CN103190576B - Cordyceps militaris soybean and preparation method thereof - Google Patents
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Abstract
本发明涉及一种蛹虫草黄豆，是将黄豆用水进行浸泡，浸泡后的黄豆进行灭菌，冷却后接入蛹虫草菌的种子液进行发酵培养，再发酵结束后风干得到蛹虫草黄豆。蛹虫草在黄豆中进行生物转化时，黄豆的种皮对其有适当的抑制作用。因此，对浸泡黄豆进行去皮处理，能够提高其发酵后的虫草菌素含量。另外，增大而均匀接种面积，可以提高蛹虫草虫草菌素的生物转化量。故通过纤维素酶处理，提高对浸泡黄豆的去皮率，并将去皮后的黄豆，提高蛹虫草黄豆中的虫草菌素转化量。The invention relates to soybeans of Cordyceps militaris, which comprises soaking soybeans in water, sterilizing the soaked soybeans, adding seed liquid of Cordyceps militaris after cooling for fermentation and cultivation, and air-drying soybeans of Cordyceps militaris to obtain soybeans of Cordyceps militaris. When Cordyceps militaris undergoes biotransformation in soybean, the seed coat of soybean has an appropriate inhibitory effect on it. Therefore, peeling soaked soybeans can increase the content of cordycepin after fermentation. In addition, increasing and uniform inoculation area can increase the biotransformation amount of Cordycepin militaris. Therefore, through cellulase treatment, the peeling rate of soaked soybeans is improved, and the peeled soybeans are used to increase the conversion of cordycepin in Cordyceps militaris soybeans.
Description
Technical field
The invention belongs to technical field of food preparation, be specifically related to a kind of Cordyceps militaris soya bean and preparation method thereof.
Background technology
Cordyceps militaris (Cordyceps militaris) is Ascomycotina, gang pyrenomycetes, and Hypocreales, Clavicipitaceae, kind of Cordyceps, for belonging to xenogenesis fungi together, is commonly referred to northern Chinese caterpillar fungus with Cordyceps sinensis (Cordyceps sinensis).Owing to thering is identical medicinal and nourishing function with Cordyceps sinensis, and be easier to carry out artificial cultivation than Cordyceps sinensis, therefore Cordyceps militaris is chosen as the best substitute of Cordyceps sinensis and carries out a large amount of artificial cultures, and comes into the market as the approval of new resource food and a kind new medicine Yi Huo Ministry of Public Health and State Food and Drug Administration.
The active component of Cordyceps militaris comprises cordycepin, adenosine, Cordyceps sinensis polysaccharide and cordycepic acid etc., and the wherein research to cordycepin, becomes focus both domestic and external in recent years.Cordycepin (Cordycepin) claim again cordycepin, being cordycepin (3 '-Deoxyadenosine), is a kind of nucleoside antibiotic, has multiple physiologically active, as antibacterial, antitumor, antiviral effect, and strengthen the effects such as immunologic function and control cardiovascular and cerebrovascular disease.
The special medical health care of cordycepin has caused domestic and international expert's great attention, existing much take cordycepin and puts on market as main health products, health food, cosmetics, medicine.It is reported, in the U.S., cordycepin is entered to clinic trial as anticancer new antiviral drug.In China, also the leukemic new drug of the treatment of being synthesized by cordycepin is entered to first phase clinic trial.
The pure fructification of the artificial Cordyceps militaris of cultivating, as food, be used for human body, completely after deliberation, can be widely used in health food, health diet and other nourishing based food, such as making cordycepin beverage, health liquor, nutritious health-care vinegar etc., but be mostly that to take fructification or solid medium leaching liquor be raw material, technique relative complex, cost is higher.Along with the progress of foods processing technique and people are to the deepening continuously of Chinese caterpillar fungus research, Chinese caterpillar fungus functional food should be to the future development of diversification, process meticulousr, more scientific, the function of filling a prescription more clearly, more remarkable effect.And by the approach of bio-transformation, the cordycepin not having in original food is generated, and it is processed into food, meeting the requirement of 21 century people to food, development prospect is wide.
