CN106822601A - A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof - Google Patents

A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof Download PDF

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CN106822601A
CN106822601A CN201710067067.0A CN201710067067A CN106822601A CN 106822601 A CN106822601 A CN 106822601A CN 201710067067 A CN201710067067 A CN 201710067067A CN 106822601 A CN106822601 A CN 106822601A
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dendrobium candidum
ultramicro
ferment
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powder
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刘敬
赵斌
何炜静
张娜
李晓璐
王琼
李水清
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Zhongshan Torch Polytechnic
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/898Orchidaceae (Orchid family)
    • A61K36/8984Dendrobium
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/0012Galenical forms characterised by the site of application
    • A61K9/0053Mouth and digestive tract, i.e. intraoral and peroral administration
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/14Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10Preparation or pretreatment of starting material
    • A61K2236/19Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/31Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/51Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
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Abstract

The invention discloses a kind of preparation method of dendrobium candidum ferment Ultramicro-powder, step is as follows:(1) iron sheet stone Mongolian oak is taken, 1~4 times of amount water is added, is soaked 10~30 minutes, crushing and beating, sterilizing obtains concoction;(2) in step (1) gained concoction, saccharomycete is added by weight 0.05~0.15%, is fermented 24~72 hours under conditions of being 20~30 DEG C in temperature, obtain liquid;Again in liquid, lactic acid bacteria is added by weight 2~5%, fermented 12~36 hours under conditions of being then 30~35 DEG C in temperature, obtain slurries;(3) step (2) gained slurries, freeze-drying are taken;(4) ultramicro grinding, you can.Dendrobium candidum ferment Ultramicro-powder prepared the invention also discloses preceding method and application thereof.The dendrobium candidum ferment Ultramicro-powder mouthfeel that the inventive method is prepared is excellent, and antioxygenic property is also significantly greater than dendrobium candidum medicinal material, and application prospect is excellent.

Description

A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof
Technical field
The present invention relates to a kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof.
Background technology
Dendrobium candidum medicinal material is dry for orchid dendrobium candidum Dendrobium officinale Kimum et Migo's Dry stem, its nature and flavor are sweet, are slightly cold.Return helmet, kidney channel, reinforcing stomach reg fluid, nourishing Yin and clearing heat.For pyreticosis Tianjin wound, dry polydipsia, stomach-Yin is not Foot, deficiency of food is retched, and abnormal heat is not moved back after being ill, fire excess from yin deficiency, and hectic fever due to yin labor heat, mesh is secretly failed to understand, muscles and bones impotence.
At present, dendrobium candidum is normally used as medicinal material and directly uses, and mouthfeel is not good, and drug effect also needs further to be carried It is high.
The content of the invention
The invention provides a kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof.
Present invention firstly provides the preparation method of dendrobium candidum ferment Ultramicro-powder, step is as follows:
(1) iron sheet stone Mongolian oak is taken, 1~4 times of amount water is added, is soaked 10~30 minutes, crushing and beating, sterilizing obtains concoction;
(2) in step (1) gained concoction, saccharomycete is added by weight 0.05~0.15%, is 20~30 in temperature Fermented 24~72 hours under conditions of DEG C, obtain liquid;Again in liquid, lactic acid bacteria is added by weight 2~5%, then in temperature Spend to be fermented 12~36 hours under conditions of 30~35 DEG C, obtain slurries;
(3) step (2) gained slurries, freeze-drying are taken;
(4) ultramicro grinding, you can.
In step (1), the dendrobium candidum is orchid dendrobium candidum Dendrobium officinale Kimum Et Migo's dries stem or fresh stem.
In step (1), the dendrobium candidum is orchid dendrobium candidum Dendrobium officinale Kimum When drying stem of et Migo, adds 2~4 times of water of amount;The dendrobium candidum is orchid dendrobium candidum Dendrobium During the fresh stem of officinale Kimum et Migo, 1~2 times of water of amount is added.
In step (1), the mode of the sterilizing is to boil 10 minutes.
In step (2), the saccharomycete is grape wine fruit wine special yeast RW.The grape wine fruit wine special yeast RW can be purchased from Angel Yeast Co., Ltd, numbering:80000877.
In step (2), the lactic acid bacteria is lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, youth bifid bar One or several Mixed Microbes in bacterium.
Wherein, lactobacillus bulgaricus can be the numbering from Beijing North Na Chuanlian Bioteknologisk Institut: The lactobacillus bulgaricus of BNCC223619;Streptococcus thermophilus can be the volume from Beijing North Na Chuanlian Bioteknologisk Institut Number:The streptococcus thermophilus of BNCC134430;Lactobacillus acidophilus can be the volume from Beijing North Na Chuanlian Bioteknologisk Institut Number:The lactobacillus acidophilus of BNCC185342;Bifidobacterium adolescentis can be from Beijing North Na Chuanlian Bioteknologisk Institut Numbering:The bifidobacterium adolescentis of BNCC186535.
In step (3), the method for the freeze-drying is -12~-36 DEG C of low temperature precoolings 12-24 hours, then in vacuum Dried 24-48 hours under the conditions of degree 20-30pa, 20-30 DEG C of temperature.
In step (4), the condition of the ultramicro grinding is:It is 0~10 DEG C to crush temperature, abrasive media filling rate 60~ 70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time 60min.
Present invention also offers the dendrobium candidum ferment Ultramicro-powder that preceding method is prepared.
Antioxidant drug, food or health food are being prepared present invention also offers foregoing dendrobium candidum ferment Ultramicro-powder In purposes.
The dendrobium candidum ferment Ultramicro-powder mouthfeel that the inventive method is prepared is excellent, and antioxygenic property is also significantly greater than iron Skin stem of noble dendrobium medicinal material, application prospect is excellent.
Obviously, the above of the invention, according to the ordinary technical knowledge and customary means of this area, is not departing from Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification of other diversified forms can also be made, is replaced or is changed.
The specific embodiment of form, remakes further specifically to the above of the invention by the following examples It is bright.But this scope for being interpreted as above-mentioned theme of the invention should not be only limitted to following example.It is all based on the above of the present invention The technology realized belongs to the scope of the present invention.
Specific embodiment
The preparation method of the dendrobium candidum ferment Ultramicro-powder of the present invention of embodiment 1
First, preparation method
(1) zymotechnique:
Take iron sheet stone Mongolian oak medicinal material (orchid dendrobium candidum Dendrobium officinale Kimum et Migo's Dry stem) 100g, impurity is removed, clean, 2~4 times of amount sterilized waters are added, to soak 30 minutes, crushing and beating is boiled 10 minutes and is gone out Bacterium, is cooled to room temperature.
In above-mentioned concoction, by weight 0.05~0.15% add saccharomycete (saccharomycete for using for grape wine really Wine special yeast RW, stock number:80000877, source:Angel Yeast Co., Ltd), maintain fermentation temperature 20~30 DEG C, ferment 24~72 hours, obtain liquid.
In above-mentioned liquid, by weight 2~5% inoculating lactic acid bacteriums (lactobacillus bulgaricus, numbering:BNCC223619, Source:Beijing North Na Chuanlian Bioteknologisk Institut), 30~35 DEG C of fermentation temperature is maintained, ferment 12~36 hours.
(2) freeze drying process
The iron sheet stone Mongolian oak slurries after above-mentioned fermentation are taken, in -12~-36 DEG C of low temperature precoolings 12-24 hours, in vacuum 20 ~30pa, dries 24~48 hours under the conditions of 20~30 DEG C of temperature, obtains the iron sheet stone Mongolian oak freeze-dried powder that ferments.
(3) ultramicro grinding
By in above-mentioned iron sheet stone Mongolian oak freeze-dried powder to Baily pulverizing mill, ultramicro grinding is carried out by following parameter:It is 0 to crush temperature ~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time 60min, collects Ultramicro-powder, obtains final product dendrobium candidum ferment.
2nd, nature examination
The quality control index detection of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is as follows:
According to measurement result, the qualitative character of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is:Moisture is 1 ~3%, total starches content>30%, probiotics viable bacteria powder>1×106, cell-wall breaking ratio reaches>98%, particle diameter is at 0.1~100 μm.
The preparation method of the dendrobium candidum ferment Ultramicro-powder of the present invention of embodiment 2
First, preparation method
(1) zymotechnique:
Take fresh iron sheet stone Mongolian oak (orchid dendrobium candidum Dendrobium officinale Kimum et Migo's Fresh stem), impurity is removed, clean, 1~2 times of amount sterilized water is added, crushing and beating is boiled sterilizing in 10 minutes, is cooled to room temperature.
In above-mentioned concoction, by weight 0.05~0.15% add saccharomycete (saccharomycete for using for grape wine really Wine special yeast RW, stock number:80000877, source:Angel Yeast Co., Ltd), maintain fermentation temperature 20~30 DEG C, ferment 24~72 hours, obtain liquid.In above-mentioned liquid, by weight 2~5% inoculating lactic acid bacteriums, (streptococcus thermophilus is compiled Number:BNCC134430, source:Beijing North Na Chuanlian Bioteknologisk Institut), 30~35 DEG C of fermentation temperature of maintenance, fermentation 12~ 36 hours.
(2) freeze drying process
The iron sheet stone Mongolian oak slurries after above-mentioned fermentation are taken, in -12~-36 DEG C of low temperature precoolings 12-24 hours, in vacuum 20-30pa, dries 24-48 hours under the conditions of 20-30 DEG C of temperature, obtains the iron sheet stone Mongolian oak freeze-dried powder that ferments.
(3) ultramicro grinding
By in above-mentioned iron sheet stone Mongolian oak freeze-dried powder to Baily pulverizing mill, ultramicro grinding is carried out by following parameter:It is 0 to crush temperature ~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time 60min, collects Ultramicro-powder, obtains final product dendrobium candidum ferment.
2nd, nature examination
The quality control index detection of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is as follows:
According to measurement result, the qualitative character of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is:Moisture is 1 ~3%, total starches content>30%, probiotics viable bacteria powder>1×106, cell-wall breaking ratio reaches>98%, particle diameter is at 0.1~100 μm.
The preparation method of the dendrobium candidum ferment Ultramicro-powder of the present invention of embodiment 3
First, preparation method
(1) zymotechnique:
Take iron sheet stone Mongolian oak medicinal material (orchid dendrobium candidum Dendrobium officinale Kimum et Migo's Dry stem) 100g, impurity is removed, clean, 2~4 times of amount sterilized waters are added, to soak 30 minutes, crushing and beating is boiled 10 minutes and is gone out Bacterium, is cooled to room temperature.
Or fresh iron sheet stone Mongolian oak is taken, and impurity is removed, clean, 1~2 times of amount sterilized water is added, crushing and beating boils 10 points Clock, sterilizing, is cooled to room temperature.
In above-mentioned concoction, by weight 0.05~0.15% add saccharomycete (saccharomycete for using for grape wine really Wine special yeast RW, stock number:80000877, source:Angel Yeast Co., Ltd), maintain fermentation temperature 20~30 DEG C, ferment 24~72 hours, obtain liquid.In above-mentioned liquid, by weight 2~5% inoculating lactic acid bacteriums (lactobacillus acidophilus and green grass or young crops The Mixed Microbes of spring Bifidobacterium, lactobacillus acidophilus:Numbering:BNCC185342, source:Beijing North is received and creates connection biotechnology research Institute;Bifidobacterium adolescentis:BNCC186535, source:Beijing North Na Chuanlian Bioteknologisk Institut), maintenance fermentation temperature 30~ 35 DEG C, ferment 12~36 hours.
(2) freeze drying process
The iron sheet stone Mongolian oak slurries after above-mentioned fermentation are taken, in -12~-36 DEG C of low temperature precoolings 12-24 hours, in vacuum 20-30pa, dries 24-48 hours under the conditions of 20-30 DEG C of temperature, obtains the iron sheet stone Mongolian oak freeze-dried powder that ferments.
(3) ultramicro grinding
By in above-mentioned iron sheet stone Mongolian oak freeze-dried powder to Baily pulverizing mill, ultramicro grinding is carried out by following parameter:It is 0 to crush temperature ~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude 5mm, grinding time 60min, collects Ultramicro-powder, obtains final product dendrobium candidum ferment.
2nd, nature examination
The quality control index detection of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is as follows:
According to measurement result, the qualitative character of the iron sheet stone Mongolian oak ferment Ultramicro-powder obtained by the present invention is:Moisture is 1 ~3%, total starches content>30%, probiotics viable bacteria powder>1×106, cell-wall breaking ratio reaches>98%, particle diameter is at 0.1~100 μm.
The following iron sheet stone Mongolian oak ferment product prepared with it by iron sheet stone Mongolian oak medicinal material relatively more used in the present invention (prepared by embodiment 1) and comparing prove beneficial effects of the present invention:
1st, composition and product sense is compared
Result understands that the obtained iron sheet stone Mongolian oak ferment of the present invention is in good taste, and polyoses content substantially increases.Moisture is low, Beneficial to preservation.
2nd, antioxidation activity compares
Reference Ge Ruihong, Chu Rui is friendly, Li Jingquan, waits longan ferments to prepare and its Study of Antioxidation, food science and technology, 2015,40 (8):The method of 262-267 is tested.
Compared with traditional medicine, oxidation resistance is remarkably reinforced the present invention.
To sum up, the inventive method has prepared iron sheet stone Mongolian oak ferment Ultramicro-powder, and its mouthfeel is excellent, and antioxidation is strong, Traditional dendrobium candidum medicinal material is significantly better than, can be used to prepare mouthfeel and the excellent medicine of antioxygenic property, food or health care food Product, application prospect is excellent.

