CN103393094A - Preparation method of ganoderma lucidum mycelium slices - Google Patents
Preparation method of ganoderma lucidum mycelium slices Download PDFInfo
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Abstract
The invention provides a preparation method of ganoderma lucidum mycelium slices, which can be used for solving the technical problem that ganoderma lucidum mycelium powder is inconvenient to eat in the prior art. The preparation method comprises the steps of preparation of a culture medium, activation of strains, inoculation and culture, baking, puffing, crushing, mixing, granulating and tabletting, wherein the preparation of the culture medium comprises the steps of: A, preparation of a grain culture medium; B, preparation of a slant culture medium; C, preparation of a shake flask culture medium; D, preparation of a fermentation tank culture medium. The preparation method can be widely used in the processing of the ganoderma lucidum mycelium slices.
Description
Technical field
The present invention relates to a kind of preparation method of health products, be specifically related to a kind of preparation method of Ganoderma lucidum mycelium sheet.
Background technology
Glossy ganoderma has good medicine and health care and is worth, in China, nearly more than 2000 years of its medicinal history, modern study shows that glossy ganoderma has the various active composition, as GL-B, ganodenic acid, alkaloid, amino acid and various trace elements etc., have the plurality kinds of health care such as antitumor, anti-ageing, radiation proof, raising immunity and be worth, along with the development of glossy ganoderma seed selection and cultivation technique, Garnoderma product also becomes more diversified.Present stage, common glossy ganoderma correlated product mainly contained three kinds of ganoderma lucidum fruitbody goods, ganoderma spore powder product and Ganoderma lucidum mycelium powder products.And Ganoderma lucidum mycelium powder because its processing method has advantages of that operation requirements is lower, output is high, product active ingredient content is higher than fructification, easily realize industrialization etc., the occupation rate of market of product is the highest.
Ganoderma lucidum mycelium powder be Ganoderma lucidum mycelium that the lucidum strain fermented cultivation of high-quality is obtained expanded and pulverize after obtain, active component content is higher than ganoderma lucidum fruitbody,, by adding trace element as inorganic selenium and inorganic Ge etc., can obtain rich selenium germanium glossy ganoderma hypha powder during the fermentation.
But, there is the material that triterpene etc. is insoluble to exist in Ganoderma lucidum mycelium powder, should not make electuary, and passing through of mentioning in other processing methods extracted and separates the method for carrying out deep processing, equipment requirement is high, and is not easy to operate, and easily causes the loss of Ganoderma lucidum mycelium powder active ingredient.
Summary of the invention
The present invention is exactly in order to solve the technical problem of the Ganoderma lucidum mycelium powder inconvenient eating that exists in prior art, and the preparation method of the Ganoderma lucidum mycelium sheet of a kind of instant, comprehensive nutrition is provided, and comprises the following steps:
A: the preparation of grain culture medium
Wheat, corn, millet, soya bean are pressed ratio of quality and the number of copies 1:1.5～2:0.6～1:1～1.2, add water to water content mass percent 35～40%, divide and be filled in fermentation flask, then carry out high-temperature sterilization, sterilising conditions: 121～123 ℃, 40～60min; After sterilizing end, when the culture medium temperature is down to 27～35 ℃, puts into the aseptic inoculation chamber and treat inoculation use;
B: the preparation of slant medium
