CN113017092A - Fermentation type small molecule peptide mixed solution for regulating blood sugar and preparation method and application thereof - Google Patents
Fermentation type small molecule peptide mixed solution for regulating blood sugar and preparation method and application thereof Download PDFInfo
- Publication number
- CN113017092A CN113017092A CN202110356523.XA CN202110356523A CN113017092A CN 113017092 A CN113017092 A CN 113017092A CN 202110356523 A CN202110356523 A CN 202110356523A CN 113017092 A CN113017092 A CN 113017092A
- Authority
- CN
- China
- Prior art keywords
- enzymolysis
- fermentation
- small molecule
- blood sugar
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000008280 blood Substances 0.000 title claims abstract description 54
- 210000004369 blood Anatomy 0.000 title claims abstract description 54
- 238000000855 fermentation Methods 0.000 title claims abstract description 49
- 230000004151 fermentation Effects 0.000 title claims abstract description 49
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 150000003384 small molecules Chemical class 0.000 title claims abstract description 24
- 239000011259 mixed solution Substances 0.000 title claims abstract description 21
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 235000008599 Poria cocos Nutrition 0.000 claims abstract description 25
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 15
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 15
- 108090000526 Papain Proteins 0.000 claims abstract description 15
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 15
- 239000004365 Protease Substances 0.000 claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 15
- 239000003814 drug Substances 0.000 claims abstract description 15
- 108010093305 exopolygalacturonase Proteins 0.000 claims abstract description 15
- 235000013305 food Nutrition 0.000 claims abstract description 15
- 235000008434 ginseng Nutrition 0.000 claims abstract description 15
- 229940055729 papain Drugs 0.000 claims abstract description 15
- 235000019834 papain Nutrition 0.000 claims abstract description 15
- 239000006041 probiotic Substances 0.000 claims abstract description 15
- 235000018291 probiotics Nutrition 0.000 claims abstract description 15
- 108010059892 Cellulase Proteins 0.000 claims abstract description 14
- 229940106157 cellulase Drugs 0.000 claims abstract description 14
- 240000000249 Morus alba Species 0.000 claims abstract description 10
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 10
- 241001523681 Dendrobium Species 0.000 claims abstract description 8
- 240000005717 Dioscorea alata Species 0.000 claims abstract description 4
- 235000004879 dioscorea Nutrition 0.000 claims abstract description 4
- 244000197580 Poria cocos Species 0.000 claims description 24
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 17
- 239000008103 glucose Substances 0.000 claims description 17
- 241000208340 Araliaceae Species 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 239000002131 composite material Substances 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 9
- 238000000108 ultra-filtration Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 230000000529 probiotic effect Effects 0.000 claims description 6
- 235000015468 Lycium chinense Nutrition 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 239000000825 pharmaceutical preparation Substances 0.000 claims description 5
- 229940127557 pharmaceutical product Drugs 0.000 claims description 5
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims description 4
- 241001608472 Bifidobacterium longum Species 0.000 claims description 4
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 4
- 229940009289 bifidobacterium lactis Drugs 0.000 claims description 4
- 229940009291 bifidobacterium longum Drugs 0.000 claims description 4
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 3
- 244000199866 Lactobacillus casei Species 0.000 claims description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 3
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 3
- 229940017800 lactobacillus casei Drugs 0.000 claims description 3
- 239000002552 dosage form Substances 0.000 claims description 2
- 244000241838 Lycium barbarum Species 0.000 claims 1
- 241000756042 Polygonatum Species 0.000 claims 1
- 239000008297 liquid dosage form Substances 0.000 claims 1
- 239000007909 solid dosage form Substances 0.000 claims 1
- 230000000975 bioactive effect Effects 0.000 abstract description 8
- 235000017784 Mespilus germanica Nutrition 0.000 abstract description 6
- 244000182216 Mimusops elengi Species 0.000 abstract description 6
- 235000000560 Mimusops elengi Nutrition 0.000 abstract description 6
- 235000007837 Vangueria infausta Nutrition 0.000 abstract description 6
- 235000001188 Peltandra virginica Nutrition 0.000 abstract description 5
- 240000006079 Schisandra chinensis Species 0.000 abstract description 5
- 235000008422 Schisandra chinensis Nutrition 0.000 abstract description 5
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 abstract description 5
- 229930003944 flavone Natural products 0.000 abstract description 5
- 150000002212 flavone derivatives Chemical class 0.000 abstract description 5
- 235000011949 flavones Nutrition 0.000 abstract description 5
- 229920001542 oligosaccharide Polymers 0.000 abstract description 5
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 5
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 5
- 230000002195 synergetic effect Effects 0.000 abstract description 5
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 abstract description 5
- 239000012530 fluid Substances 0.000 abstract description 4
- 230000000968 intestinal effect Effects 0.000 abstract description 4
- 229920001184 polypeptide Polymers 0.000 abstract description 4
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract description 4
- 229930182490 saponin Natural products 0.000 abstract description 4
- 150000007949 saponins Chemical class 0.000 abstract description 4
- 235000002723 Dioscorea alata Nutrition 0.000 abstract description 3
- 235000005362 Dioscorea floribunda Nutrition 0.000 abstract description 3
- 235000004868 Dioscorea macrostachya Nutrition 0.000 abstract description 3
- 235000005361 Dioscorea nummularia Nutrition 0.000 abstract description 3
- 235000005360 Dioscorea spiculiflora Nutrition 0.000 abstract description 3
