CN105062765B - The method that continuous list sterile fermentation prepares cordyceps sinensis rice wine - Google Patents
The method that continuous list sterile fermentation prepares cordyceps sinensis rice wine Download PDFInfo
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Abstract
The present invention relates to a kind of method that continuous single sterile fermentation prepares cordyceps sinensis rice wine, meter Liu Shui cleanings are drained, plus distilled water, are stirred evenly, tinning sealing, sterilizing；It is cooled to 20~25 DEG C, it is standby；The Cordyceps militaris microparticle strain that bacterial strain deposit number is NKHB CM209635 is cultivated, Cordyceps militaris microparticle liquid fungus seed is accessed in rice tank, lucifuge 5~10d of culture, fermentation is shown in 3~7d of optical culture, sterilized, plus sterilized water；Access plus sterile water-reducible Rhizopus oryzae liquid microparticle strain, cultivate 36~48h；Access again plus sterile water-reducible saccharomyces cerevisiae strain, cultivate 3~7d, total acid, total reducing sugar, alcoholic strength are detected every 12h；Packing, packaging；60Co γ irradiation bomb normal temperature irradiation sterilizations.The present invention is continuously fermented using Cordyceps militaris spawn, Rhizopus oryzae strain, saccharomyces cerevisiae strain under the conditions of single-minded, and cost is low, gained cordyceps sinensis rice wine inclusion active component, nutritious.
Description
Technical field
The present invention relates to a kind of method that continuous single sterile fermentation prepares cordyceps sinensis rice wine.
Background technology
Cordyceps militaris (Cordyceps militaris), belongs to Ascomycotina, ergot Zoopagales, Clavicipitaceae, the mould of Cordyceps
Formula kind.Cordyceps militaris contains various active material, including Cordyceps sinensis polysaccharide, cordycepin, cordycepic acid, adenosine, nucleosides, ergosterol, SOD
Deng.
Rice wine is also known as fermented glutinour rice, sweet rice wine, is a kind of China's traditional fermented food among the people, and existing the edible of two thousand yearses is gone through
History, alcohol content is low, taste is fragrant and sweet pure and sweet, deep to be liked by the numerous common people.Rice wine is sent out using glutinous rice as raw material with natural distiller's yeast is made by oneself
Ferment is made, containing nutriments such as abundant reduced sugar, amino acid, microorganisms, low alcohol, have clearing and activating the channels and collaterals, blood-nourishing tonifying Qi,
The effect of nourishing Yin and moistening lung.Conventional processing methods make rice wine process simply, using glutinous rice, rice as raw material, use sweet wine koji fermentation.Sweet tea
Distiller's yeast belongs to mixed starters, containing a large amount of saccharomycete, outside a small amount of rhizopus, also a large amount of miscellaneous bacterias, so, distiller's yeast quality is very unstable
It is fixed, the rice wine thick taste worked it out and sweet taste is not enough, or alcoholic strength is very low, or even makes rice wine failure, it is difficult to accomplish to produce
Rice wine mouthfeel remain the same from beginning to end.
A kind of preparation side for worm grass rice wine for soothing nerves and replenishing brain that existing rice wine and preparation method, such as CN102477394 are announced
Method, offers sacriffices to the gods or the spirits of the dead 30 grams using 10 grams of cordyceps sinensis, 30 grams of dried longan pulp, 20 grams of ginseng, 30 grams of jujube, structure, is extracted by rice wine.CN
A kind of 103382427 health-care rice wines announced, its brewage process is secondary fermentation, first millet, koji for uncooked material and underground deep layer
Fermented after water mixing, it is fermented after carry out distilling out white wine again, then using white wine as wine base, millet rice increases jujube and soaks laggard
Row boiling, increases Qu Jinhang fermentations, is squeezed after the completion of fermentation again after boiling, rock sugar, honey and Chinese medicine are added after squeezing auxiliary
Toning is blent in material progress, is then killed virus after filtering, through filling and sealing after the assay was approved, is finally prepared to finished rice wine and is packaged into
Storehouse.
The content of the invention
The purpose of the present invention is to be directed to the above-mentioned state of the art, it is desirable to provide a kind of active component, nutritious；Preparation method
Pollution-free, noresidue, the method that operation is continuous, cost is low, efficiency high continuous single sterile fermentation prepares cordyceps sinensis rice wine.
