CN107080130A - A kind of preparation method of lucidum spore powder ferment health drink - Google Patents

A kind of preparation method of lucidum spore powder ferment health drink Download PDF

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Publication number
CN107080130A
CN107080130A CN201710257666.9A CN201710257666A CN107080130A CN 107080130 A CN107080130 A CN 107080130A CN 201710257666 A CN201710257666 A CN 201710257666A CN 107080130 A CN107080130 A CN 107080130A
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CN
China
Prior art keywords
liquid
spore powder
lucidum spore
health drink
finished
Prior art date
Application number
CN201710257666.9A
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Chinese (zh)
Inventor
赵晓联
廖祥儒
毛飞君
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句容金坤农业科技有限公司
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Priority to CN201710257666.9A priority Critical patent/CN107080130A/en
Publication of CN107080130A publication Critical patent/CN107080130A/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2220/00Lactobacillus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2220/00Lactobacillus
    • A23Y2220/67Plantarum

Abstract

The invention discloses a kind of preparation method of lucidum spore powder ferment health drink:Lucidum spore powder adds fermentation tank with water, adds honey, strain fermentation;Fermentation ends, zymotic fluid collects the concentrate that cleaner liquid and filter are retained through filtering respectively;Cleaner liquid is added into fruit syrup, A liquid semi-finished product are obtained;Add pure water dilution in concentrate, add fruit syrup and CMC Na and xanthans, obtain B liquid semi-finished product;By deployed A liquid semi-finished product, lucidum spore powder ferment health drink A, B liquid is made respectively through sterilization filling in B liquid semi-finished product respectively.The present invention fully discharges the active component in lucidum spore powder, due to probiotics fermention, produce unique local flavor, the active material that the probiotics benefit materials produced simultaneously discharge lucidum spore powder is more beneficial for absorption of human body, the health drink sweet and sour taste prepared with this method, it is nutritious, can for a long time it be drunk as health beverages.

Description

A kind of preparation method of lucidum spore powder ferment health drink

Technical field

The invention belongs to field of health-care beverage, and in particular to a kind of preparation method of lucidum spore powder ferment health drink.

Background technology

Ganoderma lucidum is also known as the precious (scientific name of Lin Zhongling, fine jade:Ganoderma Lucidum Karst) it is On Polyporaceae ganoderma lucidum Fructification.Have effects that invigorating qi for tranquilization, it is relieving cough and asthma, promote longevity.For dizziness egersis, palpitation, neurasthenia, void Phthisical cough is breathed heavily, improves immunity.In recent years, the chemical composition and clinical research to Ganoderma fungi are a lot of, and the reproduction to ganoderma lucidum is thin Born of the same parents --- the research of spore medical value is also quite paid attention to.

Reishi sporule brown, avette, one end is truncate, (8.5~ll.2) um × (5.2-6.9) um, has double wall.Each The brown internal layer of spore produces many needle-like kicks, the transparent outer layer of conidial cell wall is stretched into dearly, outer layer sporoderm is smooth.Ganoderma lucidum Very tough and tensile, the general chemistry of two layers of walls of spore parcel, physical method is difficult to be broken, therefore limit to its chemistry into Point, the research of bioactive ingredients.

The chemical composition of Reishi sporule can be divided into following a few classes:Protein and amino acids, glycopeptide class, vitamins, Hu Radish element, sterols, triterpenes, alkaloids, fatty acid, lactone and inorganic ions etc..Ganoderma lucidum spore gives protein content to reach 18%, the content of the wherein body essential amino acid such as isoleucine, leucine lysine, valine, methionine, phenylalanine is non- It is often abundant, from up to 10g/kg.Vitamins is mainly vitamin E (VE), in every 100g spores, VE contents more than 60mg, Also contain a small amount of vitamin C in addition.The content difference of polysaccharide and oligosaccharides, wherein disaccharides, trisaccharide, tetrose is rich in Reishi sporule It is 1.94,1.67 and 2.5mg/g.Steroid have the β alcohol (ergosta-7,22-diene-3-ol) of ergot steroid -7,22- ethene -3 and Cupreol.

