CN107173467A - A kind of six fort tea aging methods - Google Patents
A kind of six fort tea aging methods Download PDFInfo
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- CN107173467A CN107173467A CN201710226137.2A CN201710226137A CN107173467A CN 107173467 A CN107173467 A CN 107173467A CN 201710226137 A CN201710226137 A CN 201710226137A CN 107173467 A CN107173467 A CN 107173467A
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- tea
- ageing
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- polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
Abstract
The invention discloses a kind of six forts tea aging method, it comprises the following steps:Sterilization sterilizing is first carried out to six fort tea using gamma-rays before the ageing of six fort tea, then adds ageing after ageing bacterium, the growth to be aged bacterium provides suitable nutrient environment and adds polysaccharides of traditional Chinese medicine and organic nitrogen, and is aged in suitable environment.The present invention can promote to be aged the growth of bacterium, promote the secretion of extracellular microbial exoenzyme, further promote the conversion of various materials in tealeaves, improve the ageing effect of six fort tea, the quality of six fort tea of lifting " red, dense, old, alcohol ".
Description
Technical field
The present invention relates to a plant tea preparing technical field, and in particular to a kind of six forts tea aging method.
Background technology
" golden flower " of six fort tea is the basis of special health, and " golden flower " of tea interlayer is the foundation for judging quality of Liupu tea,
The process of growing dim of revolving door is not provided with the preparation technology of six fort tea, process of growing dim is produced in ageing.Now research shows,
The golden yellow coloured particles of " golden flower " i.e. on tealeaves are the cleistotheciums that bulk bacteria are produced, and it is tea quality and the key factor of local flavor,
Through research, the strain for producing " golden flower " is mainly coronoid process dissipate capsule bacterium, and coronoid process dissipate capsule bacterium has vegetative propagation and generative propagation, " golden flower "
It is exactly the ascospore that coronoid process dissipate capsule bacterium generative propagation is produced, people are sent out the rule and optimum growh of the production spore of coronoid process dissipate capsule bacterium
The condition of educating have also been made research.Research shows that the optimum condition that coronoid process dissipate capsule bacterium produces spore on culture medium is sucrose 5%, ammonium nitrate
0.7%th, potassium chloride 3%, 28 DEG C of cultivation temperature, pH value are 7.In tealeaves, the nutriment of tealeaves and extraneous condition of culture
Similarly influence coronoid process produces the production spore process of spore bacterium.
It is another that there are some researches show " golden flower " is the cleistothecium that coronoid process dissipate capsule bacterium is produced, and influential on the quality of tea main
It is coronoid process dissipate capsule bacterium.Coronoid process dissipate capsule bacterium produces various ectoenzymes, such as polyphenol oxidase, pectase, cellulase, protease through it
Etc. as effective biochemical power, go various related substanceses in catalysis tealeaves to occur oxidation, polymerization, degraded, conversion, thus after
Continuing catalysis catechin oxidation makes soup look formation in amber, red dense intensification, can be also monose, catalysis by the Starch Conversion in tealeaves
Polyphenol compound is aoxidized, and makes that millet paste flavour is pure and sweet, and aftertaste is promoted the production of body fluid, and whole tealeaves is eliminated thick old taste, and comprehensive mouthfeel becomes taste
Taste is dense, fresh alcohol, and with pleasantly sweet, soup look reddens.Suitable an ageing environment and aging condition is thus provided particularly must
Will.
The content of the invention
Instant invention overcomes the shortcoming of above-mentioned technical problem, there is provided a kind of most suitable coronoid process dissipate capsule bacterium growth, ageing
Six good fort tea aging methods of effect.
To achieve the above object, the present invention takes following scheme:
A kind of six fort tea aging methods comprise the following steps:Will prepare ageing six fort tea γ -60Co or γ -
After the sterilization of 137Cs radiation exposures, the 0.1~0.5% of addition tea quality is aged the 0.01~0.1% of bacterium solution and tea quality
Organic nitrogen is mixed;The water containing polysaccharides of traditional Chinese medicine is sprayed, the water content for controlling tealeaves is 10~40%;Tealeaves is placed in closed
In space, oxygen is filled with, and is 60~70% in oxygen content, humidity is 70~80%, and temperature is permanent under the conditions of 23 DEG C~60 DEG C
Constant temperature and humidity is aged 7~30d;During ageing, with the water content and the humidity of air of the water management tealeaves containing polysaccharides of traditional Chinese medicine.
