CN105062765A - Method for making cordyceps sinensis rice wine by means of continuously fermenting pure bacteria - Google Patents
Method for making cordyceps sinensis rice wine by means of continuously fermenting pure bacteria Download PDFInfo
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Abstract
The invention relates to a method for making cordyceps sinensis rice wine by means of continuously fermenting pure bacteria. The method includes washing rice by running water, draining the rice, uniformly stirring the rice in distilled water, canning and sealing the rice and sterilizing the rice; cooling the rice until the temperature of the rice is 20-25 DEG C for standby application; cultivating cordyceps militaris micro-particle strains with the strain preservation number of NKHB CM209635, inoculating and fermenting the cordyceps militaris micro-particle liquid strains in rice tanks, cultivating the cordyceps militaris micro-particle liquid strains under light-tight conditions for 5-10d, fermenting the cordyceps militaris micro-particle liquid strains, cultivating the cordyceps militaris micro-particle liquid strains under light conditions for 3-7d, sterilizing the cordyceps militaris micro-particle liquid strains and adding sterile water into the cordyceps militaris micro-particle liquid strains; inoculating rhizopus oryzae liquid micro-particle strains diluted by sterile water and cultivating the rhizopus oryzae liquid micro-particle strains for 36-48h; inoculating brewer's yeast strains diluted by sterile water, cultivating the brewer's yeast strains for 3-7d, and detecting total acid, total sugar and alcohol contents of the brewer's yeast strains at intervals of 12h; separately packaging the cordyceps sinensis rice wine; carrying out irradiation sterilization on the cordyceps sinensis rice wine at normal temperatures by the aid of <60>Co-gamma irradiation sources. The method has the advantages that the cordyceps militaris micro-particle liquid strains, the rhizopus oryzae liquid micro-particle strains and the brewer's yeast strains are continuously fermented under specific conditions, accordingly, the method is low in cost, and the obtained cordyceps sinensis rice wine contains abundant active components and nutrition.
Description
Technical field
The present invention relates to a kind of simple bacterium fermentation continuously for the method for Chinese caterpillar fungus rice wine.
Background technology
Cordyceps militaris (L.) Link. (Cordycepsmilitaris), belongs to Ascomycotina, ergot Zoopagales, the type species of Clavicipitaceae, Cordyceps.Cordyceps militaris (L.) Link. contains various active material, comprises Cordyceps polysaccharide, cordycepin, cordycepic acid, adenosine, nucleosides, ergosterol, SOD etc.
Rice wine, also known as fermented glutinour rice, sweet wine, is China's a kind of traditional fermented food among the people, and the edible history of existing two thousand years, ethanol content is low, taste is fragrant and sweet pure and sweet, deeply likes by the numerous common people.Rice wine take glutinous rice as raw material, makes with the fermentation of self-control natural distiller's yeast, and containing the abundant nutritive substance such as reducing sugar, amino acid, microorganism, low alcohol, has clearing and activating the channels and collaterals, tonifying Qi of nourishing blood, nourishing Yin and moistening lung effect.It is simple that Conventional processing methods makes rice wine process, with glutinous rice, rice for raw material, ferments with koji.Koji belongs to mixed starters, containing a large amount of yeast, outside a small amount of rhizopus, also has a large amount of miscellaneous bacteria, so distiller's yeast quality is very unstable, the rice wine thick taste of working it out and sweet taste is not enough, or alcoholic strength is very low, even make rice wine failure, the rice wine mouthfeel being difficult to accomplish to produce is remained the same from beginning to end.
Existing rice wine and making method, as the preparation method of the Chinese caterpillar fungus rice wine of a kind of Anshen Bunao of CN102477394 announcement, utilize Cordyceps sinensis 10 grams, longan pulp 30 grams, ginseng 20 grams, 30 grams, date, structure to offer sacriffices to the gods or the spirits of the dead 30 grams, by rice wine lixiviate.A kind of health-care rice wine that CN103382427 announces, its making method is Secondary Fermentation, first ferment after the mixing of millet, koji for uncooked material and underground deep layer water, carry out again distilling out white wine after fermentation, again using white wine as wine base, millet rice carries out boiling after soaking with date, add Daqu after boiling again to ferment, squeeze after having fermented, add rock sugar, honey and Chinese medicine auxiliary material after squeezing to carry out blending toning, then kill virus after filtering, through filling and sealing after the assay was approved, be finally prepared into finished rice wine packaging warehouse-in.
Summary of the invention
The object of the invention is for the above-mentioned state of the art, aim to provide a kind of activeconstituents, nutritious; Preparation method is pollution-free, noresidue, operation is continuous, cost is low, efficiency is high continuously simple bacterium fermentation for the method for Chinese caterpillar fungus rice wine.
