CN107048361A - A kind of big rue granatum composite enzyme of jujube and preparation method thereof - Google Patents
A kind of big rue granatum composite enzyme of jujube and preparation method thereof Download PDFInfo
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- CN107048361A CN107048361A CN201710235971.8A CN201710235971A CN107048361A CN 107048361 A CN107048361 A CN 107048361A CN 201710235971 A CN201710235971 A CN 201710235971A CN 107048361 A CN107048361 A CN 107048361A
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- jujube
- granatum
- acid bacteria
- enzyme
- slurries
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- 240000008866 Ziziphus nummularia Species 0.000 title 1
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- 241000894006 Bacteria Species 0.000 claims abstract description 56
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 52
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- 239000002002 slurry Substances 0.000 claims abstract description 32
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- 239000004310 lactic acid Substances 0.000 claims abstract description 26
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 26
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 23
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- GXMWXESSGGEWEM-UHFFFAOYSA-N isoquercitrin Natural products OCC(O)C1OC(OC2C(Oc3cc(O)cc(O)c3C2=O)c4ccc(O)c(O)c4)C(O)C1O GXMWXESSGGEWEM-UHFFFAOYSA-N 0.000 description 1
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- OVSQVDMCBVZWGM-QSOFNFLRSA-N quercetin 3-O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-QSOFNFLRSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses big rue granatum composite enzyme of a kind of jujube and preparation method thereof, comprise the following steps：It is 1 that jujube, granatum and big rue, which are added water according to material-water ratio,:3~5 are squeezed the juice or defibrination, obtain slurries；Mixed using pectase, cellulase and zytase as complex enzyme with slurries, enzymolysis obtains enzymolysis liquid in 3~4 hours at 50~60 DEG C；The addition quality of the complex enzyme is the 0.2%~0.6% of the grind slurries quality；It is used as with saccharomycete, lactic acid bacteria and acetic acid bacteria in seed liquor, access enzymolysis liquid, cut-in quality is the 2~4% of the enzymolysis liquid weight, ferment 50~60d at 28~32 DEG C, obtains zymotic fluid；The zymotic fluid is concentrated, the big rue granatum composite enzyme of jujube is obtained.Without any chemical substance or additive in whole manufacturing process, the multiple health cares such as the beneficial menses of fatigue, kidney-replenishing are resisted strenuously with preferably anti-oxidant anti-aging, enhancing immunity of organisms, suppression cancer anti-cancer, reinforcement.
Description
Technical field
The present invention relates to ferment, more particularly to a kind of big rue granatum composite enzyme of jujube and preparation method thereof.
Background technology
Ferment uses the pure plants such as various plants, vegetables and fruits, seed, herbal medicine, marine alga to be prepared for raw material.The main component of ferment
In addition to multiple eating ferment, there are trace element, mineral matter, vitamin, amino acid, protein, carbohydrate etc., with pure
Natural feature.But, the plant material such as plant, vegetables and fruits, seed, herbal medicine of selection is differed, and specific preparation method is because of producer
Different, its main component is also variant.
Herba Cistanches also known as golden bamboo shoot, smart, desert cistanche, big rue, be doing for orobanchaceae plant cistanche or Cistanche tubulosa
The dry fleshy stem with scale leaf.《Pharmacopeia》Saline cistanche is recorded, it is sweet, salty, it is warm-natured, there is a kidney-replenishing, beneficial menses, the work(such as relax bowel
Effect, for soreness and weakness of waist and knees, muscles and bones is powerless, impotence, female sterility, dry constipation of intestines etc..Its chemical composition mainly have benzyl carbinol glycoside,
Iridoid glycosides, lignin, amino acid, carbohydrate, hydro carbons, alkaloid, flavonoids, PEARLITOL 25C, β-paddy steroid, alcohol etc..It is existing
Also confirm that saline cistanche in addition to tonifying kidney and strengthening yang, the effect relaxed bowel for pharmacological research, also with antifatigue, anti-aging,
Strengthen the effect such as immunity of organisms and enhancing memory.Multiple efficacies based on saline cistanche, further carry out active component and
On the basis of study of active components, saline cistanche is expected to be developed into improving sexual function, anti-senile dementia, liver protection, defaecation, tumour auxiliary
The medicine of the effects such as treatment, radioresistance, while can be developed into sportsman strengthens physical, antifatigue and the labor that delays to decline health products.
