CN1204692A - Mixed fermentation technology for health-care nutritive food - Google Patents
Mixed fermentation technology for health-care nutritive food Download PDFInfo
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Abstract
A biologic fermentation technology for making high-nutrients health-care food is based on traditional Chinese medicine compatible theory that both edible and medical fungi are used, and includes such technological steps as selection of fungus, mixed inoculation, culture, multi-stage multiplying and fermenting to obtain raw liquid rich in high-nutrients myceliums having health-care function. After filter, concentration and addition of excipient and flavouring, health-care nutritive food can be made up, which can be aimed to curing different diseases such as hyperlipemia, high bood sugar, cardiovascular disease, etc.
Description
The invention belongs to biofermentation technique, specifically utilize the biofermentation technique manufacturing to have the health-care recovery function, have a kind of new process of production of high nutritive value food again.
Along with the develop rapidly of social productive forces, human civilization is also towards the high-stage development, and healthy and longevity becomes the topic that increasing people is concerned about.Think by traditional Chinese medicine reason: hundred illness finds its way in by the mouth, influence the people healthy with long-lived major cause be that food intake is unreasonable, curing the disease is not so good as scheduling theories such as dietotherapy not as diseases prevention, medication, be rooted in the hearts of the people.Make more people more and more pursue civilization, science and the reasonableness of diet.The diet civilization that thereupon grows up is from simple food variety development composite nutrient food, functional food, protective foods.Resembling " height contains silicon life egg " in fashion over the years, " 851 " or the like all is the sign of food service industry modern science and technology.People promptly shift to bioengineering field to the sight of watching attentively at the same time, Mycophyta food, and the pharmaceutical use that resembles the high nutritive value of auricularia auriculajudae, mushroom and effective ingredient thereof makes to be accelerated greatly as the development of functional food paces.It is more noticeable especially those itself to be that food is gone into the Mycophyta development research of herbal class again.As the CN85104712.A patent, and disclosed 92112908.4 application documents all are the achievements that fungal food with health role is studied.Up to the present, it is also at the early-stage to produce health-care nutritional food with the method for biological fermentation, be confined to process that existing fungi kind is inquired into one by one, the method of using also is zymotechnique commonly used for over ten years basically, and the synchronous development of its nutritive value and medicinal treatment and prophylactic effect also is in the primitive stage.At present also do not seen the trial that forms the plyability health-care nutritional food as compatibility of drugs, and equally there's sth behind all this can do just as the traditional Chinese medical theory of extensive knowledge and profound scholarship in this field.
The purpose of this invention is to provide a kind of method of utilizing biological fermentation and produce the novel process of health-care nutritional food, specifically provide a kind of novel process of compatibility fermentation, make every effort to make that the pharmaceutical use of health-care nutritional food obtains multiplication or reinforcement, thereby reach the purpose of the effect of excavating efficient resource.
Key of the present invention is the theory of herbal medicine compatibility is extended among the Mycophyta health-care nutritional food production technique.When having high nutritive value, Mycophyta food also has pharmaceutical use in various degree, the reason that this is paid attention to by the sanipratics expert just.These value often embody by its effective ingredient.And much the effective ingredient of fungies is that very difficult quenching by its sporophore taken out.As glossy ganoderma, Poria cocos, its sporophore quality is solid, and moisture content immerses difficulty, takes out whole effective ingredients even pulverizing also may not necessarily be quenched for particle.Add that the cultivation time is long, the cost height can be used it as common protective foods by ordinary person, have certain degree of difficulty.Can cultivate by fermentative processing and to be rich in mycelial stoste, not only can obtain a large amount of effective ingredients by suitability for industrialized production at an easy rate, and can reduce cost greatly, thereby open up a new way for health-care nutritional food.Particularly be mixed by the selection to different strain, fermentative processing again makes its effective ingredient be enhanced and improve at medicinal function aspect certain.On mixed fermentation ground simultaneously promptly, influence each other promotions, fusion of various mycelium makes mixed fermentation stoste highlight significantly at health-care effect, rehabilitation or prophylactic function aspect certain.This main body thinking of the present invention just.
