CN107475340A - A kind of preparation method of truffle bacterium active peptide - Google Patents
A kind of preparation method of truffle bacterium active peptide Download PDFInfo
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- CN107475340A CN107475340A CN201710869656.0A CN201710869656A CN107475340A CN 107475340 A CN107475340 A CN 107475340A CN 201710869656 A CN201710869656 A CN 201710869656A CN 107475340 A CN107475340 A CN 107475340A
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The present invention relates to traditional Chinese medicine and technical field of biology.It is formed using truffle bacterium that is fresh or drying as raw material through broken, homogenate, enzymolysis, centrifugation, sterilizing, ultrafiltration.The truffle bacterium after being homogenized will be crushed and adjust pH value 68, add cellulase, edible mushroom enzyme, neutral proteinase, papain（1:1:1:2）Digested, every gram of truffle bacterium adds 5,000 10000 enzyme-activity units（u）, temperature control is at 50 60 degrees Celsius, enzymolysis 36 hours；Then enzyme deactivation, centrifugation, sterilizing, ultrafiltration obtain the truffle bacterium active peptide liquid below the dalton of molecular weight 5000；Freeze-dried powder product, oral liquid product, capsule product can be prepared.The truffle bacterium active peptide, which has, tonifies the kidney and support yang, improves the pharmacological activity such as immunity of organisms, anti-aging, antifatigue, antitumor, prevention and adjuvant therapy of heart cerebrovascular diseases, regulation endocrine.Process scientific and precise of the present invention, strong operability, is easy to industrialized production.
Description
Technical field
The present invention relates to traditional Chinese medicine and technical field of biology.Exempt from more particularly, to one kind with tonifying the kidney and support yang, improving body
The truffle bacterium of the effect such as epidemic disease power, anti-aging, antifatigue, antitumor, prevention and adjuvant therapy of heart cerebrovascular diseases, regulation endocrine
The preparation method of active peptide.
Background technology
Truffle (scientific name:Truffle it is) a kind of general name of Mushrooms, is categorized as Ascomycota the West truffle section the West truffle category
(scientific name:Tuber).About 10 kinds of different kinds, typically annual fungi.Majority silk life in the root of broad leaf tree
It is long, typically it is grown under pine tree, robur, Oak Tree.Intersperse among tree bottom and have an area of 120 ~ 150 centimetres, block-shaped main body is hidden in underground 3 ~ 40
Centimetre.It is distributed in the states such as Italy, France, Spain, China, New Zealand.Chinese truffle is mainly produced in Drainage Area of Jinsha River, existing
Multiple truffle kinds such as black pine, kahikatea dew are had found, are one of Jinsha jiang River special products.Have an area of 200 kilometers centered on Panzhihua
In the range of be Chinese truffle major production areas.Because truffle is very fastidious for growing environment, as long as the acid of sunlight, water or soil
Base number varies slightly just growing, this be also why truffle so rare reason.How expensive truffle have on earth, and world's quality is most
Good truffle is to originate from Alba, ITA（Alba）Kahikatea dew, one kilogram of 3.5 ten thousand dollars of once asked price, furthermore be exactly the method for originating from
State Pei Lige black pine, at least also want 500 dollars of per kilograms.The edible smell of truffle is special, contains abundant protein, amino
The nutriments such as acid.Requirement of the truffle to growing environment is extremely harsh, and can not artificial culture, yield is rare, result in its
Rare costliness.Therefore European is " three big precious meat of the world " arranged side by side by truffle and caviar, foie gras.
