CN105935143A - Manufacturing method of lucid ganoderma ferment - Google Patents
Manufacturing method of lucid ganoderma ferment Download PDFInfo
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- CN105935143A CN105935143A CN201610524776.2A CN201610524776A CN105935143A CN 105935143 A CN105935143 A CN 105935143A CN 201610524776 A CN201610524776 A CN 201610524776A CN 105935143 A CN105935143 A CN 105935143A
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Abstract
The invention discloses a manufacturing method of lucid ganoderma ferment. The manufacturing method comprises the steps of extracting lucid ganoderma, preparing a liquid culture medium, and carrying out mixed fermentation and enzymatic hydrolysis to ferment lucid ganoderma. The provided manufacturing method has the advantages that the activity and nutrients of lucid ganoderma are effectively released; the content of active components such as lucid ganoderma polysaccharide, triterpene, and the like is increased; the immunity regulating and anti-oxidation performances of lucid ganoderma ferment are improved, the taste and flavor of lucid ganoderma ferment are improved, the fermentation speed is accelerated, the fermentation time is shortened, the fermentation efficiency is improved, the lucid ganoderma ferment is user-friendly, does not contain any additive, and is healthy and environment-friendly, the using effect is good, and the popularization is easy.
Description
Technical field
The present invention relates to fermentation technical field, particularly relate to the manufacture method of a kind of Ganoderma ferment.
Background technology
Ganoderma (Ganoderma lucidum) is Basidiomycetes Polyporaceae (Polyporaceae) Ganoderma
(Ganodelma) fungus Ganoderma lucidum (Leyss. Ex Fr.) Karst. G.lucidum karst and the general name of Ganoderma G.japonicrnLloyd, have and hold up
The effect such as just consolidating, the most top grade by " herbal classic ".In recent years, chemical composition and clinical research to Ganoderma fungus are a lot of,
Research to the sexual cell spore medical value of Ganoderma is the most quite paid attention to.
Ganoderma spore brown, avette, one end is truncate, (8.5～11.2) μ m (5.2～6.9) μm, tool bilayer
Wall.Brown internal layer at each spore produces many needle-like kicks, stretches into the transparent outer layer of conidial cell wall, outer layer spore dearly
Wall is smooth.The most tough and tensile, the general chemistry of two layers of walls of Ganoderma spore parcel, physical method is difficult to be broken, therefore limits
To its chemical composition, the research of bioactive ingredients.
The virgin forest that Ganoderma is maximum in the whole nation, Changbai Mountain and the growth of Natural Reserves in Hainan optimum.But due to for many years
The excessive harvesting of the mankind, the storage of wild Ganoderma is fewer and feweri.The hepatic injury that multiple physics and chemistry and biological factor are caused by Ganoderma has
Protective effect.No matter before liver injury occurs or after generation, take Ganoderma all can the liver protecting, alleviate hepatic injury.Spirit
Sesame can promote that liver, to medicine, the metabolism of poisonous substance, has definite curative effect for toxic hepatitis.Especially chronic hepatitis, spirit
Sesame can substantially eliminate the symptom such as dizziness, weak, nauseating, uncomfortable liver area, and can effectively improve liver function, makes indices
Tend to normal.So, Ganoderma can be used for treating chronic poisoning, all kinds of chronic hepatitis, liver cirrhosis, hepatic insufficiency.Ganoderma
Can coronary artery dilator effectively, increase coronary flow, improve Myocardial Microcirculation, strengthen cardiac muscle oxygen and the supply of energy,
Therefore, myocardial ischemia is had protective effect, can be widely used for the treatment and prevention of coronary heart diseases and angina pectoris etc..It addition, clothes
With may replace disengaging of insulin suppression fatty acid after Ganoderma, the symptom such as blood glucose, glucose in urine can be improved.Water solublity in Ganoderma is many
Sugar, can alleviate the occurring degree of non-insulin-dependent diabetes mellitus.
The chemical composition of Ganoderma is complex, and because of strain used, the strain place of production, cultural method, extraction process, preparation
Method is different and different.In the sporophore of Ganoderma, mycelium and spore containing polysaccharide, ucleosides, furan derivative,
Steroid ferment class, alkaloids, protein, polypeptide, amino acids, triterpenes, sesquiterpene, organic germanium, inorganic salt etc..Spirit
Sesame polysaccharide, triterpenes are one of principle active component of Ganoderma.
Summary of the invention
The invention solves the problems that the problem that above-mentioned prior art exists, it is provided that the manufacture method of a kind of Ganoderma ferment, it is possible to increase spirit
The content of effective ingredient in sesame ferment, meets the demand of glossy ganoderma fermentation.