- 230000017531 blood circulation Effects 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 235000007056 Dioscorea composita Nutrition 0.000 abstract description 2
- 235000009723 Dioscorea convolvulacea Nutrition 0.000 abstract description 2
- 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 abstract description 2
- 235000006533 astragalus Nutrition 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 241001061264 Astragalus Species 0.000 abstract 1
- 244000131316 Panax pseudoginseng Species 0.000 abstract 1
- 244000248825 Peltandra virginica Species 0.000 abstract 1
- 210000004233 talus Anatomy 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 18
- 230000000694 effects Effects 0.000 description 12
- 238000000465 moulding Methods 0.000 description 10
- 206010012601 diabetes mellitus Diseases 0.000 description 8
- 235000002722 Dioscorea batatas Nutrition 0.000 description 6
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 6
- 240000001811 Dioscorea oppositifolia Species 0.000 description 6
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 210000003734 kidney Anatomy 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 244000241872 Lycium chinense Species 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 4
- 238000003304 gavage Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 208000022531 anorexia Diseases 0.000 description 2
- 230000001914 calming effect Effects 0.000 description 2
- 208000026106 cerebrovascular disease Diseases 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 206010061428 decreased appetite Diseases 0.000 description 2
- 230000002526 effect on cardiovascular system Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007661 gastrointestinal function Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 229940126680 traditional chinese medicines Drugs 0.000 description 2
- 241000045403 Astragalus propinquus Species 0.000 description 1
- 241000186016 Bifidobacterium bifidum Species 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 240000004638 Dendrobium nobile Species 0.000 description 1
- 206010060891 General symptom Diseases 0.000 description 1
- 208000008454 Hyperhidrosis Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 206010023424 Kidney infection Diseases 0.000 description 1
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000030768 Optic nerve injury Diseases 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 206010037596 Pyelonephritis Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 206010061481 Renal injury Diseases 0.000 description 1
- 206010038776 Retching Diseases 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 208000031971 Yin Deficiency Diseases 0.000 description 1
- 210000004404 adrenal cortex Anatomy 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003130 blood coagulation factor inhibitor Substances 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000019788 craving Nutrition 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 206010013781 dry mouth Diseases 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 210000000750 endocrine system Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003414 extremity Anatomy 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000037315 hyperhidrosis Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 208000037806 kidney injury Diseases 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 230000006996 mental state Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 231100000862 numbness Toxicity 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 230000003393 splenic effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/513—Adolescentes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Diabetes (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Endocrinology (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Emergency Medicine (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Obesity (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a fermentation type small molecule peptide mixed solution for regulating blood sugar, a preparation method and application thereof, and relates to the technical field of small molecule peptide mixed solutions. The invention utilizes the common fermentation of tuckahoe bacterial liquid, compound probiotics, mulberry leaf, sealwort, dendrobium, schisandra, astragalus, ginseng, yam and medlar, the zymolysis of the zymotic fluid is carried out by cellulase, pectinase and papain, the fermentation fluid is ultrafiltered to obtain the fermentation type small molecular peptide mixed solution which is rich in a plurality of bioactive factors such as polypeptide, oligosaccharide, saponin, flavone and the like, and the various bioactive factors have synergistic effect. According to the invention, by selecting and proportioning various components in the fermentation liquor, the small molecular peptide mixed liquor capable of regulating intestinal flora, improving immunity and blood circulation is finally obtained, and the healthy level of blood sugar can be maintained on the whole. Therefore, the fermented small molecule peptide mixed solution can be used for preparing food or medicine for maintaining healthy blood sugar level.
Description
Technical Field
The invention belongs to the technical field of small molecule peptide mixed liquor, and particularly relates to fermented small molecule peptide mixed liquor for regulating blood sugar as well as a preparation method and application thereof.
Background
The glucose in the blood is called blood glucose. Glucose is an important constituent of the human body and also an important source of energy. The normal human body needs a lot of sugar every day to provide energy, and provides power for the normal operation of various tissues and organs. Blood glucose must be maintained at a certain level to maintain the needs of the organs and tissues in the body. The production and utilization of normal human blood glucose is in a state of dynamic equilibrium, maintained at a relatively stable level, due to the fact that the source and the way of blood glucose are approximately the same. Blood sugar level is very important in human life, diabetes is easily caused by overhigh blood sugar level, clinical manifestations are many (edema, limb numbness and the like), complications are many (optic nerve injury, kidney injury, infection and the like), and the damage to the kidney is also great. Therefore, it is very important to maintain healthy blood sugar level in the current social background.