Granatum is the drying pericarp of pomegranate, contains many kinds of substance and amino acid.Granatum containing tannin 10.4~21.3%,
Wax 0.8%, resin 4.5%, mannitol 1.8%, sugar 2.7%, natural gum 3.2%, inulin 1.0%, cement 0.6%, gallic acid
4.0%th, malic acid, pectin, calcium oxalate, Isoquercitrin and granatenine etc..The amino acid that granatum contains be asparatate,
About 0.97g in threonine, serine, glutamic acid, glycine etc., wherein content of glutamic acid highest, every 100g granatums, next to that
Histidine.Also multiple element is contained in granatum.In terms of pharmacological action, granatum acid, puckery with the hemostasis of puckery intestines, are killed
The effect of worm.Primary treatment bacillary dysentery, amoebic dysentery and multi-infection disease.Tannin in granatum can coagulate because of it
Gu the plasm in microbial body and the influence to a variety of enzymes, have inhibitory action to various bacteria, fungi, virus, also with exempting from
Epidemic disease regulation has effect.
The research about ferment is primarily present following a few point defects in the prior art：1) raw material does not handle direct fermentation utilization
Rate is low, and enzyme activity material recovery rate is low.2) pure-blood ferment strain metabolite function is single, no characteristic；Fermentation method is used
Traditional natural microflora fermentation, modern compounding pure-blood ferment or the combination of the two, excessively traditional, no innovation；3) ferment biological activity
And stability is poor, the shelf-life is short；4) taste and flavor bad adaptability, appetite are low.
It there is no the technique study for preparing composite enzyme as raw material using the big rue of jujube and granatum at present.
The content of the invention
In view of this, the invention provides big rue granatum composite enzyme of a kind of jujube and preparation method thereof, biology is utilized
Enzyme is degraded to raw material, is fermented using different fermentations strain, improves the utilization rate of fermentation.In whole process without appoint
The bioactivity loss of what chemical composition and additive, farthest reduction ferment finished product, makes ferment be sent out after human intake
Wave its maximum activity.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The invention provides a kind of preparation method of the big rue granatum composite enzyme of jujube, comprise the following steps：
(1) 6~8 parts by weight jujubes, 2~3 parts by weight granatums and the big rue of 0.5~1 parts by weight are added water according to material-water ratio
For 1:3~5 are squeezed the juice or defibrination, obtain slurries；
(2) using pectase, cellulase and zytase as complex enzyme, mixed with step (1) described slurries, 50~
Enzymolysis obtains enzymolysis liquid in 3~4 hours at 60 DEG C；
The addition quality of the complex enzyme is the 0.2%~0.6% of the grind slurries quality；
(3) it is used as with saccharomycete, lactic acid bacteria and acetic acid bacteria in seed liquor, access step (2) described enzymolysis liquid, cut-in quality
For the 2~4% of the enzymolysis liquid weight, ferment 50~60d at 28~32 DEG C, obtains zymotic fluid；Described in the seed liquor
The total concentration of saccharomycete, the lactic acid bacteria and the acetic acid bacteria is 105~108CFU/mL；
(4) step (3) zymotic fluid is concentrated, obtains the big rue granatum composite enzyme of jujube.
It is preferred that, the weight ratio of the pectase, the cellulase and the zytase is 2~4：3~5：1~3.
It is preferred that, the weight ratio of the saccharomycete, the lactic acid bacteria and the acetic acid bacteria is 0.8~1.2：0.4~0.6：
0.4~0.6.
It is further preferred that the saccharomycete is the one or more in brewer's yeast, Saccharomyces cerevisiae and wine yeast.
It is further preferred that the lactic acid bacteria is Lactobacillus plantarum.
It is furthermore preferred that the deposit number of the Lactobacillus plantarum is CCTCC No：M 2015453.
It is preferred that, in step (4), the condition of the concentration is：By step (3) zymotic fluid vacuum 0.05~
Cryogenic vacuum is concentrated at 0.12MPa, 40~45 DEG C of temperature.
