KR101822425B1 - Method of manufacturing bean yogurt using fermented black garlic - Google Patents
Method of manufacturing bean yogurt using fermented black garlic Download PDFInfo
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- KR101822425B1 KR101822425B1 KR1020160111172A KR20160111172A KR101822425B1 KR 101822425 B1 KR101822425 B1 KR 101822425B1 KR 1020160111172 A KR1020160111172 A KR 1020160111172A KR 20160111172 A KR20160111172 A KR 20160111172A KR 101822425 B1 KR101822425 B1 KR 101822425B1
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- South Korea
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- black garlic
- soybean
- lactic acid
- fermented black
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1315—Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/133—Fruit or vegetables
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/212—Garlic
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/24—Heat, thermal treatment
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/36—Modification in general
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/38—Multiple-step
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
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- A23Y2220/00—
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biophysics (AREA)
- Beans For Foods Or Fodder (AREA)
- Dairy Products (AREA)
Abstract
Description
본 발명은 발효 흑마늘을 이용한 콩 요거트 제조방법에 관한 것으로, 더욱 상세하게는 생마늘을 유산균 생산물질을 이용하여 흑마늘로 발효시켜 알리신의 함유량을 높이고 체내 흡수율을 향상시킬 수 있는 콩 요거트 제조방법에 관한 것이다.The present invention relates to a process for producing soybean yoghurt using fermented black garlic, and more particularly, to a method for producing soybean yoghurt by fermenting raw garlic with black garlic using a lactic acid bacteria producing material to increase the content of allysine and improve the absorption rate in the body .
특히, 본 발명은 콩의 전성분을 유산균 접종하여 만들어진 요거트에 발효된 흑마늘을 첨가한 콩 요거트 제조방법에 관한 것이다.In particular, the present invention relates to a method for producing soybean yoghurt in which black garlic fermented in yogurt prepared by lactic acid bacteria inoculation of all components of soybean is added.
대한민국 등록특허 제1174796호(등록일자: 2012.8.10., 발명의 명칭: 흑마늘 추출물을 이용한 요구르트 제조방법)는 (a)흑마늘 추출물을 준비하는 단계; (b)상기 흑마늘 추출물을 물, 탈지분유 및 글루코오스와 혼합하는 단계; (c)상기 혼합물을 살균하는 단계; (d)상기 살균 처리된 혼합물에 유산균주를 접종하여 배양하는 단계; (e)상기 배양물을 균질화하는 단계;를 포함하여 이루어지되, 상기 (a)단계의 흑마늘 추출물은 흑마늘을 열수 추출하여 55~65brix로 농축한 흑마늘 농축액이며, 상기 (b)단계는, 물 100중량부에 대하여 탈지분유 4~18중량부, 흑마늘 농축액 0.5~2.0중량부, 글루코오스 0.8~1.5중량부로 혼합하면서 이루어지며, 상기 (d)단계는, 유산균주를 접종하여 40±3℃에서 18~28시간 발효하면서 이루어지는 것을 특징으로 하는 흑마늘 추출물을 이용한 요구르트 제조방법이 개시되어 있다.Korean Patent No. 1174796 (registered on August 8, 2012, entitled "Yogurt Production Method Using Black Garlic Extract") comprises: (a) preparing a black garlic extract; (b) mixing the black garlic extract with water, skimmed milk and glucose; (c) sterilizing the mixture; (d) inoculating and culturing the lactic acid bacteria in the sterilized mixture; (a), the black garlic extract is a black garlic concentrate concentrated by hot water extraction of black garlic to a concentration of 55 to 65 brix, and the step (b) Wherein the lactic acid bacteria are inoculated in an amount of 4 to 18 parts by weight of skimmed milk powder, 0.5 to 2.0 parts by weight of a black garlic concentrate and 0.8 to 1.5 parts by weight of glucose, Wherein the fermented milk is fermented for 28 hours.
이러한 종래 기술은, 흑마늘의 유효성분을 요구르트와 함께 제공할 수 있는 반면, 흑마늘의 유효 성분인 알리신의 함유율이 높은 고품질의 콩 요구르트를 제조할 수 없는 근원적인 문제점이 있었다.This prior art has a fundamental problem in that it can not produce high quality soy yogurt having a high content of allysine, which is an effective component of black garlic, while it can provide an effective component of black garlic together with yogurt.
상기와 같은 문제점을 해결하고자 창출된 본 발명의 목적은, 콩의 전성분을 유산균 접종하여 만들어진 요거트에 발효된 흑마늘을 첨가한 발효 흑마늘을 이용한 콩 요거트 제조방법을 제공하는 데 있다.It is an object of the present invention, which is created to solve the above-mentioned problems, to provide a method for producing soybean yoghurt using fermented black garlic added with black garlic fermented in yogurt prepared by lactic acid bacteria inoculation of all components of soybean.