According to mass percent glucose 15～20%, analysis for soybean powder 15～17%, corn flour 10～12%, wheat flour 10～13%, potassium dihydrogen phosphate 0.3～0.8%, magnesium sulfate 0.2～1%, agar 3～5%, peptone 3～7%, remaining be the proportional arrangement of the sweet potato extraction solution, pH transfers to 7.0, divide and be filled in test tube, mix rear high-temperature sterilization, sterilising conditions: 121～123 ℃, 40～60min; Take out test tube after sterilizing end 10～15min, be put into inclined-plane, slant medium is transferred to constant temperature culture 18～20h in 28～36 ℃ of incubators, without bacterium colony grow can take out standby;
C: the preparation of shaking flask culture medium
According to mass percent glucose 16～21%, analysis for soybean powder 15～17%, corn flour 10～12%, wheat flour 10～13%, potassium dihydrogen phosphate 0.3～0.8%, magnesium sulfate 0.2～1%, peptone 3～7%, remaining be the proportional arrangement of the sweet potato extraction solution, pH6.8～7.2, mix rear high-temperature sterilization, sterilising conditions: 121～123 ℃, 40～60min;
D: the preparation of fermentation tank culture medium
proportioning according to analysis for soybean powder 450～500g/kg, starch 250～300g/kg, glucose 100～180g/kg, potassium dihydrogen phosphate 5～10g/kg, magnesium sulfate 2～8g/kg, peptone 30～70g/kg is prepared, analysis for soybean powder and the dilution that is dissolved in water after albumen powder mixes, add successively glucose after being heated to 60～70 ℃, potassium dihydrogen phosphate, magnesium sulfate and peptone, mixed liquor after mixing is fully joined in retort, continuation is diluted with water to 1000L to mixed liquor, retort is heated, when rising to 100～105 ℃, temperature carries out an exhaust, while continuing to be warming up to 121～123 ℃, close respirator and import and export switch, make mixed culture medium at 121～123 ℃, 0.15 sterilizing 30min in～0.17MPa environment, sterilizing complete, closing heating system makes mixed culture medium be cooled to 25～28 ℃ of heat preservation for standby use,
The preparation process of the sweet potato extraction solution comprises: Ipomoea batatas is cleaned peeling, be put in digester and use poach, Ipomoea batatas to boil to erosion, use filtered through gauze, get its filtrate, be the sweet potato extraction solution;
The activated spawn step comprises:
(1) select the red sesame of On Polyporaceae, be inoculated into slant medium and cultivate, condition of culture is: 26～30 ℃, 2～3 days, treat that the inclined-plane mycelia covers with, namely as mass-produced female a that plants;
(2) select head mold, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female b that plants;
(3) select ascus Cordycepps saccharomycete, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female c that plants;
(4) get above-mentionedly female plant a, b, c bacterium piece is seeded to the shaking flask culture medium according to the ratio of mass ratio 1:2:1.5,26～30 ℃ are cultured to the bacterium block length and insert shaking table after circular, 28～30 ℃ of cultivation 60～70h of constant temperature, namely obtain the Liquid Culture bacterial classification after having mycelium pellet to form;
In inoculation and incubation step, the Liquid Culture bacterial classification is inoculated in the grain culture medium that has prepared, 25～30 ℃ of lower lucifuges, cultivated 16～22 days;
In blend step, according to the ratio of mixture gross mass 1.5～2%, add antioxidant in Ganoderma lucidum mycelium powder, stir and mix;
In granulation step, adding respectively mass concentration according to the ratio of mixture total weight amount 10～20% is that 70% wetting agent solution and mass fraction are 5～20% binder solution, stir 10～25min in mixer, then cross 8～20 mesh sieves, obtain graininess Ganoderma lucidum mycelium powder product;
In the compressing tablet step, add the Ganoderma lucidum mycelium powder particles and weigh 2～5% disintegrant and mix; Select rotary tablet machine to carry out compressing tablet to graininess glossy ganoderma powder goods, control strip is heavily 0.2～0.35g, and sheet thickness is 0.13～0.2mm.
Preferably, in blend step, the antioxidant that uses is calcium lactate.
Preferably, in granulation step, the wetting agent that uses is absolute ethyl alcohol.
Preferably, in granulation step, the adhesive that uses is gelatinized corn starch.
Preferably, in the compressing tablet step, disintegrant used is low-substituted hydroxypropyl cellulose.
Mix: the ratio according to mixture gross mass 0.5-2% adds antioxidant in Ganoderma lucidum mycelium powder, stir and mix;
The invention has the advantages that:
1, the composition of multiple easy oxidized decomposition is arranged in Ganoderma lucidum mycelium powder, make tablet, can effectively protect these easily oxidized compositions, and tablet is edible and carry convenient;
2, after the good water solubility of Ganoderma lucidum mycelium powder and dissolving, the viscosity small flow is strong, should not adopt water to make wetting agent, consider simultaneously the water-insoluble of triterpene substance, select absolute ethyl alcohol as wetting agent in wet-granulation process, this also is conducive to improve dissolving and the absorptivity of triterpene substance;
3, use the starch slurry adhesive to have a lot of advantages, reason has: 1. the viscosity of Ganoderma lucidum mycelium powder itself is less, should select the higher adhesive of viscosity; 2. starch slurry wetting material equably, be not prone to local excessively wet phenomenon, and good adhesive effect arranged, and is to apply adhesive more widely; 3. the source of starch slurry is wide, and cost is low, handled easily; 4. the taste of starch slurry is superficial, can not cover the original taste of Ganoderma lucidum mycelium powder;
4, easily oxidized lipid material and triterpenes components are arranged in Ganoderma lucidum mycelium powder, very easily oxidizedly not only can cause the generation bad smell that becomes sour in processing and storage, also can cause the loss of nutritional labeling, add calcium lactate protected in process, when playing antioxidation, can alleviate the sour and astringent sense of hypha powder;
5, adopt first the bacterial classification Mixed culture such as glossy ganoderma, head mold, saccharomycete, the bacterial classification survival rate is high, high, the anti-miscellaneous bacteria ability of mycelia quality is strong.
The specific embodiment
Bacterial classification source in following examples is as follows: the red sesame of On Polyporaceae (Ganoderma luncidum), and Yutai County, Shandong Province microorganism edible mushroom research institute makes;
Select head mold (koji), (Angel Yeast Co.,Ltd's manufacturing);
Ascus Cordycepps saccharomycete (brewer's yeast), (Angel Yeast Co.,Ltd's manufacturing).
Embodiment 1
1, the preparation of culture medium
(1) preparation of grain culture medium
After 100kg wheat, corn, millet, soya bean are carried out attrition crushing by the mass ratio of 1:1.5:0.6:1 and mixing, add water to water content 37%, divide and be filled in fermentation flask, then carry out high-temperature sterilization, sterilising conditions: 121 ℃, 40min, when the culture medium temperature is down to 27 ℃, puts into the aseptic inoculation chamber and treat inoculation use after sterilizing end;
(2) preparation of slant medium
The preparation of the sweet potato extraction solution: Ipomoea batatas is cleaned peeling, according to the ratio of Ipomoea batatas: water=1:0.8, join in digester, boil rear little fire and keep little boiling 15 minutes, Ipomoea batatas is boiled to erosion, crosses 4 layers of filtered through gauze, gets its filtrate, is the sweet potato extraction solution.As follows.
According to mass percent glucose 15%, analysis for soybean powder 15%, corn flour 10%, wheat flour 13%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 1%, agar 5%, peptone 7%, remaining be the proportional arrangement of the sweet potato extraction solution, pH7.0, divide and be filled in test tube, mix rear high-temperature sterilization, sterilising conditions: 121 ℃, 40min; Take out test tube after sterilizing end 10min, be put into while hot inclined-plane, slant medium is transferred to constant temperature culture 18h in 36 ℃ of incubators, without bacterium colony grow can take out standby;
(3) preparation of shaking flask culture medium
According to mass percent glucose 16%, analysis for soybean powder 36%, corn flour 10%, wheat flour 10%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.5%, peptone 7%, remaining be the proportional arrangement of the sweet potato extraction solution, pH6.8, mix rear high-temperature sterilization, sterilising conditions: 121 ℃, 40min;
(4) preparation of fermentation tank culture medium