Patent CN101171968A discloses by cereal solid-state fermentation Chinese caterpillar fungus and ganoderma lucidum product and manufacture method thereof, to utilize the different cereal such as heart of a lotus seed benevolence, rice, wheat, oat or feed as the tunning of Cordyceps sinensis or glossy ganoderma solid state fermentation, by the dry pulverize of this tunning, can add again flour and flavor enhancement in addition, can be made into health-care components such as being rich in dietary fiber, polysaccharide, and the Cordyceps sinensis or glossy ganoderma lotus-seed-heart powder, ground rice, wheat flour or the oatmeal that facilitate consumer to brew to drink.
At present, with Chinese caterpillar fungus, soya bean is carried out to bio-transformation, in the solid fermentation of soya bean, produce the research of cordycepin, at home and abroad there are no report.In China builds a well-off society in an all-round way, if by functional components such as the bio-transformation of soya bean and the approach of serial converted products picked-up cordycepins thereof, to improving human health, significant, prospect is very wide.
Summary of the invention
The object of this invention is to provide a kind of Cordyceps militaris soya bean and preparation method thereof, thereby make up the deficiencies in the prior art.
Cordyceps militaris soya bean of the present invention is to using soya bean as solid medium, inoculation cordyceps cultivation preparation.
Cordyceps militaris soya bean of the present invention, its a kind of preparation method is as follows: soya bean water is soaked, and the soya bean after immersion carries out sterilizing, and the seed liquor of cooling rear access cordyceps is carried out fermented and cultured, more air-dryly after fermentation ends obtains Cordyceps militaris soya bean.
The seed liquor of cordyceps of the present invention, its seed culture medium of preparing use is composed as follows: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%, surplus is water.
Above-mentioned seed liquor, is also added with Eupolyphaga sinensis peptide mixer, and the concentration of interpolation is 0.005%.
Described Eupolyphaga sinensis peptide mixer, is that Eupolyphaga sinensis powder is added after distilled water, with papain, carries out enzymolysis, prepared by enzymolysis liquid filtration drying.
Above-mentioned fermented and cultured, is that cultivated after 5～8 days in darkroom at 22 ℃, then in bright chamber, cultivates and within 3～6 days, completes fermentation.
In order to obtain better ferment effect, the soya bean of soaking is processed with enzyme before sterilizing;
Described processes with enzyme, is that the soya bean to soaking adds the citric acid-sodium hydrogen phosphate buffer solution (pH is 4.5) that is added with cellulase to carry out enzymolysis;
Described cellulose enzyme concentration is 8000～12000U/g soya bean, carries out enzyme at 50 ℃ and processes 1～3 hour.
The present invention also provides a kind of Cordyceps militaris culture mix, is Cordyceps militaris soya bean and Cordyceps militaris peanut mixt.
When Cordyceps militaris carries out bio-transformation in soya bean, the kind skin of soya bean has inhibitory action to it.Therefore, to soaking soya bean, remove the peel processing, can improve the cordycepin content after its fermentation.Therefore process by cellulase, reach the object of soya bean peeling, thereby improve the cordycepin inversion quantity in Cordyceps militaris soya bean.
The specific embodiment
Cordyceps of the present invention (Cordyceps militaris) can be selected the bacterial classification of the cordyceps of applying in arbitrary field of food.And in order to make the vigor of cordyceps in culture medium higher, the present invention has carried out long-term research to the component of culture medium, provide a kind of culture medium that can obviously strengthen cordyceps vigor.
Below in conjunction with embodiment, Cordyceps militaris soya bean of the present invention and preparation method thereof is described in detail.
Embodiment 1
1) soybean soaking
Take the soya bean of 250g, added the water of 500g, at room temperature soak 12h, the soya bean water content after soaking is controlled in 100～120% scopes.
2) sterilizing
After soya bean after soaking is drained, minute install in triangular flask, build stopper, at 121 ℃ of temperature, sterilizing 30 minutes, waits to inoculate after cooling.
3) Cordyceps militaris spawn inclined-plane is cultivated
The Cordyceps militaris spawn of keeping is inoculated on slant medium, at the temperature of 23 ℃, cultivated 7～9 days, consisting of of its slant medium: potato 18～22%, glucose 1～3%, peptone 0.1～1.0%, potassium dihydrogen phosphate 0.1～0.5%, magnesium sulfate 0.1～0.3%, Cobastab
10.008～
?0.02%, agar 1～3%.Wherein concrete a kind of formula is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.25%, Cobastab
10.01%, agar 2%.The method of preparation, is by 20g potato cutting, adds 100mL water, boil 20 minutes, boil with cooling procedure in constantly moisturizing, filter after being finally adjusted to 100mL.By 2g glucose, 0.5g peptone, 0.3g potassium dihydrogen phosphate, 0.25g magnesium sulfate, 0.01g Cobastab
1, 2g agar is dissolved in 100mL potato filtrate.