The implementation of the object of the invention is that the method that continuous list sterile fermentation prepares cordyceps sinensis rice wine is comprised the following steps that：
(1) rice is screened, flowing water 40~90s of stirring and washing is drained；
(2) in 1-1.5kg meters plus distilled water, the ratio of water and rice is 1.2~3:1, stir evenly；
(3) by rice tinning, sealing, in 121 DEG C, 1.034 × 105Pa, sterilize 15~30min；20~25 DEG C are cooled to,
It is standby；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHB CM209635,
Cordyceps militaris spawn culture medium：100~200g of ground rice, 20~40g of sucrose, dusty yeast 6~10g, MgSO4/MgCl2 3
~6, KH2PO4/K2HPO43~4g, plus sterile distilled water is to 1000ml；In 20~23 DEG C of temperature, pH 6~7, shearing rotating speed
5~6d is cultivated under the conditions of 800~1200rpm, the Cordyceps militaris microparticle strain that bacterial strain deposit number is NKHB CM209635 is obtained,
It is standby；
(5) glutinous rice quality 1~10%, the Cordyceps militaris microparticle liquid of step (4) culture are accessed in the glutinous rice tank of step (3)
Body strain；20~25 DEG C of fermentations, lucifuge 5~10d of culture, every 12h stirrings once；
(6) 20~25 DEG C of fermentations, are shown in 3~7d of optical culture, and light intensity is 100~1200Lux, every 12h stirrings once；
(7) fermentation, 115 DEG C, 0.676 × 10 are stopped4Pa, sterilize 15min；Plus the sterilized water of its quality 10~30%；
(8) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCC AF93150,
Rhizopus oryzae bacterium culture medium：100~200g of ground rice, 20~60g of potato leaching powder, sucrose 20~40g, MgSO4/
MgCl20.5~2g, KH2PO4/K2HPO41~4g, adds water to 1000ml；In 28 DEG C of temperature, pH 6~7, shearing rotating speed 800~
1200rpm, cultivates 36~48h, obtains the Rhizopus oryzae liquid microparticle strain that bacterial strain deposit number is CCTCC AF93150, standby；
Wherein KH2PO4/K2HPO4=1:1；
(9) 1~10%% of screening meter Zhi Liang in step (1), the Rhizopus oryzae liquid microparticle bacterium of step (8) culture are taken
Plant, plus sterilized water is diluted in 3~5 times, the tank of access step (8), 28 DEG C of 36~48h of culture；
(10) culture bacterial strain deposit number is CCTCC AY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：100~200g of ground rice, 3~8g of casein peptone, 3~10g of fructus hordei germinatus leaching powder, sucrose 10
~40g, 3~6g of yeast extract, adds water to 1000ml；Condition of culture：28 DEG C of temperature, pH 6~7, shearing rotating speed 800~
1200rpm, cultivates 32~48h, obtains bacterial strain deposit number for CCTCC AY93158 saccharomyces cerevisiae strains, standby；
(11) 1~5% of screening meter Zhi Liang in step (1), the saccharomyces cerevisiae strain of step (10) culture, plus sterilized water are taken
It is diluted in 3~5 times, the tank of access step (9), 28 DEG C of 3~7d of culture detect total acid, total reducing sugar, alcoholic strength every 12h；
(12) stop fermentation, dispense, packaging；
(13)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 1~10kGy.
Cordyceps are quoted in traditional food, the means converted by biofermentation introduced in rice wine food nutrition and
Active material, makes the innovation upgrading of rice wine food.
The present invention using Cordyceps militaris spawn, Rhizopus oryzae strain, saccharomyces cerevisiae strain it is each it is single-minded under the conditions of continuously ferment,
Three kinds of simple bacterial strains continuously ferment to convert introduces physiological activator, such as cordycepin, Cordyceps sinensis polysaccharide, adenosine in rice wine,
Cost is low, gained cordyceps sinensis rice wine inclusion active component, nutritious；Preparation method is pollution-free, noresidue, green and healthy, operation
Continuously, cost is low, efficiency high.
Brief description of the drawings
Fig. 1 is Cordyceps militaris spawn figure,
Fig. 2 is cordyceps sinensis rice wine, common glutinous rice wine, general brown rice sweet rice wine figure,
Fig. 3 is adenosine reference substance HPLC chromatogram,
Fig. 4 is cordyceps sinensis rice wine adenosine HPLC chromatogram.
Embodiment
Using the present invention, rice is screened, flowing water cleaning is drained, plus distilled water, the ratio of water and rice is 1~3:1, stir evenly, fill
Tank is sealed, sterilizing.It is preferred that, in step (2), the ratio of water and rice is:1.5~2:1.Screened rice is glutinous rice, brown rice or broken
Rice.