The present invention solve its technical problem use technical scheme: the manufacture method of this Ganoderma ferment, comprise the steps:
The first step, Ganoderma extracts, is crushed by Ganoderma, obtains Ganoderma powder, then add in Ganoderma powder after crossing 50 mesh～100 mesh
Enter drinking water and carry out decocting in water, after boiling, keep boil condition 10 minutes～30 minutes, stand 12 hours after cooling～15 little
Time, take supernatant after having stood, then in supernatant, add 95% ethanol, after stirring, stand 12 hours～15 little
Time, obtain Ganoderma ethanol solution, then Ganoderma ethanol solution is carried out heating and concentrates, until concentrated solution volume is Ganoderma ethanol
The 7%～9% of liquor capacity, it is thus achieved that Ganoderma extracting solution；
Second step, prepared by fluid medium, choose Radix Codonopsis powder, Radix Liriopes powder, Pseudobulbus Bletillae (Rhizoma Bletillae) powder, Rhizoma Atractylodis powder, sporoderm-broken Ganoderma spore
Powder, Semen sojae atricolor powder, yeast powder, glucose, vitamin B1, Mel, water are fabricated to fluid medium, wherein the use of Radix Codonopsis powder
Amount is 2 parts～4 parts, and the consumption of Radix Liriopes powder is 1 part～3 parts, and the consumption of Pseudobulbus Bletillae (Rhizoma Bletillae) powder is 1 part～2 parts, the use of Rhizoma Atractylodis powder
Amount is 2 parts～4 parts, and the consumption of Ganoderma spore powder with cellular wall broken is 6 parts～9 parts, and the consumption of Semen sojae atricolor powder is 5 parts～8 parts, yeast
The consumption of powder is 8 parts～10 parts, and the consumption of glucose is 20 parts～40 parts, and the consumption of vitamin B1 is 3 parts～4 parts,
The consumption of Mel is 2 parts～4 parts, and the consumption of water is 300 parts～400 parts；
3rd step, monomer ferments, adds in fluid medium by the Ganoderma extracting solution of acquisition, add lactic acid bacteria the most again,
Then being placed to ferment in fermentation tank, fermentation temperature is 26 DEG C～29 DEG C, and tank pressure is 0.04Mpa～0.05Mpa, aseptic
Air vent amount is 2 cubic metres per minute, treats that fermentation liquid retrogradation, pH value are down to 3.0～3.5, residual sugar amount is down to 0.2%～0.3%
After, it is thus achieved that Ganoderma fermentation liquid, while Ganoderma extracting solution ferments, set up another fermentation tank, in fermentation tank, add Fructus Ananadis comosi, sweet
Sugarcane, Hylocereus undatus, Fructus Citri Limoniae, Fructus Cucurbitae moschatae, Herba Apii graveolentis, Fructus Jujubae, Fructus actinidiae chinensis, Fructus Mali pumilae, Fructus Chaenomelis, Fructus Ananadis comosi is peeling Fructus Ananadis comosi, and Caulis Sacchari sinensis is
Peeling Caulis Sacchari sinensis, Hylocereus undatus is peeling Hylocereus undatus, and Fructus Citri Limoniae is peeling Fructus Citri Limoniae, and Fructus Cucurbitae moschatae is peeling Fructus Cucurbitae moschatae, and Fructus actinidiae chinensis is peeling macaque
Fructus Persicae, Fructus Mali pumilae is peeling Fructus Mali pumilae, and Fructus Chaenomelis is peeling Fructus Chaenomelis, then adds lactic acid bacteria in fermentation tank and ferments, fermentation temperature
Being 26 DEG C～29 DEG C, tank pressure is 0.04Mpa～0.05Mpa, and filtrated air ventilation is 2 cubic metres per minute, treats fermentation liquid
After retrogradation, pH value are down to 3.0～3.5, residual sugar amount is down to 0.2%～0.3%, it is thus achieved that fruit and vegerable fermentation liquid；
4th step, mixed fermentation, by the Ganoderma fermentation liquid obtained and the mixing of fruit and vegerable fermentation liquid, it is thus achieved that mixture, then will mixing
Thing is placed to fermentation tank, then in fermentation tank add lactic acid bacteria, carry out mixed fermentation, fermentation temperature during fermentation be 26 DEG C～
29 DEG C, tank pressure is 0.04Mpa～0.05Mpa, after fermenting 2 years, it is thus achieved that Ganoderma mixed fermentation liquid；
5th step, enzymolysis, in Ganoderma mixed fermentation liquid, addition amylase, protease, cellulase carry out enzymolysis, enzymolysis
Temperature is 50 DEG C～60 DEG C, and enzymolysis time is 5 hours～6 hours, wherein amylase, protease, cellulase and Ganoderma
The mass ratio of mixed fermentation liquid is 1:1:1:100, obtains Ganoderma ferment after enzymolysis.
