CN104489641B - Cordyceps sinensis health powder effervescent tablet product and preparation method thereof - Google Patents

Cordyceps sinensis health powder effervescent tablet product and preparation method thereof Download PDF

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CN104489641B
CN104489641B CN201410722462.4A CN201410722462A CN104489641B CN 104489641 B CN104489641 B CN 104489641B CN 201410722462 A CN201410722462 A CN 201410722462A CN 104489641 B CN104489641 B CN 104489641B
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powder
enzymolysis
cordycepses
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pleurotus nebrodensis
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CN104489641A (en
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邵素英
李政
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YIWU JINYU INFORMATION TECHNOLOGY CO., LTD.
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Yiwu Jinyu Information Technology Co Ltd
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Abstract

The invention relates to a cordyceps sinensis health powder effervescent tablet product and a preparation method thereof, belongs to the field of edible mushroom deep processing, and relates to an edible mushroom product and a preparation method thereof. The cordyceps sinensis health powder effervescent tablet product is prepared from the following components in parts by weight: 10-20 parts of morchella esculenta enzymolysis powder, 15-20 parts of pleurotus nebrodensis-cordyceps sinensis enzymolysis powder, 2-5 parts of animal proteolysis powder, 2-5 parts of cordyceps sinensis hypha powder, 2-5 parts of hawthorn powder, 20-40 parts of disintegrant, 30-80 parts of diluent, 0.5-2 parts of lubricant and 2-10 parts of corrigent. The product disclosed by the invention has the characteristics of being prominent in mushroom fragrance, proper in taste, and rich in nutrition; the preparation of the animal proteolysis powder is carried out by combining the processes of homogenizing, enzymolysis medium-term high-temperature treatment and sectioned enzymolysis treatment, thus an enzyme is promoted to play a role; the product is delicious in taste and rich in many nutritional function ingredients, and is an edible mushroom deep-processing product integrating nutrition, nourishment and human immunity function regulation.

Description

Chinese caterpillar fungus health powder effervescent tablet products and preparation method thereof
Technical field:
The invention belongs to edible fungi field of deep, particularly to a kind of edible fungus and preparation method thereof.
Background technology:
Pleurotus nebrodensis are a kind of rare edible fungi, and according to St Foods Q Supervision & Testing Center analysis, Pleurotus nebrodensis protein content is high Reach 14.7%.18 kinds of aminoacid are contained, 8 kinds of aminoacid wherein needed by human all contain in protein.Every gram of Pleurotus nebrodensis contain 190 milligrams of polysaccharide.In aminoacid, lysine is 569 milligrams/100 grams, for 2.8 times of general Pleurotus ostreatus;Leucine 790.2 Milligram/100 grams, for 2.6 times of general Pleurotus ostreatus;Isoleucine is 470.1 milligrams/100 grams, for 2.5 times of general Pleurotus ostreatus; 674.6 milligrams/100 grams of L-Valine, for 2.2 times of general Pleurotus ostreatus, other human body essential amino acid contents are also high than general Pleurotus ostreatus 1.9-2.7 times, especially glutamic acid and arginine content are especially high.The content of Pleurotus nebrodensis not only aminoacid is high, surprising It is arginine, lysine, these are conducive to the nutritional factorss of intelligent development, than the Flammulina velutiper (Fr.) Sing content being referred to as intelligence mushroom in the world also Will height.Pleurotus nebrodensis also contain substantial amounts of multivitamin and multi mineral element.Contained by Pleurotus nebrodensis, a large amount of mushroom polysaccharide, can strengthen The immunologic function of human body.Its unsaturated fatty acid is combined into cholesterol ester with the cholesterol in blood of human body, reduces blood pressure, prevents The only effect of arteriosclerosis.Often eat Pleurotus nebrodensis also disappear disease dissipating blood stasis and clearing away heat removing toxic substances effect.The surprising nutritive value of Pleurotus nebrodensis, Cause the very big concern of scientific circles with health care.
Morchella esculenta (L.) Perss are under the jurisdiction of Ascomycotina (Ascomycotina), discomycete, Pezizale, Morchellaceae, morchella, Gaster caprae seu Ovis Bacterium has many small ruts with its cap surface, gains the name like Gaster caprae seu Ovis in outward appearance pole.It is a kind of wild famous and precious edible and medical fungi, earliest It is embodied in Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》.The traditional Chinese medical science is used as medicine with Morchella esculenta (L.) Pers sporophore.Morchella esculenta (L.) Perss are foremost delicious edible in ascomycetess Bacterium, Morchella esculenta (L.) Perss contain protein 22.1%, fat 3.8%, polysaccharide 16.3%;And the unsaturated fatty acid in fat and saturated fat The ratio of acid is 5 to 3, and the linoleic acid beneficial to human body accounts for the 56% of fatty acid total amount.Aminoacid needed by human is complete, and it contains Amount is 5 times of Hericium erinaceus (Bull. Ex Fr.) Pers..Also contain the necessary elements of human body such as abundant potassium, phosphorus, calcium, ferrum, magnesium, zinc and selenium.Wherein potassium, The content of phosphorus is 4 times and 2 times of Cordyceps respectively, and Zn content is 9 times of Cordyceps.Selenium then can change carcinogenic generation Thank direction and antioxidation, thus Morchella esculenta (L.) Perss are described as " anticancer star ".
Morchella esculenta (L.) Perss are mild-natured, sweet in the mouth tremble with fear, nontoxic, have beneficial stomach, digestion help food, phlegm-eliminiating and qi-regulating, the kidney invigorating, tonifying YANG, supplementing the brain, The function of refreshing oneself.Research finds that Morchella esculenta (L.) Perss have blood fat reducing, adjust immunity, resisting fatigue, anti-radiation, antitumor action, and energy Mitigate the toxic and side effects that cancer patient's chemicotherapy causes.
