KR100959496B1 - Blood glucose dropping Mulberry leaf tea cultured with Phellinus Linteus mycelium - Google Patents

Blood glucose dropping Mulberry leaf tea cultured with Phellinus Linteus mycelium Download PDF

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KR100959496B1
KR100959496B1 KR1020080003762A KR20080003762A KR100959496B1 KR 100959496 B1 KR100959496 B1 KR 100959496B1 KR 1020080003762 A KR1020080003762 A KR 1020080003762A KR 20080003762 A KR20080003762 A KR 20080003762A KR 100959496 B1 KR100959496 B1 KR 100959496B1
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weight
hypoglycemic
functional
mushroom mycelium
leaf tea
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KR20090078013A (en
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김기호
김혜자
이기남
김석환
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김기호
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/06—Treating tea before extraction; Preparations produced thereby
    • A23F3/12—Rolling or shredding tea leaves
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/06—Treating tea before extraction; Preparations produced thereby
    • A23F3/14—Tea preparations, e.g. using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/40—Tea flavour; Tea oil; Flavouring of tea or tea extract

Abstract

본 발명에 의한 혈당강하 기능성 상황버섯 균사체 배양 상엽차는 혈당저하 기능 및 혈압강하 등의 유효성분이 들어 있는 상엽(뽕나무 잎), 상지(뽕나무 가지), 상실(뽕나무 열매, 오디) 등과 홍삼, 산약, 천화분 등 한약재를 첨가하고 상황버섯(Phellinus Linteus) 균사체를 고체배양시켜 혼합함으로서 혈당강하, 콜레스테롤 저하 효과가 극대화된 기능성 상엽차에 관한 것이다.Hypoglycemic functional situation mushroom mycelium cultured upper leaf tea according to the present invention is the upper leaf (mulberry leaves), upper limbs (mulberry branches), loss (mulberry fruit, mulberry) and red ginseng, mountain medicine, natural flower containing active ingredients such as hypoglycemic function and lowering blood pressure By adding a herbal medicine and the culture of the Phellinus Linteus mycelium solid cultures and mixed with the hypoglycemic effect, cholesterol lowering effect is maximized functional functional tea.

본 발명에 의한 혈당강하 기능성 상황버섯 균사체 배양 상엽차는 상목(뽕나무)의 열매, 잎, 줄기, 뿌리 등에 함유되어 있는 DNJ(Dioxynojirimicin), 루틴, 식이섬유소, 칼슘, 철분, 아미노산 등의 유용약리 성분이 함유되어 있으며 홍삼, 산약, 천화분 등 한약재가 첨가되어 있고 특히 상황버섯 균사체가 배양되어 있어 베타글루칸 등에 의해 혈당강하 효과 및 콜레스테롤 저하 기능이 제고된 새로운 건강 기능성 상엽차이다.Hypoglycemic functional situation mushroom mycelium cultured leaf tea according to the present invention is useful pharmacological components such as DNJ (Dioxynojirimicin), rutin, dietary fiber, calcium, iron, amino acids contained in fruits, leaves, stems, roots, etc. It is a new health functional leaf tea with red ginseng, mountain medicinal herbs, cheonhwamin and other herbal medicines. Especially, mushroom mycelium is cultivated to improve blood sugar lowering effect and cholesterol lowering function by beta glucan.

본 발명에 의한 혈당강하 기능성 상황버섯 균사체 배양 상엽차는 맛, 향, 색 등 기호성과 혈당강하 효과, 콜레스테롤 저하 등의 기능성이 뛰어나 건강 기능성 차류(분말, 액상) 및 각종 건강 기능성 식품, 기능성 화장품, 의약품 등의 원료로 활용할 수 있게 된다.Hypoglycemic functional situation mushroom mycelium cultured green tea according to the present invention is excellent in taste, flavor, color, palatability, hypoglycemic effect, cholesterol lowering function, and health functional teas (powder, liquid) and various health functional foods, functional cosmetics, medicines It can be utilized as a raw material such as.