Therefore, how to select raw materials with high efficiency, low cost, short preparation period, simple and clear technical route and easy control of market circulation quality to process foods or medicines so as to achieve the effect of maintaining the healthy blood sugar level of human bodies becomes a main problem in the technical field of traditional Chinese medicine component extraction and preparation at present.
Disclosure of Invention
In view of the above, the present invention provides a fermented small molecule peptide mixed solution for regulating blood sugar, and a preparation method and an application thereof, wherein the preparation method can greatly improve the utilization rate of medicinal and edible raw materials, reduce the cost, greatly increase the bioavailability and the biological activity of blood sugar, and perform synergistic effect on various bioactive factors in the fermented small molecule peptide mixed solution, so as to regulate the organism in multiple directions, and improve the whole body, thereby achieving an optimal maintenance mode of blood sugar level.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of a fermentation type small molecule peptide mixed solution for regulating blood sugar, which comprises the following steps: (1) mixing Poria bacteria liquid and composite probiotic with Ginseng radix, rhizoma Dioscoreae, rhizoma Polygonati, fructus Lycii, herba Dendrobii and folium Mori, and fermenting together to obtain fermentation liquid; the fermentation temperature is 26-28 ℃, the humidity is 62-70%, and the time is 16-24 h; the strains of the composite probiotics comprise: bifidobacterium adolescentis, Bifidobacterium lactis, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus rhamnosus;
(2) sequentially carrying out enzymolysis on the fermentation liquor by using cellulase, pectinase and papain to obtain enzymolysis liquid;
(3) and intercepting components with the molecular weight of less than 2000Da in the enzymolysis liquid to obtain the fermentation type small molecular peptide mixed liquid.
Preferably, the mass ratio of the poria cocos to the ginseng, the Chinese yam, the sealwort, the medlar, the dendrobium and the mulberry leaf in the poria cocos bacterial liquid in the step (1) is (150-200): (3-10): (10-20): (10-15): (15-20): (10-20): (10-15).
Preferably, the weight ratio of the poria cocos to water in the poria cocos bacterial liquid in the step (1) is 1: 10.
preferably, the enzymolysis in the step (2) includes performing first enzymolysis on the fermentation broth by using cellulase to obtain a first enzymolysis solution, performing second enzymolysis on the first enzymolysis solution by using pectinase to obtain a second enzymolysis solution, and performing third enzymolysis on the second enzymolysis solution by using papain to obtain the enzymolysis solution;
the pH value of the first enzymolysis is 4.5-6.5, the temperature is 45-55 ℃, and the time is 0.4-2.2 h;
the pH value of the second enzymolysis is 3.0-6.0, the temperature is 15-55 ℃, and the time is 0.4-2.2 h;
the pH value of the third enzymolysis is 6-7, the temperature is 55-65 ℃, and the time is 0.4-2.2 h.
Preferably, the addition amount of the cellulase, the pectinase and the papain in the enzymolysis in the step (2) is 0.1-0.3%.
Preferably, the retaining of step (3) comprises ultrafiltration using an ultrafiltration membrane.
The invention also provides the fermentation type small molecular peptide mixed solution prepared by the preparation method.
The invention also provides application of the small molecule peptide mixed solution in preparation of food or medicines for maintaining healthy blood sugar level.
The invention also provides a food or a medicine for maintaining healthy blood sugar level, and the effective component of the food or the medicine comprises the small molecule peptide mixed liquor.
Preferably, the formulation of the food or pharmaceutical product comprises a solid or liquid agent.
The invention provides a preparation method of a fermentation type small molecular peptide mixed solution for regulating blood sugar, which utilizes a poria cocos bacterial solution, composite probiotics, mulberry leaves, sealwort, dendrobium, schisandra chinensis, astragalus membranaceus, ginseng, Chinese yam and wolfberry fruits to carry out fermentation together, fermentation liquor is subjected to enzymolysis by cellulase, pectinase and papain, and ultrafiltration is carried out to obtain the fermentation type small molecular peptide mixed solution rich in various bioactive factors such as polypeptide, oligosaccharide, saponin and flavone, wherein various bioactive factors have synergistic effect. According to the invention, by selecting and proportioning various components in the fermentation liquor, the small molecular peptide mixed liquor capable of regulating intestinal flora, improving immunity and blood circulation is finally obtained, and the healthy level of blood sugar can be maintained on the whole.
The preparation method disclosed by the invention uses a fermentation technology, utilizes the poria cocos bacterial liquid to ferment various medicinal and edible homologous substances, is simple in material obtaining and obvious in effect, and is easy to prepare and easy to successfully ferment; the raw materials have wide selection sources, low cost, safe components, wide market circulation degree and no toxic or side effect, and the consistent taking of the medicine has the effects of regulating and improving systems such as human digestion, endocrine and the like besides improving the blood sugar level, thereby not only improving the utilization rate of the medicine-food homologous raw materials and reducing the cost of the medicine for improving the blood sugar level, but also greatly increasing the bioavailability of the human body and maintaining the bioactivity of the blood sugar.