Wherein, the dosage of described γ -60Co or γ -137Cs radiation sterilizations is 1~15KGY.
Wherein, the organic nitrogen is preferably threonine, glutamine, Beta-alanine, can also be lysine, different bright ammonia
Acid, phenylalanine, tryptophan, valine, glycine, serine etc..
Wherein, described ageing bacterium solution is E.amstelodami, crawls to spread capsule bacterium, red bulk bacteria, thanks to a watt bulk bacteria
One or more and coronoid process dissipate capsule bacterium and yellow mould mix;Be aged bacterium solution specifically can also by E.amstelodami,
Coronoid process dissipate capsule bacterium, crawl to spread capsule bacterium, red bulk bacteria, thank a watt bulk bacteria, yellow mould and mix, also can by coronoid process dissipate capsule bacterium, crawl
Crawl, which spreads capsule bacterium, red bulk bacteria, thank a watt bulk bacteria, yellow mould mixes, also can by coronoid process dissipate capsule bacterium, red bulk bacteria, thank and watt dissipate
Capsule bacterium, yellow mould mix, and can also be mixed by coronoid process dissipate capsule bacterium, red bulk bacteria and yellow mould, but not limited to this is several
Combination.
Further, in described ageing bacterium solution, ageing bacterium cell concentration is 1 × 106~108。
Wherein, to be preferably Chinese caterpillar fungus polysaccharide, Ginkgo biloba polysaccharide, GL-B, the bark of eucommia more for described polysaccharides of traditional Chinese medicine
Sugar, pachymaran.
Further, in nature, it can find there is coronoid process dissipate capsule bacterium in cordyceps sinensis, ginkgo leaf, ganoderma lucidum, the bark of eucommia, Poria cocos,
Chinese caterpillar fungus polysaccharide by mannose, cordycepin, adenosine, galactolipin, arabinose, xylose essence, glucose, fucose constitute it is many
Glycan, with stronger pharmacological activity;Ginkgo biloba polysaccharide mainly contains rhamnose, a small amount of arabinose, mannose and gala
Sugar;In GL-B in addition to containing glucose, also containing arabinose, xylose, galactolipin, fucose, mannose, rhamnose etc.
Monose, par-tial polysaccharide also contains peptide chain;Contain gutta-percha, Pinoresinol diglucoside in eucommia bark polycose;Pachymaran mainly contains
Have pachyman, pachymaran, callose H11, containing a small amount of ergosterol, octanoic acid, hendecanoic acid, laurate,
The compounds such as lauric acid ester, palmitic acid, dodecylene acid esters, aprylic acid;The present invention is more by Chinese caterpillar fungus polysaccharide, ginkgo leaf
Sugar, GL-B, eucommia bark polycose, pachymaran are combined, and carbohydrate is various in style, while having high pharmacological activity, it cooperates,
Early stage provides nutriment for ageing bacterium, promotes growing dim for tealeaves, and later stage ageing bacterium produces polyphenol oxidase, pectase, fiber
After a variety of ectoenzymes such as plain enzyme, protease, polysaccharides of traditional Chinese medicine is used a variety of enzymes, anti-with material in tealeaves
Should, promote the conversion between each material in tealeaves, lift the nutritive value and health-care effect of tealeaves.
Further, in the water containing polysaccharides of traditional Chinese medicine, the mass percent 1~5% of polysaccharides of traditional Chinese medicine.
Wherein, described polysaccharides of traditional Chinese medicine is extracted by following methods and obtained:By cordyceps sinensis, ginkgo leaf, ganoderma lucidum, the bark of eucommia,
Poria cocos is ground into the pulvis that particle diameter is less than 800um, then Chinese herbal medicine is carried out into ungrease treatment 10h with 95% ethanol reagent;By degreasing
Obtained pulvis after processing carries out refluxing extraction with water and obtains Aqueous extracts;Aqueous extracts are put again and precipitated with absolute ethyl alcohol,
Precipitation is washed with ether, acetone and absolute ethyl alcohol successively, polysaccharides of traditional Chinese medicine is obtained.
The property of medicine for the polysaccharides of traditional Chinese medicine that the present invention is used is as follows:
Cordyceps sinensis, it is sweet, it is warm-natured flat, nontoxic, it is famous strengthening by means of tonics medicine, the effect for having qi-restoratives to be healthy and strong.
Ginkgo leaf, sweet, bitter, puckery, mild-natured, the thoughts of returning home, lung channel;With astringing the lung, relieving asthma, it is promoting blood circulation and removing blood stasis, the effect of analgesic.