By light microscope can differentiate spore broken wall whether.The determination experiment result of reducing sugar and polypeptide shows, No matter carried using acid, the extracting mode that water extraction or water are boiled, reduced sugar and content of peptides are substantially high in exosporium-broken spore extract solution In not exosporium-broken spore, and in broken wall spore, reduced sugar and polypeptide are obviously more easy to extract than not exosporium-broken spore.Lucidum spore powder is carried The effects such as high immunity of organisms, regulation cardiovascular system, enhancing respiratory system, improvement digestive system.

Examining and authorizing on ganoderma lucidum and conidia powder ferment fermentation processing into enzyme stoste and enzyme beverage this side at present The patent and paper in face are rarely reported.It is related to a kind of preparation method of ganoderma lucidum ferment in Chinese patent CN105935143A, Extracted by ganoderma lucidum, monomer fermentation, mixed fermentation, enzymolysis carry out the step such as glossy ganoderma fermentation and produce ganoderma lucidum ferment.But its step Complexity, excessive from raw material, fermentation quality is difficult to control to, while mixed fermentation 2 years, fermentation period is oversize.

The content of the invention

In order to overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of sweet mouthfeel is tasty, to greatest extent Release retains active component in lucidum spore powder, in a kind of fermented beverage with ganoderma lucidum fragrance simultaneously with unique ferment local-flavor Preparation method, solves existing lucidum spore powder active component and is difficult release, mouthfeel is dull, the problems such as effect is not notable.

To reach above-mentioned purpose, the present invention is achieved by the following technical solutions.

A kind of preparation method of lucidum spore powder ferment health drink, comprises the following steps:

(1) lucidum spore powder and water are added into fermentation tank, adds honey, strain fermentation;The strain is commodity production With the addible edible class of probiotics strain lactic acid bacteria one in food items;

(2) fermentation ends, zymotic fluid collects the concentrate that cleaner liquid and filter are retained through filtering respectively;

(3) cleaner liquid is added into fruit syrup, allotment is uniform, obtains A liquid semi-finished product;Add pure water dilution in concentrate, Fruit syrup and sodium carboxymethylcellulose and xanthans are added, allotment is uniform, obtains B liquid semi-finished product;(4) by deployed A liquid Lucidum spore powder ferment health drink A, B liquid is made respectively through sterilization filling in semi-finished product, B liquid semi-finished product respectively.

Further the weight ratio of lucidum spore powder and pure water is 3~10 in the step (1):100~200.

The lucidum spore powder further added in the step (1) is ganoderma spove powder.

Further honey is lime tree green molasses in the step (1), and dosage is defined by sugar addition to 5~10%.

Further fermentation temperature is controlled in 28~37 DEG C, 7~15d of fermentation time in the step (1).

Further strain is fermented by mixed bacterium in the step (1), is made up of Lactobacillus plantarum, lactobacillus thermophilus.

Further the mixed bacteria is spread cultivation and fermented bacterium is made by Lactobacillus plantarum, lactobacillus thermophilus activation respectively Liquid, its method is to access strain glycerol tube in MRS nutrient solutions by the amount of 1% volume ratio on superclean bench, cultivation temperature 30~37 DEG C, rotating speed is controlled in 60~80rpm, and incubated 36~48h in constant-temperature table, total clump count to strain liquid reaches 108More than cfu/ml;Then Lactobacillus plantarum by volume:Lactobacillus thermophilus is 6~8:2~4 mixing compositions;Add in fermentation tank The volume for entering mixed bacteria liquid is the 3~5% of total fermentating liquid volume.

Further zymotic fluid elder generation via hole diameter is 8~15 μm of bag filter coarse filtration in the step (2), then through filter membrane Aperture is 0.2~0.5 μm of tubular type film filter refined filtration.

Further A liquid semi-finished product are sugared by cleaner liquid and the preserved fruit for accounting for cleaner liquid weight 10~15% in the step (3) Slurry is mixed.

Further B liquid semi-finished product are that the pure of 5~10 times of concentrate weight is added in concentrate in the step (3) Water dilutes, and adds 15%~20% fruit syrup and 0.05~0.1% carboxymethyl cellulose for accounting for gross weight after dilution Sodium and the allotment of 0.03~0.06% xanthans are uniformly obtained.