이러한 본 발명의 목적은, (a) 생마늘을 유산균 생성물질에 침지하여 발효 흑마늘을 만든 후 분쇄하여 발효 흑마늘 분말을 제조하는 단계와; (b) 두유액 100 중량부에 대하여 유산균 3 ~ 10 중량부를 접종시켜 6 ~ 10시간 정치시켜 발효하여 콩 요거트를 제조하는 단계와; (c) 상기 콩 요거트의 pH가 4.0 ~ 4.5일 때 상기 (b)단계의 콩 요거트 100 부피%에 대하여 상기 발효 흑마늘 분말 5 ~ 20 부피% 및 송진가루 혼합 발효액 0.1 ~ 0.3 부피%를 첨가한 후 교반한 다음, 2 ~ 5℃로 급속냉각하는 단계로 이루어지되, 상기 송진가루 혼합 발효액은, (차) 송진가루 100 중량부에 대하여, 말린 솔잎 40 ~ 50 중량부, 구연산 0.1 ~ 0.3 중량부 및 설탕 30 ~ 35 중량부를 충전한 후 5 ~ 10℃에서 12 ~16개월 발효시킨 다음 여과하는 단계와; (카) 정제수 100 ~ 500 중량부 및 천일염 4 ~ 5 중량부를 더 첨가한 후 80 ~ 100℃ 온도에서 5 ~ 10분간 끓인 다음 냉각한 다음 여과하여 제조되는 단계로 제조되는 것을 특징으로 하는 발효 흑마늘을 이용한 콩 요거트 제조방법에 의해 달성될 수 있다.(A) immersing raw garlic in a lactic acid bacteria-producing material to produce fermented black garlic, and then pulverizing the fermented black garlic to produce fermented black garlic powder; (b) inoculating 3 to 10 parts by weight of lactic acid bacterium to 100 parts by weight of soybean milk, fermenting the soybean milk at a temperature of 6 to 10 hours to produce soybean yogurt; (c) 5 to 20% by volume of the fermented black garlic powder and 0.1 to 0.3% by volume of the mixed powder of the rosin powder are added to 100% by volume of the soybean yoghurt in the step (b) when the pH of the soybean yogurt is 4.0 to 4.5 Stirring the mixture and rapidly cooling the mixture to 2 to 5 캜, wherein the mixed powder of the rosin powder comprises 40 to 50 parts by weight of dried pine leaves, 0.1 to 0.3 parts by weight of citric acid, 30 to 35 parts by weight of sugar, followed by fermentation at 5 to 10 DEG C for 12 to 16 months, followed by filtration; (K) 100 to 500 parts by weight of purified water and 4 to 5 parts by weight of a salt of the sun, followed by boiling at a temperature of 80 to 100 ° C for 5 to 10 minutes, followed by cooling, followed by filtration. Can be achieved by a method of producing soybean yogurt.
바람직하게는, 본 발명의 상기 (a)단계는, (가) 생마늘을 5 ~ 30℃의 정제수를 이용하여 적어도 1회 이상 세척하여 표면의 이물질을 제거한 후 표피를 제거하는 단계와; (나) 아미노산 및 유산균이 함유된 유산균 생성물질 100 부피%에 대하여 상기 세척된 생마늘을 30 ~ 50 부피% 비율로 침지하여 40 ~ 45℃에서 3 ~ 5일 동안 발효하여 발효 흑마늘을 제조하는 단계와; (다) 상기 발효 흑마늘을 분쇄하여 분말로 만드는 단계로 이루어지되, 상기 (나)단계의 유산균은 락토바실루스 속에는 아시도필루스, 카세이, 가쎄리, 불가리쿠스, 헬베티쿠스, 퍼멘텀, 파라카세이, 플란타럼, 루테리, 람노서스, 살리바리우스가 포함되고, 락토코커스 속에는 락티스가 포함되고, 엔테로코커스 속에는 페시움, 페칼리스가 포함되고, 스트렙토코커스 속에는 써모필루스가 포함되고, 비피도박테리움 속에는 비피덤, 브레베, 롱검, 락티스 중 선택된 1종 이상인 것을 포함하고, 상기 아미노산은 발린, 루신, 아이소루이신, 메티오닌, 트레오닌, 라이신, 페닐알라닌, 트립토판, 히스티딘인 것을 특징으로 한다.Preferably, the step (a) of the present invention comprises the steps of: (a) washing fresh garlic at least once with purified water at 5 to 30 ° C to remove foreign substances on the surface and then removing the epidermis; (B) fermenting the fermented black garlic by immersing the washed raw garlic in a proportion of 30 to 50% by volume with respect to 100% by volume of the lactic acid bacteria-producing material containing amino acid and lactic acid bacterium at 40 to 45 ° C for 3 to 5 days, ; (C) pulverizing the fermented black garlic to obtain a powder. The lactic acid bacteria in the step (B) are selected from the group consisting of Ashidopilus, Kasei, Gasseri, Bulgarian, Helveticus, , Plutarum, Luteri, Lymphocus, Salivarius, Lactococcus is contained Lactis, Enterococcus is Pethium, Pecalis, Streptococcus is Thermophilus, Bifidobacterium, Wherein the amino acid is at least one selected from the group consisting of bifidob, brep, long gom, and lactis, wherein the amino acid is valine, leucine, isoleucine, methionine, threonine, lysine, phenylalanine, tryptophan and histidine.