Traditional technology of producing fungal fermented filtrate is cultivation and the amplification by strain improvement, liquid spawn, handles obtaining to be rich in mycelial stoste again by ferment tank.Again with stoste after filtration, concentrate, add suitably batching, the sterilization back obtains final nourishing function food.Improvement of the present invention just will be in the past single culture choose, become choosing of mixed bacterial classification, selected two or more edible bacterium or the medicinal bacterial classifications that compatibility relationship is arranged, or the bacterial classification edible, that pharmaceutical use has concurrently.In proportion two or more bacterial classification combined inoculations are being contained the shaking in the bottle of substratum, can finally make to be rich in by traditional operational path later on and mix mycelial stoste one until the nutritive food of producing the enriched health care function.
Further specify below in conjunction with given embodiment and the objective of the invention is how to realize:
Embodiment 1: the production technique of " three Siberian cocklebur cakes ".
Purpose is to provide a kind of high-nutrition food with health-care recovery function for hyperglycemia, hyperlipemic patients.
Specifically choose auricularia auriculajudae, mushroom and Poria cocos three bacterial classifications, be mixed strains by the conventional inoculum size of zymotechnique 1: 1: 2 than being seeded in to shake to cultivate in the bottle, processing condition are grasped: temperature 25-30 ℃, shaking table mixing parametric 150-180rpm (rev/min), time is 86-86 hour, the mixed bacterium of taking off from shaking table, entering seed tank culture with 10% inoculum size (mixed strains and seeding tank volumetric ratio) amplifies, cultivate to amplify the liquid spawn that obtains mixed bacterium through three grades, again liquid spawn is introduced substratum and in fermentor tank, ferment and finally obtain the stoste that is rich in the mixing mycelia.Effective ingredient is organic in three kinds of mycelium of process mixed fermentation merges together, has formed to have high nutritive value, and has had function magma hypoglycemic, blood fat reducing function.In above-mentioned zymotechnique, the major ingredient of substratum comprises Semen Maydis powder, flour, bean powder, a little sugar, yeast powder and inorganic salt, by C: N=(20-40): 1 principle is made into, the processing condition of fermentor tank are controlled at: 25 ℃-30 ℃ of temperature, pressure 0.05-0.08MPa, air flow 1: 0.6-1: 1, pH value is 4.5-6.5, mixing parametric 150-200rpm, fermentation time 40-60 hour.The three kinds of mycelial stostes of making by above technology that are rich in contain very high nutritive value and pharmaceutical use composition, stoste is concentrated the back add vehicle, here mainly be flour and rice meal, and required milk powder, fruit juice, salt compounded of iodine, egg and a spot of essence, sweet Stevia and an amount of sodium bicarbonate of biscuit is made in adding, finally make " three Siberian cocklebur cakes ", a kind of exactly have a clearly protective foods of hypoglycemic, lipid-lowering effect.Auricularia polycose in the fungus mycelium, the rich in amino acid in the mushroom mycelium has determined its high nutritive value, and the effective ingredient in the Poria cocos mycelia is the reason of Li Shui, hypoglycemic, fat-reducing effect, and black fungus itself also has the drug effect of lipopenicillinase and vasodilation in addition.The result of mixed fermentation has not still simplified production technique, reduced production cost, and make the effect between all kinds of mycelia complement each other, strengthen, make this functional food when having high edibleness, the effect that also has obvious treatment and prevent diabetes and hyperlipidaemia is this class patient ideal protective foods.Do clinic trial with this product and observe the patient of 30 routine different oedema and take " three Siberian cocklebur cakes " every day three times, each ten, serve on a week, interrupt other hydragog(ue) therebetween, 23 routine produce effects as a result, effective 7 examples.The organic oedema patient of the back proof of comparing than non-specific oedema patient act on obviously, rapid.Select diabetic subject's ten examples in addition, stop other ofhypoglycemic medicine, took continuously one month, every day, the 30-40 sheet wherein had 8 routine blood sugar obviously to reduce, and further control group evidence statistics is meaningful.Other case slightly.
Embodiment 2: " black sweet oral liquid " production technique
Purpose is to provide a kind of high-nutrition food with anti-bolt, restoring consciouness, hypotensive effect, provides a kind of new dietotherapy approach to health care brain and prevention brain disorder.