Further, any one during the Ganoderma in the first step is Fructificatio Amaurodermatis Rudae, Ganoderma lucidum seu Japonicum, Ganoderma, Ganoderma lucidum (Leyss. Ex Fr.) Karst., Radix Angelicae Dahuricae, Rhizoma Polygonati.
Further, the lactic acid bacteria in the 3rd step be streptococcus thermophilus, Deshi Lactobacillus, bacillus acidophilus, lactobacillus casei,
Lactobacillus plantarum, lactobacillus rhamnosus, Lactobacillus paracasei, Pediococcus pentosaceus, Lactobacillus brevis, lactobacillus helveticus, goldbeater's skin
Any one or any three kinds of combination of the above in leukonid.
Further, after the 5th step obtains Ganoderma ferment, carry out the extraction of Ganoderma ferment, the Ganoderma ferment of acquisition is carried out
High pressure homogenize processes, extracts, concentrates, obtain solid-state Ganoderma ferment after vacuum lyophilization.
Further, high pressure homogenize uses high pressure homogenizer to carry out high pressure homogenize process when processing.
Further, supercritical carbon dioxide method for extracting is used to extract during extraction.
The manufacture method of the Ganoderma ferment of the present invention, carries out the extraction of Ganoderma, extracts Ganoderma extracting solution in the first step, and second
Prepared by the fluid medium that step carries out fermenting, add Radix Codonopsis powder, Radix Liriopes powder, Pseudobulbus Bletillae (Rhizoma Bletillae) powder, Rhizoma Atractylodis powder in liquid medium within,
Radix Codonopsis powder by Radix Codonopsis grind make, Radix Liriopes powder by Radix Liriopes grind make, Pseudobulbus Bletillae (Rhizoma Bletillae) powder by the Pseudobulbus Bletillae (Rhizoma Bletillae) grind make, Rhizoma Atractylodis powder by
Rhizoma Atractylodis grind and make, and Radix Codonopsis has effect of invigorating the spleen and replenishing QI, strengthening spleen and tonifying lung, and Radix Liriopes has YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire
Effect, the Pseudobulbus Bletillae (Rhizoma Bletillae) has effect of astringing to arrest bleeding, detumescence and promoting granulation, and Rhizoma Atractylodis have drying damp and strengthening spleen, effect of expelling wind and cold, pass through
Effect of these a few taste Chinese herbal medicine can promote glossy ganoderma fermentation, effectively releases activity and the nutritional labeling of Ganoderma, improves Ganoderma ferment
Ganoderan, the content of triterpenes effective ingredient in element, improve immunomodulating effect and the anti-oxidation efficacy of Ganoderma ferment,
Reducing Ganoderma loss, fully improve Ganoderma utilization rate, Ganoderma spore powder with cellular wall broken uses the breaking cellular wall spirit identical with fermentation glossy ganoderma kind
Ganoderma lucidum spore powder, promotes that glossy ganoderma fermentation, the 3rd step carry out monomer fermentation further, adds lactic acid bacteria fermentation, it is possible to carry during fermentation
The taste and flavor of high Ganoderma ferment, the lactic acid bacteria of interpolation can be the lactic acid bacteria of a kind, it is also possible to be multiple kind groups
The compound lactobacillus closed, it is possible to realizing different taste and flavors, the 4th step carries out mixed fermentation, obtains monomer fermentation
Ganoderma fermentation liquid carries out mixing after fermentation with fruit and vegerable fermentation liquid, and the fermented product prepared can be made after adding fruit and vegerable fermentation liquid to possess fruit
The nutritional labeling of vegetables, nutritional labeling is worth height, improves the nutritive value of fermented product, and further increases the mouth of fermented product
Sense and local flavor, the 5th step carries out enzymolysis, improves the content of effective ingredient in Ganoderma ferment further, and improves spirit further
The mouthfeel of sesame ferment, obtains last finished product Ganoderma ferment, for finished product Ganoderma ferment, it is also possible to be extracted into solid-state after enzymolysis
Ganoderma ferment, the Ganoderma ferment of acquisition is carried out high pressure homogenize process, extract, concentrate, obtain after vacuum lyophilization solid
State Ganoderma ferment, it is possible to reduce the loss of Ganoderma further, effectively extract solid-state Ganoderma ferment.