상엽, 상지, 상실, 혈당강하, 콜레스테롤 저하, 상황버섯, 베타글루칸  Upper lobe, upper limb, loss, hypoglycemic, cholesterol lowering, situation mushroom, beta glucan

Description

혈당강하 기능성 상황버섯 균사체 배양 상엽차{Blood glucose dropping Mulberry leaf tea cultured with Phellinus Linteus mycelium}Blood glucose dropping functional situation mushroom mycelium cultured tea leaf

본 발명은 상목(뽕나무)의 잎, 열매, 줄기, 뿌리를 세척, 살청, 유념, 건조하는 기술, 상목(잎, 열매, 줄기, 뿌리 등)에 홍삼 등 유용 한약재를 첨가하여 상황버섯 균사체를 배양시키는 기술, 상기 상황버섯 균사체 배양 상엽차를 살균, 건조, 분쇄, 포장하는 기술로 구성되어 있다.The present invention is to cultivate the situation mushroom mycelium by adding a useful herbal medicine such as red ginseng to the leaves, berries, stems, roots, washing techniques, apricots, remarks, drying technology of the common tree (Mulberry) It consists of a technique for sterilizing, drying, pulverizing, and packaging the mushroom mycelium cultured upper leaf tea.

본 발명에서는 혈당강하 기능성 복분자차를 제조함에 있어 혈당강하 기능 및 콜레스테롤 저하 효과가 높은 상목의 잎, 열매, 줄기, 뿌리 등 상목의 식물 전체를 활용하여 각종 유용 약리성분을 최대로 활용할 수 있고 홍삼 등 한약재를 첨가시켜 상황버섯 균사체의 배지로 활용, 상황버섯 균사체를 배양함으로서 약리성이 극대화된 새로운 혈당강하 기능성 상엽차로 개발할 수 있는 기술에 관한 것이다.In the present invention, in the preparation of hypoglycemic functional bokbunja tea can take full advantage of various useful pharmacological components such as leaves, berries, stems, roots of the common tree having a high hypoglycemic function and cholesterol-lowering effect and maximize the use of various useful pharmacological components, such as red ginseng The present invention relates to a technology that can be developed as a new hypoglycemic functional leaf tea with maximum pharmacological properties by adding herbal medicine as a medium for mushroom mushroom mycelium and cultivating mushroom mushroom mycelium.

본 발명은 상목의 잎, 열매, 줄기, 뿌리 등 유용부위의 맛, 향, 색 등을 개선하기 위하여 120℃∼180℃에서 살청(덖음)하여 기호성을 향상시키는 기술, 보존성 개선을 위하여 70℃∼150℃에서 살청 및 건조하는 기술, 열수에 의한 용출성 개선을 위하여 세포조직을 파쇄하는 유념기술 등을 제조에 관한 물리적인 배경기술로 확립하고 상황버섯 균사체를 배양시키기 위하여 상실(오디)와 홍삼, 산약, 천화분 등 한방제재를 혼합한 배지를 제조하여 살균, 균사체 접종, 15℃∼35℃에서의 균사체 배양 등의 미생물학적 배양기술을 개발하여 본 발명의 기본적 기술로 확립하였다.The present invention is a technique for improving palatability by improving the taste, flavor, color, etc. of useful parts such as leaves, berries, stems, roots of the common tree at 120 ℃ to 180 ℃, 70 ℃ to improve the preservation In order to establish the physical background technology for manufacturing the technology of desalination and drying at 150 ℃ and the crushing of cellular tissues to improve the elution by hot water, and to culture the situation mushroom mycelium, A medium containing herbal medicines, such as powdered herbs and natural powders, was prepared, and microbiological culture techniques such as sterilization, inoculation of mycelium, and mycelium culture at 15 ° C. to 35 ° C. were developed and established as basic techniques of the present invention.

상목은 열매, 잎, 줄기, 뿌리 등으로 구성되어 있으며 각각 부위별로 함유된 유용성분이나 약리효과 및 맛, 향, 색 등에서 다소간 차이가 있으나 상실, 상엽의 혈당강하 성분 및 기호성이 주로 식용 약용으로 활용되고 있다. 본 발명에서는 유용 약리성분이 다량 함유되어 있으나 기호성 및 가공방법 등이 개발되지 않아 활용도가 떨어지는 잎, 줄기, 뿌리 등을 활용 홍삼 등의 한약제재와 혼합하여 균사체 배양배지로 이용하는 방법, 베타글루칸 성분에 의한 콜레스테롤 저하, 면역 등의 효과가 뛰어난 것으로 입증된 상황버섯 균사체를 상목 한약 혼합배지에서 배양하여 상황버섯 균사체에 의한 혈당강하, 콜레스테롤 저하 효과의 극대화 방법, 상실의 발효숙성 시에 활용하는 50％ 정도의 설탕 등을 사용하지 않아 혈당강하 등의 기능성이 있으며 기호성과 보존성이 현저히 증가되는 가공방법에 관한 기술을 개발하고자 한다.It is composed of fruits, leaves, stems, roots, etc., but there are some differences in useful ingredients, pharmacological effects, tastes, aromas, colors, etc. contained in each part, but loss, upper blood sugar lowering component and palatability are mainly used for edible medicinal purposes. It is becoming. In the present invention, a useful pharmacological component is contained in a large amount, but since palatability and processing methods are not developed, the method is used as a mycelial culture medium by mixing with herbs, such as red ginseng, which has low utilization, such as leaves, stems, and roots. Strain mushroom mycelium, which has been proven to be effective in lowering cholesterol and immunity, is cultured in a mixed herbal medicine medium to maximize blood sugar lowering, cholesterol lowering effect, and 50% utilization of fermentation aging. It does not use sugar, etc., and it is to develop technology about processing method that has functionalities such as blood sugar drop and markedly increases palatability and preservation.

본 발명에 의한 혈당강하 기능성 상황버섯 균사체 배양, 상엽차는 상목의 열매를 중심으로 하여 잎, 줄기, 뿌리 등 모든 유용성분을 활용할 수 있으며 홍삼, 산약, 천화분 등 한약재의 유용성분, 상황버섯 균사체의 콜레스테롤 저하 기능 및 면역 효과 등에 의해 생리활성 기능성이 극대화된 새로운 상엽차를 제공해 줄 수 있는 효과가 있다. 또한 기존의 상엽차의 기호성분인 당성분에 의한 과도한 단맛을 개선할 수 있으며 별도의 당성분 첨가 없이 보존성을 향상시킬 수 있어 건강 기능성 차 용도 이외에 건강 기능성 식품(음료, 주류, 장류, 조미료, 면류, 과자류 등), 건강 기능성 화장품(미백, 항산화 등), 의약품(혈당강하제, 콜레스테롤 저하제, 혈압강하제 등)의 원료로의 활용효과도 기대할 수 있게 된다.Hypoglycemic functional situation mushroom mycelium culture, upper leaf tea according to the present invention can utilize all the useful ingredients such as leaves, stems, roots, etc. centering on the fruit of the common tree There is an effect that can provide a new leaf tea with the maximum physiological activity by the lowering function and immune effect. In addition, it is possible to improve excessive sweetness by sugar component, which is a favorite ingredient of conventional green tea, and to improve the preservation without adding sugar component, so that it can be used for health functional foods (drinks, liquor, soy sauce, seasonings, noodles) , Sweets, etc.), health functional cosmetics (whitening, antioxidants, etc.), pharmaceuticals (hypoglycemic agents, cholesterol lowering agents, blood pressure lowering agents, etc.) can be expected to be used as raw materials.

본 발명에 의한 혈당강하 기능성 상황버섯 균사체 배양 상엽차의 제조방법은 상엽(열매, 잎, 줄기, 뿌리)를 채취하여 정선, 세척, 살청, 유념, 건조하는 단계와 홍삼, 산약, 천화분 등 한약재를 첨가하는 단계, 상황버섯 균사체 종균을 접종하여 배양하는 단계, 상황버섯 균사체가 배양된 홍삼 등 한약재 함유 상엽차를 살균, 건조, 분쇄, 포장하는 단계로 구성되어 있다.The method for producing hypoglycemic-functional mushroom mycelium cultured upper leaf tea according to the present invention comprises extracting the upper leaves (fruit, leaves, stems, roots), and selecting herbs, washing, apricots, remarks, and drying herbs and herbal medicines such as red ginseng, acid powder, and natural flower. It consists of the step of adding, inoculating and incubating spawn mushroom mycelium spawn, sterilizing, drying, grinding, and packaging the herbal leaf tea containing red ginseng cultured with the situation mushroom mycelium.

