CN101986879B - Manufacturing method for enzyme-containing healthy food and healthy food - Google Patents

Manufacturing method for enzyme-containing healthy food and healthy food Download PDF

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CN101986879B
CN101986879B CN2009101596726A CN200910159672A CN101986879B CN 101986879 B CN101986879 B CN 101986879B CN 2009101596726 A CN2009101596726 A CN 2009101596726A CN 200910159672 A CN200910159672 A CN 200910159672A CN 101986879 B CN101986879 B CN 101986879B
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healthy food
fermentation
weeds
enzyme
zymotic fluid
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CN101986879A (en
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近藤尧
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MIYATOU YASOU KENKYUJO CO Ltd
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MIYATOU YASOU KENKYUJO CO Ltd
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Abstract

The invention provides a manufacturing method of enzyme-containing healthy food. Various functions of the active ingredients such as enzymes, coenzymes, mineral nutrients, vitamins and organic acids like amino acid contained in weeds, vegetables, fruits, kelp and fermentation calcium can be obtained by eating the healthy food. The manufacturing method of the enzyme-containing healthy food is characterized by comprising the following steps: adding sugar, weeds, vegetable fermentation liquid, fruit fermentation liquid, kelp fermentation liquid, fermentation calcium and brown sugar bacteria into the weeds extract to be fermented so as to obtain a first charge liquid; then adding pawpaw fermentation liquid into the first charge liquid to be fermented and cured so as to obtain the second charge liquid; and finally filtering the second charge liquid.

Description

The manufacture method and the healthy food that contain the healthy food of enzyme
Technical field
The present invention relates to contain manufacture method and the healthy food of the healthy food of enzyme.
Background technology
In recent years, because all essential factors such as the picked-up of the height of processed food, high-fat food, vegetables insufficiency of intake and various pressure, the situation that the modern need to promote digestive function gets more and more.
In addition, digestive organs to the picked-up food digest, when absorbing, the various digestive ferments of body endocrine (protein decomposition enzyme, carbohydrate-splitting enzyme, lipolytic enzyme etc.), usually, along with the increase at age, metabolic capability descends, therefore, the synthesis capability of this digestive ferment also descends, the digestion of the food of picked-up, absorption difficult, the contained nutritional labeling of food can not effectively play a role, and it is liable to illness etc. that the result becomes.So, it is believed that the food that especially needs now with advancing age picked-up to contain to help the useful component that digests.
Therefore, as the healthy food that contains enzyme etc. with effect as described above, the inventor has proposed for example No. 3370302 disclosed manufacture method (hereinafter referred to as conventional example) of having used the healthy food of pawpaw of Japan's special permission.
This conventional example relates to a kind of healthy food, it is by extracting the contained extract that is conducive to health of pawpaw, through saccharomycete, be attached to that the fermentation slaking of natural saccharomycete on the pawpaw and lactic acid bacteria obtains contain the healthy food that is conducive to healthy microorganism, enzyme etc., the various enzymes of its convenient picked-up, useful bacterium etc.
Patent documentation 1: No. 3370302 communique of Japan's special permission
Summary of the invention
The inventor makes further research exploitation to conventional example, a kind of manufacture method and healthy food that contains the healthy food of enzyme is provided, wherein, to various weeds, vegetables and fruit ferment, make weeds, the plurality of enzymes that vegetables and fruit are contained, coenzyme, vitamin, amino acid, organic acid, the various active ingredients such as mineral nutrient are in the interior absorbable state of body and form the liquid state that is ingested easily, by taking this healthy food, can obtain admirably for example using simply the weeds of a large amount of lifes, vegetables and fruit can not obtain by described enzyme, coenzyme, vitamin, amino acid, organic acid, the effect that the active ingredients such as mineral nutrient are brought.
Below, with reference to accompanying drawing main points of the present invention are described.
The solution of the present invention 1 relates to a kind of manufacture method that contains the healthy food of enzyme, it is characterized in that, weeds is boiled carry, in the weeds extract of the temperature that can keep enzymatic activity, add sugar, weeds, fermented vegetable liquid, fruit zymotic fluid, sea-tangle zymotic fluid, fermentation calcium and brown sugar bacterium, make its fermentation, obtain the 1st and add feed liquid, then, add adding pawpaw zymotic fluid in the feed liquid to the 1st, make its fermentation, slaking, obtain the 2nd and add feed liquid, then, add feed liquid to the 2nd and filter.
In addition, the solution of the present invention 2 is such as the scheme 1 described manufacture method that contains the healthy food of enzyme, it is characterized in that, described weeds extract is mixed with felon herb raffinate, described fermented vegetable liquid is to add saccharomycete in the extract that extracts to the sugaring of warp-wise vegetables its fermentation is obtained, described fruit zymotic fluid is to add saccharomycete in the extract that extracts to the sugaring of warp-wise fruit its fermentation is obtained, described sea-tangle zymotic fluid is to add saccharomycete in the extract that extracts to the sugaring of warp-wise sea-tangle its fermentation is obtained, described fermentation calcium is with to vegetables, fruit, sea grass, sugaring and make its fermentation and the extract and the shellfish fossil that obtain in the leaf of cereal or plant, oyster shell, fish-bone or pig bone mix, make its fermentation, after the drying, obtain through pulverizing, described pawpaw zymotic fluid is to add saccharomycete in the extract that extracts to the sugaring of warp-wise pawpaw and lactic acid bacteria obtains its fermentation.
In addition, the solution of the present invention 3 is characterized in that for such as the scheme 2 described manufacture methods that contain the healthy food of enzyme, is added with charcoal in the described pawpaw zymotic fluid.