The fermentation type small molecular peptide mixed solution is rich in various bioactive factors such as polypeptide, oligosaccharide, saponin, flavone and the like, and can generate remarkable synergistic effect: the flavone has multidirectional effect, can improve blood circulation, can reduce cholesterol, and even contains a PAF anticoagulant factor, thereby greatly reducing the incidence of cardiovascular and cerebrovascular diseases and improving the symptoms of the cardiovascular and cerebrovascular diseases; the oligosaccharide has the effects of improving micro-ecological environment in human body, promoting proliferation of Bacillus bifidus and other beneficial bacteria, reducing intestinal pH value by producing organic acid through metabolism, inhibiting growth of Salmonella and putrefying bacteria in intestine, regulating gastrointestinal function, inhibiting intestinal putrefying substance, preventing and treating constipation, increasing vitamin synthesis, and improving immunity. The fermentation type small molecular peptide mixed liquid has very obvious clinical experiment effect, is prepared into oral liquid, is taken by hyperglycemia patients at different ages at morning and evening once, takes a treatment course for half a month, improves to a certain extent after 4 treatment courses, obviously improves after 8 treatment courses, maintains the blood sugar at a normal level after 10 treatment courses, needs more treatment courses as the ages are larger, improves the mental state and the gastrointestinal function of the patients to a great extent, and has the obvious effect of improving the blood sugar level by 90 percent.
Detailed Description
The invention provides a preparation method of a fermentation type small molecule peptide mixed solution for regulating blood sugar, which comprises the following steps: (1) mixing Poria bacteria liquid and composite probiotic with Ginseng radix, rhizoma Dioscoreae, rhizoma Polygonati, fructus Lycii, herba Dendrobii and folium Mori, and fermenting together to obtain fermentation liquid; the fermentation temperature is 26-28 ℃, the humidity is 62-70%, and the time is 16-24 h; the strains of the composite probiotics comprise: bifidobacterium adolescentis, Bifidobacterium lactis, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus rhamnosus;
(2) sequentially carrying out enzymolysis on the fermentation liquor by using cellulase, pectinase and papain to obtain enzymolysis liquid;
(3) and intercepting components with the molecular weight of less than 2000Da in the enzymolysis liquid to obtain the fermentation type small molecular peptide mixed liquid.
The invention mixes the tuckahoe bacterial liquid and the compound probiotics with ginseng, yam, rhizoma polygonati, medlar, dendrobium and mulberry leaves, and then jointly ferments to obtain fermentation liquid; the fermentation temperature is 26-28 ℃, the humidity is 62-70%, and the time is 16-24 h; the strains of the composite probiotics comprise: lactic acid bacteria, Bifidobacterium lactis, Bifidobacterium longum, and Lactobacillus bulgaricus.
The mass ratio of the poria cocos to the ginseng, the Chinese yam, the sealwort, the wolfberry fruit, the dendrobium and the mulberry leaf in the poria cocos bacterial liquid is preferably (150-200): (3-10): (10-20): (10-15): (15-20): (10-20): (10-15), more preferably (150-190): (3-5): (10-15): (10-14): (10-16): (15-18): (10-15): (10-13).
The composite probiotics are preferably purchased from Shandong Jungle Biotech limited; and the adding mass of the bacterium powder of the composite probiotics is 1 percent of the mass of the fermentation liquor.
The preparation method of the poria cocos bacterial liquid preferably comprises the following steps of crushing poria cocos, mixing with water according to the ratio of 1: 10 by mass ratio. According to the invention, the poria cocos bacterial liquid is used for fermenting various medicinal and edible homologous substances, the materials are simple to obtain, the effect is obvious, the poria cocos bacterial liquid is easy to prepare, and the fermentation is easy to succeed; and the tuckahoe can promote diuresis, eliminate dampness, invigorate spleen and calm heart, and has certain regulation effect on spleen deficiency, anorexia, uneasiness, palpitation and insomnia.