4) cultivation of Cordyceps militaris seed liquor
Get area and reach 10～30 ㎜
2inclined-plane Cordyceps militaris spawn, receive in seed liquor culture medium, at the temperature of 20～25 ℃, cultivate and within 2～6 days, obtain seed liquor.The mass percent of its liquid seed culture medium consists of: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%.
Wherein concrete a kind of formula is as follows: potato 20%, glucose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.1%.The method of preparation, is by 20g potato cutting, adds 100mL water, boil 20 minutes, boil with cooling procedure in constantly moisturizing, filter after being finally adjusted to 100mL.By 2g glucose, 0.2g peptone, 0.05g potassium dihydrogen phosphate, 0.1g magnesium sulfate, be dissolved in 100mL potato filtrate.
In order better to improve the fermentative activity of the cordyceps of cultivating, in seed liquor culture medium, add Eupolyphaga sinensis peptide mixer, add quality concentration of volume percent 0.005%, in 100ml culture medium, add the Eupolyphaga sinensis peptide mixer of 0.005g.Wherein a kind of preparation method of Eupolyphaga sinensis peptide mixer is as follows:
Get the Eupolyphaga sinensis powder of 20g after freeze drying, add 400ml distilled water, with 10%NaOH, regulate pH value to 8.2, then by 300U/g raw material, add papain, enzymolysis 5h at 37 ℃.Enzymolysis liquid, at 80 ℃, is gone out after enzyme 15min, and the centrifugal 15min of rotating speed with 10000r/min, gets supernatant, obtains Eupolyphaga sinensis enzymolysis liquid.
For obtaining low-molecular-weight peptide mixer, the supernatant of enzymolysis liquid is filtered with 2500Da molecular weight milipore filter, remove macro-molecular protein, collect filtered solution, with 250Da molecular weight milipore filter, filter again, remove small molecule salt class, collect trapped fluid and concentrate, freeze drying, obtains the Eupolyphaga sinensis mixtures of polypeptides that molecular weight is less than 2500Da.
5) expansion of Cordyceps militaris seed liquor is cultivated
By above-mentioned Cordyceps militaris seed liquor, by 8～15% volume ratio, receive and expand in liquid seeds liquid culture medium, at the temperature of 20～25 ℃, carry out Cordyceps militaris and expand cultivation 2～6 days.
6) solid culture of Cordyceps militaris soya bean
Cultured Cordyceps militaris is expanded to seed liquor, by 10～25%(v/w) inoculum concentration receive after sterilizing on cooling good soya bean, at 22 ℃, cultivated after 5～8 days in darkroom, then bright chamber cultivation 3～6 days; The brightness of described bright chamber is exactly 16 watts of the incandescent lamp power that arrange in IS-RDU3 model isothermal vibration device.In the incubation of bright chamber, suitably stir.
7) dry
Cordyceps militaris soya bean after fermentation ends, is yellow～orange-yellow, with natural wind, dries in the shade, and obtains Cordyceps militaris soya bean finished product.
8) cordycepin content assaying method
Adopt high performance liquid chromatography.
The extraction of cordycepin: by the Cordyceps militaris soya bean crushing, sieve after (60 order), add 20% ethanol by the solid-liquid ratio of 1:20, extract 30～60 minutes in ultrasonic device (40KHz, 40 ℃).After cordycepin extracts and to finish, under 10000rpm rotating speed centrifugal 10 minutes.To utilize supernatant after 0.22um membrane filtration as the testing sample of measuring cordycepin.
Liquid-phase condition is that pillar is used KnauerC
18; Column temperature, room temperature; Detect wavelength, 260nm; Mobile phase, acetonitrile: water=10:90; Flow velocity: 0.4mL/min.
Cordycepin content in Cordyceps militaris soya bean prepared by the present embodiment, at 10～13g/kg, is compared and is increased to respectively 2～5 times and 15～20 times with the cordycepin content in silkworm chrysalis Cordyceps sinensis with rice Cordyceps militaris.
And by adding Eupolyphaga sinensis peptide mixer, the cordycepin in the soya bean of follow-up preparation is increased, and testing result shows, adds Eupolyphaga sinensis peptide mixer than in the situation of not adding, the content of the cordycepin in soya bean has 25～40% lifting.