The implementation of the object of the invention is, simple bacterium fermentation is for the method for Chinese caterpillar fungus rice wine continuously, and concrete steps are as follows:
(1) screen rice, flowing water stirring and washing 40 ~ 90s, drains;
(2) adding distil water in 1-1.5kg rice, the ratio of water and rice is 1.2 ~ 3:1, stirs evenly;
(3) by rice tinning, sealing, in 121 DEG C, 1.034 × 10
5pa, sterilizing 15 ~ 30min; Be cooled to 20 ~ 25 DEG C again, for subsequent use;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: ground rice 100 ~ 200g, sucrose 20 ~ 40g, yeast powder 6 ~ 10g, MgSO
4/ MgCl
23 ~ 6, KH
2pO
4/ K
2hPO
43 ~ 4g, adds sterile distilled water to 1000ml; In temperature 20 ~ 23 DEG C, pH6 ~ 7, cultivate 5 ~ 6d under shearing rotating speed 800 ~ 1200rpm condition, obtain the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635, for subsequent use;
(5) in the glutinous rice tank of step (3), glutinous rice quality 1 ~ 10% is accessed, the Cordyceps militaris (L.) Link. microparticle liquid spawn that step (4) is cultivated; 20 ~ 25 DEG C of fermentations, lucifuge cultivates 5 ~ 10d, stirs once every 12h;
(6) 20 ~ 25 DEG C of fermentations, see that light cultivates 3 ~ 7d, light intensity is 100 ~ 1200Lux, stirs once every 12h;
(7) fermentation is stopped, 115 DEG C, 0.676 × 10
4pa, sterilizing 15min; Add the sterilized water of its quality 10 ~ 30%;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: ground rice 100 ~ 200g, potato leaching powder 20 ~ 60g, sucrose 20 ~ 40g, MgSO4/MgC
l20.5 ~ 2g, KH
2pO
4/ K
2hPO
41 ~ 4g, adds water to 1000ml; In temperature 28 DEG C, pH6 ~ 7, shear rotating speed 800 ~ 1200rpm, cultivate 36 ~ 48h, obtain the Rhizopus oryzae liquid microparticle bacterial classification that bacterial strain deposit number is CCTCCAF93150, for subsequent use;
Wherein KH
2pO
4/ K
2hPO
4=1:1;
(9) get 1 ~ 10%% of screening meter Zhi Liang in step (1), the Rhizopus oryzae liquid microparticle bacterial classification that step (8) is cultivated, adds sterilized water and is diluted to 3 ~ 5 times, in the tank of access step (8), cultivates 36 ~ 48h for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: ground rice 100 ~ 200g, casein peptone 3 ~ 8g, Fructus Hordei Germinatus leaching powder 3 ~ 10g, sucrose 10 ~ 40g, yeast leaching powder 3 ~ 6g, adds water to 1000ml; Culture condition: temperature 28 DEG C, pH6 ~ 7, shear rotating speed 800 ~ 1200rpm, and cultivate 32 ~ 48h, obtaining bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification, for subsequent use;
(11) 1 ~ 5% of screening meter Zhi Liang in step (1) is got, the yeast saccharomyces cerevisiae bacterial classification that step (10) is cultivated, adds sterilized water and is diluted to 3 ~ 5 times, in the tank of access step (9), cultivate 3 ~ 7d for 28 DEG C, detect total acid, total reducing sugar, alcoholic strength every 12h;
(12) fermentation is stopped, packing, packaging;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 1 ~ 10kGy.
In traditional food, quote Cordyceps militaris (L.) Link. fungus, the means transformed by biological fermentation introduce nutrition and active substance in rice wine food, make rice wine food innovate upgrading.
The present invention adopts Cordyceps militaris spawn, Rhizopus oryzae bacterial classification, yeast saccharomyces cerevisiae bacterial classification to continuously ferment under each single-minded condition, three kinds of simple bacterial strains continuously ferment to transform and introduce physiologically active substance in rice wine, as cordycepin, Cordyceps polysaccharide, adenosine etc., cost is low, and gained Chinese caterpillar fungus rice wine is containing thing activeconstituents, nutritious; Preparation method is pollution-free, noresidue, green health, operation is continuously, cost is low, efficiency is high.
Accompanying drawing explanation
Fig. 1 is Cordyceps militaris spawn figure,
Fig. 2 is Chinese caterpillar fungus rice wine, common sticky rice wine, general brown rice sweet wine figure,
Fig. 3 is adenosine reference substance HPLC color atlas,
Fig. 4 is Chinese caterpillar fungus rice wine adenosine HPLC chromatogram.
Embodiment
Adopt the present invention, screening rice, flowing water cleans, and drain, adding distil water, the ratio of water and rice is 1 ~ 3:1, stirs evenly, and tinning seals, sterilizing.Preferably, in step (2), the ratio of water and rice is: 1.5 ~ 2:1.To screen rice be glutinous rice, brown rice or crack rice.
