CN106266229B - Enzyme with blood replenishing effect and preparation method thereof - Google Patents
Enzyme with blood replenishing effect and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention discloses a ferment with blood replenishing effect, which is prepared by fermenting the following raw materials and microorganisms in parts by weight: 240-360 parts of longan aril, 160-240 parts of mulberry, 160-240 parts of wolfberry fruit, 160-240 parts of raspberry, 0.04-0.16 part of yeast and 1.4-5.4 parts of lactic acid bacteria. The efficacy experiment shows that the four raw materials of the longan pulp, the mulberry, the medlar and the raspberry can play a synergistic interaction role under the specific dosage proportion of the invention, and the invention has obvious blood enriching efficacy. In addition, the four medicinal and edible raw materials are prepared into the enzyme with the blood replenishing effect, compared with the raw materials, the enzyme has better taste and more convenient administration, beneficial components such as extracellular polysaccharide, lactobacillus and the like are obviously increased, the SOD activity is increased, and the enzyme has better health care effect.
Description
Technical Field
The invention relates to a ferment with a blood replenishing effect and a preparation method thereof, and belongs to the field of medicines.
Background
Blood deficiency belongs to the category of traditional Chinese medicine diseases, refers to a pathological state that blood is insufficient, nutrition and moistening functions of blood are reduced, so that viscera, meridians, body and organs and the like are not nourished, and clinically, the blood deficiency is mainly manifested by pale or sallow complexion, lusterless hair, pale lips, tongue and nails, dizziness, blurred vision, severe fatigue, palpitation, amnesia, insomnia, hypodynamia and the like. The blood deficiency syndrome is not only seen in anemia but also in other diseases such as coronary heart disease, neurosis, viral myocarditis, etc. in the distribution of western medicine.
Modern researches have proved that blood deficiency can cause the decrease of hematopoietic function of bone marrow and peripheral red blood cell content and function, the decrease of immunologic function, the decrease of free radical scavenging ability of organism, even the disorder of secretion of various hormones, the influence of fertility and other serious consequences, and the harm is very large (Guo Ping, blood deficiency syndrome pathological research progress [ J ]. Shandong Chinese medicine journal, 2006, 25 (9): 643-645). Because the pathogenesis of blood deficiency is complex and is often accompanied with pathological changes of a plurality of systems and organs, most of the existing treatment means have poor effect, and some medicines have obvious adverse reaction. For example, for iron deficiency anemia, iron preparations are currently and frequently administered clinically for treatment; however, most of the iron ingested by the human body is difficult to be fully utilized, only a small amount of iron can be absorbed by the body and plays a physiological role, and in addition, the iron preparation often has a gastrointestinal tract stimulation effect, so that the absorption of the medicament is further hindered, and the effect of treating anemia is very small.
Therefore, it is an urgent problem to provide a medicine, health product or food with significant blood-enriching efficacy and no obvious toxic and side effects.
The ferment is a functional microbial fermentation product which is produced by taking one or more fresh vegetables, fruits, mushrooms, Chinese herbal medicines and the like as raw materials and fermenting through a plurality of beneficial bacteria and contains rich nutrient components such as vitamins, enzymes, minerals, secondary metabolites and the like. The ferment not only preserves the inherent nutrient substances in the fermentation raw materials, but also can improve some original bad flavors of the fermentation raw materials, and more importantly, generates some new functional components in the fermentation process, thereby having good health care effect on human bodies.
However, microbial fermentation is a complex process, and factors affecting the quality of enzyme products are many, for example, process parameters such as fermentation strains and inoculation amount have a particularly significant effect on the enzyme quality, and it is very difficult for technicians to determine appropriate fermentation conditions, so as to obtain an enzyme product with good flavor and high content of functional components.
Disclosure of Invention
The invention aims to provide a ferment with blood replenishing effect and a preparation method thereof.
The invention provides a ferment with blood replenishing effect, which is prepared by fermenting the following raw materials and microorganisms in parts by weight: 240-360 parts of longan aril, 160-240 parts of mulberry, 160-240 parts of wolfberry fruit, 160-240 parts of raspberry, 0.04-0.16 part of yeast and 1.4-5.4 parts of lactic acid bacteria.
Preferably, the microbial fertilizer is prepared by fermenting the following raw materials and microorganisms in parts by weight: 300 parts of longan aril, 200 parts of mulberry, 200 parts of medlar, 200 parts of raspberry, 0.1 part of yeast and 3 parts of lactobacillus.
Further preferably, the lactic acid bacteria are selected from one or more of lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus and bifidobacterium adolescentis.