At present the research of Mushroom production, mushroom processing is increasingly subject to pay attention to,《Food and biotechnology journal》The The 2nd phase of volume 26 in March, 2007《Study on Liquid Fermentation Technology of Morchella esculenta》Morchella esculenta (L.) Perss liquid submerged fermentation is made to dissolve in and has ground Study carefully, the best medium proportioning of selection is:Semen Maydis powder 4.0g/dL, glucose 1.0g/dL, analysis for soybean powder 2.0g/dL, yeast powder 0.3g/dL、KH2PO40.2g/dL、MgSO40.1g/dL、CaSO40.1g/dL;Select optimal culture conditions be:24 DEG C, initiate The shaking flask liquid amount of pH5.8,250mL is 100mL, inoculum concentration 10mL, shaking flask rotating speed 140r/min, and fermentation time is 108h. On market, the species of mushroom converted productss is concentrated mainly on fresh goods, dry productss and three aspects of canned pack, Publication No. The Chinese invention of CN1136408A《Mushroom powder and its production technology》Disclose the edible mushroom powder in solid powdery, produce Technique is:Fresh mushroom, rinsing, broken, cell wall breaking, separation, ultrafiltration, drying.Process is simple but nutrition and delicate flavour composition It is not fine for retaining.
The Chinese invention of Publication No. CN101617808A《A kind of compound mushroom flavoring and preparation method thereof》Disclose A kind of primary raw material is the flavouring agent of agaricus bisporus powder, Volvariella volvacea (Bull.Ex Franch.) Singer. powder, sodium glutamate etc., and the preparation method of wherein mushroom powder is:Will Fresh mushroom rinsing, cooking, pulverizing, defibrination, after homogenizing more concentrated, be spray-dried and be obtained.There is also raw material extraction and application Rate is not high, and nutrition and delicate flavour retain bad problem.
Publication No. CN101455332A Chinese invention《A kind of edible fungus composition of disease preventing and treating》Disclosing one kind can be effective Disease preventing and treating, the edible fungus composition taking good care of one's health, comprise the following raw materials by weight percent:Auricularia 8-10%, Volvariella volvacea (Bull.Ex Franch.) Singer. 9-11%, Mushroom 7-9%, Cordycepses 9-11%, Flammulina velutiper (Fr.) Sing 11-13%, Tremella 10-12%, Cordycepses 12-14%, Pleurotus ostreatus 7-9%, hedgehog hydnum 8-10%, Spice 2-3%, flavoring agent 2-3% and water 4-6%, total amount 100%.First by Auricularia, Volvariella volvacea (Bull.Ex Franch.) Singer., mushroom, Cordycepses, Flammulina velutiper (Fr.) Sing, Tremella, Cordycepses, Pleurotus ostreatus and hedgehog hydnum are cut into bar or block, are subsequently adding spice, flavoring agent and water, place in pressure cooker, high Warm 120-150 DEG C, steaming and decocting 5-10 minute, let cool, tinning or bottling or pack, you can.
The Chinese invention of Publication No. CN103494180A《A kind of anti-tumor healthcare mushroom porridge》Provide a kind of anti-tumor healthcare The processing technology of mushroom porridge.This mushroom porridge include Hericium erinaceus (Bull. Ex Fr.) Pers., northern Morchella esculenta (L.) Perss, Flammulina velutiper (Fr.) Sing, Cordycepses, Tremella, Pleurotus ostreatus, Pleurotus eryngii, Seafood mushroom, crab flavour mushroom, Fructus Lycopersici esculenti, Semen Coicis, rice etc., each raw material is allocated in proportion, selected ingredient requirement green high quality, no Pest and disease damage.Preparation flow includes screening and pretreatment, steaming and decocting, tinning, sterilizing etc. of raw material, and manufacturing process is without any anti- Rotten agent and food additive.It is characterized as:With there is antitumor efficacy edible fungi as primary raw material, and raw material is fresh edible Mushroom entity, is used with antitumor vegetable, corn collocation, enhances value and the nutritive value of various raw materials.This mushroom Medicated porridge comprises various active material such as Gaster caprae seu Ovis rhzomorph, Flammulin, cordycepin etc., and mushroom taste is strong, nutritious, to enhancing immunity, Prophylaxis of tumours has obvious prevention and health-care effect.This technological operation is simple, suitable factorial praluction.
The Chinese invention of Publication No. CN101238878A《Instant edible mushroom and preparation method thereof》Provide a kind of instant edible The preparation method of bacterium, is characterised by that the flavouring agent using includes mushroom powder, this mushroom powder includes Tricholoma matsutake (lto et lmai) Singer dry powder 9-11 by mass parts, Boletus luridus dry powder 18-22, Lentinus Edodess dry powder 9-11, sarcodon aspratus dry powder 9-11, Morchella esculenta (L.) Perss dry powder 9-11, Termitomyces albuminosus dry powder 9-11, Boletus aereus dry powder 90-110, Hericium erinaceus (Bull. Ex Fr.) Pers. dry powder 9-11. have preparation method technological process simply, with low cost;That makes is instant Products taste is excellent, non-greasy, has the characteristics that strong mushroom is natural fresh fragrant.Described mushroom powder is by corresponding fresh feed bacterium Natural air drying grinds after being less than 12% to water content and obtains, granularity 40-120 mesh.
The number of applying for a patent CN01141724.2 discloses the Chinese patent Shen of entitled healthy effervescent tablet and preparation method thereof Please, its feature is the functional oligose containing tool bacillus bifiduss proliferation function, such as oligomeric xylose, oligofructose, low Poly- galactose, oligomeric isomaltose, soybean oligo saccharide etc., and medicine, vitamin, mineral, solubility first can be added The several functions factor such as shell element.This tablet does not adopt traditional wet granulation, and by supplementary material cross 0.4-1.2mm screen cloth, Mix, after 55 65 DEG C of dryings, direct compression produces, and process is simple, energy-conservation, saving of labor, saves time, Factory Building takies face Long-pending little.Its finished product put in water can " grow grow " bubbling, roll up and down, momentariness dissolve into one glass of cup beverage, health product, Medicated tea etc., is rich in interest.