Be cooled to 20 ~ 25 DEG C, for subsequent use; Cultivate the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635.Cordyceps militaris spawn substratum: ground rice 100 ~ 200g, sucrose 20 ~ 40g, yeast powder 6 ~ 10g, MgSO
4/ MgCl
23 ~ 6g, KH
2pO
4/ K
2hPO
43 ~ 4g, adds sterile distilled water to 1000ml; In temperature 20 ~ 23 DEG C, pH6 ~ 7, cultivate 5 ~ 6d under shearing rotating speed 800 ~ 1200rpm condition, obtain the Cordyceps militaris (L.) Link. microparticle bacterial classification that the bacterial strain deposit number shown in Fig. 1 is NKHBCM209635.Thalli granule particle diameter is 0.2 ~ 0.7mm, and cell concentration is 10
3~ 10
5individual/ml.
Preferably, Cordyceps militaris spawn substratum: ground rice 150 ~ 200g, sucrose 20 ~ 30g, yeast powder 6 ~ 8g, MgSO
4/ MgCl
25g, KH
2pO
4/ K
2hPO
44g, adds sterile distilled water to 1000ml; In temperature 22 DEG C, pH7, cultivates 6d under shearing rotating speed 1000rpm condition.Cordyceps militaris (L.) Link. thalline grain diameter is 0.3 ~ 0.5mm, and cell concentration is 5 × 10
3~ 10
4individual/ml.
In step (5), (6), access 1 ~ 10% Cordyceps militaris (L.) Link. microparticle liquid fungus seed in glutinous rice tank, lucifuge cultivates 5 ~ 10d, fermentation, and see that light cultivates 3 ~ 7d, light intensity is 100 ~ 1200Lux.Preferably, access 2 ~ 3% Cordyceps militaris (L.) Link. microparticle liquid fungus seed in glutinous rice tank, lucifuge cultivates 5 ~ 7d, fermentation, and see that light cultivates 3 ~ 5d, light intensity is 200 ~ 500Lux.Sterilizing, adds sterilized water.
Cultivate the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150, Rhizopus oryzae bacterium culture medium: ground rice 100 ~ 200g, potato leaching powder 20 ~ 60g, sucrose 20 ~ 40g, MgSO4/MgC
l20.5 ~ 2g, KH
2pO
4/ K
2hPO
41 ~ 4g, adds water to 1000ml; In temperature 28 DEG C, pH6 ~ 7, shear rotating speed 800 ~ 1200rpm, cultivate 36 ~ 48h.Preferably, in step (8), Rhizopus oryzae bacterium culture medium: ground rice 140 ~ 160g, potato leaching powder 30 ~ 40g, sucrose 20 ~ 30g, MgSO4/MgC
l20.5 ~ 1g, KH
2pO
4/ K
2hPO
42 ~ 3g, adds water to 1000ml; In temperature 28 DEG C, pH6.5, shears rotating speed 1000rpm, cultivates 40h.
Step (8) obtained strains deposit number is the Rhizopus oryzae bacterial classification grain diameter of CCTCCAF93150 is 0.2 ~ 0.5mm, and cell concentration is 10
3~ 10
5individual/ml.
Get 1 ~ 10% Rhizopus oryzae microparticle liquid spawn of screening meter Zhi Liang in step (1), add sterilized water and be diluted to 3 ~ 5 times, in the tank of access step (8), cultivate 36 ~ 48h for 28 DEG C.Preferably, get 3 ~ 5% Rhizopus oryzae microparticle liquid spawns of screening meter Zhi Liang in step (1) in step (9), add sterilized water and be diluted to 5 times, in the tank of access step (8), cultivate 36 ~ 40h for 28 DEG C.
Rhizopus oryzae liquid-spawn inoculation amount is 3 ~ 5%, adds sterilized water and dilutes 5 times, and fermentation time is 36 ~ 40h.
Cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification, yeast saccharomyces cerevisiae bacterial classification substratum: ground rice 100 ~ 200g, casein peptone 3 ~ 8g, Fructus Hordei Germinatus leaching powder 3 ~ 10g, sucrose 10 ~ 40g, and yeast leaching powder 3 ~ 6g, adds water to 1000ml; Culture condition: temperature 28 DEG C, pH6 ~ 7, shear rotating speed 800 ~ 1200rpm, cultivate 32 ~ 48h.Preferably, in step (10), yeast saccharomyces cerevisiae bacterial classification substratum: ground rice 150 ~ 200g, casein peptone 4 ~ 6g, Fructus Hordei Germinatus leaching powder 3 ~ 5g, sucrose 10 ~ 20g, yeast leaching powder 4 ~ 5g, adds water to 1000ml; Culture condition: temperature 28 DEG C, pH6.5, shears rotating speed 800rpm, cultivates 32h.The yeast saccharomyces cerevisiae bacterial classification concentration that step (10) is cultivated is 5 × 10
6~ 10
7individual/ml.