The invention provides a preparation method of the ferment, which comprises the following steps:
a. weighing raw materials in weight ratio, crushing and pulping, carrying out enzymolysis by pectinase, separating to obtain an enzymolysis liquid, inactivating enzyme, sterilizing and cooling;
b. fermenting the enzymolysis liquid with saccharomycetes, and separating to obtain supernatant for later use;
c. inoculating lactobacillus into the supernatant, fermenting for the second time, and reserving fermentation liquor for later use;
d. and centrifuging the fermentation liquor, and taking supernatant to obtain the ferment stock solution.
Further, the conditions of the pectinase enzymolysis are as follows: adjusting the pH value to 3-4, adding 0.2-0.5% of pectinase according to the weight ratio at the temperature of 45-50 ℃, and carrying out enzymolysis for 2-4 hours.
Further, the yeast fermentation conditions are as follows: the fermentation temperature is 20-30 ℃, and the fermentation time is 24-72 hours.
Further, the conditions of the second fermentation are as follows: the fermentation temperature is 30-35 ℃, and the fermentation time is 12-36 hours.
The invention provides application of the ferment in preparation of a medicine, food or health-care product with a blood replenishing effect.
Furthermore, the medicine, the health-care product or the food has the effect of treating and/or preventing blood deficiency.
Furthermore, the medicine, the health-care product or the food has the effect of treating and/or preventing hemorrhagic blood deficiency and chemical blood deficiency.
The invention provides a ferment with blood replenishing effect. The ferment has reasonable raw material formula, and sweet and warm arillus longan is used as the monarch drug, has effects of invigorating heart and spleen, nourishing blood and tranquillizing, and can be used for treating deficiency of qi and blood, palpitation, amnesia, insomnia, blood deficiency and sallow complexion; the mulberry fruit, which is sweet and sour in nature and cold in nature, is used as the minister agent to enter the liver and kidney channels, nourish yin and blood, promote the production of body fluid and moisten the intestines. Dizziness, soreness of waist, tinnitus, premature gray hair, insomnia, dreaminess, thirst due to body fluid consumption, diabetes, and constipation due to intestinal dryness. The longan pulp can greatly tonify qi and blood, the mulberry can nourish yin and blood, and the two medicines are combined to supplement qi, blood, yin and yang; moreover, the medicine is warm and cold, and does not cause dryness-heat and deficiency-cold after long-term administration. The sweet and mild medlar is used as an adjuvant, so that the liver and kidney are nourished, and the essence and the eyesight are benefited and improved; the raspberry with the sweet and sour taste is used for tonifying the kidney, securing essence and reducing urination. The four ingredients are used together to tonify the innate kidney and the acquired spleen; not only can nourish the blood of heart and liver, but also tonify the yin of liver and kidney, calm the cold and heat, balance qi, blood, yin and yang, strengthen the strength of invigoration and simultaneously strengthen the astringency.
The efficacy experiment shows that the four raw materials of the longan pulp, the mulberry, the medlar and the raspberry can play a synergistic interaction role under the specific dosage proportion of the invention, and the invention has obvious blood enriching efficacy.
In addition, the four medicinal and edible raw materials are prepared into the enzyme with the blood replenishing effect, compared with the raw materials, the enzyme has better taste and more convenient administration, beneficial components such as extracellular polysaccharide, lactobacillus and the like are obviously increased, the SOD activity is increased, and the enzyme has better health care effect.
Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Detailed Description
The raw materials and equipment used in the embodiment of the present invention are known products and obtained by purchasing commercially available products.
Example 1 preparation of the enzyme of the invention
Weighing 150g of arillus longan, 100g of mulberry, 100g of wolfberry fruit and 100g of raspberry, removing impurities, cleaning, adding 2-4 times of purified water which is cooled after being boiled and sterilized, soaking for 30 minutes, crushing and pulping;
adjusting the pH value to 3-4, adding 0.2-0.5% of pectinase according to the weight ratio at the temperature of 45-50 ℃, carrying out enzymolysis for 2-4 hours, separating and taking juice, boiling for 10 minutes, sterilizing, and cooling to room temperature;
adding 0.05g of yeast into the enzymolysis liquid, maintaining the fermentation temperature at 20-30 ℃, fermenting for 24-72 hours, and separating supernatant for later use;
inoculating 1.5g of lactobacillus (one or more mixed bacteria of lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus and bifidobacterium adolescentis) into the supernatant, maintaining the fermentation temperature at 30-35 ℃, and fermenting for 12-36 hours for later use;
centrifuging, taking supernatant, filling, and storing at 4 ℃ to obtain the ferment stock solution.
The beneficial effects of the present invention are demonstrated by the following experimental examples.