The selected bacterial classification of carrying is black fungus and honey mushroom in the present embodiment, and effective ingredient is an Auricularia polycose in the black fungus, removes outside high nourishing is worth, have stimulate the menstrual flow, invigorate blood circulation, antithrombotic effect.The similar rhizoma Gastrodiae of honey mushroom effective ingredient is effective conditioning agent of cranial nerve, and cerebral lesion is caused numbness of the limbs, and step-down has good effect.The both is a Mycophyta, can finish the production technique of mixed fermentation with it.In 1: 2 ratio black fungus and the combined inoculation of honey mushroom kind are cultivated in shaking bottle and to be mixed strains.Processing condition are 30 ℃-35 ℃ of temperature, and shaking table parameter 200-300rpm cultivated time 60-80 hour.With mixed strains by 10% inoculum size in seeding tank three grades amplify to cultivate the liquid spawns of making mixed bacterium, again liquid spawn is introduced the special culture media acquisition of in fermentor tank, ferment and is rich in two kinds of mycelial stostes.Mend brain for further improving it, invigorate blood circulation, the effect of anti-bolt, substratum in the technology of the present invention is taked special compound method: get 7.5% chrysanthemum, add and join 11.5% Vegetable Sponge of Luffa and decoct the base fluid make substratum, add by 30: 1 carbon-nitrogen ratio principle again and join Semen Maydis powder, wheat bran, bean powder, a little sugar, yeast and inorganic salt.Outside mycelial developmental process is removed and influenced each other like this, also equaled in chrysanthemum, Vegetable Sponge of Luffa soup, to cultivate, make its for invigorate blood circulation, the effect of anti-bolt, lipopenicillinase, step-down is further strengthened, and makes its function of health care more outstanding.Processing condition in the fermentor tank are: 30 ℃-35 ℃ of temperature, pressure 0.06-0.08MPa, air flow are 1: 1, mixing parametric 200-300rpm, pH value 6.7-7.0, fermentation time 35-45 hour.So can concentrate after the direct filtration after oral liquid makes stoste because this product is produced, adding is produced the required fruit juice of oral liquid, sucrose seasonings and a small amount of sanitas and is finished product.This product high nutritive value is its Auricularia polycose and rich in amino acid content, and its real medicinal health value just seems very outstanding.With dizziness, headache that this product treatment cerebral infarction sequela, cerebral apoplexy, cerebral blood supply insufficiency, hypertension cause, clinical average effectiveness level reaches more than 95%.Set up the control group (each 20 example experiment) that rat suppository forms model, thrombosis is prevented that effectively rate is high more than 3 times, the statistical procedures meaning is obvious.Obviously, the made product of this composting fermentation is brain disease patient's an outstanding protective foods, when obtaining high nutrition brain disorder can be obtained the effective treatment and prevent.
Embodiment 3: " five spirits are kept fit to drink " production technique
The functional beverage that this routine purpose provides a kind of high nutrition, keeps fit diseases prevention makes it drink at routine and realizes improving immunological competence subtlely, regulates viscera function, keeps fit the anti-aging effect of shape body.
Made glossy ganoderma, auricularia auriculajudae, the mushroom that high nutritive value is arranged for realizing that above purpose is got specially, the immunologic function of raising and regulating blood fat, the glossy ganoderma of blood sugar effect, Poria cocos are arranged, the dictyophora phalloidea that easypro liver, function of invigorating spleen are arranged, totally five bacterial classifications, by 0.5 (glossy ganoderma): 1 (auricularia auriculajudae): 1 (dictyophora phalloidea): the bacterial classification mixed of 2 (Poria cocos) is inoculated into shaking bottle and is obtained a mixed strains.The processing condition of shaking table are 26 ℃-32 ℃ of temperature, and mixing parametric: 200-300rpm, incubation time are 50-75 hour.Technology can be obtained three grades of liquid spawns of amplifying and turning out with reference to embodiment 2 given technical process later on, inserting in the substratum in fermentor tank fermentation again obtains to be rich in and mixes mycelial stoste, stoste is added batching after the concentration after filtration, can obtain solid kind food as the flours vehicle, adding toning, seasoning class batching can be made into oral liquid or contain the beverage of suspensoid.Carbon-nitrogen ratio with reference to the substratum proportioning is 30: 1, is pH value 4.5-6.5 with reference to processing condition, temperature 28-32 ℃, and pressure 0.07MPa, rotating speed 200-300rpm, fermentation time 40-60 hour.This mixed bacterium silk stock solution has detected following nutrition by analysis: polysaccharide 3-4%, amino nitrogen 0.03-0.05%, N.F,USP MANNITOL 2.5-3.5%, adenosine 0.01-0.029%, calcium 0.05-0.6%, phosphorus 0.04-0.05%, iron 0.04-0.06%, 16 kinds of amino acid kinds etc.As seen it is nutritious, be worth high, for lipopenicillinase, protect the liver, invigorating the spleen has tangible effect, that the experiment volunteer who takes for a long time all feels is energetic, spirit is vigorous, the good appetite.The nutritive value of this protective foods is described and improve immunity function very remarkable.