The patent No.:201010268066 disclose a kind of red jujube polysaccharide effervescent tablets and preparation method thereof, and this effervescent tablet is by following The component of weight portion is made:Red jujube polysaccharide 10-20 part, disintegrating agent 20-40 part, diluent 40-100 part, lubricant 0.5-2 Part, correctivess 0-20 part.The step that the present invention prepares red jujube polysaccharide effervescent tablets is as follows:(1) take red jujube polysaccharide and diluent, After correctivess mix homogeneously, add ethanol to moisten, make soft material;(2) pelletize, is dried, granulate;(3) add disintegrating agent, After correctivess, mix lubricant are uniform, compression molding.Product of the present invention has defying age, antioxidation, improves human body immunity energy Power etc. acts on, and health care is good, and suitable various people eat, easy for carrying and eating.A kind of red jujube polysaccharide effervescent tablets, it is special Levy and be:Described effervescent tablet is made up of the component of following parts by weight:Red jujube polysaccharide 10-20 part, disintegrating agent 20-40 part, dilute Release agent 40-100 part, lubricant 0.5-2 part, correctivess 0-20 part.
How to make full use of the feature that mushroom is nutritious, delicious in taste, rich in various nutritional labelings, improve raw material extraction, utilization rate, Exploitation has the flavoring products of good nutrition health care, meets and material requisite that abundant people are growing, developing food Use bacterium new application, be edible fungi field of deep problem demanding prompt solution.
Content of the invention:
Present invention solves the technical problem that being to provide a kind of Chinese caterpillar fungus health powder effervescent tablet products and preparation method thereof.
Technical scheme is summarized as follows:It is made up of the component of following weight portion:
Morchella esculenta (L.) Perss digest powder 10-20 part, and Pleurotus nebrodensis Cordycepses digest powder 15-20 part, and animal proteinum hydrolyzes powder 2-5 part, worm summer in winter 2~5 parts of careless mycelium powder, Fructus Crataegi powder 2-5 part, 20~40 parts of disintegrating agent, 30~80 parts of diluent, 0.5~2 part of lubricant, 2~10 parts of correctivess.
Described disintegrating agent is sodium bicarbonate, and described diluent is dextrin, and described lubricant is magnesium stearate;
Correctivess are sweeting agent and acidic flavoring agent, and sweeting agent is erythritol or xylitol;Acidic flavoring agent is citric acid;
The mass ratio of sweeting agent and acidic flavoring agent is 1:1.1-1.5;
The preparation method of effervescent tablet of the present invention is as follows:(1) Morchella esculenta (L.) Perss enzymolysis powder, Chinese caterpillar fungus bacterial filament powder, Pleurotus nebrodensis Cordycepses enzyme are taken Xie Fen, after animal proteinum hydrolysis powder, Fructus Crataegi powder are mixed homogeneously with diluent, correctivess, adds ethanol to moisten, makes soft material; (2) pelletize, is dried, granulate;(3) after adding disintegrating agent, mix lubricant uniform, compression molding, piece weight 2-4g.
The preparation process that described Morchella esculenta (L.) Perss digest powder is as follows:The fermented method of Morchella esculenta (L.) Perss prepares, and adds fermentation liquid weight 0.5-2% Cellulase degradation process, 30~50 DEG C of temperature, pH4~5, time 0.5-1 hour;Add fermentation liquid weight 0.3-1% Flavor protease enzymolysis processing, 45-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour;Adjustment temperature to 90-110 DEG C Keep 5-10 minute, cooling;Enzymolysis solution is dried and obtains Morchella esculenta (L.) Perss enzymolysis powder.
Chinese caterpillar fungus bacterial filament powder preparation process is as follows:After Cordycepses and Pleurotus nebrodensis sporophore, Radix Isatidis are pulverized, ground product and water are with 1: The ratio mixing of 3-6, adjustment temperature is 40-60 DEG C, and pH is 5.0-7.0, adds Cordycepses and Pleurotus nebrodensis sporophore quality 0.1-0.3% Cellulase degradation 1-1.5 hour after adjustment temperature arrive 80-95 DEG C of holding 2-5 minute, adjustment temperature to 50 DEG C, subsequently interpolation Cordycepses and Pleurotus nebrodensis sporophore quality 0.1-0.3% flavor protease are digested, 40-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis Time 1-2 hour, centrifugation obtains supernatant, and supernatant is spray-dried and obtains Pleurotus nebrodensis Cordycepses enzymolysis powder.Described Cordycepses, Pleurotus nebrodensis sporophore, Radix Isatidis and Radix Angelicae Sinensis are all crushed to particle diameter in below 0.5mm.Cordycepses, Pleurotus nebrodensis, the weight portion of Radix Isatidis Number is respectively Cordycepses 5-8, Pleurotus nebrodensis 10-20, Radix Isatidis 0.1-0.2;
Animal proteinum hydrolysis powder, preparation method thereof is as follows:Quantitative animal flesh is taken to rub through meat grinder, the animal flesh after rubbing and water are with 1: The ratio of 5.1-6 mixes and through milling treatment of colloid, the gap adjusting colloid mill stator with rotor is 105-120 micron, colloid mill stream Amount is controlled to 0.2-0.5 ton hour;Animal flesh adjustment temperature after milling treatment of colloid is 40-60 DEG C, pH is 5.0-7.0, Add the papain enzymolysis 60-100 minute of 0.12-0.25%, be cooled to 5-10 DEG C of holding 1.5-2 hour, increase the temperature to 85-95 DEG C of holding 10-25 minute, controls temperature to 40-50 DEG C, adds animal meat quality 0.12-0.25% flavor protease and carries out Enzymolysis, 40-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, take centrifugation supernatant Liquid (centrifugal rotational speed 3000-5000r/min)) it is spray-dried acquisition animal proteinum hydrolysis powder.