It is cooled to 20~25 DEG C, it is standby；Cultivate the Cordyceps militaris microparticle bacterium that bacterial strain deposit number is NKHB CM209635
Kind.Cordyceps militaris spawn culture medium：100~200g of ground rice, 20~40g of sucrose, dusty yeast 6~10g, MgSO4/MgCl23~6g,
KH2PO4/K2HPO43~4g, plus sterile distilled water is to 1000ml；In 20~23 DEG C of temperature, pH 6~7, shearing rotating speed 800~
5~6d is cultivated under the conditions of 1200rpm, the Cordyceps militaris microparticle bacterium that the bacterial strain deposit number shown in Fig. 1 is NKHB CM209635 is obtained
Kind.Thalli granule particle diameter is 0.2~0.7mm, and cell concentration is 103~105Individual/ml.
It is preferred that, Cordyceps militaris spawn culture medium：150~200g of ground rice, 20~30g of sucrose, dusty yeast 6~8g, MgSO4/
MgCl25g, KH2PO4/K2HPO44g, plus sterile distilled water is to 1000ml；In 22 DEG C of temperature, pH 7, shearing rotating speed 1000rpm
Under the conditions of cultivate 6d.Cordyceps militaris thalline grain diameter is 0.3~0.5mm, and cell concentration is 5 × 103~104Individual/ml.
In step (5), (6), 1~10% Cordyceps militaris microparticle liquid fungus seed of access access, lucifuge in glutinous rice tank
5~10d is cultivated, 3~7d of optical culture is shown in fermentation, and light intensity is 100~1200Lux.It is preferred that, in glutinous rice tank access access 2~
3~5d of optical culture is shown in 3% Cordyceps militaris microparticle liquid fungus seed, lucifuge 5~7d of culture, fermentation, light intensity is 200~
500Lux.Sterilizing, plus sterilized water.
Cultivate the Rhizopus oryzae strain that bacterial strain deposit number is CCTCC AF93150, Rhizopus oryzae bacterium culture medium：Ground rice 100
~200g, 20~60g of potato leaching powder, sucrose 20~40g, MgSO4/MgCl20.5~2g, KH2PO4/K2HPO41~4g, plus
Water is to 1000ml；In 28 DEG C of temperature, pH 6~7,800~1200rpm of shearing rotating speed cultivate 36~48h.It is preferred that, step (8)
In, Rhizopus oryzae bacterium culture medium：140~160g of ground rice, 30~40g of potato leaching powder, sucrose 20~30g, MgSO4/MgCl2
0.5~1g, KH2PO4/K2HPO42~3g, adds water to 1000ml；In 28 DEG C of temperature, pH 6.5, shearing rotating speed 1000rpm, training
Support 40h.
The Rhizopus oryzae strain grain diameter that step (8) obtained strains deposit number is CCTCC AF93150 is 0.2~
0.5mm, cell concentration is 103~105Individual/ml.
1~10% Rhizopus oryzae microparticle liquid spawn of screening meter Zhi Liang in step (1), plus sterilized water is taken to be diluted to 3~5
Times, in the tank of access step (8), 28 DEG C of 36~48h of culture.It is preferred that, 3 of screening meter Zhi Liang in step (1) are taken in step (9)
~5% Rhizopus oryzae microparticle liquid spawn, plus sterilized water are diluted to 5 times, in the tank of access step (8), and 28 DEG C of cultures 36~
40h。
Rhizopus oryzae liquid spawn inoculum concentration is 3~5%, plus sterilized water dilutes 5 times, and fermentation time is 36~40h.
It is CCTCC AY93158 saccharomyces cerevisiae strains, saccharomyces cerevisiae strain culture medium to cultivate bacterial strain deposit number：Ground rice
100~200g, 3~8g of casein peptone, 3~10g of fructus hordei germinatus leaching powder, 10~40g of sucrose, 3~6g of yeast extract, are added water to
1000ml；Condition of culture：28 DEG C of temperature, pH 6~7,800~1200rpm of shearing rotating speed cultivate 32~48h.It is preferred that, step
(10) in, saccharomyces cerevisiae strain culture medium：150~200g of ground rice, 4~6g of casein peptone, 3~5g of fructus hordei germinatus leaching powder, sucrose 10~
20g, 4~5g of yeast extract, adds water to 1000ml；Condition of culture：28 DEG C of temperature, pH 6.5, shearing rotating speed 800rpm, culture
32h.The saccharomyces cerevisiae strain concentration of step (10) culture is 5 × 106~107Individual/ml.
Ground rice used is brown rice or powder of cracking rice in step (4), (8), (10).