Embodiment 4: " four tuckahoe oral liquids " production technique
This routine purpose provides the high nourishing beverage that the cardiovascular rehabilitation and health care of patient of a kind of confession is used, and the prevention to liver-kidney diseases simultaneously has active effect.Choose the very high precious bacterial classification Cordyceps sinensis of a kind of pharmaceutical use for this this example, this medicinal material is famous and precious, cultivates complexity, and the medicine source is in great shortage.Can utilize industrial production to obtain being rich in the mycelium of effective ingredient easily by zymotechnique, realize that effective compatibility uses.This example is also chosen the other three kinds of bacterial classifications of Poria cocos, umbellate pore furgus and honey mushroom.Because breeding strain and other three kinds of bacterial classifications of Cordyceps sinensis have than big-difference, so adopt another kind of mixed fermentation form in this technology.Being divided into two technical process at the liquid spawn cultivating process finishes.Cordyceps sinensis inserted separately shake bottle, be equipped with special culture media, finish shaking table and three grades of seeding tanks and amplify and produce liquid spawn.(Poria cocos: umbellate pore furgus: honey mushroom) ratio was inserted and is shaken bottle and finish three grades of seeding tanks and cultivate to amplify and produce the mixing liquid bacterial classification with 2: 1: 2 with other three kinds of bacterial classifications.The processing condition of shaking table are basic identical, are: 25 ℃ of culture temperature, shaking table parameter 180rpm, incubation time 3-4 days.The liquid spawn and the mixing liquid bacterial classification of Cordyceps sinensis are finished mixed fermentation in fermentor tank process.Concrete method is: mixed strains is inserted in the fermentor tank ferment earlier, this moment, the volume of substratum was 1/2 jar, at definite processing condition bottom fermentation after 1 day half to two day, the special culture media that adds Cordyceps sinensis again, insert Cordyceps sinensis liquid spawn and the former mixed fermentation simultaneously and finished fermentation in 1 day half to two day, obtain to such an extent that be rich in four kinds of mycelial stostes.The substratum of Cordyceps sinensis is based on yeast powder, glucose, sucrose, and is equipped with bean powder, Semen Maydis powder, batching carbon-nitrogen ratio and is: (10-16): 1.Then three's substratum is based on Semen Maydis powder, wheat bran, glucose, a spot of inorganic salt and yeast powder in addition, and matching principle is that carbon-nitrogen ratio is (20-30): 1.Processing condition in the fermentor tank are: temperature 20-25 ℃, and mixing parametric 150-180rpm, pH value 5-6.5, air flow 1: 0.5, pressure 0.05MPa.In the Cordyceps mycelium process of growth back three kinds of mycelia there is certain restraining effect, so take above corresponding mixed fermentation technology.Utilize the stoste that is generated to filter, concentrate the back and blend four tuckahoe oral liquids that promptly can make high pharmaceutical use with seasonings.By a large amount of classification experiments, prove that it is good assisting therapy effect to cardiovascular disorder, hepatitis, renal tubal dysfunction or cerebral infarction sequela, be a kind of fabulous health care medicine of such patient.Also be a kind of fabulous nutritious prod, for dietotherapy provides a fine kind.