Preferably, described animal flesh is Carnis Gallus domesticus;
The enzyme preparation added in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
Add Radix Achyranthis Bidentatae and the Cortex Eucommiae enzymolysis solution of 10-20% in toadstool ferment culture medium;
Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis liquid and preparation method thereof are as follows:The interpolation mass ratio of Radix Achyranthis Bidentatae and the Cortex Eucommiae is 1:2-3, both were pulverized 100 Mesh sieve, crushed mixture plus 4-8 times of water dissolution adds the cellulase of mixture quality 2-2.5%, adjustment temperature is 40-60 DEG C, PH is 5.0-7.0, keeps 30~60 minutes, obtains Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution.
Beneficial effect
This invention product has mushroom fragrance and projects, and taste is suitable, nutritious feature, is to travel the good merchantable brand of indispensability at home.Logical Cross enzymolysis processing so as to protein obtains a certain degree of degraded in sporophore tissue, measured using amino acid analyzer, Total amino acid content improves 20-37% than before enzymolysis, effectively raises various aminoacid and other flavor substances in product of the present invention Content, make product of the present invention delicious in taste, simultaneously because enzymolysis produce various nutritional labelings good compatibility make the present invention produce Product not only have good seasoning function but also have good nutrition nourishing function.
Animal proteinum hydrolyzes the preparation of powder, is combined using homogenizing, enzymolysis mid-term high-temperature process subsection enzymolysis handling process, is beneficial to Enzyme plays a role, and improves enzymolysis efficiency and nutrient substance dissolution, improves nutritive value and the good taste of product, this product exists Without good mouthfeel under conditions of flavoring agent, taste appraisal score value uses flavouring product higher than related category.
This product local flavor is delicious, rich in multiple trophic function compositions, is a kind of nutrition of collection, nourishing, adjusts body immunity function Edible fungi deep processed product.This product instant, can eat as common food flavouring agent or bottom material of chafing dish, also can be direct Edible.
Specific embodiment:
The following examples can make those skilled in the art that the present invention is more fully understood, but limits the present invention never in any form.
Embodiment 1:A kind of Chinese caterpillar fungus health powder effervescent tablet products and preparation method thereof
Technical scheme is summarized as follows:
The component of following weight portion is made:
Morchella esculenta (L.) Perss digest powder 15, and Pleurotus nebrodensis Cordycepses digest powder 18, and animal proteinum hydrolyzes powder 3,2 parts of Chinese caterpillar fungus bacterial filament powder, mountain 2 parts of short, bristly hair or beard powder, 30 parts of disintegrating agent, 50 parts of diluent, 1 part of lubricant, 5 parts of correctivess;
Described disintegrating agent is sodium bicarbonate, and described diluent is dextrin, and described lubricant is magnesium stearate;
Correctivess are sweeting agent and acidic flavoring agent, and sweeting agent selects erythritol;Acidic flavoring agent is citric acid;
The mass ratio of sweeting agent and acidic flavoring agent is 1:1.2;
The preparation method of effervescent tablet of the present invention is as follows:(1) Morchella esculenta (L.) Perss enzymolysis powder, Chinese caterpillar fungus bacterial filament powder, Pleurotus nebrodensis Cordycepses enzyme are taken Xie Fen, after animal proteinum hydrolysis powder, Fructus Crataegi powder are mixed homogeneously with diluent, correctivess, adds ethanol to moisten, makes soft material; (2) pelletize, is dried, granulate;(3) after adding disintegrating agent, mix lubricant uniform, compression molding, piece weight 3g.
The preparation process that described Morchella esculenta (L.) Perss digest powder is as follows:The fermented method of Morchella esculenta (L.) Perss prepares, and adds the fibre of fermentation liquid weight 1% The plain enzyme enzymolysis processing of dimension, 40 DEG C of temperature, pH4.5,0.8 hour time;Add the flavor protease enzyme of fermentation liquid weight 0.7% Solution is processed, 48 DEG C of hydrolysis temperature, pH value 6.5, enzymolysis time 1.5 hours;Adjustment temperature to 100 DEG C keeps 10 minutes, fall Temperature;Enzymolysis solution is dried and obtains Morchella esculenta (L.) Perss enzymolysis powder.
Chinese caterpillar fungus bacterial filament powder preparation process is as follows:After Cordycepses and Pleurotus nebrodensis sporophore, Radix Isatidis are pulverized, ground product and water are with 1: 5 ratio mixing, adjustment temperature is 55 DEG C, and pH is 6, adds the cellulose enzyme of Cordycepses and Pleurotus nebrodensis sporophore quality 0.2% Solution adjusts temperature to 90 DEG C and keeps 5 minutes after 1.5 hours, adjust temperature to 50 DEG C, subsequently add Cordycepses and Pleurotus nebrodensis sporophore Quality 0.2% flavor protease is digested, 45 DEG C of hydrolysis temperature, pH value 6, enzymolysis time 2 hours, in centrifugation acquisition Clear liquid, supernatant is spray-dried and obtains Pleurotus nebrodensis Cordycepses enzymolysis powder.Described Cordycepses, Pleurotus nebrodensis sporophore, Radix Isatidis and Radix Angelicae Sinensis are equal It is crushed to particle diameter in below 0.5mm.Cordycepses, Pleurotus nebrodensis, the parts by weight of Radix Isatidis are respectively Cordycepses 8, Pleurotus nebrodensis 15, plate Blue root 0.2;
Animal proteinum hydrolysis powder, preparation method thereof is as follows:Quantitative animal flesh is taken to rub through meat grinder, the animal flesh after rubbing and water are with 1: 5.5 ratio mixes and through milling treatment of colloid, and the gap adjusting colloid mill stator with rotor is 110 microns, colloid mill flow-control For 0.4 ton hour;Animal flesh adjustment temperature after milling treatment of colloid is 55 DEG C, pH is 6.0, adds 0.2% Fructus Chaenomeliss egg White enzyme digests 90 minutes, is cooled to 8 DEG C and keeps 1.5 hours, increases the temperature to 90 DEG C and keeps 15 minutes, controls temperature to 45 DEG C, Add animal meat quality 0.2% flavor protease to be digested, 48 DEG C of hydrolysis temperature, pH value 6.2, enzymolysis time 2 hours, obtain To animal protein factor digest, take centrifuged supernatant (centrifugal rotational speed 4500r/min)) it is spray-dried acquisition animal proteinum hydrolysis powder.