Black pine contains abundant protein, 18 kinds of amino acid（The 8 kinds of essential amino acids that can not be synthesized including human body）, no
The essential trace elements such as saturated fatty acid, multivitamin, zinc, manganese, iron, calcium, phosphorus, selenium, and sphingolipid, cerebroside, nerve
The substantial amounts of metabolites such as acid amides, triterpene, androsterone, adenosine, truffle acid, sterol, truffle polysaccharide, truffle polypeptide, have high
Healthy nutritive value.Wherein androsterone has supporing yang, endocrine significant effect of conditioning；Sphingolipid compound is preventing old be crazy about
There is obvious activity in terms of slow-witted, atherosclerosis and antitumor cell toxicity；Polysaccharide, polypeptide, triterpene have strengthen immunity,
Anti-aging, fatigue-resisting function, androsterone have supporing yang, the endocrine remarkable efficacy of conditioning；Sphingolipid compound is preventing old age
There is obvious activity in terms of dull-witted, atherosclerosis and antitumor cell toxicity；Polysaccharide, polypeptide, triterpene have enhancing immune
Power, anti-aging, antifatigue effect etc..The presence of these rare native compounds constitutes the material base of the magical effect of black pine
Plinth.History and it is cultural due to, east is to the awareness and understanding of black pine, just as cognition one of the west to cordyceps sinensis
Sample, still in the budding stage.Both rare mushrooms have surprising similarity in respective region institute role in fact,
All it is the representative of extremely expensive luxurious food materials, is all showed with fabulous function, all carries each national long dietotherapy text
Change.
Truffle bacterium has so many benefit to human body in summary, can in the market there is no truffle product sell, have in addition to eating raw
Businessman dries the section of truffle bacterium and commercially sold, and there is raw material serious waste drawback, it is impossible to fundamentally fully sends out
Wave the effect of truffle bacterium disease preventing and treating function.
Active peptide is the general name of more than 1,000 kinds of peptide.It grows people's, metabolism, disease and aging, dead
During play key effect.Active peptide is most important active material in human body.Exactly because its secretory volume in vivo
Increase or reduce, the mankind is had childhood, in childhood, adult is old until the cycle of death.And take active peptide and just break
This cycle of life, so as to reach extending life, the miraculous effects of effective delaying senility.Peptide in vivo as carrier and
Means of transport, by the nutrient delivery that people are eaten usually to each position of human body, give full play to its function；Peptide is made in vivo
For neurotransmitter, transmission information；Peptide has extremely strong activity and diversity, fully adjustable human body physiological function, strengthens human body
Physiologically active；Peptide can not only provide the nutriment needed for growth in humans's development, and have special biological function, can prevent
Control thrombus, high fat of blood, hypertension, anti-aging is antifatigue, improve immunity of organisms, promote human body to protein, vitamin,
A variety of absorptions to human body healthy trace elements with household such as amino acid, calcium, iron, zinc, selenium, magnesium, copper；Some small peptides have former food protein
Matter or other composition unexistent important physiological functions of amino acid.The less small peptide of some molecular weight in biologically active peptide, with
Amino acid transport function phase ratio, the small peptide in biologically active peptide, which has, absorbs quick, low consumption and undersaturated feature.Therefore how
The truffle bacterium of expensive resource scarcity is made into function and absorbs more preferable active peptide, makes full use of raw material preferably to reach
The purpose preventiveed treatment of disease to traditional Chinese medicine, really realizes the modernization of Chinese medicine, it has also become traditional Chinese medicine and technical field of biology are urgently to be resolved hurrily
Problem.
The content of the invention
In order to overcome the shortcomings of existing truffle bacterium it is edible during wastage of material and absorption of human body utilization rate it is low, the present invention
Provide a kind of preparation method of truffle bacterium active peptide, biologically active peptide be rich in the truffle bacterium active peptide, have tonify the kidney and support yang,
Improve immunity of organisms, anti-aging, antifatigue, antitumor, prevention and adjuvant therapy of heart cerebrovascular diseases, regulation endocrine, prevent
The pharmacological activity such as cancer anticancer, and be easy to digest and assimilate, nutritious body utilization rate is high.A kind of truffle bacterium active peptide of the present invention
Preparation method be using various truffle bacterium as primary raw material, conventional formulation and rigorous concocted specification are taken, with modern biotechnology skill
The advanced processing technology of art is prepared, its process scientific and precise, strong operability.
The preparation method of truffle bacterium active peptide of the present invention be using truffle bacterium as raw material, through broken, homogenate, enzymolysis,
The truffle bacterium active peptide liquid that centrifugation, sterilizing form.
