CN112426392A - Preparation method of dendrobium officinale extract - Google Patents
Preparation method of dendrobium officinale extract Download PDFInfo
- Publication number
- CN112426392A CN112426392A CN202011224885.5A CN202011224885A CN112426392A CN 112426392 A CN112426392 A CN 112426392A CN 202011224885 A CN202011224885 A CN 202011224885A CN 112426392 A CN112426392 A CN 112426392A
- Authority
- CN
- China
- Prior art keywords
- dendrobium officinale
- fermentation
- extract
- preparation
- dendrobium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to a preparation method of a dendrobium officinale extract. According to the method, by a double-bacterium secondary fermentation method, firstly, enzyme generated in the fermentation process of lactic acid bacteria is utilized to decompose cellulose and crack plant cell walls to release active substances, then, saccharomycetes are inoculated, and the nutrient elements or macromolecules in the plant cells are used as substrates to decompose and synthesize functional substances such as polyphenol flavone and the like beneficial to skin care, so that the content of effective components such as phenols, polysaccharides, amino acids and the like can be obviously increased, and the obtained dendrobium extract has high oxidation resistance and moisturizing effects; moreover, the method has the advantages of short flow, simple operation and high yield, and is suitable for large-scale industrial production.
Description
Technical Field
The invention belongs to the technical field of cosmetics. More particularly, relates to a preparation method of a dendrobium officinale extract.
Background
Dendrobium officinale, also known as "black clover", is a plant of the genus Dendrobium of the family Orchidaceae, a perennial epiphytic herb. Since ancient times, dendrobium officinale has always been a famous and precious traditional Chinese medicine in China, has the reputation of gold in medicine, and is listed as the first of the Jiudao celestial grass in the Taoisesheng from the Taoisk. The dendrobium officinale contains various active ingredients such as dendrobium polysaccharides, flavonoids, phenols, amino acids, vitamins and the like, has the effects of resisting oxidation and aging, treating certain diseases and the like, and is often used as an effective ingredient of medicines, health-care products and cosmetics. Among them, in the catalogue of names of used cosmetic raw materials (2015 edition), published by the State food and drug administration, the dendrobium officinale extract is listed as a cosmetic raw material, and various cosmetics using the dendrobium officinale as a main functional raw material are increasing, so that the dendrobium officinale extract has a wide application value and market prospect in the field of cosmetics.
In order to fully utilize active ingredients in the dendrobium officinale, and to enable the effect to reach the optimum, the prior art adopts various methods to extract the effective ingredients of the dendrobium officinale. The common extraction methods comprise aqueous solution heating extraction, organic solvent extraction and the like, but the aqueous solution heating extraction needs to be carried out at high temperature, active ingredients are easy to damage, and the organic solvent extraction solution has organic solvent residues and certain potential safety hazard. Chinese patent application CN111334540A discloses a method for extracting dendrobium candidum polysaccharide by biological fermentation, which is characterized in that after being fermented by saccharomycetes, dendrobium candidum is separated and purified to prepare the dendrobium candidum polysaccharide, the yield, the protein clearance rate and the decolorization rate are high, but the effects of moisture retention, oxidation resistance and the like are general, and the requirements of consumers on the performance of cosmetics at present can not be met.
Therefore, a preparation method for obtaining the dendrobium officinale extract with high yield of effective components and good moisturizing and antioxidant performances is urgently needed.
Disclosure of Invention
The invention aims to solve the technical problems of overcoming the defects and shortcomings of low yield of effective components and general moisturizing and antioxidant performance of the dendrobium officinale extract obtained in the prior art, and provides a preparation method of the dendrobium officinale extract with high yield of the effective components and good moisturizing and antioxidant performance.
The above purpose of the invention is realized by the following technical scheme:
a preparation method of a dendrobium officinale extract is characterized by comprising the following steps:
s1, drying and crushing the dendrobium officinale, adding the crushed dendrobium officinale into a fermentation container, adding water and a carbon source, and sterilizing to obtain a dendrobium officinale culture solution;
s2, inoculating lactic acid bacteria into the dendrobium officinale culture solution obtained in the step S1, and stirring and culturing at 30-40 ℃ to obtain fermentation liquor;
s3, inoculating yeast into the fermentation liquor obtained in the step S2, stirring and fermenting at 25-35 ℃, sterilizing after fermentation, centrifuging, and filtering supernatant to obtain filtrate, namely the dendrobium officinale extract.