In culture medium, the kind of nitrogenous source, concentration and catabolite thereof all can significantly affect cometabolism, and contain nitrogen-atoms in the molecular structure of many secondary metabolites, therefore nitrogenous source is except as forming the material of somatic cells, also can be directly as the precursor of secondary metabolite.Inorganic nitrogen-sourced because its composition is single, significant adverse is synthetic in Growth of Cells and metabolite, and while adopting compound nitrogen source, complexity and changeability because of its composition, have great importance to the anabolism of cordycepin.
Embodiment 2
1) soybean soaking
Take the soya bean of 250g, in the ratio of 1:2～1:5, add water, at room temperature soak 10～16h, make soya bean water content be controlled at 100～120%.
2) soya bean cellulase is processed
By soaked soya bean, after draining, be encased in triangular flask and add citric acid-sodium hydrogen phosphate buffer solution by 1:3～5 solid-liquid ratio, at 50 ℃, carrying out cellulase processes 1～3 hour, enzyme concentration is 8000～12000U/g soya bean, with flowing water flushing, after 10～20 minutes, drain, drying in the shade, place is dried 5～8 hours.Cellulase derives from green wooden enzyme, and vigor is 11000U/mg.
3) sterilizing
10～15 orders peelings soya bean particles, minute install in triangular flask, build stopper, and at 121 ℃ of temperature, sterilizing 30 minutes, waits to inoculate after cooling.
4) Cordyceps militaris spawn inclined-plane is cultivated
The Cordyceps militaris spawn of keeping is inoculated on slant medium, at the temperature of 23 ℃, cultivated 7～9 days, consisting of of its slant medium: potato 18～22%, glucose 1～3%, peptone 0.1～1.0%, potassium dihydrogen phosphate 0.1～0.5%, magnesium sulfate 0.1～0.3%, Cobastab
10.008～
?0.02%, agar 1～3%.Wherein concrete a kind of formula is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.25%, Cobastab
10.01%, agar 2%.The method of preparation, is by 20g potato cutting, adds 100mL water, boil 20 minutes, boil with cooling procedure in constantly moisturizing, filter after being finally adjusted to 100mL.By 2g glucose, 0.5g peptone, 0.3g potassium dihydrogen phosphate, 0.25g magnesium sulfate, 0.01g Cobastab
1, 2g agar is dissolved in 100mL potato filtered fluid.
5) cultivation of Cordyceps militaris seed liquor
Get area and reach 10～30 ㎜
2inclined-plane Cordyceps militaris spawn, receive in seed liquor culture medium, at the temperature of 20～25 ℃, cultivate and within 2～6 days, obtain seed liquor.The mass percent of its liquid seed culture medium consists of: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%.
Wherein concrete a kind of formula is as follows: potato 20%, glucose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.1%.The method of preparation, is by 20g potato cutting, adds 100mL water, boil 20 minutes, boil with cooling procedure in constantly moisturizing, filter after being finally adjusted to 100mL.By 2g glucose, 0.2g peptone, 0.05g potassium dihydrogen phosphate, 0.1g magnesium sulfate, be dissolved in 100mL potato filtered fluid.
6) expansion of Cordyceps militaris seed liquor is cultivated
By above-mentioned Cordyceps militaris seed liquor, by 8～15% volume ratio, receive and expand in liquid seeds liquid culture medium, at the temperature of 20～25 ℃, carry out Cordyceps militaris and expand cultivation 2～6 days.
In order better to improve the fermentative activity of the cordyceps of cultivating, in seed liquor culture medium, add Eupolyphaga sinensis peptide mixer, add mass percent concentration 0.005%, in 100ml culture medium, add the Eupolyphaga sinensis peptide mixer of 0.005g.Wherein a kind of preparation method of Eupolyphaga sinensis peptide mixer is as follows:
Get the Eupolyphaga sinensis powder of 20g after freeze drying, add 400ml distilled water, with 10%NaOH, regulate pH value to 8.2, then by 300U/g raw material, add papain, enzymolysis 5h at 37 ℃.Enzymolysis liquid, at 80 ℃, is gone out after enzyme 15min, and the centrifugal 15min of rotating speed with 10000r/min, gets supernatant, obtains Eupolyphaga sinensis enzymolysis liquid.
