CN105831745A - A method of preparing golden silk jujube ferment - Google Patents
A method of preparing golden silk jujube ferment Download PDFInfo
- Publication number
- CN105831745A CN105831745A CN201610222350.1A CN201610222350A CN105831745A CN 105831745 A CN105831745 A CN 105831745A CN 201610222350 A CN201610222350 A CN 201610222350A CN 105831745 A CN105831745 A CN 105831745A
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- Prior art keywords
- ferment
- golden jujube
- enzymolysis
- temperature
- enzyme
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- 238000000034 method Methods 0.000 title claims abstract description 11
- 240000008866 Ziziphus nummularia Species 0.000 title 1
- 241001247821 Ziziphus Species 0.000 claims abstract description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- 229930006000 Sucrose Natural products 0.000 claims abstract description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 12
- 238000007710 freezing Methods 0.000 claims abstract description 7
- 239000002253 acid Substances 0.000 claims abstract description 4
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 5
- 238000010009 beating Methods 0.000 claims description 4
- 230000007159 enucleation Effects 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 3
- 238000006460 hydrolysis reaction Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 239000002304 perfume Substances 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 2
- 239000011592 zinc chloride Substances 0.000 claims description 2
- 235000005074 zinc chloride Nutrition 0.000 claims description 2
- 239000004382 Amylase Substances 0.000 abstract description 2
- 102000013142 Amylases Human genes 0.000 abstract description 2
- 108010065511 Amylases Proteins 0.000 abstract description 2
- 102000004882 Lipase Human genes 0.000 abstract description 2
- 239000004367 Lipase Substances 0.000 abstract description 2
- 108090001060 Lipase Proteins 0.000 abstract description 2
- 108091005804 Peptidases Proteins 0.000 abstract description 2
- 239000004365 Protease Substances 0.000 abstract description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 2
- 235000019418 amylase Nutrition 0.000 abstract description 2
- 229930003935 flavonoid Natural products 0.000 abstract description 2
- 150000002215 flavonoids Chemical class 0.000 abstract description 2
- 235000017173 flavonoids Nutrition 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 2
- 235000019421 lipase Nutrition 0.000 abstract description 2
- 239000011707 mineral Substances 0.000 abstract description 2
- 235000010755 mineral Nutrition 0.000 abstract description 2
- 150000002989 phenols Chemical class 0.000 abstract description 2
- 229930000044 secondary metabolite Natural products 0.000 abstract description 2
- 239000011782 vitamin Substances 0.000 abstract description 2
- 235000013343 vitamin Nutrition 0.000 abstract description 2
- 229930003231 vitamin Natural products 0.000 abstract description 2
- 229940088594 vitamin Drugs 0.000 abstract description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 abstract 1
- 108010012715 Superoxide dismutase Proteins 0.000 abstract 1
- 150000007513 acids Chemical class 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000000490 cosmetic additive Substances 0.000 abstract 1
- 239000002778 food additive Substances 0.000 abstract 1
- 239000003205 fragrance Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 235000020418 red date juice Nutrition 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 238000009777 vacuum freeze-drying Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 229960000583 acetic acid Drugs 0.000 description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A method of preparing golden silk jujube ferment is disclosed. The method includes (1) cleaning golden silk jujubes, and separating through centrifuging to obtain jujube juice, (2) performing composite enzymolysis, (3) blending after the enzymolysis, (4) stirring, feeding air, and fermenting, (5) supplementing a 5% cane sugar solution, feeding air, and fermenting for 24 h to generate acids and fragrance, (6) inoculating a 10% acetic acid bacteria seed solution and (7) quick-freezing the fermented substance at -40 DEG C, adding into a vacuum freeze-drying bin, and performing freeze-drying to obtain the ferment the water content of which is less than 5%. The ferment prepared by the method contains rich protease, lipase, superoxide dismutase, amylase, vitamins and mineral compounds, and contains secondary metabolites such as phenols and flavonoids. The ferment has a wide application prospect as a novel food and cosmetic additive in the market.
Description
Technical field
The present invention relates to foodstuff production method, particularly to a kind of method preparing Golden jujube ferment.
Background technology
The dry Fructus Jujubae of Golden jujube through rehydration, making beating peeling enucleation, catch juice after remaining usually used as feedstuff
Selling, be worth little, if being further processed into ferment, its increment amplitude is huge.
Summary of the invention
It is an object of the invention to: a kind of method preparing Golden jujube ferment.