Take 1~5% saccharomyces cerevisiae strain of screening meter Zhi Liang in step (1), it is preferred that take screening rice in step (1)
The saccharomyces cerevisiae strain of the 2~3% of quality.3~7d is cultivated, total acid, total reducing sugar, alcoholic strength are detected every 12h.Packing, packaging；60Co- γ irradiation bomb normal temperature irradiation sterilizations obtain cordyceps sinensis rice wine.Careless cellulose content 0.3~1.0 ‰ of gained cordyceps sinensis rice wine worm, cordyceps sinensis is more
Sugared content 0.2~3.0%, adenosine content 0.5~2.0 ‰, alcohol content 1~10%, 1.0~10g/L of total acid content, total reducing sugar contains
Measure 100g~200g/L, content of reducing sugar 5~10%, solid content 20~30%.
Using60Co- γ irradiation bomb normal temperature irradiation sterilizations, nutrition, active component are not destroyed, outer packing is not destroyed.
Common glutinous rice wine, general brown rice sweet wine are shown in Fig. 2 with the rice wine cordyceps sinensis rice wine figure prepared using the present invention, from Fig. 1,2
It can be seen that, cordyceps sinensis rice wine is demonstrated by the color and luster and form of cordyceps sinensis in appearance, illustrates that cordyceps sinensis rice wine has the attribute of cordyceps sinensis.
Adenosine reference substance HPLC chromatogram is shown in Fig. 3, and cordyceps sinensis rice wine adenosine HPLC chromatogram is shown in Fig. 4, from figure, 3,4 visible cordyceps sinensis
Rice wine and adenosine standard items have the purpose peak of identical retention time, illustrate in cordyceps sinensis rice wine containing adenosine species activity into
Point.
The present invention is described in detail with specific embodiment below：
Embodiment 1
(1) screening glutinous rice, flowing water 40~60s of stirring and washing, drainage is to stop without turbidity, colourity, is drained；
(2) add 1.5L distilled water in 1kg glutinous rice, stir evenly；
(3) by glutinous rice tinning, sealing, in 121 DEG C, 1.034 × 105Pa, sterilize 15min；It is cooled to 20~25 DEG C, it is standby
With；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHB CM209635,
Cordyceps militaris spawn culture medium：Coarse rice powder 150g, sucrose 30g, dusty yeast 6g, MgSO4/MgCl23g, KH2PO4/
K2HPO44g, plus sterile distilled water is to 1000ml；In 22 DEG C of temperature, pH6.5 cultivates 6d under the conditions of shearing rotating speed 1000rpm, obtained
Bacterial strain deposit number is NKHB CM209635 Cordyceps militaris microparticle strain, standby；Wherein KH2PO4/K2HPO4=1:1；
(5) glutinous rice quality 5%, the Cordyceps militaris microparticle liquid bacteria of step (4) culture are accessed in the glutinous rice tank of step (3)
Kind；20 DEG C of fermentations, lucifuge culture 7d, every 12h stirrings once；
(6) 20 DEG C of fermentations, are shown in optical culture 5d, light intensity is 1000Lux, every 12h stirrings once；
(7) fermentation, 115 DEG C, 0.676 × 10 are stopped4Pa, sterilize 15min；Plus the sterilized water of its quality 10%；
(8) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCC AF93150,
Rhizopus oryzae bacterium culture medium：Coarse rice powder 100g, potato leaching powder 50g, sucrose 20g, MgSO4/MgCl20.5g,
KH2PO4/K2HPO41g, adds water to 1000ml；In 28 DEG C of temperature, pH 6.5, shearing rotating speed 1000rpm cultivate 40h, obtain bacterial strain
Deposit number is CCTCC AF93150 Rhizopus oryzae strain, standby；
Wherein KH2PO4/K2HPO4=1:1；
(9) 5% of screening meter Zhi Liang in step (1), the Rhizopus oryzae liquid microparticle strain of step (8) culture, plus nothing are taken
Bacterium water is diluted in 5 times, the tank of access step (8), 28 DEG C of culture 40h；
(10) culture bacterial strain deposit number is CCTCC AY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：Coarse rice powder 100g, casein peptone 5g, fructus hordei germinatus leaching powder 3g, sucrose 10g, yeast extract
3g, adds water to 1000ml；Condition of culture：28 DEG C of temperature, pH 6.5, shearing rotating speed 1000rpm cultivate 40h, obtain bacterial strain preservation volume
Number be CCTCC AY93158 saccharomyces cerevisiae strains, it is standby；
(11) 5% of screening meter Zhi Liang in step (1) is taken, the saccharomyces cerevisiae strain of step (10) culture, plus sterilized water are dilute
Release to 3 times, in the tank of access step (9), 28 DEG C of culture 5d detect total acid, total reducing sugar, alcoholic strength every 12h；
(12) stop fermentation, dispense, packaging；
(13)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 3kGy.