In step (4), (8), (10), ground rice used is brown rice or powder of cracking rice.
Get the yeast saccharomyces cerevisiae bacterial classification of 1 ~ 5% of screening meter Zhi Liang in step (1), preferably, get the yeast saccharomyces cerevisiae bacterial classification of 2 ~ 3% of screening meter Zhi Liang in step (1).Cultivate 3 ~ 7d, detect total acid, total reducing sugar, alcoholic strength every 12h.Packing, packaging;
60co-γ irradiation source normal temperature irradiation sterilization obtains Chinese caterpillar fungus rice wine.The careless cellulose content 0.3 ~ 1.0 ‰ of gained Chinese caterpillar fungus rice wine worm, Cordyceps polysaccharide content 0.2 ~ 3.0%, adenosine content 0.5 ~ 2.0 ‰, ethanol content 1 ~ 10%, total acid content 1.0 ~ 10g/L, total sugar content 100g ~ 200g/L, reducing sugar content 5 ~ 10%, solid content 20 ~ 30%.
Adopt
60co-γ irradiation source normal temperature irradiation sterilization, does not destroy nutrition, activeconstituents, does not destroy outer packaging.
Common sticky rice wine, general brown rice sweet wine are shown in Fig. 2 with the rice wine Chinese caterpillar fungus rice wine figure adopting the present invention to prepare, and from Fig. 1,2, Chinese caterpillar fungus rice wine has showed color and luster and the form of Chinese caterpillar fungus in appearance, illustrates that Chinese caterpillar fungus rice wine has the attribute of Chinese caterpillar fungus.
Adenosine reference substance HPLC color atlas is shown in Fig. 3, and Chinese caterpillar fungus rice wine adenosine HPLC chromatogram is shown in Fig. 4, and from figure, 3,4 visible Chinese caterpillar fungus rice wine and adenosine standard substance have the object peak of identical retention time, illustrates in Chinese caterpillar fungus rice wine containing adenosine species activity composition.
With specific embodiment in detail the present invention is described in detail below:
Embodiment 1
(1) screen glutinous rice, flowing water stirring and washing 40 ~ 60s, namely drainage stops without turbidity, colourity, drains;
(2) add 1.5L distilled water in 1kg glutinous rice, stir evenly;
(3) by glutinous rice tinning, sealing, in 121 DEG C, 1.034 × 10
5pa, sterilizing 15min; Be cooled to 20 ~ 25 DEG C again, for subsequent use;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: coarse rice powder 150g, sucrose 30g, yeast powder 6g, MgSO
4/ MgCl
23g, KH
2pO
4/ K
2hPO
44g, adds sterile distilled water to 1000ml; In temperature 22 DEG C, pH6.5, cultivates 6d under shearing rotating speed 1000rpm condition, obtains the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635, for subsequent use; Wherein KH
2pO
4/ K
2hPO
4=1:1;
(5) in the glutinous rice tank of step (3), glutinous rice quality 5% is accessed, the Cordyceps militaris (L.) Link. microparticle liquid spawn that step (4) is cultivated; 20 DEG C of fermentations, lucifuge cultivates 7d, stirs once every 12h;
(6) 20 DEG C of fermentations, see that light cultivates 5d, light intensity is 1000Lux, stirs once every 12h;
(7) fermentation is stopped, 115 DEG C, 0.676 × 10
4pa, sterilizing 15min; Add the sterilized water of its quality 10%;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: coarse rice powder 100g, potato leaching powder 50g, sucrose 20g, MgSO4/MgC
l20.5g, KH
2pO
4/ K
2hPO
41g, adds water to 1000ml; In temperature 28 DEG C, pH6.5, shears rotating speed 1000rpm, cultivates 40h, obtains the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150, for subsequent use;
Wherein KH
2pO
4/ K
2hPO
4=1:1;
(9) get 5% of screening meter Zhi Liang in step (1), the Rhizopus oryzae liquid microparticle bacterial classification that step (8) is cultivated, adds sterilized water and is diluted to 5 times, in the tank of access step (8), cultivates 40h for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: coarse rice powder 100g, casein peptone 5g, Fructus Hordei Germinatus leaching powder 3g, sucrose 10g, yeast leaching powder 3g, adds water to 1000ml; Culture condition: temperature 28 DEG C, pH6.5, shears rotating speed 1000rpm, and cultivate 40h, obtaining bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification, for subsequent use;
(11) 5% of screening meter Zhi Liang in step (1) is got, the yeast saccharomyces cerevisiae bacterial classification that step (10) is cultivated, adds sterilized water and is diluted to 3 times, in the tank of access step (9), cultivate 5d for 28 DEG C, detect total acid, total reducing sugar, alcoholic strength every 12h;
(12) fermentation is stopped, packing, packaging;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 3kGy.