The present invention is achieved in that a kind of method preparing Golden jujube ferment of the present invention, its feature
Be: it as follows:
(1) Golden jujube dry fruit is cleaned, rehydration, after making beating peeling enucleation, be performing centrifugal separation on obtaining
Fructus Jujubae juice;
(2) complex enzyme hydrolysis: pH is 5.5, and temperature is 55 DEG C, and enzymolysis time is 180min;With step
Suddenly (1) adds the weight meter of Golden jujube dry fruit, and xylanase, the enzyme concentration of glucanase are respectively
500U/g, the enzyme concentration of Novi's letter enzyme and cellulase is respectively 800U/g and 400U/g;
(3) allocate after enzymolysis, add white sugar and Mel so that mixed liquor soluble solid
Content is 17%-23%;Based on step (2) product, add yeast extract 0.2%, ammonium sulfate 1.9%, chlorine
Change zinc 0.1%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.6%, high temperature sterilize 15min at 121 DEG C;
(4) 31 DEG C of entrance fermentation tanks it are cooled to;Based on the weight of step (3) product, inoculation yeast
Bacterium, inoculum concentration: 1% (, in fermentation tank, it is passed through filtrated air, stirs aerobic fementation 12h;
(5) sucrose solution of 5%, based on step (3) product, inoculating lactic acid bacterium 0.025g/L are supplemented;
5% sucrose solution activation 40min, temperature 37 DEG C, aerobic fementation 24h produces acid and produces perfume;
(6) 10% acetic acid bacteria seed liquor is inoculated, 32 DEG C, aerobic fementation 10h, consume ethanol;10% vinegar
Acid bacterium seed liquor i.e. concentration is the acetic acid bacteria seed liquor of 10%
(7) fermented product after fermentation, the quick-freezing of-40 DEG C, enters back into vacuum freezing drying cabin and carries out
Lyophilization, finally gives the moisture Golden jujube ferment less than 5%.
The Golden jujube ferment that the inventive method is produced contains abundant protease, lipase, super oxidation
Thing dismutase, amylase, vitamin, mineral and the secondary metabolite such as phenols, flavonoid.
Golden jujube ferment is as the novel food in market and cosmetics additive, and application prospect is boundless.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
A kind of method preparing Golden jujube ferment, it as follows:
(1) Golden jujube dry fruit is cleaned, rehydration, after making beating peeling enucleation, be performing centrifugal separation on obtaining
Fructus Jujubae juice;
(2) complex enzyme hydrolysis: pH is 5.5, and temperature is 55 DEG C, and enzymolysis time is 180min;Wood is poly-
Carbohydrase, the enzyme concentration of glucanase are respectively 500U/g, and the enzyme concentration of Novi's letter enzyme and cellulase divides
Wei 800U/g and 400U/g;By in terms of the weight that step (1) adds Golden jujube dry fruit;
(3) allocate after enzymolysis, add white sugar and Mel so that mixed liquor soluble solid
Content is 17%, adds yeast extract 0.2%, ammonium sulfate 1.9%, zinc chloride 0.1%, magnesium sulfate 0.1%,
Potassium dihydrogen phosphate 0.6%, high temperature sterilize 15min at 121 DEG C;Based on step (2) product;
(4) being cooled to 31 DEG C of entrance fermentation tanks, inoculation yeast bacterium, inoculum concentration: 1%, in fermentation tank
It is passed through filtrated air, stirs aerobic fementation 12h;Based on the weight of step (3) product;
(5) sucrose solution of 5%, inoculating lactic acid bacterium 0.025g/L (5% sucrose solution activation are supplemented
40min), temperature 37 DEG C, aerobic fementation 24h produces acid and produces perfume;Based on step (3) product;
(6) 10% acetic acid bacteria seed liquor is inoculated, 32 DEG C, aerobic fementation 10h, consume ethanol;
(7) fermented product after fermentation, the quick-freezing of-40 DEG C, enters back into vacuum freezing drying cabin and carries out
Lyophilization, finally gives the moisture Golden jujube ferment less than 5%.
Embodiment 2
Allocate after step (3) enzymolysis, add white sugar and Mel so that mixed liquor solubility
Solid content is 23%.
Embodiment 3
Allocate after step (3) enzymolysis, add white sugar and Mel so that mixed liquor solubility
Solid content is 20%.
Above-described is only the preferred embodiments of the present invention.It should be pointed out that, for this area
Those of ordinary skill for, without departing from the principles of the present invention, it is also possible to make some changing
Entering and modification, this is also considered as protection scope of the present invention.