For obtaining low-molecular-weight peptide mixer, the supernatant of enzymolysis liquid is filtered with 2500Da molecular weight milipore filter, remove macro-molecular protein, collect filtered solution, with 250Da molecular weight milipore filter, filter again, remove small molecule salt class, collect trapped fluid and concentrate, freeze drying, obtains the Eupolyphaga sinensis mixtures of polypeptides that molecular weight is less than 2500Da.
7) solid culture of Cordyceps militaris soya bean
Cultured Cordyceps militaris is expanded to seed liquor, by 10～25%(v/w) inoculum concentration receive after sterilizing on cooling good soya bean, at 22 ℃, cultivated after 5～8 days in darkroom, then bright chamber cultivation 3～6 days; The brightness of described bright chamber is exactly 16 watts of the incandescent lamp power that arrange in IS-RDU3 model isothermal vibration device.In the incubation of bright chamber, suitably stir.
8) dry
Cordyceps militaris soya bean after fermentation ends, is yellow～orange-yellow, with natural wind, dries in the shade, and obtains Cordyceps militaris soya bean finished product.
Cordycepin content in Cordyceps militaris soya bean prepared by utilization peeling soya bean, at 15～17g/kg, is not compared with removing the peel soya bean, and cordycepin content promotes 30～50%.
Embodiment 3
1) soybean soaking
Take the soya bean of 250g, in the ratio of 1:2～1:5, add water, at room temperature soak 10～16h, make soya bean water content be controlled at 100～120%.
2) soya bean cellulase is processed
By soaked soya bean, after draining, be encased in triangular flask and add citric acid-sodium hydrogen phosphate buffer solution by 1:3～5 solid-liquid ratio, at 50 ℃, carrying out cellulase processes 1～3 hour, enzyme concentration is 8000～12000U/g soya bean, with flowing water flushing, after 10～20 minutes, drain, drying in the shade, place is dried 5～8 hours.
3) coupling of two kinds of composition solid mediums
After peanut after soya bean after cellulase is processed and peeling is mixed in the ratio of 1:0.1～1.0, then in the ratio of 2:0.5～2.5, add water and mix, subject to sterilization.
4) sterilizing
By two kinds of composition solid mediums that match by a certain percentage, build stopper, at 121 ℃ of temperature, sterilizing 30 minutes, waits to inoculate after cooling.
5) Cordyceps militaris spawn inclined-plane is cultivated
The Cordyceps militaris spawn of keeping is inoculated on slant medium, at the temperature of 23 ℃, cultivated 7～9 days, consisting of of its slant medium: potato 18～22%, glucose 1～3%, peptone 0.1～1.0%, potassium dihydrogen phosphate 0.1～0.5%, magnesium sulfate 0.1～0.3%, Cobastab
10.008～
?0.02%, agar 1～3%.Wherein concrete a kind of formula is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.25%, Cobastab
10.01%, agar 2%.The method of preparation, is by 20g potato cutting, adds 100mL water, boil 20 minutes, boil with cooling procedure in constantly moisturizing, filter after being finally adjusted to 100mL.By 2g glucose, 0.5g peptone, 0.3g potassium dihydrogen phosphate, 0.25g magnesium sulfate, 0.01g Cobastab
1, 2g agar is dissolved in 100mL potato filtered fluid.
6) cultivation of Cordyceps militaris seed liquor
Get area and reach 10～30 ㎜
2inclined-plane Cordyceps militaris spawn, receive in seed liquor culture medium, at the temperature of 20～25 ℃, cultivate and within 2～6 days, obtain seed liquor.The mass percent of its liquid seed culture medium consists of: potato 18～22%, glucose 1～3%, peptone 0.1～0.5%, potassium dihydrogen phosphate 0.03～0.1%, magnesium sulfate 0.05～0.5%.
Wherein concrete a kind of formula is as follows: potato 20%, glucose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.1%.The method of preparation, is by 20g potato cutting, adds 100mL water, boil 20 minutes, boil with cooling procedure in constantly moisturizing, filter after being finally adjusted to 100mL.By 2g glucose, 0.2g peptone, 0.05g potassium dihydrogen phosphate, 0.1g magnesium sulfate, be dissolved in 100mL potato filtered fluid.
7) expansion of Cordyceps militaris seed liquor is cultivated
By above-mentioned Cordyceps militaris seed liquor, by 8～15% volume ratio, receive and expand in liquid seeds liquid culture medium, at the temperature of 20～25 ℃, carry out Cordyceps militaris and expand cultivation 2～6 days.