Embodiment 2, with embodiment 1, unlike,
(2) add 1.2L distilled water in 1kg brown rice, stir evenly;
(3) by brown rice tinning, sealing, in 121 DEG C, 1.034 × 10
5pa, sterilizing 25min; Be cooled to 20 ~ 25 DEG C again, for subsequent use;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: coarse rice powder 100g, sucrose 40g, yeast powder 8g, MgSO
4/ MgCl
25g, KH
2pO
4/ K
2hPO
44g, adds sterile distilled water to 1000ml; In temperature 20 DEG C, pH6.5, cultivates 6d under shearing rotating speed 1200rpm condition, for subsequent use;
(5) in brown rice tank, access the Cordyceps militaris (L.) Link. microparticle liquid spawn of brown rice quality 3%; 20 DEG C of fermentations, lucifuge cultivates 5d;
(6) 22 DEG C of fermentations, see that light cultivates 5d, light intensity is 500Lux, stirs once every 12h;
(7) stop fermentation, add the sterilized water of its quality 30%;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: coarse rice powder 150g, potato leaching powder 30g, sucrose 30g, MgSO4/MgC
l21g, KH
2pO
4/ K
2hPO
42g, adds water to 1000ml; In temperature 28 DEG C, pH6.0, shears rotating speed 1000rpm, cultivates 48h;
(9) get the Rhizopus oryzae liquid microparticle bacterial classification of 3% of screening meter Zhi Liang in step (1), add sterilized water and be diluted to 3 times, in the tank of access step (8), cultivate 48h for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: coarse rice powder 200g, casein peptone 6g, Fructus Hordei Germinatus leaching powder 7g, sucrose 20g, yeast leaching powder 5g, adds water to 1000ml; PH7, shears rotating speed 1000rpm, cultivates 36h;
(11) get the yeast saccharomyces cerevisiae bacterial classification of 3% of screening meter Zhi Liang in step (1), add sterilized water dilution cultivation 3 times;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 10kGy.
Embodiment 3, with embodiment 1, unlike,
(1) screening is cracked rice, and running water 50 ~ 70s, drains;
(2) 1kg adds 3L distilled water in cracking rice, and stirs evenly;
(3) will to crack rice tinning, sealing, in 121 DEG C, 1.034 × 10
5pa, sterilizing 20min;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: crack rice powder 200g, sucrose 20g, yeast powder 10g, MgSO
4/ MgCl
26g, KH
2pO
4/ K
2hPO
43g, adds sterile distilled water to 1000ml; In temperature 23 DEG C, pH6.0, cultivates 6d under shearing rotating speed 1000rpm condition;
(5) the Cordyceps militaris (L.) Link. microparticle liquid spawn of quality 10% of cracking rice in access in tank of cracking rice; 22 DEG C of fermentations, lucifuge cultivates 10d;
(6) 22 DEG C of fermentations, see that light cultivates 3d, light intensity is 200Lux;
(7) stop fermentation, add the sterilized water of its quality 20%;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: crack rice powder 100g, potato leaching powder 60g, sucrose 20g, MgSO4/MgC
l22g, KH
2pO
4/ K
2hPO
44g, adds water to 1000ml; In temperature 28 DEG C, pH7.0, shears rotating speed 800rpm, cultivates 36h;
(9) get the Rhizopus oryzae liquid microparticle bacterial classification of 1% of screening meter Zhi Liang in step (1), add sterilized water and be diluted to 3 times, in the tank of access step (8), cultivate 36h for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: crack rice powder 150g, casein peptone 3g, Fructus Hordei Germinatus leaching powder 10g, sucrose 40g, yeast leaching powder 6g, adds water to 1000ml; PH6.0, shears rotating speed 1000rpm, cultivates 48h;
(11) get the yeast saccharomyces cerevisiae bacterial classification of 5% of screening meter Zhi Liang in step (1), add sterilized water dilution cultivation 5 times, cultivate 3d for 28 DEG C;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 1kGy.