The invention have the advantages that: the manufacture method of the Ganoderma ferment of the present invention, prepared Ganoderma ferment, effectively release
The activity of Ganoderma and nutritional labeling, improve ganoderan and the content of triterpenes effective active composition in Ganoderma ferment, improves
Immunomodulating effect of Ganoderma ferment and anti-oxidation efficacy, improve the taste and flavor of Ganoderma ferment, improves fermentation speed
Degree, shortens fermentation time, improves fermentation efficiency, instant, does not contains any additive, health environment-friendly, uses
Effective, it is beneficial to promote.
Detailed description of the invention
The invention will be further described below:
Embodiment one:
The manufacture method of this Ganoderma ferment, comprises the steps:
The first step, Ganoderma extracts, is crushed by Ganoderma, obtains Ganoderma powder, then add drinking water in Ganoderma powder after crossing 50 mesh
Carry out decocting in water, keep boil condition after boiling 10 minutes, stand 12 hours after cooling, after having stood, take supernatant,
In supernatant, add 95% ethanol again, after stirring, stand 12 hours, obtain Ganoderma ethanol solution, then to Ganoderma
Ethanol solution carries out heating and concentrates, until concentrated solution volume is Ganoderma volumes of aqueous ethanol 7%, it is thus achieved that Ganoderma extracting solution；
Second step, prepared by fluid medium, choose Radix Codonopsis powder, Radix Liriopes powder, Pseudobulbus Bletillae (Rhizoma Bletillae) powder, Rhizoma Atractylodis powder, sporoderm-broken Ganoderma spore
Powder, Semen sojae atricolor powder, yeast powder, glucose, vitamin B1, Mel, water are fabricated to fluid medium, wherein the use of Radix Codonopsis powder
Amount is 2 parts, and the consumption of Radix Liriopes powder is 1 part, and the consumption of Pseudobulbus Bletillae (Rhizoma Bletillae) powder is 1 part, and the consumption of Rhizoma Atractylodis powder is 2 parts, breaking cellular wall spirit
The consumption of Ganoderma lucidum spore powder is 6 parts, and the consumption of Semen sojae atricolor powder is 5 parts, and the consumption of yeast powder is 8 parts, and the consumption of glucose is 20
Part, the consumption of vitamin B1 is 3 parts, and the consumption of Mel is 2 parts, and the consumption of water is 300 parts；
3rd step, monomer ferments, adds in fluid medium by the Ganoderma extracting solution of acquisition, add lactic acid bacteria the most again,
Then being placed to ferment in fermentation tank, fermentation temperature is 26 DEG C, and tank pressure is 0.04Mpa, and filtrated air ventilation is every
Minutes 2 cubic metres, it is down to 3.0 until fermentation liquid retrogradation, pH value, after residual sugar amount is down to 0.2%, it is thus achieved that Ganoderma fermentation liquid,
Ganoderma extracting solution fermentation while, set up another fermentation tank, in fermentation tank add Fructus Ananadis comosi, Caulis Sacchari sinensis, Hylocereus undatus, Fructus Citri Limoniae, Fructus Cucurbitae moschatae,
Herba Apii graveolentis, Fructus Jujubae, Fructus actinidiae chinensis, Fructus Mali pumilae, Fructus Chaenomelis, Fructus Ananadis comosi is peeling Fructus Ananadis comosi, and Caulis Sacchari sinensis is peeling Caulis Sacchari sinensis, and Hylocereus undatus is peeling fire
Long Guo, Fructus Citri Limoniae is peeling Fructus Citri Limoniae, and Fructus Cucurbitae moschatae is peeling Fructus Cucurbitae moschatae, and Fructus actinidiae chinensis is peeling Fructus actinidiae chinensis, and Fructus Mali pumilae is peeling Fructus Mali pumilae, Fructus Chaenomelis
For peeling Fructus Chaenomelis, then adding lactic acid bacteria in fermentation tank and ferment, fermentation temperature is 26 DEG C, and tank pressure is 0.04Mpa,
Filtrated air ventilation is 2 cubic metres per minute, is down to 3.0 until fermentation liquid retrogradation, pH value, after residual sugar amount is down to 0.2%,
Obtain fruit and vegerable fermentation liquid；
4th step, mixed fermentation, by the Ganoderma fermentation liquid obtained and the mixing of fruit and vegerable fermentation liquid, it is thus achieved that mixture, then will mixing
Thing is placed to fermentation tank, then adds lactic acid bacteria in fermentation tank, carries out mixed fermentation, and fermentation temperature during fermentation is 26 DEG C,
Tank pressure is 0.04Mpa, after fermenting 2 years, it is thus achieved that Ganoderma mixed fermentation liquid；
5th step, enzymolysis, in Ganoderma mixed fermentation liquid, addition amylase, protease, cellulase carry out enzymolysis, enzymolysis
Temperature is 50 DEG C, and enzymolysis time is 5 hours, wherein the matter of amylase, protease, cellulase and Ganoderma mixed fermentation liquid
Amount ratio is 1:1:1:100, obtains Ganoderma ferment after enzymolysis.