It is further preferred that it is described concentration in a water bath rotation carry out, by the zymotic fluid be evaporated to original volume 1/2~
1/3, obtain the big rue granatum composite enzyme of jujube.
It is preferred that, the jujube is precooked and is enucleated using preceding.
Present invention additionally comprises the big rue granatum composite enzyme of the jujube prepared by the above method.
Compared with prior art, the present invention has advantages below to the present invention：
(1) present invention is prepared compound using jujube, big rue and granatum as raw material using the method for enzymolysis and microbial fermentation
Ferment.Without any chemical substance or additive in whole manufacturing process, the method fermented using biological enzymolysis obtains height
The ferment of bioactivity.
(2) present invention uses pectase, cellulase and zytase by the macromolecular complex in jujube, big rue and granatum
Matter is decomposed, and obtained enzymolysis liquid is more beneficial for the fermentation of microorganism, improves fermentation efficiency.
(3) present invention is fermented using multiple strains of saccharomycete, lactic acid bacteria and acetic acid bacteria to enzymolysis liquid, metabolite
It is various, make to contain multiple beneficial composition in the ferment that fermentation obtains, healthcare function is various, taste and flavor is enriched, and adaptability is good.
(4) composite enzyme of the invention has the healthcare function of three kinds of raw material concurrently, and is passing through complex enzyme hydrolysis and micro- life
After thing fermentation, macromolecular components are transformed into small molecule active composition, exempted from preferably anti-oxidant anti-aging, enhancing body
Epidemic disease power, suppression cancer anti-cancer, reinforcement resist the multiple health cares such as the beneficial menses of fatigue, kidney-replenishing strenuously.
(5) granatum is the accessory substance of pomegranate processing, and Determination of Polyphenols reaches 10%.The present invention is deep exploitation pomegranate
Skin, rationally using this part waste resource, granatum is used to produce plant enzyme, so as to be the profit of granatum medical value
Foundation is provided with, the exploitation of Related product and standardized production.
Biological deposits explanation
Lactobacillus plantarum Lactobacillusplantarum, Chinese Typical Representative culture is preserved on July 14th, 2015
Collection, address is Wuhan, China Wuhan University, and bacterial strain deposit number is CCTCC No：M 2015453.
Brief description of the drawings
Fig. 1 is pectase addition single factor experiment result；
Fig. 2 is cellulose addition single factor experiment result；
Fig. 3 is zytase addition single factor experiment result；
Fig. 4 is hydrolysis temperature single factor experiment result；
Fig. 5 is enzymolysis time single factor experiment result；
Fig. 6 is solid-liquid ratio single factor experiment result.
Embodiment
The invention provides the preparation method of the big rue granatum composite enzyme of jujube, comprise the following steps：
(1) 6~8 parts by weight jujubes, 2~3 parts by weight granatums and the big rue of 0.5~1 parts by weight are added water according to material-water ratio
For 1:3~5 are squeezed the juice or defibrination, obtain slurries；
(2) using pectase, cellulase and zytase as complex enzyme, mixed with step (1) described slurries, 50~
Enzymolysis obtains enzymolysis liquid in 3~4 hours at 60 DEG C；
The addition quality of the complex enzyme is the 0.2%~0.6% of the grind slurries quality；
(3) it is used as with saccharomycete, lactic acid bacteria and acetic acid bacteria in seed liquor, access step (2) described enzymolysis liquid, cut-in quality
For the 2~4% of the enzymolysis liquid weight, ferment 50~60d at 28~32 DEG C, obtains zymotic fluid；Described in the seed liquor
The total concentration of saccharomycete, the lactic acid bacteria and the acetic acid bacteria is 105~108CFU/mL；
(4) step (3) zymotic fluid is concentrated, obtains the big rue granatum composite enzyme of jujube.
The present invention prepares composite enzyme using jujube, big rue and granatum as raw material, makes to contain in the composite enzyme prepared
There are jujube, big rue and granatum and effect, increase the health care scope of ferment.The present invention using jujube as primary raw material,
Granatum of the part frequently as processing byproduct and the big rue of the Chinese medicine with various health care functions are with the addition of, composite enzyme is improved
In health-care components.