바람직하게는, 본 발명의 상기 (b)단계의 두유액은, (라) 생콩을 5 ~ 30℃의 정제수를 이용하여 적어도 1회 이상 세척하여 표면의 이물질을 제거하되, 수용성 영양성분의 유출을 방지하기 위하여 30분 이내로 세척한 후 10 ~ 30℃의 저온 냉풍으로 건조하거나 자연 건조하는 단계와; (마) 건조된 생콩을 분쇄기를 이용하여 80 ~ 170㎛의 입경(particle size)을 갖도록 분쇄하여 생콩가루를 제조하는 단계와; (바) 물 100 중량부에 대하여 상기 생콩가루 30 ~ 50 중량부를 혼합한 두유액을 90 ~ 120℃에서 40 ~ 90분간 끓여 살균하는 단계에 의해 제조되고, 상기 (b)단계의 유산균은 락토바실루스 속에는 아시도필루스, 카세이, 가쎄리, 불가리쿠스, 헬베티쿠스, 퍼멘텀, 파라카세이, 플란타럼, 루테리, 람노서스, 살리바리우스가 포함되고, 락토코커스 속에는 락티스가 포함되고, 엔테로코커스 속에는 페시움, 페칼리스가 포함되고, 스트렙토코커스 속에는 써모필루스가 포함되고, 비피도박테리움 속에는 비피덤, 브레베, 롱검, 락티스 중 선택된 1종 이상인 것을 특징으로 한다.Preferably, the soybean milk of the step (b) of the present invention is prepared by: (d) washing soy beans with purified water at 5 to 30 ° C. at least once to remove foreign substances from the surface, And drying or naturally drying with a cold cold air at 10 to 30 DEG C; (E) pulverizing dried soybeans to have a particle size of 80 to 170 mu m using a pulverizer to prepare raw soy flour; (F) 30 to 50 parts by weight of the raw soybean flour is blended with 100 parts by weight of water to boil the soy milk at 90 to 120 ° C for 40 to 90 minutes, and the lactic acid bacterium of step (b) It includes Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, Lactobacillus, It is characterized in that it contains one or more selected from the group consisting of bifidoblem, breve, long gom, and lactis in the genus Bifidobacterium.
보다 바람직하게는, 본 발명의 상기 (나)단계에 상기 아미노산 및 유산균이 함유된 유산균 생성물질 100 부피%에 대하여 로스팅된 와송 분말을 1 ~ 2 부피%를 더 첨가하고, 상기 로스팅 와송 분말은, (사) 꽃잎, 씨방, 잎 및 줄기를 모두 포함하여 건조된 와송을 60 ~ 100℃에서 5 ~ 10분간 증숙한 다음 35 ~ 40℃로 냉각하는 단계와; (아) 상기 냉각된 와송을 1 ~ 2㎜의 입경을 갖도록 분쇄하여 와송 분말을 만드는 단계와; (자) 180 ~ 230℃로 가열된 로스터에서 상기 와송 분말을 로스팅한 다음, 30 ~ 35℃로 급속냉각하여 제조되는 단계로 제조되는 것을 특징으로 한다.More preferably, in step (b) of the present invention, 1 to 2 volume% of rosin powder is further added to 100 volume% of the lactic acid bacterium-producing material containing the amino acid and the lactic acid bacterium, (G) mixing the dried petioles, ovaries, leaves and stems together at 60 to 100 ° C for 5 to 10 minutes and cooling to 35 to 40 ° C; (A) pulverizing the cooled pulp to have a particle diameter of 1 to 2 mm to produce a pulverized powder; And roasting the raw powder in a roaster heated at 180 to 230 ° C, followed by rapid cooling to 30 to 35 ° C.
삭제delete
이상과 같은 본 발명은 다음과 같은 효과가 있다.The present invention has the following effects.
첫째, 본 발명은 콩에 함유되지 않은 필수 아미노산 9가지를 간편하게 섭취할 수 있다.First, the present invention can easily ingest nine essential amino acids not contained in soy.
둘째, 본 발명은 분해가 어려운 콩의 단백질을 아미노산으로 저분자화시켜 체내 단백질 흡수를 높일 수 있다.Second, the present invention can increase protein absorption by lowering the protein of soybean which is difficult to decompose with amino acid.
셋째, 본 발명은 마늘의 유효 성분인 알리신을 유산균 생성물질을 이용한 발효를 통해 함유율 및 체내 흡수율을 높일 수 있다.Thirdly, the content of allysine, which is an active ingredient of garlic, can be increased through fermentation using lactic acid bacteria-producing material and the absorption rate in the body.
넷째, 본 발명은 유산균 생성물질을 통해 생마늘을 발효시켜 발효 흑마늘을 제조하고, 콩의 전성분을 유산균 접종하여 만들어진 요커트에 발효 흑마늘을 첨가함으로써 마늘의 알리신 성분, 필수 아미노산 및 저분자화된 콩 단백질을 동시에 섭취할 수 있는 효과가 있다.Fourthly, the present invention relates to a method for producing fermented black garlic by fermenting raw garlic through a lactic acid producing material and adding fermented black garlic to a yorkette produced by lactic acid germ inoculation of all the components of the soybean to obtain an allysine component, an essential amino acid and a low- Can be consumed at the same time.
이하, 본 발명의 실시예를 참조하여 설명하기로 한다.Hereinafter, embodiments of the present invention will be described.
1. 발효 흑마늘 제조 1. Fermented black garlic manufacturing
가. 흑마늘 세척end. Black garlic wash
생마늘을 5 ~ 30℃의 정제수를 이용하여 적어도 1회 이상 세척하여 표면의 이물질을 제거한다.Fresh raw garlic is washed at least once with purified water at 5 ~ 30 ℃ to remove foreign substances on the surface.
본 실시예에서 사용되는 생마늘을 표피(껍질)을 제거한 것을 사용한다.The raw garlic used in this embodiment is one obtained by removing the skin.