The purpose of the present invention is achieved through the following technical solutions：A kind of preparation method of truffle bacterium active peptide, it is special
Different part is：
1st, the preparation of truffle bacterium active peptide：Choose truffle bacterium that is fresh or drying and carry out pulverization process；Addition crushes truffle bacterium weight
5-15 times of water is diluted immersion and stirred for 30 minutes, crushes the truffle bacterium after homogenate and adjusts pH value 6-8, adds cellulose
Enzyme, edible mushroom enzyme, neutral proteinase, papain（1:1:1:2）Multienzyme is digested, and enzyme dosage is that every gram of truffle bacterium is added
5000-10000 enzyme-activity unit（u）, between 50-60 degrees Celsius of temperature control, digest 3-6 hours；Enzymolysis finishes boiling water heating bath
Centrifugation, centrifuge speed 4000-10000rpm or so are cooled down after enzyme deactivation 5-20 minutes, produces truffle bacterium active peptide liquid；
2nd, the truffle bacterium active peptide liquid of above-mentioned preparation is put into stainless steel cask enter steam sterilization cabinet between 100-120 degree Celsius and steam
Vapour autoclaving 15-40 minutes；
3rd, the truffle bacterium active peptide liquid of above-mentioned preparation is filtered into obtain pine below the dalton of molecular weight 5000 by ultrafiltration membrane filter
Reveal bacterium active peptide liquid；
4th, the truffle bacterium active peptide liquid of above-mentioned preparation is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the bottled freeze-dried powder product of truffle bacterium active peptide of the present invention；Pure water is added to press the truffle bacterium active peptide liquid of above-mentioned preparation
Oral liquid process specification is filling to oral liquid bottle, produces truffle bacterium oral liquid of active peptide product of the present invention；By above-mentioned preparation
Truffle bacterium active peptide liquid it is freeze-dried or spray drying be prepared into powdery granule, by capsule manufacturing techniques specification it is canned into
Capsule, produce truffle bacterium active peptide capsule product of the present invention.
The present invention is raw material from truffle bacterium, and a large amount of active peptide components, its nutritive value and suction are generated by controlled enzymatic hydrolysis
Utilization rate and edible safety is received to greatly improve, tonify the kidney and support yang, improve immunity of organisms, anti-aging, it is antifatigue, antitumor,
Pharmacological activity enhancing, prevention and the auxiliary treatment disease such as prevention and adjuvant therapy of heart cerebrovascular diseases, regulation endocrine, cancer-resisting
The effect of sick, also accordingly strengthens, and a kind of truffle bacterium active peptide of the present invention and preparation method thereof is filling lyophilized according to lyophilized technique specification
Freeze dryer was freeze-dried into bottled freeze-dried powder product, the additive-free preservative of vacuum freeze drying, safe, shelf-life on bottle
It is long；Addition pure water is filling into oral liquid product according to oral liquid process specification, and color is natural, distinctive natural wind interest
Sense is good；Truffle bacterium active peptide liquid is spray-dried or is freeze-dried and is prepared into powdery granule, by capsule manufacturing techniques specification
It is filling into capsule product, take easy to carry.Preparation technology of the present invention is rigorous rationally, strong operability, is especially suitable for scale metaplasia
Production.
Embodiment
With reference to embodiment, the present invention will be further described
Embodiment one, 1, the preparation of truffle bacterium active peptide liquid：Choose fresh or dry net truffle bacterium 15kg and carry out pulverization process；
The water of 10 times of addition crushing truffle bacterium weight is diluted immersion and stirred for 30 minutes, crushes the truffle bacterium after homogenate and adjusts pH value
6.5, add cellulase, edible mushroom enzyme, neutral proteinase, papain（1:1:1:2）Multienzyme is digested, and enzyme dosage is
Every gram of truffle bacterium adds 9500 enzyme-activity units（u）, 50 degrees Celsius of temperature control, digest 4.5 hours；Enzymolysis finishes boiling water heating bath
Enzyme deactivation cools down centrifugation, centrifuge speed 9000rpm or so after 10 minutes, produces truffle bacterium active peptide liquid；
2nd, the truffle bacterium active peptide liquid of above-mentioned preparation is put into stainless steel cask enter steam sterilization cabinet and gone out in 100° centigrade vapor injection
Bacterium 40 minutes；
3rd, the truffle bacterium active peptide liquid of above-mentioned preparation is filtered into obtain pine below the dalton of molecular weight 5000 by ultrafiltration membrane filter
Reveal bacterium active peptide liquid；
4th, the truffle bacterium active peptide liquid of above-mentioned preparation is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the bottled freeze-dried powder product of truffle bacterium active peptide of the present invention；Pure water is added to press the truffle bacterium active peptide liquid of above-mentioned preparation
Oral liquid process specification is filling to oral liquid bottle, produces truffle bacterium oral liquid of active peptide product of the present invention；By above-mentioned preparation
Truffle bacterium active peptide liquid it is freeze-dried or spray drying be prepared into powdery granule, by capsule manufacturing techniques specification it is canned into
Capsule, produce truffle bacterium active peptide capsule product of the present invention.