Further, in the step S1, the addition amount of the dendrobium officinale is 10-50 g/L.
Further, in step S1, the carbon source is selected from one or more of fructose, sucrose and lactose.
Further, in step S1, the amount of the carbon source added is 0.5 to 5%.
Preferably, in the step S1, the volume of the water is 30-35L.
Furthermore, in step S1, the Dendrobium officinale is dried at 50-80 ℃ for 1-10 h.
Further, in step S1, the dendrobium officinale is crushed and then screened by a screen of 50-200 meshes.
Further, in step S2, the lactic acid bacteria are lactobacillus plantarum or streptococcus thermophilus. Preferably, the lactobacillus is activated in an MRS culture medium for 12-48 hours before inoculation.
Further, in step S2, the inoculation amount of the lactic acid bacteria is 1 to 5%.
Furthermore, in step S2, the stirring culture time is 12-48 h. Preferably, the rotation speed of the stirring culture is 100-400 rpm.
Further, in step S3, the yeast is brewer 'S yeast or baker' S yeast. Preferably, the yeast is activated in a YPD culture medium for 12-48 hours before inoculation.
Further, in step S3, the yeast is inoculated in an amount of 1-5%.
Further, in the step S3, the stirring fermentation time is 12-48 h. Preferably, the rotation speed of the stirring fermentation is 100-400 rpm.
Further, in step S3, the sterilization condition is 115-125 ℃ high temperature sterilization for 10-20 min.
Further, in step S3, the centrifugation is carried out at 4000-14000 rpm for 10-30 min.
Furthermore, in step S3, the pore size of the filter membrane for filtration is 0.2-5 μm. Preferably, the filter membrane is a ceramic membrane.
Further, in the steps S2 and S3, the aeration rate during the agitation culture or the agitation fermentation is 0 to 2.0 vvm. Preferably, the ventilation amount is 0vvm when the fermentation strain is Streptococcus thermophilus, and 0.5-2.0 vvm when the fermentation strain is other strains.
The dendrobium officinale extract prepared by the invention can be applied to the preparation of antioxidant and/or skin care products.
The invention has the following beneficial effects:
according to the preparation method of the dendrobium officinale extract, by adopting a double-bacterium secondary fermentation method, firstly, enzymes generated in the fermentation process of lactic acid bacteria are utilized to decompose cellulose and crack plant cell walls to release active substances, then, saccharomycetes are inoculated to decompose and synthesize functional substances beneficial to skin care, such as polyphenol flavone and the like by taking nutrient elements or macromolecules in the plant cells as substrates, so that the contents of effective components, such as phenols, polysaccharides, amino acids and the like, can be remarkably increased, and the obtained dendrobium extract has high oxidation resistance and moisturizing effects; moreover, the method has the advantages of short flow, simple operation and high yield, and is suitable for large-scale industrial production.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 preparation method of dendrobium officinale extract
The preparation method of the dendrobium officinale extract comprises the following steps:
s1, beating the dry dendrobium officinale strips into small sections with the length less than 2cm by using a stirrer, then putting the small sections of the dendrobium officinale into an oven, drying for 3 hours at 65 ℃, adding the small sections of the dendrobium officinale into an ultrafine grinder, grinding into powder, sieving by using a 100-mesh sieve, adding the obtained dendrobium officinale powder into a fermentation tank (50L) filled with 30L of water according to the ratio of 10g/L, adding 0.5% of cane sugar, and sterilizing to obtain a dendrobium officinale culture solution;
s2, inoculating streptococcus thermophilus into the MRS culture medium, activating for 12h, inoculating into the dendrobium officinale culture solution obtained in the step S1, wherein the inoculation amount is 1%, and stirring and culturing are carried out for 12h under the conditions of 30 ℃, the rotation speed of 200rpm and the ventilation volume of 0vvm, so as to obtain fermentation liquor;
s3, inoculating saccharomyces cerevisiae in a YPD culture medium for activation for 12h, inoculating the activated saccharomyces cerevisiae in the fermentation liquid obtained in the step S2, wherein the inoculation amount is 1%, stirring and fermenting at 25 ℃, 300rpm and an air flow of 0.5vvm for 12h, sterilizing at 115 ℃ for 10min after the fermentation is finished, centrifuging at 4000rpm for 10min, taking supernatant, filtering by using a 0.2-micron ceramic membrane, and collecting filtrate, namely the dendrobium officinale extract.