In addition, the solution of the present invention 4 is characterized in that for such as the scheme 3 described manufacture methods that contain the healthy food of enzyme described charcoal is the weeds charcoal.
In addition, the solution of the present invention 5 is characterized in that for such as the scheme 1 described manufacture method that contains the healthy food of enzyme the described the 1st adds feed liquid contains the aloe zymotic fluid.
In addition, the solution of the present invention 6 relates to a kind of healthy food, and it is the healthy food that the scheme 1 described manufacture method that contains the healthy food of enzyme is made, and it is characterized in that the pol of described healthy food is more than 45 degree.
In addition, the solution of the present invention 7 relates to a kind of healthy food, and it is the healthy food that the scheme 2 described manufacture methods that contain the healthy food of enzyme are made, and it is characterized in that the pol of described healthy food is more than 45 degree.
In addition, the solution of the present invention 8 relates to a kind of healthy food, and it is the healthy food that the scheme 3 described manufacture methods that contain the healthy food of enzyme are made, and it is characterized in that the pol of described healthy food is more than 45 degree.
In addition, the solution of the present invention 9 relates to a kind of healthy food, and it is the healthy food that the scheme 4 described manufacture methods that contain the healthy food of enzyme are made, and it is characterized in that the pol of described healthy food is more than 45 degree.
In addition, the solution of the present invention 10 relates to a kind of healthy food, and it is the healthy food that the scheme 5 described manufacture methods that contain the healthy food of enzyme are made, and it is characterized in that the pol of described healthy food is more than 45 degree.
So, the active ingredients such as the contained enzyme of weeds, vegetables, fruit, sea-tangle and fermentation calcium, coenzyme, vitamin, amino acid, organic acid, mineral nutrient become and are very easy in vivo absorbed liquid state, so, the present invention can promote the function of the various excellences of described active ingredient, namely promote digestion, nutrient absorption of food etc., for example immunity increases, and physique improves, and can prevent sick.
Description of drawings
Fig. 1 is the figure of the manufacturing process of explanation present embodiment.
The specific embodiment
At first, the weeds such as sealwort, gynostemma pentaphylla are boiled in hot water, from weeds, extract the mineral nutrient composition, after obtaining the weeds extract, reducing this extraction has the temperature of the weeds extract of mineral nutrient composition, is formed on the later various endonuclease capables of subsequent handling and keeps active state, adds sugar, weeds, fermented vegetable liquid, fruit zymotic fluid, sea-tangle zymotic fluid, fermentation calcium and brown sugar bacterium in this weeds extract, make its fermentation, obtain containing the 1st of various active ingredients and add feed liquid.
In addition, described sugar is the upper white sugar of Japan, brown sugar, compound sugar etc., is used for the pol that raising the 1st adds feed liquid, is the zymogenic nutrient sources such as brown sugar bacterium.
In addition, described weeds is the weeds such as gynostemma pentaphylla, hypericum erectum, the natural Yeast Propagation attached thereto ferment of setting out originally, and the mineral nutrient composition of this weeds also can be extracted.
In addition, described fermented vegetable liquid is the following liquid component that obtains: add sugar in the living vegetables such as bean sprouts, cucumber, mix, osmotic pressure is changed, after elite in the cell of vegetables being extracted the extracellular of vegetables, add saccharomycete, brown sugar bacterium, make its fermentation, by pressurization, separate obtaining described liquid component.That is, it is to make living fermented vegetable and form liquid condition, removes the fermented vegetable liquid that only contains the active ingredient of high concentration behind the residue.
In addition, described fruit zymotic fluid is the following liquid component that obtains: add sugar in the living fruit such as pineapple, apple, mix, osmotic pressure is changed, after elite in the cell of fruit being extracted the extracellular of fruit, add saccharomycete, brown sugar bacterium, make its fermentation, by pressurization, separate obtaining described liquid component.That is, it is to make the fermentation of living fruit and form liquid condition, removes the fruit zymotic fluid that only contains the active ingredient of high concentration behind the residue.
The fermented vegetable liquid that so obtains and fruit zymotic fluid are at the zymotic fluid without fermentation under heating, so a small amount of zymotic fluid just contains many especially enzymes, coenzyme etc.Namely, the contained enzyme of vegetables, fruit, coenzyme etc. can be destroyed because of heating, so do not carry out such heat treated among the present invention, but adopt the method for in the situation of destructive enzyme not, extracting enzyme with the form of zymotic fluid, thus, for resulting liquid, in a small amount of liquid, just contain a large amount of enzymes, coenzyme etc.
In addition, described sea-tangle zymotic fluid is the following liquid component that obtains: add sugar in sea-tangle, mix, osmotic pressure is changed, the elite in the cell of sea-tangle is extracted the extracellular of sea-tangle, then add saccharomycete and make its fermentation, by pressurization, separate obtaining described liquid component.This sea-tangle zymotic fluid contains the active ingredients such as the contained mineral nutrient of a large amount of sea-tangles itself, vitamin, iodine.
In addition, described fermentation calcium obtains by the following method: the middle sugar that add such as leaf to vegetables, fruit, sea grass, cereal or plant make its fermentation, the extract that obtains is mixed with shellfish fossil, oyster shell, fish-bone or pig bone etc., ferment, after the drying, obtain the calcium that ferments through pulverizing, it is water-soluble organic calcium agent, and it is delicious, and absorbability in vivo is very good.
In addition, the brown sugar bacterium is the zymophyte of hobby brown sugar, has the character of fermenting first than other zymophyte.
So, the 1st adds in the feed liquid, the described sugar of dissolving improves the 1st pol that adds in the feed liquid, and become the nutrient source of described brown sugar bacterium, the brown sugar bacterium begins fermentation at first, take the fermentation of this brown sugar bacterium as opportunity, originally be attached to weeds, the saccharomycete of the nature on vegetables and the fruit, lactic acid bacteria, der Pilz, actinomyces, the useful bacterium fermentation such as soil bacteria, breeding, the brown sugar bacterium of these breedings, saccharomycete, lactic acid bacterias etc. make the weeds extract, weeds, fermented vegetable liquid, the fruit zymotic fluid, the sea-tangle zymotic fluid, the fermentations such as fermentation calcium, described enzyme, coenzyme, amino acid, organic acid, the active ingredients such as mineral nutrient are extracted.
Then, add in the feed liquid to the 1st and to add the pawpaw zymotic fluid, make its fermentation, slaking, obtain thus the 2nd and add feed liquid.
Described pawpaw zymotic fluid is following obtaining: add sugar in pawpaw, extraction obtains extract, utilizes the natural saccharomycete that adheres on saccharomycete, lactic acid bacteria, the pawpaw to make the extract fermentation, behind the separating liquid composition, further make this liquid component fermentation, obtain thus described pawpaw zymotic fluid.In addition, this pawpaw contains papain and diversified enzyme (catalase, amylase, lipase, protease, trehalase etc.), amino acid (various must amino acid), vitamin (vitamin C, vitamin E, beta carotene etc.), mineral nutrient (various must mineral nutrient), other nutrient for plants (catechin, flavonoids etc.).
The 2nd adds in the feed liquid, the described the 1st adds the mixed and abundant fusion of feed liquid and pawpaw zymotic fluid, the useful bacterium such as natural saccharomycete that are attached on the pawpaw that the pawpaw zymotic fluid is contained are fermented, the useful bacterium such as the natural saccharomycete of these breedings further makes the fermentations such as weeds extract, weeds, fermented vegetable liquid, fruit zymotic fluid, sea-tangle zymotic fluid, fermentation calcium, and described active ingredient becomes and is absorbed easily in vivo.
So, when absorbing this pawpaw zymotic fluid, the effect of the balance of intestinal bacterium is adjusted in the performance of pawpaw zymotic fluid, namely adjust the balance of beneficial bacteria (for example lactic acid bacteria) and harmful bacteria (for example Bacillus perfringens), the wriggling of intestines is active, the better digested absorption of described active ingredient.
Then, add from the 2nd and to remove weeds the feed liquid, obtain after filtration containing the healthy food of enzyme.
The healthy food of so making is beverage, only can help thus the effect of the digestive ferment in the body to supply in the body by just drinking, the digestion of promotion food, nutrient absorption etc., so for example immunity increases, physique improves, and can prevent sick.
The manufacture method of the healthy food that contains enzyme that the present invention relates to operates as described above, thereby the described healthy food that contains enzyme can make the people absorb simply and effectively as active ingredients such as the neccessary composition enzyme of health and coenzyme, mineral nutrient class, vitamins, amino acid, organic acids.
Embodiment
The below describes embodiments of the invention based on Fig. 1.
Present embodiment is to comprise that the 1st manufacturing process, the 2nd that adds feed liquid adds the manufacture method of the healthy food that contains enzyme of the manufacturing process of feed liquid, these 3 operations of filter progress.Below, each operation is elaborated.
1 the 1st adds the manufacturing process of feed liquid
(1) in the weeds extract, adds sugar, weeds, fermented vegetable liquid, fruit zymotic fluid, sea-tangle zymotic fluid, fermentation calcium and brown sugar bacterium, make its fermentation, obtain the 1st and add feed liquid.
Specifically, with weeds, in advance the fermented vegetable liquid by other operation manufacturings and fruit zymotic fluid, in advance by the aloe zymotic fluid of other operation manufacturings, in advance by the sea-tangle zymotic fluid of other operation manufacturings, put in the tank by the fermentation calcium of other operation manufacturings in advance, the mixed liquor that obtains with adding sugar in weeds extract (adding in this weeds extract has the felon herb raffinate of making by other operations in advance) mixes, add the brown sugar bacterium and make its fermentation, obtain the 1st and add feed liquid.
A is added with the weeds extract of felon herb raffinate
At first the preparation method of weeds extract described.
Weeds is carried out hot water extracting, obtain extract.
The weeds that specifically, will have effective component is put in the still.As weeds, adopt the weeds such as dry sealwort, gynostemma pentaphylla (for example suitable the mixing, adding up to 54.105kg), it is put in 2 stills equably.Described weeds contains a large amount of mineral nutrient compositions.
In addition, weeds is except sealwort, outside the gynostemma pentaphylla, can adopt Japanese wild paulownia, red heart lamb's-quarters (Chenopodium album L var.centroubrum Mak), ginkgo leaf, turmeric, slender acanthopanax, Asiatic plantain, hypericum erectum, sensitive plant Cassia tora (Cassia mimosoides L..subsp.nomame (Siebold) H.Ohashi), Longtube Ground Ivy Herb, agar, folium lycii, the fruit of Chinese wolfberry, Indocalamus leaf (Sasaveitchii (Carr.) Rehd.), cassia bark, wilsonii, meadow pine, dandelion (Taraxacum officinale), dayflower, New Zealand spinach, cordate houttuynia, Nan Tian's leaf, honeysuckle, Cassia occidentalis (Cassia occidentalis L..), Job's tears, cassia seed, the mountain coral, grifola frondosus, silvenine, sweet potato, mushroom, sword bean, the sword bean stem, the sword bean leaf, the fragrant-flowered garlic stem, the fragrant-flowered garlic kind, white oak and argy wormwood etc., and, be not limited to weeds, so long as the medicative plant of tool gets final product, can also adopt mushroom, vegetables, cereal etc.
In addition, when adopting dry weeds as weeds, compare with the weeds that gives birth to, its volume only be about 1/10th, thus process easily, and, which type of can use the various weeds of multiple types season regardless of.
Then, in 2 stills, drop into water (for example each still is equal 300 liters), heat.
Then, (100 ℃) heating is 1 hour after the boiling, boils and carries.
Then, the leach cooking liquid that obtains is taken off liquid with net, be separated into solid constituent (for example 200kg) and extract (for example adding up to 300~320 liters).
Then, the extract that obtains is put in the still, carried out liquid level air-supply with ventilating fan, 100 ℃ of heating about 1.5 hours (for example until liquid measure reach about 215 liters), it is stand-by to make concentrate simultaneously.
Carry by described boiling, extracted the contained mineral nutrient composition (weeds concentrate) of weeds, further mix with the felon herb raffinate of making by other operations described later, obtain the weeds extract.
Then, the preparation method of felon herb raffinate described.
Powder argy wormwood (for example 4kg) is put in other stills, in this still, dropped into water (for example 50 liters), heat stand-by after 10 minutes.
Making felon herb raffinates by other operations is because what use is powder argy wormwood rather than dry argy wormwood.For the powder argy wormwood, boil when carrying, active ingredient just was extracted in the short time, but other weeds is boiled when carrying with pulverous argy wormwood, the viscosity that is mixed with the liquid of this powder argy wormwood increases, and is difficult to be extracted in the hot water so become from other the composition of weeds.Therefore, if when using dry argy wormwood, certainly can carry out described processing with other weeds.
B sugar
As sugar, adopt the upper white sugar of Japan, brown sugar, compound sugar etc.
Specifically, white sugar in the Japan (for example 270kg) is in the effect of the breeding of performance inhibition miscellaneous bacteria, or zymogenic nutrient source.In addition, brown sugar (for example 5kg) is the nutrient source of brown sugar bacterium hobby, and compound sugar (for example 5kg) is the nutrient source of lactic acid bacteria hobby.
The c weeds
Suitably adopt the dry weeds with effective component.
Specifically, as the weeds with effective component, adopt (for example suitable mixing such as gynostemma pentaphylla, hypericum erectum, sensitive plant Cassia tora, folium lycii, Indocalamus leaf, New Zealand spinach, pine needle, garlic, add up to 2.3kg), but so long as contain the weeds of the useful bacterium such as saccharomycete of nature, be not limited to these examples.
Gynostemma pentaphylla has the Ginkgo Biloba function, promotes endocrine, spiritual stabilization, particularly has the effect of the secretion that promotes cortin of succenturiate kidney.
In addition, hypericum erectum has the effect that increases chemical substance dopamine, serotonin, norepinephrine in the brain, and is effective for depression, insomnia.
In addition, the sensitive plant Cassia tora is effective aspect strong, diuresis, antibechic, beriberi, ephritis, jaundice, antimigraine, chronic constipation, yctalopia.
In addition, folium lycii has the effect that the liver function of making is normal, adjust blood pressure, has heart disease, ephrosis, arteriosclerotic preventive effect, easily catch a cold, effective aspect the constipation, heating.
In addition, Indocalamus leaf has antioxidation, contains chlorophyll and the bamfolin (bamfolin) of a large amount of raising immunity, has the effect that forms the physique that is difficult for trouble cancer, lifestyle disease.
In addition, New Zealand spinach contains vitamin, mineral nutrient, chlorophyll, and the contained mucilaginous substance of leaf has the effect of protection mucous membrane.
In addition, pine needle contains vitamin, terpenes, polysaccharide complex, mineral nutrient, catechin, chlorophyll, has the effect of the transmitter substance of softening blood vessel, enhancing brain.
In addition, garlic contains and increases spermin (scordinin), allicin, have the nutrient burning that makes in the body and be converted into energy effect, improve the effect of the vitamin B1 that playing the effect of setting up effect, improve the effect of stomach and intestine activity.
In addition, described weeds also contains the useful bacterium such as saccharomycete of a large amount of natures that originally adheres on weeds.
D fermented vegetable liquid and fruit zymotic fluid
In the present embodiment, do not carry out respectively the manufacturing of fermented vegetable liquid and fruit zymotic fluid, but vegetables and fruit are mixed together, make simultaneously fermented vegetable liquid and fruit zymotic fluid.In addition, in the present embodiment, use the operation different from the operation of fermented vegetable liquid and fruit zymotic fluid to make aloe zymotic fluid described later, but also aloe zymotic fluid and fermented vegetable liquid and fruit zymotic fluid can be made simultaneously.Following fermented vegetable liquid and the fruit zymotic fluid of obtaining: the vegetables that give birth to and the fruit of giving birth to are cleaned, then carry out Subdividing Processing, then add sugar, add saccharomycete, brown sugar bacterium after mixing, stirred once, fermented about 10~14 days in one day, make the useful bacterium breedings such as natural saccharomycete of originally adhering on this saccharomycete, brown sugar bacterium, vegetables and the fruit, then by pressurization, the separating liquid composition obtains described zymotic fluid.
Specifically, the fruit (for example suitable the mixing, adding up to 90kg) that adopts vegetables that bean sprouts, cucumber, spinach etc. give birth to and pineapple, apple, lemon etc. to give birth to, but so long as contain vegetables or the fruit of enzyme and get final product, be not limited to these examples.
These vegetables and fruit are cleaned, then dice or thinly slice after vegetables and fruit are put in the container, then, drop into the upper white sugar (for example 90kg) of Japan as sugar, osmotic pressure is changed, the elite in the cell of vegetables and fruit is extracted outside vegetables and the fresh fruit cell.
Contain diversified enzyme, amino acid, vitamin, mineral nutrient, other plant nutrient (carrotene etc.) in the said extracted thing.The contained main composition of each extract is as follows.