Further, the Ganoderma in the first step is Fructificatio Amaurodermatis Rudae.
Further, the lactic acid bacteria in the 3rd step is Deshi Lactobacillus.
Further, after the 5th step obtains Ganoderma ferment, carry out the extraction of Ganoderma ferment, the Ganoderma ferment of acquisition is carried out
High pressure homogenize processes, extracts, concentrates, obtain solid-state Ganoderma ferment after vacuum lyophilization.
Further, high pressure homogenize uses high pressure homogenizer to carry out high pressure homogenize process when processing.
Further, supercritical carbon dioxide method for extracting is used to extract during extraction.
Embodiment two:
The manufacture method of this Ganoderma ferment, comprises the steps:
The first step, Ganoderma extracts, is crushed by Ganoderma, obtains Ganoderma powder, then add drinking water in Ganoderma powder after crossing 80 mesh
Carry out decocting in water, keep boil condition after boiling 20 minutes, stand 13 hours after cooling, after having stood, take supernatant,
In supernatant, add 95% ethanol again, after stirring, stand 13 hours, obtain Ganoderma ethanol solution, then to Ganoderma
Ethanol solution carries out heating and concentrates, until concentrated solution volume is Ganoderma volumes of aqueous ethanol 8%, it is thus achieved that Ganoderma extracting solution；
Second step, prepared by fluid medium, choose Radix Codonopsis powder, Radix Liriopes powder, Pseudobulbus Bletillae (Rhizoma Bletillae) powder, Rhizoma Atractylodis powder, sporoderm-broken Ganoderma spore
Powder, Semen sojae atricolor powder, yeast powder, glucose, vitamin B1, Mel, water are fabricated to fluid medium, wherein the use of Radix Codonopsis powder
Amount is 3 parts, and the consumption of Radix Liriopes powder is 2 parts, and the consumption of Pseudobulbus Bletillae (Rhizoma Bletillae) powder is 2 parts, and the consumption of Rhizoma Atractylodis powder is 3 parts, breaking cellular wall spirit
The consumption of Ganoderma lucidum spore powder is 7 parts, and the consumption of Semen sojae atricolor powder is 7 parts, and the consumption of yeast powder is 9 parts, and the consumption of glucose is 30
Part, the consumption of vitamin B1 is 4 parts, and the consumption of Mel is 3 parts, and the consumption of water is 350 parts；
3rd step, monomer ferments, adds in fluid medium by the Ganoderma extracting solution of acquisition, add lactic acid bacteria the most again,
Then being placed to ferment in fermentation tank, fermentation temperature is 27 DEG C, and tank pressure is 0.05Mpa, and filtrated air ventilation is every
Minutes 2 cubic metres, it is down to 3.2 until fermentation liquid retrogradation, pH value, after residual sugar amount is down to 0.3%, it is thus achieved that Ganoderma fermentation liquid,
Ganoderma extracting solution fermentation while, set up another fermentation tank, in fermentation tank add Fructus Ananadis comosi, Caulis Sacchari sinensis, Hylocereus undatus, Fructus Citri Limoniae, Fructus Cucurbitae moschatae,
Herba Apii graveolentis, Fructus Jujubae, Fructus actinidiae chinensis, Fructus Mali pumilae, Fructus Chaenomelis, Fructus Ananadis comosi is peeling Fructus Ananadis comosi, and Caulis Sacchari sinensis is peeling Caulis Sacchari sinensis, and Hylocereus undatus is peeling fire
Long Guo, Fructus Citri Limoniae is peeling Fructus Citri Limoniae, and Fructus Cucurbitae moschatae is peeling Fructus Cucurbitae moschatae, and Fructus actinidiae chinensis is peeling Fructus actinidiae chinensis, and Fructus Mali pumilae is peeling Fructus Mali pumilae, Fructus Chaenomelis
For peeling Fructus Chaenomelis, then adding lactic acid bacteria in fermentation tank and ferment, fermentation temperature is 27 DEG C, and tank pressure is 0.05Mpa,
Filtrated air ventilation is 2 cubic metres per minute, is down to 3.2 until fermentation liquid retrogradation, pH value, after residual sugar amount is down to 0.3%,
Obtain fruit and vegerable fermentation liquid；
4th step, mixed fermentation, by the Ganoderma fermentation liquid obtained and the mixing of fruit and vegerable fermentation liquid, it is thus achieved that mixture, then will mixing
Thing is placed to fermentation tank, then adds lactic acid bacteria in fermentation tank, carries out mixed fermentation, and fermentation temperature during fermentation is 27 DEG C,
Tank pressure is 0.05Mpa, after fermenting 2 years, it is thus achieved that Ganoderma mixed fermentation liquid；
5th step, enzymolysis, in Ganoderma mixed fermentation liquid, addition amylase, protease, cellulase carry out enzymolysis, enzymolysis
Temperature is 55 DEG C, and enzymolysis time is 6 hours, wherein the matter of amylase, protease, cellulase and Ganoderma mixed fermentation liquid
Amount ratio is 1:1:1:100, obtains Ganoderma ferment after enzymolysis.