나. 발효I. Fermentation
아미노산 및 유산균이 함유된 유산균 생성물질 100 부피%에 대하여 세척된 생마늘 30 ~ 50 부피%를 침지하여 40 ~ 45℃에서 3 ~ 5일 동안 발효하여 발효 흑마늘을 제조한다.30 to 50% by volume of washed fresh germanium is immersed in 100% by volume of lactic acid bacteria-producing material containing amino acids and lactic acid bacteria, and fermented at 40 to 45 ° C for 3 to 5 days to produce fermented black garlic.
본 실시예에서 정의하고 있는 유산균 생성물질(Biogenics)은 1종 이상의 유산균을 우유나 콩과 같은 배지를 이용하여 발효과정을 통해 생산되는 유용한 대사물질을 의미하며 4 ~ 5pH의 산도를 갖는다.The biogenics defined in this embodiment means a useful metabolite produced by fermentation using one or more kinds of lactic acid bacteria in a medium such as milk or soybean and has an acidity of 4 to 5 pH.
여기서, 본 실시예에 사용되는 유산균은 대한민국 식품의약품안전처가 기능성 원재료로 인정하고 있는 락토바실루스, 락토코커스, 엔테로코커스, 스트렙토코커스 및 비피도박테리움 등 5가지 속에 포함되는 19종으로, 락토바실루스 속에는 아시도필루스, 카세이, 가쎄리, 불가리쿠스, 헬베티쿠스, 퍼멘텀, 파라카세이, 플란타럼, 루테리, 람노서스, 살리바리우스 등의 11종이 포함되고, 락토코커스 속에는 락티스 1종이 포함되고, 엔테로코커스 속에는 페시움, 페칼리스 등의 2종이 포함되고, 스트렙토코커스 속에는 써모필루스 1종이 포함되고, 비피도박테리움 속에는 비피덤, 브레베, 롱검, 락티스 등의 4종이 포함된다.Herein, the lactic acid bacteria used in this embodiment are 19 kinds contained in five kinds of lactic acid bacteria, Lactobacillus, Lactococcus, Enterococcus, Streptococcus and Bifidobacterium, which are recognized as functional raw materials by Korea Food and Drug Administration, 11 species such as Acidophilus, Kasei, Gasseri, Bulgarian, Helveticus, Permentum, Paracasei, Pterantrum, Luterian, Laminocus and Salivarius. Lactococcus contains 1 Lactis In the Enterococcus, there are two species such as Pesium and Pecalis. Streptococcus species includes one species of Thermophilus. Bifidobacterium contains four species such as Bifidum, Breve, Longgem, and Lactis.
또한, 유산균 생성물질은 우유 또는 두유 1,000ml에서 동결건조된 유산균 10 ~ 200g 첨가하여 배양한 것이며, 이에 대한 배양방법은 공지의 기술이기 때문에 이에 대한 상세한 설명은 생략하기로 한다.The lactic acid bacteria-producing material is prepared by adding 10-200 g of freeze-dried lactic acid bacterium in 1,000 ml of milk or soybean milk, and the culture method is well known in the art, so a detailed description thereof will be omitted.
그리고 유산균 생산물질은 장 내의 유해균 억제기능이 있다.And the lactic acid producing material has the ability to inhibit harmful bacteria in the intestines.
본 실시예에 사용되는 유산균 생산물질은 발린(valine), 루신(leucine), 아이소루이신(isoleucine), 메티오닌(methionine), 트레오닌(threonine), 라이신(lysine), 페닐알라닌(phenylalanine), 트립토판(tryptophan), 히스티딘(Histidine) 등의 필수 아미노산을 포함하고 있으나, 별도로 1일 복용 권장량을 추가할 수 있음을 미리 밝혀둔다.The lactic acid bacteria producing material used in this embodiment is preferably selected from the group consisting of valine, leucine, isoleucine, methionine, threonine, lysine, phenylalanine, tryptophan ), And histidine (Histidine), but it is possible to add a recommended daily dose separately.
또한, 마늘이나 흑마늘의 유효 성분이 사포닌은 특유의 씁쓸한 풍미가 있는데, 이를 제거하거나 중화하기 위하여 아미노산 및 유산균이 함유된 유산균 생성물질 100 부피%에 대하여 로스팅된 와송 분말을 1 ~ 2 부피%를 더 첨가하여 발효시킬 수 있다. 여기서, 와송은 돌나물과에 속하는 여러해살이 풀로서 성질이 차서 열을 내리고 수렴하는 성질이 있으므로 양혈하여 지혈하는데 효과가 있는 약초이다.In addition, saponin, which is an effective ingredient of garlic or black garlic, has a bitter flavor. In order to remove or neutralize saponin, 1 to 2% by volume of roasted powdery powder is added to 100% by volume of the lactic acid bacteria producing material containing amino acids and lactic acid bacteria Can be added and fermented. Here, Wassong is a perennial herb that belongs to the family Staphylococcus aureus, and has a property of lowering heat and converging.
로스팅된 와송 분말은 다음과 같은 제조공정에 의해 제조된다.The roasted feed powder is prepared by the following production process.
① 꽃잎, 씨방, 잎 및 줄기를 모두 포함하여 건조된 와송을 60 ~ 100℃에서 5 ~ 10분간 증숙한 다음 35 ~ 40℃로 냉각한다.① Include petals, ovaries, leaves and stems, and dry them at 60 ~ 100 ℃ for 5 ~ 10 minutes and cool to 35 ~ 40 ℃.
