CN105521178B - A kind of soft capsule with aloe and its preparation method and application - Google Patents
A kind of soft capsule with aloe and its preparation method and application Download PDFInfo
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- CN105521178B CN105521178B CN201511015993.0A CN201511015993A CN105521178B CN 105521178 B CN105521178 B CN 105521178B CN 201511015993 A CN201511015993 A CN 201511015993A CN 105521178 B CN105521178 B CN 105521178B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/886—Aloeaceae (Aloe family), e.g. aloe vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4816—Wall or shell material
- A61K9/4825—Proteins, e.g. gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention discloses a kind of soft capsule with aloe and its preparation method and application, the filler of the soft capsule with aloe is made of following components according to mass ratio: the quality of vegetable oil is 1.1~1.2 times of aloe quality of fermentation broth, and the quality of suspending agent is 0.1~0.5 times of aloe quality of fermentation broth；The aloe fermentation liquid is made of following components according to mass ratio: red edge intend shelf fungus fermentation liquid quality be 0.5~1 times of aloe full leaf freeze-dried powder quality, 1~2.5 times that the quality of tremella strain fermentation liquid is aloe full leaf freeze-dried powder quality.Pharmacological evaluation proves that the soft capsule with aloe can mitigate thrombotic weight in wet base, can be obviously prolonged mouse bleeding and clotting time, and having improves microcirculation, blood coagulation resisting function, has the function of antithrombotic, facilitates the effect of inhibition thrombosis.
Description
Technical field
The present invention relates to medicines and health protection fields more particularly to a kind of soft capsule with aloe and its preparation method and application.
Background technique
Aloe is Liliaceae perennial evergreen herbaceous plant, and aloe frozen-dried powder uses aloe, super dense through highest science and technology
Contracting mentions composition of selecting the essence, and contains abundant rheum emodin, glycan polysaccharide, protein, ferment, vitamin, microelement, amino acid
Deng.Promote intestinal peristalsis studies have shown that aloe frozen-dried powder has, relax bowel and defecation, improve constipation, deep layer toxin expelling, antiulcer, anti-inflammatory
Removing toxic substances improves immunity, and beautifying whitening skin is crease-resistant, eliminate color spot, promotion skin renewal, alleviation gynecologic menstrual is uncomfortable, promotes
Mammary development, the magical effect for promoting wound healing.
Therefore, the further investigation and exploitation of aloe especially aloe frozen-dried powder, it will more selections are brought to consumer,
Especially for the sub-health population of the hypoimmunities such as cardiovascular patient, the health care product of strengthen immunity is provided, is had
Huge Social benefit and economic benefit possesses good application prospect.
It is universal fungi that red edge, which intends shelf fungus,.Red edge intend shelf fungus in containing there are many three cover class compound and
Other a variety of chemical components.Research shows that the extract that red edge intends shelf fungus has antitumor, antibacterial, anti-inflammatory, anti-oxidant, inhibition
Fat, protection liver, inhibition oedema effect, and free radical can be removed, adjust central nervous system, treatment diabetes, enhancing
Body immunity.
Tremella also known as witches' butter, Tremella mesenterica etc., main producing region are the Lijing in Yunnan, enlightening celebrating, Dali, Gong Shan, Tibet
Etc. ground.Fructification is golden yellow, like brain fold shape.Smell faint scent, food flavor are delicious, are food, medicine dual-purpose bacterium, contain protein, class Hu
Radish element, mucopolysaccharide, collagen, fat, carbohydrate, minerals, vitamins and other nutritious components, and there is distinct chemical
The compound of structure and high degree of symmetry.
In the prior art, red edge intend shelf fungus and tremella be usually used in anti-tumor drug and cardiovascular disease research and
Exploitation intends shelf fungus and tremella for the also very big development space in terms of health care product for red edge.
Summary of the invention
In view of this, the present invention provides a kind of soft capsule with aloe and preparation method thereof.The soft capsule with aloe, which has, to be mentioned
The effect of high immunity, has a good application prospect.
In a first aspect, the present invention provides a kind of soft capsule with aloe, by the filler of aloe fermentation liquid and soft capsule with aloe
It is made, according to mass ratio meter, the filler of the soft capsule with aloe includes following components:
The quality of vegetable oil is 1.1~1.2 times of aloe quality of fermentation broth, and the quality of suspending agent is aloe quality of fermentation broth
0.1~0.5 times；
According to mass ratio meter, the aloe fermentation liquid includes following components:
The quality that red edge intends shelf fungus fermentation liquid is 0.5~1 times, tremella strain fermentation liquid of aloe full leaf freeze-dried powder quality
Quality be 1~2.5 times of aloe full leaf freeze-dried powder quality.