In the invention, the mulberry leaves are suitable for treating the effects of reducing blood sugar and blood fat, resisting inflammation and the like; rhizoma Polygonati has effects of invigorating qi, nourishing yin, invigorating spleen, moistening lung, and invigorating kidney; dendrobe, sweet and light in nature and taste, slightly salty and cold in property, enters stomach, kidney and lung channel, benefits stomach, promotes fluid production, nourishes yin and clears heat, is used for yin deficiency and fluid deficiency, dry mouth, polydipsia, anorexia, retching, asthenic fever after illness and dim and unclear eyes; the ginseng can stimulate the secretion function of an endocrine system, enhance the function of a pituitary adrenal cortex system, has the function of reducing blood sugar, can reduce urine sugar of a light diabetic patient and improve the general symptoms of a moderate diabetic patient by taking the ginseng, so that the symptoms of craving, hyperhidrosis, weakness and the like disappear or are relieved, and the dosage of insulin can be reduced by taking the ginseng; the Chinese yam is sweet, warm, mild and nontoxic, is mainly used for treating impairment of middle warmer, tonifying deficiency and win, removing cold and heat evil, tonifying middle warmer, benefiting strength, being taken for a long time, improving hearing and eyesight, reducing weight, not hunger, prolonging life, tonifying five consumptive diseases and seven injuries, removing cold wind, calming mind, calming soul, tonifying heart-qi deficiency, and keeping heart-hole for many stories; the medlar is sweet in taste and neutral in nature, enters liver and kidney channels, has the effects of tonifying qi and strengthening essence, nourishing liver and kidney, resisting aging, relieving diabetes and warming the body, and the medlar extract can obviously and durably reduce the blood sugar of rats and increase the sugar tolerance and has lower toxicity.
The sources of the traditional Chinese medicines are not particularly limited, and the traditional Chinese medicines can be prepared by using conventional commercially available medicines in the field.
After the fermentation liquor is obtained, the invention carries out enzymolysis on the fermentation liquor by sequentially utilizing cellulase, pectinase and papain to obtain enzymolysis liquid. The enzymolysis preferably comprises the steps of carrying out first enzymolysis on the fermentation liquor by using cellulase to obtain first enzymolysis liquid, carrying out second enzymolysis on the first enzymolysis liquid by using pectinase to obtain second enzymolysis liquid, and carrying out third enzymolysis on the second enzymolysis liquid by using papain to obtain the enzymolysis liquid. The pH value of the first enzymolysis is preferably 4.5-6.5, the temperature is preferably 45-55 ℃, and the time is preferably 0.4-2.2 h; the pH value of the second enzymolysis is preferably 3.0-6.0, the temperature is preferably 15-55 ℃, and the time is preferably 0.4-2.2 h; the pH value of the third enzymolysis is preferably 6-7, the temperature is preferably 55-65 ℃, and the time is preferably 0.4-2.2 h. In the enzymolysis, the addition amounts of the cellulase, the pectinase and the papain are preferably 0.1-0.3%. In the invention, the cellulase and the pectinase carry out wall-breaking enzymolysis on the raw materials, and the papain hydrolyzes macromolecular protein into small peptides.
When the enzymolysis liquid is obtained, the invention intercepts the components with the molecular weight less than 2000Da in the enzymolysis liquid to obtain the fermentation type small molecular peptide mixed liquid. The retention according to the invention preferably comprises ultrafiltration with an ultrafiltration membrane. The fermentation type small molecular peptide mixed solution contains various bioactive factors such as polypeptide, oligosaccharide, saponin, flavone and the like.
The invention also provides the fermentation type small molecular peptide mixed solution prepared by the preparation method. The bioactive factors in the fermented small molecular peptide mixed solution have a synergistic effect, so that the organism is regulated in multiple directions, and the whole body is improved, so that an optimal maintenance mode of the blood sugar level is achieved.
The invention also provides application of the small molecule peptide mixed solution in preparation of food or medicines for maintaining healthy blood sugar level.
The invention also provides a food or a medicine for maintaining healthy blood sugar level, and the effective component of the food or the medicine comprises the small molecule peptide mixed liquor.
The dosage form of the food or pharmaceutical product of the present invention preferably includes a solid agent or a liquid agent, and more preferably includes an oral liquid.
The following examples are provided to describe the fermented small molecule peptide mixture for regulating blood sugar and the preparation method and application thereof in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
Mashing 30g of poria cocos, and mixing with 300ml of water to obtain 300ml of bacterial liquid;
mixing 6g of ginseng, 20g of Chinese yam, 20g of rhizoma polygonati, 20g of schisandra chinensis, 30g of wolfberry fruit, 20g of dendrobium, 20g of mulberry leaf and 300ml of poria cocos liquid, adding 4.54g of composite probiotic bacterium powder (Shandong Junle Biotech Co., Ltd.), fermenting for 24h at room temperature of 27 ℃ and humidity of below 70 wt.% to obtain fermentation liquid, performing enzymolysis on the fermentation liquid for 1.2h by using cellulase at pH 5 and temperature of 50 ℃ to obtain first enzymolysis liquid, performing enzymolysis on the first enzymolysis liquid for 1.2h by using pectinase at pH 4 and temperature of 50 ℃ to obtain second enzymolysis liquid, and performing enzymolysis on the fermentation liquid for 1.2h by using papain at pH 6.5 and temperature of 60 ℃ to obtain third enzymolysis liquid; wherein the dosage of the cellulose is 0.2 percent of the mass of the fermentation liquor; the dosage of the pectinase and the papain is 0.1 percent of the mass of the fermentation liquor.
And (3) performing ultrafiltration on the obtained enzymolysis liquid by using a filter membrane with the molecular weight cutoff of 2000Da through an ultrafiltration membrane to obtain 50ml of fermentation type micromolecular peptide mixed liquid.