Claims (10)

1. a kind of preparation method of dendrobium candidum ferment Ultramicro-powder, it is characterised in that:Step is as follows:
(1) iron sheet stone Mongolian oak is taken, 1~4 times of amount water is added, is soaked 10~30 minutes, crushing and beating, sterilizing obtains concoction;
(2) in step (1) gained concoction, saccharomycete is added by weight 0.05~0.15%, is 20~30 DEG C in temperature Under the conditions of ferment 24~72 hours, obtain liquid;Again in liquid, lactic acid bacteria is added by weight 2~5%, be then in temperature Fermented 12~36 hours under conditions of 30~35 DEG C, obtain slurries;
(3) step (2) gained slurries, freeze-drying are taken;
(4) ultramicro grinding, you can.
2. preparation method according to claim 1, it is characterised in that:In step (1), the dendrobium candidum is orchid Dendrobium candidum Dendrobium officinale Kimum et Migo's dries stem or fresh stem.
3. preparation method according to claim 2, it is characterised in that:In step (1), the dendrobium candidum is orchid When drying stem of dendrobium candidum Dendrobium officinale Kimum et Migo, adds 2~4 times of water of amount;The iron When the skin stem of noble dendrobium is the fresh stem of orchid dendrobium candidum Dendrobium officinale Kimum et Migo, addition 1~ 2 times of water of amount.
4. preparation method according to claim 1, it is characterised in that:In step (1), the mode of the sterilizing is to boil 10 Minute.
5. preparation method according to claim 1 or 5, it is characterised in that:In step (2), the saccharomycete is grape Wine fruit wine special yeast RW.
6. preparation method according to claim 1, it is characterised in that:In step (2), the lactic acid bacteria is bulgarian milk One or several Mixed Microbes in bacillus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis.
7. preparation method according to claim 1, it is characterised in that:In step (3), the method for the freeze-drying is- 12~-36 DEG C of low temperature precoolings 12-24 hours, then to dry 24-48 under the conditions of vacuum 20-30pa, 20-30 DEG C of temperature small When.
8. preparation method according to claim 1, it is characterised in that:In step (4), the condition of the ultramicro grinding is: It is 0~10 DEG C, abrasive media filling rate 60~70%, vibration frequency 1000 times/min, oscillation intensity 8g, amplitude to crush temperature 5mm, grinding time 60min.
9. the dendrobium candidum ferment Ultramicro-powder for being prepared according to claim 1~8 any one methods described.
10. the dendrobium candidum ferment Ultramicro-powder described in claim 9 is in antioxidant drug, food or health food is prepared Purposes.
CN201710067067.0A 2017-02-07 2017-02-07 A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof Pending CN106822601A (en)