Preferably, the aloe fermentation liquid, vegetable oil and suspending agent mass ratio are 1:1.1:0.2.
Preferably, the aloe full leaf freeze-dried powder, red edge intend shelf fungus fermentation liquid and the quality of tremella strain fermentation liquid is
1:0.5:1.5。
Second aspect, the present invention provides a kind of preparation methods of soft capsule with aloe, include the following steps:
1) red edge is intended into shelf fungus and is inoculated into following culture mediums: potato 250g/L, lactose 20g/L~40g/L, beancake powder 1
~10g/L, 0.5~20g/L of potassium dihydrogen phosphate, 0.5~20g/L of magnesium sulfate, adjusting pH value is 6.5~7.5, is cultivated at 23~28 DEG C
1~2 day, filtering intended shelf fungus fermentation liquid to get red edge；
2) tremella strain is inoculated into following culture mediums: potato 200g/L, corn flour 20g/L~40g/L, glucose 10g/
L~40g/L, peptone 2g/L~10g/L, potassium dihydrogen phosphate 0.2g/L~20g/L, magnesium sulfate 0.15g/L~20g/L, VB
0.01g/L~10g/L, adjusting pH value is 5~7, is cultivated 2~3 days at 24~26 DEG C, is filtered to get tremella strain fermentation liquid；
3) the red edge is intended shelf fungus fermentation liquid, the tremella strain fermentation liquid to mix with aloe full leaf freeze-dried powder,
25 DEG C~30 DEG C ferment 5~7 days, filter, and place, and filter, and sterilizing obtains aloe fermentation liquid；Wherein, red edge intends shelf fungus hair
The quality of zymotic fluid is 0.5~1.5 times of aloe full leaf freeze-dried powder quality, the quality of tremella strain fermentation liquid is aloe full leaf freeze-drying
1~2.5 times of silty amount；
4) vegetable oil and suspending agent Hybrid Heating are stirred, is mixed after cooling with the aloe fermentation liquid, grinding, mistake
Sieve is filled to capsule casing material, and pelleting washes ball, dry, and sizing obtains the soft capsule with aloe；Wherein, the quality of vegetable oil
It is 1.1~1.2 times of aloe quality of fermentation broth, the quality of suspending agent is 0.1~0.5 times of aloe quality of fermentation broth.
Preferably, the aloe fermentation liquid, vegetable oil and suspending agent mass ratio are 1:1.1:0.2.
Preferably, the aloe full leaf freeze-dried powder, red edge intend shelf fungus fermentation liquid and the quality of tremella strain fermentation liquid is
1:0.5:1.5。
The third aspect, the present invention also provides a kind of soft capsule with aloe as described in relation to the first aspect to prepare antithrombotic reagent
In application.
Specific embodiment
Following embodiment is that principles and features of the present invention are described, and illustrated embodiment is served only for explaining the present invention,
It is not intended to limit the scope of the present invention.
Embodiment 1
A kind of preparation method of the soft capsule with aloe of raw material quasi- shelf fungus containing red edge, includes the following steps:
1) red edge is intended into shelf fungus and is inoculated into following culture mediums: potato 250g/L, lactose 20g/L, beancake powder (taking juice) 5g/
L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.5g/L, adjusting pH value is 7, is cultivated 2 days at 23 DEG C, and filtering intends shelf fungus hair to get red edge
Zymotic fluid；
2) the red edge of 1000g is intended shelf fungus fermentation liquid to mix with 1000g aloe full leaf freeze-dried powder, is fermented 6 days at 28 DEG C, mistake
Filter is placed, and is filtered, and sterilizing obtains aloe fermentation liquid；
3) 250g soybean oil is added in water-bath type warm ingredients tank (100L type, Wenzhou Kerui Machinery Co., Ltd.'s production)
Heat to addition 30g beeswax after 60 DEG C stirs 30 minutes, is down to 50 DEG C to temperature and adds aloe fermentation liquid described in 220g, side is added
Side stir about 25 minutes, aloe capsule filler is made；Aloe capsule filler is crossed into colloid mill (JM80 type, Langfang universal machine
The production of tool Manufacturing Co., Ltd), 80 meshes are crossed after stirring 2 hours, puts into stirring 30 minutes in ingredients pot and releases again after sieving, to
With.