30 SPF-grade Kunming male mice (Liaoning Biotechnology Limited) are selected, and the body mass is (25 +/-5) g. After the mice were purchased, the mice were divided into a blank group, a model group and a dosing group. The model group mice are molded by adopting a mode of injecting Streptozotocin (STZ) solution into the abdominal cavity, the concentration is 5mg/ml, the injection amount is 35mg/kg, the injection is continuously carried out for 3 days, the mice are fed with tap water and are fed with high-fat high-sugar feed, and when the blood sugar of the mice rises to more than 11.1mmol/L, the molding is successful.
The mice in the blank group are injected with sodium citrate buffer solution with the same amount per day in the abdominal cavity, and the mice drink tap water and conventional feed. And (3) respectively adopting a tail vein blood sampling method before and after modeling, and measuring blood sugar by using a glucometer. After fasting for 8h without water deprivation, the blood glucose concentration of the mice was measured. After the molding was successfully performed, 80ml/kg of the drug was administered by gavage in the afternoon administration group every day, and after 8 weeks of continuous gavage of the same dose of physiological saline in the blank group and the model group, the blood glucose values were measured, and the results are shown in table 1.
TABLE 1 blood glucose values (mmol/L) for different groups of mice
P <0.01 compared to model group.
As is clear from the experimental data shown in Table 1, the use of the fermented small molecule peptide mixture of the present invention can reduce the blood glucose level of type 2 diabetic mice successfully molded
Example 2
The preparation protocol was the same as in example 1, except that the formulation was different from that of example 1: 330ml of poria cocos bacterial liquid (33g of poria cocos and 330ml of water), 8g of ginseng, 26g of Chinese yam, 28g of rhizoma polygonati, 28g of schisandra chinensis, 34g of wolfberry fruit, 28g of dendrobium nobile, 24g of mulberry leaf and 5.26g of composite probiotic bacteria powder (Shandong bacteria-le biotechnology limited).
In this example, 30 mice similar to those in example 1 were used, and the mice were divided into a blank group, a model group and an administration group, and the molding method was the same as that in example 1.
After successful molding, the blank group and the model group were subjected to gavage administration of 80ml/kg of normal saline, and after 8 weeks, the blood glucose values were measured, and the results are shown in table 2.
TABLE 2 blood glucose values (mmol/L) for different groups of mice
| Before molding | After molding | After administration | |
| Blank group | 6.54±0.16 | 6.57±0.13 | 6.60±0.13 |
| Model set | 6.44±0.14 | 23.56±0.85 | 25.77±1.85 |
| Administration set | 6.43±0.12 | 24.66±1.25 | 16.53±0.56* |
P <0.01 compared to model group.
As is clear from the experimental data shown in Table 2, the blood glucose level of the type 2 diabetic mice successfully molded can be reduced to some extent by using the fermented small molecule peptide mixture of the present invention
Example 3
The preparation protocol was the same as in example 1, except that the formulation was different from that of example 1: 380ml of tuckahoe bacterial liquid (38g of tuckahoe and 380ml of water) are fermented with 10g of ginseng, 30g of yam, 26g of rhizoma polygonati, 32g of schisandra, 36g of medlar, 30g of dendrobium, 26g of mulberry leaf and 5.93g of composite probiotic bacterial powder (Shandong Junle Biotech Co., Ltd.).
The same molding experiment was performed using the same number of mice as in example 2.
After successful molding, the blank group and the model group were subjected to gavage administration of 80ml/kg of normal saline, and after 8 weeks, the blood glucose values were measured, and the results are shown in table 3.
TABLE 3 blood glucose values (mmol/L) for different groups of mice
| Before molding | After molding | After administration | |
| Blank group | 6.39±0.12 | 6.43±0.07 | 6.52±0.06 |
| Model set | 6.25±0.06 | 21.80±0.82 | 25.95±1.93 |
| Administration set | 6.20±0.06 | 21.54±1.02 | 11.87±1.59* |
P <0.01 compared to model group.
As can be seen from the experimental data shown in table 3, the blood glucose level of the type 2 diabetic mice successfully molded can be significantly reduced by using the fermented small molecule peptide mixture of the present invention.
Example 4
Selecting two groups of people of 35-55 years old, diabetic patients and 80 people according to the ages, wherein each group comprises 40 people, 35-45 years old is one group, and 46-55 years old is one group. 50ml of the small molecule peptide mixed solution prepared in the third example is taken twice a day in the morning and at night, and the blood sugar value change of 4 courses, 8 courses and 10 courses before taking is recorded, as shown in table 4.