Further, the Ganoderma in the first step is Ganoderma.
Further, the lactic acid bacteria Leuconostoc mesenteroides in the 3rd step.
Further, after the 5th step obtains Ganoderma ferment, carry out the extraction of Ganoderma ferment, the Ganoderma ferment of acquisition is carried out
High pressure homogenize processes, extracts, concentrates, obtain solid-state Ganoderma ferment after vacuum lyophilization.
Further, high pressure homogenize uses high pressure homogenizer to carry out high pressure homogenize process when processing.
Further, supercritical carbon dioxide method for extracting is used to extract during extraction.
Embodiment three:
The manufacture method of this Ganoderma ferment, comprises the steps:
The first step, Ganoderma extracts, is crushed by Ganoderma, obtains Ganoderma powder, then add in Ganoderma powder and drink after crossing 100 mesh
Water carries out decocting in water, keeps boil condition 30 minutes after boiling, and stands 15 hours after cooling, takes supernatant after having stood,
In supernatant, add 95% ethanol again, after stirring, stand 15 hours, obtain Ganoderma ethanol solution, then to Ganoderma
Ethanol solution carries out heating and concentrates, until concentrated solution volume is Ganoderma volumes of aqueous ethanol 9%, it is thus achieved that Ganoderma extracting solution；
Second step, prepared by fluid medium, choose Radix Codonopsis powder, Radix Liriopes powder, Pseudobulbus Bletillae (Rhizoma Bletillae) powder, Rhizoma Atractylodis powder, sporoderm-broken Ganoderma spore
Powder, Semen sojae atricolor powder, yeast powder, glucose, vitamin B1, Mel, water are fabricated to fluid medium, wherein the use of Radix Codonopsis powder
Amount is 4 parts, and the consumption of Radix Liriopes powder is 3 parts, and the consumption of Pseudobulbus Bletillae (Rhizoma Bletillae) powder is 2 parts, and the consumption of Rhizoma Atractylodis powder is 4 parts, breaking cellular wall spirit
The consumption of Ganoderma lucidum spore powder is 9 parts, and the consumption of Semen sojae atricolor powder is 8 parts, and the consumption of yeast powder is 10 parts, and the consumption of glucose is 40
Part, the consumption of vitamin B1 is 4 parts, and the consumption of Mel is 4 parts, and the consumption of water is 400 parts；
3rd step, monomer ferments, adds in fluid medium by the Ganoderma extracting solution of acquisition, add lactic acid bacteria the most again,
Then being placed to ferment in fermentation tank, fermentation temperature is 29 DEG C, and tank pressure is 0.05Mpa, and filtrated air ventilation is every
Minutes 2 cubic metres, it is down to 3.5 until fermentation liquid retrogradation, pH value, after residual sugar amount is down to 0.3%, it is thus achieved that Ganoderma fermentation liquid,
Ganoderma extracting solution fermentation while, set up another fermentation tank, in fermentation tank add Fructus Ananadis comosi, Caulis Sacchari sinensis, Hylocereus undatus, Fructus Citri Limoniae, Fructus Cucurbitae moschatae,
Herba Apii graveolentis, Fructus Jujubae, Fructus actinidiae chinensis, Fructus Mali pumilae, Fructus Chaenomelis, Fructus Ananadis comosi is peeling Fructus Ananadis comosi, and Caulis Sacchari sinensis is peeling Caulis Sacchari sinensis, and Hylocereus undatus is peeling fire
Long Guo, Fructus Citri Limoniae is peeling Fructus Citri Limoniae, and Fructus Cucurbitae moschatae is peeling Fructus Cucurbitae moschatae, and Fructus actinidiae chinensis is peeling Fructus actinidiae chinensis, and Fructus Mali pumilae is peeling Fructus Mali pumilae, Fructus Chaenomelis
For peeling Fructus Chaenomelis, then adding lactic acid bacteria in fermentation tank and ferment, fermentation temperature is 29 DEG C, and tank pressure is 0.05Mpa,
Filtrated air ventilation is 2 cubic metres per minute, is down to 3.5 until fermentation liquid retrogradation, pH value, after residual sugar amount is down to 0.3%,
Obtain fruit and vegerable fermentation liquid；
4th step, mixed fermentation, by the Ganoderma fermentation liquid obtained and the mixing of fruit and vegerable fermentation liquid, it is thus achieved that mixture, then will mixing
Thing is placed to fermentation tank, then adds lactic acid bacteria in fermentation tank, carries out mixed fermentation, and fermentation temperature during fermentation is 29 DEG C,
Tank pressure is 0.05Mpa, after fermenting 2 years, it is thus achieved that Ganoderma mixed fermentation liquid；
5th step, enzymolysis, in Ganoderma mixed fermentation liquid, addition amylase, protease, cellulase carry out enzymolysis, enzymolysis
Temperature is 60 DEG C, and enzymolysis time is 6 hours, wherein the matter of amylase, protease, cellulase and Ganoderma mixed fermentation liquid
Amount ratio is 1:1:1:100, obtains Ganoderma ferment after enzymolysis.