Ganoderma lucidum, it is warm-natured, it is lightly seasoned, with invigorating qi for tranquilization, relieving cough and asthma effect.
The bark of eucommia, it is sweet, it is warm-natured, tonify the liver and kidney, strengthen muscles and bones, coordinating Chong and Conception Vessels, strengthen meridian and miscarriage prevention.
Poria cocos, sweet, property is light flat, enters the heart, lung, the spleen channel;With eliminating dampness and diuresis, strengthening the spleen and stomach, effect of antitoxic heart-soothing and sedative.
The present invention has the advantages that compared with prior art:
1st, aging method of the invention first carries out sterilization sterilizing to six fort tea using gamma-rays, then adds old after ageing bacterium
Change, while adding polysaccharides of traditional Chinese medicine and organic nitrogen, the growth for ageing bacterium provides suitable nutrient environment, greatly improves ageing bacterium
Growth, promote extracellular microbial exoenzyme secretion, further promote tealeaves in various materials conversion, improve six fort tea ageing
Effect.
2nd, the present invention is sterilized using γ -60Co or γ -137Cs to microorganism, can all kill in six fort tea and own
Microbial bacteria, for ageing when microorganism development provide one without miscellaneous bacteria competition ageing environment;
3rd, present invention addition organic nitrogen, for the abundant nitrogen source that provides of ageing bacterium, the threonine of addition, glutamine, β-
Alanine can promote E.amstelodami, crawl to spread capsule bacterium, red bulk bacteria, thank a watt scattered capsule, coronoid process dissipate capsule bacterium, yellow mould
Growth and breeding, contribute to six fort tea ageings;
4th, present invention addition polysaccharides of traditional Chinese medicine, provides abundant carbon source, while not influenceing the quality of six fort tea for ageing bacterium
And local flavor, polysaccharides of traditional Chinese medicine is also with specific Chinese herbal medicine medical value, the especially health-care effect of six fort tea of lifting, winter worm summer
Grass polysaccharide, Ginkgo biloba polysaccharide, GL-B, eucommia bark polycose, pachymaran can promote to be aged the growth and breeding of bacterium, contribute to six forts
The ageing of tea.
Embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1
A kind of six fort tea aging methods comprise the following steps:Six fort tea γ -60Co rays of ageing will be prepared to kill
After microbial inoculum amount is 15KGY irradiation-sterilizes, addition tea quality 0.1% by E.amstelodami, crawl to spread capsule bacterium, it is red
Bulk bacteria, to thank to the cell concentration that a watt bulk bacteria, coronoid process dissipate capsule bacterium and yellow mould mix be 1 × 108Ageing bacterium solution and tea
The organic nitrogen being made up of threonine, glutamine, Beta-alanine of the 0.01% of leaf quality is mixed;Sprinkling is more by cordyceps sinensis
The polysaccharides of traditional Chinese medicine water that sugar, Ginkgo biloba polysaccharide, GL-B, eucommia bark polycose, the mass percent of pachymaran composition are 5%,
The water content for controlling tealeaves is 10%;Tealeaves is placed in confined space, oxygen is filled with, and is 70%, humidity in oxygen content
70%, temperature constant temperature and humidity under the conditions of 60 DEG C is aged 7d;During ageing, with the water management tealeaves containing polysaccharides of traditional Chinese medicine
The humidity of water content and air.
Wherein, described polysaccharides of traditional Chinese medicine is extracted by following methods and obtained:By cordyceps sinensis, ginkgo leaf, ganoderma lucidum, the bark of eucommia,
Poria cocos is ground into the pulvis that particle diameter is less than 800um, then Chinese herbal medicine is carried out into ungrease treatment 10h with 95% ethanol reagent;By degreasing
Obtained pulvis after processing carries out refluxing extraction with water and obtains Aqueous extracts;Aqueous extracts are put again and precipitated with absolute ethyl alcohol,
Precipitation is washed with ether, acetone and absolute ethyl alcohol successively, polysaccharides of traditional Chinese medicine is obtained.