Capsule casing material is made of gelatin, glycerol, purified water, sodium copper chlorophyllin；Gelatin: glycerol: purified water ratio be for
1:0.45:1；Sodium copper chlorophyllin dosage is 0.25g/kg.It is operated by the standard practice instructions for changing glue tank, pre- thermalization glue tank
(RJHA-200L type, Wenzhou Rongji Capsule Medicinal Chemical Equipment Co., Ltd.'s production), by process recipe requirement, is added 280g purifying
Water, 126g glycerol are heated to 70 DEG C of dissolutions；280g gelatin is added, stirs 20 minutes, is down to 70 DEG C to temperature, 0.2g leaf is added
Glue is made after five minutes, opens vacuum pump and vacuumizes to glue for green element copper sodium, stirring, and bubble is eliminated to liquid level, is stablized
30 minutes afterwards, vacuum degree 0.07mpa.According to glue is cooked after inspection appearance, viscosity are qualified, glue is put as required, crosses 100
It is banished after mesh in 60 DEG C of heat preservations in glue bucket, for use.
By aloe capsule filler obtained and capsule casing material, by processing parameter, (shape is ellipse, loading amount
500mg/, 55 DEG C of glue box temperature, temperature of charge are room temperature, control loading amount in 500mg ± 37.5mg), in pellet press (RGY6-
20 types, Changzheng Tianmin High Science & Technology Co., Ltd., Beijing production) on by pelleting standard practice instructions operate.
After pelleting semi-finished product by conveyer belt enter rotating cage sizing, setting temperature be 20 DEG C, relative humidity 30% hereinafter, when
Between 3 hours.It is cleaned with 95% edible alcohol, removes the Valelinum Liquidum for being attached to capsule and pill outer surface, repeat to clean to capsule and pill surface
Completely, alcohol is drained.By disk on the capsule and pill after sizing, it is pushed into drying shed and is dried；25 DEG C of drying temperature in drying shed, relatively
Humidity 25%.Drying time 24 hours, obtain the soft capsule with aloe for intending shelf fungus containing red edge.
Embodiment 2
A kind of preparation method of soft capsule with aloe of the raw material containing tremella, includes the following steps:
1) tremella strain is inoculated into following culture mediums: potato 200g/L, corn flour 30g/L, glucose 20g/L, albumen
Peptone 6g/L, potassium dihydrogen phosphate 3g/L, magnesium sulfate 2g/L, VB10.2g/L, adjust pH value be 7,25 DEG C cultivate 3 days, filtering to get
Tremella strain fermentation liquid；
2) 1500g tremella strain fermentation liquid is mixed with 1000g aloe full leaf freeze-dried powder, is fermented 6 days at 28 DEG C, filtering,
It places, filters, sterilizing obtains aloe fermentation liquid；
3) step 3) in the same manner as in Example 1 carries out the preparation of further soft capsule with aloe, is made containing tremella
Soft capsule with aloe.
Embodiment 3
A kind of preparation method of soft capsule with aloe of the raw material containing the quasi- shelf fungus of red edge and tremella, includes the following steps:
1) red edge is intended into shelf fungus and is inoculated into following culture mediums: potato 250g/L, lactose 20g/L, beancake powder 1g/L, phosphoric acid
Potassium dihydrogen 0.5g/L, magnesium sulfate 0.5g/L, adjusting pH value is 6.5, is cultivated 1 day at 23 DEG C, and filtering intends shelf fungus fermentation to get red edge
Liquid；
2) tremella strain is inoculated into following culture mediums: potato 200g/L, corn flour 20g/L, glucose 10g/L, albumen
Peptone 2g/L, potassium dihydrogen phosphate 0.2g/L, magnesium sulfate 0.15g/L, VB 0.01g/L, adjusting pH value is 5, is cultivated 2 days at 24 DEG C, mistake
Filter is to get tremella strain fermentation liquid；
3) red edge described in 500g is intended into tremella strain fermentation liquid and 100g aloe full leaf described in shelf fungus fermentation liquid, 1000g
Freeze-dried powder mixing, ferments 5 days at 25 DEG C, filters, and places, and filters, and sterilizing obtains aloe fermentation liquid；
4) 242g soybean oil is added in water-bath type warm ingredients tank (100L type, Wenzhou Kerui Machinery Co., Ltd.'s production)
Heat to addition 22g beeswax after 60 DEG C stirs 30 minutes, is down to 50 DEG C to temperature and adds aloe fermentation liquid described in 220g, side is added
Side stir about 25 minutes, aloe capsule filler is made；Aloe capsule filler is crossed into colloid mill (JM80 type, Langfang universal machine
The production of tool Manufacturing Co., Ltd), 80 meshes are crossed after stirring 2 hours, puts into stirring 30 minutes in ingredients pot and releases again after sieving, to
With.