In the present invention, the addition of jujube is 6~8 parts by weight, preferably 7 parts by weight.Source of the present invention to jujube
There is no special restriction with kind, using commercially available jujube.In embodiments of the present invention, hotan jujube is used,
It is sweet and delicious tasty, nutritious, per 100g fresh dates edible parts sugar content 24%, also containing protein, fat and calcium, phosphorus,
Mineral element and multivitamin, especially Vc content are up to 540mg per 100g fresh dates edible parts necessary to the human bodies such as iron,
It is higher than citrus 7~10 times much higher than apple 100 times, the effects such as with blood-nourishing invigorating the spleen, tonifying five zang organs, governance wasting, to hypertension, liver
A variety of diseases such as hardening have good curative effect.
The present invention is digested for the ease of jujube, after preferably jujube is precooked, stoning.The present invention first will be fresh
Jujube is cleaned with circulating water, removes the impurity such as the soil of adhesion.The method that the present invention is precooked and is enucleated to jujube does not have special
Limit, jujube is precooked using the conventional method of this area, reach the degree of convenient stoning.In the specific embodiment of the invention
In, jujube is precooked in 100 DEG C of water, the time of precooking, for 10~20min, is further 13~17min.By the jujube after precooking
Stoning.The present invention is not particularly limited to the mode of stoning, can be using stoning by hand or machinery stoning.Jujube after stoning is used
In squeezing the juice or defibrination.
Present invention addition granatum is 2~3 parts by weight, preferably 2.2~2.8 parts by weight.The present invention comes to granatum
Source and pomegranate kind are not particularly limited, can be using the processing byproduct for purchasing pomegranate product on the market.Effect of granatum
Come from the polyphenols in fruit, wherein being enriched the most with content in pericarp.Granatum accounts for the 20%~30% of pomegranate, mesh
It is preceding except minority it is medicinal in addition to, go out of use mostly.Therefore, this living resources is developed, with important theory significance and reality
Application value.In the specific embodiment of the invention, granatum is preferred to use the pomegranate kind of Xinjiang plantation.The stone that the present invention is applied
Granatum is preferably broken into after fritter by pomegranate skin, is used further to squeeze the juice or defibrination.
Big rue addition of the invention is 0.5~1 parts by weight, is further 0.7~0.8 parts by weight.Based on many of big rue
Kind of effect, it is expected to be developed into improve sexual function, anti-senile dementia, liver protection, defaecation, tumor aid treatment, radioresistance etc. many
The medicine of kind of effect, while can be developed into sportsman strengthens physical, antifatigue and the labor that delays to decline health products.Big rue can be eaten with medicine
It is general, not only delicious flavour, uniqueness, and have nourishing function concurrently.Big rue is preferably broken into after fritter by the present invention, is used further to squeeze the juice
Or defibrination.
After the present invention adds water jujube, granatum and big rue, squeezed the juice or defibrination, starched according to certain material-water ratio
Liquid.The present invention is not particularly limited to the equipment squeezed the juice, and is squeezed the juice using commercially available juice extractor.The present invention can also be by original
Material carries out defibrination and obtains slurries.Squeeze the juice or defibrination during can not substantially observe that raw material block as principle prepares slurries using naked eyes.
Obtained slurries are used for the enzymolysis of complex enzyme.By jujube, big rue and granatum squeeze the juice or defibrination after obtain slurries, be conducive to increase
The contact area of raw material and complex enzyme, enables raw material fully to be contacted with complex enzyme, enzymolysis process is more thorough.
The present invention is digested slurries obtained above with complex enzyme.Complex enzyme in the present invention refers to containing pectin
The complex enzyme of enzyme, cellulase and zytase.The present invention carries out single factor experiment and multifactorial experiment to complex enzyme zymohydrolysis, with
It is determined that optimal enzymatic hydrolysis condition.