Embodiment 2 preparation method of dendrobium officinale extract
The preparation method of the dendrobium officinale extract comprises the following steps:
s1, beating the dry dendrobium officinale strips into small sections with the length less than 2cm by using a stirrer, then putting the small sections of the dendrobium officinale into an oven, drying for 3 hours at 65 ℃, adding the small sections of the dendrobium officinale into an ultrafine grinder, grinding into powder, sieving by using a 100-mesh sieve, adding the obtained dendrobium officinale powder into a fermentation tank (50L) filled with 35L of water according to 50g/L, adding 5% of cane sugar, and sterilizing to obtain a dendrobium officinale culture solution;
s2, inoculating streptococcus thermophilus into an MRS culture medium, activating for 48 hours, inoculating into the dendrobium officinale culture solution obtained in the step S1, wherein the inoculation amount is 5%, and stirring and culturing for 48 hours at 40 ℃, the rotation speed of 200rpm and the ventilation volume of 0vvm to obtain fermentation liquor;
s3, inoculating saccharomyces cerevisiae in a YPD culture medium for activation for 48h, inoculating the activated saccharomyces cerevisiae in the fermentation liquid obtained in the step S2, wherein the inoculation amount is 5%, stirring and fermenting at 35 ℃, 300rpm and 2vvm of ventilation for 48h, sterilizing at 125 ℃ for 20min after the fermentation is finished, centrifuging at 13000rpm for 30min, taking supernatant, filtering with a 5-micron ceramic membrane, and collecting filtrate, namely the dendrobium officinale extract.
Embodiment 3 preparation method of dendrobium officinale extract
The preparation method of the dendrobium officinale extract comprises the following steps:
s1, beating the dry dendrobium officinale strips into small sections with the length less than 2cm by using a stirrer, then putting the small sections of the dendrobium officinale into a drying oven, drying for 3 hours at 65 ℃, adding the small sections of the dendrobium officinale into an ultrafine grinder, grinding into powder, sieving by using a 100-mesh sieve, adding the obtained dendrobium officinale powder into a 35L water fermentation tank (50L) according to the ratio of 30g/L, adding 2% of cane sugar, and sterilizing to obtain a dendrobium officinale culture solution;
s2, inoculating streptococcus thermophilus into an MRS culture medium, activating for 24 hours, inoculating into the dendrobium officinale culture solution obtained in the step S1, wherein the inoculation amount is 4%, and stirring and culturing are carried out for 24 hours at 37 ℃, the rotation speed is 200rpm, and the ventilation volume is 0vvm, so as to obtain a fermentation liquid;
s3, inoculating saccharomyces cerevisiae in a YPD culture medium for activation for 24 hours, inoculating the activated saccharomyces cerevisiae in the fermentation liquid obtained in the step S2, wherein the inoculation amount is 2%, stirring and fermenting the saccharomyces cerevisiae at 28 ℃, 300rpm and an air flow of 1.5vvm for 24 hours, sterilizing the fermentation liquid at 121 ℃ for 15min at high temperature after the fermentation is finished, centrifuging the fermentation liquid for 15min at 10000rpm, taking supernate, filtering the supernate with a 0.8-micron ceramic membrane, and collecting filtrate, namely the dendrobium officinale extract.
Preparation method of dendrobium officinale extract in examples and comparative examples
The difference from example 3 is that examples 4 to 8 and comparative examples 1 to 7 change the kinds and addition amounts of the carbon source, lactic acid bacteria and yeast in steps S1 to S3, and the rest parameters and operations refer to example 3. See table 1 for specific changes.