Described animal flesh is Carnis Gallus domesticus;
The enzyme preparation added in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
Add 15% Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution in toadstool ferment culture medium;
Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis liquid and preparation method thereof are as follows:The interpolation mass ratio of Radix Achyranthis Bidentatae and the Cortex Eucommiae is 1:2.5, both were pulverized 100 Mesh sieve, crushed mixture plus 6 times of water dissolutioies adds the cellulase of mixture quality 2.5%, adjustment temperature is 50 DEG C, pH For 6.0, keep 50 minutes, obtain Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution.
The preparation of described fermented morel liquid:Spread cultivation step by step using slant strains and obtain Morchella esculenta (L.) Perss fungal fermented filtrate;
(1) first order seed culture:Morchella esculenta (L.) Perss slant strains are accessed in 500 milliliters of shaking flasks, 100 milliliters of fermentation medium loading amount, 180 revs/min of rotary shaker, 24 DEG C of cultivation temperature, incubation time 50 hours;
(2) secondary seed culture:First order seed is accessed in 500 milliliters of secondary seed shaking flasks according to 10% inoculum concentration, culture Condition is identical with first order seed;
(3) three-level seed culture:Secondary seed is accessed in 5000 milliliters of three-level seed flask with 10% inoculum concentration, fermentation training 1000 milliliters of foster base loading amount, 100 revs/min of rotary shaker, 24 DEG C of cultivation temperature, incubation time 50 hours;
(4) first class seed pot culture:Three-level seed is accessed the first class seed pot as 150L for the total measurement (volume) with 10% inoculum concentration, sends out Ferment culture medium loading amount 100L, 24 DEG C of cultivation temperature, 150 revs/min of mixing speed, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, Incubation time 48 hours;
(5) secondary seed tank culture:First class seed pot strain is accessed total measurement (volume) for 1.5 tons of secondary seed tanks with 10% inoculum concentration, 1 ton of fermentation medium loading amount, condition of culture is identical with first class seed pot;
(6) fermentor cultivation:Strain in secondary seed tank is accessed, with 10% inoculum concentration, the fermentation tank that total measurement (volume) is 13 tons, 10 tons of fermentation medium loading amount, 24 DEG C of cultivation temperature, 100 revs/min of mixing speed, ventilation (V/V) 1:0.4, tank pressure 0.05 Mpa, incubation time 72 hours.
Described above-mentioned fermented morel liquid fermentation medium consists of:Semen setariae 2%, peptone 1%, Carnis Bovis seu Bubali cream 1%, Semen Maydiss, 2.5%, Potassium dihydrogen phosphate 0.5%, 15% Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution;Insufficient section water is supplied, pH7.0-7.2,121 DEG C of high steams Sterilizing 20min.
The enzyme preparation added in the present invention is purchased from letter enzyme preparation company of Novi.
Described Morchella esculenta (L.) Perss are purchased from China General Microbiological culture presevation administrative center, numbering CGMCC NO:5.621.
Embodiment 2:A kind of Chinese caterpillar fungus health powder effervescent tablet products and preparation method thereof
Technical scheme is summarized as follows:
The component of following weight portion is made:
Morchella esculenta (L.) Perss digest powder 10, and Pleurotus nebrodensis Cordycepses digest powder 20, and animal proteinum hydrolyzes powder 5,4 parts of Chinese caterpillar fungus bacterial filament powder, mountain 3 parts of short, bristly hair or beard powder, 25 parts of disintegrating agent, 40 parts of diluent, 0.5 part of lubricant, 3 parts of correctivess;
Described disintegrating agent is sodium bicarbonate, and described diluent is dextrin, and described lubricant is magnesium stearate;
Correctivess are sweeting agent and acidic flavoring agent, and sweeting agent selects xylitol;Acidic flavoring agent is citric acid;
The mass ratio of sweeting agent and acidic flavoring agent is 1:1.1;
Effervescent piece preparation method is with embodiment 1;
The preparation process that described Morchella esculenta (L.) Perss digest powder is as follows:The cellulase degradation adding fermentation liquid weight 2% is processed, temperature 45 C, PH4.0,1 hour time;Add the flavor protease enzymolysis processing of fermentation liquid weight 0.4%, 50 DEG C of hydrolysis temperature, pH value 6.0, enzymolysis time 1 hour;Adjustment temperature to 95 DEG C keeps 8 minutes, cooling;Enzymolysis solution is dried and obtains Morchella esculenta (L.) Perss enzymolysis powder.
Chinese caterpillar fungus bacterial filament powder preparation process is as follows:After Cordycepses and Pleurotus nebrodensis sporophore, Radix Isatidis are pulverized, ground product and water are with 1: 6 ratio mixing, adjustment temperature is 40 DEG C, and pH is 7, adds the cellulose enzyme of Cordycepses and Pleurotus nebrodensis sporophore quality 0.3% Solution adjusts temperature to 85 DEG C and keeps 2 minutes after 1 hour, adjust temperature to 50 DEG C, subsequently add Cordycepses and Pleurotus nebrodensis sporophore matter Measure 0.3% flavor protease to be digested, 50 DEG C of hydrolysis temperature, pH value 7, enzymolysis time 1 hour, centrifugation obtains supernatant Liquid, supernatant is spray-dried and obtains Pleurotus nebrodensis Cordycepses enzymolysis powder.Described Cordycepses, Pleurotus nebrodensis sporophore, Radix Isatidis and the equal powder of Radix Angelicae Sinensis It is broken to particle diameter in below 0.5mm.Cordycepses, Pleurotus nebrodensis, the parts by weight of Radix Isatidis are respectively Cordycepses 5, Pleurotus nebrodensis 20, Baphicacanthus cusia Root 0.1;
Animal proteinum hydrolysis powder, preparation method thereof is as follows:Quantitative animal flesh is taken to rub through meat grinder, the animal flesh after rubbing and water are with 1: 6 ratio mixes and through milling treatment of colloid, and the gap adjusting colloid mill stator with rotor is 105 microns, colloid mill flow-control For 0.5 ton hour;Animal flesh adjustment temperature after milling treatment of colloid is 60 DEG C, pH is 7.0, adds 0.25% Fructus Chaenomeliss Protease hydrolyzed 95 minutes, is cooled to 10 DEG C and keeps 2 hours, increases the temperature to 95 DEG C and keeps 25 minutes, controls temperature to arrive 48 DEG C, add animal meat quality 0.25% flavor protease and digested, 50 DEG C of hydrolysis temperature, pH value 7, enzymolysis time 2 is little When, obtain animal protein factor digest, take centrifuged supernatant (centrifugal rotational speed 3500r/min)) it is spray-dried acquisition animal proteinum hydrolysis powder.
