CN104473285A - Preparation method for traditional Chinese medicine enzyme - Google Patents
Preparation method for traditional Chinese medicine enzyme Download PDFInfo
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- CN104473285A CN104473285A CN201510011300.4A CN201510011300A CN104473285A CN 104473285 A CN104473285 A CN 104473285A CN 201510011300 A CN201510011300 A CN 201510011300A CN 104473285 A CN104473285 A CN 104473285A
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Abstract
The invention discloses a preparation method for a traditional Chinese medicine enzyme. The preparation method comprises the steps that (a) digestion is performed on a traditional Chinese medicinal material, so that an extracting solution is obtained; (b) the temperature of the traditional Chinese medicinal material extracting solution is controlled below 50 DEG C; (c) the traditional Chinese medicinal material extracting solution, an enzyme raw material and glace sauce are placed into an enzyme preparation container for fermentation, wherein the enzyme raw material comprises at least one type of living plant tissue; (d) liquid in the enzyme preparation container is separated. The invention further discloses a reasonable osmotic pressure range during fermentation of the traditional Chinese medicine enzyme. Traditional Chinese medicine enzyme prepared according to the preparation method is more reliable and effective in disease prevention and treatment. Compared with a common traditional Chinese medicine decoction, the traditional Chinese medicine enzyme is palatable, easier to store and convenient to take quickly for a long time. The preparation method is high in operability, suitable for large-scale commercial production and also suitable for home making.
Description
Technical field
The present invention relates to a kind of preparation method of health drink, especially relate to a kind of preparation method with the Chinese medicine ferment of herbal function.
Background technology
At present, known pectase preparation method is loaded by a certain percentage in container the ferment raw materials such as fresh fruit of vegetables and sugaring condiment, and the fermentation carrying out a period of time forms.Ferment raw material is generally the fresh plant tissue of stripping and slicing.Sometimes being one or more fruit, is the combination of fruits and vegetables sometimes.Sugaring condiment is the pickling material that one or more pickled sugar are formed.Pickled sugar comprises the rock sugar, fructose, brown sugar, honey, compound sugar, polysaccharide, glucose, maltose, galactolipin, trehalose etc. that can be dissolved in water.Sometimes in order to obtain better local flavor, various salt is also added in the formula of ferment sugaring condiment, as sodium chloride, calcium chloride, sodium alginate, calcium bicarbonate, potassium citrate etc.The finished product ferment made, usually in acid, is easy to preserve.Edible finished product ferment can obtain the nutritional labeling after fruits and vegetables fermentation at any time, plays certain health-care effect.Although pectase can extract the functional component in fruits and vegetables, the health-care efficacy of common fruits and vegetables is natively limited, and the usual effect of disease therapy is not strong accordingly.Therefore, the deficiency that the existing ferment preparation method medicinal efficacy that there is finished product ferment is lower.
Summary of the invention
In order to the deficiency that the medicinal efficacy overcoming existing pectase preparation method finished product ferment is lower, the invention provides a kind of preparation method of Chinese medicine ferment, the preparation method of this Chinese medicine ferment can not only make the finished product ferment of making have the rear original effect of fruits and vegetables fermentation, be easy in acidity preserve, and there is the medicinal efficacy of Chinese medicine.
The technical solution adopted for the present invention to solve the technical problems is, makes in container and loads ferment raw material and sugaring condiment ferments, it is characterized in that comprising the following steps: at ferment
A. by least one prepared slices of Chinese crude drugs heating lixiviate at least one times, Chinese medicine extract is obtained,
B. traditional Chinese medicine extraction liquid temp described in step a is controlled to less than 50 degree Celsius,
C. by Chinese medicine extract described in step b and ferment raw material and sugaring condiment loading ferment making container, ferment more than at least 4 hours, above-mentioned ferment raw material comprises the fresh and alive plant tissue of at least one, and fermentation start time zymotic fluid acetic acid concentration lower than 1%, and fermentation start time zymotic fluid concentration of alcohol lower than 10%
D., after fermentation described in completing steps c, separate ferment makes the liquid in container.
In step c, ferment makes after container loads Chinese medicine extract and ferment raw material and sugaring condiment, and when starting to ferment, the osmotic pressure of zymotic fluid controls between 0.5MPa to 5.1MPa.