TABLE 1 examples and comparative examples differ in the type and amount of carbon source, lactic acid bacteria and yeast
| Group of | Dendrobium officinale (g/L) | Carbon source (%) | Lactic acid bacterium (%) | Yeast (%) |
| Example 1 | 10 | 0.5% sucrose | 1% Streptococcus thermophilus | 1% beer yeast |
| Example 2 | 50 | 5% sucrose | 5% Streptococcus thermophilus | 5% beer yeast |
| Example 3 | 30 | 2% sucrose | 4% Streptococcus thermophilus | 2% Saccharomyces cerevisiae |
| Example 4 | 30 | 2% sucrose | 4% Streptococcus thermophilus | 2% baker's yeast |
| Example 5 | 30 | 2% sucrose | 4% Lactobacillus plantarum | 2% Saccharomyces cerevisiae |
| Example 6 | 30 | 2% sucrose | 4% Lactobacillus plantarum | 2% baker's yeast |
| Example 7 | 30 | 2% of fructose | 4% Lactobacillus plantarum | 2% Saccharomyces cerevisiae |
| Example 8 | 30 | 2% lactose | 4% Lactobacillus plantarum | 2% Saccharomyces cerevisiae |
| Comparative example 1 | 30 | 2% sucrose | 6% Lactobacillus plantarum | / |
| Comparative example 2 | 30 | 2% sucrose | / | 6% beer yeast |
| Comparative example 3 | 30 | 2% sucrose | 4％Schizophyllum commune (Fr.) Quel | 2% Saccharomyces cerevisiae |
| Comparative example 4 | 30 | 2% sucrose | 4% Bacillus subtilis | 2% Saccharomyces cerevisiae |
| Comparative example 5 | 30 | 2% sucrose | 4% Lactobacillus bulgaricus | 2% Saccharomyces cerevisiae |
| Comparative example 6 | 30 | 2% sucrose | 4% Bifidobacterium | 2% Saccharomyces cerevisiae |
| Comparative example 7 | 30 | 2% sucrose | 4% Lactobacillus plantarum | 2% of Aspergillus oryzae |
Comparative example 8 preparation method of dendrobium officinale extract
Referring to the method of example 1 of chinese patent application CN111334540A, the prepared dendrobium officinale polysaccharide is prepared into a 3% aqueous solution.
Experimental example 1 content and yield of effective components of Dendrobium officinale extract
The content of effective components and the yield of polysaccharide of the dendrobium officinale extract obtained in the examples and the comparative examples are determined, and the results are shown in table 2. Note: the polysaccharide yield is the percentage of the polysaccharide content in the extract in the total polysaccharide content in the fermentation solution before fermentation.
TABLE 2 concentration and yield of the effective components of Dendrobium officinale extract
As can be seen from table 2, the dendrobium officinale extract prepared in embodiments 1-8 of the present invention has high content of active ingredients such as total phenols, polysaccharides, amino acids, etc., and the polysaccharide yield is also high; compared with the comparative examples 1-7, the content of active ingredients and the yield of polysaccharide are both obviously reduced after the extraction method is changed; comparative example 8 was prepared with purified polysaccharide and had lower total phenol and amino acid content.
Experimental example 2 antioxidant Activity of Dendrobium officinale extract
The antioxidant activity of the extracts of Dendrobium officinale obtained in examples and comparative examples was determined, wherein DPPH radical clearance was measured using 30mg/ml of the starting material and hydroxyl radical clearance was measured as such, and the results are shown in Table 3.
TABLE 3 antioxidant Activity of Dendrobium officinale extract
As can be seen from table 3, the DPPH radical clearance rate and the hydroxyl radical clearance rate of the dendrobium officinale extract prepared in the embodiment of the present invention are both at a high level, and have significant antioxidant activity; in contrast, the comparative example, in which the extraction method was changed, the antioxidant activity was significantly reduced.
Experimental example 3 moisturizing effect of Dendrobium officinale extract
70 healthy and skin disease-free volunteers aged 20-40 years are selected to test the moisturizing effect of the dendrobium officinale extract. The test samples are the dendrobium officinale extracting solutions obtained in the example 2, the example 5, the example 8, the comparative example 1, the comparative example 4, the comparative example 7 and the comparative example 8, the test environment temperature of each group of 10 people is 24-26 ℃, and the humidity is 40% -60%; the test instrument is a CM825 model skin oil-water acid-base tester; testing the inner 2 x 5cm area of the arm; see table 4 for results.
TABLE 4 moisturizing effect of Dendrobium officinale extract
| Group of | Before use | 30min after use | 3h after use | 6h after use |
| Example 2 | 31.0 | 45.8 | 42.6 | 37.9 |
| Example 5 | 31.2 | 46.6 | 43.9 | 40.6 |
| Example 8 | 31.5 | 43.8 | 40.3 | 37.0 |
| Comparative example 1 | 31.0 | 38.5 | 35.7 | 32.9 |
| Comparative example 4 | 31.4 | 41.3 | 37.3 | 34.0 |
| Comparative example 7 | 31.4 | 40.8 | 38.0 | 33.4 |
| Comparative example 8 | 31.8 | 41.5 | 38.1 | 35.5 |
As can be seen from table 4, the dendrobium officinale extract prepared in the embodiment of the invention has a good moisturizing effect, can significantly improve the moisture of the skin, can maintain a high moisture content in the skin 6 hours after use, and has a significant moisturizing effect; in the comparative example, the moisturizing effect is remarkably reduced after the extraction method is changed.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A preparation method of a dendrobium officinale extract is characterized by comprising the following steps:
s1, drying and crushing the dendrobium officinale, adding the crushed dendrobium officinale into a fermentation container, adding water and a carbon source, and sterilizing to obtain a dendrobium officinale culture solution;
s2, inoculating lactic acid bacteria into the dendrobium officinale culture solution obtained in the step S1, and stirring and culturing at 30-40 ℃ to obtain fermentation liquor;
s3, inoculating yeast into the fermentation liquor obtained in the step S2, stirring and fermenting at 25-35 ℃, sterilizing after fermentation, centrifuging, and filtering supernatant to obtain filtrate, namely the dendrobium officinale extract.
2. The preparation method according to claim 1, wherein in step S1, the addition amount of Dendrobium officinale is 10-50 g/L.
3. The method according to claim 1, wherein in step S1, the carbon source is one or more selected from fructose, sucrose and lactose.
4. The method according to claim 1, wherein the carbon source is added in an amount of 0.5 to 5% in step S1.
5. The method according to claim 1, wherein the lactic acid bacteria is Lactobacillus plantarum or Streptococcus thermophilus in step S2.
6. The method according to claim 1, wherein the amount of the lactic acid bacteria inoculated in step S2 is 1-5%.
7. The method according to claim 1, wherein the yeast is Saccharomyces cerevisiae or Saccharomyces cerevisiae in step S3.
8. The method according to claim 1, wherein the yeast is inoculated in an amount of 1 to 5% in step S3.
9. The method according to claim 1, wherein in step S3, the pore size of the filter membrane for filtration is 0.2-5 μm.
10. The method according to claim 1, wherein the aeration rate during the agitation culture or the agitation fermentation is 0 to 2.0vvm in steps S2 and S3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011224885.5A CN112426392A (en) | 2020-11-05 | 2020-11-05 | Preparation method of dendrobium officinale extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011224885.5A CN112426392A (en) | 2020-11-05 | 2020-11-05 | Preparation method of dendrobium officinale extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112426392A true CN112426392A (en) | 2021-03-02 |
Family
ID=74695484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011224885.5A Pending CN112426392A (en) | 2020-11-05 | 2020-11-05 | Preparation method of dendrobium officinale extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112426392A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113398045A (en) * | 2021-06-25 | 2021-09-17 | 广州环亚化妆品科技有限公司 | Anti-aging and relieving aloe fermented product and preparation method and application thereof |
| CN113425662A (en) * | 2021-08-16 | 2021-09-24 | 湖州伊婷生物科技有限公司 | Hyaluronic acid and dendrobium officinale yeast fermentation product composition and cosmetic |
| CN113440465A (en) * | 2021-08-23 | 2021-09-28 | 云南英格生物技术有限公司 | Rice fermentation product, rice fermentation extract, preparation method and application |
| CN113576991A (en) * | 2021-08-06 | 2021-11-02 | 广东药科大学 | Preparation method of dendrobium officinale fermentation liquor |
| CN114304335A (en) * | 2021-12-21 | 2022-04-12 | 昆明生物制造研究院有限公司 | Method for enriching active ingredients of dendrobium leaves through fermentation and application of method |
| CN114854518A (en) * | 2022-06-23 | 2022-08-05 | 湖州师范学院 | Cardamine hupingshanesis selenium-rich yellow wine and production method thereof |
| CN115364025A (en) * | 2022-08-26 | 2022-11-22 | 珠海市丝域生物科技有限公司 | Composition with scalp care effect and preparation method and application thereof |
| CN116509783A (en) * | 2023-05-18 | 2023-08-01 | 肽源（广州）生物科技有限公司 | Dendrobium officinale extract capable of promoting collagen synthesis and preparation method and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585762A (en) * | 2015-01-19 | 2015-05-06 | 中国食品发酵工业研究院 | Dendrobium officinale fermented product and preparation method thereof |
| CN105146509A (en) * | 2015-07-28 | 2015-12-16 | 昆明宝成生物科技有限公司 | Novel probiotic dendrobium enzyme and production technology thereof |
| CN105707628A (en) * | 2016-03-11 | 2016-06-29 | 三株福尔制药有限公司 | Probiotic fermentation dendrobium officinale protocorm composition and preparation method thereof |
| CN106616974A (en) * | 2016-11-14 | 2017-05-10 | 霍山三宝生物保健品有限公司 | Production method for dendrobe enzyme capable of promoting immunity |
| CN106822601A (en) * | 2017-02-07 | 2017-06-13 | 中山火炬职业技术学院 | A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof |
| CN107629965A (en) * | 2017-09-25 | 2018-01-26 | 云南中医学院 | A kind of preparation method of dendrobium candidum fungi fermentation product and its fermented product and application |
| CN108402175A (en) * | 2018-02-11 | 2018-08-17 | 韶关学院 | A kind of dendrobium candidum health care yoghourt and preparation method thereof |
| CN108450556A (en) * | 2018-04-10 | 2018-08-28 | 南京工业大学 | A kind of yoghourt-flavored fermentation dendrobe powder and preparation method thereof |
| CN109527315A (en) * | 2018-12-25 | 2019-03-29 | 保山市365生态产业研究所 | With the purple dendrobium ferment drink and preparation method thereof for improving immunity function |
| CN109691670A (en) * | 2019-01-25 | 2019-04-30 | 华南农业大学 | A kind of preparation method and applications of dendrobium candidum enzyme liquid |
-
2020
- 2020-11-05 CN CN202011224885.5A patent/CN112426392A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585762A (en) * | 2015-01-19 | 2015-05-06 | 中国食品发酵工业研究院 | Dendrobium officinale fermented product and preparation method thereof |
| CN105146509A (en) * | 2015-07-28 | 2015-12-16 | 昆明宝成生物科技有限公司 | Novel probiotic dendrobium enzyme and production technology thereof |
| CN105707628A (en) * | 2016-03-11 | 2016-06-29 | 三株福尔制药有限公司 | Probiotic fermentation dendrobium officinale protocorm composition and preparation method thereof |
| CN106616974A (en) * | 2016-11-14 | 2017-05-10 | 霍山三宝生物保健品有限公司 | Production method for dendrobe enzyme capable of promoting immunity |
| CN106822601A (en) * | 2017-02-07 | 2017-06-13 | 中山火炬职业技术学院 | A kind of dendrobium candidum ferment Ultramicro-powder and preparation method thereof |
| CN107629965A (en) * | 2017-09-25 | 2018-01-26 | 云南中医学院 | A kind of preparation method of dendrobium candidum fungi fermentation product and its fermented product and application |
| CN108402175A (en) * | 2018-02-11 | 2018-08-17 | 韶关学院 | A kind of dendrobium candidum health care yoghourt and preparation method thereof |
| CN108450556A (en) * | 2018-04-10 | 2018-08-28 | 南京工业大学 | A kind of yoghourt-flavored fermentation dendrobe powder and preparation method thereof |
| CN109527315A (en) * | 2018-12-25 | 2019-03-29 | 保山市365生态产业研究所 | With the purple dendrobium ferment drink and preparation method thereof for improving immunity function |
| CN109691670A (en) * | 2019-01-25 | 2019-04-30 | 华南农业大学 | A kind of preparation method and applications of dendrobium candidum enzyme liquid |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113398045A (en) * | 2021-06-25 | 2021-09-17 | 广州环亚化妆品科技有限公司 | Anti-aging and relieving aloe fermented product and preparation method and application thereof |
| CN113576991A (en) * | 2021-08-06 | 2021-11-02 | 广东药科大学 | Preparation method of dendrobium officinale fermentation liquor |
| CN113425662A (en) * | 2021-08-16 | 2021-09-24 | 湖州伊婷生物科技有限公司 | Hyaluronic acid and dendrobium officinale yeast fermentation product composition and cosmetic |
| CN113440465A (en) * | 2021-08-23 | 2021-09-28 | 云南英格生物技术有限公司 | Rice fermentation product, rice fermentation extract, preparation method and application |
| CN114304335A (en) * | 2021-12-21 | 2022-04-12 | 昆明生物制造研究院有限公司 | Method for enriching active ingredients of dendrobium leaves through fermentation and application of method |
| CN114304335B (en) * | 2021-12-21 | 2024-02-20 | 昆明生物制造研究院有限公司 | Method for fermenting and enriching active ingredients of dendrobium leaves and application of method |
| CN114854518A (en) * | 2022-06-23 | 2022-08-05 | 湖州师范学院 | Cardamine hupingshanesis selenium-rich yellow wine and production method thereof |
| CN114854518B (en) * | 2022-06-23 | 2023-10-27 | 湖州师范学院 | Selenium-enriched yellow wine containing cardamine hupingshanensis and production method thereof |
| CN115364025A (en) * | 2022-08-26 | 2022-11-22 | 珠海市丝域生物科技有限公司 | Composition with scalp care effect and preparation method and application thereof |
| CN116509783A (en) * | 2023-05-18 | 2023-08-01 | 肽源（广州）生物科技有限公司 | Dendrobium officinale extract capable of promoting collagen synthesis and preparation method and application thereof |
| CN116509783B (en) * | 2023-05-18 | 2024-03-15 | 肽源（广州）生物科技有限公司 | Dendrobium officinale extract capable of promoting collagen synthesis and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112426392A (en) | Preparation method of dendrobium officinale extract | |
| CN109717340B (en) | Fermentation preparation method of two-step cordyceps militaris enzyme combined with composite enzymolysis | |
| CN111317694B (en) | Eucommia ulmoides fermentation extracting solution, preparation method thereof and application thereof in cosmetics | |
| CN108823261B (en) | Ultralow-molecular-weight dendrobium officinale polysaccharide and preparation and application thereof | |
| KR101370386B1 (en) | Preparation method of fermented red ginseng using effective micro-organisms | |
| JP3160560B2 (en) | Ginseng vinegar and foods containing it | |
| CN103271951B (en) | Method for preparing cordyceps preparations with high adenosine contents | |
| CN107629965A (en) | A kind of preparation method of dendrobium candidum fungi fermentation product and its fermented product and application | |
| CN113143812B (en) | Preparation method of kava pepper fermentation product, kava pepper fermentation product and application of kava pepper fermentation product in cosmetics | |
| CN115572738A (en) | Dendrobium officinale fermentation liquor with anti-inflammatory, relieving and repairing effects as well as preparation method and application thereof | |
| CN114304335A (en) | Method for enriching active ingredients of dendrobium leaves through fermentation and application of method | |
| CN114557927B (en) | Yeast essence water composition, yeast essence water, and preparation method and application thereof | |
| CN111135123B (en) | Preparation method and application of selaginella tamariscina fermentation extracting solution | |
| KR101564487B1 (en) | Manufacturing method of small molecule Ginsenoside | |
| KR101372400B1 (en) | A manufacturing method of red ginseng staf4h with enhanced trace ginsenoside | |
| CN116376731B (en) | Application of Wilkham yeast in preparing Prinsepia utilis extract | |
| CN111349678A (en) | Extraction method of rape pollen polysaccharide and extraction product | |
| CN109275819A (en) | A kind of Ganoderma lucidum submerged fermentation technology and its fermented product and application | |
| KR20160126591A (en) | Method of producing ginseng fermented extract, Ginseng fermented extract produced by the same and Health functional foods comprising the same | |
| CN114209621B (en) | Moisturizing and antioxidant red yeast rice fermentation product and preparation method and application thereof | |
| CN108669483A (en) | A kind of sargassum fermentation product and its liquid fermentation preparation process and application | |
| CN113332387B (en) | Corn silk fermentation method | |
| CN114410421A (en) | Preparation method of roxburgh rose and black glutinous rice vinegar | |
| KR102218224B1 (en) | Cosmetic composition for preventing or improving skin inflammation or skin aging comprising fermented product of Ligularia stenocephala leaf as an active ingredient | |
| CN113648262A (en) | Lily leaf and cherry fermented product for cosmetic and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210302 |
|
| RJ01 | Rejection of invention patent application after publication |