② 냉각된 와송을 1 ~ 2㎜의 입경을 갖도록 분쇄하여 와송 분말을 만든다.② Crush the cooled feedstock to have a particle size of 1 ~ 2 mm to make a wastepaper powder.
③ 180 ~ 230℃로 가열된 로스터에서 와송 분말을 로스팅한 다음, 30 ~ 35℃로 급속냉각하여 포장한다.③ Raw powder is roasted in a roaster heated at 180 ~ 230 ℃, then cooled rapidly at 30 ~ 35 ℃ and wrapped.
다. 분쇄All. smash
발효 흑마늘을 분쇄하여 분말로 만들어 진공 포장한다.Fermented black garlic is crushed to powder and vacuum packaged.
여기서, 발효 흑마늘은 0.01 ~ 1mm의 입경을 갖는 분말로 분쇄하는 것이 바람직하며, 식감을 위하여 3 ~ 10mm로 세절될 수 있음을 미리 밝혀둔다.Here, the fermented black garlic is preferably pulverized into a powder having a particle size of 0.01 to 1 mm, and it is disclosed in advance that it can be cut into 3 to 10 mm for texture.
이상과 같은 공정을 통해 만들어진 발효 흑마늘은 전통방식에 의해 제조되는 흑마늘과 대비할 때 다음과 같은 특징이 있다.The fermented black garlic produced through the above process has the following characteristics in comparison with the black garlic produced by the conventional method.
첫째, 단시간 및 적은 공정으로도 발효 흑마늘의 제조가 가능하다.First, it is possible to manufacture fermented black garlic in a short time and with a few steps.
둘째, 유산균 생산물질의 낮은 pH에 의해 잡균의 번식을 원천적으로 차단할 수 있다.Second, the low pH of the lactic acid producing material can prevent the propagation of the germs.
셋째, 특정 유산균 및 그의 유산균 생성물질에 의한 발효를 통하여 발효 흑마늘의 품질 관리가 용이하다.Third, quality control of fermented black garlic is facilitated through fermentation by a specific lactic acid bacterium and its lactic acid bacterial producing material.
넷째, 증숙하거나 구워서 흑마늘을 만드는 종래의 흑마늘 제조방식은 마늘의 유효한 성분을 유출시키거나 파괴되는 것에 비해 유산균 생산물질에 의한 발효 흑마늘을 유산균에 의한 선택적 발효가 가능한 장점이 있다.Fourth, the conventional method of producing black garlic by boiling or baking is advantageous in that fermented black garlic produced by the lactic acid producing material can be selectively fermented by lactic acid bacteria, as compared with a case where effective ingredients of garlic are leached or destroyed.
2. 콩 요거트 제조하기 2. Making Soy Yogurt
a. 콩 세척 및 건조a. Washing and drying beans
생콩을 5 ~ 30℃의 정제수를 이용하여 적어도 1회 이상 세척하여 표면의 이물질을 제거하되, 수용성 영양성분의 유출을 방지하기 위하여 30분 이내로 세척한 후 10 ~ 30℃의 저온 냉풍으로 건조하거나 자연 건조한다.The beans are washed at least once with purified water at 5 to 30 ° C to remove foreign substances on the surface. To prevent leakage of the water-soluble nutrients, the beans are washed within 30 minutes and then dried with cold air at 10 to 30 ° C, Dry.
본 실시예에 사용되는 생콩은 특정 품종에 한정되지 않으며 특정 품종 하나를 단독으로 사용하거나 또는 여러 품종을 혼합하여 사용될 수 있다.The soybean cakes used in this embodiment are not limited to specific varieties, and may be used singly or in combination of several varieties.
b. 분쇄b. smash
건조된 생콩을 분쇄기를 이용하여 80 ~ 170㎛의 입경(particle size)을 갖도록 분쇄한다. 여기서, 입경을 80 ~ 170㎛으로 유지하는 이유는 후술되는 발효과정을 최적화하기 위함이다.The dried soybean cake is pulverized using a pulverizer to have a particle size of 80 to 170 mu m. Here, the reason why the grain size is kept at 80 to 170 탆 is to optimize the fermentation process described later.
c. 배합 및 살균c. Compounding and sterilization
물 100 중량부에 대하여 생콩가루 30 ~ 50 중량부를 혼합한 두유액을 90 ~ 120℃에서 40 ~ 90분간 끓여 살균한다.30 to 50 parts by weight of raw soy flour mixed with 100 parts by weight of water is boiled for 40 to 90 minutes at 90 to 120 ° C for sterilization.
d. 두유액 발효 및 콩 요거트 제조d. Soy milk liquid fermentation and soy yoghurt production
두유액을(고형물 혼재) 40 ~ 45℃로 냉각한 후, 두유액 100 중량부에 대하여 유산균 3 ~ 10 중량부를 접종시켜 6 ~ 10시간 정치시켜 발효하여 콩 요거트를 제조한다.After the soymilk solution is cooled to 40 to 45 占 폚, 3 to 10 parts by weight of lactic acid bacteria are inoculated per 100 parts by weight of the soy milk solution, and the mixture is allowed to stand for 6 to 10 hours to ferment the soybean yogurt.
