CN106822601A - A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof - Google Patents
A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof Download PDFInfo
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- CN106822601A CN106822601A CN201710067067.0A CN201710067067A CN106822601A CN 106822601 A CN106822601 A CN 106822601A CN 201710067067 A CN201710067067 A CN 201710067067A CN 106822601 A CN106822601 A CN 106822601A
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- 239000000843 powder Substances 0.000 title claims abstract description 36
- 241000026010 Dendrobium candidum Species 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 56
- 229910052742 iron Inorganic materials 0.000 claims abstract description 28
- 241001480055 Quercus mongolica Species 0.000 claims abstract description 26
- 239000004575 stone Substances 0.000 claims abstract description 26
- 238000000227 grinding Methods 0.000 claims abstract description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 12
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 11
- 239000002002 slurry Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000004108 freeze drying Methods 0.000 claims abstract description 8
- 239000004310 lactic acid Substances 0.000 claims abstract description 8
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 8
- 238000010009 beating Methods 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 241001076416 Dendrobium tosaense Species 0.000 claims description 9
- 235000014101 wine Nutrition 0.000 claims description 9
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 6
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 6
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 6
- 240000006365 Vitis vinifera Species 0.000 claims description 6
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 6
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 6
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 230000010355 oscillation Effects 0.000 claims description 5
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 235000019990 fruit wine Nutrition 0.000 claims description 3
- 240000004638 Dendrobium nobile Species 0.000 claims description 2
- 239000003963 antioxidant agent Substances 0.000 claims description 2
- 230000003078 antioxidant effect Effects 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 235000013402 health food Nutrition 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000233855 Orchidaceae Species 0.000 claims 1
- 239000008267 milk Substances 0.000 claims 1
- 210000004080 milk Anatomy 0.000 claims 1
- 235000013336 milk Nutrition 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 8
- 230000003026 anti-oxygenic effect Effects 0.000 abstract description 3
- 238000000855 fermentation Methods 0.000 description 10
- 230000004151 fermentation Effects 0.000 description 10
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 4
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 3
- 230000003064 anti-oxidating effect Effects 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 238000003908 quality control method Methods 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241001523681 Dendrobium Species 0.000 description 1
- 240000001008 Dimocarpus longan Species 0.000 description 1
- 235000000235 Euphoria longan Nutrition 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 208000031971 Yin Deficiency Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Public Health (AREA)
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- Medicinal Chemistry (AREA)
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Abstract
The invention discloses a kind of preparation method of dendrobium candidum ferment Ultramicro-powder, step is as follows：(1) iron sheet stone Mongolian oak is taken, 1~4 times of amount water is added, is soaked 10~30 minutes, crushing and beating, sterilizing obtains concoction；(2) in step (1) gained concoction, saccharomycete is added by weight 0.05~0.15%, is fermented 24~72 hours under conditions of being 20~30 DEG C in temperature, obtain liquid；Again in liquid, lactic acid bacteria is added by weight 2~5%, fermented 12~36 hours under conditions of being then 30~35 DEG C in temperature, obtain slurries；(3) step (2) gained slurries, freeze-drying are taken；(4) ultramicro grinding, you can.Dendrobium candidum ferment Ultramicro-powder prepared the invention also discloses preceding method and application thereof.The dendrobium candidum ferment Ultramicro-powder mouthfeel that the inventive method is prepared is excellent, and antioxygenic property is also significantly greater than dendrobium candidum medicinal material, and application prospect is excellent.
Description
Technical field
The present invention relates to a kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof.
Background technology
Dendrobium candidum medicinal material is dry for orchid dendrobium candidum Dendrobium officinale Kimum et Migo's
Dry stem, its nature and flavor are sweet, are slightly cold.Return helmet, kidney channel, reinforcing stomach reg fluid, nourishing Yin and clearing heat.For pyreticosis Tianjin wound, dry polydipsia, stomach-Yin is not
Foot, deficiency of food is retched, and abnormal heat is not moved back after being ill, fire excess from yin deficiency, and hectic fever due to yin labor heat, mesh is secretly failed to understand, muscles and bones impotence.
At present, dendrobium candidum is normally used as medicinal material and directly uses, and mouthfeel is not good, and drug effect also needs further to be carried
It is high.
The content of the invention
The invention provides a kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof.