Embodiment 2
A kind of six fort tea aging methods comprise the following steps:Six fort tea γ -137Cs rays of ageing will be prepared to kill
Microbial inoculum amount be 1KGY irradiation-sterilizes after, addition tea quality 0.5% by E.amstelodami, coronoid process dissipate capsule bacterium and Huang
The cell concentration that mould mixes is 1 × 106Ageing bacterium solution and tea quality 0.1% by threonine, glutamine,
The organic nitrogen of Beta-alanine composition is mixed;Sprinkling contains many by Chinese caterpillar fungus polysaccharide, Ginkgo biloba polysaccharide, GL-B, the bark of eucommia
The polysaccharides of traditional Chinese medicine water that sugar, the mass percent of pachymaran composition are 1%, the water content for controlling tealeaves is 40%;By tea
Leaf is placed in confined space, is filled with oxygen, and is 60% in oxygen content, and humidity is 80%, and temperature constant temperature under the conditions of 23 DEG C is permanent
Wet ageing 30d;During ageing, with the water content and the humidity of air of the water management tealeaves containing polysaccharides of traditional Chinese medicine.
Wherein, the extracting method be the same as Example 1 of described polysaccharides of traditional Chinese medicine.
Embodiment 3
A kind of six fort tea aging methods comprise the following steps:Six fort tea γ -137Cs rays of ageing will be prepared to kill
After microbial inoculum amount is 12KGY irradiation-sterilizes, addition tea quality 0.3% by E.amstelodami, crawl to spread capsule bacterium, hat
The cell concentration that prominent bulk bacteria and yellow mould mix is 1 × 107Ageing bacterium solution and tea quality 0.05% by reviving
Propylhomoserin, glutamine, the organic nitrogen of Beta-alanine composition are mixed;Sprinkling contains by Chinese caterpillar fungus polysaccharide, Ginkgo biloba polysaccharide, spirit
The polysaccharides of traditional Chinese medicine water that sesame polysaccharide, eucommia bark polycose, the mass percent of pachymaran composition are 3%, controls the water content of tealeaves
For 30%;Tealeaves is placed in confined space, oxygen is filled with, and is 65% in oxygen content, humidity is 75%, and temperature is in 30 DEG C of bars
Constant temperature and humidity is aged 15d under part;During ageing, with the water content and the humidity of air of the water management tealeaves containing polysaccharides of traditional Chinese medicine.
Wherein, the extracting method be the same as Example 1 of described polysaccharides of traditional Chinese medicine.
Comparative example 1
The comparative example 1 of the present invention and the six fort tea aging methods that embodiment 1 is provided are essentially identical, and difference is, comparative example 1
It is to be sterilized without γ -60Co or γ -137Cs radiation exposures before ageing.
Comparative example 2
The comparative example 2 of the present invention and the six fort tea aging methods that embodiment 2 is provided are essentially identical, and difference is, comparative example 2
The water containing polysaccharides of traditional Chinese medicine is replaced to spray tealeaves with light water.
Comparative example 3
The comparative example 3 of the present invention and the six fort tea aging methods that embodiment 3 is provided are essentially identical, and difference is, comparative example 3
Without organic nitrogen.
Comparative example 4
Comparative example 4 and the six fort tea aging methods that embodiment 3 is provided are essentially identical, and difference is, comparative example 4 is only with containing
The water sprinkling tealeaves of eucommia bark polycose.
The comparative example 5 of comparative example 5 uses the common aging method of traditional handicraft.
In order to illustrate the present invention technique effect, according to the embodiment of the present invention 1, embodiment 2, embodiment 3 and comparative example 1,
The six fort tea aging methods that comparative example 2, comparative example 3, comparative example 4, comparative example 5 are provided, every kind of six forts tea aging method ageing
50kg, detects that the water extraction, Tea Polyphenols, coarse-fibred content of six fort tea are recorded in table 1 respectively after the completion of ageing, and to old
Tea after change carries out the organoleptic detections such as fragrance, tea residue, flavour, millet paste color and recorded in table 2;Water extraction detection is with reference to country
Standard method GB/T8305-2002, Tea Polyphenols detection refers to national standard method GB/T8313-2008, the inspection of crude fiber content
Survey and refer to national standard method GB/T8310-2002, fragrance detection uses Headspace-solid phase microextraction-gas chromatography mass spectrometry method (HS-
SPME-GC-MS Chen Xiang note characteristic flavor compounds methoxybenzene class content of material) is monitored.
Table 1
Table 2
Research shows that the quality of six fort tea and the chemical composition that tea is included are closely bound up, and the content of the water extraction of tea is got over
Height, tea leaf quality is higher, and crude fiber content is higher, and tea leaf quality is poorer;Tea Polyphenols oxidation polymerization shape in tea ageing process
Into brown oxide, change millet paste color and luster, improve the alcohol thickness of millet paste.