According to analysis for soybean powder 475g/kg, starch 300g/kg, glucose 150g/kg, potassium dihydrogen phosphate 10g/kg, magnesium sulfate 5g/kg, the proportioning of peptone 70g/kg is prepared; Analysis for soybean powder and the dilution that is dissolved in water after albumen powder mixes, add successively glucose, potassium dihydrogen phosphate, magnesium sulfate and peptone after being heated to 60 ℃, mixed liquor after mixing is fully joined in retort, continuation is diluted with water to 1000L to mixed liquor, retort is heated, when rising to 100 ℃, temperature carries out an exhaust, close respirator while continuing to be warming up to 121 ℃ and import and export switch, make mixed culture medium sterilizing 30min in 121 ℃, 0.15MPa environment, sterilizing complete, close heating system and make mixed culture medium be cooled to 26 ℃ of heat preservation for standby use;
2, the activation of bacterial classification
(1) select the red sesame of On Polyporaceae, be inoculated into slant medium and cultivate, condition of culture is: 26～30 ℃, 2～3 days, treat that the inclined-plane mycelia covers with, namely as mass-produced female a that plants;
(2) select head mold, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female b that plants;
(3) select ascus Cordycepps saccharomycete, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female c that plants;
(4) get above-mentionedly female plant a, b, c bacterium piece is seeded to the shaking flask culture medium according to the ratio of mass ratio 1:2:1.5,26～30 ℃ are cultured to the bacterium block length and insert shaking table after circular, 28～30 ℃ of cultivation 60～70h of constant temperature, namely obtain the Liquid Culture bacterial classification after having mycelium pellet to form;
3, inoculation and cultivation
The Liquid Culture bacterial classification is inoculated in the grain culture medium that has prepared, 27 ℃ of lower lucifuges, cultivated 16 days;
4, oven dry
Cultured mycelium is dug out from solid medium, utilizes air drier to carry out drying and processing to it, to moisture lower than 2% only;
5, expanded
Mycelia after oven dry is inserted in mixing and blending machine and adds water and stir, carry out expandedly when water content reaches 16%, swelling temperature is 116 ℃;
6, pulverize
With micronizer, expanded compound hypha powder is pulverized, obtaining particle diameter is 200 purpose Ganoderma lucidum mycelium Ultramicro-powders; Ganoderma lucidum mycelium powder 10kg, add calcium lactate 60g, adding successively the 15kg mass concentration after stirring and mixing is that 70% ethanolic solution and 200g mass concentration are 18% gelatinized corn starch solution, stirs 18min in mixer, then cross 15 mesh sieves, obtain graininess Ganoderma lucidum mycelium powder product; With the ZP35A rotary tablet machine, graininess glossy ganoderma powder goods are carried out compressing tablet after adding the 5g low-substituted hydroxypropyl cellulose in the Ganoderma lucidum mycelium powder particles, during compressing tablet, control strip is heavily 0.2g, and sheet thickness is 0.15mm.
Embodiment 2
1, the preparation of culture medium
(1) preparation of grain culture medium
After 100kg wheat, corn, millet, soya bean are carried out attrition crushing by the mass ratio of 1:1.8:0.8:1.1 and mixing, add water to water content 38%, divide and be filled in fermentation flask, then carry out high-temperature sterilization, sterilising conditions: 121 ℃, 40min, when the culture medium temperature is down to 30 ℃, puts into the aseptic inoculation chamber and treat inoculation use after sterilizing end;
(2) preparation of slant medium
By mass percentage glucose 20%, analysis for soybean powder 40%, corn flour 11%, wheat flour 12%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.2%, agar 3%, peptone 6.5%, remaining be the proportional arrangement of the sweet potato extraction solution, pH7.0, divide and be filled in test tube, mix rear high-temperature sterilization, sterilising conditions: 122 ℃, 50min; Take out test tube after sterilizing end 13min, be put into while hot inclined-plane, slant medium is transferred to constant temperature culture 19h in 32 ℃ of incubators, without bacterium colony grow can take out standby;
(3) preparation of shaking flask culture medium
According to mass percent glucose 21%, corn flour 11%, wheat flour 12%, analysis for soybean powder 40%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.7%, peptone 3%, remaining be the proportional arrangement of the sweet potato extraction solution, pH7.0, mix rear high-temperature sterilization, sterilising conditions: 122 ℃, 50min;
(4) preparation of fermentation tank culture medium
According to analysis for soybean powder 450g/kg, starch 300g/kg, glucose 180g/kg, potassium dihydrogen phosphate 6g/kg, magnesium sulfate 4g/kg, the proportioning of peptone 70g/kg is prepared; Analysis for soybean powder and the dilution that is dissolved in water after albumen powder mixes, add successively glucose, potassium dihydrogen phosphate, magnesium sulfate and peptone after being heated to 65 ℃, mixed liquor after mixing is fully joined in retort, continuation is diluted with water to 1000L to mixed liquor, retort is heated, when rising to 103 ℃, temperature carries out an exhaust, close respirator while continuing to be warming up to 122 ℃ and import and export switch, make mixed culture medium sterilizing 30min in 122 ℃, 0.16MPa environment, sterilizing complete, close heating system and make mixed culture medium be cooled to 27 ℃ of heat preservation for standby use;
2, the activation of bacterial classification
(1) select the red sesame of On Polyporaceae, be inoculated into slant medium and cultivate, condition of culture is: 26～30 ℃, 2～3 days, treat that the inclined-plane mycelia covers with, namely as mass-produced female a that plants;
(2) select head mold, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female b that plants;
(3) select ascus Cordycepps saccharomycete, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female c that plants;
(4) get above-mentionedly female plant a, b, c bacterium piece is seeded to the shaking flask culture medium according to the ratio of mass ratio 1:2:1.5,26～30 ℃ are cultured to the bacterium block length and insert shaking table after circular, 28～30 ℃ of cultivation 60～70h of constant temperature, namely obtain the Liquid Culture bacterial classification after having mycelium pellet to form;
3, inoculation and cultivation
The Liquid Culture bacterial classification is inoculated in the grain culture medium that has prepared, 28 ℃ of lower lucifuges, cultivated 19 days;
4, oven dry
Cultured mycelium is dug out from solid medium, utilizes air drier to carry out drying and processing to it, to moisture lower than 2% only;
5, expanded
Mycelia after oven dry is inserted in mixing and blending machine and adds water and stir, carry out expandedly when water content reaches 17%, swelling temperature is 115 ℃;
6, pulverize
With micronizer, expanded compound hypha powder is pulverized, obtaining particle diameter is 200 purpose Ganoderma lucidum mycelium Ultramicro-powders;
Ganoderma lucidum mycelium powder 10kg, add antioxidant 80g, adding successively the 10kg mass concentration after stirring and mixing is that 70% ethanolic solution and 200g mass concentration are 15% gelatinized corn starch solution, stirs 25min in mixer, then cross 20 mesh sieves, obtain graininess Ganoderma lucidum mycelium powder product; With the ZP35A rotary tablet machine, graininess glossy ganoderma powder goods are carried out compressing tablet after adding the 5g low-substituted hydroxypropyl cellulose in the Ganoderma lucidum mycelium powder particles, during compressing tablet, control strip is heavily 0.25g, and sheet thickness is 0.18mm.
Embodiment 3
1, the preparation of culture medium
(1) preparation of grain culture medium
After 100kg wheat, corn, millet, soya bean are carried out attrition crushing by the mass ratio of 1:2:1:1.2 and mixing, add water to water content 40%, divide and be filled in fermentation flask, then carry out high-temperature sterilization, sterilising conditions: 123 ℃, 60min, when the culture medium temperature is down to 35 ℃, puts into the aseptic inoculation chamber and treat inoculation use after sterilizing end;
(2) preparation of slant medium
According to mass percent glucose 18%, corn flour 12%, wheat flour 13%, analysis for soybean powder 38%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.2%, agar 4%, peptone 7%, remaining be the proportional arrangement of the sweet potato extraction solution, pH7.0, divide and be filled in test tube, mix rear high-temperature sterilization, sterilising conditions: 123 ℃, 60min; Take out test tube after sterilizing end 15min, be put into while hot inclined-plane, slant medium is transferred to constant temperature culture 20h in 36 ℃ of incubators, without bacterium colony grow can take out standby;
(3) preparation of shaking flask culture medium
According to mass percent glucose 20%, corn flour 12%, wheat flour 13%, analysis for soybean powder 38%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.2%, peptone 3%, remaining be the ratio preparation of the sweet potato extraction solution, pH7.2, mix rear high-temperature sterilization, sterilising conditions: 123 ℃, 60min;
(4) preparation of fermentation tank culture medium