TABLE 4 blood sugar values (mmol/L) for persons of different age groups
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A preparation method of a fermentation type small molecule peptide mixed solution for regulating blood sugar is characterized by comprising the following steps: (1) mixing Poria bacteria liquid and composite probiotic with Ginseng radix, rhizoma Dioscoreae, rhizoma Polygonati, fructus Lycii, herba Dendrobii and folium Mori, and fermenting together to obtain fermentation liquid; the fermentation temperature is 26-28 ℃, the humidity is 62-70%, and the time is 16-24 h; the strains of the composite probiotics comprise: bifidobacterium adolescentis, Bifidobacterium lactis, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus rhamnosus;
(2) sequentially carrying out enzymolysis on the fermentation liquor by using cellulase, pectinase and papain to obtain enzymolysis liquid;
(3) and intercepting components with the molecular weight of less than 2000Da in the enzymolysis liquid to obtain the fermentation type small molecular peptide mixed liquid.
2. The preparation method according to claim 1, wherein the mass ratio of the poria cocos to the ginseng, the yam, the polygonatum rhizome, the wolfberry fruit, the dendrobium and the mulberry leaf in the poria cocos bacterial liquid in the step (1) is (150-200): (3-10): (10-20): (10-15): (15-20): (10-20): (10-15).
3. The method according to claim 1 or 2, wherein the mass ratio of Poria cocos to water in the Poria cocos solution of step (1) is 1: 10.
4. the preparation method according to claim 1, wherein the enzymolysis in step (2) comprises performing a first enzymolysis on the fermentation broth with cellulase to obtain a first enzymolysis solution, performing a second enzymolysis on the first enzymolysis solution with pectinase to obtain a second enzymolysis solution, and performing a third enzymolysis on the second enzymolysis solution with papain to obtain the enzymolysis solution;
the pH value of the first enzymolysis is 4.5-6.5, the temperature is 45-55 ℃, and the time is 0.4-2.2 h;
the pH value of the second enzymolysis is 3.0-6.0, the temperature is 15-55 ℃, and the time is 0.4-2.2 h;
the pH value of the third enzymolysis is 6-7, the temperature is 55-65 ℃, and the time is 0.4-2.2 h.
5. The preparation method according to claim 1 or 4, wherein the addition amount of the cellulase, the pectinase and the papain in the enzymolysis in the step (2) is 0.1-0.3%.
6. The method of claim 1, wherein the retaining of step (3) comprises ultrafiltration using an ultrafiltration membrane.
7. A fermented small molecule peptide mixture prepared by the method according to any one of claims 1 to 6.
8. Use of the small molecule peptide mixture of claim 7 for the preparation of a food or pharmaceutical product for maintaining healthy blood glucose levels.
9. A food or medicine for maintaining healthy blood sugar level, wherein the effective component of the food or medicine comprises the small molecule peptide mixture of claim 7.
10. The food or pharmaceutical product of claim 9, wherein the food or pharmaceutical product is in a dosage form comprising a solid or liquid dosage form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110356523.XA CN113017092B (en) | 2021-04-01 | 2021-04-01 | Fermentation type small molecule peptide mixed solution for regulating blood sugar and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110356523.XA CN113017092B (en) | 2021-04-01 | 2021-04-01 | Fermentation type small molecule peptide mixed solution for regulating blood sugar and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113017092A true CN113017092A (en) | 2021-06-25 |
| CN113017092B CN113017092B (en) | 2022-07-01 |
Family
ID=76454014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110356523.XA Active CN113017092B (en) | 2021-04-01 | 2021-04-01 | Fermentation type small molecule peptide mixed solution for regulating blood sugar and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113017092B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114042132A (en) * | 2021-12-27 | 2022-02-15 | 广东安道医疗器械有限公司 | Application of calyx seu fructus physalis alcohol extract in preparing diabetes medicine |
| CN115944081A (en) * | 2023-01-13 | 2023-04-11 | 广东省农业科学院蚕业与农产品加工研究所 | Mulberry leaf food and preparation method thereof |
| CN116784473A (en) * | 2022-05-19 | 2023-09-22 | 沈启良 | Novel functional food and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6099844A (en) * | 1994-08-22 | 2000-08-08 | Triarco Industries, Inc. | Increasing yield of extractable substances from botanicals with an enzyme composition |
| CN1429594A (en) * | 2001-12-29 | 2003-07-16 | 上海展望生物制品有限公司 | Raw material in plant for regulating blood sugar extracted by using super critical CO2 extraction method and its preparation method |
| CN1698871A (en) * | 2005-05-25 | 2005-11-23 | 北京华昱安然医药科技有限公司 | Health product for assisting blood sugar-decreasing function and its preparation method |
| CN105796924A (en) * | 2016-05-16 | 2016-07-27 | 刘世超 | Officinal Dendrobium Stem granules and preparation method thereof |