Embodiment 2, be the same as Example 1, unlike,
(2) add 1.2L distilled water in 1kg brown rice, stir evenly；
(3) by brown rice tinning, sealing, in 121 DEG C, 1.034 × 105Pa, sterilize 25min；It is cooled to 20~25 DEG C, it is standby
With；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHB CM209635,
Cordyceps militaris spawn culture medium：Coarse rice powder 100g, sucrose 40g, dusty yeast 8g, MgSO4/MgCl25g, KH2PO4/
K2HPO44g, plus sterile distilled water is to 1000ml；In 20 DEG C of temperature, pH6.5 cultivates 6d under the conditions of shearing rotating speed 1200rpm, standby
With；
(5) the Cordyceps militaris microparticle liquid spawn of brown rice quality 3% is accessed in brown rice tank；20 DEG C of fermentations, lucifuge culture
5d；
(6) 22 DEG C of fermentations, are shown in optical culture 5d, light intensity is 500Lux, every 12h stirrings once；
(7) stop fermentation, plus its quality 30% sterilized water；
(8) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCC AF93150,
Rhizopus oryzae bacterium culture medium：Coarse rice powder 150g, potato leaching powder 30g, sucrose 30g, MgSO4/MgCl21g,
KH2PO4/K2HPO42g, adds water to 1000ml；In 28 DEG C of temperature, pH 6.0, shearing rotating speed 1000rpm cultivate 48h；
(9) the 3% Rhizopus oryzae liquid microparticle strain of screening meter Zhi Liang in step (1), plus sterilized water is taken to be diluted to 3
Times, in the tank of access step (8), 28 DEG C of culture 48h；
(10) culture bacterial strain deposit number is CCTCC AY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：Coarse rice powder 200g, casein peptone 6g, fructus hordei germinatus leaching powder 7g, sucrose 20g, yeast extract
5g, adds water to 1000ml；PH 7, shearing rotating speed 1000rpm, cultivate 36h；
(11) the 3% saccharomyces cerevisiae strain of screening meter Zhi Liang in step (1), plus 3 times of sterilized water dilution culture are taken；
(13)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 10kGy.
Embodiment 3, be the same as Example 1, unlike,
(1) screening is cracked rice, and flowing water rinses 50~70s, drains；
(2) add 3L distilled water during 1kg cracks rice, stir evenly；
(3) will crack rice tinning, sealing, in 121 DEG C, 1.034 × 105Pa, sterilize 20min；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHB CM209635,
Cordyceps militaris spawn culture medium：Crack rice powder 200g, sucrose 20g, dusty yeast 10g, MgSO4/MgCl26g, KH2PO4/
K2HPO43g, plus sterile distilled water is to 1000ml；In 23 DEG C of temperature, pH6.0 cultivates 6d under the conditions of shearing rotating speed 1000rpm；
(5) the Cordyceps militaris microparticle liquid spawn for quality 10% of cracking rice is accessed in tank of cracking rice；22 DEG C of fermentations, lucifuge culture
10d；
(6) 22 DEG C of fermentations, are shown in optical culture 3d, light intensity is 200Lux；
(7) stop fermentation, plus its quality 20% sterilized water；
(8) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCC AF93150,
Rhizopus oryzae bacterium culture medium：Crack rice powder 100g, potato leaching powder 60g, sucrose 20g, MgSO4/MgCl22g,
KH2PO4/K2HPO44g, adds water to 1000ml；In 28 DEG C of temperature, pH 7.0, shearing rotating speed 800rpm cultivate 36h；
(9) the 1% Rhizopus oryzae liquid microparticle strain of screening meter Zhi Liang in step (1), plus sterilized water is taken to be diluted to 3
Times, in the tank of access step (8), 28 DEG C of culture 36h；
(10) culture bacterial strain deposit number is CCTCC AY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：Crack rice powder 150g, casein peptone 3g, fructus hordei germinatus leaching powder 10g, sucrose 40g, yeast extract
6g, adds water to 1000ml；PH 6.0, shearing rotating speed 1000rpm, cultivate 48h；
(11) the 5% saccharomyces cerevisiae strain of screening meter Zhi Liang in step (1), plus 5 times, 28 DEG C of sterilized water dilution culture are taken
Cultivate 3d；
(13)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 1kGy.