In sum, by the mixed fermentation technology that the present invention started, can ideally solve food medicine batrachians fungi organically combines, the problem of having complementary advantages, when making nutrition perfect, high-quality, realized the complementary and reinforcement of pharmaceutical use, particularly it utilizes zymotechnique to realize extracting in the suitability for industrialized production of fungi effective ingredient, thereby fungi eats and pharmaceutical use provides a brand-new technical scheme in order to bring into play.
Claims (10)
1, mixed fermentation technology for health-care nutritive food, comprise cultivation and the amplification of strain improvement, liquid spawn in its technical process, in fermentor tank, produce and be rich in mycelial stoste, obtain the finished product after concentrating again after filtration,, add batching, sterilization, it is characterized in that selected bacterial classification is to have edible or pharmaceutical use more than two kinds, or the fungi strain combined inoculation fermentation edible, that pharmaceutical use has concurrently, obtain the finished product through above-mentioned technical process.
2, according to the said mixed fermentation technology of claim 1, it is characterized in that selected bacterial classification is auricularia auriculajudae, mushroom and three bacterial classifications of Poria cocos, 1: 1: 2 ratio of inoculum size is seeded in same shaking and cultivates into mixed strains in the bottle routinely, the CONTROL PROCESS condition is: 25 ℃-30 ℃ of temperature, mixing parametric 150-180rpm, time 86-96 hour, to cultivate again successful mixed strains with 10% inoculum size in seeding tank three grades cultivate to amplify and to make liquid spawn, again liquid spawn is introduced substratum and in fermentor tank, ferment to obtain and be rich in the mycelial stoste of mixing.
3, according to the said mixed fermentation technology of claim 2, it is characterized in that substratum is made up of Semen Maydis powder, flour, bean powder, a little sugar, yeast powder and inorganic salt, by N: C=(20-40): 1 principle proportioning and obtaining, technological condition for fermentation is: 25 ℃-30 ℃ of temperature, pressure 0.05-0.08MPa, air flow 1: 0.6-1: 1, pH value 4.5-6.5, stirring velocity 150-200rpm, fermentation time 40-60 hour.
4, according to claim 1 or 2 said mixed fermentation technologies, it is characterized in that add batching and comprise and make the essential flour of biscuit shape food, fruit juice, milk powder, salt compounded of iodine, egg, vegetables oil, essence, sweet Stevia and an amount of saleratus power.
5, according to the said mixed fermentation technology of claim 1, it is characterized in that selected bacterial classification is black fungus and honey mushroom, 1: 2 mixed of inoculum size is inoculated in to shake and cultivates into mixed strains in the bottle routinely, culture condition is 30 ℃-35 ℃ of temperature, shaking table rotational parameters 200-300rpm, time 40-80 hour, again above mixed strains is gone into seeding tank by 10% amount inoculation and carry out three grades of liquid spawns of amplifying the system mixed bacterium, again liquid spawn is introduced special culture media and in fermentor tank, ferment and obtain to be rich in the mycelial stoste of mixing.
6, according to the said mixed fermentation technology of claim 5, it is characterized in that in this technology that said substratum is to add with 7.5% chrysanthemum to join 11.5% Vegetable Sponge of Luffa to decoct the back liquid that obtained be base fluid, press 30: 1 carbon-nitrogen ratio principle again, add and join Semen Maydis powder, bean powder, sucrose, wheat bran, yeast and inorganic salt and make, the fermentor tank processing condition are: 30 ℃-35 ℃ of temperature, pressure 0.06-0.08MPa, air flow is 1: 1, mixing parametric 200-300rpm, pH value 6.7-7.0, fermentation time 35-45 hour.
7,, it is characterized in that adding and comprise in the batching and make the required fruit juice of oral liquid, sucrose, seasonings and an amount of sanitas according to claim 1 or 5 said mixed fermentation technologies.