In step a, the functional component extracting Chinese medicine (or Chinese traditional medicine composition) carries out according to the known process of preparing Chinese medicine and leach extraction method.For the raw herb fermenting of direct use, the poisoning risk that non-standard use Chinese medicine causes can be avoided.
Regular Chinese medicine extracting technology is all obtain medicine soup with water or the aqueous solution (wine, vinegar, pupil urine) after heating Chinese medicine medicine materical crude slice a period of time.Whole leaching process does not add sugar usually.The difference of effect material contained by Chinese medicine, heating-up temperature during lixiviate is generally at 60-90 degree Celsius.In order to raise the efficiency, sometimes also ultrasonic wave or microwave radiation technology can be supported the use.Along with the development of modern processing, wall-breaking machine is also more and more for traditional Chinese medicine extraction.Fluid separation applications after prepared slices of Chinese crude drugs lixiviate is gone out (single or polynary) chemical composition of Chinese materia medica, and then above-mentioned chemical composition of Chinese materia medica is dissolved again or is suspended in water (or fresh plant leachate) the inner technology forming Chinese medicine extract and be also applied gradually.The present invention adopts these known Chinese medicine leach extraction methods to ensure that the reliable extraction of Chinese medicine (or Chinese traditional medicine composition) property of medicine composition, control poisoning risk to a certain extent, Chinese medicine extract can be avoided simultaneously to contain pickled sugar and cause (subsequent fermentation) to be difficult to accurately feed intake by ferment making ratio or osmotic pressure requirement.
" Chinese medicine " of the present invention, if no special instructions, is often referred to " prepared slices of Chinese crude drugs " through concocting.
" fermentation " of the present invention, then refer to lactic acid bacteria or/and fungi is the fermentation mainly participating in bacterium, not marinate and wine stain.Even if because the object of some extraction, must add vinegar or wine, when fermentation starts, the acetic acid concentration (quality) of zymotic fluid also should lower than 1%, and the concentration of alcohol of zymotic fluid (quality) also should, lower than 10%, cause fermentation participate in the apoptosis of bacterium and significantly slow down fermentation to avoid acetic acid and ethanol.The tolerable temperature that above-mentioned fermentation participates in bacterium is all less than 50 degree Celsius.Step b ensure that the heat of ferment extract can not be killed these fermentations and participate in bacterium, causes fermenting unsuccessfully.
In step c, Chinese medicine extract adds round and pickled sugar forms the zymotic fluid with certain osmotic pressure, and the biological effect composition of Chinese medicine can be present in above-mentioned zymotic fluid naturally in a large number.Substitute water with this Chinese medicine extract and add round, obviously make in ferment zymotic fluid, to add the non-existent chemical composition of Chinese materia medica of common ferment.In order to promote the concentration of chemical composition of Chinese materia medica, zymotic fluid all uses medicinal plant extraction and is diluted with water no longer in addition under normal circumstances; Also can use the suspension of concentrated Chinese medicine extract or purification chemical composition of Chinese materia medica if necessary, to ensure the dose requirements of herbal function.Contained by above-mentioned zymotic fluid, chemical composition of Chinese materia medica is usually non-degradable in fermentation.(even if minority chemical composition of Chinese materia medica has the possibility be degraded, and also can evade until eliminate this degraded by selecting fermentation participation bacterium and fermentation condition).Therefore, the medicinal ingredient of Chinese medicine finally can embody in finished product ferment, thus achieves the object that finished product ferment has the medicinal efficacy of Chinese medicine.
For the Chinese medicine that some (functional component) extracting efficiencies are not high, the Chinese medicine slag after lixiviate can also be added together with extract in ferment making container and ferment.The organic solvent such as multiple organic acid, Organic Alcohol, organic ether, ethyl acetate produced in fermentation, again extracts long-time the functional component do not extracted in Chinese medicine slag, makes Chinese medicine give play to more thoroughly efficacy effect.