Embodiment 4, be the same as Example 1, unlike,
(1) glutinous rice is screened, flowing water rinses 50~60s, drained；
(2) add 2L distilled water in 1.5kg glutinous rice, stir evenly；
(3) by glutinous rice tinning, sealing, in 121 DEG C, 1.034 × 105Pa, sterilize 20min；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHB CM209635,
Cordyceps militaris spawn culture medium：Coarse rice powder 120g, sucrose 40g, dusty yeast 7g, MgSO4/MgCl24g, KH2PO4/
K2HPO44g, plus sterile distilled water is to 1000ml；In 23 DEG C of temperature, pH6.0 cultivates 6d under the conditions of shearing rotating speed 1200rpm；
(5) the Cordyceps militaris microparticle liquid spawn of glutinous rice quality 1% is accessed in glutinous rice tank；25 DEG C of fermentations, lucifuge culture
10d；
(6) 25 DEG C of fermentations, are shown in optical culture 6d, light intensity is 900Lux；
(7) stop fermentation, plus its quality 15% sterilized water；
(8) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCC AF93150,
Rhizopus oryzae bacterium culture medium：Coarse rice powder 100g, potato leaching powder 20g, sucrose 40g, MgSO4/MgCl20.5g,
KH2PO4/K2HPO43g, adds water to 1000ml；In 28 DEG C of temperature, pH6.5, shearing rotating speed 800rpm cultivate 36h；
(9) the 10% Rhizopus oryzae liquid microparticle strain of screening meter Zhi Liang in step (1), plus sterilized water is taken to be diluted to 3
Times, in the tank of access step (8), 28 DEG C of culture 42h；
(10) culture bacterial strain deposit number is CCTCC AY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：Coarse rice powder 200g, casein peptone 8g, fructus hordei germinatus leaching powder 3g, sucrose 10g, yeast extract
3g, adds water to 1000ml；In 28 DEG C of temperature, pH 6.5, shearing rotating speed 1200rpm cultivate 32h；
(11) the 2% saccharomyces cerevisiae strain of screening meter Zhi Liang in step (1), plus 5 times, 28 DEG C of sterilized water dilution culture are taken
Cultivate 4d；
(13)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 5kGy.
Embodiment 5, be the same as Example 1, unlike,
(1) brown rice is screened, flowing water rinses 60~90s, drained；
(2) add 2.5L distilled water in 1.5kg brown rice, stir evenly；
(3) by brown rice tinning, sealing, in 121 DEG C, 1.034 × 105Pa, sterilize 25min；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHB CM209635,
Cordyceps militaris spawn culture medium：Coarse rice powder 160g, sucrose 20g, dusty yeast 8g, MgSO4/MgCl25g, KH2PO4/
K2HPO44g, plus sterile distilled water is to 1000ml；In 22 DEG C of temperature, pH6.0 cultivates 5d under the conditions of shearing rotating speed 1200rpm；
(5) the Cordyceps militaris microparticle liquid spawn of glutinous rice quality 2% is accessed in brown rice tank；25 DEG C of fermentations, lucifuge culture
10d；
(6) 25 DEG C of fermentations, are shown in optical culture 7d, light intensity is 100Lux；
(7) stop fermentation, plus its quality 20% sterilized water；
(8) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCC AF93150,
Rhizopus oryzae bacterium culture medium：Coarse rice powder 200g, potato leaching powder 40g, sucrose 40g, MgSO4/MgCl21g,
KH2PO4/K2HPO44g, adds water to 1000ml；In 28 DEG C of temperature, pH 7.0, shearing rotating speed 1200rpm cultivate 46h；
(9) the 7% Rhizopus oryzae liquid microparticle strain of screening meter Zhi Liang in step (1), plus sterilized water is taken to be diluted to 5
Times, in the brown rice tank of access step (8), 28 DEG C of culture 46h；
(10) culture bacterial strain deposit number is CCTCC AY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：Coarse rice powder 160g, casein peptone 8g, fructus hordei germinatus leaching powder 1g, sucrose 10g, yeast extract
3g, adds water to 1000ml；28 DEG C of temperature, pH 6.5, shearing rotating speed 900rpm cultivate 42h；
(11) the 4% saccharomyces cerevisiae strain of screening meter Zhi Liang in step (1), plus 3 times, 28 DEG C of sterilized water dilution culture are taken
Cultivate 7d；
(13)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 8kGy.