Bean sprouts: amylase, vitamin C, aspartic acid
Cucumber: ascorbinase, vitamin, mineral nutrient, isoquercitrin
Spinach: nitrate reductase, carrotene, vitamin, mineral nutrient
Cabbage: peroxidase, cabbage chitinase, diastase, vitamin, vitamin
Unit green onion: maltose, cholesterol biosynthesis blocking-up enzyme, insulin (グ Le コ キ ニ Application), two propylene sulphur, quercitin
Parsley: ascorbic acid peroxidase, mineral nutrient, vitamin
Potato: mineral nutrient, vitamin C, polyphenyl phenol chlorogenic acid
Ginseng: ascorbinase, carrotene, calcium, food fiber
Cabbage: myrosin, sulforaphane, vitamin C, carrotene, vitamin B2, potassium, calcium
Burdock: peroxidase, inulin
Lotus root: catalase, peroxidase, vitamin, food fiber, mucoprotein
Xiao Song dish: diastase, calcium, vitamin
Turnip: phloroglucin oxidizing ferment, amylase, vitamin C, diastase
Tomato: interior polygalacturonase, ascorbinase, vitamin, potassium, lycopene (リ コ ピ Application)
Radish: diastase, amylase, vitamin, carrotene, calcium
Pineapple: bromelain, saccharic, vitamin
Apple: malic dehydrogenase, malic acid, citric acid, pectin
Lemon: vitamin C, citric acid
In addition, when adopting the upper white sugar (for example 90kg) of Japan as sugar, it is when performance suppresses the effect of breeding of miscellaneous bacteria, or the nutrient source during the zymophyte fermentation.
Then, drop into saccharomycete (for example 200 milliliters) and the brown sugar bacterium (for example 2 liters) such as wine yeast, make the useful bacterium such as natural saccharomycete that originally adhere on vegetables and the fruit ferment, breed about 10~14 days.
At this moment, stirred about 30 minutes with hand once a day, avoid injuring saccharomycete, brown sugar bacterium during stirring, therefore fermentation is carried out well.In addition, by this fermentation, the useful bacterium propagation such as the saccharomycete such as wine yeast, brown sugar bacterium, natural saccharomycete.
Then, pressurize, be separated into residue composition and fermented vegetable liquid and fruit zymotic fluid (for example adding up to about 125 liters).
The fermented vegetable liquid that so obtains and fruit zymotic fluid ferment without heating and obtain, so a small amount of zymotic fluid just contains many especially enzymes.
That is, the vegetables that give birth to as mentioned above, the enzyme that fruit contains can be heated and destroy, but in the present embodiment, the method of enzyme is extracted in employing with the form of zymotic fluid, wherein do not carry out this heat treated, enzyme is damaged, therefore just contain a large amount of enzymes in a small amount of liquid.
E aloe zymotic fluid
Aloe is cleaned, after the Subdividing Processing, added sugar and saccharomycete, stir once every day, carries out the fermentation about 10~14 days, makes the useful bacterium breedings such as natural saccharomycete of originally adhering on this saccharomycete, the aloe, then by pressurization, the separating liquid composition obtains this aloe zymotic fluid thus.
Specifically, the aloe (for example adding up to 90kg) that gives birth to is cleaned, then dice or thinly slice after put in the container.
Then, drop into the upper white sugar (for example 90kg) of Japan as carbohydrate, osmotic pressure is changed, the elite in the cell of aloe is extracted the aloe extracellular.When using the upper white sugar (for example 90kg) of Japan as sugar, the nutrient when it is fermentation, and, because it is refined sugar, so also have the effect of the breeding that suppresses miscellaneous bacteria.
Then, drop into the saccharomycete (for example 100 milliliters) such as wine yeast, fermented about 10~14 days, the useful bacterium breeding such as the natural saccharomycete that originally adheres on this saccharomycete and the aloe.At this moment, stir about one time 30 minute with hand every day, avoids injuring saccharomycete during stirring.
Then, pressurize, the separating liquid composition obtains aloe zymotic fluid (for example adding up to about 130 liters) thus.
This aloe zymotic fluid contains the digestive ferments such as oxidizing ferment (oxidizing ferment), catalase (hydrogen peroxide decomposes enzyme), amylase (amylolytic enzyme), lipase (lipid catabolic enzyme), necessary amino acid, aloin, aloe-emodin, aloe Arbutin etc., this aloe zymotic fluid ferments without heating and obtains, so a small amount of zymotic fluid just contains many especially enzymes.That is, as mentioned above, the vegetables that give birth to, the enzyme that fruit contains can fall because heating is destroyed, and in the present embodiment, adopt the method for extracting enzyme with the form of zymotic fluid, wherein do not carry out this heat treated, enzyme is damaged, therefore just contains a large amount of enzymes in a small amount of liquid.
F sea-tangle zymotic fluid
Sea-tangle is cleaned, after the Subdividing Processing, added sugar and saccharomycete, stir once every day, carries out the fermentation about 10~14 days, and the useful bacterium such as natural saccharomycete that originally adhere on this saccharomycete and the sea-tangle are bred together, then, come the separating liquid composition by pressurization, obtain thus this sea-tangle zymotic fluid.
Specifically, sea-tangle (for example 1kg) is cleaned, then, thinly sliced, put in the container.
Then, drop into upward white sugar (for example 1kg) conduct sugar of Japan, drop into water (3 liters), osmotic pressure is changed, the elite in the cell of extraction sea-tangle.
Then, drop into saccharomycete, carry out the fermentation about 10~14 days, the useful bacterium such as natural saccharomycete that originally adhere on this saccharomycete and the sea-tangle are bred together.At this moment, stirred 30 minutes with hand once a day, avoid damaging saccharomycete during stirring.
Then, pressurize, the separating liquid composition has obtained Thallus Laminariae (Thallus Eckloniae) extract (for example 4 liters).
And then, about pol 47~48 degree, make this Thallus Laminariae (Thallus Eckloniae) extract about 2~3 weeks of fermentation about pH2.5~3.5, make the useful bacterium breeding such as saccharomycete and natural saccharomycete.
This sea-tangle zymotic fluid balancedly contains mineral nutrient, vitamin, iodine and the food fiber that abundant sea-tangle contains itself.
The g calcium that ferments
Fermentation calcium is following water-soluble organic calcium agent of making by following 3 procedures (technology that No. the 3308475th, Japan's special permission): the first operation, in the leaf of vegetables, fruit, sea grass, cereal or plant etc., add carbohydrate, making pol is 50~55 degree, make its fermentation, this fermentate is filtered, make the extract slurry; The second operation, the extract slurry that shellfish fossil, oyster shell, fish-bone, pig bone and described the first operation are made mixes, make the reaction of the contained inorganic calcium such as described shellfish fossil, described oyster shell, described fish-bone, described pig bone and described extract slurry, fermentation, make described inorganic calcium become organic calcium; The 3rd operation makes this reaction fermentate dry, then, pulverizes, and makes powder.
The concrete 100g calcium that should ferment that uses.
This fermentation calcium is delicious and the interior extraordinary fermentation calcium of absorbability of body.
H brown sugar bacterium
The brown sugar bacterium is the zymophyte of hobby brown sugar, has the character of fermenting first than other zymophyte.
(2) mixed processes of above-mentioned a~h
At first, in " felon herb raffinate " of a, add " brown sugar (for example 5kg) and the compound sugar (for example 5kg) " that mixes b.In addition, in " the weeds concentrate " of a, add " white sugar in the Japan (for example 270kg) " that mixes b.
Above-mentioned felon herb raffinate and weeds concentrate are put in the tank.At this moment, the liquid temperature of felon herb raffinate and weeds concentrate is controlled at below 48 ℃.This is because can not destroy like this enzyme that is added by each liquid in follow-up operation.
In addition, put in the tank after felon herb raffinate and weeds concentrate can also be pre-mixed.
Then, drop into " dry weeds ", " fermented vegetable liquid and fruit zymotic fluid ", " aloe zymotic fluid ", " sea-tangle zymotic fluid ", " the fermentation calcium " of g of f of e of d of c in the tank, after the mixing, " the brown sugar bacterium " that further adds h, stir with hand, placed 10~14 days in 20~27 ℃ of room temperatures, humidity 40~60%, then, placed about 1.5 months.At this moment, stir once a day about 5 minutes, promote fermentation, obtain the 1st and add feed liquid.
The 1st adds in the feed liquid, and the sugar of described b is not put in the felon herb raffinate that heats, the weeds concentrate that heats, so dissolve well.
2 the 2nd add the manufacturing process of feed liquid
(1) adds in the feed liquid to the described the 1st and add the pawpaw zymotic fluid, make its fermentation, slaking, obtain the 2nd and add feed liquid.
Specifically, in the pawpaw zymotic fluid of making by other operations in advance, mix the weeds charcoal of making by other operations in advance, to put into the 1st through the pawpaw zymotic fluid that contains the weeds charcoal that fermented 30 days and add in the feed liquid, make its fermentation, slaking 5~6 months, and obtain the 2nd and add feed liquid.
A contains the pawpaw zymotic fluid of weeds
In the pawpaw zymotic fluid, add the weeds charcoal through pulverizing, after the mixing, make its fermentation, slaking stand-by after 30 days.
(a) pawpaw zymotic fluid
This pawpaw zymotic fluid is following obtaining: after the pawpaw Subdividing Processing, add carbohydrate, utilize osmotic pressure, extract the elite in the pawpaw cell, in this extract, add saccharomycete, the lactic acid bacterias such as wine yeast, more than stirring every day once, ferment 10~14 days, make the natural yeast propagation that script adheres on described saccharomycete, lactic acid bacteria and the pawpaw after, by pressurization, the separating liquid composition further makes this liquid component fermentation, slaking 30 days, obtains thus this pawpaw zymotic fluid.
Specifically, it is made by following operation.
As the raw material pawpaw, adopt Japan to produce pawpaw.Other countries export to the pawpaw of Japan when having used a large amount of anticorrisive agents, pesticide, and these anticorrisive agents, pesticide are undesirable for making healthy food.
In addition, the preferred feedstock pawpaw is without agricultural chemicals.
This pawpaw (80kg) is cleaned, then, after dicing or thinly slicing, put in the tank, then, drop into the upper white sugar (80kg) of Japan, osmotic pressure is changed, extract the elite in the pawpaw cell.
Need to prove, contain papain and diversified enzyme (catalase, amylase, lipase, protease, trehalase etc.), amino acid (various necessary amino acid), vitamin (vitamin C, vitamin E, beta carotene etc.), mineral nutrient (various necessary mineral nutrient), other plant nutrient (catechin, flavonoids etc.) in the said extracted thing.
In addition, the segmentation compound of pawpaw and carbohydrate can little by little replace interpolation, apply as much as possible, equably osmotic pressure to the pawpaw cell.
In addition, when adopting the upper white sugar of Japan as carbohydrate, also has the effect of the breeding that suppresses miscellaneous bacteria in the time of nutrient when carbohydrate is the zymophyte fermentation.
Then, drop into saccharomycete (200 milliliters) and lactic acid bacteria, ferment 10~14 day more than stirring once with hand every day, makes the natural saccharomycete breeding of originally adhering on saccharomycete, lactic acid bacteria and the pawpaw.By this fermentation, breeding, the natural saccharomycete that originally adheres on saccharomycete and lactic acid bacteria and the pawpaw merges.
It is different because of equipment etc. to carry out period that symbiosis cultivates, has about 70% to be extracted to pawpaw extracellular period but be set as elite in the pawpaw cell.
In addition, alleged saccharomycete refers to the common saccharomycete such as wine yeast, alcohol yeast bacterium, saccharomyces cerevisiae herein.
Then, by pressurization, residue with after papaya liquid separates, is moved into fermentation tank with this papaya liquid, in time add the saccharomycete such as wine yeast, and mix, it was fermented 30 days.At this moment, when going to steep, use rod to stir one time 15 minutes in one day.
This pawpaw zymotic fluid contains the strong liquid component of SOD (superoxide dismutase) effect, the effect with balance of adjusting intestinal bacterium.
In addition, as the concrete effect of pawpaw zymotic fluid, the decomposition and draining that the propagation of improving (improvement that is Nutrient Cycle), pathogen, harmful bacteria of can enumerate the purification of blood, the activation of cell, sanguimotor improvement, arteriosclerotic prevention, digesting and assimilating metabolism suppresses and infect production increase, harmful substance, the carcinogen of inhibitions, immune activation, hormone and vitamin promotes, the activation of the stablizing of pH in the intestines, activeization that intestines are movable, lipid-metabolism, glycometabolic improvement, hepatic and renal function and save from damage etc.
(b) weeds charcoal
The weeds charcoal obtains (Japanese Patent Application 2005-84105 number technology) by irradiation far infrared when weeds is carried out charing.
In addition, so long as the charcoal that weeds forms gets final product, be not limited to the weeds charcoal of making by said method.
Specifically, described weeds charcoal is the following weeds charcoal of making: when in adding heating kiln, the weeds of drying being heated, obtain the weeds charcoal by the irradiation far infrared, again it is pulverized, and the fine weeds charcoal that obtains.
This weeds can suitably be adopted and is selected from the red heart lamb's-quarters; wild paulownia; gynostemma pentaphylla; ginkgo; slender acanthopanax; turmeric; wilsonii; Asiatic plantain; hypericum erectum; Longtube Ground Ivy Herb; the sensitive plant Cassia tora; Radix Glycyrrhizae; aloe; folium lycii; the fruit of Chinese wolfberry; Indocalamus leaf; cassia bark; cassia seed; meadow pine; dandelion; the mountain coral; dayflower; New Zealand spinach; cordate houttuynia; sealwort; Nan Tian; honeysuckle; Job's tears; Cassia occidentalis; silvenine; sweet potato; argy wormwood; sword bean; grifola frondosus; mushroom; the larch mushroom; the weeds of Phellinus etc.; but be not limited to weeds; so long as the medicative plant of tool gets final product, can also adopt mushroom; vegetables; cereal etc.
In addition, for example can also adopt the 1st to add and boil the residue (contain in this residue and do not extract clean active ingredient) of carrying behind the weeds in the feed liquid preparation.In this case, can effectively utilize so far always weeds residue as discarded object.
(c) above-mentioned (a), the fermentation of (b), curing step
Add " weeds charcoal (for example 0.16kg) " of described (b) in " pawpaw zymotic fluid (for example 20 liters) " of described (a) and mix, placed about 30 days.Make its fermentation, slaking in this placement of about 30 days, obtain containing the pawpaw zymotic fluid of weeds charcoal.
In described about 30 days fermentation, slaking, pawpaw zymotic fluid and weeds charcoal merge well.
In addition, the weeds charcoal of sneaking into fermentation and be decomposed carefullyyer in the usefulness that the adsorptivity that the various usefulness that obtain weeds and the distinctive meticulous adsorption hole of weeds charcoal produce is brought, is difficult for constipation and ediblely feels.
B fermentation, curing step
Add in the feed liquid " the pawpaw zymotic fluid that contains the weeds charcoal " that adds described a and mix to the described the 1st, fermentation, slaking 5~6 months obtain the 2nd and add feed liquid.
The 2nd adds in the feed liquid, by 5~6 months fermentations, slaking, useful bacterium breeds at leisure, not only contain the active ingredients such as a large amount of various enzymes, coenzyme, vitamin, organic acid, mineral nutrient, also contain enzyme SOD with the effect of removing active oxygen etc., so fermentate is stable, be difficult for corrupt.
3 filter progress
Add from the described the 2nd and to remove weeds the feed liquid, after filtration, separation and Extraction filtrate is bottled this filtrate etc., obtains containing the healthy food of enzyme.
The pH of the healthy food that obtains is about 3.7~3.9, and alcoholic degree is about 2.5~4.0%, and final pol is about 45~53 degree, preferred 52~53 degree of pol.
Drink the healthy food that contains enzyme that present embodiment obtains, can obtain following result.
(1) before, at night finger tip is numb active and wakes up with a start, begin to drink this healthy food after, improving situation, (women) can sleep soundly.
(2) nail hardening, general red changes healthy nail into, health progressively take a turn for the better (male sex).
(3) blood pressure stabilization, free movement of the bowels (male sex).
(4) after nearly 1 year the drinking, the polyp of " gall-bladder " disappear (women).
(5) cholesterol value decline (male sex).
(6) begin to drink after, tired, taediumvitae disappear (male sex).
(7) loss of appetite and weight in summer no longer can be tided over summer (women) spiritually.
(8) drink very convenient (women).

Claims (10)

1. manufacture method that contains the healthy food of enzyme, it is characterized in that, the first weeds is boiled carry, in the weeds extract of the temperature that can keep enzymatic activity, add sugar, the second weeds, fermented vegetable liquid, fruit zymotic fluid, sea-tangle zymotic fluid, fermentation calcium and brown sugar bacterium, make its fermentation, obtain the 1st and add feed liquid, then, add adding pawpaw zymotic fluid in the feed liquid to the 1st, make its fermentation, slaking, obtain the 2nd and add feed liquid, then, add feed liquid to the 2nd and filter; Described the first weeds is selected from sealwort, gynostemma pentaphylla, the wild paulownia of Japan, the red heart lamb's-quarters, ginkgo leaf, turmeric, slender acanthopanax, Asiatic plantain, hypericum erectum, the sensitive plant Cassia tora, Longtube Ground Ivy Herb, agar, folium lycii, the fruit of Chinese wolfberry, Indocalamus leaf, cassia bark, wilsonii, meadow pine, dandelion, dayflower, New Zealand spinach, cordate houttuynia, Nan Tian's leaf, honeysuckle, Cassia occidentalis, Job's tears, cassia seed, the mountain coral, grifola frondosus, silvenine, sweet potato, mushroom, sword bean, the sword bean stem, the sword bean leaf, the fragrant-flowered garlic stem, the fragrant-flowered garlic kind, in white oak and the argy wormwood; Described the second weeds is selected from gynostemma pentaphylla, hypericum erectum, sensitive plant Cassia tora, folium lycii, Indocalamus leaf, New Zealand spinach, pine needle, the garlic.
2. the manufacture method that contains the healthy food of enzyme as claimed in claim 1, it is characterized in that, described weeds extract is mixed with felon herb raffinate, described fermented vegetable liquid is sugaring and add saccharomycete in the extract that extracts its fermentation is obtained in the warp-wise vegetables, described fruit zymotic fluid is to add saccharomycete in the extract that extracts to the sugaring of warp-wise fruit its fermentation is obtained, described sea-tangle zymotic fluid is to add saccharomycete in the extract that extracts to the sugaring of warp-wise sea-tangle its fermentation is obtained, described fermentation calcium is with to vegetables, fruit, sea grass, sugaring and make its fermentation and the extract and the shellfish fossil that obtain in the leaf of cereal or plant, oyster shell, fish-bone or pig bone mix, make its fermentation, after the drying, obtain through pulverizing, described pawpaw zymotic fluid is to add saccharomycete in the extract that extracts to the sugaring of warp-wise pawpaw and lactic acid bacteria obtains its fermentation.
3. the manufacture method that contains the healthy food of enzyme as claimed in claim 2 is characterized in that, is added with charcoal in the described pawpaw zymotic fluid.
4. the manufacture method that contains the healthy food of enzyme as claimed in claim 3 is characterized in that, described charcoal is the weeds charcoal.
5. the manufacture method that contains the healthy food of enzyme as claimed in claim 1 is characterized in that, the described the 1st adds feed liquid contains the aloe zymotic fluid.
6. healthy food, it is the healthy food that the manufacture method that contains the healthy food of enzyme claimed in claim 1 is made, and it is characterized in that, the pol of described healthy food is more than 45 degree.
7. healthy food, it is the healthy food that the manufacture method that contains the healthy food of enzyme claimed in claim 2 is made, and it is characterized in that, the pol of described healthy food is more than 45 degree.
8. healthy food, it is the healthy food that the manufacture method that contains the healthy food of enzyme claimed in claim 3 is made, and it is characterized in that, the pol of described healthy food is more than 45 degree.
9. healthy food, it is the healthy food that the manufacture method that contains the healthy food of enzyme claimed in claim 4 is made, and it is characterized in that, the pol of described healthy food is more than 45 degree.
10. healthy food, it is the healthy food that the manufacture method that contains the healthy food of enzyme claimed in claim 5 is made, and it is characterized in that, the pol of described healthy food is more than 45 degree.
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Publication number Priority date Publication date Assignee Title
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CN104223036B (en) * 2013-06-24 2017-07-14 梁岩 Being fermented using composite bacteria improves the method for plant enzyme food local flavor and texture
CN104336547B (en) * 2013-07-30 2021-05-18 杭州立一真生物科技有限公司 Fig enzyme and preparation method thereof
CN104824097A (en) * 2015-04-27 2015-08-12 郭台松 Enzyme, enzyme solution and relevant product
CN104940450A (en) * 2015-05-22 2015-09-30 北京联华大通教育科技有限公司 Health beverage for enhancing body immunity and preparation method thereof
CN105520111A (en) * 2015-12-28 2016-04-27 湖北工业大学 Production method of fermented garlic smell sheep bone soup stock
CN105521178B (en) * 2015-12-29 2019-09-06 海南海神同联生物科技开发有限公司 A kind of soft capsule with aloe and its preparation method and application
CN106072597A (en) * 2016-07-26 2016-11-09 厦门益力康生物科技有限公司 One is replenished the calcium strong bone ferment and preparation method thereof
CN106912764A (en) * 2017-03-08 2017-07-04 南京工业大学 Method for preparing traditional Chinese medicine fruit and vegetable enzyme drink
CN107095942A (en) * 2017-06-23 2017-08-29 戴元强 A kind of biotherapy for utilizing microbial compound preparation to be used for prevention from suffering from the diseases and treatment

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1148495A (en) * 1995-10-23 1997-04-30 安淑荣 Oral liquor of microbe
CN1741750A (en) * 2002-12-20 2006-03-01 科学与工业研究会 Health protective herbal soft drink

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1148495A (en) * 1995-10-23 1997-04-30 安淑荣 Oral liquor of microbe
CN1741750A (en) * 2002-12-20 2006-03-01 科学与工业研究会 Health protective herbal soft drink

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JP特开2001-120224A 2001.05.08

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