" fresh and alive plant tissue " of the present invention, refer to " percentage of water loss is less than 20%, and the plant roots of cell viability more than 30%, stem, fruit, blade, flower, bud ".Comprise fresh fruit, fresh vegetables and edible flower etc.During fermentation, the existence of these fresh and alive food materials provides abundance, comprehensively nutrition to the bacterium participating in fermentation, thus ensure that fermented quality.Above-mentioned add fresh plant tissue as substrate fermentation mode be used alone compared with Chinese medicine extract direct fermentation, fermentation participates in the sustainable acquisition of bacterium abundant carbon source, nitrogenous source, minerals and vitamins, and therefore breeding and metabolism all can be more stable.In addition, fresh fruits and vegetables surface usually all carries wild fungi and wild lactic acid bacteria, and these wild strains can produce positive effect to fermentation, is very beneficial for the health-care efficacy improving tunning.
The invention has the beneficial effects as follows, the pectase produced superposes the result for the treatment of of Chinese medicine on the health-care efficacy that it is original. can be more reliable to the control of disease, more effectively.This drink containing traditional Chinese medicine ingredients, is acidity and has certain osmotic pressure, is therefore easier to preserve: under the same terms, the memory time of Chinese medicine ferment is than long more than 10 times of common traditional Chinese herbal decoction.Due to containing sour-sweet vegetables and fruits fermented juice, the Chinese medicine ferment that the present invention makes is more agreeable to the taste than common Chinese decoction, thus makes people more be happy to take in a large number for a long time.This improves disease prevention and cure effect of manufactured goods from another aspect.The present invention adopts traditional process of preparing Chinese medicine and extracting technology, ensure that the basic security that Chinese medicine uses.By high-quality fermentation, the some toxic of Chinese medicine can even be eliminated in further weakening, and the easier bedding and clothing user absorption of modification by fermentation of part traditional Chinese medicine ingredients.For the traditional decoction of simple decoction, the present invention has opened up a kind of new form of Chinese drug.The present invention significantly improves health-care hospital effect of edible ferment and traditional Chinese herbal decoction, workable, is applicable to large-scale commercial applications and produces, be also applicable to Homemade simultaneously.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the fermentation schematic diagram of preparation method first embodiment of Chinese medicine ferment.
Fig. 2 is the fermentation schematic diagram of preparation method second embodiment of Chinese medicine ferment.
Fig. 3 is the pickled schematic diagram of preparation method the 3rd embodiment of Chinese medicine ferment.
Fig. 4 is the fermentation schematic diagram of preparation method the 3rd embodiment of Chinese medicine ferment.
Fig. 5 is the fermentation schematic diagram of preparation method the 4th embodiment of Chinese medicine ferment.
Fig. 6 is the fermentation schematic diagram of preparation method the 5th embodiment of Chinese medicine ferment.
In figure, 1. ferment make container, 2. ferment raw material, 3. sugaring condiment, 4. Chinese medicine extract, 5. leach sugared wine with dregs, 6. Chinese medicine slag, 7, fermentation water level.
Detailed description of the invention
All features disclosed in this description, or disclosed all methods, or the step in process, all can be combined in any way except mutually exclusive feature and/or step.
In first embodiment shown in Fig. 1, a kind of preparation method of Chinese medicine ferment, loads ferment raw material in ferment making container and sugaring condiment ferments, and it is characterized in that comprising the following steps:
A. by least one prepared slices of Chinese crude drugs heating lixiviate at least one times, Chinese medicine extract is obtained,
B. traditional Chinese medicine extraction liquid temp described in step a is controlled to less than 50 degree Celsius,
C. by Chinese medicine extract described in step b and ferment raw material and sugaring condiment loading ferment making container, ferment more than at least 4 hours, above-mentioned ferment raw material comprises the fresh and alive plant tissue of at least one, and fermentation start time zymotic fluid acetic acid concentration lower than 1%, and fermentation start time zymotic fluid concentration of alcohol lower than 10%
D., after fermentation described in completing steps c, separate ferment makes the liquid in container,
In step c, ferment makes after container loads Chinese medicine extract and ferment raw material and sugaring condiment, and when starting to ferment, osmotic pressure of fermentation liquor controls between 0.5MPa to 5.1MPa.