Experimental example 1 blood-replenishing Effect of the raw materials of the present invention
1. Preparation of test solutions
Blood enriching formula extract: soaking arillus longan 30g, Mori fructus 20g, fructus Lycii 20g, and Rubi fructus 20g in 2 times volume of water for 30 min, decocting with 6 times volume of water for 2 times for 30 min, mixing decoctions, filtering, and concentrating under reduced pressure to obtain 1g crude drug per 1 ml.
Longan pulp extract: taking 90g of longan pulp, and preparing by the same method.
Mulberry extract: taking 90g of longan pulp, and preparing by the same method.
Wolfberry fruit extract: the preparation method is the same with that of the wolfberry fruit of 90 g.
And (3) raspberry extract: preparing 90g of raspberry by the same method.
2. Study on the Effect of blood loss mice
Taking 56 mice (Kunming, first grade, half of male and female, weight of 18-22 g) and half of male and female, randomly dividing into 7 groups, respectively filling blood enriching formula extract (1.35g/kg), arillus longan extract (1.35g/kg), mulberry extract (1.35g/kg), medlar extract (1.35g/kg), raspberry extract (1.35g/kg) or physiological saline with the same volume, continuously taking for 7 days, and bleeding the retroorbital veins of 7 days after administration for about 0.6ml to cause a mouse blood loss model, wherein the normal control group does not bleed blood. The administration is continued for 7 days after blood loss, after the last administration is carried out for 1.5h, blood is taken from the orbit, heparin is used for anticoagulation, and RBC and Hb values of the mice are measured by an automatic hematology analyzer. The results are shown in Table 1.
TABLE 1 results of the study of the effect of the composition of the present invention on blood-loss deficient mice: (
n＝8)
| Group of | RBC(1012/L) | Hb(g/L) |
| Blood-enriching formula extract | 8.72±0.73** | 151.05±9.03* |
| Arillus longan extract | 8.14±0.75** | 148.03±9.82 |
| Mulberry extract | 8.25±0.86** | 147.88±12.86* |
| Fructus Lycii extract | 5.33±0.88 | 130.84±11.28 |
| Raspberry extract | 5.22±0.87 | 137.02±13.08 |
| Normal control | 9.27±0.51** | 151.23±9.02* |
| Model comparison | 5.28±0.87 | 132.26±17.21 |
Note: p < 0.05, P < 0.01, compared to model control
The experimental results are as follows: after the mice are exsanguinated and modeled, the mice have the symptoms of blood deficiency, such as slow movement, dyspnea, erect fluffy herb, little luster, pale eyes and tails, and the like; compared with the normal group, the number of the red blood cells and the content of the hemoglobin in the model group are both obviously reduced, which indicates that the model building is successful.
The blood-enriching square group mice are agile in action, normal in hair color and not obviously different from normal group mice; compared with a model group, the number of red blood cells and the content of hemoglobin of the blood enriching formula group are both obviously improved, and the composition has an obvious treatment effect on hemorrhagic blood deficiency.
In addition, the number of red blood cells or hemoglobin content of mice cannot be effectively increased by using the wolfberry extract or the raspberry extract alone compared with the model group; the number of red blood cells or the hemoglobin content of the mice is lower than that of the blood enriching formula of the invention by using the longan pulp extract or the mulberry extract alone.
The results show that the four raw materials of the invention play a synergistic effect and have obvious effect of treating blood loss type blood deficiency.
3. Study on the Effect of chemically blood-deficient mice
Taking 56 mice (Kunming, first level, each half of male and female, the body weight is 18-22 g), dividing the half of male and female into 7 groups at random, administrating the groups as above, injecting acetylphenylhydrazine 20mg/kg and 40mg/kg subcutaneously on the 3 rd day and the 6 th day of administration, injecting cyclophosphamide 40mg/kg intraperitoneally for 5 consecutive days from the 3 rd day, taking blood from the orbit after 1.5h of the last administration, anticoagulating heparin, and measuring WBC, RBC, Hb and HCT values by using an automatic hematology analyzer. The mice were sacrificed after blood collection and the spleen was weighed. The results are shown in Table 2.
TABLE 2 results of the study of the effect of the composition of the present invention on chemically blood-deficient mice (
n＝8)
Note: p < 0.05, P < 0.01, compared to model control
The experimental results are as follows: after the mice are subjected to combined molding by the acetylphenylhydrazine and the cyclophosphamide, symptoms of hypokinesia, dyspnea, fluffy herb erection, little luster, pale eyes, ears, tails and the like due to blood deficiency begin to appear on the 4 th day. None of the animals in each experimental group died.