| CN109832619A (en) * | 2019-01-04 | 2019-06-04 | 中国食品发酵工业研究院有限公司 | A kind of Poria cocos fermented product and preparation method thereof |
| CN110338402A (en) * | 2018-04-08 | 2019-10-18 | 王康学 | A kind of compound probiotic powder and preparation method thereof |
-
2021
- 2021-04-01 CN CN202110356523.XA patent/CN113017092B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6099844A (en) * | 1994-08-22 | 2000-08-08 | Triarco Industries, Inc. | Increasing yield of extractable substances from botanicals with an enzyme composition |
| CN1429594A (en) * | 2001-12-29 | 2003-07-16 | 上海展望生物制品有限公司 | Raw material in plant for regulating blood sugar extracted by using super critical CO2 extraction method and its preparation method |
| CN1698871A (en) * | 2005-05-25 | 2005-11-23 | 北京华昱安然医药科技有限公司 | Health product for assisting blood sugar-decreasing function and its preparation method |
| CN105796924A (en) * | 2016-05-16 | 2016-07-27 | 刘世超 | Officinal Dendrobium Stem granules and preparation method thereof |
| CN110338402A (en) * | 2018-04-08 | 2019-10-18 | 王康学 | A kind of compound probiotic powder and preparation method thereof |
| CN109832619A (en) * | 2019-01-04 | 2019-06-04 | 中国食品发酵工业研究院有限公司 | A kind of Poria cocos fermented product and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 于婷等: "发酵技术改善中药降血糖作用的研究进展", 《延边大学农学学报》 * |
| 刘玉田: "《淀粉类食品新工艺与新配方》", 30 June 2002, 山东科学技术出版社 * |
| 李梦颖等: "茯苓-丹参共发酵体系及其产物对糖尿病小鼠降血糖作用的影响", 《食品研究与开发》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114042132A (en) * | 2021-12-27 | 2022-02-15 | 广东安道医疗器械有限公司 | Application of calyx seu fructus physalis alcohol extract in preparing diabetes medicine |
| CN116784473A (en) * | 2022-05-19 | 2023-09-22 | 沈启良 | Novel functional food and preparation method thereof |
| CN115944081A (en) * | 2023-01-13 | 2023-04-11 | 广东省农业科学院蚕业与农产品加工研究所 | Mulberry leaf food and preparation method thereof |
| CN115944081B (en) * | 2023-01-13 | 2024-02-09 | 广东省农业科学院蚕业与农产品加工研究所 | Mulberry leaf food and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113017092B (en) | 2022-07-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113017092B (en) | Fermentation type small molecule peptide mixed solution for regulating blood sugar and preparation method and application thereof | |
| US9950024B2 (en) | Traditional Chinese medicine composition and the use thereof | |
| CN112089775A (en) | NMN beneficial bacterium health composition and preparation method and application thereof | |
| CN110338402A (en) | A kind of compound probiotic powder and preparation method thereof | |
| CN106266229B (en) | Enzyme with blood replenishing effect and preparation method thereof | |
| CN108244432A (en) | A kind of fermentation Cordyceps militaris probiotic beverage and preparation method thereof | |
| CN109528814B (en) | Microecological preparation of lactobacillus fermented astragalus membranaceus as well as preparation method and application of microecological preparation | |
| JP2004505625A (en) | Conversion and modification of natural drugs by co-fermentation culture of intestinal symbiotic organisms | |
| CN106418525A (en) | Fermenting folium mori chewable tablet and preparation method thereof | |
| CN110604800A (en) | Alcohol-dispelling liver-protecting plant beverage for abstinence of alcohol and preparation method thereof | |
| CN110547447A (en) | Royal jelly collagen fruit enzyme and preparation method thereof | |
| CN104187741A (en) | Total nutrient formula food for heart disease | |
| CN104256656A (en) | Full-nutrition formulated food for fatty acid metabolic disorder | |
| CN109966427A (en) | A kind of fermentation living bacterial liquid and preparation method thereof treated tumour and improve immunity of organisms | |
| CN115777931A (en) | Dietary supplement for coordinating intestines and stomach and preparation method and application thereof | |
| CN105749099B (en) | A kind of probiotics fermention Chinese medicine composition and its preparation method and application for treating gastric cancer | |
| CN113144167A (en) | Fermented small-molecule peptide mixed solution with uric acid reducing effect and preparation method and application thereof | |
| CN110801002A (en) | Pilose antler nourishing enzyme and preparation method thereof | |
| KR20200074061A (en) | Composition for improving cognitive function | |
| CN111264866A (en) | Mammary gland fibroma medical formula food and preparation method thereof | |
| CN111116708A (en) | Compound, traditional Chinese medicine probiotic fermentation product and application of compound and traditional Chinese medicine probiotic fermentation product in preparation of medicine or health-care product with effects of dispelling effects of alcohol and protecting stomach | |
| CN104222293A (en) | Chewable Qi tonifying yoghourt tablet and production method thereof | |
| KR102676880B1 (en) | Method for manufacturing herbal medicines for improving sleep disorders and herbal medicines manufactured thereby | |
| CN114098069B (en) | Lactic acid bacteria combined fermentation product and preparation method thereof | |
| CN102631487B (en) | Traditional Chinese medicine composition capable of improving immunity and resisting fatigue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |