CN107629965A - A kind of preparation method of dendrobium candidum fungi fermentation product and its fermented product and application - Google Patents
A kind of preparation method of dendrobium candidum fungi fermentation product and its fermented product and application Download PDFInfo
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Abstract
The present invention discloses preparation method and its fermented product and the application of a kind of dendrobium candidum fungi fermentation product.The preparation method of the dendrobium candidum fungi fermentation product includes stem of noble dendrobium preparation, actication of culture, liquid seeds culture, stem of noble dendrobium fungi fermentation, fermented product processing step.Preparation method of the fermented product through dendrobium candidum fungi fermentation product is prepared.The application is that prepared by dendrobium candidum fungi fermentation product into food, health food, cosmetics and/or medicine.The present invention is fermented with the medical edible fungal with materials such as cellulases to dendrobium candidum, dendrobium fiber element etc. is decomposed into small molecule carbon source, retain the beneficiating ingredient of dendrobium candidum again, and fermented product mellow in taste, have dendrobium candidum delicate fragrance and fungi unique perfume concurrently, also containing abundant fungi polysaccharide, saponins, Polyphenols, flavonoids isoreactivity material, active ingredient is easy to intestinal absorption, has and improves immunity, antitumor, anti-oxidant, reducing blood lipid and blood pressure, regulation gut flora balance and other effects.
Description
Technical field
The invention belongs to technical field of bioengineering, and in particular to one kind have improve immunity, it is antitumor, anti-oxidant,
The preparation method and its fermented product for the dendrobium candidum fungi fermentation product that reducing blood lipid and blood pressure, regulation gut flora balance are with answering
With.
Background technology
Macro fungi is a kind of ancient biology, about 10000~20000 kinds of Chinese large-sized fungi, about 8000 be described
Kind,《Sheng Nong's herbal classic》With《Compendium of Materia Medica》There is the record of fungi medical value.Integration of drinking and medicinal herbs macro fungi refers to one kind
Human body will not be caused to be poisoned after edible, the fungi of the macroscopic fructification of meat or colloid can be formed.Integration of drinking and medicinal herbs it is true
Bacterium is due to rich in terpenoid (sequiterpene, diterpene-kind compound and triterpene compound, tetraterpenes compound), polysaccharide
Compound, polypeptide, alkaloid, phytosterin compound, nucleoside compound isoreactivity material, mostly with antibacterial, it is antiviral,
Anticancer, anti-oxidant, raising immunity, hypotensive, hypoglycemic, norcholesterol and other effects, are that current fungus class most has development and application
One kind of prospect.Due to being restricted by physiological status particularity and complexity and external environment condition, the fungi of integration of drinking and medicinal herbs is caused
It is rare that fructification is formed in nature, particularly being formed can be needed for many years with fructification.And artificial cultivation is extremely difficult, bar is cultivated
Part is harsh, and growth cycle is up to, it is difficult to meets the market needs increasingly expanded.There is now a variety of macro fungis can pass through liquid
Fermentation, substantial amounts of mycelium is obtained within the short time (such as 5~45d), and its a variety of medicinal ingredient is included in zymotic fluid.
Dendrobium candidum (Dendrobium officinale Kimura et Migo) is orchid family (Orchidaceae) stem of noble dendrobium
Belong to (Dendrobium) perennial herbaceous plant that grows nonparasitically upon another plant.Modern traditional Chinese medicine research shows：Contain a large amount of polysaccharide and more in stem of Dendrobium
The pharmaceutical compositions such as kind alkaloid, there is nourishing Yin and moistening lung, beneficial stomach kidney tonifying, tonifying five zang organs consumptive disease, removing heat from the lung to relieve cough, brain tonic, improving eyesight, resist
Fatigue, body immunity is improved, anti-aging, suppresses the effect such as tumour, expansion blood vessel, is the most rare in Dendrobium medicinal plant
Rare kind.Modern pharmacological research shows, dendrobium candidum has anti-oxidant, and antifatigue, enhancing is immune, promotees digestion, hypoglycemic, drop
Blood pressure and other effects.
In the prior art, the extracting method of stem of noble dendrobium active material is based on water extraction and alcohol precipitation method, also Enzymatic Extraction and ultrasound
The methods of ripple assisted extraction.In recent years, using Chinese medicine as the research of medicinal or edible fungus fermentation substrate and using increasingly
More, the property of medicine matrix of active composition is fermented by medicinal fungi, and it can provide nutrition raised growth thalline needed for fungi, together
When and can change the structural constituent of its original because of the enzyme of fungi.During addition is appropriate using Chinese medicine as culture medium or in the medium
Medicine, some compositions in Chinese medicine may be converted, improve its drug effect.Its principle is to be turned using the powerful decomposition of the enzyme of fungi
Change ability, the fiber of Chinese medicine in culture medium, the material such as carbohydrate and protein can be not only used, and in metabolic process
In the composition of some in Chinese medicine can be converted, so as to improve active ingredient of Chinese herbs.
Grifola frondosus (Grifolarondosa (Dicks.：Fr.) S.F.Gray) it is integration of drinking and medicinal herbs Tan bacterium, category basidiomycetes is sub-
Door, Hymenomycetes, Aphyllophorales, Polyporaceae, set flower Pseudomonas.Rich in the functional components such as polysaccharide, vitamin, trace element, energy
Prevent leucoderma, anaemia, scurvy, while have antitumor, hypoglycemic and other effects, modern medicine further proves, moreover it is possible to be anti-
Only artery sclerosis, raising immunity, anti-ageing a variety of effects of waiting for a long time.
Dome ganoderma lucidum is also known as white sesame (Fuscoporiapunctata (Fr.) Cum), also known as birch stripping pore bacterium, Polyporaceae,
Piptoporus, belong to the obligate domestomycetes of Betula trees, cause xylem formed brown rot, be distributed in China Heilungkiang, Jilin,
The areas such as Liaoning, the Inner Mongol, there is important practical value and medical value.
Red ganoderma lucidum (Ganoderma lucidium) is a kind of fungi of integration of drinking and medicinal herbs, the traditional Chinese medical science is regarded to strengthen the body resistance to consolidate the constitution,
Strengthen immunologic function and improve Abwehrkraft des Koepers, there is hypoglycemic, hypotensive, neurasthenia is antitumor, protecting liver and detoxication, resists
Aging and other effects.
Rainbow conk or Corilus versicolor Quel. (CorilusversicolorQuel.), are subordinate to Basidiomycotina, Hymenomycetes, non-pleat
Zoopagales, Polyporaceae, rainbow conk category or Coriolus Qu61.Rainbow conk is widely used in controlling for the diseases such as cancer and immune deficiency in China
Treat, be considered as god's medicine of curing the disease by among the people.Research shows that coriolan has significant antioxidation activity, free to superoxide anion
Base and DPPH free radicals have significant scavenging action.
Hedgehog hydnum (Hericium erinaceus (RullexF.) Pers.) is integration of drinking and medicinal herbs Tan bacterium, has antiulcer, anti-inflammatory
Disease, antitumor, anti-aging, antifatigue, raising body's hypoxia tolerance, increase myocardial blood output quantity, acceleration body blood follow
The effect such as ring, hypoglycemic, liver protecting and reducing blood lipid, hypotensive.
Poria cocos (Poria cocos) is under the jurisdiction of Eumycota, is integration of drinking and medicinal herbs Tan bacterium.Poria cocos is On Polyporaceae Poria cocos
Dry sclerotia, there is the effect such as diuresis, calmness, antitumor, the anti-dirty tissue of protection, the enhancing rhythm of the heart.
The patent of Application No. 2015100256084 discloses a kind of dendrobium candidum fermented product and preparation method thereof, will
Extracting in water after dendrobium candidum is broken, extract solution is made, then accesses Leuconostoc mesenteroides and fermented, the first fermentation is made
Liquid；Pectase is added into the fruits and vegetables liquid of pH4.5~6 simultaneously to be digested, and fruits and vegetables enzymolysis liquid is made, and into fruits and vegetables enzymolysis liquid
Access lactic acid bacteria is fermented, and the second zymotic fluid is made；Then mulriple yeasts fermentation is accessed into the second zymotic fluid, is made the
Three zymotic fluids；Finally the first zymotic fluid and the 3rd zymotic fluid are mixed and centrifuged, to supernatant homogeneous, sterilizing is dendrobium candidum
Fermented product.
The patent of Application No. 201610422473 discloses a kind of highly dissoluble probiotics fermention dendrobe powder and its preparation
With application, it carries out enzymolysis processing to the stem of noble dendrobium using cellulase before fermentation, improves stem of noble dendrobium dissolubility and palatability, simultaneously
It is molten to solve existing probiotics fermention dendrobe powder but also as the substrate of probiotics fermention for glucide caused by cellulase hydrolysis
Solution property is poor, brews the shortcomings that rear easily layering, mouthfeel unpolished rice.
The content of the invention
The first object of the present invention is to provide a kind of simple method, process safety and is not subject to seasonal restrictions, and has and improves
The system for the dendrobium candidum fungi fermentation product that immunity, antitumor, anti-oxidant, reducing blood lipid and blood pressure, regulation gut flora balance
Preparation Method；Second purpose is to provide the fermented product that a kind of first mesh is prepared；3rd purpose is to provide one kind second
The application of the fermented product of purpose.