Described animal flesh is Carnis Gallus domesticus;
The enzyme preparation added in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
Add 20% Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution in toadstool ferment culture medium;
Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis liquid and preparation method thereof are as follows:The interpolation mass ratio of Radix Achyranthis Bidentatae and the Cortex Eucommiae is 1:3, both were pulverized 100 Mesh sieve, crushed mixture plus 8 times of water dissolutioies adds the cellulase of mixture quality 2.5%, adjustment temperature is 60 DEG C, pH For 7.0, keep 55 minutes, obtain Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution.
The preparation process of described fermented morel liquid such as embodiment 1.
Wherein fermented morel liquid fermentation medium consists of:Semen setariae 2%, peptone 1%, Carnis Bovis seu Bubali cream 1%, Semen Maydiss, 2.5%, Potassium dihydrogen phosphate 0.5%, 20% Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution;Insufficient section water is supplied, pH7.0-7.2,121 DEG C of high steams Sterilizing 20min.
The enzyme preparation added in the present invention is purchased from letter enzyme preparation company of Novi.
Described Morchella esculenta (L.) Perss are purchased from China General Microbiological culture presevation administrative center, numbering CGMCC NO:5.621.
Embodiment 3:A kind of Chinese caterpillar fungus health powder effervescent tablet products and preparation method thereof
Technical scheme is summarized as follows:
The component of following weight portion is made:
Morchella esculenta (L.) Perss digest powder 20, and Pleurotus nebrodensis Cordycepses digest powder 15, and animal proteinum hydrolyzes powder 2,3 parts of Chinese caterpillar fungus bacterial filament powder, mountain 4 parts of short, bristly hair or beard powder, 35 parts of disintegrating agent, 75 parts of diluent, 2 parts of lubricant, 8 parts of correctivess;
Described disintegrating agent is sodium bicarbonate, and described diluent is dextrin, and described lubricant is magnesium stearate;
Correctivess are sweeting agent and acidic flavoring agent, and sweeting agent selects xylitol;Acidic flavoring agent is citric acid;
The mass ratio of sweeting agent and acidic flavoring agent is 1:1.5;
Effervescent piece preparation method is with embodiment 1;
The preparation process that described Morchella esculenta (L.) Perss digest powder is as follows:The cellulase degradation adding fermentation liquid weight 0.5% is processed, temperature 50 DEG C, pH5.0,0.5 hour time;The flavor protease enzymolysis processing of interpolation fermentation liquid weight 1%, 45 DEG C of hydrolysis temperature, PH value 7.0, enzymolysis time 2 hours;Adjustment temperature to 90 DEG C keeps 5 minutes, cooling;Enzymolysis solution is dried and obtains Morchella esculenta (L.) Perss enzyme Xie Fen.
Chinese caterpillar fungus bacterial filament powder preparation process is as follows:After Cordycepses and Pleurotus nebrodensis sporophore, Radix Isatidis are pulverized, ground product and water are with 1: 3 ratio mixing, adjustment temperature is 60 DEG C, and pH is 5, adds the cellulose enzyme of Cordycepses and Pleurotus nebrodensis sporophore quality 0.1% Solution adjusts temperature to 80 DEG C and keeps 3 minutes after 1.2 hours, adjust temperature to 50 DEG C, subsequently add Cordycepses and Pleurotus nebrodensis sporophore Quality 0.1% flavor protease is digested, 40 DEG C of hydrolysis temperature, pH value 6.5, enzymolysis time 1.5 hours, and centrifugation obtains Obtain supernatant, supernatant is spray-dried and obtains Pleurotus nebrodensis Cordycepses enzymolysis powder.Described Cordycepses, Pleurotus nebrodensis sporophore, Radix Isatidis and work as Gui Jun is crushed to particle diameter in below 0.5mm.Cordycepses, Pleurotus nebrodensis, Radix Isatidis parts by weight be respectively Cordycepses 7, Pleurotus nebrodensis 10, Radix Isatidis 0.15;
Animal proteinum hydrolysis powder, preparation method thereof is as follows:Quantitative animal flesh is taken to rub through meat grinder, the animal flesh after rubbing and water are with 1: 5.1 ratio mixes and through milling treatment of colloid, and the gap adjusting colloid mill stator with rotor is 120 microns, colloid mill flow-control For 0.2 ton hour;Animal flesh adjustment temperature after milling treatment of colloid is 42 DEG C, pH is 5.0, adds 0.12% Fructus Chaenomeliss Protease hydrolyzed 65 minutes, is cooled to 5 DEG C and keeps 1.8 hours, increases the temperature to 85 DEG C and keeps 10 minutes, controls temperature to arrive 40 DEG C, add animal meat quality 0.15% flavor protease and digested, 40 DEG C of hydrolysis temperature, pH value 6, enzymolysis time 1 is little When, obtain animal protein factor digest, take centrifuged supernatant (centrifugal rotational speed 5000r/min)) it is spray-dried acquisition animal proteinum hydrolysis powder.
Described animal flesh is Carnis Gallus domesticus;
The enzyme preparation added in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
Add 10% Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution in toadstool ferment culture medium;
Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis liquid and preparation method thereof are as follows:The interpolation mass ratio of Radix Achyranthis Bidentatae and the Cortex Eucommiae is 1:2, both were pulverized 100 Mesh sieve, crushed mixture plus 4 times of water dissolutioies adds the cellulase of mixture quality 2%, adjustment temperature is 40 DEG C, pH For 5.0, keep 30 minutes, obtain Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution.