According to analysis for soybean powder 500g/kg, starch 270g/kg, glucose 180g/kg, potassium dihydrogen phosphate 10g/kg, magnesium sulfate 5g/kg, the proportioning of peptone 35g/kg is prepared; Analysis for soybean powder and the dilution that is dissolved in water after albumen powder mixes, add successively glucose, potassium dihydrogen phosphate, magnesium sulfate and peptone after being heated to 70 ℃, mixed liquor after mixing is fully joined in retort, continuation is diluted with water to 1000L to mixed liquor, retort is heated, when rising to 105 ℃, temperature carries out an exhaust, close respirator while continuing to be warming up to 123 ℃ and import and export switch, make mixed culture medium sterilizing 30min in 123 ℃, 0.17MPa environment, sterilizing complete, close heating system and make mixed culture medium be cooled to 28 ℃ of heat preservation for standby use;
2, the activation of bacterial classification
(1) select the red sesame of On Polyporaceae, be inoculated into slant medium and cultivate, condition of culture is: 26～30 ℃, 2～3 days, treat that the inclined-plane mycelia covers with, namely as mass-produced female a that plants;
(2) select head mold, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female b that plants;
(3) select ascus Cordycepps saccharomycete, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female c that plants;
(4) get above-mentionedly female plant a, b, c bacterium piece is seeded to the shaking flask culture medium according to the ratio of mass ratio 1:2:1.5,26～30 ℃ are cultured to the bacterium block length and insert shaking table after circular, 28～30 ℃ of cultivation 60～70h of constant temperature, namely obtain the Liquid Culture bacterial classification after having mycelium pellet to form;
3, inoculation and cultivation
The Liquid Culture bacterial classification is inoculated in the grain culture medium that has prepared, 30 ℃ of lower lucifuges, cultivates 16-22 days;
4, oven dry
Cultured mycelium is dug out from solid medium, utilizes air drier to carry out drying and processing to it, to moisture lower than 2% only;
5, expanded
Mycelia after oven dry is inserted in mixing and blending machine and adds water and stir, carry out expandedly when water content reaches 20%, swelling temperature is 125 ℃;
6, pulverize
With micronizer, expanded compound hypha powder is pulverized, obtaining particle diameter is 200 purpose Ganoderma lucidum mycelium Ultramicro-powders;
Ganoderma lucidum mycelium powder 10kg, add antioxidant 200g, adding successively the 10kg mass concentration after stirring and mixing is that 70% ethanolic solution and 200g mass concentration are 20% gelatinized corn starch solution, stirs 10min in mixer, then cross 8 mesh sieves, obtain graininess Ganoderma lucidum mycelium powder product; With the ZP35A rotary tablet machine, graininess glossy ganoderma powder goods are carried out compressing tablet after adding the 5g low-substituted hydroxypropyl cellulose in the Ganoderma lucidum mycelium powder particles, during compressing tablet, control strip is heavily 0.35g, and sheet thickness is 0.2mm.
Claims (5)
1. the production method of a Ganoderma lucidum mycelium sheet, it comprises the following steps: prepare culture medium, activated spawn, inoculation and cultivation, oven dry, expanded, pulverizing, mixing, granulation, compressing tablet, it is characterized in that, the described step for preparing culture medium comprises:
A: the preparation of grain culture medium
Wheat, corn, millet, soya bean are pressed ratio of quality and the number of copies 1:1.5～2:0.6～1:1～1.2, add water to water content mass percent 35～40%, divide and be filled in fermentation flask, then carry out high-temperature sterilization, sterilising conditions: 121～123 ℃, 40～60min; After sterilizing end, when the culture medium temperature is down to 27～35 ℃, puts into the aseptic inoculation chamber and treat inoculation use;
B: the preparation of slant medium
According to mass percent glucose 15～20%, analysis for soybean powder 15～17%, corn flour 10～12%, wheat flour 10～13%, potassium dihydrogen phosphate 0.3～0.8%, magnesium sulfate 0.2～1%, agar 3～5%, peptone 3～7%, remaining be the proportional arrangement of the sweet potato extraction solution, pH transfers to 7.0, divide and be filled in test tube, mix rear high-temperature sterilization, sterilising conditions: 121～123 ℃, 40～60min; Take out test tube after sterilizing end 10～15min, be put into inclined-plane, slant medium is transferred to constant temperature culture 18～20h in 28～36 ℃ of incubators, without bacterium colony grow can take out standby;
C: the preparation of shaking flask culture medium
According to mass percent glucose 16～21%, analysis for soybean powder 15～17%, corn flour 10～12%, wheat flour 10～13%, potassium dihydrogen phosphate 0.3～0.8%, magnesium sulfate 0.2～1%, peptone 3～7%, remaining be the proportional arrangement of the sweet potato extraction solution, pH6.8～7.2, mix rear high-temperature sterilization, sterilising conditions: 121～123 ℃, 40～60min;