Present invention firstly provides the preparation method of dendrobium candidum ferment Ultramicro-powder, step is as follows：
(1) iron sheet stone Mongolian oak is taken, 1~4 times of amount water is added, is soaked 10~30 minutes, crushing and beating, sterilizing obtains concoction；
(2) in step (1) gained concoction, saccharomycete is added by weight 0.05~0.15%, is 20~30 in temperature
Fermented 24~72 hours under conditions of DEG C, obtain liquid；Again in liquid, lactic acid bacteria is added by weight 2~5%, then in temperature
Spend to be fermented 12~36 hours under conditions of 30~35 DEG C, obtain slurries；
(3) step (2) gained slurries, freeze-drying are taken；
(4) ultramicro grinding, you can.
In step (1), the dendrobium candidum is orchid dendrobium candidum Dendrobium officinale Kimum
Et Migo's dries stem or fresh stem.
In step (1), the dendrobium candidum is orchid dendrobium candidum Dendrobium officinale Kimum
When drying stem of et Migo, adds 2~4 times of water of amount；The dendrobium candidum is orchid dendrobium candidum Dendrobium
During the fresh stem of officinale Kimum et Migo, 1~2 times of water of amount is added.
In step (1), the mode of the sterilizing is to boil 10 minutes.
In step (2), the saccharomycete is grape wine fruit wine special yeast RW.The grape wine fruit wine special yeast
RW can be purchased from Angel Yeast Co., Ltd, numbering：80000877.
In step (2), the lactic acid bacteria is lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, youth bifid bar
One or several Mixed Microbes in bacterium.
Wherein, lactobacillus bulgaricus can be the numbering from Beijing North Na Chuanlian Bioteknologisk Institut：
The lactobacillus bulgaricus of BNCC223619；Streptococcus thermophilus can be the volume from Beijing North Na Chuanlian Bioteknologisk Institut
Number：The streptococcus thermophilus of BNCC134430；Lactobacillus acidophilus can be the volume from Beijing North Na Chuanlian Bioteknologisk Institut
Number：The lactobacillus acidophilus of BNCC185342；Bifidobacterium adolescentis can be from Beijing North Na Chuanlian Bioteknologisk Institut
Numbering：The bifidobacterium adolescentis of BNCC186535.
In step (3), the method for the freeze-drying is -12~-36 DEG C of low temperature precoolings 12-24 hours, then in vacuum
Dried 24-48 hours under the conditions of degree 20-30pa, 20-30 DEG C of temperature.
In step (4), the condition of the ultramicro grinding is：It is 0~10 DEG C to crush temperature, abrasive media filling rate 60~
70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time 60min.
Present invention also offers the dendrobium candidum ferment Ultramicro-powder that preceding method is prepared.
Antioxidant drug, food or health food are being prepared present invention also offers foregoing dendrobium candidum ferment Ultramicro-powder
In purposes.
The dendrobium candidum ferment Ultramicro-powder mouthfeel that the inventive method is prepared is excellent, and antioxygenic property is also significantly greater than iron
Skin stem of noble dendrobium medicinal material, application prospect is excellent.
Obviously, the above of the invention, according to the ordinary technical knowledge and customary means of this area, is not departing from
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification of other diversified forms can also be made, is replaced or is changed.
The specific embodiment of form, remakes further specifically to the above of the invention by the following examples
It is bright.But this scope for being interpreted as above-mentioned theme of the invention should not be only limitted to following example.It is all based on the above of the present invention
The technology realized belongs to the scope of the present invention.
Specific embodiment
The preparation method of the dendrobium candidum ferment Ultramicro-powder of the present invention of embodiment 1
First, preparation method
(1) zymotechnique：
Take iron sheet stone Mongolian oak medicinal material (orchid dendrobium candidum Dendrobium officinale Kimum et Migo's
Dry stem) 100g, impurity is removed, clean, 2~4 times of amount sterilized waters are added, to soak 30 minutes, crushing and beating is boiled 10 minutes and is gone out
Bacterium, is cooled to room temperature.
In above-mentioned concoction, by weight 0.05~0.15% add saccharomycete (saccharomycete for using for grape wine really
Wine special yeast RW, stock number：80000877, source：Angel Yeast Co., Ltd), maintain fermentation temperature 20~30
DEG C, ferment 24~72 hours, obtain liquid.
In above-mentioned liquid, by weight 2~5% inoculating lactic acid bacteriums (lactobacillus bulgaricus, numbering：BNCC223619,
Source：Beijing North Na Chuanlian Bioteknologisk Institut), 30~35 DEG C of fermentation temperature is maintained, ferment 12~36 hours.
(2) freeze drying process
The iron sheet stone Mongolian oak slurries after above-mentioned fermentation are taken, in -12~-36 DEG C of low temperature precoolings 12-24 hours, in vacuum 20
~30pa, dries 24~48 hours under the conditions of 20~30 DEG C of temperature, obtains the iron sheet stone Mongolian oak freeze-dried powder that ferments.
(3) ultramicro grinding
By in above-mentioned iron sheet stone Mongolian oak freeze-dried powder to Baily pulverizing mill, ultramicro grinding is carried out by following parameter：It is 0 to crush temperature
~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time
60min, collects Ultramicro-powder, obtains final product dendrobium candidum ferment.
2nd, nature examination
The quality control index detection of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is as follows：
According to measurement result, the qualitative character of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is：Moisture is 1
~3%, total starches content>30%, probiotics viable bacteria powder>1×106, cell-wall breaking ratio reaches>98%, particle diameter is at 0.1~100 μm.
The preparation method of the dendrobium candidum ferment Ultramicro-powder of the present invention of embodiment 2
First, preparation method
(1) zymotechnique：
Take fresh iron sheet stone Mongolian oak (orchid dendrobium candidum Dendrobium officinale Kimum et Migo's
Fresh stem), impurity is removed, clean, 1~2 times of amount sterilized water is added, crushing and beating is boiled sterilizing in 10 minutes, is cooled to room temperature.
In above-mentioned concoction, by weight 0.05~0.15% add saccharomycete (saccharomycete for using for grape wine really
Wine special yeast RW, stock number：80000877, source：Angel Yeast Co., Ltd), maintain fermentation temperature 20~30
DEG C, ferment 24~72 hours, obtain liquid.In above-mentioned liquid, by weight 2~5% inoculating lactic acid bacteriums, (streptococcus thermophilus is compiled
Number：BNCC134430, source：Beijing North Na Chuanlian Bioteknologisk Institut), 30~35 DEG C of fermentation temperature of maintenance, fermentation 12~
36 hours.
(2) freeze drying process
The iron sheet stone Mongolian oak slurries after above-mentioned fermentation are taken, in -12~-36 DEG C of low temperature precoolings 12-24 hours, in vacuum
20-30pa, dries 24-48 hours under the conditions of 20-30 DEG C of temperature, obtains the iron sheet stone Mongolian oak freeze-dried powder that ferments.
(3) ultramicro grinding
By in above-mentioned iron sheet stone Mongolian oak freeze-dried powder to Baily pulverizing mill, ultramicro grinding is carried out by following parameter：It is 0 to crush temperature
~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time
60min, collects Ultramicro-powder, obtains final product dendrobium candidum ferment.
2nd, nature examination
The quality control index detection of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is as follows：
According to measurement result, the qualitative character of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is：Moisture is 1
~3%, total starches content>30%, probiotics viable bacteria powder>1×106, cell-wall breaking ratio reaches>98%, particle diameter is at 0.1~100 μm.
The preparation method of the dendrobium candidum ferment Ultramicro-powder of the present invention of embodiment 3
First, preparation method
(1) zymotechnique：
Take iron sheet stone Mongolian oak medicinal material (orchid dendrobium candidum Dendrobium officinale Kimum et Migo's
Dry stem) 100g, impurity is removed, clean, 2~4 times of amount sterilized waters are added, to soak 30 minutes, crushing and beating is boiled 10 minutes and is gone out
Bacterium, is cooled to room temperature.
Or fresh iron sheet stone Mongolian oak is taken, and impurity is removed, clean, 1~2 times of amount sterilized water is added, crushing and beating boils 10 points
Clock, sterilizing, is cooled to room temperature.
In above-mentioned concoction, by weight 0.05~0.15% add saccharomycete (saccharomycete for using for grape wine really
Wine special yeast RW, stock number：80000877, source：Angel Yeast Co., Ltd), maintain fermentation temperature 20~30
DEG C, ferment 24~72 hours, obtain liquid.In above-mentioned liquid, by weight 2~5% inoculating lactic acid bacteriums (lactobacillus acidophilus and green grass or young crops
The Mixed Microbes of spring Bifidobacterium, lactobacillus acidophilus：Numbering:BNCC185342, source：Beijing North is received and creates connection biotechnology research
Institute；Bifidobacterium adolescentis：BNCC186535, source：Beijing North Na Chuanlian Bioteknologisk Institut), maintenance fermentation temperature 30~
35 DEG C, ferment 12~36 hours.
(2) freeze drying process
The iron sheet stone Mongolian oak slurries after above-mentioned fermentation are taken, in -12~-36 DEG C of low temperature precoolings 12-24 hours, in vacuum
20-30pa, dries 24-48 hours under the conditions of 20-30 DEG C of temperature, obtains the iron sheet stone Mongolian oak freeze-dried powder that ferments.
(3) ultramicro grinding
By in above-mentioned iron sheet stone Mongolian oak freeze-dried powder to Baily pulverizing mill, ultramicro grinding is carried out by following parameter：It is 0 to crush temperature
~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time
60min, collects Ultramicro-powder, obtains final product dendrobium candidum ferment.
2nd, nature examination
The quality control index detection of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is as follows：
According to measurement result, the qualitative character of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is：Moisture is 1
~3%, total starches content>30%, probiotics viable bacteria powder>1×106, cell-wall breaking ratio reaches>98%, particle diameter is at 0.1~100 μm.
The following iron sheet stone Mongolian oak ferment product prepared with it by iron sheet stone Mongolian oak medicinal material relatively more used in the present invention
(prepared by embodiment 1) and comparing prove beneficial effects of the present invention：
1st, composition and product sense is compared
Result understands that the obtained iron sheet stone Mongolian oak ferment of the present invention is in good taste, and polyoses content substantially increases.Moisture is low,
Beneficial to preservation.
2nd, antioxidation activity compares
Reference Ge Ruihong, Chu Rui is friendly, Li Jingquan, waits longan ferments to prepare and its Study of Antioxidation, food science and technology,
2015,40 (8)：The method of 262-267 is tested.
Compared with traditional medicine, oxidation resistance is remarkably reinforced the present invention.
To sum up, the inventive method has prepared iron sheet stone Mongolian oak ferment Ultramicro-powder, and its mouthfeel is excellent, and antioxidation is strong,
Traditional dendrobium candidum medicinal material is significantly better than, can be used to prepare mouthfeel and the excellent medicine of antioxygenic property, food or health care food
Product, application prospect is excellent.
Claims (10)
1. a kind of preparation method of dendrobium candidum ferment Ultramicro-powder, it is characterised in that：Step is as follows：
(1) iron sheet stone Mongolian oak is taken, 1~4 times of amount water is added, is soaked 10~30 minutes, crushing and beating, sterilizing obtains concoction；
(2) in step (1) gained concoction, saccharomycete is added by weight 0.05~0.15%, is 20~30 DEG C in temperature
Under the conditions of ferment 24~72 hours, obtain liquid；Again in liquid, lactic acid bacteria is added by weight 2~5%, be then in temperature
Fermented 12~36 hours under conditions of 30~35 DEG C, obtain slurries；
(3) step (2) gained slurries, freeze-drying are taken；
(4) ultramicro grinding, you can.
2. preparation method according to claim 1, it is characterised in that：In step (1), the dendrobium candidum is orchid
Dendrobium candidum Dendrobium officinale Kimum et Migo's dries stem or fresh stem.
3. preparation method according to claim 2, it is characterised in that：In step (1), the dendrobium candidum is orchid
When drying stem of dendrobium candidum Dendrobium officinale Kimum et Migo, adds 2~4 times of water of amount；The iron
When the skin stem of noble dendrobium is the fresh stem of orchid dendrobium candidum Dendrobium officinale Kimum et Migo, addition 1~
2 times of water of amount.
4. preparation method according to claim 1, it is characterised in that：In step (1), the mode of the sterilizing is to boil 10
Minute.
5. preparation method according to claim 1 or 5, it is characterised in that：In step (2), the saccharomycete is grape
Wine fruit wine special yeast RW.
6. preparation method according to claim 1, it is characterised in that：In step (2), the lactic acid bacteria is bulgarian milk
One or several Mixed Microbes in bacillus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis.