Pineapple contains proteinase (pineapple protease), has certain the effect ofactivating blood circulation to dissipate blood stasis.Hematoblastic deposition in blood vessel can be reduced after having data to show edible pineapple, reduce the cardiopathic death rate.But pineapple directly eats, threat irritated is on the one hand comparatively large, and overeating on the other hand to cause headache.Adopt zymotechnique, be made into pineapple ferment and can eliminate this allergic phenomena, and the part-structure of unfavorable human body in bromelain digestive ferment can be changed, make it be easier to be absorbed by the body.Even like this, effect of pineapple ferment is still lower, significantly can not change the ischemia symptom of cardiac muscle, can not promote the growth of cardiac muscle.If introduce Chinese medicine ginseng in ferment, then greatly can promote cardiac stimulant effect of finished product ferment, become a fermented beverage coronary heart disease being had to reliable action.Simultaneously as a kind of liquid, be directly used in the angina pectoris that treatment and prevention coronary heart disease and myocardial ischemia cause.
In step a, by 100 grams of ginsengs (medicine materical crude slice), with the hot water of 1500 grams 80-90 degree Celsius lixiviate 1 hour.The Chinese medicine extract 4 obtained thus is containing abundant TSPG.Multiple monomer types wherein all have the medicinal efficacy improving myocardial ischemia symptom, promote cardiac muscle growth.
The ferment of the present embodiment makes the safe fermentation tank that 2.5 liters of capacity selected by container 1.Exhaust check device with sealing fluid and exhaust leakage proof gasket can keep anaerobic environment excellent in above-mentioned fermentation tank.
FOS selected by sugaring condiment 3.Ferment raw material 2 all uses this fresh and alive plant tissue of pineapple fresh fruit.Pineapple consumption is 500-1500 gram, and the present embodiment is preferably 1000 grams.Pineapple is cleaned and dries, reject Lao Pi, cut into large slices.FOS and pineapple block layering interval are placed in safe fermentation tank.Add Ginseng extract to fermentation water level 7, submergence pineapple block and FOS.(temperature of Ginseng extract need drop to less than 50 degree Celsius, is preferably 40 degree Celsius.）
After capping, the wild strain that pineapple peel carries can start propagation.The wild lactic acid bacteria being applicable to anaerobic environment can be first developed, and becomes advantage fermented bacterium.Through the anaerobic fermentations of more than 4 hours (the present embodiment is preferably 20 days), lactic acid bacteria can produce abundant lactic acid and other metabolins (enzyme of multi-series and exocellular polysaccharide).The sensitization fragment of bromelain digestive ferment can be destroyed, and becomes not sensitization and is easy to effect active material of absorption of human body, and separates out in the Free water (solution) of zymotic fluid and pineapple.Panaxoside in zymotic fluid, also can be partially converted into the new chemical composition that effect increases, as Protopanaxatriol, protopanoxadiol in zymotic fluid.
After having fermented, in steps d, zymotic fluid is leached and namely obtains finished product Chinese medicine ferment---ginseng pineapple ferment.Proteinase in this ferment can prevent platelet deposition, softening blood vessel, lightening heart load, health-care efficacy basic after having pineapple fermentation.Meanwhile, myocardial ischemia symptom can be improved again from the TSPG of Chinese medicine ginseng and Protopanaxatriol, protopanoxadiol, promote cardiac muscle growth.This Chinese medicine ferment can more effectively cardioprotection, coronary heart diseases and angina pectoris is had to the effect of significant prevention and therapy.This preventive and therapeutic effect is more effective than simple pineapple ferment, also effective than simple ginseng decoction, even mixes also more effective than pineapple ferment and ginseng decoction simple.
In order to promote fermented quality, participate in except the growth of bacterium except the oxygen content controlling yeasting makes it be applicable to fermentation, the osmotic pressure controlling zymotic fluid is also extremely important.The osmotic pressure of zymotic fluid controls usually to adopt actual measurement empirical method and rough calculation method to realize.
Measurement method is by adding sugaring condiment respectively in a certain amount of round, measures the osmotic pressure of zymotic fluid and complete with osmotic pressure tester.More loaded down with trivial details, but can analogize later and repeatedly apply.