8, according to the said mixed fermentation technology of claim 1, it is characterized in that selected bacterial classification is a glossy ganoderma, auricularia auriculajudae, mushroom, dictyophora phalloidea and Poria cocos be inoculum size 0.5: 1: 1 routinely: 1: 2 mixed is inoculated in one and shakes to cultivate in the bottle and obtain mixed strains, the shaking table processing condition are: 26 ℃-32 ℃ of temperature, mixing parametric: 200-300rpm, incubation time 50-75 hour, and obtain three grades of amplification culture with reference to claim 2 method and become liquid spawn, inserting substratum again ferments to obtain to be rich in fermentor tank and mixes mycelial stoste, stoste is concentrated the adding seasonings can obtain the oral liquid series products, or adding flours vehicle can obtain Biscuits food.
9, according to the said mixed fermentation technology of claim 1, it is characterized in that selected bacterial classification is a Cordyceps sinensis, Poria cocos, umbellate pore furgus and armillaria mellea, wherein Cordyceps sinensis is implanted separately and is shaken bottle, the back is inoculated into shaking bottle with 2: 1: 2 mixed for three kinds, respectively at 25 ℃, 180rpm cultivated 3-4 days down, draw Cordyceps sinensis and three bacterium mixed strainss, both are obtained two kinds of liquid spawns through three grades of seed tank culture respectively, earlier mixed strains is implanted in the inherent fermentor tank of substratum fermentation then after 2 days, in jar, add the special culture media of joining Cordyceps sinensis, and then the liquid spawn and the former mixed fermentation of implantation Cordyceps sinensis made the final five kinds of mycelial stostes that are rich in two days.
10, according to the said mixed fermentation technology of claim 9, the special culture media that it is characterized in that Cordyceps sinensis is based on yeast powder, glucose, sucrose, suitably be furnished with bean powder, corn by C: N10-16: 1 proportionality principle is mixed with, the substratum of mixed bacterium with corn, wheat bran, glucose serve as the main small amounts of inorganic salt, yeast of adding by C: N=(20-30): 1 principle proportioning is produced, other processing condition in fermentor tank are: temperature 20-25 ℃, mixing parametric 150-180rpm, pH value 5-6.5, air flow 1: 0.5, pressure 0.05MPa.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103315362A (en) * | 2013-07-16 | 2013-09-25 | 河北大学 | Poria cocos solid state fermentation functional beverage and its preparation method |
| CN103393095A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Processing method of compound hypha powder |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN104970354A (en) * | 2014-04-04 | 2015-10-14 | 浙江省医学科学院 | Functional food for reducing blood fat and blood pressure and two-step fermentation preparation method of functional food |
| CN106306955A (en) * | 2016-08-24 | 2017-01-11 | 开平市水口镇卡摩商行 | Preparing method of armillaria mellea drinks |
| CN108260808A (en) * | 2018-01-31 | 2018-07-10 | 万宁万维诺丽生物科技有限公司 | A kind of beautiful ferment of promise and preparation method thereof |
| CN108967049A (en) * | 2018-07-05 | 2018-12-11 | 贵州省农作物品种资源研究所 | A kind of Dictyophora rubrovalvata liquid spawn quick-breeding method |
-
1998
- 1998-06-09 CN CN 98102071 patent/CN1204692A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103315362A (en) * | 2013-07-16 | 2013-09-25 | 河北大学 | Poria cocos solid state fermentation functional beverage and its preparation method |
| CN103315362B (en) * | 2013-07-16 | 2014-11-05 | 河北大学 | Poria cocos solid state fermentation functional beverage and its preparation method |
| CN103393095A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Processing method of compound hypha powder |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN103393094B (en) * | 2013-07-19 | 2014-07-09 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN104970354A (en) * | 2014-04-04 | 2015-10-14 | 浙江省医学科学院 | Functional food for reducing blood fat and blood pressure and two-step fermentation preparation method of functional food |
| CN106306955A (en) * | 2016-08-24 | 2017-01-11 | 开平市水口镇卡摩商行 | Preparing method of armillaria mellea drinks |
| CN108260808A (en) * | 2018-01-31 | 2018-07-10 | 万宁万维诺丽生物科技有限公司 | A kind of beautiful ferment of promise and preparation method thereof |
| CN108967049A (en) * | 2018-07-05 | 2018-12-11 | 贵州省农作物品种资源研究所 | A kind of Dictyophora rubrovalvata liquid spawn quick-breeding method |
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