As shown in Table 1, using the aging method of offer of the invention, its water extraction does not take γ compared to comparative example 1
X ray irradiation x, comparative example 2 without polysaccharides of traditional Chinese medicine, comparative example 3 without organic nitrogen, that comparative example 4 adds the single bark of eucommia is many
Sugar and the common aging method of comparative example 5 are all high, and its Tea Polyphenols and coarse-fibred content are all relatively low, illustrate six fort tea ageings
During, using gamma-ray irradiation and a variety of polysaccharides of traditional Chinese medicine and organic nitrogen are added, can promote to be aged the growth of bacterium and grow dim, enter
One step promotes various related substanceses in six fort tea to occur oxidation, polymerization, degraded, conversion, improves the quality of tealeaves.
As shown in Table 2, the six fort tea aging methods provided using the present invention have excellent compared to 1~comparative example of comparative example 5
Good color and luster, fragrance, the dense Chen Xiang of tealeaves, the six fort tea that the six fort tea aging methods that 1~comparative example of comparative example 5 is provided are obtained are not
Enough old, inadequate alcohol, illustrates that the six fort tea aging methods that the present invention is provided can make six fort tea have " red, dense, the old, alcohol " of high-quality special
Color.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
The equal change or modification change completed under bright patent claim, the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of six forts tea aging method, it is characterised in that comprise the following steps:Will prepare ageing six fort tea γ-
After the sterilization of 60Co or γ -137Cs radiation exposures, 0.1~0.5% ageing bacterium solution of addition tea quality and the 0.01 of tea quality
~0.1% organic nitrogen is mixed;The water containing polysaccharides of traditional Chinese medicine is sprayed, the water content for controlling tealeaves is 10~40%;By tea
Leaf is placed in confined space, is filled with oxygen, and is 60~70% in oxygen content, and humidity is 70~80%, and temperature is 23 DEG C~60
Constant temperature and humidity is aged 7~30d under the conditions of DEG C;During ageing, with the water content and sky of the water management tealeaves containing polysaccharides of traditional Chinese medicine
The humidity of gas;
Wherein, described ageing bacterium solution is by E.amstelodami, crawls to spread capsule bacterium, red bulk bacteria, thanks to watt bulk bacteria one
Kind or a variety of mixed with Huang mould with coronoid process dissipate capsule bacterium.
2. a kind of six forts tea aging method according to claim 1, it is characterised in that described polysaccharides of traditional Chinese medicine is winter worm
Summer grass polysaccharide, Ginkgo biloba polysaccharide, GL-B, eucommia bark polycose, pachymaran.
3. a kind of six forts tea aging method according to claim 1, it is characterised in that described organic nitrogen be threonine,
Glutamine and Beta-alanine.
4. a kind of six forts tea aging method according to claim 1, it is characterised in that in the water containing polysaccharides of traditional Chinese medicine,
The mass percent 1~5% of polysaccharides of traditional Chinese medicine.
5. a kind of six forts tea aging method according to claim 1, it is characterised in that described γ -60Co or γ -
The dosage of 137Cs radiation sterilizations is 1~15KGY.
6. a kind of six forts tea aging method according to claim 1 or 2, it is characterised in that old in described ageing bacterium solution
It is 1 × 10 to change bacterium cell concentration6~108。
7. a kind of six forts tea aging method according to claim 2, it is characterised in that:Described polysaccharides of traditional Chinese medicine is by following
Method is extracted and obtained:Cordyceps sinensis, ginkgo leaf, ganoderma lucidum, the bark of eucommia, Poria cocos are ground into particle diameter and are less than 800um pulvis, then is used
Chinese herbal medicine is carried out ungrease treatment 10h by 95% ethanol reagent;Obtained pulvis after ungrease treatment is subjected to refluxing extraction with water
Obtain Aqueous extracts;Aqueous extracts are put again and precipitated with absolute ethyl alcohol, precipitation is washed with ether, acetone and absolute ethyl alcohol successively
Wash, obtain polysaccharides of traditional Chinese medicine.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110800822A (en) * | 2019-11-26 | 2020-02-18 | 普定县久茗茶业有限公司 | Method for preparing white tea |
CN112772738A (en) * | 2020-12-28 | 2021-05-11 | 溧阳市龙昇茶叶专业合作社 | Preparation method of theanine-rich white-leaf dark tea capable of reducing blood fat and blood pressure |
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