Rough calculation rule by the molar concentration of solute in zymotic fluid, with following osmotic pressure formula to calculating:
π=cRT
In formula, π is the osmotic pressure of solution, and c is the molar concentration of solution, and R is gas constant, and T is absolute temperature, is all defaulted as 298.15K(normal temperature 25 DEG C in the present invention).The unit of π is kPa, and when the unit of volume in molar concentration is for rising (L), R value is 8.31kPaLK
-1mol
-1
Traditional Chinese medicine ingredients is substantially all the material that molecular weight is huge, less to osmotic pressure contribution as solute.If do not add sugar material in Chinese medicine lixiviate, then that can not include solution molar concentration in counts scope.In order to simplified operation difficulty, the material that the osmotic pressure of zymotic fluid generally only chooses that molecular weight ratio 3 sugar is lower, content is greater than 0.5% calculates.The present embodiment is the solute including calculating in (containing in pineapple) glucose, fructose and (containing in sugaring condiment) FOS.
The consumption of FOS is set as 1000 grams.When Chinese medicine extract 4 is added to fermentation water level 7, the volume of zymotic fluid is appraised and decided by dischargeable capacity 2.5 liters.Substitute into above-mentioned osmotic pressure computing formula, the osmotic pressure of zymotic fluid is 3.5MPa.Between 0.5MPa to 5.1MPa.Under this osmotic pressure, harmful pathogenic bacteria can be inhibited, and fermentation simultaneously participates in bacterium (lactic acid bacteria) but can not be subject to too large suppression.Rational osmotic pressure and anaerobic environment, be enough to allow anaerobic fermentation efficiently carry out, thus the extraction completed pineapple protease and TSPG and beneficial conversion, form stronger treatment and health effect.
In the embodiment of second shown in Fig. 2, the kind of ferment making container 1, sugaring condiment 3, ferment raw material 2, Chinese medicine used is all identical with embodiment one with quantity.But, in step c, Chinese medicine slag remaining after lixiviate is added ferment making container and together ferments.
Effect material contained in ginseng is many.These effect materials can not all extract by one twice lixiviate.After lixiviate also there is considerable effect material in remaining Chinese medicine slag 6.Dropped in ferment making container 1 and fermented together, can continue to extract remaining effect material.
Chinese medicine slag 6 can all drop into, and also can choose wherein part and be placed on co-fermentation in ferment making container 1.As a preferred version, select whole ginseng after lixiviate to be placed on ferment and make container 1 co-fermentation, the part gone to sticks and staves then separately is used as him.
In the embodiment of the 3rd shown in Fig. 3, Fig. 4, ferment makes container 1, sugaring condiment 3 is identical with embodiment one.Ferment raw material 3 uses dragon fruit and each 0.5 kilogram of carrot.Chinese medicine selects the Radix Astragali and each 50 grams of Radix Angelicae Sinensis.
In step a, the prepared slices of Chinese crude drugs are immersed in water, are ground into Chinese medicine extract with high speed juice extractor.High speed juice extractor refers to the juice extractor rotating broken content with cutter group, and rotating speed is usually more than 1000 revs/min.The present embodiment selects the wall-breaking machine of cutter group turn up 37000 revs/min.The Radix Astragali and Radix Angelicae Sinensis and 0.5 premium on currency load in juice extractor bowl.Start while juice extractor carries out squeezing the juice at a high speed, cutter group frictional heat stirs liquid to Chinese medicine and heats, thus forms high temperature Chinese medicine extract 4.The Radix Astragali becomes powder with the Chinese medicine slag after Radix Angelicae Sinensis lixiviate also broken wall, is suspended in above-mentioned Chinese medicine extract 4.The extract Temperature fall of this high temperature just can add ferment to 40 degree and make in container 1.
In step c, ferment raw material mixes with sugaring condiment and produces after weight is no less than the leaching sugar wine with dregs of sugaring condiment 30%, then adds Chinese medicine extract.In the present embodiment, dragon fruit and carrot are cut sheet, and FOS layering interval be contained in ferment make in container 1 leave standstill 24 hours.Dragon fruit and carrot can be separated out Free water and be dissolved nearly all FOS, are formed and leach sugared wine with dregs 5.Now, can there is plasmolysis in dragon fruit and carrot cell, and the superoxide dismutase in dragon fruit and the beta carotene in carrot are discharged.
The osmotic pressure leaching sugared wine with dregs 5 is higher, usually all more than 5.1MPa.This hyperosmosis can suppress the quick growth of nearly all bacterium and fungi, so now fermentation can't start fast.After adding the Chinese medicine extract 4 cooling to 40 degree Celsius, the zymotic fluid be made up of Chinese medicine extract and the sugared wine with dregs of leaching, overall osmotic pressure can drop to the scope of the breeding of applicable probio and metabolism.Now weaken the suppression of bacterium and fungi that some participate in fermentation, fermentation is carried out fast.
In order to improve fermented quality and reliability, in step c, inoculated fungi or bacterium are to zymotic fluid.The fermentation of the present embodiment inoculation participates in bacterium and selects bifidobacterium adolescentis and Lactobacillus plantarum.Fermentation participates in bacterium and refers to the bacterial strain disliked gas attribute adaptation round, account for competitive advantage in fermentation.Bifidobacterium adolescentis and Lactobacillus plantarum are all anaerobic bacterias, and the ferment of the present embodiment makes the safe fermentation tank of container 1() be typically dislike air-capacitor device.In the anaerobic environment that above-mentioned suspicion air-capacitor device is formed fast, the multiple metabolins such as bifidobacterium adolescentis and Lactobacillus plantarum meeting Fast-propagation metabolism generation lactic acid.
The fermentation of inoculation participates in bacterium and can select the activated freeze-dried powder of tool, also can select the finished product ferment participating in bacterium containing fermentation.The present embodiment is preferably freeze-dried powder.After bifidobacterium adolescentis and Lactobacillus plantarum bacterium powder activate, then add in ferment making container 1.
The fermentation time of the present embodiment is preferably 20 days.Radix Astragali aglycon and Radix Angelicae Sinensis polysaccharide by fermentation, are more easily absorbed.
In steps d, the solid content after fermentation is squeezed the juice, gets the juice of squeezing out and mix with zymotic fluid.Thus, obtain the finished product ferment of high concentration.The present embodiment selects juice extractor at a slow speed.Be preferably the vertical helical propeller Normal juice machine of rotating speed 40 revs/min.A lot of Free water solution is comprised, its abundant bacterium source ferment and plant source ferment all than more in zymotic fluid in solid content after fermentation.After squeezing the juice, mixed culture fermentation liquid is taken together, and effect is stronger than taking separately zymotic fluid.
In order to preserve finished product ferment better, and improving mouthfeel, in step c or steps d, adding vinegar or wine to zymotic fluid.The present embodiment is preferably: when the solid content that ferments squeezes the juice mixed culture fermentation liquid, (in steps d) adds 6 degree of light-coloured vinegars 150 milliliters.
In the 4th embodiment shown in Fig. 5, ferment making container 1, sugaring condiment 3, ferment raw material 3, Chinese medicine are selected all identical with embodiment three.
Angelica sinensis, Radix Astragali medicine materical crude slice are pulverized without wall-breaking machine, directly with the hot water of 15 times, and 90 degree of lixiviates Celsius 2 hours.Part medicinal ingredient in Radix Angelicae Sinensis and the Radix Astragali can enter in Chinese medicine extract, but still can more remaining medicinal ingredients in Chinese medicine slag.
The present embodiment, fermentation mode is identical with embodiment three.First fresh plant tissue is mixed with FOS, produce after leaching sugared wine with dregs 5, then the Chinese medicine extract 4 adding cooling arrives fermentation water level 7.Chinese medicine slag 6 also together adds ferment and makes in container 1 and ferment.In order to improve the reliability of fermentation, the finished product ferment that the present embodiment adds containing dominant plant lactobacillus is inoculated.
In order to improve the extraction efficiency of traditional Chinese medicine ingredients, in step c, squeezed the juice by the solid content that fresh and alive plant tissue fermentation obtains at least 4 hours later, after getting the juice mixed culture fermentation liquid and lixiviate of squeezing out, remaining Chinese medicine slag continues fermentation.The present embodiment was preferably fermentation after 20 days, and the solid content formed the fermentation of fresh and alive plant tissue is squeezed the juice with juice extractor at a slow speed.Juice extractor refers to that with spiral propeller be the juice extractor of breaker at a slow speed, and usual rotating speed is less than 300 revs/min.The present embodiment selects rotating speed to be the vertical Normal juice machine of 70 revs/min.The crude fibre extruded of squeezing the juice is abandoned, and only leaves isolated juice and refunds the ferment making container 1 continuation fermentation that zymotic fluid and Chinese medicine slag 6 are housed.The extraction time of Chinese medicine slag 6 can be extended like this, obtain more Effective Component of Chinese Medicine.