What the first object of the present invention was realized in：Including stem of noble dendrobium preparation, actication of culture, liquid seeds culture, the stem of noble dendrobium
Fungi fermentation, fermented product processing step, are comprised the following steps that：
A, prepared by the stem of noble dendrobium：The fresh bar of dendrobium candidum is removed into inedible position and cleaned, segment is cut after naturally dry, is beaten
It is standby to obtain stem of noble dendrobium slurry；
B, actication of culture：The integration of drinking and medicinal herbs fungi strain of preservation is transferred on inclined-plane solid medium at 28~30 DEG C
Cultivate 5~10d；
C, liquid seeds culture：Above-mentioned activated integration of drinking and medicinal herbs fungi strain is cut into one piece to be inoculated in equipped with 100mL
In the 300mL triangular flasks of fluid nutrient medium, with 140~150r/min, 7~10d of concussion and cultivate under the conditions of 28~30 DEG C, until
Form macroscopic mycelium and/or bacterium ball is covered with triangular flask and terminates to ferment；
D, stem of noble dendrobium fungi fermentation：By the stem of noble dendrobium being prepared slurry with fermented nutritive liquid by 2:8~3:7 material liquid volume is than mixed
Conjunction is inserted 100mL in 300mL triangular flasks and sterilized, and the cultured fungi liquid seeds of step C are pressed into mixed liquor 10 after sterilizing
~20% volume mass ratio is inoculated into mixed liquor, and ferment at constant temperature is until forming macroscopic mycelium and/or bacterium ball cloth
Full triangular flask terminates to ferment；
E, fermented product is handled：The stem of noble dendrobium fungal fermented filtrate of D step fermentation ends is centrifuged through 1000r/min and removes thalline
With stem of noble dendrobium slag, zymotic fluid is taken to obtain dendrobium candidum fungi fermentation product through pasteurization.
What the second object of the present invention was realized in：What the preparation method of dendrobium candidum fungi fermentation product was prepared
Dendrobium candidum fungi fermentation product.
What the third object of the present invention was realized in：Dendrobium candidum fungi fermentation product prepare food, health food,
Application in cosmetics and/or medicine.
The present invention has the advantages that compared with prior art：
1) present invention uses fungi grifola frondosus, Poria cocos, hedgehog hydnum, dome ganoderma lucidum, rainbow conk or the red ganoderma lucidum of integration of drinking and medicinal herbs to iron
Skin dendrobium fresh strips carry out cold fermentation, have the powerful enzyme system of the materials such as decomposition of cellulose using fungi, with fungi to iron sheet stone
The fresh bar of dry measure used in former times carries out submerged fermentation and is decomposed into small molecule carbon source, adds the utilization rate of dendrobium candidum material effective component, not only gram
The overweight herbaceous taste that the stem of noble dendrobium has is taken, and can retains the beneficiating ingredients such as water-soluble polysaccharide in dendrobium candidum, dendrobine, simultaneously
Polysaccharide in the stem of noble dendrobium etc. may additionally facilitate the growth of fungi and the production of metabolite so that fermented product dissolubility is good, color and luster is bright
Bright uniform, mellow in taste, free from extraneous odour, have dendrobium candidum delicate fragrance and fungi unique perfume, excellent taste concurrently.
2) present invention is with the fungi fermentation dendrobium candidum of integration of drinking and medicinal herbs, and metabolite caused by itself is such as in growth for fungi
Fungi polysaccharide, alkaloid, nucleoside compound etc. accumulate, and there is these compounds antibacterial, antiviral, raising immunity etc. to make
With due to dendrobium candidum and the metabolite double action of fungi so that the dendrobium candidum fungi fermentation product of preparation contains rich
The bioactive substances such as rich fungi polysaccharide, saponins, Polyphenols, flavonoids, dendrobium candidum fungi fermentation product, which has, to be improved
Immunity, antitumor, anti-oxidant, reducing blood lipid and blood pressure, regulation gut flora balance and other effects, available for preparing food, health care
Food, cosmetics and/or medicine, have a extensive future.
3) present invention is using the medical edible fungal fermentation dendrobium candidum with cellulase, without adding the exotics such as enzyme
Matter, production cost is not only saved, and simplify production stage；By medium culture and the method for liquid fermentation, bacterium is harvested
Silk and macroscopic fructification obtain the medicinal active ingredient of fungi so that preparation method can realize industrialization, standardization
Production and it is not subject to seasonal restrictions, not only the cycle is short, it is low and small by external environment influence to save labour, cost, and can fill
Code insurance demonstrate,proves the steady quality of fermented product.
4) the dendrobium candidum fungi fermentation product instant for preparing of the present invention, any addition is not added in preparation process
Agent, relatively conventional water extraction and alcohol precipitation method, Enzymatic Extraction and ultrasonic assistant extraction, avoids the introducing of organic solvent, not only makes
Standby process safety environmental protection, and fermented product is green and healthy.
Brief description of the drawings
Fig. 1 is the determination oxidative knot for the dendrobium candidum fungi fermentation product that embodiment is prepared with reference examples
Fruit；
In figure：DPPH -1,1- diphenyl -2- trinitrophenyl-hydrazines, alias 1,1- diphenyl -2- hardships diazanyls (free radical)；
OH-hydroxyl radical free radical；Reducing power-go back protohydrogen, including NADH (reduced Coenzyme II), NADPH (reduced coenzyme Ⅰ), FADH
(flavin adenine dinucleotide (FAD)).
Embodiment
The present invention is further illustrated with reference to the accompanying drawings and examples, but the present invention is not subject in any way
Limitation, based on present invention teach that any changes and modifications made, belong to protection scope of the present invention.
The preparation method of the dendrobium candidum fungi fermentation product of the present invention includes stem of noble dendrobium preparation, actication of culture, liquid seeds
Culture, stem of noble dendrobium fungi fermentation, fermented product processing step, are comprised the following steps that：
A, prepared by the stem of noble dendrobium：The fresh bar of dendrobium candidum is removed into inedible position and cleaned, segment is cut after naturally dry, is beaten
It is standby to obtain stem of noble dendrobium slurry；
B, actication of culture：The integration of drinking and medicinal herbs fungi strain of preservation is transferred on inclined-plane solid medium at 28~30 DEG C
Cultivate 5~10d；
C, liquid seeds culture：Above-mentioned activated integration of drinking and medicinal herbs fungi strain is cut into one piece to be inoculated in equipped with 100mL
In the 300mL triangular flasks of fluid nutrient medium, with 140~150r/min, 7~10d of concussion and cultivate under the conditions of 28~30 DEG C, until
Form macroscopic mycelium and/or bacterium ball is covered with triangular flask and terminates to ferment；
D, stem of noble dendrobium fungi fermentation：By the stem of noble dendrobium being prepared slurry with fermented nutritive liquid by 2:8~3:7 material liquid volume is than mixed
Conjunction is inserted 100mL in 300mL triangular flasks and sterilized, and the cultured fungi liquid seeds of step C are pressed into mixed liquor 10 after sterilizing
~20% volume mass ratio is inoculated into mixed liquor, and ferment at constant temperature is until forming macroscopic mycelium and/or bacterium ball cloth
Full triangular flask terminates to ferment；
E, fermented product is handled：The stem of noble dendrobium fungal fermented filtrate of D step fermentation ends is centrifuged through 1000r/min and removes thalline
With stem of noble dendrobium slag, zymotic fluid is taken to obtain dendrobium candidum fungi fermentation product through pasteurization.
The integration of drinking and medicinal herbs fungi is grifola frondosus, dome ganoderma lucidum, Poria cocos, rainbow conk, red ganoderma lucidum, hedgehog hydnum.
The grifola frondosus is G2M510 bacterial strains (preserving number GIM5.10), G2M563 bacterial strains (GIM5.63), G2M5164 bacterial strains
(GIM5.164), the dome ganoderma lucidum is G2M56 bacterial strains (GIM5.6), the Poria cocos are G2M5219 bacterial strains (GIM5.219), institute
It is G to state rainbow conk2M5178 bacterial strains (GIM5.178), the red ganoderma lucidum are G2M5250 bacterial strains (GIM5.250), the hedgehog hydnum are
G2M5208 bacterial strains (GIM5.208), above-mentioned bacterial strains are all from Guangdong Province's Culture Collection (GDMCC).
The step A is that the fresh bar of fresh dendrobium candidum is removed into inedible part and cleaned, segment after naturally dry, so
Afterwards by material water 1:5~1:10 mass volume ratio mashing, obtain stem of noble dendrobium slurry it is sterilized after to be placed in -20 DEG C of refrigerations standby.
The fresh bar raw material sources of dendrobium candidum in the step A do not do considered critical, can be the fresh iron of different sources
Skin dendrobium fresh strips.
Inclined-plane solid medium in the step B is PDA slant mediums, and the PDA inclined-plane cultures based component presses matter
Amount percentage includes：Potato 20%, glucose 2%, agar 1.5~2%, the water of chloramphenicol 0.01% and surplus.
Strain in the step B is inoculated at 1/3 above the surface bottom of slant medium, and the strain is trained on inclined-plane
Support to cultivate to mycelia at 28~30 DEG C on base and cover with inclined-plane.
Fluid nutrient medium in the step C is PDA liquid medium, and the PDA liquid medium composition presses quality hundred
Ratio is divided to include：Potato 20%, the water of glucose 2%, chloramphenicol 0.01% and surplus.
Fermented nutritive liquid composition in the D steps includes by mass percentage：0.13% peptone, 0.1%
MgSO4, 0.2% KH2PO4And the water of surplus.
The condition of stem of noble dendrobium ferment at constant temperature is in the D steps：Shaken under 28~30 DEG C of constant temperatures with 140~150r/min
Cultivate 3~10d.
Sterilizing in the D steps is that the triangular flask for being loaded into mixed liquor sterilizes under the normal condition of this area, such as
Can be sterilized 20~-30min at 120~125 DEG C.
Added with sweet substances such as honey, sucrose or xylitols in zymotic fluid in the E steps.
The method of the dendrobium candidum fungi fermentation product of the present invention also includes freeze-drying step, the freeze-drying step
It is that the zymotic fluid of E steps below 5%, is crushed to 200 mesh and obtains stem of noble dendrobium fungi fermentation system through vacuum freeze drying to water content
Product pulvis.
The dendrobium candidum fungi fermentation product that the preparation method of the dendrobium candidum fungi fermentation product of the present invention is prepared.
The dendrobium candidum fungi fermentation product of the present invention answering in food, health food, cosmetics and/or medicine is prepared
With.
The oxidation resistance for the dendrobium candidum fungi fermentation product being prepared in various embodiments of the present invention and reference examples is surveyed
It is as follows to determine method：
DPPH Scavenging activities determine：Press《The anti-oxidant spectrum effect relationship research of peppermint medicinal material based on DPPH, FRAP method》, Xu Jing
Crystalline substance etc., Beijing University of Chinese Medicine's journal, 2015,38 (6):405-410 methods describeds are detected；
Hydroxyl radical free radical (OH) Scavenging activity determines：Press《The research of purple carrot juice antioxidation activity in vitro》, the brave spring is gorgeous
Deng China brewages, 2015,34 (1)：85-88 methods describeds are detected；
Reducing power measure is pressed《The changing rule of oxidation resistance in Tea Fungus Fermentation》, Zhu Xiaoqing etc., production and section
Grind experience, 2010,36 (11)：111-114 methods describeds are detected.
Embodiment 1
S100：The fresh bar of fresh dendrobium candidum is removed into inedible part and cleaned, segment after naturally dry, then by material
Water 1:5 (g/mL) mass volume ratio mashing, obtaining stem of noble dendrobium slurry, to be placed in -20 DEG C of refrigerations standby.
S200：By the integration of drinking and medicinal herbs fungi strain grifola frondosus G of preservation2M510 bacterial strains (preserving number GIM5.10) are from parent species inclined-plane
In test tube 1cm is cut with inoculation shovel2The mycelia block of size, it is inoculated at 1/3 above the surface bottom of PDA slant mediums, in
5~10d is cultivated under the conditions of 28 DEG C and covers with inclined-plane to mycelia；PDA inclined-plane culture based components include by mass percentage：Ma Ling
Potato 20%, glucose 2%, agar 2%, the water of chloramphenicol 0.01% and surplus.
S300：The S200 integration of drinking and medicinal herbs fungi strains activated are cut into 1cm with inoculation shovel2Fungus block, be inoculated in and be equipped with
In the 300mL triangular flasks of 100mL PDA liquid mediums, with 150r/min 7~10d of concussion and cultivate under the conditions of 28 DEG C, until
Form macroscopic mycelium and bacterium ball is covered with triangular flask and terminates to ferment；PDA liquid medium composition wraps by mass percentage
Include：Potato 20%, the water of glucose 2%, chloramphenicol 0.01% and surplus.
S400：The stem of noble dendrobium slurry that S100 is prepared is with fermented nutritive liquid by 2:8 (mL/mL) material liquid volume ratio will
100mL mixed liquors are inserted in 300mL triangular flasks, and in 121 DEG C of insulated sterilizing 20min, it is after cooling that S300 is cultured true
Bacteria liquid seed 10g is inoculated into 100mL mixed liquors (by the 10% of mixeding liquid volume), in 28 DEG C of constant temperature 150r/min concussion hairs
3~10d of ferment is covered with triangular flask and terminates to ferment until forming macroscopic mycelium and bacterium ball；Fermented nutritive liquid composition presses quality
Percentage includes：0.13% peptone, 0.1% MgSO4, 0.2% KH2PO4And the water of surplus.
S500：The stem of noble dendrobium fungal fermented filtrate of S400 fermentation ends is taken, is centrifuged through 1000r/min and removes thalline and dendrobium candidum
Slag, zymotic fluid is taken to obtain dendrobium candidum fungi fermentation product through pasteurization.
Embodiment 2
S100：The fresh bar of fresh dendrobium candidum is removed into inedible part and cleaned, segment after naturally dry, then by material
Water 1:10 (g/mL) mass volume ratio mashing, obtaining stem of noble dendrobium slurry, to be placed in -20 DEG C of refrigerations standby.
S200：By the integration of drinking and medicinal herbs fungi strain Poria cocos G of preservation2M5219 bacterial strains (preserving number GIM5.219) are from parent species inclined-plane
In test tube 1cm is cut with inoculation shovel2The mycelia block of size, it is inoculated at 1/3 above the surface bottom of PDA slant mediums, in
5~10d is cultivated under the conditions of 28 DEG C and covers with inclined-plane to mycelia；PDA inclined-plane culture based components include by mass percentage：Ma Ling
Potato 20%, glucose 2%, agar 1.5%, the water of chloramphenicol 0.01% and surplus.
