CN105935143B - The production method of ganoderma lucidum ferment - Google Patents
The production method of ganoderma lucidum ferment Download PDFInfo
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Abstract
A kind of production method of ganoderma lucidum ferment carries out glossy ganoderma fermentation by ganoderma lucidum extraction, fluid nutrient medium preparation, monomer fermentation, mixed fermentation, enzymatic hydrolysis.The invention has the advantages that: ganoderma lucidum ferment obtained has been released effectively the activity and nutritional ingredient of ganoderma lucidum, has improved the content of ganoderma lucidum polysaccharide and triterpenes effective active composition in ganoderma lucidum ferment, improve immunological regulation effect and anti-oxidation efficacy of ganoderma lucidum ferment, the taste and flavor for improving ganoderma lucidum ferment, improves fermenting speed, shortens fermentation time, improve fermentation efficiency, it is convenient, do not contain any additive, health environment-friendly, using effect is good, is conducive to promote.
Description
Technical field
The present invention relates to fermentation technical field more particularly to a kind of production methods of ganoderma lucidum ferment.
Background technique
Ganoderma lucidum (Ganoderma lucidum) is Basidiomycetes Polyporaceae (Polyporaceae) Ganoderma
(Ganodelma) general name of fungi red sesame G.lucidumkarst and purple sesame G.japonicrnLloyd has and strengthens the body resistance to consolidate the constitution
And other effects, top grade is known as by Shennong's Herbal.In recent years, a lot of to the chemical component and clinical research of Ganoderma fungi, to ganoderma lucidum
Reproduction cell --- the research of spore medical value is also quite paid attention to.
Reishi sporule brown, oval, one end is truncate, (8.5~11.2) μ m (5.2~6.9) μm, has double wall.Every
The brown internal layer of a spore generates many needle-shaped kicks, protrudes into the transparent outer layer of conidial cell wall dearly, outer layer sporoderm is smooth.Spirit
Ganoderma lucidum spore package two layers of walls it is very tough and tensile, general chemistry, physical method is difficult to be broken, thus limit to its chemistry at
Divide, the research of bioactive ingredients.
Ganoderma lucidum is in the maximum virgin forest in the whole nation, Changbai Mountain and the growth of Natural Reserves in Hainan optimum.But due to more
The excessive picking of the mankind, the storage of Wild ganoderma are fewer and fewer over year.Ganoderma lucidum damages liver caused by a variety of physical and chemical and biological factors
Wound has protective effect.No matter before liver damage occurs or after occurring, liver can all be protected by taking ganoderma lucidum, mitigate hepatic injury.
Ganoderma lucidum can promote metabolism of the liver to drug, poisonous substance, have exact curative effect for toxic hepatitis.Especially chronic hepatitis, spirit
Sesame can obviously eliminate the symptoms such as dizzy, out of strength, nauseous, uncomfortable liver area, and can effectively improve liver function, tend to indices
Normally.So ganoderma lucidum can be used for treating slow poisoning, all kinds of chronic hepatitis, cirrhosis, hepatosis.Ganoderma lucidum can be effectively
Coronary artery dilator increases coronary blood flow, improves Myocardial Microcirculation, enhances the supply of myocardium oxygen and energy, therefore, to cardiac muscle
Ischemic has protective effect, can be widely used for the treatment and prevention of coronary heart diseases and angina pectoris etc..In addition, may replace after taking ganoderma lucidum
Insulin inhibits releasing for fatty acid, can improve the symptoms such as blood glucose, glucose in urine.Water-soluble polysaccharide in ganoderma lucidum can reduce non-pancreas islet
The occurring degree of plain dependent diabetes.
The chemical component of Ganoderma is complex, and because of strain used, the strain place of production, cultural method, extraction process, preparation
Method is different and different.Contain polysaccharide, ucleosides, furan derivative, steroid in the fructification of Ganoderma, mycelium and spore
Ferment class, alkaloids, protein, polypeptide, amino acids, triterpenes, sequiterpene, organic germanium, inorganic salts etc..Ganoderma lucidum polysaccharide, three
Terpene is one of principle active component of ganoderma lucidum.
Summary of the invention
The invention solves the above-mentioned problems of the prior art, provide a kind of production method of ganoderma lucidum ferment, Neng Gouti
The content of effective component, meets the needs of glossy ganoderma fermentation in high ganoderma lucidum ferment.