D: the preparation of fermentation tank culture medium
proportioning according to analysis for soybean powder 450～500g/kg, starch 250～300g/kg, glucose 100～180g/kg, potassium dihydrogen phosphate 5～10g/kg, magnesium sulfate 2～8g/kg, peptone 30～70g/kg is prepared, analysis for soybean powder and the dilution that is dissolved in water after albumen powder mixes, add successively glucose after being heated to 60～70 ℃, potassium dihydrogen phosphate, magnesium sulfate and peptone, mixed liquor after mixing is fully joined in retort, continuation is diluted with water to 1000L to mixed liquor, retort is heated, when rising to 100～105 ℃, temperature carries out an exhaust, while continuing to be warming up to 121～123 ℃, close respirator and import and export switch, make mixed culture medium at 121～123 ℃, 0.15 sterilizing 30min in～0.17MPa environment, sterilizing complete, closing heating system makes mixed culture medium be cooled to 25～28 ℃ of heat preservation for standby use,
The preparation process of described the sweet potato extraction solution comprises: Ipomoea batatas is cleaned peeling, be put in digester and use poach, Ipomoea batatas to boil to erosion, use filtered through gauze, get its filtrate, be the sweet potato extraction solution;
Described activated spawn step comprises:
(1) select the red sesame of On Polyporaceae, be inoculated into slant medium and cultivate, condition of culture is: 26～30 ℃, 2～3 days, treat that the inclined-plane mycelia covers with, namely as mass-produced female a that plants;
(2) select head mold, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female b that plants;
(3) select ascus Cordycepps saccharomycete, be inoculated into slant medium and cultivate, condition of culture is: 28～30 ℃, 1～2 day, treat that the inclined-plane mycelia covers with, namely as mass-produced female c that plants;
(4) get above-mentionedly female plant a, b, c bacterium piece is seeded to the shaking flask culture medium according to the ratio of mass ratio 1:2:1.5,26～30 ℃ are cultured to the bacterium block length and insert shaking table after circular, 28～30 ℃ of cultivation 60～70h of constant temperature, namely obtain the Liquid Culture bacterial classification after having mycelium pellet to form;
In described inoculation and incubation step, the Liquid Culture bacterial classification is inoculated in the grain culture medium that has prepared, 25～30 ℃ of lower lucifuges, cultivated 16～22 days;
In described blend step, add antioxidant in Ganoderma lucidum mycelium powder, stir and mix;
In described granulation step, add respectively wetting agent solution and binder solution, stir in mixer, then sieve, obtain graininess Ganoderma lucidum mycelium powder product;
In described compressing tablet step, add disintegrant and mix, selecting rotary tablet machine to carry out compressing tablet to graininess glossy ganoderma powder goods.
2. the preparation method of Ganoderma lucidum mycelium sheet according to claim 1, is characterized in that, in described blend step, the antioxidant that uses is calcium lactate.
3. the preparation method of Ganoderma lucidum mycelium sheet according to claim 1, is characterized in that, in described granulation step, the wetting agent that uses is absolute ethyl alcohol.
4. the preparation method of Ganoderma lucidum mycelium sheet according to claim 1, is characterized in that, in described granulation step, the adhesive that uses is gelatinized corn starch.
5. the preparation method of Ganoderma lucidum mycelium sheet according to claim 1, is characterized in that, in described compressing tablet step, disintegrant used is low-substituted hydroxypropyl cellulose.
Priority Applications (1)
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| US11261420B2 (en) | 2016-03-01 | 2022-03-01 | The Fynder Group, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
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| US11272726B2 (en) | 2019-02-27 | 2022-03-15 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
| US11478007B2 (en) | 2019-02-27 | 2022-10-25 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
| US11432575B2 (en) | 2019-02-27 | 2022-09-06 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles and membrane bioreactor design |
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| US11718954B2 (en) | 2019-06-18 | 2023-08-08 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
| US11118305B2 (en) | 2019-06-18 | 2021-09-14 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
| CN113999777A (en) * | 2021-12-13 | 2022-02-01 | 东莞市英芝堂生物工程有限公司 | Ganoderma sinensis with high polysaccharide yield and fermentation production process thereof |
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