite fresh flower, fruit, vegetable and herb enzyme, and preparation method and application thereof
CN103704483B (en) A kind of feed utilizing Chinese medicine dreg to ferment and preparation method thereof
CN101172902B (en) Method for formulating culture medium additive agent for cultivation of selenium-rich ganoderma lucidum
CN1969657B (en) Probiotic feed additive used for pig
CN103549217B (en) Safe and efficient biological feed additive for pigs
CN100362091C (en) A selenium yeast product, a method of preparing a selenium yeast product and the use of the product for preparing food, a dietary supplement or a drug
CN105918821A (en) Compound enzyme liquid having effects of lowering high blood lipids, high blood pressure and high blood sugar, and improving immunity, and preparation method thereof
CN103652321B (en) A kind of biological feedstuff of stalk leavening, biological feedstuff of stalk and production method thereof
US20070172492A1 (en) Method for fermentation and cultivation, fermented plant extract, fermented plant extract powder, and composition containing the extract of fermented plant
CN104285820B (en) One strain selenium-enriched cordceps militaris strain and application thereof
CN104856015A (en) A cordyceps sinensis enzyme and a preparation method thereof
CN105559064A (en) Five-alga probiotic enzyme tablets and preparation method thereof
CN104826087B (en) Compound Moringa polypeptide and its preparation method and application
CN103299887B (en) Method for preparing phellinus igniarius hypha blocks by utilizing sprouted rice
CN101624570B (en) Method for cultivating cultured ganoderma lucidum with Chinese medicaments and method for preparing cultured ganoderma lucidum health-care product
CN102242044B (en) Cordyceps cicadae wine and preparation method thereof
CN103553834B (en) Method for preparing tremella cultivation material from tea cattail and tea seed shells
CN104119120B (en) Organic/inorganic compound fertilizer special for blueberry

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant