CN1086115C - Preparation process and product of nutritive glossy ganoderma liquid - Google Patents
Preparation process and product of nutritive glossy ganoderma liquid Download PDFInfo
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- CN1086115C CN1086115C CN97121602A CN97121602A CN1086115C CN 1086115 C CN1086115 C CN 1086115C CN 97121602 A CN97121602 A CN 97121602A CN 97121602 A CN97121602 A CN 97121602A CN 1086115 C CN1086115 C CN 1086115C
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Abstract
The present invention relates to a preparation method and a product of nutritive glossy ganoderma liquid. The preparation method of the present invention mainly comprises the following processes: inclined plane strain culture, shake flask strain culture and liquid fermentation in a shake flask. Particularly, nutritive liquid obtained by decocting various famous and precious traditional Chinese medicines is added into a culture medium for liquid fermentation so that the nutritive glossy ganoderma liquid which is the product prepared in the method is rich in hydrolysed protein, seventeen amino acids, glossy ganoderma polysaccharide, nucleotide, thirteen biologic enzymes, fifteen mineral elements, alkaloid, mannitol and multiplex vitamins, and therefore, the nutritive glossy ganoderma liquid performs the functions of easy human body digestive absorption, human body ageing delay, endocrine function promotion, organism biologic enzyme activation, and immune system enhancement, and the nutritive glossy ganoderma liquid has the efficacy of various in vivo impurity removement, metabolic detoxification promotion, various difficult miscellaneous disease prevention and treatment, etc.
Description
The invention belongs to a kind of nourishing beverage, particularly relate to preparation method of a kind of glossy ganoderma nutrient solution and products thereof.
Along with the fast development of modern Human physiology, medical science and trophology, the Ji Li of human senility, pathology progressively obtains disclosing: the multiple disease of human body and aging often be since in the human organ function overbalance, immunologic function decline, body in biological enzyme and endocrine disturbance, the body potential of hydrogen (pH value) change, in the human body body in long-term contamination and the body rubbish can not get removing cause.How can make oneself to delay senility, the opposing various diseases, good health and a long life are things that each modern is concerned about most.
Glossy ganoderma is considered as the highest tonic herb by people since ancient times, it can preserve youthful looks and foster the spirit of nobility, strong smart, enrich blood, promote longevity, control all kinds of diseases and ailments, particularly magical effect is often taken place in many difficult and complicated cases.Because of glossy ganoderma system grows on the exhausted precipice, remote mountains, quantity is few, be difficult for taking, Gu be considered as rare treasure by common people, seldom at a draft all the life.Yet glossy ganoderma is notorious to the special curative effect of human body: to the effect of respiratory system, to the effect of cardiovascular system, to the effect of vascular hypertension, to the curative effect of hepatitis, to the effect of Digestive tract, to neural curative effect, to the curative effect of leukopenia, to Immune Effects and antitumor action, and various bacteriums and other illness of causing of virus are all had certain curative effect.
The purpose of this invention is to provide a kind of preparation method who obtains glossy ganoderma nutrient solution with multiple precious Chinese medicine as the natural wild Ganoderma of fermention medium liquid fermenting.
Another object of the present invention provides glossy ganoderma nutrient solution and the series product thereof that above-mentioned preparation method makes.
The preparation method of glossy ganoderma nutrient solution of the present invention comprises that slant strains cultivates, shakes a bottle spawn culture, shaking culture and shake flask fermentation and cultivate.
Described slant strains culturing process comprises that arranging inclined-plane, cooling back inoculation culture to mycelia behind culture medium preparation, packing test tube, the high pressure sterilization covers with that to put the refrigerator place behind the pipe standby.
Contain Ma Linggu, sucrose, potassium primary phosphate, lime carbonate, sal epsom, agar and water in the described substratum, wherein the parts by weight proportioning of each composition is:
Potato 20-30 part sucrose 1-3 part
KH
2PO
40.1-0.3 part CaCO
30.1-0.3 part
MgSO
40.05-0.2 part agar 2-4 part
100 parts in water
The row inclined-plane is that PH with substratum is adjusted to 6 behind the described high pressure sterilization, arranges the inclined-plane again after keeping 20-30 minute under 1-2 kilogram pressure, 120～130 ℃ of temperature, after the cooling, keeps 28-32 ℃ of constant temperature standby; Described postvaccinal culturing process is to cultivate 10～15 days at 26 ℃～28 ℃ thermostat containers.
The described bottle spawn culture process of shaking of the present invention comprises after bottle, sterilization, access inclined-plane kind are shaken in culture medium preparation, packing and places shaking table.
Described shaking in bottle culture of strains base contained wheat skin, sucrose and water, and three's parts by weight proportioning is:
Wheat skin 1-3 part, 100 parts in sucrose 1-3 part water
During preparation, earlier the wheat skin is boiled the back and kept 25-35 minute, cross leaching filtrate,, transfer PH to 6.5, boil the back packing with sucrose with slow fire; Described sterilization is that substratum was kept 30～40 minutes under 120～130 ℃, 1-2kg pressure.
Described shaking culture of the present invention is that the postvaccinal bottle that shakes is placed on the shaking table, and shaking culture is 96～120 hours under the condition of 26 ℃～28 ℃ temperature, per minute vibration 110～130 times.
Described shake flask fermentation culturing process of the present invention comprises that culture medium preparation, packing shake after bottle, autoclaving, the cooling inoculation, shaking culture after 5～7 days, get bacterium liquid, through 90～100 ℃, kept 25～35 minutes, thickening filtration is got filtrate can obtain glossy ganoderma nutrient solution.
The raw material of the substratum that described shake flask fermentation is used contains bean powder, peanut powder, Semen Maydis powder, sucrose, potassium primary phosphate, lime carbonate, water and Chinese medicine Radix Codonopsis, the Radix Astragali, Radix Angelicae Sinensis, longan, red date, matrimony vine, the bighead atractylodes rhizome and the sheep leaves of pulse plants, and wherein the parts by weight proportioning of various compositions is:
0.5～1.5 part of Semen Maydis powder 2-4 of bean powder 1-3 part peanut powder part
Sucrose 1-3 part KH
2PO
40.05～0.15 part of CaCO
30.05～0.15 part
100 parts in water
0.05～0.15 part of 0.1～0.3 part of Radix Angelicae Sinensis of 0.1～0.3 part of Radix Astragali of Radix Codonopsis
0.1～0.3 part of 0.1～0.3 portion of matrimony vine of 0.1～0.3 portion of red date of longan
0.05～0.15 part of 0.05～0.15 portion of sheep of bighead atractylodes rhizome leaves of pulse plants
During preparation, bean powder, peanut powder and Semen Maydis powder are boiled back slow fire respectively kept 40～50 minutes, get after filtrate merges, add KH
2PO
4, CaCO
3And sucrose, after stirring, transfer PH to 6; Chinese medicine is boiled after crushed, and slow fire boiling 1～1.5 hour is got filtrate and is mixed with above-mentioned filtrate, mixes well the back packing and shakes bottle, keeps 30-40 minute under 1～2 kilogram of pressure, 120～130 ℃ of temperature; The temperature of described postvaccinal shaking culture is 22～28 ℃, per minute vibration 110～130 times.
It is to control with 3～7% rare lactic acid or 3～7% sodium hydroxide that PH described in the present invention regulates.
Can also contain V in the described shake flask fermentation substratum
E, V
B1, V
C, ratio is to add V in per 100 milliliters of substratum
E, V
B1, V
CEach 0.1 milligram.
Can also be included among the preparation method of the present invention and add the high-quality Sorghum vulgare Pers. Wine in the lucidum bacteria liquid in proportion, stir the static immersion in back 12-18 days,, fill a wine cup for and thalline separates, get clear filtrate and get final product through centrifuging.
Can also comprise among the preparation method of glossy ganoderma nutrient solution of the present invention qualified glossy ganoderma thalline through 50～70 ℃ of hot-air seasonings, again through 4～8 ℃ of cryodryings, get final product after pulverizing.
The preparation method of glossy ganoderma nutrient solution of the present invention is scientific and reasonable, because of having adopted liquid fermenting natural glossy ganoderma bacterium, bacterium liquid after the fermentation has produced a large amount of nutrient substances after testing, particularly the content of ten seed amino acids is high, and the colloid albumen with delaying human body caducity effect is abundanter than Ganoderma sporophore especially, particularly fermented liquid and mycelium pellet also secrete a large amount of biological enzymes and biologically active substance, not only make all nutrients and the effective ingredient of glossy ganoderma all be dissolved in the fermented liquid, and its quality is improved greatly, and easilier directly absorbed by stomach.
Among the preparation method of glossy ganoderma nutrient solution of the present invention, owing to adopted the fermention medium of multiple precious Chinese medicine as Ganderma lucidum, and make it on the basis of all nutritions that contain glossy ganoderma, also have nutrition more comprehensively, abundanter, the tonifying Qi effect stronger, that curative effect is higher of enriching blood, and there is not irritating taste of traditional Chinese medicine, better than the color of the ganoderma lucidum prod of processing with Ganoderma sporophore.
Following table is the analytical test result of this glossy ganoderma nutrient solution.