Embodiment 4, with embodiment 1, unlike,
(1) screen glutinous rice, running water 50 ~ 60s, drains;
(2) add 2L distilled water in 1.5kg glutinous rice, stir evenly;
(3) by glutinous rice tinning, sealing, in 121 DEG C, 1.034 × 10
5pa, sterilizing 20min;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: coarse rice powder 120g, sucrose 40g, yeast powder 7g, MgSO
4/ MgCl
24g, KH
2pO
4/ K
2hPO
44g, adds sterile distilled water to 1000ml; In temperature 23 DEG C, pH6.0, cultivates 6d under shearing rotating speed 1200rpm condition;
(5) in glutinous rice tank, access the Cordyceps militaris (L.) Link. microparticle liquid spawn of glutinous rice quality 1%; 25 DEG C of fermentations, lucifuge cultivates 10d;
(6) 25 DEG C of fermentations, see that light cultivates 6d, light intensity is 900Lux;
(7) stop fermentation, add the sterilized water of its quality 15%;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: coarse rice powder 100g, potato leaching powder 20g, sucrose 40g, MgSO4/MgC
l20.5g, KH
2pO
4/ K
2hPO
43g, adds water to 1000ml; In temperature 28 DEG C, pH6.5, shears rotating speed 800rpm, cultivates 36h;
(9) get the Rhizopus oryzae liquid microparticle bacterial classification of 10% of screening meter Zhi Liang in step (1), add sterilized water and be diluted to 3 times, in the tank of access step (8), cultivate 42h for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: coarse rice powder 200g, casein peptone 8g, Fructus Hordei Germinatus leaching powder 3g, sucrose 10g, yeast leaching powder 3g, adds water to 1000ml; In temperature 28 DEG C, pH6.5, shears rotating speed 1200rpm, cultivates 32h;
(11) get the yeast saccharomyces cerevisiae bacterial classification of 2% of screening meter Zhi Liang in step (1), add sterilized water dilution cultivation 5 times, cultivate 4d for 28 DEG C;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 5kGy.
Embodiment 5, with embodiment 1, unlike,
(1) screen brown rice, running water 60 ~ 90s, drains;
(2) add 2.5L distilled water in 1.5kg brown rice, stir evenly;
(3) by brown rice tinning, sealing, in 121 DEG C, 1.034 × 10
5pa, sterilizing 25min;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: coarse rice powder 160g, sucrose 20g, yeast powder 8g, MgSO
4/ MgCl
25g, KH
2pO
4/ K
2hPO
44g, adds sterile distilled water to 1000ml; In temperature 22 DEG C, pH6.0, cultivates 5d under shearing rotating speed 1200rpm condition;
(5) in brown rice tank, access the Cordyceps militaris (L.) Link. microparticle liquid spawn of glutinous rice quality 2%; 25 DEG C of fermentations, lucifuge cultivates 10d;
(6) 25 DEG C of fermentations, see that light cultivates 7d, light intensity is 100Lux;
(7) stop fermentation, add the sterilized water of its quality 20%;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: coarse rice powder 200g, potato leaching powder 40g, sucrose 40g, MgSO4/MgC
l21g, KH
2pO
4/ K
2hPO
44g, adds water to 1000ml; In temperature 28 DEG C, pH7.0, shears rotating speed 1200rpm, cultivates 46h;
(9) get the Rhizopus oryzae liquid microparticle bacterial classification of 7% of screening meter Zhi Liang in step (1), add sterilized water and be diluted to 5 times, in the brown rice tank of access step (8), cultivate 46h for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: coarse rice powder 160g, casein peptone 8g, Fructus Hordei Germinatus leaching powder 1g, sucrose 10g, yeast leaching powder 3g, adds water to 1000ml; Temperature 28 DEG C, pH6.5, shears rotating speed 900rpm, cultivates 42h;
(11) get the yeast saccharomyces cerevisiae bacterial classification of 4% of screening meter Zhi Liang in step (1), add sterilized water dilution cultivation 3 times, cultivate 7d for 28 DEG C;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 8kGy.
Embodiment 6, with embodiment 1, unlike,
(1) screening is cracked rice, and running water 60 ~ 90s, drains;
(2) 1.5kg adds 2L distilled water in cracking rice, and stirs evenly;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: crack rice powder 150g, sucrose 30g, yeast powder 10g, MgSO
4/ MgCl
26g, KH
2pO
4/ K
2hPO
44g, adds sterile distilled water to 1000ml; In temperature 20 DEG C, pH7.0, cultivates 5d under shearing rotating speed 1000rpm condition;
(5) in glutinous rice tank, access the Cordyceps militaris (L.) Link. microparticle liquid spawn of quality 8% of cracking rice; 25 DEG C of fermentations, lucifuge cultivates 5d;
(6) 21 DEG C of fermentations, see that light cultivates 3d, light intensity is 1200Lux;
(7) stop fermentation, add the sterilized water of its quality 30%;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: crack rice powder 150g, potato leaching powder 30g, sucrose 20g, MgSO4/MgC
l20.5g, KH
2pO
4/ K
2hPO
43g, adds water to 1000ml; In temperature 28 DEG C, pH7.0, shears and turns 1000rpm, cultivates 40h;
(9) get the Rhizopus oryzae liquid microparticle bacterial classification of 5% of screening meter Zhi Liang in step (1), add sterilized water and be diluted to 3 times, access step (8) crack rice in tank, cultivate 46h for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: crack rice powder 200g, casein peptone 3g, Fructus Hordei Germinatus leaching powder 5g, sucrose 30g, yeast leaching powder 6g, adds water to 1000ml; Temperature 28 DEG C, pH6.0, shears rotating speed 800rpm, cultivates 32h;
(11) get the yeast saccharomyces cerevisiae bacterial classification of 5% of screening meter Zhi Liang in step (1), add sterilized water dilution cultivation 3 times, access step (9) is cracked rice in tank, cultivates 6d for 28 DEG C;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 6kGy.