The preparation process of described fermented morel liquid such as embodiment 1.
Wherein fermented morel liquid fermentation medium consists of:Semen setariae 2%, peptone 1%, Carnis Bovis seu Bubali cream 1%, Semen Maydiss, 2.5%, Potassium dihydrogen phosphate 0.5%, 10% Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution;Insufficient section water is supplied, pH7.0-7.2,121 DEG C of high steams Sterilizing 20min.
The enzyme preparation added in the present invention is purchased from letter enzyme preparation company of Novi.
Described Morchella esculenta (L.) Perss are purchased from China General Microbiological culture presevation administrative center, numbering CGMCC NO:5.621.
Embodiment 4:Product effect experimental
Weigh each raw material by formula, microwave disinfection after mix homogeneously, packaging is product.
With embodiment 1 products obtained therefrom as sample, select in the winter time 100 ages of Efficiency in Buildings in Tianjin Area 60-75 year weak easy catching a cold Old people continuously eats January, edible 2 daily, every 3 grams, with edible front and edible rear effectiveness comparison, the results are shown in Table 1, Result shows that this product has positive effect to raising old people's resistance, enhancing body immunity, and product has good edible and battalion Foster nourishing effects.Many continuously eats this product old man and reflects that long term usage is better, and whole year has no interest, and health is more preferable.
Table 1 eating effect synopsis
Number Edible first 30 days times of common cold (total) Times of common cold (total) in 30 days after edible 2 months
100 30 8

Claims (6)

1. a kind of Chinese caterpillar fungus health powder effervescent tablet is it is characterised in that be grouped into by the one-tenth of following parts by weight:Morchella esculenta (L.) Perss digest powder 10-20 part, and Pleurotus nebrodensis Cordycepses digest powder 15-20 part, and animal proteinum hydrolyzes powder 2-5 part, 2~5 parts of Chinese caterpillar fungus bacterial filament powder, Fructus Crataegi powder 2-5 part, 20~40 parts of disintegrating agent, 30~80 parts of diluent, 0.5~2 part of lubricant, 2~10 parts of correctivess;
The preparation process that described Morchella esculenta (L.) Perss digest powder is as follows:The fermented method of Morchella esculenta (L.) Perss prepares, and the cellulase degradation adding fermentation liquid weight 0.5-2% is processed, 30~50 DEG C of temperature, pH4~5, time 0.5-1 hour;Add the flavor protease enzymolysis processing of fermentation liquid weight 0.3-1%, 45-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour;Adjustment temperature is to 90-110 DEG C of holding 5-10 minute, cooling;Enzymolysis solution is dried and obtains Morchella esculenta (L.) Perss enzymolysis powder;
Add Radix Achyranthis Bidentatae and the Cortex Eucommiae enzymolysis solution of 10-20% in the fermentation medium of described Morchella esculenta (L.) Perss;Described Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis liquid and preparation method thereof are as follows:The interpolation mass ratio of Radix Achyranthis Bidentatae and the Cortex Eucommiae is 1:2-3, both were pulverized 100 mesh sieves, crushed mixture plus 4-8 times of water dissolution added the cellulase of mixture quality 2-2.5%, adjustment temperature is 40-60 DEG C, and pH is 5.0-7.0, keeps 30~60 minutes, obtains Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution.
2. Chinese caterpillar fungus health powder effervescent tablet as claimed in claim 1 is it is characterised in that the preparation method of described Chinese caterpillar fungus health powder effervescent tablet is as follows:(1) take Morchella esculenta (L.) Perss enzymolysis powder, Chinese caterpillar fungus bacterial filament powder, Pleurotus nebrodensis Cordycepses enzymolysis powder, after animal proteinum hydrolysis powder, Fructus Crataegi powder are mixed homogeneously with diluent, correctivess, add ethanol to moisten, make soft material;(2) pelletize, is dried, granulate;(3) after adding disintegrating agent, mix lubricant uniform, compression molding, piece weight 2-4g.
3. Chinese caterpillar fungus health powder effervescent tablet as claimed in claim 1 is it is characterised in that described Pleurotus nebrodensis Cordycepses enzymolysis powder preparation process is as follows:After Cordycepses and Pleurotus nebrodensis sporophore, Radix Isatidis are pulverized, ground product and water are with 1:The ratio mixing of 3-6, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, after adding Cordycepses and the cellulase degradation 1-1.5 hour of Pleurotus nebrodensis sporophore quality 0.1-0.3%, adjustment temperature is to 80-95 DEG C of holding 2-5 minute, adjustment temperature to 50 DEG C, subsequently interpolation Cordycepses and Pleurotus nebrodensis sporophore quality 0.1-0.3% flavor protease are digested, 40-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour, centrifugation obtains supernatant, and supernatant is spray-dried and obtains Pleurotus nebrodensis Cordycepses enzymolysis powder;
Described Cordycepses, Pleurotus nebrodensis sporophore, Radix Isatidis are all crushed to particle diameter in below 0.5mm, and Cordycepses, Pleurotus nebrodensis, the parts by weight of Radix Isatidis are respectively Cordycepses 5-8, Pleurotus nebrodensis 10-20, Radix Isatidis 0.1-0.2.
4. Chinese caterpillar fungus health powder effervescent tablet as claimed in claim 1 is it is characterised in that described animal proteinum hydrolysis powder, preparation method thereof is as follows:Quantitative animal flesh is taken to rub through meat grinder, the animal flesh after rubbing and water are with 1:The ratio of 5.1-6 mixes and through milling treatment of colloid, the gap adjusting colloid mill stator with rotor is 105-120 micron, and colloid mill flow-control is 0.2-0.5 ton hour;Animal flesh adjustment temperature after milling treatment of colloid is 40-60 DEG C, pH is 5.0-7.0, add the papain enzymolysis 60-100 minute of 0.12-0.25%, it is cooled to 5-10 DEG C of holding 1.5-2 hour, increase the temperature to 85-95 DEG C of holding 10-25 minute, control temperature to 40-50 DEG C, add animal meat quality 0.12-0.25% flavor protease to be digested, 40-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, take centrifuged supernatant, be spray-dried and obtain animal proteinum hydrolysis powder.