Capsule casing material is made of gelatin, glycerol, purified water, sodium copper chlorophyllin；Gelatin: glycerol: purified water ratio be for
1:0.45:1；Sodium copper chlorophyllin dosage is 0.25g/kg.It is operated by the standard practice instructions for changing glue tank, pre- thermalization glue tank
(RJHA-200L type, Wenzhou Rongji Capsule Medicinal Chemical Equipment Co., Ltd.'s production), by process recipe requirement, is added 280g purifying
Water, 126g glycerol are heated to 70 DEG C of dissolutions；280g gelatin is added, stirs 20 minutes, is down to 70 DEG C to temperature, 0.2g leaf is added
Glue is made after five minutes, opens vacuum pump and vacuumizes to glue for green element copper sodium, stirring, and bubble is eliminated to liquid level, is stablized
30 minutes afterwards, vacuum degree 0.07mpa.According to glue is cooked after inspection appearance, viscosity are qualified, glue is put as required, crosses 100
It is banished after mesh in 60 DEG C of heat preservations in glue bucket, for use.
By aloe capsule filler obtained and capsule casing material, by processing parameter, (shape is ellipse, loading amount
500mg/, 55 DEG C of glue box temperature, temperature of charge are room temperature, control loading amount in 500mg ± 37.5mg), in pellet press (RGY6-
20 types, Changzheng Tianmin High Science & Technology Co., Ltd., Beijing production) on by pelleting standard practice instructions operate.
After pelleting semi-finished product by conveyer belt enter rotating cage sizing, setting temperature be 20 DEG C, relative humidity 30% hereinafter, when
Between 3 hours.It is cleaned with 95% edible alcohol, removes the Valelinum Liquidum for being attached to capsule and pill outer surface, repeat to clean to capsule and pill surface
Completely, alcohol is drained.By disk on the capsule and pill after sizing, it is pushed into drying shed and is dried；25 DEG C of drying temperature in drying shed, relatively
Humidity 25%.Drying time 24 hours, obtain the soft capsule with aloe for intending shelf fungus containing red edge.
Embodiment 4
A kind of preparation method of soft capsule with aloe of the raw material containing the quasi- shelf fungus of red edge and tremella, includes the following steps:
1) red edge is intended into shelf fungus and is inoculated into following culture mediums: potato 250g/L, lactose 40g/L, beancake powder 10g/L, phosphorus
Acid dihydride potassium 20g/L, magnesium sulfate 20g/L, adjusting pH value is 7.5, is cultivated 2 days at 28 DEG C, and filtering intends shelf fungus fermentation to get red edge
Liquid；
2) tremella strain is inoculated into following culture mediums: potato 200g/L, corn flour 40g/L, glucose 40g/L, albumen
Peptone 10g/L, potassium dihydrogen phosphate 20g/L, magnesium sulfate 20g/L, VB 10g/L, adjusting pH value is 7, is cultivated 3 days at 26 DEG C, filtering, i.e.,
Obtain tremella strain fermentation liquid；
3) red edge described in 1500g is intended into tremella strain fermentation liquid described in shelf fungus fermentation liquid, 2500g and 1000g aloe is complete
The mixing of leaf freeze-dried powder, ferments 7 days at 30 DEG C, filters, and places, and filters, and sterilizing obtains aloe fermentation liquid；
4) 264g soybean oil is added in water-bath type warm ingredients tank (100L type, Wenzhou Kerui Machinery Co., Ltd.'s production)
Heat to addition 110g beeswax after 60 DEG C stirs 30 minutes, is down to 50 DEG C to temperature and adds aloe fermentation liquid described in 220g, Bian Jia
Enter side stir about 25 minutes, aloe capsule filler is made；Aloe capsule filler is crossed colloid mill, and (JM80 type, Langfang are general
Machinery Manufacturing Co., Ltd.'s production), 80 meshes are crossed after stirring 2 hours, puts into stirring 30 minutes in ingredients pot and releases again after sieving,
For use.
Capsule casing material is made of gelatin, glycerol, purified water, sodium copper chlorophyllin；Gelatin: glycerol: purified water ratio be for
1:0.45:1；Sodium copper chlorophyllin dosage is 0.25g/kg.It is operated by the standard practice instructions for changing glue tank, pre- thermalization glue tank
(RJHA-200L type, Wenzhou Rongji Capsule Medicinal Chemical Equipment Co., Ltd.'s production), by process recipe requirement, is added 280g purifying
Water, 126g glycerol are heated to 70 DEG C of dissolutions；280g gelatin is added, stirs 20 minutes, is down to 70 DEG C to temperature, 0.2g leaf is added
Glue is made after five minutes, opens vacuum pump and vacuumizes to glue for green element copper sodium, stirring, and bubble is eliminated to liquid level, is stablized
30 minutes afterwards, vacuum degree 0.07mpa.According to glue is cooked after inspection appearance, viscosity are qualified, glue is put as required, crosses 100
It is banished after mesh in 60 DEG C of heat preservations in glue bucket, for use.