본 실시예에 적용되는 유산균은 대한민국 식품의약품안전처가 기능성 원재료로 인정하고 있는 락토바실루스, 락토코커스, 엔테로코커스, 스트렙토코커스 및 비피도박테리움 등 5가지 속에 포함되는 19종 중 1종 이상으로, 락토바실루스 속에는 아시도필루스, 카세이, 가쎄리, 불가리쿠스, 헬베티쿠스, 퍼멘텀, 파라카세이, 플란타럼, 루테리, 람노서스, 살리바리우스 등의 11종이 포함되고, 락토코커스 속에는 락티스 1종이 포함되고, 엔테로코커스 속에는 페시움, 페칼리스 등의 2종이 포함되고, 스트렙토코커스 속에는 써모필루스 1종이 포함되고, 비피도박테리움 속에는 비피덤, 브레베, 롱검, 락티스 등의 4종이 포함된다.The lactic acid bacteria to be used in the present embodiment are at least one of 19 kinds contained in five genera, Lactobacillus, Lactococcus, Enterococcus, Streptococcus and Bifidobacterium, which are recognized as functional raw materials by the Korea Food & Drug Administration Bacillus contains 11 species including Ashidopilus, Kasei, Gasseri, Bulgarian Kus, Helveticus, Fermestum, Paracasei, Planta Rum, Luteri, Lambrosus and Salivarius. Lactococcus contains 11 species of Lactis In the Enterococcus, there are two species such as Pesium and Pecalis. Streptococcus contains one species of Thermophilus. Bifidobacterium contains four species such as Bifidum, Breve, Longtom and Lactis. .
3. 발효 흑마늘 분말이 첨가된 요거트 제조 3. Manufacture of yoghurt with added fermented black garlic powder
콩 요거트의 pH가 4.0 ~ 4.5일 때 콩 요거트 100 부피%에 대하여 발효 흑마늘 분말을 5 ~ 20 부피%를 첨가한 후 교반한 다음, 2 ~ 5℃로 급속냉각하여 포장한다.When the pH of soybean yogurt is 4.0 ~ 4.5, 5 ~ 20% by volume of fermented black garlic powder is added to 100% by volume of soy yoghurt, and the mixture is stirred at 2 ~ 5 ℃ for rapid cooling.
이상과 같이 최종 제조된 요거트에는 BCP 첨가 평판 측정용 배지를 활용하여 측정한 결과, g당 5.5억마리의 유산균이 함유되어 있음을 확인할 수 있었다.The yogurt thus prepared was found to contain about 550 million lactic acid bacteria per gram by using a medium for measuring a flat plate containing BCP.
그리고 풍미를 개선하기 위하여, 콩 요거트 100 부피%에 대하여 솔잎 혼합 발효액을 0.1 ~ 0.3 부피%를 더 첨가할 수 있다.In order to improve the flavor, 0.1 to 0.3% by volume of pine needle mixed fermentation broth can be further added to 100% by volume of soy yoghurt.
송진가루 혼합 발효액은 다음과 같은 제조공정에 의해 제조된다.The mixed powder of rosin powder is prepared by the following manufacturing process.
① 송진가루 100 중량부에 대하여, 말린 솔잎 40 ~ 50 중량부, 구연산 0.1 ~ 0.3 중량부 및 설탕 30 ~ 35 중량부를 충전한 후 5 ~ 10℃에서 12 ~16개월 발효시킨 다음 여과한다.(1) Charged with 40 to 50 parts by weight of dried pine leaves, 0.1 to 0.3 parts by weight of citric acid and 30 to 35 parts by weight of sugar, and then fermented at 5 to 10 ° C for 12 to 16 months, and then filtered.
② 정제수 100 ~ 500 중량부 및 천일염 4 ~ 5 중량부를 더 첨가한 후 80 ~ 100℃ 온도에서 5 ~ 10분간 끓인 다음 냉각한 다음 여과하여 제조된다.(Ii) adding 100 to 500 parts by weight of purified water and 4 to 5 parts by weight of a salt of the sun, boiling at a temperature of 80 to 100 DEG C for 5 to 10 minutes, cooling and then filtering.
다양한 variety 실시예들Examples
(가) 장기간 보관이 가능한 실시예(A) Examples in which long-term storage is possible
콩 요거트의 pH가 4.0 ~ 4.5일 때 콩 요거트 100 부피%에 대하여 발효 흑마늘 분말 5 ~ 20 부피%를 첨가한 후 교반하는 발효 흑마늘이 첨가된 요거트 제조공정에서, 급속냉각하여 포장하지 않고 살균한 다음 액상 제품화하면 보관성이 증가된다.When the pH of soybean yoghurt is 4.0 ~ 4.5, 5 ~ 20% by volume of fermented black garlic powder is added to 100% by volume of soy yoghurt and then fermented black garlic is added, followed by rapid cooling and sterilization without packaging Liquid commercialization increases storage.
(나) 편의성을 제공하는 실시예(B) Examples that provide convenience
콩 요거트의 pH가 4.0 ~ 4.5일 때 콩 요거트 100 부피%에 대하여 발효 흑마늘 분말 5 ~ 20 부피%를 첨가한 후 교반하는 발효 흑마늘이 첨가된 요거트 제조공정에서, 급속냉각한 다음 스프레이 건조방식, 냉동진공 건조방식 또는 열풍 건조방식 등을 활용하여 분말로 만들어 개별포장하면 보관이나 지참이 용이하게 된다.When the pH of soybean yoghurt is 4.0 ~ 4.5, 5 ~ 20% by volume of fermented black garlic powder is added to 100% by volume of soy yoghurt and then fermented black garlic is added. Vacuum drying method or hot air drying method can be used to make powder and make it easier to keep and bring it individually.