Complex enzyme hydrolysis single factor experiment is as follows：
(1) pectin enzyme dosage：In 50 DEG C of hydrolysis temperature, enzymolysis time 4h, raw material:Water=1:Under conditions of 3, using difference
Enzyme concentration (0.1%, 0.2%, 0.3%, 0.4%, 0.5%) extraction of the juice, influence of the research enzyme concentration to extraction of the juice effect.As a result table
It is bright：Soluble solid content reaches maximum in enzymolysis liquid when pectase addition is 0.3%；
(2) cellulase consumption：In 50 DEG C of hydrolysis temperature, enzymolysis time 4h, raw material:Water=1:Under conditions of 3, using not
Same enzyme concentration (0.1%, 0.2%, 0.3%, 0.4%, 0.5%) extraction of the juice, influence of the research enzyme concentration to extraction of the juice effect.As a result
Show：Soluble solid content when cellulase addition is 0.4% in enzymolysis liquid reaches maximum.
(3) zytase consumption：In 50 DEG C of hydrolysis temperature, enzymolysis time 4h, raw material:Water=1:Under conditions of 3, using not
Same enzyme concentration (0.1%, 0.2%, 0.3%, 0.4%, 0.5%) extraction of the juice, influence of the research enzyme concentration to extraction of the juice effect.As a result
Show：Soluble solid content when zytase addition is 0.2% in enzymolysis liquid reaches maximum.
(4) hydrolysis temperature：In enzymolysis time 4h, pectase 0.3%, cellulase 0.4%, zytase 0.2%, original
Material:Water=1:Under conditions of 3, using different (30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C) enzymolysis of hydrolysis temperature, hydrolysis temperature pair is studied
The influence of hydrolysis result.As a result show：Soluble solid content reaches maximum in enzymolysis liquid when hydrolysis temperature is 50 DEG C.
(5) enzymolysis time：It is former in 50 DEG C of hydrolysis temperature, pectase 0.3%, cellulase 0.4%, zytase 0.2%
Material:Water=1:Under conditions of 4, digested using different enzymolysis times (1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h), during research enzymolysis
Between influence to hydrolysis result.As a result show：Enzymolysis time reaches most for soluble solid content in 4~5 hours enzymolysis liquids
Big value；
(6) solid-liquid ratio：In 50 DEG C of hydrolysis temperature, enzymolysis time 4h, pectase 0.3%, cellulase 0.4%, xylan
Under conditions of enzyme 0.2%, enzymolysis examination is carried out with the water that addition is respectively 2 times, 3 times, 4 times, 5 times, 6 times and 7 times of raw material weight
Test, study influence of the amount of water to hydrolysis result.As a result show：Solid-liquid ratio is 1:Soluble solid contains in enzymolysis liquid when 4~5
Amount is of a relatively high, and enzymolysis liquid viscosity is preferable.
Complex enzyme hydrolysis orthogonal test is as follows：
From L4(32) orthogonal arrage progress three factor two horizontal quadrature experiment, determine Optimizing Technical.With soluble solid
Thing, reduced sugar and total sugar content are test index.
The complex enzyme addition orthogonal test factor level table of table 1
From L9(43) orthogonal arrage progress four factor three horizontal quadrature experiment, determine Optimizing Technical.With soluble solid
Thing, reduced sugar and total sugar content are test index.(factor level is shown in Table 2)
The complex enzyme hydrolysis orthogonal test factor level of table 2
The complex enzyme hydrolysis orthogonal experiments of table 3
It is No. 9 experiments, i.e. A3B3C2D1, soluble solid from the optimum combination in 3,9 groups of experiments of orthogonal test table
The variable quantity of thing content is 2.25%；Secondly it is No. 3 experiment A1B3C3D3, the variable quantity of soluble solid content is
1.25%.According to extreme difference R size, the primary and secondary for determining influence factor is B>A>D>C, optimum proportioning is A3B3C2D1, that is, is mixed
The addition of enzyme is 0.48%, and hydrolysis temperature is 55 DEG C, and enzymolysis time is 4h, and solid-liquid ratio is 1:3.
Experiment draws currently preferred enzymatic hydrolysis condition more than：
The present invention, which adds water jujube, granatum and big rue, to be squeezed the juice or defibrination, obtains slurries.The material-water ratio of the slurries
For 1:3~5, more preferably 1:3.5~4.5.In complex enzyme of the present invention, pectase, cellulase and zytase
Weight ratio preferably 2~4：3~5：1~3 has preferable hydrolysis result.Carried out after the complex enzyme is mixed with the slurries
Fermentation.In the present invention, the addition of the complex enzyme be the grind slurries quality 0.2%~0.6%, preferably 0.3%~
0.5%.The hydrolysis temperature of the present invention is 50~60 DEG C, preferably 54~58 DEG C.Enzymolysis time is 3~4 hours, preferably 3.2
~3.7 hours.After slurries are by complex enzyme zymohydrolysis, the macromolecular substances in slurries are decomposed into and are conducive to microbial fermentation
Small-molecule substance, to improve utilization rate of the microorganism to raw material in zymotic fluid in fermentation process.
Enzymolysis liquid is prepared into zymotic fluid with microorganism seed liquid fermentation.
Microorganism used is saccharomycete, lactic acid bacteria and acetic acid bacteria in the present invention, and its weight ratio is 0.8~1.2：0.4~
0.6：0.4~0.6.Wherein, the saccharomycete is the one or more in brewer's yeast, Saccharomyces cerevisiae and wine yeast.This
Invention is not particularly limited to the source of saccharomycete, using commercially available saccharomycete.Lactic acid bacteria of the present invention is the newborn bar of plant
Bacterium, it is preferred to use the isolated bacterial strain from Xinjiang traditional national dairy produce-Yoghourt, has been preserved in Chinese Typical Representative culture
Thing collection, bacterial strain deposit number is CCTCCNo：M 2015453.The characteristics of Lactobacillus plantarum used in the present invention is extracellular
Polysaccharide yield is very high, and its yield of extracellular polysaccharide is up to 383.2 μ g/mL.Acetic acid bacteria of the present invention is acetobacter, and the present invention is right
The source of acetic acid bacteria is not particularly limited, using commercially available prod.
The process that the present invention prepares seed liquor can be prepared using the conventional method of this area.Specifically, the ferment
Female bacterium is cultivated with malt extract medium, and the lactic acid bacteria is cultivated with MRS culture mediums, and the acetic acid bacteria is trained with acetic acid bacteria
Base is supported to be cultivated.Cultivation temperature is 25~30 DEG C, preferably 26~28 DEG C.In growth after above-mentioned strain is cultivated
Logarithmic phase be enlarged culture again, the seed liquor is made.
Seed liquor containing saccharomycete, lactic acid bacteria and acetic acid bacteria mixes to access is above-mentioned to be prepared according to aforementioned proportion
Fermented in enzymolysis liquid.The total concentration of saccharomycete, the lactic acid bacteria and the acetic acid bacteria described in the seed liquor is 105~
108CFU/mL, more preferably 106~107CFU/mL；The cut-in quality of preferred seed liquid for the enzymolysis liquid weight 2~
4%, more preferably 2.5~3.5%.In the present invention, the fermentation temperature of enzymolysis liquid is 28~32 DEG C, preferably 29~31 DEG C；Hair
The ferment time is 50~60d, more preferably 54~58d.After microbial fermentation, the soluble solid content in zymotic fluid
Substantially reduced with total sugar content, content of reducing sugar rise, total acid content is 4.9~5.1g/L.
The zymotic fluid obtained after microbial fermentation is concentrated, the big rue granatum composite enzyme of jujube is obtained.In this hair
In bright, preferably concentrated under cryogenic vacuum, to retain the bioactivity in ferment to greatest extent.It is preferred that, the present invention
Cryogenic vacuum concentration condition be：Vacuum is 0.05~0.12MPa, more preferably 0.08~0.1MPa；Temperature is 40~45
DEG C, it is further 42~44 DEG C.Present invention water-bath rotary evaporation under the conditions of above-mentioned cryogenic vacuum by zymotic fluid, to zymotic fluid
Volume concentration obtains the big rue granatum composite enzyme of jujube to the 1/2~1/3 of original volume.The cryogenic vacuum concentration work of the present invention
Skill, can improve thickening efficiency and can save, also farthest save the activity of probiotics.
Present invention additionally comprises the big rue granatum composite enzyme of the jujube prepared by the above method.The ferment has three kinds concurrently
The healthcare function of raw material, and after complex enzyme hydrolysis and microbial fermentation, macromolecular components are transformed into small molecule
Active component, fatigue, kidney-replenishing are resisted strenuously with preferably anti-oxidant anti-aging, enhancing immunity of organisms, suppression cancer anti-cancer, reinforcement
The multiple health cares such as beneficial menses.
With reference to embodiment, the present invention will be described in detail, but they can not be interpreted as to present invention protection
The restriction of scope.
Embodiment 1
The new fresh dates of 6kg are cleaned with circulating water, the impurity such as the soil of adhesion is removed, jujube is put into water, heating temperature
Spend and the 16min that precooks is carried out to jujube for 100 DEG C, the jujube stoning after precooking.2kg granatums and the big rues of 0.5kg are smashed into
After fritter, poured into together with the jujube of stoning in juice extractor add water according to material-water ratio be 1:4 are beaten, and cannot make out to naked eyes
Untill obvious fruit pulp, slurries are obtained.
By pectase, cellulase and zytase according to mass ratio be 2:3:1 carries out being mixed to get complex enzyme.In slurries
The complex enzyme of middle addition grind slurries quality 0.31%, enzymolysis obtains enzymolysis liquid in 4 hours at 50 DEG C.
Saccharomycete, lactic acid bacteria and acetic acid bacteria are inoculated in culture medium respectively, cultivated at 26 DEG C, then expands culture
Seed liquor is made.Enzymolysis liquid is sterilized at 115 DEG C after 15min, the weight ratio of access saccharomycete, lactic acid bacteria and acetic acid bacteria is
0.8：0.4：The total concentration of saccharomycete, lactic acid bacteria and acetic acid bacteria is 10 in 0.4 seed liquor, seed liquor8CFU/mL, seed liquor connects
It is the 2% of the enzymolysis liquid weight to enter amount, and ferment 60d at 28 DEG C, obtains zymotic fluid.
By zymotic fluid, water-bath rotates at vacuum 0.1MPa, 40 DEG C of temperature, is evaporated to the 1/2-1/ of zymotic fluid original volume
3, obtain the big rue granatum composite enzyme of jujube.
Embodiment 2
The new fresh dates of 8kg are cleaned with circulating water, the impurity such as the soil of adhesion is removed, jujube is put into water, heating temperature
Spend and the 20min that precooks is carried out to jujube for 100 DEG C, the jujube stoning after precooking.3kg granatums and the big rues of 1kg are smashed into small
After block, poured into together with the jujube of stoning in juice extractor add water according to material-water ratio be 1:5 are beaten, and cannot make out to naked eyes bright
Untill aobvious fruit pulp, slurries are obtained.
By pectase, cellulase and zytase according to mass ratio be 4:5:3 carry out being mixed to get complex enzyme.In slurries
The complex enzyme of middle addition grind slurries quality 0.37%, enzymolysis obtains enzymolysis liquid in 4 hours at 60 DEG C.
Saccharomycete, lactic acid bacteria and acetic acid bacteria are inoculated in culture medium respectively, cultivated at 28 DEG C, then expands culture
Seed liquor is made.Enzymolysis liquid is sterilized at 115 DEG C after 15min, the weight ratio of access saccharomycete, lactic acid bacteria and acetic acid bacteria is
1.2：0.6：The total concentration of saccharomycete, lactic acid bacteria and acetic acid bacteria is 10 in 0.5 seed liquor, seed liquor7CFU/mL, seed liquor connects
It is the 4% of the enzymolysis liquid weight to enter amount, and ferment 50d at 32 DEG C, obtains zymotic fluid.
By zymotic fluid, water-bath rotates under vacuum 0.08MPa, temperature 45 C, is evaporated to the 1/2-1/ of zymotic fluid original volume
3, obtain the big rue granatum composite enzyme of jujube.
Embodiment 3
The new fresh dates of 7kg are cleaned with circulating water, the impurity such as the soil of adhesion is removed, jujube is put into water, heating temperature
Spend and the 14min that precooks is carried out to jujube for 100 DEG C, the jujube stoning after precooking.2kg granatums and the big rues of 0.6kg are smashed into
After fritter, poured into together with the jujube of stoning in juice extractor add water according to material-water ratio be 1:3 are beaten, and cannot make out to naked eyes
Untill obvious fruit pulp, slurries are obtained.