By aloe capsule filler obtained and capsule casing material, by processing parameter, (shape is ellipse, loading amount
500mg/, 55 DEG C of glue box temperature, temperature of charge are room temperature, control loading amount in 500mg ± 37.5mg), in pellet press (RGY6-
20 types, Changzheng Tianmin High Science & Technology Co., Ltd., Beijing production) on by pelleting standard practice instructions operate.
After pelleting semi-finished product by conveyer belt enter rotating cage sizing, setting temperature be 20 DEG C, relative humidity 30% hereinafter, when
Between 3 hours.It is cleaned with 95% edible alcohol, removes the Valelinum Liquidum for being attached to capsule and pill outer surface, repeat to clean to capsule and pill surface
Completely, alcohol is drained.By disk on the capsule and pill after sizing, it is pushed into drying shed and is dried；25 DEG C of drying temperature in drying shed, relatively
Humidity 25%.Drying time 24 hours, obtain the soft capsule with aloe for intending shelf fungus containing red edge.
Embodiment 5
A kind of preparation method of soft capsule with aloe of the raw material containing the quasi- shelf fungus of red edge and tremella, includes the following steps:
1) red edge is intended into shelf fungus and is inoculated into following culture mediums: potato 250g/L, lactose 30g/L, beancake powder 5g/L, phosphoric acid
Potassium dihydrogen 5g/L, magnesium sulfate 5g/L, adjusting pH value is 7, is cultivated 2 days at 25 DEG C, and filtering intends shelf fungus fermentation liquid to get red edge；
2) tremella strain is inoculated into following culture mediums: potato 200g/L, corn flour 30g/L, glucose 25g/L, albumen
Peptone 5g/L, potassium dihydrogen phosphate 5g/L, magnesium sulfate 5g/L, VB15g/L, adjusting pH value is 6, is cultivated 3 days at 25 DEG C, is filtered to get gold
Ear bacterial strain fermentation liquor；
3) red edge described in 1000g is intended into tremella strain fermentation liquid described in shelf fungus fermentation liquid, 1500g and 1000g aloe is complete
The mixing of leaf freeze-dried powder, ferments 6 days at 25 DEG C, filters, and places, and filters, and sterilizing obtains aloe fermentation liquid；
4) 250g soybean oil is added in water-bath type warm ingredients tank (100L type, Wenzhou Kerui Machinery Co., Ltd.'s production)
Heat to addition 30g beeswax after 60 DEG C stirs 30 minutes, is down to 50 DEG C to temperature and adds aloe fermentation liquid described in 220g, side is added
Side stir about 25 minutes, aloe capsule filler is made；Aloe capsule filler is crossed into colloid mill (JM80 type, Langfang universal machine
The production of tool Manufacturing Co., Ltd), 80 meshes are crossed after stirring 2 hours, puts into stirring 30 minutes in ingredients pot and releases again after sieving, to
With.
Capsule casing material is made of gelatin, glycerol, purified water, sodium copper chlorophyllin；Gelatin: glycerol: purified water ratio be for
1:0.45:1；Sodium copper chlorophyllin dosage is 0.25g/kg.It is operated by the standard practice instructions for changing glue tank, pre- thermalization glue tank
(RJHA-200L type, Wenzhou Rongji Capsule Medicinal Chemical Equipment Co., Ltd.'s production), by process recipe requirement, is added 280g purifying
Water, 126g glycerol are heated to 70 DEG C of dissolutions；280g gelatin is added, stirs 20 minutes, is down to 70 DEG C to temperature, 0.2g leaf is added
Glue is made after five minutes, opens vacuum pump and vacuumizes to glue for green element copper sodium, stirring, and bubble is eliminated to liquid level, is stablized
30 minutes afterwards, vacuum degree 0.07mpa.According to glue is cooked after inspection appearance, viscosity are qualified, glue is put as required, crosses 100
It is banished after mesh in 60 DEG C of heat preservations in glue bucket, for use.
By aloe capsule filler obtained and capsule casing material, by processing parameter, (shape is ellipse, loading amount
500mg/, 55 DEG C of glue box temperature, temperature of charge are room temperature, control loading amount in 500mg ± 37.5mg), in pellet press (RGY6-
20 types, Changzheng Tianmin High Science & Technology Co., Ltd., Beijing production) on by pelleting standard practice instructions operate.
After pelleting semi-finished product by conveyer belt enter rotating cage sizing, setting temperature be 20 DEG C, relative humidity 30% hereinafter, when
Between 3 hours.It is cleaned with 95% edible alcohol, removes the Valelinum Liquidum for being attached to capsule and pill outer surface, repeat to clean to capsule and pill surface
Completely, alcohol is drained.By disk on the capsule and pill after sizing, it is pushed into drying shed and is dried；25 DEG C of drying temperature in drying shed, relatively
Humidity 25%.Drying time 24 hours, obtain the soft capsule with aloe for intending shelf fungus containing red edge.
Embodiment 6
A kind of preparation method of soft capsule with aloe of the raw material containing the quasi- shelf fungus of red edge and tremella, includes the following steps:
1) red edge is intended into shelf fungus and is inoculated into following culture mediums: potato 250g/L, lactose 20g/L, beancake powder 2g/L, phosphoric acid
Potassium dihydrogen 1g/L, magnesium sulfate 0.5g/L, adjusting pH value is 7, is cultivated 2 days at 26 DEG C, and filtering intends shelf fungus fermentation liquid to get red edge；
2) tremella strain is inoculated into following culture mediums: potato 200g/L, corn flour 30g/L, peptone 6g/L, glucose
20g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 3g/L, VB10.02g/L, adjust pH value be 6,25 DEG C cultivate 3 days, filtering to get
Tremella strain fermentation liquid；
3) red edge described in 500g is intended into tremella strain fermentation liquid and 1000g aloe full leaf described in shelf fungus fermentation liquid, 1500g
Freeze-dried powder mixing, ferments 6 days at 27 DEG C, filters, and places, and filters, and sterilizing obtains aloe fermentation liquid；
4) 250g soybean oil is added in water-bath type warm ingredients tank (100L type, Wenzhou Kerui Machinery Co., Ltd.'s production)
Heat to addition 30g beeswax after 60 DEG C stirs 30 minutes, is down to 50 DEG C to temperature and adds aloe fermentation liquid described in 220g, side is added
Side stir about 25 minutes, aloe capsule filler is made；Aloe capsule filler is crossed into colloid mill (JM80 type, Langfang universal machine
The production of tool Manufacturing Co., Ltd), 80 meshes are crossed after stirring 2 hours, puts into stirring 30 minutes in ingredients pot and releases again after sieving, to
With.
Capsule casing material is made of gelatin, glycerol, purified water, sodium copper chlorophyllin；Gelatin: glycerol: purified water ratio be for
1:0.45:1；Sodium copper chlorophyllin dosage is 0.25g/kg.It is operated by the standard practice instructions for changing glue tank, pre- thermalization glue tank
(RJHA-200L type, Wenzhou Rongji Capsule Medicinal Chemical Equipment Co., Ltd.'s production), by process recipe requirement, is added 280g purifying
Water, 126g glycerol are heated to 70 DEG C of dissolutions；280g gelatin is added, stirs 20 minutes, is down to 70 DEG C to temperature, 0.2g leaf is added
Glue is made after five minutes, opens vacuum pump and vacuumizes to glue for green element copper sodium, stirring, and bubble is eliminated to liquid level, is stablized
30 minutes afterwards, vacuum degree 0.07mpa.According to glue is cooked after inspection appearance, viscosity are qualified, glue is put as required, crosses 100
It is banished after mesh in 60 DEG C of heat preservations in glue bucket, for use.
By aloe capsule filler obtained and capsule casing material, by processing parameter, (shape is ellipse, loading amount
500mg/, 55 DEG C of glue box temperature, temperature of charge are room temperature, control loading amount in 500mg ± 37.5mg), in pellet press (RGY6-
20 types, Changzheng Tianmin High Science & Technology Co., Ltd., Beijing production) on by pelleting standard practice instructions operate.
After pelleting semi-finished product by conveyer belt enter rotating cage sizing, setting temperature be 20 DEG C, relative humidity 30% hereinafter, when
Between 3 hours.It is cleaned with 95% edible alcohol, removes the Valelinum Liquidum for being attached to capsule and pill outer surface, repeat to clean to capsule and pill surface
Completely, alcohol is drained.By disk on the capsule and pill after sizing, it is pushed into drying shed and is dried；25 DEG C of drying temperature in drying shed, relatively
Humidity 25%.Drying time 24 hours, obtain the soft capsule with aloe for intending shelf fungus containing red edge.
7 pharmacological testing of embodiment
Test method one:
Experimental animal: Kunming mouse, half male and half female, 18~22g of weight, by Liaoning, long-living Biotechnology Co., Ltd is mentioned
For.7 groups are randomly divided into, every group 10, wherein six groups are test group, one group is Normal group, and first group is to take embodiment 1
The soft capsule with aloe of preparation, second group is the soft capsule with aloe for taking the preparation of embodiment 2, and so on；
Test procedure: giving Kunming mouse perfusion, 1 time a day, once a grain, continuous 5 days, after last dose 30min, uses
Scissors is brief at Mouse Tail-tip, autoblood flow out when start timing, suck one with filter paper every 30s and bleed, until filter paper without
Blood be sucked out until time, be the bleeding time, same method after last dose 30min, from mouse eyeground vein Cong Quxue, is abandoned
After going First Blood, Yu Qingjie glass slide both ends are respectively bled, and drop of blood size is consistent as far as possible (diameter about 5mm or so), are started
Timing,
It is primary that with No. 6 pillows of cleaning blood is gently provoked from drop of blood edge inwards every 30s, to provoking blood since blood sampling
The time of silk position, i.e. clotting time.
Influence of the soft capsule with aloe prepared by the present invention of table 1 to the mouse bleeding time
As shown in Table 1, compared with the control group, the soft capsule with aloe of Examples 1 to 6 preparation can be obviously prolonged mouse bleeding
Time and clotting time, the extension time longest of (raw material intends shelf fungus and tremella containing red edge) that wherein prepared by embodiment 6,
(raw material contains tremella) aloe prepared by (raw material intends shelf fungus containing red edge) soft capsule with aloe and embodiment 2 prepared by embodiment 1
Soft capsule the extended time be below (raw material intends shelf fungus and tremella containing red edge) soft capsule with aloe institute of the preparation of embodiment 6
The extended time illustrates that red edge intends the secondary metabolite that shelf fungus, tremella and aloe fermentation generate, can be obviously prolonged mouse
Bleeding and clotting time, having improves microcirculation, blood coagulation resisting function.
Test method two:
Experimental animal: Wistar rat, half male and half female, 180~220g of weight, by the long-living Biotechnology Co., Ltd in Liaoning
It provides.7 groups are randomly divided into, every group 10, wherein six groups are test group, one group is Normal group, and first group is to take implementation
Soft capsule with aloe prepared by example 1, second group is the soft capsule with aloe for taking the preparation of embodiment 2, and so on；
Test procedure: being administered to Wistar rat oral gavage, 2 times a day, once a grain, continuous 14d, not in fasting on the 15th
Prohibit water, anaesthetized with 10% urethane, lie on the back and separate left vena jugularis externa and right common carotid artery, taking two sections of internal diameters is the polyethylene of 1mm
The casing of pipe composition, in one section of weighing silk thread for setting a 6cm long, with 50 μ/mL heparin-saline full of polyethylene pipe, when
50 μ/kg heparin is injected by polyethylene pipe after one end insertion vena jugularis externa of pipe, after clamping pipe, other end casing is inserted into right neck
Total artery decontrols blood flow after the completion of operation immediately, and middle clinopodium polycephalum takes out rapidly silk thread weighing after 15min, and total weight subtracts silk thread
Weight is wet weight of thrombus.
Thrombus inhibiting rate (%)=(control group thrombus weight-administration group thrombus weight)/control group thrombus weight * 100%
Influence of the soft capsule with aloe prepared by the present invention of table 2 to mouse experiment thrombosis
As shown in Table 2, compared with the control group, the soft capsule with aloe of Examples 1 to 6 preparation all has suppression to mouse antithrombotic
The thrombus inhibitory effect of effect processed, (raw material intends shelf fungus and tremella containing red edge) that wherein prepared by embodiment 6 is best, inhibiting rate
Up to 55.7%, (raw material intends shelf fungus containing red edge) soft capsule with aloe prepared by embodiment 1 is only 11.2%, prepared by embodiment 2
(raw material contain tremella) soft capsule with aloe be only 15.4%, roll over two kinds of drugs be below the preparation of embodiment 6 (raw material contains red edge
Quasi- shelf fungus and tremella) soft capsule with aloe thrombus inhibitory effect, illustrate that red edge intend shelf fungus, tremella and aloe fermentation production
Raw secondary metabolite can mitigate thrombotic weight in wet base, have the function of antithrombotic, facilitate the effect of inhibition thrombosis.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should be included within the scope of the present invention.
Claims (7)
1. a kind of soft capsule with aloe, which is characterized in that the filler including aloe fermentation liquid and soft capsule with aloe, according to mass ratio
Meter, the filler of the soft capsule with aloe includes following components:
The quality of vegetable oil is 1.1~1.2 times of aloe quality of fermentation broth, and the quality of suspending agent is aloe quality of fermentation broth
0.1~0.5 times；
The aloe fermentation liquid intends shelf fungus fermentation liquid by aloe full leaf freeze-dried powder, red edge and tremella strain fermentation liquid is fermented obtains
, according to mass ratio meter, the quality that red edge intends shelf fungus fermentation liquid is 0.5~1 times, tremella of aloe full leaf freeze-dried powder quality
The quality of strain fermentation liquid is 1~2.5 times of aloe full leaf freeze-dried powder quality.