Claims (14)
1. simple bacterium fermentation, for the method for Chinese caterpillar fungus rice wine, is characterized in that: concrete steps are as follows continuously:
(1) screen rice, flowing water stirring and washing 40 ~ 90s, drains;
(2) adding distil water in 1-1.5kg rice, the ratio of water and rice is 1.2 ~ 3:1, stirs evenly;
(3) by rice tinning, sealing, in 121 DEG C, 1.034 × 10
5pa, sterilizing 15 ~ 30min; Be cooled to 20 ~ 25 DEG C again, for subsequent use;
(4) the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635 is cultivated,
Cordyceps militaris spawn substratum: ground rice 100 ~ 200g, sucrose 20 ~ 40g, yeast powder 6 ~ 10g, MgSO
4/ MgCl
23 ~ 6, KH
2pO
4/ K
2hPO
43 ~ 4g, adds sterile distilled water to 1000ml; In temperature 20 ~ 23 DEG C, pH6 ~ 7, cultivate 5 ~ 6d under shearing rotating speed 800 ~ 1200rpm condition, bacterial strain deposit number is the Cordyceps militaris (L.) Link. microparticle bacterial classification of NKHBCM209635, for subsequent use;
(5) in the rice tank of step (3), meter Zhi Liang 1 ~ 10% is accessed, the Cordyceps militaris (L.) Link. microparticle liquid spawn that step (4) is cultivated; 20 ~ 25 DEG C of fermentations, lucifuge cultivates 5 ~ 10d, stirs once every 12h;
(6) 20 ~ 25 DEG C of fermentations, see that light cultivates 3 ~ 7d, light intensity is 100 ~ 1200Lux, stirs once every 12h;
(7) fermentation is stopped, 115 DEG C, 0.676 × 10
4pa, sterilizing 15min; Add the sterilized water of its quality 10 ~ 30%, obtain the Cordyceps militaris (L.) Link. microparticle bacterial classification that bacterial strain deposit number is NKHBCM209635;
(8) the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 is cultivated,
Rhizopus oryzae bacterium culture medium: ground rice 100 ~ 200g, potato leaching powder 20 ~ 60g, sucrose 20 ~ 40g, MgSO4/MgC
l20.5 ~ 2g, KH
2pO
4/ K
2hPO
41 ~ 4g, adds water to 1000ml; In temperature 28 DEG C, pH6 ~ 7, shear rotating speed 800 ~ 1200rpm, cultivate 36 ~ 48h, obtain the Rhizopus oryzae liquid microparticle bacterial classification that bacterial strain deposit number is CCTCCAF93150, for subsequent use;
Wherein KH
2pO
4/ K
2hPO
4=1:1;
(9) 1 ~ 10%% of screening meter Zhi Liang in step (1) is got, the Rhizopus oryzae liquid microparticle bacterial classification that step (8) is cultivated, add sterilized water and be diluted to 3 ~ 5 times, in the tank of access step (8), cultivate 36 ~ 48h, obtain the Rhizopus oryzae bacterial classification that bacterial strain deposit number is CCTCCAF93150 for 28 DEG C;
(10) cultivating bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification,
Yeast saccharomyces cerevisiae bacterial classification substratum: ground rice 100 ~ 200g, casein peptone 3 ~ 8g, Fructus Hordei Germinatus leaching powder 3 ~ 10g, sucrose 10 ~ 40g, yeast leaching powder 3 ~ 6g, adds water to 1000ml; Culture condition: temperature 28 DEG C, pH6 ~ 7, shear rotating speed 800 ~ 1200rpm, and cultivate 32 ~ 48h, obtaining bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification, for subsequent use;
(11) 1 ~ 5% of screening meter Zhi Liang in step (1) is got, the yeast saccharomyces cerevisiae liquid spawn that step (10) is cultivated, add sterilized water and be diluted to 3 ~ 5 times, in the tank of access step (9), cultivate 3 ~ 7d for 28 DEG C, detect total acid, total reducing sugar, alcoholic strength every 12h;
(12) stop fermentation, obtaining bacterial strain deposit number is CCTCCAY93158 yeast saccharomyces cerevisiae bacterial classification, packing, packaging;
(13)
60co-γ irradiation source normal temperature irradiation sterilization, radiation absorber amount is 1 ~ 10kGy.
2. continuously simple bacterium fermentation according to claim 1, for the method for Chinese caterpillar fungus rice wine, is characterized in that: step (1) to screen rice be glutinous rice, brown rice or crack rice, the ratio of step (2) water and rice is: 1.5 ~ 2:1.