Embodiment 6, be the same as Example 1, unlike,
(1) screening is cracked rice, and flowing water rinses 60~90s, drains；
(2) add 2L distilled water during 1.5kg cracks rice, stir evenly；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHB CM209635,
Cordyceps militaris spawn culture medium：Crack rice powder 150g, sucrose 30g, dusty yeast 10g, MgSO4/MgCl26g, KH2PO4/
K2HPO44g, plus sterile distilled water is to 1000ml；In 20 DEG C of temperature, pH7.0 cultivates 5d under the conditions of shearing rotating speed 1000rpm；
(5) the Cordyceps militaris microparticle liquid spawn for quality 8% of cracking rice is accessed in glutinous rice tank；25 DEG C of fermentations, lucifuge culture
5d；
(6) 21 DEG C of fermentations, are shown in optical culture 3d, light intensity is 1200Lux；
(7) stop fermentation, plus its quality 30% sterilized water；
(8) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCC AF93150,
Rhizopus oryzae bacterium culture medium：Crack rice powder 150g, potato leaching powder 30g, sucrose 20g, MgSO4/MgCl20.5g,
KH2PO4/K2HPO43g, adds water to 1000ml；In 28 DEG C of temperature, pH 7.0, shearing turns 1000rpm, cultivates 40h；
(9) the 5% Rhizopus oryzae liquid microparticle strain of screening meter Zhi Liang in step (1), plus sterilized water is taken to be diluted to 3
Times, in the tank of cracking rice of access step (8), 28 DEG C of culture 46h；
(10) culture bacterial strain deposit number is CCTCC AY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：Crack rice powder 200g, casein peptone 3g, fructus hordei germinatus leaching powder 5g, sucrose 30g, yeast extract
6g, adds water to 1000ml；28 DEG C of temperature, pH 6.0, shearing rotating speed 800rpm cultivate 32h；
(11) the 5% saccharomyces cerevisiae strain of screening meter Zhi Liang in step (1), plus 3 times of sterilized water dilution culture, access are taken
Step (9) is cracked rice in tank, 28 DEG C of culture 6d；
(13)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 6kGy.
Claims (13)
1. the method for preparing cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Comprise the following steps that：
(1) rice is screened, flowing water 40~90s of stirring and washing is drained；
(2) in 1-1.5kg meters plus distilled water, the ratio of water and rice is 1.2~3:1, stir evenly；
(3) by rice tinning, sealing, in 121 DEG C, 1.034 × 105Pa, sterilize 15~30min；It is cooled to 20~25 DEG C, it is standby；
(4) the Cordyceps militaris microparticle strain that culture bacterial strain deposit number is NKHBCM209635,
Cordyceps militaris spawn culture medium：100~200g of ground rice, 20~40g of sucrose, dusty yeast 6~10g, MgSO4/MgCl23~6g,
KH2PO4/K2HPO43~4g, plus sterile distilled water is to 1000ml；In 20~23 DEG C of temperature, pH6~7, shearing rotating speed 800~
5~6d is cultivated under the conditions of 1200rpm, Cordyceps militaris microparticle liquid spawn is obtained, it is standby；
(5) meter Zhi Liang 1~10% Cordyceps militaris microparticle liquid spawn is accessed in the rice tank of step (3)；First 20~
25 DEG C of fermentations, lucifuge 5~10d of culture, every 12h stirrings once；Then in 20~25 DEG C of fermentations, 3~7d of optical culture, light intensity are seen
For 100~1200Lux, stirred once every 12h；Stop fermentation, in 115 DEG C, 0.676 × 104Sterilize 15min under the conditions of Pa；
Plus the sterilized water of its quality 10~30%；
(6) the Rhizopus oryzae strain that culture bacterial strain deposit number is CCTCCAF93150,
Rhizopus oryzae bacterium culture medium：100~200g of ground rice, 20~60g of potato leaching powder, sucrose 20~40g, MgSO4/
MgCl20.5~2g, KH2PO4/K2HPO41~4g, adds water to 1000ml；In 28 DEG C of temperature, pH6~7, shearing rotating speed 800~
1200rpm, cultivates 36~48h, obtains Rhizopus oryzae microparticle liquid spawn, standby；
Wherein KH2PO4/K2HPO4=1:1；
(7) the Rhizopus oryzae microparticle liquid spawn of screening meter Zhi Liang 1~10% in step (1), plus sterilized water is taken to be diluted to 3
~5 times, access step (5) tank in, 28 DEG C culture 36~48h；
(8) culture bacterial strain deposit number is CCTCCAY93158 saccharomyces cerevisiae strains,
Saccharomyces cerevisiae strain culture medium：100~200g of ground rice, 3~8g of casein peptone, 3~10g of fructus hordei germinatus leaching powder, sucrose 10~
40g, 3~6g of yeast extract, adds water to 1000ml；Condition of culture：28 DEG C of temperature, pH6~7,800~1200rpm of shearing rotating speed,
32~48h is cultivated, saccharomyces cerevisiae liquid spawn is obtained, it is standby；
(9) 1~5% saccharomyces cerevisiae liquid spawn of screening meter Zhi Liang in step (1), plus sterilized water is taken to be diluted to 3~5
Times, in the tank of access step (7), 28 DEG C of 3~7d of culture detect total acid, total reducing sugar, alcoholic strength every 12h；Stop fermentation, obtain worm
Careless rice wine, is dispensed, packaging；
(10)60Co- γ irradiation bomb normal temperature irradiation sterilizations, radiation absorber amount is 1~10kGy.
2. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Step (1) is screened
Rice is glutinous rice, brown rice or cracks rice, and the ratio of step (2) water and rice is:1.5~2:1.
3. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Pupa worm in step (4)
Careless bacterium culture medium：150~200g of ground rice, 20~30g of sucrose, dusty yeast 6~8g, MgSO4/MgCl25g, KH2PO4/
K2HPO44g, plus sterile distilled water is to 1000ml；In 22 DEG C of temperature, pH7 cultivates 6d under the conditions of shearing rotating speed 1000rpm.
4. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Step (4), (6),
(8) ground rice used is coarse rice powder or powder of cracking rice in.
5. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Bacterium obtained by step (4)
Strain deposit number be NKHBCM209635 Cordyceps militaris microparticle liquid spawn, Cordyceps militaris thalline grain diameter be 0.2~
0.7mm, cell concentration is 103~105Individual/ml.
6. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Bacterium obtained by step (4)
Strain deposit number be NKHBCM209635 Cordyceps militaris microparticle liquid spawn, Cordyceps militaris thalline grain diameter be 0.3~
0.5mm, cell concentration is 5 × 103~104Individual/ml.
7. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Step (5), in,
Rice tank in access access 2~3% Cordyceps militaris microparticle liquid fungus seeds, lucifuge 5~7d of culture, fermentation, see optical culture 3~
5d, light intensity is 200~500Lux.
8. the method for preparing cordyceps sinensis rice wine of continuously fermenting according to claim 1 or 7, it is characterised in that：In step (6),
Rhizopus oryzae bacterium culture medium：100~150g of ground rice, 30~40g of potato leaching powder, sucrose 20~30g, MgSO4/MgCl20.5~
1g, KH2PO4/K2HPO42~3g, adds water to 1000ml；In 28 DEG C of temperature, pH6.5, shearing rotating speed 1000rpm cultivate 40h.
9. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：Bacterium obtained by step (6)
The Rhizopus oryzae microparticle liquid spawn that strain deposit number is CCTCCAF93150, strain grain diameter is 0.2~0.5mm, thalline
Concentration is 103~105Individual/ml.
10. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：In step (7), 3 are taken
~5% Rhizopus oryzae microparticle liquid spawn, plus sterilized water are diluted to 5 times, in the tank of access step (5), and 28 DEG C of cultures 36~
40h。
11. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：In step (8), make
Brewer yeast bacterium culture medium：150~200g of ground rice, 3~6g of casein peptone, 3~5g of fructus hordei germinatus leaching powder, 10~20g of sucrose, yeast leaching
3~5g of powder, adds water to 1000ml；Condition of culture：28 DEG C of temperature, pH6.5, shearing rotating speed 800rpm cultivate 32h.
12. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：In step (8), training
It is 5 × 10 to support obtained saccharomyces cerevisiae liquid spawn concentration6~107Individual/ml.
13. method according to claim 1 that prepare cordyceps sinensis rice wine of continuously fermenting, it is characterised in that：In step (9), take
Screening meter Zhi Liang 2~3% saccharomyces cerevisiae liquid spawn is fermented in step (1).
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|---|---|---|---|---|
| CN101717709A (en) * | 2009-12-24 | 2010-06-02 | 孙国明 | Preparation method of cordyceps wine |
| CN102329718A (en) * | 2011-10-20 | 2012-01-25 | 安徽农业大学 | Method for preparing vinegar by continuously fermenting multi-strain immobilized cell composition |
| CN102823427A (en) * | 2012-08-30 | 2012-12-19 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for producing cordyceps militaris microparticle liquid strain by rough rices |
| CN103666974A (en) * | 2013-12-19 | 2014-03-26 | 蒙金妹 | Method for breeding sticky rice cordyceps sinensis healthcare wine |
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| CN101717709A (en) * | 2009-12-24 | 2010-06-02 | 孙国明 | Preparation method of cordyceps wine |
| CN102329718A (en) * | 2011-10-20 | 2012-01-25 | 安徽农业大学 | Method for preparing vinegar by continuously fermenting multi-strain immobilized cell composition |
| CN102823427A (en) * | 2012-08-30 | 2012-12-19 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for producing cordyceps militaris microparticle liquid strain by rough rices |
| CN103666974A (en) * | 2013-12-19 | 2014-03-26 | 蒙金妹 | Method for breeding sticky rice cordyceps sinensis healthcare wine |
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