In order better to improve the fermentative activity of the cordyceps of cultivating, in seed liquor culture medium, add Eupolyphaga sinensis peptide mixer, add mass percent concentration 0.005%.The preparation method of the Eupolyphaga sinensis peptide mixer wherein adding is as follows:
Get the degreasing Eupolyphaga sinensis powder of 20g after freeze drying, by 1:20 material-water ratio, add distilled water, with 10%NaOH, regulate pH value to 8.2, then press 300U/g raw material, add papain, enzymolysis 5h at 37 ℃.Enzymolysis liquid, at 80 ℃, is gone out after enzyme 15min, and the centrifugal 15min of rotating speed with 10000r/min, gets supernatant, obtains Eupolyphaga sinensis enzymolysis liquid.
For obtaining low-molecular-weight peptide mixer, the centrifugal supernatant obtaining of enzymolysis liquid is filtered with 2500Da molecular weight milipore filter, remove macro-molecular protein, collect filtered solution, with 250Da molecular weight milipore filter, filter again, remove small molecule salt class, collect trapped fluid and concentrate, freeze drying, obtains the Eupolyphaga sinensis mixtures of polypeptides that molecular weight is less than 2500Da.
8) take the Cordyceps militaris soya bean solid culture that peanut is auxiliary material
Cultured Cordyceps militaris is expanded to seed liquor, by 10～25%(v/w) inoculum concentration receive after sterilizing on cooling good soya bean, at 22 ℃, cultivated after 5～8 days in darkroom, then bright chamber cultivation 3～6 days; The brightness of described bright chamber is exactly 16 watts of the incandescent lamp power that arrange in IS-RDU3 model isothermal vibration device.In the incubation of bright chamber, suitably stir.
8, dry
Cordyceps militaris soya bean after fermentation ends, is yellow～orange-yellow, with natural wind, dries in the shade, and obtains Cordyceps militaris soya bean finished product.
Cordyceps militaris soya bean prepared by the present invention improves immunity and antioxidant effect detects:
Mouse respectively feeding contains 0(basal diet), the daily ration of 3%, 6%, 12% Cordyceps militaris soya bean (embodiment 2 preparation) and 12% common soya bean, with MDA content in mice plasma and liver, CAT, GSH-Px, T-SOD vigor and inhibition hydroxyl radicals are index, study antioxidation activity (injected in mice D-galactolipin is set up aging model) in its body; Measure immunoglobulin G, M content and cell factor interleukin-4, gamma interferon content in blood plasma and liver organization, Study Mouse immunologic function.Result shows, add Cordyceps militaris soya bean and can significantly improve CAT, GSH-Px in mice plasma and liver, T-SOD (P < 0.05) vigor, the content that suppresses hydroxyl radicals and IgG, IgM, IL-4, IFN-γ (P < 0.05), mouse spleen and liver index be can significantly improve and MDA (P < 0.05) content, the effect that there is delaying senility and improve body's immunity significantly reduced.
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| CN104824588A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis potatoes |
| CN104824574A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps broad beans |
| CN104824728A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis sesames |
| CN105685255A (en) * | 2016-02-22 | 2016-06-22 | 遵义医学院 | Method for manufacturing cordyceps militaris tofu |
| CN108684824A (en) * | 2018-06-12 | 2018-10-23 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean soya-bean milk and preparation method thereof |
| CN108850176A (en) * | 2018-06-12 | 2018-11-23 | 徐州工程学院 | A kind of Cordyceps militaris spawn fermentation soybean soymilk and preparation method thereof |
| CN108841904A (en) * | 2018-07-20 | 2018-11-20 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean peptide and preparation method thereof |
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| CN102274271B (en) * | 2010-06-13 | 2013-08-14 | 深圳市每人康生物科技有限公司 | Soybean plantworms mycelium powder and preparation method thereof |
| CN102334678B (en) * | 2011-07-11 | 2013-03-06 | 范根生 | Method for producing cordyceps mycelium powder by utilizing sprouted soybeans |
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Inventor after: Pu Meizi Inventor after: Wang Ying Inventor after: Wang Fengwu Inventor after: Jin Yulan Inventor before: Pu Meizi Inventor before: Wang Fengwu Inventor before: Jin Yulan |
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