Further, the Ganoderma in the first step is Ganoderma lucidum (Leyss. Ex Fr.) Karst..
Further, the combination that lactic acid bacteria is bacillus acidophilus, lactobacillus casei, Lactobacillus plantarum in the 3rd step.
Further, after the 5th step obtains Ganoderma ferment, carry out the extraction of Ganoderma ferment, the Ganoderma ferment of acquisition is carried out
High pressure homogenize processes, extracts, concentrates, obtain solid-state Ganoderma ferment after vacuum lyophilization.
Further, high pressure homogenize uses high pressure homogenizer to carry out high pressure homogenize process when processing.
Further, supercritical carbon dioxide method for extracting is used to extract during extraction.
In the embodiment of the present invention one, embodiment two, embodiment three, 1 part is 1 gram.
Choose the embodiment of the present invention one, embodiment two, the Ganoderma ferment of embodiment three preparation and Common ganoderma lucidum ferment to contrast,
Comparing result is as follows:
As can be known from the above table, the Ganoderma ferment of the embodiment of the present invention one, embodiment two, embodiment three preparation, effectively become in Ganoderma
Dividing content to be above Common ganoderma lucidum ferment, mouthfeel is superior to Common ganoderma lucidum ferment.
The feature of the embodiment of the present invention is: the Ganoderma ferment prepared, and effectively releases activity and the nutritional labeling of Ganoderma, improves
Ganoderan and the content of triterpenes effective active composition in Ganoderma ferment, improve Ganoderma ferment immunomodulating effect and
Anti-oxidation efficacy, improves the taste and flavor of Ganoderma ferment, improves fermenting speed, shorten fermentation time, improve
Fermentation efficiency, instant, do not contain any additive, health environment-friendly, using effect is good, is beneficial to promote.
Although the present invention is by being described with reference to preferred embodiment, but, those skilled in the art should
Solve, in the range of claims, the various change in form and details can be made.
Claims (6)
1. the manufacture method of a Ganoderma ferment, it is characterised in that comprise the steps:
(1) Ganoderma extraction, crushes Ganoderma, obtains Ganoderma powder, then add drink in Ganoderma powder after crossing 50 mesh～100 mesh
Carry out decocting in water with water, keep boil condition after boiling 10 minutes～30 minutes, stand 12 hours～15 hours after cooling,
Standing takes supernatant after completing, then adds 95% ethanol in supernatant, after stirring, stands 12 hours～15 hours,
Obtain Ganoderma ethanol solution, then Ganoderma ethanol solution is carried out heating and concentrates, until concentrated solution volume is Ganoderma ethanol solution
The 7%～9% of volume, it is thus achieved that Ganoderma extracting solution；
(2) prepared by fluid medium, choose Radix Codonopsis powder, Radix Liriopes powder, Pseudobulbus Bletillae (Rhizoma Bletillae) powder, Rhizoma Atractylodis powder, Ganoderma spore powder with cellular wall broken,
Semen sojae atricolor powder, yeast powder, glucose, vitamin B1, Mel, water are fabricated to fluid medium, and wherein the consumption of Radix Codonopsis powder is
2 parts～4 parts, the consumption of Radix Liriopes powder is 1 part～3 parts, and the consumption of Pseudobulbus Bletillae (Rhizoma Bletillae) powder is 1 part～2 parts, and the consumption of Rhizoma Atractylodis powder is 2
Part～4 parts, the consumption of Ganoderma spore powder with cellular wall broken is 6 parts～9 parts, and the consumption of Semen sojae atricolor powder is 5 parts～8 parts, the use of yeast powder
Amount is 8 parts～10 parts, and the consumption of glucose is 20 parts～40 parts, and the consumption of vitamin B1 is 3 parts～4 parts, Mel
Consumption is 2 parts～4 parts, and the consumption of water is 300 parts～400 parts；
(3) monomer fermentation, adds in fluid medium by the Ganoderma extracting solution of acquisition, adds lactic acid bacteria the most again, then