7. preparation method according to claim 1, it is characterised in that：In step (3), the method for the freeze-drying is-
12~-36 DEG C of low temperature precoolings 12-24 hours, then to dry 24-48 under the conditions of vacuum 20-30pa, 20-30 DEG C of temperature small
When.
8. preparation method according to claim 1, it is characterised in that：In step (4), the condition of the ultramicro grinding is：
It is 0~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude to crush temperature
5mm, grinding time 60min.
9. the dendrobium candidum ferment Ultramicro-powder for being prepared according to claim 1~8 any one methods described.
10. the dendrobium candidum ferment Ultramicro-powder described in claim 9 is in antioxidant drug, food or health food is prepared
Purposes.
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| CN107751691A (en) * | 2017-10-13 | 2018-03-06 | 河南省三禾药业有限公司 | A kind of stem of noble dendrobium health beverages and preparation method thereof |
| CN108056469A (en) * | 2017-11-28 | 2018-05-22 | 齐鲁工业大学 | The preparation method of seaweed ferment with function of blood sugar reduction |
| CN108077908A (en) * | 2017-11-28 | 2018-05-29 | 昆明理工大学 | A kind of preparation method and applications of the notoginseng haulm ferment of heat clearing and inflammation relieving |
| CN108157945A (en) * | 2017-12-21 | 2018-06-15 | 贵州大学 | The preparation method and application of dendrobium candidum ferment |
| CN108402175A (en) * | 2018-02-11 | 2018-08-17 | 韶关学院 | A kind of dendrobium candidum health care yoghourt and preparation method thereof |
| CN109527315A (en) * | 2018-12-25 | 2019-03-29 | 保山市365生态产业研究所 | With the purple dendrobium ferment drink and preparation method thereof for improving immunity function |
| CN109691670A (en) * | 2019-01-25 | 2019-04-30 | 华南农业大学 | A kind of preparation method and applications of dendrobium candidum enzyme liquid |
| CN112426392A (en) * | 2020-11-05 | 2021-03-02 | 泉后(广州)生物科技研究院有限公司 | Preparation method of dendrobium officinale extract |
| CN112891283A (en) * | 2021-02-01 | 2021-06-04 | 浙江万里学院 | Dendrobium officinale fermentation extract, skin care product and preparation method thereof |
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| CN107245424A (en) * | 2017-08-12 | 2017-10-13 | 谢金藩 | A kind of dendrobium candidum wine preparing process |
| CN107647392A (en) * | 2017-09-27 | 2018-02-02 | 霍山县长冲中药材开发有限公司 | A kind of fermentation noble dendrobium stem distillate of fast purification cholesterol |
| CN107751691A (en) * | 2017-10-13 | 2018-03-06 | 河南省三禾药业有限公司 | A kind of stem of noble dendrobium health beverages and preparation method thereof |
| CN108056469A (en) * | 2017-11-28 | 2018-05-22 | 齐鲁工业大学 | The preparation method of seaweed ferment with function of blood sugar reduction |
| CN108077908A (en) * | 2017-11-28 | 2018-05-29 | 昆明理工大学 | A kind of preparation method and applications of the notoginseng haulm ferment of heat clearing and inflammation relieving |
| CN108157945A (en) * | 2017-12-21 | 2018-06-15 | 贵州大学 | The preparation method and application of dendrobium candidum ferment |
| CN108402175A (en) * | 2018-02-11 | 2018-08-17 | 韶关学院 | A kind of dendrobium candidum health care yoghourt and preparation method thereof |
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| CN109527315A (en) * | 2018-12-25 | 2019-03-29 | 保山市365生态产业研究所 | With the purple dendrobium ferment drink and preparation method thereof for improving immunity function |
| CN109691670A (en) * | 2019-01-25 | 2019-04-30 | 华南农业大学 | A kind of preparation method and applications of dendrobium candidum enzyme liquid |
| CN112426392A (en) * | 2020-11-05 | 2021-03-02 | 泉后(广州)生物科技研究院有限公司 | Preparation method of dendrobium officinale extract |
| CN112891283A (en) * | 2021-02-01 | 2021-06-04 | 浙江万里学院 | Dendrobium officinale fermentation extract, skin care product and preparation method thereof |
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Application publication date: 20170613 |