3. simple bacterium fermentation continuously according to claim 1, for the method for Chinese caterpillar fungus rice wine, is characterized in that: Cordyceps militaris spawn substratum in step (4): ground rice 150 ~ 200g, sucrose 20 ~ 30g, yeast powder 6 ~ 8g, MgSO
4/ MgCl
25g, KH
2pO
4/ K
2hPO
44g, adds sterile distilled water to 1000ml; In temperature 22 DEG C, pH7, cultivates 6d under shearing rotating speed 1000rpm condition.
4. simple bacterium fermentation continuously according to claim 1, for the method for Chinese caterpillar fungus rice wine, is characterized in that: in step (4), (8), (10), ground rice used is brown rice or powder of cracking rice.
5. simple bacterium fermentation continuously according to claim 1 is for the method for Chinese caterpillar fungus rice wine, it is characterized in that: step (7) obtained strains deposit number is the Cordyceps militaris (L.) Link. microparticle bacterial classification of NKHBCM209635, thalli granule particle diameter is 0.2 ~ 0.7mm, and cell concentration is 10
3~ 10
5individual/ml.
6. simple bacterium fermentation continuously according to claim 1 is for the method for Chinese caterpillar fungus rice wine, it is characterized in that: step (4) obtained strains deposit number is the Cordyceps militaris (L.) Link. microparticle bacterial classification of NKHBCM209635, Cordyceps militaris (L.) Link. thalline grain diameter is 0.3 ~ 0.5mm, and cell concentration is 5 × 10
3~ 10
4individual/ml.
7. simple bacterium fermentation continuously according to claim 1 is for the method for Chinese caterpillar fungus rice wine, it is characterized in that: in step (5), (6), access 2 ~ 3% Cordyceps militaris (L.) Link. microparticle liquid fungus seed in rice tank, lucifuge cultivates 5 ~ 7d, fermentation, see that light cultivates 3 ~ 5d, light intensity is 200 ~ 500Lux.
8. the simple continuously bacterium fermentation according to claim 1 or 7, for the method for Chinese caterpillar fungus rice wine, is characterized in that: in step (8), Rhizopus oryzae bacterium culture medium: ground rice 100 ~ 150g, potato leaching powder 30 ~ 40g, sucrose 20 ~ 30g, MgSO4/MgC
l20.5 ~ 1g, KH
2pO
4/ K
2hPO
42 ~ 3g, adds water to 1000ml; In temperature 28 DEG C, pH6.5, shears rotating speed 1000rpm, cultivates 40h.
9. simple bacterium fermentation continuously according to claim 1 is for the method for Chinese caterpillar fungus rice wine, it is characterized in that: step (8), (9) obtained strains deposit number are the Rhizopus oryzae bacterial classification grain diameter of CCTCCAF93150 is 0.2 ~ 0.5mm, and cell concentration is 10
3~ 10
5individual/ml.
10. simple bacterium fermentation continuously according to claim 1 is for the method for Chinese caterpillar fungus rice wine, it is characterized in that: in step (9), get 3 ~ 5% Rhizopus oryzae microparticle liquid spawns, add sterilized water and be diluted to 5 times, in the tank of access step (8), cultivate 36 ~ 40h for 28 DEG C.
11. simple bacterium fermentation continuously according to claim 1, for the method for Chinese caterpillar fungus rice wine, is characterized in that: step (8) obtained strains deposit number is the Rhizopus oryzae bacterial classification grain diameter of CCTCCAF93150 is 0.2 ~ 0.5mm, and cell concentration is 10
3~ 10
5individual/ml.
12. simple bacterium fermentation continuously according to claim 1 are for the method for Chinese caterpillar fungus rice wine, it is characterized in that: in step (10), yeast saccharomyces cerevisiae bacterial classification substratum: ground rice 150 ~ 200g, casein peptone 3 ~ 6g, Fructus Hordei Germinatus leaching powder 3 ~ 5g, sucrose 10 ~ 20g, yeast leaching powder 3 ~ 5g, adds water to 1000ml; Culture condition: temperature 28 DEG C, pH6.5, shears rotating speed 800rpm, cultivates 32h.
13. simple bacterium fermentation continuously according to claim 1, for the method for Chinese caterpillar fungus rice wine, is characterized in that: the yeast saccharomyces cerevisiae bacterial classification concentration that step (10) is cultivated is 5 × 10
6~ 10
7individual/ml.
14. simple bacterium fermentation continuously according to claim 1 are for the method for Chinese caterpillar fungus rice wine, it is characterized in that: step (11) gets 2 ~ 3% of screening meter Zhi Liang in step (1), the yeast saccharomyces cerevisiae bacterial classification that step (10) is cultivated.
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| CN106520565A (en) * | 2016-10-24 | 2017-03-22 | 蔡凤文 | Method for preparing cordyceps mycelium through glutinous rice |
| CN108566988A (en) * | 2018-07-04 | 2018-09-25 | 张斌 | Method for preparing novel selenium-rich rice and bean compound beverage |
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