Being placed in fermentation tank ferment, fermentation temperature is 26 DEG C～29 DEG C, and tank pressure is 0.04Mpa～0.05Mpa, filtrated air
Ventilation is 2 cubic metres per minute, treats that fermentation liquid retrogradation, pH value are down to 3.0～3.5, residual sugar amount is down to 0.2%～0.3%
After, it is thus achieved that Ganoderma fermentation liquid, while Ganoderma extracting solution ferments, set up another fermentation tank, in fermentation tank, add Fructus Ananadis comosi, sweet
Sugarcane, Hylocereus undatus, Fructus Citri Limoniae, Fructus Cucurbitae moschatae, Herba Apii graveolentis, Fructus Jujubae, Fructus actinidiae chinensis, Fructus Mali pumilae, Fructus Chaenomelis, Fructus Ananadis comosi is peeling Fructus Ananadis comosi, and Caulis Sacchari sinensis is
Peeling Caulis Sacchari sinensis, Hylocereus undatus is peeling Hylocereus undatus, and Fructus Citri Limoniae is peeling Fructus Citri Limoniae, and Fructus Cucurbitae moschatae is peeling Fructus Cucurbitae moschatae, and Fructus actinidiae chinensis is peeling macaque
Fructus Persicae, Fructus Mali pumilae is peeling Fructus Mali pumilae, and Fructus Chaenomelis is peeling Fructus Chaenomelis, then adds lactic acid bacteria in fermentation tank and ferments, fermentation temperature
Being 26 DEG C～29 DEG C, tank pressure is 0.04Mpa～0.05Mpa, and filtrated air ventilation is 2 cubic metres per minute, treats fermentation liquid
After retrogradation, pH value are down to 3.0～3.5, residual sugar amount is down to 0.2%～0.3%, it is thus achieved that fruit and vegerable fermentation liquid；
(4) mixed fermentation, by the Ganoderma fermentation liquid obtained and the mixing of fruit and vegerable fermentation liquid, it is thus achieved that mixture, then puts mixture
Entering to fermentation tank, then add lactic acid bacteria in fermentation tank, carry out mixed fermentation, fermentation temperature during fermentation is 26 DEG C～29
DEG C, tank pressure is 0.04Mpa～0.05Mpa, after fermenting 2 years, it is thus achieved that Ganoderma mixed fermentation liquid；
(5) enzymolysis, in Ganoderma mixed fermentation liquid, addition amylase, protease, cellulase carry out enzymolysis, hydrolysis temperature
Being 50 DEG C～60 DEG C, enzymolysis time is 5 hours～6 hours, and wherein amylase, protease, cellulase mix with Ganoderma
The mass ratio of fermentation liquid is 1:1:1:100, obtains Ganoderma ferment after enzymolysis.
The manufacture method of Ganoderma ferment the most according to claim 1, it is characterised in that: the spirit in described step (1)
Sesame is any one in Fructificatio Amaurodermatis Rudae, Ganoderma lucidum seu Japonicum, Ganoderma, Ganoderma lucidum (Leyss. Ex Fr.) Karst., Radix Angelicae Dahuricae, Rhizoma Polygonati.
The manufacture method of Ganoderma ferment the most according to claim 1, it is characterised in that: the breast in described step (3)
Acid bacterium be streptococcus thermophilus, Deshi Lactobacillus, bacillus acidophilus, lactobacillus casei, Lactobacillus plantarum, lactobacillus rhamnosus,
In Lactobacillus paracasei, Pediococcus pentosaceus, Lactobacillus brevis, lactobacillus helveticus, Leuconostoc mesenteroides any one or arbitrarily
Three kinds of combination of the above.
The manufacture method of Ganoderma ferment the most according to claim 1, it is characterised in that: described step (5) obtains
After Ganoderma ferment, carry out the extraction of Ganoderma ferment, the Ganoderma ferment of acquisition is carried out high pressure homogenize process, extract, concentrate,
Solid-state Ganoderma ferment is obtained after vacuum lyophilization.
The manufacture method of Ganoderma ferment the most according to claim 4, it is characterised in that: described step (5) mesohigh
High pressure homogenizer is used to carry out high pressure homogenize process when homogenizing processes.
The manufacture method of Ganoderma ferment the most according to claim 4, it is characterised in that: extraction in described step (5)
Shi Caiyong supercritical carbon dioxide method for extracting extracts.
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