Embodiment two, 1, the preparation of truffle bacterium active peptide liquid：Fresh or dry net truffle bacterium 10kg is chosen to carry out at crushing
Reason；The water of 10 times of addition crushing truffle bacterium weight is diluted immersion and stirred for 30 minutes, crushes the truffle bacterium after homogenate and adjusts
PH value 7, add cellulase, edible mushroom enzyme, neutral proteinase, papain（1:1:1:2）Multienzyme is digested, enzyme dosage
7500 enzyme-activity units are added for every gram of truffle bacterium（u）, 50 degrees Celsius of temperature control, digest 5 hours；Enzymolysis finishes boiling water heating bath
Enzyme deactivation cools down centrifugation, centrifuge speed 9500rpm or so after 10 minutes, produces truffle bacterium active peptide liquid；
2nd, the truffle bacterium active peptide liquid of above-mentioned preparation is put into stainless steel cask enter steam sterilization cabinet and gone out in 105 degree of vapor injections Celsius
Bacterium 35 minutes；
3rd, the truffle bacterium active peptide liquid of above-mentioned preparation is filtered into obtain pine below the dalton of molecular weight 5000 by ultrafiltration membrane filter
Reveal bacterium active peptide liquid；
4th, the truffle bacterium active peptide liquid of above-mentioned preparation is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the bottled freeze-dried powder product of truffle bacterium active peptide of the present invention；Pure water is added to press the truffle bacterium active peptide liquid of above-mentioned preparation
Oral liquid process specification is filling to oral liquid bottle, produces truffle bacterium oral liquid of active peptide product of the present invention；By above-mentioned preparation
Truffle bacterium active peptide liquid it is freeze-dried or spray drying be prepared into powdery granule, by capsule manufacturing techniques specification it is canned into
Capsule, produce truffle bacterium active peptide capsule product of the present invention.
Embodiment three, 1, the preparation of truffle bacterium active peptide liquid：Fresh or dry net truffle bacterium 20kg is chosen to carry out at crushing
Reason；The water of 12 times of addition crushing truffle bacterium weight is diluted immersion and stirred for 30 minutes, crushes the truffle bacterium after homogenate and adjusts
PH value 7.5, add cellulase, edible mushroom enzyme, neutral proteinase, papain（1:1:1:2）Multienzyme is digested, and enzyme is used
Measure and add 6500 enzyme-activity units for every gram of truffle bacterium（u）, 52 degrees Celsius of temperature control, digest 6 hours；Enzymolysis finishes boiling water heat
Centrifugation, centrifuge speed 10000rpm or so are cooled down after bathing enzyme deactivation 10 minutes, produces truffle bacterium active peptide liquid；
2nd, the truffle bacterium active peptide liquid of above-mentioned preparation is put into stainless steel cask enter steam sterilization cabinet and gone out in 115 degree of vapor injections Celsius
Bacterium 30 minutes；
3rd, the truffle bacterium active peptide liquid of above-mentioned preparation is filtered into obtain pine below the dalton of molecular weight 5000 by ultrafiltration membrane filter
Reveal bacterium active peptide liquid；
4th, the truffle bacterium active peptide liquid of above-mentioned preparation is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the bottled freeze-dried powder product of truffle bacterium active peptide of the present invention；Pure water is added to press the truffle bacterium active peptide liquid of above-mentioned preparation
Oral liquid process specification is filling to oral liquid bottle, produces truffle bacterium oral liquid of active peptide product of the present invention；By above-mentioned preparation
Truffle bacterium active peptide liquid it is freeze-dried or spray drying be prepared into powdery granule, by capsule manufacturing techniques specification it is canned into
Capsule, produce truffle bacterium active peptide capsule product of the present invention.