본 발명의 구성에 관한 제조방법을 실시 예를 통하여 설명하면 다음과 같다.Referring to the manufacturing method according to the configuration of the present invention through the following examples.

1) 상황버섯 균사체를 PDB 배지에서 20℃∼35℃에서 3일∼7일간 진탕 암배양한다.1) Situary mushroom mycelium is shaken in culture PDB medium at 20-35 ℃ for 3-7 days.

2) 상실 10중량％∼50중량％, 상엽 10중량％∼50중량％, 상지 5중량％∼10중량％, 상백피 1중량％∼5중량％를 혼합한 후 홍삼 1중량％∼10중량％, 산약 1중량％∼10중량％, 천화분 1중량％∼10중량％를 첨가하여 50메쉬∼100메쉬로 분쇄한다.2) 1% by weight to 10% by weight of red ginseng, after mixing 10% by weight to 50% by weight, 10% by weight to 50% by weight of upper leaves, 5% to 10% by weight of upper limbs, and 1% to 5% by weight of lettuce; About 1% by weight to 10% by weight of acid, 1% by weight to 10% by weight of crushed powder is added, and pulverized into 50 mesh to 100 mesh.

3) 상기 2)의 상목, 한약재 혼합물을 120℃∼180℃의 팬에서 살청 후 유념시킨다.3) The mixture of medicinal herb and medicinal herb of 2) is agar after being soaked in a pan of 120 ° C to 180 ° C.

4) 상기 3)의 살청 및 유념된 상목 한약재 혼합물에 10중량％∼50중량％의 증류수를 넣고 살균하여 상기 1)의 상황버섯 균사체 배양액을 1중량％∼20중량％ 접종한다.4) 10 wt% to 50 wt% of distilled water is sterilized in the apricot and mixed medicinal herb mixture of 3) to inoculate 1 to 20 wt% of the mushroom mushroom mycelium culture medium of 1) above.

5) 상기 4)를 5일∼12일간 20℃∼35℃에서 진탕 암배양한다.5) Shake 4) is carried out at 20 ° C to 35 ° C for 5 to 12 days.

6) 상기 5)를 90℃의 건조기에서 30분∼60분 살균 후 70℃에서 24시간∼48시간 수분함량 7％ 이내가 되도록 건조한다.6) The above 5) is sterilized in a dryer at 90 ° C. for 30 minutes to 60 minutes and then dried at 70 ° C. so that the moisture content is within 7% for 24 hours to 48 hours.

7) 상기 6)을 100메쉬∼200메쉬로 분쇄하여 정선, 정량, 포장하여 완제품을 얻는다.7) Grind 6) above 100-200 mesh to select, quantify and package to obtain finished product.

[실험예][Experimental Example]

본 발명에 의한 혈당강하 기능성 상황버섯 균사체 배양 상엽차를 20배수의 정제수를 가하여 100℃에서 8시간 열수추출 후 원심분리한 다음 상등액을 감압농축 동결건조하여 혈당강하 기능과 관련하여 α-glucosidase 저해활성 실험을 실시한 결과 65％ 이상의 α-glucosidase 저해활성 효과가 있는 것으로 나타났다.Hypoglycemic functional status mushroom mycelium cultured supernatant according to the present invention was added to 20 times the purified water, and centrifuged after hot water extraction at 100 ° C for 8 hours, and then the supernatant was concentrated under reduced pressure to freeze the α-glucosidase activity in relation to the hypoglycemic function. Experimental results showed that the α-glucosidase inhibitory activity more than 65%.