5. it is characterised in that described disintegrating agent is sodium bicarbonate, described diluent is dextrin to Chinese caterpillar fungus health powder effervescent tablet as claimed in claim 1, and described lubricant is magnesium stearate;Described correctivess are sweeting agent and acidic flavoring agent, and described sweeting agent is erythritol or xylitol;Acidic flavoring agent is citric acid;The mass ratio of described sweeting agent and acidic flavoring agent is 1:1.1-1.5.
6. a kind of method preparing Chinese caterpillar fungus health powder effervescent tablet is it is characterised in that be made up of the raw material of following parts by weight:Morchella esculenta (L.) Perss digest powder 10-20 part, and Pleurotus nebrodensis Cordycepses digest powder 15-20 part, and animal proteinum hydrolyzes powder 2-5 part, 2~5 parts of Chinese caterpillar fungus bacterial filament powder, Fructus Crataegi powder 2-5 part, 20~40 parts of disintegrating agent, 30~80 parts of diluent, 0.5~2 part of lubricant, 2~10 parts of correctivess;
Take Morchella esculenta (L.) Perss enzymolysis powder, Chinese caterpillar fungus bacterial filament powder, Pleurotus nebrodensis Cordycepses enzymolysis powder by above-mentioned parts by weight (1), after animal proteinum hydrolysis powder, Fructus Crataegi powder are mixed homogeneously with diluent, correctivess, add ethanol to moisten, make soft material;(2) pelletize, is dried, granulate;(3) after adding disintegrating agent, mix lubricant uniform, compression molding, piece weight 2-4g;
The preparation process that described Morchella esculenta (L.) Perss digest powder is as follows:The fermented method of Morchella esculenta (L.) Perss prepares, and is adjusted to 40-60 DEG C after fermentation ends, and the cellulase degradation adding fermentation liquid weight 0.5-2% is processed, 30~50 DEG C of temperature, pH4~5, time 0.5-1 hour;Add the flavor protease enzymolysis processing of fermentation liquid weight 0.3-1%, 45-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour;Adjustment temperature is to 90-110 DEG C of holding 5-10 minute, cooling;Enzymolysis solution is dried and obtains Morchella esculenta (L.) Perss enzymolysis powder;
Add Radix Achyranthis Bidentatae and the Cortex Eucommiae enzymolysis solution of 10-20% in the fermentation medium of described Morchella esculenta (L.) Perss;Described Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis liquid and preparation method thereof are as follows:The interpolation mass ratio of Radix Achyranthis Bidentatae and the Cortex Eucommiae is 1:2-3, both were pulverized 100 mesh sieves, crushed mixture plus 4-8 times of water dissolution added the cellulase of mixture quality 2-2.5%, adjustment temperature is 40-60 DEG C, and pH is 5.0-7.0, keeps 30~60 minutes, obtains Radix Achyranthis Bidentatae and Cortex Eucommiae enzymolysis solution;
Pleurotus nebrodensis Cordycepses enzymolysis powder preparation process is as follows:After Cordycepses and Pleurotus nebrodensis sporophore, Radix Isatidis are pulverized, ground product and water are with 1:The ratio mixing of 3-6, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, after adding Cordycepses and the cellulase degradation 1-1.5 hour of Pleurotus nebrodensis sporophore quality 0.1-0.3%, adjustment temperature is to 80-95 DEG C of holding 2-5 minute, adjustment temperature to 50 DEG C, subsequently interpolation Cordycepses and Pleurotus nebrodensis sporophore quality 0.1-0.3% flavor protease are digested, 40-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour, centrifugation obtains supernatant, and supernatant is spray-dried and obtains Pleurotus nebrodensis Cordycepses enzymolysis powder;Described Cordycepses, Pleurotus nebrodensis sporophore, Radix Isatidis are all crushed to particle diameter in below 0.5mm;Cordycepses, Pleurotus nebrodensis, the parts by weight of Radix Isatidis are respectively Cordycepses 5-8, Pleurotus nebrodensis 10-20, Radix Isatidis 0.1-0.2;
Animal proteinum hydrolysis powder, preparation method thereof is as follows:Quantitative animal flesh is taken to rub through meat grinder, the animal flesh after rubbing and water are with 1:The ratio of 5.1-6 mixes and through milling treatment of colloid, the gap adjusting colloid mill stator with rotor is 105-120 micron, and colloid mill flow-control is 0.2-0.5 ton hour;Animal flesh adjustment temperature after milling treatment of colloid is 40-60 DEG C, pH is 5.0-7.0, add the papain enzymolysis 60-100 minute of 0.12-0.25%, it is cooled to 5-10 DEG C of holding 1.5-2 hour, increase the temperature to 85-95 DEG C of holding 10-25 minute, control temperature to 40-50 DEG C, add animal meat quality 0.12-0.25% flavor protease to be digested, 40-50 DEG C of hydrolysis temperature, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, take centrifuged supernatant, be spray-dried and obtain animal proteinum hydrolysis powder;
Described disintegrating agent is sodium bicarbonate, and described diluent is dextrin, and described lubricant is magnesium stearate;Described correctivess are sweeting agent and acidic flavoring agent, and described sweeting agent is erythritol or xylitol;Acidic flavoring agent is citric acid;The mass ratio of described sweeting agent and acidic flavoring agent is 1:1.1-1.5.
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CN101263885A (en) * 2008-04-28 2008-09-17 广东省微生物研究所 Edible mushroom flavoring agent and preparation thereof
CN102960819A (en) * 2012-10-21 2013-03-13 山西汉波食品股份有限公司 Haw and red date effervescent tablet

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CN102960819A (en) * 2012-10-21 2013-03-13 山西汉波食品股份有限公司 Haw and red date effervescent tablet

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