Example IV, 1, the preparation of truffle bacterium active peptide liquid：Fresh or dry net truffle bacterium 25kg is chosen to carry out at crushing
Reason；The water of 11 times of addition crushing truffle bacterium weight is diluted immersion and stirred for 30 minutes, crushes the truffle bacterium after homogenate and adjusts
PH value 7.5, add cellulase, edible mushroom enzyme, neutral proteinase, papain（1:1:1:2）Multienzyme is digested, and enzyme is used
Measure and add 5500 enzyme-activity units for every gram of truffle bacterium（u）, 55 degrees Celsius of temperature control, digest 6.5 hours；Enzymolysis finishes boiling water
Heating bath enzyme deactivation cools down centrifugation, centrifuge speed 10000rpm or so after 10 minutes, produces truffle bacterium active peptide liquid；
2nd, the truffle bacterium active peptide liquid of above-mentioned preparation is put into stainless steel cask enter steam sterilization cabinet and gone out in 125 degree of vapor injections Celsius
Bacterium 15 minutes；
3rd, the truffle bacterium active peptide liquid of above-mentioned preparation is filtered into obtain pine below the dalton of molecular weight 5000 by ultrafiltration membrane filter
Reveal bacterium active peptide liquid；
4th, the truffle bacterium active peptide liquid of above-mentioned preparation is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the bottled freeze-dried powder product of truffle bacterium active peptide of the present invention；Pure water is added to press the truffle bacterium active peptide liquid of above-mentioned preparation
Oral liquid process specification is filling to oral liquid bottle, produces truffle bacterium oral liquid of active peptide product of the present invention；By above-mentioned preparation
Truffle bacterium active peptide liquid it is freeze-dried or spray drying be prepared into powdery granule, by capsule manufacturing techniques specification it is canned into
Capsule, produce truffle bacterium active peptide capsule product of the present invention.
A kind of truffle bacterium active peptide prepared by the present invention is by peptide liquid according to freeze dryer on the filling lyophilized bottle of lyophilized technique specification
It is freeze-dried into bottled freeze-dried powder product, the additive-free preservative of vacuum freeze drying, safe, long shelf-life；Add pure
Water purification is filling into oral liquid product according to oral liquid process specification, and color is natural, distinctive natural flavour mountaineous good mouthfeel；Will
Hybrid peptide liquid is spray-dried or freeze-drying is prepared into powdery granule, filling into capsule product by capsule manufacturing techniques specification,
Take easy to carry.Present invention composition takes the rigorous Chinese medicine preparation specification of tradition, with the advanced processing work of modern biotechnology
Skill is prepared, and its process scientific and precise is reasonable, strong operability, is especially suitable for large-scale production.
Claims (4)
1. a kind of preparation method of truffle bacterium active peptide, it is characterised in that by the following steps：
（1）, truffle bacterium active peptide preparation：Choose truffle bacterium that is fresh or drying and carry out pulverization process；Addition crushes truffle bacterium
The water of 5-15 times of weight is diluted immersion and stirred for 30 minutes, crushes the truffle bacterium after homogenate and adjusts pH value 6-8, adds cellulose
Enzyme, edible mushroom enzyme, neutral proteinase, papain（1:1:1:2）Multienzyme is digested, and enzyme dosage is that every gram of truffle bacterium is added
5000-10000 enzyme-activity unit（u）, between 50-60 degrees Celsius of temperature control, digest 3-6 hours；Enzymolysis finishes boiling water heating bath
Centrifugation, centrifuge speed 4000-10000rpm or so are cooled down after enzyme deactivation 5-20 minutes, produces truffle bacterium active peptide liquid；
（2）, truffle bacterium active peptide liquid prepared by above-mentioned steps (1) put stainless steel cask and enters steam sterilization cabinet in 100-120 Celsius
Steam high-voltage sterilizing 15-40 minutes between degree；
（3）, by above-mentioned steps (2) prepare truffle bacterium active peptide liquid filter to obtain the dalton of molecular weight 5000 by ultrafiltration membrane filter
Following truffle bacterium active peptide liquid；
（4）, by above-mentioned steps（3）The truffle bacterium active peptide liquid of preparation is filling true to freeze dryer on cillin bottle by lyophilized technique specification
Vacuum freecing-dry, produce the bottled freeze-dried powder product of truffle bacterium active peptide of the present invention；The truffle bacterium active peptide liquid of above-mentioned preparation is added
Pure water is filling to oral liquid bottle by oral liquid process specification, produces truffle bacterium oral liquid of active peptide product of the present invention；Will
The truffle bacterium active peptide liquid of above-mentioned preparation is freeze-dried or spray drying is prepared into powdery granule, is advised by capsule manufacturing techniques
Model is canned into capsule, produces truffle bacterium active peptide capsule product of the present invention.