Figure 112008500210741-pat00001
Figure 112008500210741-pat00001

본 발명에서의 혈당강하 기능성 상황버섯 균사체 배양 상엽차는는 상목의 열매, 잎, 줄기, 뿌리 등의 유용성분과 기호성, 홍삼, 산약, 천화분의 유용성과 기호성, 상황버섯 균사체에 의한 베타글루칸 등의 생리활성 성분이 함유되어 있어 혈당강하 기능 및 콜레스테롤 저하, 심혈관계의 질병 예방 및 치료 효과가 있으며 맛, 향, 색이 뛰어나고 기호성, 편의성 등이 개선된 차류(분말, 액상)을 제공해 줄 수 있다. 또한 상목의 잎, 줄기, 뿌리 등을 유용하게 활용할 수 있으며 혈당강하 효과, 면역 등 생리활성 기능이 있어 각종 기능성 식품, 기능성 화장품, 의약품 등의 원료로 활용할 수 있게 된다.Hypoglycemic-functional functional mushroom mycelium culture in the present invention is a useful component such as fruit, leaves, stems, roots and the like in the fruit of the common tree, the taste and taste of red ginseng, fungus, natural flower powder, bioactive components such as beta glucan by the situation mushroom mycelium It contains hypoglycemic function and cholesterol lowering, prevents and treats diseases of the cardiovascular system, and can provide tea (powder, liquid) with excellent taste, flavor, color, and improved taste and convenience. In addition, the leaves, stems, roots, etc. of the common tree can be usefully used, and have a physiological activity such as a hypoglycemic effect and immunity, and thus can be used as raw materials for various functional foods, functional cosmetics, and medicines.

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Claims (1)

[청구항 1] [Claim 1] 혈당강하 기능성 상황버섯 균사체 배양 상엽차의 제조방법에 있어서In the manufacturing method of hypoglycemic functional situation mushroom mycelium cultured green tea 1) PDB배지(Potato Dextrose Broth)에 상황버섯 균사체 종균을 PDB배지 총 중량에 대하여 1중량%~20중량% 접종하여 3일~7일간 20℃~35℃에서 진탕 암배양하여 전배양액을 만드는 단계.1) inoculating 1 to 20 wt% of the mushroom mushroom mycelium spawn in PDB medium (Potato Dextrose Broth) with respect to the total weight of PDB medium and shaking the cultures at 20 ℃ ~ 35 ℃ for 3 days to 7 days to make preculture . 2) 상실 10중량％∼50중량％, 상엽 10중량％∼50중량％, 상지 5중량％∼10중량％, 상백피 1중량％∼5중량％를 혼합한 후 홍삼 1중량％∼10중량％, 산약 1중량％∼10중량％, 천화분 1중량％∼10중량％를 첨가하여 50메쉬∼100메쉬로 분쇄하는단계2) 1% by weight to 10% by weight of red ginseng, after mixing 10% by weight to 50% by weight, 10% by weight to 50% by weight of upper leaves, 5% to 10% by weight of upper limbs, and 1% to 5% by weight of lettuce; Adding about 1% by weight to 10% by weight of acid, 1% by weight to 10% by weight of crushed powder, and then grinding into 50 mesh to 100 mesh 3) 상기 2)를 120℃∼180℃의 팬에서 살청 후 유념시키는 단계3) the step 2) after the desalination in a pan of 120 ℃ to 180 ℃ 4) 상기 3)에 10중량%~50중량%의 증류수를 넣고 그 총량에 대하여 상기 1)을 1중량%~20중량% 접종하는 단계.4) 10% to 50% by weight of distilled water in 3) step 1) to 1 to 20% by weight inoculated with respect to the total amount. 5) 상기 4)를 5일∼12일간 20℃∼35℃에서 진탕 암배양하는 단계5) the step of shaking cancer culture 4) at 20 ℃ to 35 ℃ for 5 to 12 days 6) 상기 5)를 건조기에 넣고 90℃에서 30분∼60분간 살균 후 70℃에서 24시간∼48시간 수분함량 7％ 이내로 건조하는 단계6) Put the above 5) in a drier and sterilization at 90 ℃ for 30 minutes to 60 minutes and then drying at 70 ℃ 24 hours to 48 hours within 7% moisture content 7) 상기 6)을 100메쉬∼200메쉬로 분쇄하여 정선, 정량, 포장하는 단계7) grinding the 6) to 100 mesh ~ 200 mesh to select, quantify, packing 8) 상기 1)~7)의 제조공정을 특징으로 하여 만들어지는 혈당 강하 기능성 상엽차의 제조방법.8) A method for producing a hypoglycemic functional upper leaf tea, characterized in that the manufacturing process of 1) to 7).
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