Claims (1)
1. the method preparing Golden jujube ferment, it is characterised in that: it as follows:
(1) Golden jujube dry fruit is cleaned, rehydration, after making beating peeling enucleation, be performing centrifugal separation on obtaining
Fructus Jujubae juice;
(2) complex enzyme hydrolysis: pH is 5.5, and temperature is 55 DEG C, and enzymolysis time is 180min;With step
Suddenly (1) adds the weight meter of Golden jujube dry fruit, and xylanase, the enzyme concentration of glucanase are respectively
500U/g, the enzyme concentration of Novi's letter enzyme and cellulase is respectively 800U/g and 400U/g;
(3) allocate after enzymolysis, add white sugar and Mel so that mixed liquor soluble solid
Content is 17%-23%;Based on step (2) products weight, add yeast extract 0.2%, ammonium sulfate 1.9%,
Zinc chloride 0.1%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.6%, high temperature sterilize 15min at 121 DEG C;
(4) 31 DEG C of entrance fermentation tanks it are cooled to;Based on the weight of step (3) product, inoculation yeast
Bacterium, inoculum concentration: 1%, in fermentation tank, it is passed through filtrated air, stirs aerobic fementation 12h;
(5) sucrose solution of 5%, based on step (3) bulk product, inoculating lactic acid bacterium 0.025g/L are supplemented;
5% sucrose solution activation 40min, temperature 37 DEG C, aerobic fementation 24h produces acid and produces perfume;
(6) inoculate 10% acetic acid bacteria seed liquor, temperature 32 DEG C, aerobic fementation 10h, consume ethanol;
(7) fermented product after fermentation, the quick-freezing of-40 DEG C, enters back into vacuum freezing drying cabin and carries out
Lyophilization, finally gives the moisture Golden jujube ferment less than 5%.
Priority Applications (1)
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CN201610222350.1A CN105831745A (en) | 2016-04-05 | 2016-04-05 | A method of preparing golden silk jujube ferment |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107048361A (en) * | 2017-04-12 | 2017-08-18 | 塔里木大学 | A kind of big rue granatum composite enzyme of jujube and preparation method thereof |
CN107811287A (en) * | 2016-09-13 | 2018-03-20 | 辛庆忠 | A kind of method for preparing maca ferment |
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CN101904504A (en) * | 2010-08-10 | 2010-12-08 | 厦门金日制药有限公司 | Five-element enzyme liquid and preparation method thereof |
CN102160644A (en) * | 2010-12-31 | 2011-08-24 | 山东鼎力枣业食品集团有限公司 | Method for preparing gold thread jujube enzyme from jujube paste of gold thread jujube |
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CN104382053A (en) * | 2014-11-07 | 2015-03-04 | 山东鼎力枣业食品集团有限公司 | Method for producing ziziphus jujuba multienzyme compound probiotics from ziziphus jujuba residues |
CN104872674A (en) * | 2015-06-11 | 2015-09-02 | 宁夏金尚琦生物科技有限公司 | Medlar and red date enzyme and preparation method thereof |
KR101580963B1 (en) * | 2013-07-03 | 2015-12-30 | 곽길우 | Nutrition edible Paste and the manufacture Method |
-
2016
- 2016-04-05 CN CN201610222350.1A patent/CN105831745A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101904504A (en) * | 2010-08-10 | 2010-12-08 | 厦门金日制药有限公司 | Five-element enzyme liquid and preparation method thereof |
CN102160644A (en) * | 2010-12-31 | 2011-08-24 | 山东鼎力枣业食品集团有限公司 | Method for preparing gold thread jujube enzyme from jujube paste of gold thread jujube |
US20140234488A1 (en) * | 2013-02-15 | 2014-08-21 | Alice Chang | Beverage system, including bubble beverage, instant beverage, beverage with dissolved gas, and beverage with ingredient |
KR101580963B1 (en) * | 2013-07-03 | 2015-12-30 | 곽길우 | Nutrition edible Paste and the manufacture Method |
CN104382053A (en) * | 2014-11-07 | 2015-03-04 | 山东鼎力枣业食品集团有限公司 | Method for producing ziziphus jujuba multienzyme compound probiotics from ziziphus jujuba residues |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107811287A (en) * | 2016-09-13 | 2018-03-20 | 辛庆忠 | A kind of method for preparing maca ferment |
CN107048361A (en) * | 2017-04-12 | 2017-08-18 | 塔里木大学 | A kind of big rue granatum composite enzyme of jujube and preparation method thereof |
CN107048361B (en) * | 2017-04-12 | 2020-09-22 | 塔里木大学 | Red date and herba cistanches pomegranate rind composite enzyme and preparation method thereof |
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