이상과 같은 본 발명은 일 실시예에 한정되어 설명되었으나, 이에 한정되지 않고 본 발명의 기술적 사상을 토대로 변형되는 실시예들은 모두 본 발명의 권리범위에 속함이 분명하다.While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments.
Claims (5)
(b) 두유액 100 중량부에 대하여 유산균 3 ~ 10 중량부를 접종시켜 6 ~ 10시간 정치시켜 발효하여 콩 요거트를 제조하는 단계와;
(c) 상기 콩 요거트의 pH가 4.0 ~ 4.5일 때 상기 (b)단계의 콩 요거트 100 부피%에 대하여 상기 발효 흑마늘 분말 5 ~ 20 부피% 및 송진가루 혼합 발효액 0.1 ~ 0.3 부피%를 첨가한 후 교반한 다음, 2 ~ 5℃로 급속냉각하는 단계로 이루어지되,
상기 송진가루 혼합 발효액은,
(차) 송진가루 100 중량부에 대하여, 말린 솔잎 40 ~ 50 중량부, 구연산 0.1 ~ 0.3 중량부 및 설탕 30 ~ 35 중량부를 충전한 후 5 ~ 10℃에서 12 ~16개월 발효시킨 다음 여과하는 단계와;
(카) 정제수 100 ~ 500 중량부 및 천일염 4 ~ 5 중량부를 더 첨가한 후 80 ~ 100℃ 온도에서 5 ~ 10분간 끓인 다음 냉각한 다음 여과하여 제조되는 단계로 제조되는 것을 특징으로 하는 발효 흑마늘을 이용한 콩 요거트 제조방법.
(a) immersing raw garlic in a lactobacillus-producing material to produce fermented black garlic, and then pulverizing to produce fermented black garlic powder;
(b) inoculating 3 to 10 parts by weight of lactic acid bacterium to 100 parts by weight of soybean milk, fermenting the soybean milk at a temperature of 6 to 10 hours to produce soybean yogurt;
(c) 5 to 20% by volume of the fermented black garlic powder and 0.1 to 0.3% by volume of the mixed powder of the rosin powder are added to 100% by volume of the soybean yoghurt in the step (b) when the pH of the soybean yogurt is 4.0 to 4.5 Followed by rapid cooling to 2 to 5 DEG C,
The rosin mixture fermentation broth may contain,
(Tea) To 40 to 50 parts by weight of dried pine leaves, 0.1 to 0.3 parts by weight of citric acid and 30 to 35 parts by weight of sugar, and fermenting the mixture at 5 to 10 ° C for 12 to 16 months, Wow;
(K) 100 to 500 parts by weight of purified water and 4 to 5 parts by weight of a salt of the sun, and then boiling at a temperature of 80 to 100 ° C for 5 to 10 minutes, followed by cooling and filtration. Method of manufacturing soybean yogurt using.
상기 (a)단계는,
(가) 생마늘을 5 ~ 30℃의 정제수를 이용하여 적어도 1회 이상 세척하여 표면의 이물질을 제거한 후 표피를 제거하는 단계와;
(나) 아미노산 및 유산균이 함유된 유산균 생성물질 100 부피%에 대하여 상기 세척된 생마늘을 30 ~ 50 부피% 비율로 침지하여 40 ~ 45℃에서 3 ~ 5일 동안 발효하여 발효 흑마늘을 제조하는 단계와;
(다) 상기 발효 흑마늘을 분쇄하여 분말로 만드는 단계로 이루어지되,
상기 (나)단계의 유산균은 락토바실루스 속에는 아시도필루스, 카세이, 가쎄리, 불가리쿠스, 헬베티쿠스, 퍼멘텀, 파라카세이, 플란타럼, 루테리, 람노서스, 살리바리우스가 포함되고, 락토코커스 속에는 락티스가 포함되고, 엔테로코커스 속에는 페시움, 페칼리스가 포함되고, 스트렙토코커스 속에는 써모필루스가 포함되고, 비피도박테리움 속에는 비피덤, 브레베, 롱검, 락티스 중 선택된 1종 이상인 것을 포함하고,
상기 아미노산은 발린, 루신, 아이소루이신, 메티오닌, 트레오닌, 라이신, 페닐알라닌, 트립토판, 히스티딘인 것을 특징으로 하는 발효 흑마늘을 이용한 콩 요거트 제조방법.
The method according to claim 1,
The step (a)
(A) washing raw garlic with purified water at 5 to 30 ° C at least once to remove foreign matter on the surface, and then removing the epidermis;
(B) fermenting the fermented black garlic by immersing the washed raw garlic in a proportion of 30 to 50% by volume with respect to 100% by volume of the lactic acid bacteria-producing material containing amino acid and lactic acid bacterium at 40 to 45 ° C for 3 to 5 days, ;
(C) pulverizing the fermented black garlic into powder,
The lactic acid bacteria in step (b) above include Lactobacillus such as Ashidopilus, Kasei, Gasseri, Bulgarian, Helveticus, Fermestum, Paracasei, Pteralum, Luteri, Lambsos, Salivarius, The Enterococcus includes Pethium, Pecalis, the Streptococcus, and the Bifidobacterium include one or more selected from Bifidum, Breve, Longham, and Lactis. ≪ / RTI >
Wherein the amino acid is valine, leucine, isosurine, methionine, threonine, lysine, phenylalanine, tryptophan, histidine.