S300：The S200 integration of drinking and medicinal herbs fungi strains activated are cut into 1cm with inoculation shovel2Fungus block, be inoculated in and be equipped with
In the 300mL triangular flasks of 100mL PDA liquid mediums, with 140r/min 7~10d of concussion and cultivate under the conditions of 28 DEG C, until
Form macroscopic mycelium and bacterium ball is covered with triangular flask and terminates to ferment；PDA liquid medium composition wraps by mass percentage
Include：Potato 20%, the water of glucose 2%, chloramphenicol 0.01% and surplus.
S400：The stem of noble dendrobium slurry that S100 is prepared is with fermented nutritive liquid by 3:7 (mL/mL) material liquid volume ratio will
100mL mixed liquors are inserted in 300mL triangular flasks, and in 125 DEG C of insulated sterilizing 20min, it is after cooling that S300 is cultured true
Bacteria liquid seed 20g is inoculated into 100mL mixed liquors (by the 20% of mixeding liquid volume), in 28 DEG C of constant temperature 140r/min concussion hairs
3~10d of ferment is covered with triangular flask and terminates to ferment until forming macroscopic mycelium and bacterium ball；Fermented nutritive liquid composition presses quality
Percentage includes：0.13% peptone, 0.1% MgSO4, 0.2% KH2PO4And the water of surplus.
S500：The stem of noble dendrobium fungal fermented filtrate of S400 fermentation ends is taken, is centrifuged through 1000r/min and removes thalline and dendrobium candidum
Slag, zymotic fluid is taken then to add a certain proportion of honey through pasteurization according to personal taste, obtain dendrobium candidum fungi fermentation
Product.
Embodiment 3
S100：The fresh bar of fresh dendrobium candidum is removed into inedible part and cleaned, segment after naturally dry, then by material
Water 1:15 (g/mL) mass volume ratio mashing, obtaining stem of noble dendrobium slurry, to be placed in -20 DEG C of refrigerations standby.
S200：By the red ganoderma lucidum G of the integration of drinking and medicinal herbs fungi strain of preservation2M5250 bacterial strains (preserving number GIM5.250) are oblique from parent species
Interview Guan Zhongyong inoculation shovels cut 1cm2The mycelia block of size, it is inoculated at 1/3 above the surface bottom of PDA slant mediums,
5~10d is cultivated under the conditions of 30 DEG C and covers with inclined-plane to mycelia；PDA inclined-plane culture based components include by mass percentage：Horse
Bell potato 20%, glucose 2%, agar 2%, the water of chloramphenicol 0.01% and surplus.
S300：The S200 integration of drinking and medicinal herbs fungi strains activated are cut into 1cm with inoculation shovel2Fungus block, be inoculated in and be equipped with
In the 300mL triangular flasks of 100mL PDA liquid mediums, with 150r/min 7~10d of concussion and cultivate under the conditions of 30 DEG C, until
Form macroscopic mycelium and bacterium ball is covered with triangular flask and terminates to ferment；PDA liquid medium composition wraps by mass percentage
Include：Potato 20%, the water of glucose 2%, chloramphenicol 0.01% and surplus.
S400：The stem of noble dendrobium slurry that S100 is prepared is with fermented nutritive liquid by 4:6 (mL/mL) material liquid volume ratio will
100mL mixed liquors are inserted in 300mL triangular flasks, and in 120 DEG C of insulated sterilizing 30min, it is after cooling that S300 is cultured true
Bacteria liquid seed 25g is inoculated into 100mL mixed liquors (by the 25% of mixeding liquid volume), in 30 DEG C of constant temperature 150r/min concussion hairs
3~10d of ferment is covered with triangular flask and terminates to ferment until forming macroscopic mycelium and bacterium ball；Fermented nutritive liquid composition presses quality
Percentage includes：0.13% peptone, 0.1% MgSO4, 0.2% KH2PO4And the water of surplus.
S500：The stem of noble dendrobium fungal fermented filtrate of S400 fermentation ends is taken, is centrifuged through 1000r/min and removes thalline and dendrobium candidum
Slag, zymotic fluid is taken then to add a certain proportion of sucrose through pasteurization according to personal taste, obtain dendrobium candidum fungi fermentation
Product.
Embodiment 4
S100：The fresh bar of fresh dendrobium candidum is removed into inedible part and cleaned, segment after naturally dry, then by material
Water 1:20 (g/mL) mass volume ratio mashing, obtaining stem of noble dendrobium slurry, to be placed in -20 DEG C of refrigerations standby.
S200：By the integration of drinking and medicinal herbs fungi strain hedgehog hydnum G of preservation2M5208 bacterial strains (preserving number GIM5.208) are from parent species inclined-plane
In test tube 1cm is cut with inoculation shovel2The mycelia block of size, it is inoculated at 1/3 above the surface bottom of PDA slant mediums, in
5~10d is cultivated under the conditions of 28 DEG C and covers with inclined-plane to mycelia；PDA inclined-plane culture based components include by mass percentage：Ma Ling
Potato 20%, glucose 2%, agar 1.5%, the water of chloramphenicol 0.01% and surplus.
S300：The S200 integration of drinking and medicinal herbs fungi strains activated are cut into 1cm with inoculation shovel2Fungus block, be inoculated in and be equipped with
In the 300mL triangular flasks of 100mL PDA liquid mediums, with 150r/min 7~10d of concussion and cultivate under the conditions of 28 DEG C, until
Form macroscopic mycelium and bacterium ball is covered with triangular flask and terminates to ferment；PDA liquid medium composition wraps by mass percentage
Include：Potato 20%, the water of glucose 2%, chloramphenicol 0.01% and surplus.
S400：The stem of noble dendrobium slurry that S100 is prepared is with fermented nutritive liquid by 5:5 (mL/mL) material liquid volume ratio will
100mL mixed liquors are inserted in 300mL triangular flasks, and in 120 DEG C of insulated sterilizing 20min, it is after cooling that S300 is cultured true
Bacteria liquid seed 30g is inoculated into 100mL mixed liquors (by the 30% of mixeding liquid volume), in 30 DEG C of constant temperature 150r/min concussion hairs
3~10d of ferment is covered with triangular flask and terminates to ferment until forming macroscopic mycelium and bacterium ball；Fermented nutritive liquid composition presses quality
Percentage includes：0.13% peptone, 0.1% MgSO4, 0.2% KH2PO4And the water of surplus.
S500：The stem of noble dendrobium fungal fermented filtrate of S400 fermentation ends is taken, is centrifuged through 1000r/min and removes thalline and dendrobium candidum
Slag, zymotic fluid is taken then to add a certain proportion of xylitol through pasteurization according to personal taste, obtain dendrobium candidum fungi hair
Ferment product.
Reference examples 1：
S100：The fresh bar of fresh dendrobium candidum is removed into inedible part and cleaned, segment after naturally dry, then by material
Water 1:5 (g/mL) mass volume ratio mashing, obtaining stem of noble dendrobium slurry, to be placed in -20 DEG C of refrigerations standby.
S200：The stem of noble dendrobium slurry that S100 is prepared is with fermented nutritive liquid by 2:8 (mL/mL) material liquid volume ratio will
100mL mixed liquors are inserted in 300mL triangular flasks, and in 121 DEG C of insulated sterilizing 20min, after cooling by sterilized water 10g (by mixed
10%) conjunction liquid product is inoculated into 100mL mixed liquors, in 28 DEG C of 3~10d of constant temperature 150r/min concussion fermentations；It is Nutrious fermented
Liquid composition includes by mass percentage：0.13% peptone, 0.1% MgSO4, 0.2% KH2PO4And the water of surplus.
S300：Take S200 fermentations 10d stem of noble dendrobium fungal fermented filtrate to be centrifuged through 1000r/min and remove dendrobium candidum slag, take hair
Zymotic fluid obtains dendrobium candidum fungi fermentation product through pasteurization.
Reference examples 2：
S100：The fresh bar of fresh dendrobium candidum is removed into inedible part and cleaned, segment after naturally dry, then by material
Water 1:10 (g/mL) mass volume ratio mashing, obtaining stem of noble dendrobium slurry, to be placed in -20 DEG C of refrigerations standby.
S200：The stem of noble dendrobium slurry that S100 is prepared is with fermented nutritive liquid by 3:7 (mL/mL) material liquid volume ratio will
100mL mixed liquors are inserted in 300mL triangular flasks, and in 121 DEG C of insulated sterilizing 20min, after cooling by sterilized water 20g (by mixed
20%) conjunction liquid product is inoculated into 100mL mixed liquors, in 30 DEG C of 3~10d of constant temperature 140r/min concussion fermentations；It is Nutrious fermented
Liquid composition includes by mass percentage：0.13% peptone, 0.1% MgSO4, 0.2% KH2PO4And the water of surplus.
S300：Take S200 fermentations 10d stem of noble dendrobium fungal fermented filtrate to be centrifuged through 1000r/min and remove dendrobium candidum slag, take hair
Zymotic fluid obtains dendrobium candidum fungi fermentation product through pasteurization.
The dendrobium candidum fungi fermentation product that Example 1,2,3 and 4 and reference examples 1,2 obtain carries out subjective appreciation,
And carry out determination oxidative.Dendrobium candidum fungi fermentation product subjective appreciation standard such as table 1, evaluation result such as table 2, resist
Oxidability measurement result such as Fig. 1.
The dendrobium candidum fungi fermentation product subjective appreciation standard of table 1
The dendrobium candidum fungi fermentation product subjective appreciation score of table 2
Claims (10)
1. a kind of preparation method of dendrobium candidum fungi fermentation product, it is characterised in that including stem of noble dendrobium preparation, actication of culture, liquid
Seed culture, stem of noble dendrobium fungi fermentation, fermented product processing step, are comprised the following steps that：
A, prepared by the stem of noble dendrobium：The fresh bar of dendrobium candidum is removed into inedible position and cleaned, segment is cut after naturally dry, mashing obtains
Stem of noble dendrobium slurry is standby；
B, actication of culture：The integration of drinking and medicinal herbs fungi strain of preservation is transferred on inclined-plane solid medium and cultivated at 28~30 DEG C
5~10d；
C, liquid seeds culture：Above-mentioned activated integration of drinking and medicinal herbs fungi strain is cut into one piece to be inoculated in equipped with 100mL liquid
In the 300mL triangular flasks of culture medium, with 140~150r/min, 7~10d of concussion and cultivate under the conditions of 28~30 DEG C, until being formed
Macroscopic mycelium and/or bacterium ball, which are covered with triangular flask, to be terminated to ferment；
D, stem of noble dendrobium fungi fermentation：By the stem of noble dendrobium being prepared slurry with fermented nutritive liquid by 2:8~3:7 material liquid volume is put than mixing
Enter 100mL in 300mL triangular flasks and sterilize, after sterilizing by the cultured fungi liquid seeds of step C by mixed liquor 10~
20% volume mass ratio is inoculated into mixed liquor, and ferment at constant temperature is covered with until forming macroscopic mycelium and/or bacterium ball
Triangular flask terminates to ferment；
E, fermented product is handled：The stem of noble dendrobium fungal fermented filtrate of D step fermentation ends is centrifuged through 1000r/min and removes thalline and stone
Dry measure used in former times slag, zymotic fluid is taken to obtain dendrobium candidum fungi fermentation product through pasteurization.
2. the preparation method of dendrobium candidum fungi fermentation product according to claim 1, it is characterised in that the integration of drinking and medicinal herbs
Fungi is grifola frondosus, dome ganoderma lucidum, Poria cocos, rainbow conk, red ganoderma lucidum, hedgehog hydnum.
3. the preparation method of dendrobium candidum fungi fermentation product according to claim 2, it is characterised in that the grifola frondosus is
G2M510 bacterial strains, G2M563 bacterial strains, G2M5164 bacterial strains, the dome ganoderma lucidum are G2M56 bacterial strains, the Poria cocos are G2M5219 bacterial strains,
The rainbow conk is G2M5178 bacterial strains, the red ganoderma lucidum are G2M5250 bacterial strains, the hedgehog hydnum are G2M5208 bacterial strains, above-mentioned bacterial strains are equal
From Guangdong Province's Culture Collection.
4. the preparation method of dendrobium candidum fungi fermentation product according to claim 1, it is characterised in that the step A be by
The fresh fresh bar of dendrobium candidum removes inedible part and cleaned, segment after naturally dry, then by material water 1:5~1:10 matter
Measure volume ratio mashing, obtain stem of noble dendrobium slurry it is sterilized after to be placed in -20 DEG C of refrigerations standby.
5. the preparation method of dendrobium candidum fungi fermentation product according to claim 1, it is characterised in that in the step B
Inclined-plane solid medium is PDA slant mediums, and the PDA inclined-plane cultures based component includes by mass percentage：Potato
20%th, the water of glucose 2%, agar 1.5~2%, chloramphenicol 0.01% and surplus.
6. the preparation method of dendrobium candidum fungi fermentation product according to claim 1, it is characterised in that in the step C
Fluid nutrient medium is PDA liquid medium, and the PDA liquid medium composition includes by mass percentage：Potato 20%, Portugal
The water of grape sugar 2%, chloramphenicol 0.01% and surplus.
7. the preparation method of dendrobium candidum fungi fermentation product according to claim 1, it is characterised in that in the D steps
Fermented nutritive liquid composition includes by mass percentage：0.13% peptone, 0.1% MgSO4, 0.2% KH2PO4And surplus
Water.
8. according to the preparation method of the dendrobium candidum fungi fermentation product of claim 1 or 7, it is characterised in that the D steps
The condition of middle stem of noble dendrobium ferment at constant temperature is：With 140~150r/min concussion and cultivates, 3~10d under 28~30 DEG C of constant temperatures.
9. the iron that the preparation method of dendrobium candidum fungi fermentation product described in a kind of claim 1 to 8 any one is prepared
Skin stem of noble dendrobium fungi fermentation product.
10. dendrobium candidum fungi fermentation product described in a kind of claim 9 is preparing food, health food, cosmetics and/or medicine
Application in product.
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