As an alternative scheme.Ferment, after 20 days, is made all solid content high speed juice extractors in container 1 and carries out fragmentation and be stirred in zymotic fluid by anaerobic fermentation.Supernatant is got after precipitation.In above-mentioned supernatant, add light-coloured vinegar 120 milliliters, namely obtain the Chinese medicine ferment being easy to preserve.This ferment has the medicinal efficacy for the treatment of acute tubular necrosis and chronic nephritis.
In the 5th embodiment shown in Fig. 6, ferment makes container 1 and selects conventional seals container.Brown sugar selected by sugaring condiment 3.Apple selected by ferment raw material 2.The root of kudzu vine selected by Chinese medicine.
In step a, be chemical composition of Chinese materia medica by traditional Chinese medicine extraction, then dissolve or be suspended in water and form Chinese medicine extract.In the present embodiment, root of kudzu vine second alcohol and water heating lixiviate, then be separated the solid-state Puerarin of acquisition.Above-mentioned dry powder Puerarin is pressed 1:20 and water stirs, obtain the Chinese medicine extract 4 of suspendible.
By 1 kilogram of brown sugar, 3 kilograms of apples, the Chinese medicine extract 4 of 10 kilograms of suspendibles loads in the airtight container of 20 liters.After the cloth Laplace saccharomycete of inoculation gross mass 1%, carry out aerobic fermentation.Leaving a blank more than in the airtight container of 1/4, about 7 liters residual air provide enough oxygen can to cloth Laplace saccharomycete. and carry out stirring every day and can also bring more oxygen into, to ensure the aerobic quick metabolism and growth of cloth Laplace saccharomycete.In order to improve Puerarin dissolving in zymotic fluid, in step c, adding 50 degree of white wine 300 milliliters and fermenting.
After the fermentation of more than 3 months, leach zymotic fluid, namely obtain the Chinese medicine ferment with blood pressure reduction effect.The zymotic fluid leached can also add vinegar and sucrose carries out seasoning.The present embodiment, be preferably in steps d, during separation and fermentation liquid, add vinegar 800 milliliters, 1100 grams, rock sugar carries out seasoning.
Claims (10)
1. a preparation method for Chinese medicine ferment, loads ferment raw material in ferment making container and sugaring condiment ferments, and it is characterized in that comprising the following steps:
A. by least one prepared slices of Chinese crude drugs heating lixiviate at least one times, Chinese medicine extract is obtained,
B. traditional Chinese medicine extraction liquid temp described in step a is controlled to less than 50 degree Celsius,
C. by Chinese medicine extract described in step b and ferment raw material and sugaring condiment loading ferment making container, ferment more than at least 4 hours, above-mentioned ferment raw material comprises the fresh and alive plant tissue of at least one, and fermentation start time zymotic fluid acetic acid concentration lower than 1%, and fermentation start time zymotic fluid concentration of alcohol lower than 10%
D., after fermentation described in completing steps c, separate ferment makes the liquid in container.
2. the preparation method of Chinese medicine ferment according to claim 1, is characterized in that: in step c, and ferment makes after container loads Chinese medicine extract and ferment raw material and sugaring condiment, and when starting to ferment, the osmotic pressure of zymotic fluid controls between 0.5MPa to 5.1MPa.
3. the preparation method of Chinese medicine ferment according to claim 1, is characterized in that: in step c, and ferment raw material mixes with sugaring condiment and produces after weight is no less than the leaching sugar wine with dregs of sugaring condiment 30%, then adds Chinese medicine extract.
4. the preparation method of the Chinese medicine ferment according to any one of claim 1-3, is characterized in that: in step a, is immersed in by the prepared slices of Chinese crude drugs in water, is ground into Chinese medicine extract with high speed juice extractor.
5. the preparation method of the Chinese medicine ferment according to any one of claim 1-3, is characterized in that: in step c, inoculated fungi or bacterium are to zymotic fluid.