By pectase, cellulase and zytase according to mass ratio be 3:4:2 carry out being mixed to get complex enzyme.In slurries
The complex enzyme of middle addition grind slurries quality 0.48%, enzymolysis obtains enzymolysis liquid in 4 hours at 55 DEG C.
Saccharomycete, lactic acid bacteria and acetic acid bacteria are inoculated in culture medium respectively, cultivated at 30 DEG C, then expands culture
Seed liquor is made.Enzymolysis liquid is sterilized at 115 DEG C after 15min, the weight ratio of access saccharomycete, lactic acid bacteria and acetic acid bacteria is
1.0：0.5：The total concentration of saccharomycete, lactic acid bacteria and acetic acid bacteria is 10 in 0.4 seed liquor, seed liquor6CFU/mL, seed liquor connects
It is the 3% of the enzymolysis liquid weight to enter amount, and ferment 55d at 30 DEG C, obtains zymotic fluid.
By zymotic fluid, water-bath rotates at vacuum 0.09MPa, 42 DEG C of temperature, is evaporated to the 1/2-1/ of zymotic fluid original volume
3, obtain the big rue granatum composite enzyme of jujube.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of the big rue granatum composite enzyme of jujube, it is characterised in that comprise the following steps：
(1) it is 1 6~8 parts by weight jujubes, 2~3 parts by weight granatums and the big rue of 0.5~1 parts by weight to add water according to material-water ratio:3
~5 are squeezed the juice or defibrination, obtain slurries；
(2) using pectase, cellulase and zytase as complex enzyme, mixed with step (1) described slurries, at 50~60 DEG C
Lower enzymolysis obtains enzymolysis liquid in 3~4 hours；
The addition quality of the complex enzyme is the 0.2%~0.6% of the grind slurries quality；
(3) it is used as with saccharomycete, lactic acid bacteria and acetic acid bacteria in seed liquor, access step (2) described enzymolysis liquid, cut-in quality is institute
The 2~4% of enzymolysis liquid weight are stated, ferment 50~60d at 28~32 DEG C, obtains zymotic fluid；Yeast described in the seed liquor
The total concentration of bacterium, the lactic acid bacteria and the acetic acid bacteria is 105~108CFU/mL；
(4) step (3) zymotic fluid is concentrated, obtains the big rue granatum composite enzyme of jujube.
2. according to the method described in claim 1, it is characterised in that the pectase, the cellulase and the xylan
The weight ratio of enzyme is 2~4：3~5：1~3.
3. according to the method described in claim 1, it is characterised in that the saccharomycete, the lactic acid bacteria and the acetic acid bacteria
Weight ratio is 0.8~1.2：0.4~0.6：0.4~0.6.
4. the method according to claim 1 or 3, it is characterised in that the saccharomycete is brewer's yeast, Saccharomyces cerevisiae and Portugal
One or more in grape brewer yeast.
5. the method according to claim 1 or 3, it is characterised in that the lactic acid bacteria is Lactobacillus plantarum.
6. method according to claim 5, it is characterised in that the deposit number of the Lactobacillus plantarum is CCTCC No：M
2015453。
7. according to the method described in claim 1, it is characterised in that in step (4), the concentration is：By step (3) hair
Zymotic fluid cryogenic vacuum at 0.05~0.12MPa of vacuum, 40~45 DEG C of temperature is concentrated.
8. method according to claim 7, it is characterised in that the concentration rotates progress in a water bath, by the fermentation
Liquid is evaporated to the 1/2~1/3 of original volume, obtains the big rue granatum composite enzyme of jujube.
9. according to the method described in claim 1, it is characterised in that the jujube is precooked and is enucleated using preceding.
10. the big rue granatum composite enzyme of the jujube prepared according to claim 1~9 any one methods described.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN114176216A (en) * | 2020-09-14 | 2022-03-15 | 赵善廷 | Preparation method of multi-strain step-by-step fermented red date enzyme |
| CN114958946A (en) * | 2022-03-09 | 2022-08-30 | 山西勰成生物科技有限公司 | Preparation method of fermented red date chelated peptide iron |
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