2. a kind of soft capsule with aloe as described in claim 1, which is characterized in that the aloe fermentation liquid, vegetable oil and suspending
Agent mass ratio is 1:1.1:0.2.
3. a kind of soft capsule with aloe as claimed in claim 1 or 2, which is characterized in that the aloe full leaf freeze-dried powder, red edge are quasi-
The quality of shelf fungus fermentation liquid and tremella strain fermentation liquid is 1:0.5:1.5.
4. a kind of preparation method of soft capsule with aloe, which comprises the steps of:
1) red edge is intended into shelf fungus and is inoculated into following culture mediums: potato 250g/L, lactose 20g/L~40g/L, beancake powder 1~
10g/L, 0.5~20g/L of potassium dihydrogen phosphate, 0.5~20g/L of magnesium sulfate, adjusting pH value is 6.5~7.5, cultivates 1 at 23~28 DEG C
~2 days, filtering intended shelf fungus fermentation liquid to get red edge；
2) tremella strain is inoculated into following culture mediums: potato 200g/L, corn flour 20g/L~40g/L, glucose 10g/L~
40g/L, peptone 2g/L~10g/L, potassium dihydrogen phosphate 0.2g/L~20g/L, magnesium sulfate 0.15g/L~20g/L, VB0.01g/
L~10g/L, adjusting pH value is 5~7, is cultivated 2~3 days at 24~26 DEG C, is filtered to get tremella strain fermentation liquid；
3) the red edge is intended shelf fungus fermentation liquid, the tremella strain fermentation liquid to mix with aloe full leaf freeze-dried powder, at 25 DEG C
~30 DEG C ferment 5~7 days, filter, and place, and filter, and sterilizing obtains aloe fermentation liquid；Wherein, red edge intends shelf fungus fermentation liquid
Quality be 0.5~1.5 times of aloe full leaf freeze-dried powder quality, the quality of tremella strain fermentation liquid be aloe full leaf freeze-drying silty
1~2.5 times of amount；
4) vegetable oil and suspending agent Hybrid Heating are stirred, are mixed, grind, sieving with the aloe fermentation liquid after cooling,
To capsule casing material, pelleting washes ball for filling, dry, and sizing obtains the soft capsule with aloe；Wherein, the quality of vegetable oil is reed
1.1~1.2 times of luxuriant growth quality of fermentation broth, the quality of suspending agent are 0.1~0.5 times of aloe quality of fermentation broth.
5. the preparation method of soft capsule with aloe as claimed in claim 4, which is characterized in that the aloe fermentation liquid, vegetable oil
It is 1:1.1:0.2 with suspending agent mass ratio.
6. the preparation method of soft capsule with aloe as described in claim 4 or 5, which is characterized in that the aloe full leaf freeze-dried powder,
It is 1:0.5:1.5 that red edge, which intends shelf fungus fermentation liquid and the quality of tremella strain fermentation liquid,.
7. soft capsule with aloe as claimed in claim 1 or 2 is preparing the application in antithrombotic reagent.
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| RU2705894C1 (en) * | 2018-08-24 | 2019-11-12 | Александр Александрович Кролевец | Method for producing nanocapsules of dry aloe extract in kappa-carrageenan |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1344515A (en) * | 2000-09-26 | 2002-04-17 | 昆明医学院 | Aloe fruit vinegar beverage and its production process |
| CN1958771A (en) * | 2006-12-01 | 2007-05-09 | 黄锦秀 | Dry type fermentative wine of aloe, and production technique |
| CN101986879A (en) * | 2009-07-29 | 2011-03-23 | 株式会社宫东野草研究所 | Manufacturing method for enzyme-containing healthy food and healthy food |
| CN105167061A (en) * | 2015-09-28 | 2015-12-23 | 罗萌 | Potato health care product and preparation method and application thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1344515A (en) * | 2000-09-26 | 2002-04-17 | 昆明医学院 | Aloe fruit vinegar beverage and its production process |
| CN1958771A (en) * | 2006-12-01 | 2007-05-09 | 黄锦秀 | Dry type fermentative wine of aloe, and production technique |
| CN101986879A (en) * | 2009-07-29 | 2011-03-23 | 株式会社宫东野草研究所 | Manufacturing method for enzyme-containing healthy food and healthy food |
| CN105167061A (en) * | 2015-09-28 | 2015-12-23 | 罗萌 | Potato health care product and preparation method and application thereof |
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