2. a kind of truffle bacterium active peptide according to claim 1 and preparation method thereof, it is characterized in that：Described pine
It is filling to freeze dryer vacuum freeze drying on cillin bottle by lyophilized technique specification to reveal bacterium active peptide liquid, produces truffle bacterium of the present invention and lives
The property bottled freeze-dried powder product of peptide；Add pure water filling by oral liquid process specification the truffle bacterium active peptide liquid of above-mentioned preparation
To oral liquid bottle, truffle bacterium oral liquid of active peptide product of the present invention is produced；The truffle bacterium active peptide liquid of above-mentioned preparation is chilled
Dry or spray drying is prepared into powdery granule, it is canned into capsule by capsule manufacturing techniques specification, produce truffle bacterium of the present invention
Active peptide capsule product.
3. a kind of truffle bacterium active peptide according to claim (1) and preparation method thereof, it is characterized in that：Described
Four kinds of enzymes of cellulase, edible mushroom enzyme, neutral proteinase, papain of truffle bacterium are digested, it is 1 that it, which forms weight ratio,：1：
1：2.
4. according to a kind of preparation method of truffle bacterium active peptide of claim (1) present invention, it is characterised in that：Described pine
It is using truffle bacterium that is fresh or drying as raw material to reveal bacterium active peptide, through broken, homogenate, enzymolysis, is centrifuged, the truffle bacterium that sterilizing forms
Active peptide liquid.
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| CN201710869656.0A CN107475340A (en) | 2017-09-23 | 2017-09-23 | A kind of preparation method of truffle bacterium active peptide |
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| CN201710869656.0A CN107475340A (en) | 2017-09-23 | 2017-09-23 | A kind of preparation method of truffle bacterium active peptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111227105A (en) * | 2020-01-15 | 2020-06-05 | 北京中科凯而健康科技有限公司 | Anti-fatigue small molecule peptide powder production equipment |
| CN112022955A (en) * | 2020-09-15 | 2020-12-04 | 安徽三九药业有限公司 | Black truffle, cordyceps sinensis and rhizoma polygonati granules and preparation method thereof |
| CN113383956A (en) * | 2021-07-06 | 2021-09-14 | 北京千菌方菌物科学研究院 | Black truffle kidney tonifying formula and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891838A (en) * | 2014-03-18 | 2014-07-02 | 徐庭虎 | Perigord truffle henry steudnera tuber cake |
| CN106692943A (en) * | 2017-02-09 | 2017-05-24 | 赵德润 | Method for preparing aspongopus bioactive peptide |
| CN106947797A (en) * | 2017-03-05 | 2017-07-14 | 赵德润 | A kind of rape pollen active peptide and preparation method thereof |
-
2017
- 2017-09-23 CN CN201710869656.0A patent/CN107475340A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891838A (en) * | 2014-03-18 | 2014-07-02 | 徐庭虎 | Perigord truffle henry steudnera tuber cake |
| CN106692943A (en) * | 2017-02-09 | 2017-05-24 | 赵德润 | Method for preparing aspongopus bioactive peptide |
| CN106947797A (en) * | 2017-03-05 | 2017-07-14 | 赵德润 | A kind of rape pollen active peptide and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111227105A (en) * | 2020-01-15 | 2020-06-05 | 北京中科凯而健康科技有限公司 | Anti-fatigue small molecule peptide powder production equipment |
| CN112022955A (en) * | 2020-09-15 | 2020-12-04 | 安徽三九药业有限公司 | Black truffle, cordyceps sinensis and rhizoma polygonati granules and preparation method thereof |
| CN113383956A (en) * | 2021-07-06 | 2021-09-14 | 北京千菌方菌物科学研究院 | Black truffle kidney tonifying formula and preparation method thereof |
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Application publication date: 20171215 |