6. the preparation method of the Chinese medicine ferment according to any one of claim 1-3, is characterized in that: in steps d, is squeezed the juice by the solid content after fermentation, gets the juice of squeezing out and mix with zymotic fluid.
7. the preparation method of the Chinese medicine ferment according to any one of claim 1-3, is characterized in that: in step a, is chemical composition of Chinese materia medica by traditional Chinese medicine extraction, then dissolves or be suspended in water and form Chinese medicine extract.
8. the preparation method of the Chinese medicine ferment according to any one of claim 1-3, is characterized in that: in step c, Chinese medicine slag remaining after lixiviate is added ferment making container and together ferments.
9. the preparation method of the Chinese medicine ferment according to Claim 8 described in any one, it is characterized in that: in step c, squeezed the juice by the solid content that fresh and alive plant tissue fermentation obtains at least 4 hours later, after getting the juice mixed culture fermentation liquid and lixiviate of squeezing out, remaining Chinese medicine slag continues fermentation.
10. the preparation method of the Chinese medicine ferment according to any one of claim 1-3, is characterized in that: in step c or steps d, adds vinegar or wine to zymotic fluid.
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| CN104886414A (en) * | 2015-06-30 | 2015-09-09 | 卞洪锋 | Stabilizing agent for fruit enzyme fermentation, and preparation method and application of stabilizing agent |
| CN104958553A (en) * | 2015-07-08 | 2015-10-07 | 曾德锋 | Traditional Chinese medicine ointment for treating sore toxins and preparation method thereof |
| CN105287888A (en) * | 2015-11-02 | 2016-02-03 | 广西金臣科技有限公司 | Cherry tomato ferment nutrient solution and preparation method thereof |
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| CN104886414A (en) * | 2015-06-30 | 2015-09-09 | 卞洪锋 | Stabilizing agent for fruit enzyme fermentation, and preparation method and application of stabilizing agent |
| CN104872681A (en) * | 2015-06-30 | 2015-09-02 | 卞洪锋 | Apple microorganism ferment and preparation method thereof |
| CN104958553A (en) * | 2015-07-08 | 2015-10-07 | 曾德锋 | Traditional Chinese medicine ointment for treating sore toxins and preparation method thereof |
| CN105287888A (en) * | 2015-11-02 | 2016-02-03 | 广西金臣科技有限公司 | Cherry tomato ferment nutrient solution and preparation method thereof |
| CN105533527A (en) * | 2015-12-09 | 2016-05-04 | 珠海横琴康泽生物科技有限公司 | Prepared food using smallanthus sonchifolius fruits as raw material and preparation method thereof |
| CN106954843A (en) * | 2016-01-11 | 2017-07-18 | 徐建阳 | A kind of composite plant enzyme stoste, feature composite plant enzyme stoste and preparation method thereof and functional food |
| CN105852112A (en) * | 2016-04-29 | 2016-08-17 | 南宁市富久信息技术有限公司 | Traditional Chinese medicine health maintaining enzyme and preparation method thereof |
| JP2019514424A (en) * | 2016-04-29 | 2019-06-06 | 長沙組学生物科技有限公司Ｃｈａｎｇｓｈａ Ｏｍｉｃｓ Ｂｉｏ−Ｔｅｃｈ Ｃｏ． Ｌｔｄ | Microbial fermentation of plant chemicals |
| CN105995329A (en) * | 2016-05-23 | 2016-10-12 | 辛庆忠 | Medicinal and edible traditional Chinese medicine-containing enzyme drink and preparation method thereof |
| CN105996008A (en) * | 2016-06-24 | 2016-10-12 | 苏剑锋 | Enzyme product containing ginseng and polygonatum sibiricum and preparation method of enzyme product |
| CN106235338A (en) * | 2016-08-23 | 2016-12-21 | 广西创瑞生物科技开发有限公司 | A kind of enzyme nutrient solution and preparation method thereof |
| CN106722986A (en) * | 2016-12-05 | 2017-05-31 | 重庆市金甲农业开发有限公司 | The method that selenium-rich animal byproduct produces active bio element functional food |
| CN108542957A (en) * | 2018-04-25 | 2018-09-18 | 惠州嘉联生物科技开发有限公司 | A kind of composite enzyme and preparation method thereof with antihypertensive function |
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