The technical scheme adopted by the invention to solve the technical problem: the production method of this ganoderma lucidum ferment, including walks as follows
It is rapid:
The first step, ganoderma lucidum extraction, ganoderma lucidum is crushed, and obtains glossy ganoderma powder after crossing 50 mesh~100 mesh, is then added in glossy ganoderma powder
Enter drinking water and carry out boiling, is kept after boiling boil condition 10 minutes~30 minutes, stand 12 hours~15 hours after cooling,
Supernatant is taken after the completion of standing, then 95% ethyl alcohol is added in supernatant, after mixing evenly, 12 hours~15 hours is stood, obtains
To ganoderma lucidum ethanol solution, heating concentration then is carried out to ganoderma lucidum ethanol solution, until the volume of the concentrated liquid is ganoderma lucidum ethanol solution body
Long-pending 7%~9% obtains lucidum extracting liquid；
Second step, fluid nutrient medium preparation, chooses codonopsis pilosula powder, Radix Liriopes powder, bletilla striata powder, rhizoma atractylodis powder, wall-broken ganoderma spore
Powder, soy meal, yeast powder, glucose, vitamin B1, honey, water are fabricated to fluid nutrient medium, and wherein the dosage of codonopsis pilosula powder is 2
Part~4 parts, the dosage of Radix Liriopes powder is 1 part~3 parts, and the dosage of bletilla striata powder is 1 part~2 parts, and the dosage of rhizoma atractylodis powder is 2 parts~4
Part, the dosage of ganoderma spove powder is 6 parts~9 parts, and the dosage of soy meal is 5 parts~8 parts, the dosage of yeast powder is 8 parts~
10 parts, the dosage of glucose is 20 parts~40 parts, and the dosage of vitamin B1 is 3 parts~4 parts, and the dosage of honey is 2 parts~4 parts,
The dosage of water is 300 parts~400 parts；
Third step, monomer fermentation, the lucidum extracting liquid of acquisition is added in fluid nutrient medium, then adds lactic acid again
Bacterium is then placed in fermentor and ferments, and fermentation temperature is 26 DEG C~29 DEG C, and tank pressure is 0.04Mpa~0.05Mpa, nothing
Bacterium air vent amount is 2 cubic metres per minute, to fermentation liquid retrogradation, pH value be down to 3.0~3.5, residual sugar amount be down to 0.2%~
After 0.3%, obtain ganoderma lucidum fermented liquid, lucidum extracting liquid fermentation while, set up another fermentor, in fermentor be added pineapple,
Sugarcane, dragon fruit, lemon, pumpkin, celery, jujube, Kiwi berry, apple, pawpaw, pineapple are peeling pineapple, and sugarcane is that peeling is sweet
Sugarcane, dragon fruit are peeling dragon fruit, and lemon is peeling lemon, and pumpkin is peeling pumpkin, and Kiwi berry is peeling Kiwi berry, and apple is
Apple is removed the peel, pawpaw is peeling pawpaw, then adds lactic acid bacteria in fermentor and ferments, fermentation temperature is 26 DEG C~29
DEG C, tank pressure is 0.04Mpa~0.05Mpa, and filtrated air ventilatory capacity is 2 cubic metres per minute, is down to fermentation liquid retrogradation, pH value
3.0~3.5, after residual sugar amount is down to 0.2%~0.3%, fruits and vegetables fermentation liquid is obtained；
4th step, mixed fermentation mix the ganoderma lucidum fermented liquid of acquisition and fruits and vegetables fermentation liquid, obtain mixture, then will mix
It closes object and is placed to fermentor, then add lactic acid bacteria in the fermenter, carry out mixed fermentation, fermentation temperature when fermentation is 26 DEG C
~29 DEG C, tank pressure is that 0.04Mpa~0.05Mpa obtains ganoderma lucidum mixed fermentation liquid after fermentation 2 years；
5th step, enzymatic hydrolysis are added amylase, protease, cellulase in ganoderma lucidum mixed fermentation liquid and are digested, digested
Temperature is 50 DEG C~60 DEG C, and enzymolysis time is 5 hours~6 hours, and wherein amylase, protease, cellulase are mixed with ganoderma lucidum
The mass ratio of fermentation liquid is 1:1:1:100, obtains ganoderma lucidum ferment after enzymatic hydrolysis.