상기 (b)단계의 두유액은,
(라) 생콩을 5 ~ 30℃의 정제수를 이용하여 적어도 1회 이상 세척하여 표면의 이물질을 제거하되, 수용성 영양성분의 유출을 방지하기 위하여 30분 이내로 세척한 후 10 ~ 30℃의 저온 냉풍으로 건조하거나 자연 건조하는 단계와;
(마) 건조된 생콩을 분쇄기를 이용하여 80 ~ 170㎛의 입경(particle size)을 갖도록 분쇄하여 생콩가루를 제조하는 단계와;
(바) 물 100 중량부에 대하여 상기 생콩가루 30 ~ 50 중량부를 혼합한 두유액을 90 ~ 120℃에서 40 ~ 90분간 끓여 살균하는 단계에 의해 제조되고,
상기 (b)단계의 유산균은 락토바실루스 속에는 아시도필루스, 카세이, 가쎄리, 불가리쿠스, 헬베티쿠스, 퍼멘텀, 파라카세이, 플란타럼, 루테리, 람노서스, 살리바리우스가 포함되고, 락토코커스 속에는 락티스가 포함되고, 엔테로코커스 속에는 페시움, 페칼리스가 포함되고, 스트렙토코커스 속에는 써모필루스가 포함되고, 비피도박테리움 속에는 비피덤, 브레베, 롱검, 락티스 중 선택된 1종 이상인 것을 특징으로 하는 발효 흑마늘을 이용한 콩 요거트 제조방법.
The method according to claim 1,
The soy milk solution in the step (b)
(D) The beans are washed at least once with purified water at 5 ~ 30 ℃ to remove the foreign substances on the surface. To prevent leakage of the water-soluble nutrients, the beans are washed within 30 minutes and then cooled at 10 ~ 30 ℃ Drying or naturally drying;
(E) pulverizing dried soybeans to have a particle size of 80 to 170 mu m using a pulverizer to prepare raw soy flour;
(F) 30 to 50 parts by weight of the raw soy flour is added to 100 parts by weight of water, and the resulting soy milk is boiled at 90 to 120 ° C. for 40 to 90 minutes,
The lactic acid bacterium of the step (b) includes ashidophilus, kasei, gasseri, bulgaricus, helveticus, perfumeum, paracasei, plastarum, luterium, lambsosus and salivarius in the genus Lactobacillus, The Enterococcus includes Pethium, Pecalis, the Streptococcus, and the Bifidobacterium include one or more selected from Bifidum, Breve, Longham, and Lactis. Wherein the fermented black garlic is used for producing soybean yoghurt.
상기 (나)단계에 상기 아미노산 및 유산균이 함유된 유산균 생성물질 100 부피%에 대하여 로스팅된 와송 분말을 1 ~ 2 부피%를 더 첨가하고,
상기 로스팅 와송 분말은,
(사) 꽃잎, 씨방, 잎 및 줄기를 모두 포함하여 건조된 와송을 60 ~ 100℃에서 5 ~ 10분간 증숙한 다음 35 ~ 40℃로 냉각하는 단계와;
(아) 상기 냉각된 와송을 1 ~ 2㎜의 입경을 갖도록 분쇄하여 와송 분말을 만드는 단계와;
(자) 180 ~ 230℃로 가열된 로스터에서 상기 와송 분말을 로스팅한 다음, 30 ~ 35℃로 급속냉각하여 제조되는 단계로 제조되는 것을 특징으로 하는 발효 흑마늘을 이용한 콩 요거트 제조방법.3. The method of claim 2,
In step (b), 1 to 2 volume% of rosin powder is further added to 100 volume% of the lactic acid bacteria-producing material containing the amino acid and the lactic acid bacterium,
The roasting powder,
(G) mixing the dried petioles, ovaries, leaves and stems together at 60 to 100 ° C for 5 to 10 minutes and cooling to 35 to 40 ° C;
(A) pulverizing the cooled pulp to have a particle diameter of 1 to 2 mm to produce a pulverized powder;
Wherein the roasted powder is roasted in a roaster heated at 180 to 230 ° C and then rapidly cooled to 30 to 35 ° C to produce soybean yoghurt using fermented black garlic.
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KR102396235B1 (en) * | 2021-12-08 | 2022-05-10 | 강보라 | Manufacturing method of greek yogurt using rhynchosia nulubilis |
KR102671869B1 (en) | 2023-11-06 | 2024-06-10 | 주식회사 정.식품 | Novel fermentation starter of Lacticaseibacillus paracasei subsp. paracasei DCF0420 and plant-based yogurt uses thereof |
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KR102396235B1 (en) * | 2021-12-08 | 2022-05-10 | 강보라 | Manufacturing method of greek yogurt using rhynchosia nulubilis |
KR102671869B1 (en) | 2023-11-06 | 2024-06-10 | 주식회사 정.식품 | Novel fermentation starter of Lacticaseibacillus paracasei subsp. paracasei DCF0420 and plant-based yogurt uses thereof |
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