Further sterilization method is normal-pressure sterilization in the step (4), and sterilising temp is 70-85 DEG C, and sterilization time is protected Hold 20-25min.

Beneficial effect:Compared with prior art, the advantage of the invention is that:

1) method refined using microorganism makes active material, polysaccharide and the soluble dietary fiber in lucidum spore powder Fully discharged Deng nutriment, the activity of benefit active is remained to greatest extent.Pass through probiotic microorganisms simultaneously Conversion, some organic acids of generation make these active materials, nutritional ingredient be easier to be absorbed by the body, strengthen its nutriture value Value;

2) probiotic lactobacillus has been accessed, dominant strain suppresses the growth of other miscellaneous bacterias, it is ensured that the steady quality of product.Produce Antibacterial material lactein suppresses varied bacteria growing, in the case where artificially not adding preservative, extends the shelf-life of product;

3) total time of fermentation is 10d or so, it is to avoid excessively polyoxygenated, destroys the active material discharged, while after fermentation Taste flavor is improved, with distinctive ferment local-flavor.

Embodiment

With reference to example, the present invention is described in further detail.

Embodiment 1

A kind of preparation method of lucidum spore powder ferment health drink, comprises the following steps:

(1) prepared by strain:Lactobacillus plantarum, two kinds of bacterial strains of lactobacillus thermophilus are cultivated respectively, are lived respectively by pure strains cultivation Change, which spreads cultivation, is made fermented bacterium liquid:Commodity production will be preserved respectively preserves strain glycerol tube on superclean bench by 1% volume In the amount access MRS nutrient solutions of ratio, 30 DEG C of cultivation temperature, rotating speed is controlled in 60rpm, the incubated 48h in constant-temperature table, extremely Total clump count of strain liquid is up to 108More than cfu/ml;

(2) raw material weighing:By lucidum spore powder and pure water by weight 3 parts:100 parts of ratio adds fermentation tank;

(3) sugar addition:Lime tree green molasses sugar addition is added to 5%;

(4) it is inoculum fermented:Mixed bacteria liquid (Lactobacillus plantarum strain liquid, thermophilic breast are added by 3% (v/v) inoculum concentration 6 parts by volume of bacillus species liquid:2 parts of compositions) fermentation, fermentation temperature is 28 DEG C, fermentation time 15d;

(5) filter:Fermentation ends, zymotic fluid is through 8 μm of aperture bag filter coarse filtration, then via hole diameter is 0.2 μm of tubular membrane Filter refined filtration, collects the concentrate of cleaner liquid and filter retention respectively;

(6) allocate:Cleaner liquid is added and accounts for 10% fruit syrup of cleaner liquid weight, allotment is uniform, obtain A liquid half into Product;The pure water dilution of 5 times of concentrate weight is added in concentrate, the fruit syrup for accounting for gross weight 15% after dilution is added With 0.05% sodium carboxymethylcellulose (CMC-Na) and 0.03% xanthans, allotment uniformly, obtains B liquid semi-finished product;

(7) sterilization filling:By deployed A liquid semi-finished product, B liquid semi-finished product are respectively through normal-pressure sterilization, sterilising temp 70 DEG C, sterilization time keeps 25min.Filling obtained lucidum spore powder ferment health drink A, the B liquid of difference.

Embodiment 2

A kind of preparation method of lucidum spore powder ferment health drink, comprises the following steps:

(1) prepared by strain:Lactobacillus plantarum, two kinds of bacterial strains of lactobacillus thermophilus are cultivated respectively, are lived respectively by pure strains cultivation Change, which spreads cultivation, is made fermented bacterium liquid:Commodity production will be preserved respectively preserves strain glycerol tube on superclean bench by 1% volume In the amount access MRS nutrient solutions of ratio, 35 DEG C of cultivation temperature, rotating speed is controlled in 70rpm, the incubated 40h in constant-temperature table, extremely Total clump count of strain liquid is up to 108More than cfu/ml;

(2) raw material weighing:Lucidum spore powder and pure water are pressed 5 parts:100 parts of ratio adds fermentation tank;

(3) sugar addition:Lime tree green molasses sugar addition is added to 8%;

(4) it is inoculum fermented:Adding mixed bacteria by 4% (v/v) inoculum concentration, (mixed bacteria is by Lactobacillus plantarum, thermophilic 7 parts by volume of lactobacillus:3 parts of compositions) fermentation, fermentation temperature is 34 DEG C, fermentation time 10d;

(5) filter:Fermentation ends, zymotic fluid is through 10 μm of aperture bag filter coarse filtration, then via hole diameter is 0.4 μm of tubular membrane Filter refined filtration, collects filtered solution and retention concentrate respectively;

(6) allocate:Clear liquid will be filtered add and account for 12% fruit syrup of clear liquid weight, allotment is uniform, obtain A liquid half into Product;The pure water dilution of 8 times of concentrate weight is added in concentrate, the fruit syrup for accounting for gross weight 18% after dilution is added With 0.08% CMC-Na and 0.05% xanthans, allotment uniformly, obtains B liquid semi-finished product;

(7) sterilization filling:By deployed A liquid semi-finished product, B liquid semi-finished product are respectively through normal-pressure sterilization, sterilising temp 80 DEG C, sterilization time keeps 22min.Filling obtained lucidum spore powder ferment health drink A, the B liquid of difference.

Embodiment 3

A kind of preparation method of lucidum spore powder ferment health drink, comprises the following steps:

(1) prepared by strain:Lactobacillus plantarum, two kinds of bacterial strains of lactobacillus thermophilus are cultivated respectively, are lived respectively by pure strains cultivation Change, which spreads cultivation, is made fermented bacterium liquid:Commodity production will be preserved respectively preserves strain glycerol tube on superclean bench by 1% volume In the amount access MRS nutrient solutions of ratio, 37 DEG C of cultivation temperature, rotating speed is controlled in 80rpm, the incubated 36h in constant-temperature table, extremely Total clump count of strain liquid reaches more than 108cfu/ml;

(2) raw material weighing:Lucidum spore powder and pure water are pressed 10 parts:200 parts of ratio adds fermentation tank;

(3) sugar addition:Lime tree green molasses sugar addition is added to 10%;

(4) it is inoculum fermented:Adding mixed bacteria by 5% (v/v) inoculum concentration, (mixed bacteria is by Lactobacillus plantarum, thermophilic 8 parts by volume of lactobacillus:4 parts of compositions) fermentation, fermentation temperature is 37 DEG C, fermentation time 7d;

(5) filter:Fermentation ends, zymotic fluid is through 15 μm of aperture bag filter coarse filtration, then via hole diameter is 0.5 μm of tubular membrane Filter refined filtration, collects filtered solution and retention concentrate respectively;

(6) allocate:Clear liquid will be filtered add and account for 15% fruit syrup of clear liquid weight, allotment is uniform, obtain A liquid half into Product;The pure water dilution of 10 times of concentrate weight is added in concentrate, the preserved fruit sugar for accounting for gross weight 20% after dilution is added The xanthans of slurry and 0.1% CMC-Na and 0.06%, allotment is uniform, obtains B liquid semi-finished product;

(7) sterilization filling:By deployed A liquid semi-finished product, B liquid semi-finished product are respectively through normal-pressure sterilization, sterilising temp 85 DEG C, sterilization time keeps 20min.Filling obtained lucidum spore powder ferment health drink A, the B liquid of difference.

In order to embody the validity of the inventive method, following table provides fermented beverage and common spirit prepared by the embodiment of the present invention The detection of sesame ferment contrast:

The present invention is illustrated according to above-described embodiment, it will be appreciated that above-described embodiment does not limit this in any form Invention, the technical scheme that all use equivalent substitution or equivalent transformation mode are obtained is within the scope of the present invention.

Claims (10)

1. a kind of preparation method of lucidum spore powder ferment health drink, it is characterised in that comprise the following steps:
(1)Lucidum spore powder and water are added into fermentation tank, honey, strain fermentation is added;The strain is eaten for commodity production The addible edible class of probiotics strain lactic acid bacteria one in items record;
(2)Fermentation ends, zymotic fluid collects the concentrate that cleaner liquid and filter are retained through filtering respectively;
(3)Cleaner liquid is added into fruit syrup, A liquid semi-finished product are obtained;Add pure water dilution in concentrate, add fruit syrup With sodium carboxymethylcellulose and xanthans, B liquid semi-finished product are obtained;
(4)By deployed A liquid semi-finished product, lucidum spore powder ferment is made respectively through sterilization filling and protects respectively for B liquid semi-finished product Strong beverage A, B liquid.
2. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 1, it is characterised in that described Step(1)The weight ratio of middle lucidum spore powder and pure water is 3 ~ 10:100~200.
3. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 1, it is characterised in that described Step(1)The lucidum spore powder of middle addition is ganoderma spove powder.
4. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 1, it is characterised in that described Step(1)Middle honey is lime tree green molasses, and dosage is defined by sugar addition to 5 ~ 10%.
5. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 1, it is characterised in that described Step(1)Middle fermentation temperature control is in 28 ~ 37 DEG C, 7 ~ 15d of fermentation time.
6. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 1, it is characterised in that described Step(1)Middle strain is fermented by mixed bacterium, is made up of Lactobacillus plantarum, lactobacillus thermophilus.
7. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 6, it is characterised in that described Mixed bacteria is spread cultivation and fermented bacterium liquid is made by Lactobacillus plantarum, lactobacillus thermophilus activation respectively, and its method is by strain glycerine Pipe on superclean bench by the amount access MRS nutrient solutions of 1% volume ratio, 30 ~ 37 DEG C of cultivation temperature, rotating speed control 60 ~ 80rpm, incubated 36 ~ 48h in constant-temperature table, to total clump count of strain liquid up to 108More than cfu/ml;Then volume is pressed Compare Lactobacillus plantarum:Lactobacillus thermophilus is 6 ~ 8:2 ~ 4 mixing compositions;It is total fermentation that the volume of mixed bacteria liquid is added in fermentation tank The 3 ~ 5% of liquid product.
8. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 1, it is characterised in that described Step(2)The first via hole diameter of middle zymotic fluid is 8 ~ 15 μm of bag filter coarse filtration, then through filter sizes is 0.2 ~ 0.5 μm of tubular membrane Filter refined filtration.
9. the preparation method of a kind of lucidum spore powder ferment health drink according to claim 1, it is characterised in that described Step(3)Middle A liquid semi-finished product are mixed by cleaner liquid with the fruit syrup for accounting for cleaner liquid weight 10 ~ 15%.
10. a kind of preparation method of lucidum spore powder ferment health drink according to claim 1, it is characterised in that institute State step(3)Middle B liquid semi-finished product add to add the pure water dilution of 5 ~ 10 times of concentrate weight in concentrate and account for dilution 15% ~ 20% fruit syrup and 0.05 ~ 0.1% sodium carboxymethylcellulose and 0.03 ~ 0.06% xanthans of gross weight afterwards Allotment is uniform and obtains.
CN201710257666.9A 2017-04-19 2017-04-19 A kind of preparation method of lucidum spore powder ferment health drink CN107080130A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105192798A (en) * 2015-09-09 2015-12-30 安徽管仲宫神生物科技有限公司 Compound enzyme beverage and preparation method thereof
CN105935143A (en) * 2016-06-29 2016-09-14 浙江蕴本生物科技有限公司 Manufacturing method of lucid ganoderma ferment
CN106174501A (en) * 2016-07-15 2016-12-07 江南大学 A kind of manufacture method of spirit Chinese holly ferment

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105192798A (en) * 2015-09-09 2015-12-30 安徽管仲宫神生物科技有限公司 Compound enzyme beverage and preparation method thereof
CN105935143A (en) * 2016-06-29 2016-09-14 浙江蕴本生物科技有限公司 Manufacturing method of lucid ganoderma ferment
CN106174501A (en) * 2016-07-15 2016-12-07 江南大学 A kind of manufacture method of spirit Chinese holly ferment

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
何革: "《生活中的中医药 2》", 31 December 2016 *

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