Further, the ganoderma lucidum in the first step is any one of black sesame, green sesame, purple sesame, red sesame, Bai Zhi, Huang Zhizhong.
Further, the lactic acid bacteria in third step is streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, cheese cream bar
Bacterium, lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus paracasei, Pediococcus pentosaceus, Lactobacillus brevis, Lactobacillus helveticus, goldbeater's skin are bright
Any one in beading bacterium or any three kinds of combination of the above.
Further, in the 5th step obtain ganoderma lucidum ferment after, carry out ganoderma lucidum ferment extraction, by the ganoderma lucidum ferment of acquisition into
Solid-state ganoderma lucidum ferment is obtained after horizontal high voltage homogenization, extraction, concentration, vacuum freeze drying.
Further, high-pressure homogeneous processing is carried out using high pressure homogenizer when high-pressure homogeneous processing.
Further, it is extracted when extraction using supercritical carbon dioxide method for extracting.
The production method of ganoderma lucidum ferment of the invention carries out the extraction of ganoderma lucidum in the first step, extracts lucidum extracting liquid, the
The fluid nutrient medium preparation that two steps are fermented, is added codonopsis pilosula powder, Radix Liriopes powder, bletilla striata powder, rhizoma atractylodis powder in liquid medium,
Codonopsis pilosula powder is made of Radix Codonopsis grinding, and Radix Liriopes powder is made of Radix Liriopes grinding, and bletilla striata powder is made of bletilla striata grinding, and rhizoma atractylodis powder is by grey
Art grinding is made, and Radix Codonopsis has effects that tonifying middle-Jiao and Qi, strengthening spleen and tonifying lung, and Radix Liriopes have effects that nourishing Yin and promoting production of body fluid, moistening lung and clearing away the heart-fire,
The bletilla striata has effects that astringing to arrest bleeding, detumescence and promoting granulation, and rhizoma atractylodis have effects that drying damp and strengthening spleen, expelling wind and clearing away cold, by this several taste
The effect of herbal medicine, can promote glossy ganoderma fermentation, be released effectively the activity and nutritional ingredient of ganoderma lucidum, improve ganoderma lucidum in ganoderma lucidum ferment
The content of polysaccharide, triterpene effective constituents improves immunological regulation effect and anti-oxidation efficacy of ganoderma lucidum ferment, reduces ganoderma lucidum damage
Consumption sufficiently improves ganoderma lucidum utilization rate, and ganoderma spove powder uses ganoderma spove powder identical with fermentation glossy ganoderma kind, into
One step promotes glossy ganoderma fermentation, and third step carries out monomer fermentation, and lactobacillus-fermented is added in when fermentation, can be improved the mouth of ganoderma lucidum ferment
Sense and flavor, the lactic acid bacteria of addition can be the lactic acid bacteria of a kind, be also possible to the compound lactobacillus of multiple breed combinations,
It can be realized different taste and flavors, the 4th step carries out mixed fermentation, the ganoderma lucidum fermented liquid and fruits and vegetables that monomer fermentation is obtained
Fermentation liquid carries out mixing post-fermentation, and the nutritional ingredient that fermentation material obtained can be made to have fruits and vegetables after fruits and vegetables fermentation liquid, battalion is added
It is high to support ingredient value, improves the nutritive value of fermentation material, and further increase the taste and flavor of fermentation material, the 5th stepping
Row enzymatic hydrolysis, further increases the content of effective component in ganoderma lucidum ferment, and further increase the mouthfeel of ganoderma lucidum ferment, after enzymatic hydrolysis
Last finished product ganoderma lucidum ferment is obtained, for finished product ganoderma lucidum ferment, solid ganoderma lucidum ferment can be also extracted into, by the spirit of acquisition
Sesame ferment obtains solid-state ganoderma lucidum ferment after carrying out high-pressure homogeneous processing, extraction, concentration, vacuum freeze drying, can further subtract
The loss of few ganoderma lucidum, effectively extracts solid-state ganoderma lucidum ferment.
The invention has the advantages that: the production method of ganoderma lucidum ferment of the invention, ganoderma lucidum ferment obtained are released effectively
The activity and nutritional ingredient of ganoderma lucidum, improves the content of ganoderma lucidum polysaccharide and triterpenes effective active composition in ganoderma lucidum ferment, mentions
High immunological regulation effect and anti-oxidation efficacy of ganoderma lucidum ferment, improves the taste and flavor of ganoderma lucidum ferment, improves fermentation
Speed shortens fermentation time, improves fermentation efficiency, convenient, does not contain any additive, and health environment-friendly uses effect
Fruit is good, is conducive to promote.
Specific embodiment
The invention will be further described below:
Embodiment one:
The production method of this ganoderma lucidum ferment, includes the following steps:
The first step, ganoderma lucidum extraction, ganoderma lucidum is crushed, and is obtained glossy ganoderma powder after crossing 50 mesh, is then added and drinks in glossy ganoderma powder
Water carries out boiling, keeps boil condition 10 minutes after boiling, stands 12 hours after cooling, takes supernatant after the completion of standing, then
95% ethyl alcohol is added in supernatant, after mixing evenly, stands 12 hours, ganoderma lucidum ethanol solution is obtained, then to ganoderma lucidum ethyl alcohol
Solution carries out heating concentration, until the volume of the concentrated liquid is the 7% of ganoderma lucidum volumes of aqueous ethanol, obtains lucidum extracting liquid；
Second step, fluid nutrient medium preparation, chooses codonopsis pilosula powder, Radix Liriopes powder, bletilla striata powder, rhizoma atractylodis powder, wall-broken ganoderma spore
Powder, soy meal, yeast powder, glucose, vitamin B1, honey, water are fabricated to fluid nutrient medium, and wherein the dosage of codonopsis pilosula powder is 2
Part, the dosage of Radix Liriopes powder is 1 part, and the dosage of bletilla striata powder is 1 part, and the dosage of rhizoma atractylodis powder is 2 parts, the use of ganoderma spove powder
Amount is 6 parts, and the dosage of soy meal is 5 parts, and the dosage of yeast powder is 8 parts, and the dosage of glucose is 20 parts, the dosage of vitamin B1
It is 3 parts, the dosage of honey is 2 parts, and the dosage of water is 300 parts；
Third step, monomer fermentation, the lucidum extracting liquid of acquisition is added in fluid nutrient medium, then adds lactic acid again
Bacterium is then placed in fermentor and ferments, and fermentation temperature is 26 DEG C, and tank pressure is 0.04Mpa, and filtrated air ventilatory capacity is every
It minutes 2 cubic metres, is down to after 3.0, residual sugar amount is down to 0.2% after fermentation liquid retrogradation, pH value, ganoderma lucidum fermented liquid is obtained, in ganoderma lucidum
While extracting solution ferments, fermentor is set up another, pineapple, sugarcane, dragon fruit, lemon, pumpkin, celery, red is added in fermentor
Jujube, Kiwi berry, apple, pawpaw, pineapple are peeling pineapple, and sugarcane is peeling sugarcane, and dragon fruit is peeling dragon fruit, and lemon is to go
Skin lemon, pumpkin are peeling pumpkin, and Kiwi berry is peeling Kiwi berry, and apple is peeling apple, and pawpaw is peeling pawpaw, are then existed
Addition lactic acid bacteria is fermented in fermentor, and fermentation temperature is 26 DEG C, and tank pressure is 0.04Mpa, and filtrated air ventilatory capacity is every point
It 2 cubic metres of clock, is down to after 3.0, residual sugar amount is down to 0.2% after fermentation liquid retrogradation, pH value, obtains fruits and vegetables fermentation liquid；
4th step, mixed fermentation mix the ganoderma lucidum fermented liquid of acquisition and fruits and vegetables fermentation liquid, obtain mixture, then will mix
It closes object and is placed to fermentor, then add lactic acid bacteria in the fermenter, carry out mixed fermentation, fermentation temperature when fermentation is 26
DEG C, tank pressure is that 0.04Mpa obtains ganoderma lucidum mixed fermentation liquid after fermentation 2 years；
5th step, enzymatic hydrolysis are added amylase, protease, cellulase in ganoderma lucidum mixed fermentation liquid and are digested, digested
Temperature is 50 DEG C, and enzymolysis time is 5 hours, wherein the mass ratio of amylase, protease, cellulase and ganoderma lucidum mixed fermentation liquid
For 1:1:1:100, ganoderma lucidum ferment is obtained after enzymatic hydrolysis.
Further, the ganoderma lucidum in the first step is black sesame.
Further, the lactic acid bacteria in third step is Lactobacillus delbrueckii.
Further, in the 5th step obtain ganoderma lucidum ferment after, carry out ganoderma lucidum ferment extraction, by the ganoderma lucidum ferment of acquisition into
Solid-state ganoderma lucidum ferment is obtained after horizontal high voltage homogenization, extraction, concentration, vacuum freeze drying.
Further, high-pressure homogeneous processing is carried out using high pressure homogenizer when high-pressure homogeneous processing.
Further, it is extracted when extraction using supercritical carbon dioxide method for extracting.
Embodiment two:
The production method of this ganoderma lucidum ferment, includes the following steps:
The first step, ganoderma lucidum extraction, ganoderma lucidum is crushed, and is obtained glossy ganoderma powder after crossing 80 mesh, is then added and drinks in glossy ganoderma powder
Water carries out boiling, keeps boil condition 20 minutes after boiling, stands 13 hours after cooling, takes supernatant after the completion of standing, then
95% ethyl alcohol is added in supernatant, after mixing evenly, stands 13 hours, ganoderma lucidum ethanol solution is obtained, then to ganoderma lucidum ethyl alcohol
Solution carries out heating concentration, until the volume of the concentrated liquid is the 8% of ganoderma lucidum volumes of aqueous ethanol, obtains lucidum extracting liquid；
Second step, fluid nutrient medium preparation, chooses codonopsis pilosula powder, Radix Liriopes powder, bletilla striata powder, rhizoma atractylodis powder, wall-broken ganoderma spore
Powder, soy meal, yeast powder, glucose, vitamin B1, honey, water are fabricated to fluid nutrient medium, and wherein the dosage of codonopsis pilosula powder is 3
Part, the dosage of Radix Liriopes powder is 2 parts, and the dosage of bletilla striata powder is 2 parts, and the dosage of rhizoma atractylodis powder is 3 parts, the use of ganoderma spove powder
Amount is 7 parts, and the dosage of soy meal is 7 parts, and the dosage of yeast powder is 9 parts, and the dosage of glucose is 30 parts, the dosage of vitamin B1
It is 4 parts, the dosage of honey is 3 parts, and the dosage of water is 350 parts；
Third step, monomer fermentation, the lucidum extracting liquid of acquisition is added in fluid nutrient medium, then adds lactic acid again
Bacterium is then placed in fermentor and ferments, and fermentation temperature is 27 DEG C, and tank pressure is 0.05Mpa, and filtrated air ventilatory capacity is every
It minutes 2 cubic metres, is down to after 3.2, residual sugar amount is down to 0.3% after fermentation liquid retrogradation, pH value, ganoderma lucidum fermented liquid is obtained, in ganoderma lucidum
While extracting solution ferments, fermentor is set up another, pineapple, sugarcane, dragon fruit, lemon, pumpkin, celery, red is added in fermentor
Jujube, Kiwi berry, apple, pawpaw, pineapple are peeling pineapple, and sugarcane is peeling sugarcane, and dragon fruit is peeling dragon fruit, and lemon is to go
Skin lemon, pumpkin are peeling pumpkin, and Kiwi berry is peeling Kiwi berry, and apple is peeling apple, and pawpaw is peeling pawpaw, are then existed
Addition lactic acid bacteria is fermented in fermentor, and fermentation temperature is 27 DEG C, and tank pressure is 0.05Mpa, and filtrated air ventilatory capacity is every point
It 2 cubic metres of clock, is down to after 3.2, residual sugar amount is down to 0.3% after fermentation liquid retrogradation, pH value, obtains fruits and vegetables fermentation liquid；
4th step, mixed fermentation mix the ganoderma lucidum fermented liquid of acquisition and fruits and vegetables fermentation liquid, obtain mixture, then will mix
It closes object and is placed to fermentor, then add lactic acid bacteria in the fermenter, carry out mixed fermentation, fermentation temperature when fermentation is 27
DEG C, tank pressure is that 0.05Mpa obtains ganoderma lucidum mixed fermentation liquid after fermentation 2 years；
5th step, enzymatic hydrolysis are added amylase, protease, cellulase in ganoderma lucidum mixed fermentation liquid and are digested, digested
Temperature is 55 DEG C, and enzymolysis time is 6 hours, wherein the mass ratio of amylase, protease, cellulase and ganoderma lucidum mixed fermentation liquid
For 1:1:1:100, ganoderma lucidum ferment is obtained after enzymatic hydrolysis.
Further, the ganoderma lucidum in the first step is purple sesame.
Further, the lactic acid bacteria Leuconostoc mesenteroides in third step.
Further, in the 5th step obtain ganoderma lucidum ferment after, carry out ganoderma lucidum ferment extraction, by the ganoderma lucidum ferment of acquisition into
Solid-state ganoderma lucidum ferment is obtained after horizontal high voltage homogenization, extraction, concentration, vacuum freeze drying.
Further, high-pressure homogeneous processing is carried out using high pressure homogenizer when high-pressure homogeneous processing.
Further, it is extracted when extraction using supercritical carbon dioxide method for extracting.
Embodiment three:
The production method of this ganoderma lucidum ferment, includes the following steps:
The first step, ganoderma lucidum extraction, ganoderma lucidum is crushed, and is obtained glossy ganoderma powder after crossing 100 mesh, is then added and drinks in glossy ganoderma powder
Water carries out boiling, keeps boil condition 30 minutes after boiling, stands 15 hours after cooling, takes supernatant after the completion of standing, then
95% ethyl alcohol is added in supernatant, after mixing evenly, stands 15 hours, ganoderma lucidum ethanol solution is obtained, then to ganoderma lucidum ethyl alcohol
Solution carries out heating concentration, until the volume of the concentrated liquid is the 9% of ganoderma lucidum volumes of aqueous ethanol, obtains lucidum extracting liquid；
Second step, fluid nutrient medium preparation, chooses codonopsis pilosula powder, Radix Liriopes powder, bletilla striata powder, rhizoma atractylodis powder, wall-broken ganoderma spore
Powder, soy meal, yeast powder, glucose, vitamin B1, honey, water are fabricated to fluid nutrient medium, and wherein the dosage of codonopsis pilosula powder is 4
Part, the dosage of Radix Liriopes powder is 3 parts, and the dosage of bletilla striata powder is 2 parts, and the dosage of rhizoma atractylodis powder is 4 parts, the use of ganoderma spove powder
Amount is 9 parts, and the dosage of soy meal is 8 parts, and the dosage of yeast powder is 10 parts, and the dosage of glucose is 40 parts, the use of vitamin B1
Amount is 4 parts, and the dosage of honey is 4 parts, and the dosage of water is 400 parts；
Third step, monomer fermentation, the lucidum extracting liquid of acquisition is added in fluid nutrient medium, then adds lactic acid again
Bacterium is then placed in fermentor and ferments, and fermentation temperature is 29 DEG C, and tank pressure is 0.05Mpa, and filtrated air ventilatory capacity is every
It minutes 2 cubic metres, is down to after 3.5, residual sugar amount is down to 0.3% after fermentation liquid retrogradation, pH value, ganoderma lucidum fermented liquid is obtained, in ganoderma lucidum
While extracting solution ferments, fermentor is set up another, pineapple, sugarcane, dragon fruit, lemon, pumpkin, celery, red is added in fermentor
Jujube, Kiwi berry, apple, pawpaw, pineapple are peeling pineapple, and sugarcane is peeling sugarcane, and dragon fruit is peeling dragon fruit, and lemon is to go
Skin lemon, pumpkin are peeling pumpkin, and Kiwi berry is peeling Kiwi berry, and apple is peeling apple, and pawpaw is peeling pawpaw, are then existed
Addition lactic acid bacteria is fermented in fermentor, and fermentation temperature is 29 DEG C, and tank pressure is 0.05Mpa, and filtrated air ventilatory capacity is every point
It 2 cubic metres of clock, is down to after 3.5, residual sugar amount is down to 0.3% after fermentation liquid retrogradation, pH value, obtains fruits and vegetables fermentation liquid；
4th step, mixed fermentation mix the ganoderma lucidum fermented liquid of acquisition and fruits and vegetables fermentation liquid, obtain mixture, then will mix
It closes object and is placed to fermentor, then add lactic acid bacteria in the fermenter, carry out mixed fermentation, fermentation temperature when fermentation is 29
DEG C, tank pressure is that 0.05Mpa obtains ganoderma lucidum mixed fermentation liquid after fermentation 2 years；
5th step, enzymatic hydrolysis are added amylase, protease, cellulase in ganoderma lucidum mixed fermentation liquid and are digested, digested
Temperature is 60 DEG C, and enzymolysis time is 6 hours, wherein the mass ratio of amylase, protease, cellulase and ganoderma lucidum mixed fermentation liquid
For 1:1:1:100, ganoderma lucidum ferment is obtained after enzymatic hydrolysis.
Further, the ganoderma lucidum in the first step is red sesame.
Further, the lactic acid bacteria in third step is the combination of lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum.
Further, in the 5th step obtain ganoderma lucidum ferment after, carry out ganoderma lucidum ferment extraction, by the ganoderma lucidum ferment of acquisition into
Solid-state ganoderma lucidum ferment is obtained after horizontal high voltage homogenization, extraction, concentration, vacuum freeze drying.
Further, high-pressure homogeneous processing is carried out using high pressure homogenizer when high-pressure homogeneous processing.
Further, it is extracted when extraction using supercritical carbon dioxide method for extracting.
The embodiment of the present invention one, embodiment two, in embodiment three, 1 part is 1 gram.
Choose ganoderma lucidum ferment and the progress of Common ganoderma lucidum ferment prepared by the embodiment of the present invention one, embodiment two, embodiment three
Comparison, comparing result are as follows:
As can be known from the above table, ganoderma lucidum ferment prepared by the embodiment of the present invention one, embodiment two, embodiment three, in ganoderma lucidum effectively
Component content is above Common ganoderma lucidum ferment, and mouthfeel is superior to Common ganoderma lucidum ferment.
The characteristics of embodiment of the present invention is: ganoderma lucidum ferment obtained has been released effectively the activity and nutritional ingredient of ganoderma lucidum, has mentioned
The content of ganoderma lucidum polysaccharide and triterpenes effective active composition, improves immunological regulation effect of ganoderma lucidum ferment in high ganoderma lucidum ferment
And anti-oxidation efficacy, the taste and flavor of ganoderma lucidum ferment is improved, fermenting speed is improved, shortens fermentation time, is improved
Fermentation efficiency, it is convenient, any additive is not contained, health environment-friendly, using effect is good, is conducive to promote.
Although the present invention is described by reference to preferred embodiment, those skilled in the art answer
Work as understanding, within the scope of the claims, various variation in form and details can be made.
Claims (6)
1. a kind of production method of ganoderma lucidum ferment, it is characterised in that include the following steps:
(1) ganoderma lucidum extracts, and ganoderma lucidum is crushed, and obtains glossy ganoderma powder after crossing 50 mesh~100 mesh, drinking water is then added in glossy ganoderma powder
Boiling is carried out, is kept after boiling boil condition 10 minutes~30 minutes, stands 12 hours~15 hours after cooling, stood and complete
After take supernatant, then 95% ethyl alcohol is added in supernatant, after mixing evenly, stands 12 hours~15 hours, obtain ganoderma lucidum second
Then alcoholic solution carries out heating concentration to ganoderma lucidum ethanol solution, until the volume of the concentrated liquid be ganoderma lucidum volumes of aqueous ethanol 7%~
9%, obtain lucidum extracting liquid；
(2) prepared by fluid nutrient medium, chooses codonopsis pilosula powder, Radix Liriopes powder, bletilla striata powder, rhizoma atractylodis powder, ganoderma spove powder, soybean
Powder, yeast powder, glucose, vitamin B1, honey, water are fabricated to fluid nutrient medium, and wherein the dosage of codonopsis pilosula powder is 2 parts~4 parts,
The dosage of Radix Liriopes powder is 1 part~3 parts, and the dosage of bletilla striata powder is 1 part~2 parts, and the dosage of rhizoma atractylodis powder is 2 parts~4 parts, broken wall spirit
The dosage of Ganoderma lucidum spore powder is 6 parts~9 parts, and the dosage of soy meal is 5 parts~8 parts, and the dosage of yeast powder is 8 parts~10 parts, grape
The dosage of sugar is 20 parts~40 parts, and the dosage of vitamin B1 is 3 parts~4 parts, and the dosage of honey is 2 parts~4 parts, and the dosage of water is
300 parts~400 parts；
(3) monomer ferments, and the lucidum extracting liquid of acquisition is added in fluid nutrient medium, lactic acid bacteria is then added again, then sets
Enter and ferment into fermentor, fermentation temperature is 26 DEG C~29 DEG C, and tank pressure is 0.04Mpa~0.05Mpa, filtrated air ventilation
Amount is 2 cubic metres per minute, is down to after 3.0~3.5, residual sugar amount is down to 0.2%~0.3%, obtains after fermentation liquid retrogradation, pH value
Ganoderma lucidum fermented liquid, lucidum extracting liquid fermentation while, set up another fermentor, in fermentor be added pineapple, sugarcane, dragon fruit,
Lemon, pumpkin, celery, jujube, Kiwi berry, apple, pawpaw, pineapple are peeling pineapple, and sugarcane is peeling sugarcane, and dragon fruit is to go
Skin dragon fruit, lemon are peeling lemon, and pumpkin is peeling pumpkin, and Kiwi berry is peeling Kiwi berry, and apple is peeling apple, pawpaw
To remove the peel pawpaw, lactic acid bacteria then being added in fermentor and is fermented, fermentation temperature is 26 DEG C~29 DEG C, and tank pressure is
0.04Mpa~0.05Mpa, filtrated air ventilatory capacity are 2 cubic metres per minute, be down to 3.0~3.5 to fermentation liquid retrogradation, pH value,
After residual sugar amount is down to 0.2%~0.3%, fruits and vegetables fermentation liquid is obtained；
(4) mixed fermentation mixes the ganoderma lucidum fermented liquid of acquisition and fruits and vegetables fermentation liquid, obtains mixture, then mixture is placed in
To fermentor, lactic acid bacteria is then added in the fermenter, carries out mixed fermentation, fermentation temperature when fermentation is 26 DEG C~29 DEG C,
Tank pressure is that 0.04Mpa~0.05Mpa obtains ganoderma lucidum mixed fermentation liquid after fermentation 2 years；
(5) it digests, amylase, protease, cellulase is added in ganoderma lucidum mixed fermentation liquid and is digested, hydrolysis temperature 50
DEG C~60 DEG C, enzymolysis time is 5 hours~6 hours, wherein amylase, protease, cellulase and ganoderma lucidum mixed fermentation liquid
Mass ratio is 1:1:1:100, obtains ganoderma lucidum ferment after enzymatic hydrolysis.
2. the production method of ganoderma lucidum ferment according to claim 1, it is characterised in that: the ganoderma lucidum in the step (1) is
Any one of black sesame, green sesame, purple sesame, red sesame, Bai Zhi, Huang Zhizhong.
3. the production method of ganoderma lucidum ferment according to claim 1, it is characterised in that: the lactic acid bacteria in the step (3)
For streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum, Lactobacillus rhamnosus, secondary cheese
Lactobacillus, Pediococcus pentosaceus, Lactobacillus brevis, Lactobacillus helveticus, any one or any three kinds or more in Leuconostoc mesenteroides
Combination.
4. the production method of ganoderma lucidum ferment according to claim 1, it is characterised in that: obtain ganoderma lucidum in the step (5)
After ferment, the extraction of ganoderma lucidum ferment is carried out, the ganoderma lucidum ferment of acquisition is subjected to high-pressure homogeneous processing, extraction, concentration, vacuum refrigeration
Solid-state ganoderma lucidum ferment is obtained after drying.
5. the production method of ganoderma lucidum ferment according to claim 4, it is characterised in that: step (5) the mesohigh homogeneous
High-pressure homogeneous processing is carried out using high pressure homogenizer when processing.
6. the production method of ganoderma lucidum ferment according to claim 4, it is characterised in that: adopted when extraction in the step (5)
It is extracted with supercritical carbon dioxide method for extracting.
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| CN107080130A (en) * | 2017-04-19 | 2017-08-22 | 句容金坤农业科技有限公司 | A kind of preparation method of lucidum spore powder ferment health drink |
| CN109875043A (en) * | 2019-04-28 | 2019-06-14 | 灵芝治未病(广东)科技有限公司 | A kind of ganoderma lucidum polypeptide powder and preparation method thereof rich in biodiasmin |
| CN110859305B (en) * | 2019-12-17 | 2022-10-25 | 山东省食品发酵工业研究设计院 | Method for preparing asparagus SOD enzyme by using asparagus juicing residues |
| CN110973614A (en) * | 2019-12-30 | 2020-04-10 | 武夷山元生泰生物科技有限公司 | Method for preparing ganoderma lucidum enzyme through continuous fermentation |
| CN114632053B (en) * | 2022-04-28 | 2024-02-09 | 浙江长生鸟健康科技股份有限公司 | Pearl powder enhanced antioxidant crude ganoderma sinensis extract and fermentation method thereof |
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