The number of red blood cells, white blood cells, hemoglobin content, hematocrit and spleen weight were all significantly reduced in the model group compared to the normal group. Compared with a model group, the blood enriching formula group has the advantages that the red blood cell number, the white blood cell number, the hemoglobin content, the hematocrit and the spleen weight are all obviously improved, and the effects of resisting bone marrow inhibition caused by cyclophosphamide and promoting the hematopoietic function are shown.
In addition, compared with the single use of the wolfberry fruit extract, the raspberry extract, the longan pulp extract or the mulberry extract, the blood enriching formula group mouse has the advantages that all indexes are in the best level, and the four raw materials play a synergistic effect and have the obvious effect of treating chemical blood deficiency.
Experimental example 2 quality evaluation of enzyme of the present invention
The ferments of the invention prepared according to example 1 were subjected to quality evaluation and the results are shown in table 3.
TABLE 3 evaluation results of enzyme of the present invention
Injecting: % means g/100 ml.
The determination result shows that compared with the raw materials, the enzyme disclosed by the invention is better in taste and more convenient to take, beneficial components such as extracellular polysaccharide and lactic acid bacteria are obviously increased, and the SOD activity is increased.
Claims (9)
1. A ferment with blood replenishing effect is characterized in that: the microbial fertilizer is prepared by fermenting the following raw materials and microorganisms in parts by weight: 300 parts of longan aril, 200 parts of mulberry, 200 parts of medlar, 200 parts of raspberry, 0.1 part of yeast and 3 parts of lactobacillus.
2. The ferment of claim 1, wherein: the lactobacillus is selected from one or more of Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus and Bifidobacterium adolescentis.
3. A method for preparing the enzyme of claim 1 or 2, which comprises: the method comprises the following steps:
a. weighing raw materials in weight ratio, crushing and pulping, carrying out enzymolysis by pectinase, separating to obtain an enzymolysis liquid, inactivating enzyme, sterilizing and cooling;
b. fermenting the enzymolysis liquid with saccharomycetes, and separating to obtain supernatant for later use;
c. inoculating lactobacillus into the supernatant, fermenting for the second time, and reserving fermentation liquor for later use;
d. and centrifuging the fermentation liquor, and taking supernatant to obtain the ferment stock solution.
4. The method of claim 3, wherein: the conditions of the pectinase enzymolysis are as follows: adjusting the pH value to 3-4, adding 0.2-0.5% of pectinase according to the weight ratio at the temperature of 45-50 ℃, and carrying out enzymolysis for 2-4 hours.
5. The method of claim 3, wherein: the yeast fermentation conditions are as follows: the fermentation temperature is 20-30 ℃, and the fermentation time is 24-72 hours.
6. The method of claim 3, wherein: the conditions of the second fermentation are as follows: the fermentation temperature is 30-35 ℃, and the fermentation time is 12-36 hours.
7. Use of the ferment of claim 1 or 2 in the preparation of a medicament, food or health product having a blood-enriching effect.
8. Use according to claim 7, characterized in that: the medicine, health-care product or food has the effect of treating and/or preventing blood deficiency.
9. Use according to claim 8, characterized in that: the medicine, health product or food has effects of treating and/or preventing blood loss type blood deficiency and chemical blood deficiency.
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| CN106822601A (en) * | 2017-02-07 | 2017-06-13 | 中山火炬职业技术学院 | A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof |
| CN106962925A (en) * | 2017-03-30 | 2017-07-21 | 程君 | Make the composition and its method of ferment |
| CN109907307A (en) * | 2017-12-13 | 2019-06-21 | 山东博奥克生物科技有限公司 | A kind of mulberry enzyme |
| CN107997150A (en) * | 2018-01-25 | 2018-05-08 | 福建新味食品有限公司 | A kind of processing method of vegetables and fruits enzyme liquid and application |
| CN108887674A (en) * | 2018-07-10 | 2018-11-27 | 集美大学 | A kind of preparation method of lycium ruthenicum composite enzyme liquid |
| CN110089744A (en) * | 2019-06-05 | 2019-08-06 | 重庆康菌泰生物科技股份有限公司 | A kind of raspberry comprehensive enzyme and preparation method thereof |
| CN112616993A (en) * | 2020-12-25 | 2021-04-09 | 黑龙江省京福龙农牧科技开发有限公司 | Enzyme for pets and preparation method thereof |
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| CN104522783A (en) * | 2014-12-30 | 2015-04-22 | 北海万物盛生物技术开发有限公司 | Chinese wampee enzyme drink and preparation method thereof |
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| CN104522783A (en) * | 2014-12-30 | 2015-04-22 | 北海万物盛生物技术开发有限公司 | Chinese wampee enzyme drink and preparation method thereof |
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