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CN107245424A (en) * 2017-08-12 2017-10-13 谢金藩 A kind of dendrobium candidum wine preparing process
CN107647392A (en) * 2017-09-27 2018-02-02 霍山县长冲中药材开发有限公司 A kind of fermentation noble dendrobium stem distillate of fast purification cholesterol
CN107751691A (en) * 2017-10-13 2018-03-06 河南省三禾药业有限公司 A kind of stem of noble dendrobium health beverages and preparation method thereof
CN108056469A (en) * 2017-11-28 2018-05-22 齐鲁工业大学 The preparation method of seaweed ferment with function of blood sugar reduction
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CN109527315A (en) * 2018-12-25 2019-03-29 保山市365生态产业研究所 With the purple dendrobium ferment drink and preparation method thereof for improving immunity function
CN109691670A (en) * 2019-01-25 2019-04-30 华南农业大学 A kind of preparation method and applications of dendrobium candidum enzyme liquid
CN112426392A (en) * 2020-11-05 2021-03-02 泉后(广州)生物科技研究院有限公司 Preparation method of dendrobium officinale extract
CN112891283A (en) * 2021-02-01 2021-06-04 浙江万里学院 Dendrobium officinale fermentation extract, skin care product and preparation method thereof

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* Cited by examiner, † Cited by third party
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CN107245424A (en) * 2017-08-12 2017-10-13 谢金藩 A kind of dendrobium candidum wine preparing process
CN107647392A (en) * 2017-09-27 2018-02-02 霍山县长冲中药材开发有限公司 A kind of fermentation noble dendrobium stem distillate of fast purification cholesterol
CN107751691A (en) * 2017-10-13 2018-03-06 河南省三禾药业有限公司 A kind of stem of noble dendrobium health beverages and preparation method thereof
CN108056469A (en) * 2017-11-28 2018-05-22 齐鲁工业大学 The preparation method of seaweed ferment with function of blood sugar reduction
CN108077908A (en) * 2017-11-28 2018-05-29 昆明理工大学 A kind of preparation method and applications of the notoginseng haulm ferment of heat clearing and inflammation relieving
CN108157945A (en) * 2017-12-21 2018-06-15 贵州大学 The preparation method and application of dendrobium candidum ferment
CN108402175A (en) * 2018-02-11 2018-08-17 韶关学院 A kind of dendrobium candidum health care yoghourt and preparation method thereof
CN108402175B (en) * 2018-02-11 2022-02-18 韶关学院 Dendrobium officinale health-care yoghourt and preparation method thereof
CN109527315A (en) * 2018-12-25 2019-03-29 保山市365生态产业研究所 With the purple dendrobium ferment drink and preparation method thereof for improving immunity function
CN109691670A (en) * 2019-01-25 2019-04-30 华南农业大学 A kind of preparation method and applications of dendrobium candidum enzyme liquid
CN112426392A (en) * 2020-11-05 2021-03-02 泉后(广州)生物科技研究院有限公司 Preparation method of dendrobium officinale extract
CN112891283A (en) * 2021-02-01 2021-06-04 浙江万里学院 Dendrobium officinale fermentation extract, skin care product and preparation method thereof

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Application publication date: 20170613