Test result: soluble proteins (in crude protein): 0.69g/100ml soluble polysaccharide (with glucose meter): 2.6g/100ml
| Title | Unit | Content | Title | Unit | Content |
| Aspartic acid | mg/100ml | 61.0 | Tyrosine | mg/100ml | 18.9 |
| L-glutamic acid | mg/100ml | 68.9 | Xie Ansuan | mg/100ml | 10.9 |
| Serine | mg/100ml | 11.6 | Methionine(Met) | mg/100ml | 6.90 |
| Glycine | mg/100ml | 22.6 | Gelucystine | mg/100ml | 5.58 |
| Histidine | mg/100ml | 4.41 | Isoleucine | mg/100ml | 8.93 |
| Arginine | mg/100ml | 12.4 | Leucine | mg/100ml | 18.9 |
| Threonine | mg/100ml | 9.27 | Phenylalanine | mg/100ml | 28.3 |
| L-Ala | mg/100ml | 21.5 | Methionin | mg/100ml | 11.1 |
| Proline(Pro) | mg/100ml | 7.33 | ---- |
Testing method: with reference to GB 5009-85 and Q/FCB 012-92 (ICP method)
Test result: zinc (Zn): 7.84mg/100ml calcium (Ca): 7.91mg/100ml
Iron (Fe): 1.05mg/100ml phosphorus (P): 91.0mg/100ml
Adopt the preparation method of glossy ganoderma nutrient solution of the present invention can obtain glossy ganoderma nutrient solution.This product can be eliminated impurity in the blood, make each organ cell replenish nutrient and oxygen, make that cell is regenerated rapidly, pre-anti-aging, eliminate speckle, acne, comedo, minimizing wrinkle, strengthen skin elasticity, keep human body natural U.S. and youthful vigor, be any makeup and medicine can not than.Particularly this product facts have proved the clinical efficacy of human body, and this product reaches 70～97.6% to the total effective rate of chronic tracheitis, pneumonia, pulmonary tuberculosis, allergic rhinitis and asthma; Treatment coronary heart disease, stenocardia, hyperlipidemia and anaemia are had remarkable effect, and especially the total efficiency to cardiovascular diseases reaches 79～93.6%; To vascular hypertension, reach 100% with efficient around the clothes with depressor; Total effective rate (1-3 month) to hepatitis reaches 97%, produce effects 70%; Stomach trouble, stomach ulcer, gastroptosis, acute enteritis and constipation all there is certain curative effect; Total effective rate to the neurasthenia illness reaches 100%; Total effective rate to leukopenia reaches more than 81.7%; To treating rheumatic arthritis, ephritis, hyperthyroidism, cerebral concussion sequela, and the physique after the radiotherapy, chemotherapy recovers all have certain effect, particularly this product can also strengthen the immune function of human body effect, improves antibiotic, antiviral, anti-tumor capacity.
Adopt preparation method of the present invention also can obtain Lucid Ganoderma wine.It is powerful that this Lucid Ganoderma wine not only has the institute of glossy ganoderma nutrient solution, but also can be promoting blood circulation and removing blood stasis, when enjoying good wine and dainty dishes, accelerate to absorb how better nutrient.
Adopt preparation method of the present invention can also obtain Ganoderma tea.This Ganoderma tea is because of being directly to adopt Ganoderma mycelium processing get, thus have fully glossy ganoderma nutrient solution powerful.
Adopt preparation method of the present invention also can further obtain series product such as Ganoderma beverage, lucid ganoderma beautifying dew and glossy ganoderma fair complexion cream.
Further specify the present invention below in conjunction with embodiment.
Prepare a kind of glossy ganoderma nutrient solution, process is as follows:
(1) selects 25 parts of potatos, 2 parts of sucrose, KH for use
2PO
40.2 part, CaCO
30.2 part, MgSO
40.1 part, the water of 3 parts in agar and 100 parts is mixed with the female substratum of planting in inclined-plane, divides to install in the test tube, keeps the sterilization that carries out disinfection in 25 minutes under 1.5 kilograms of pressure, 126 ℃ of temperature, the row inclined-plane, placement 30 ℃ of thermostat containers in cooling back are standby; By strain domestication, screening are purified, select to be fit to liquid fermenting, resist the assorted strong bacterial strain of bacterium ability to inoculate, be placed on after the inoculation in 28 ℃ of thermostat containers and cultivated 10 days, after mycelia was covered with pipe, it was stand-by to put refrigerator.
(2) select the water of 2 parts of 2 parts of wheat skins, sucrose and 100 parts for use, after earlier the wheat skin being boiled, slow fire kept 30 minutes, crossed leaching filtrate, with sucrose, after mixing well, regulate PH to 6.5, branch installs to triangle and shakes in the bottle, keeps 30 minutes under 1.5 kilograms pressure, 126 ℃ of temperature, after cooling, the inclined-plane kind is moved receive triangle shake the bottle liquid in; Postvaccinal triangle is shaken bottle be placed on the shaking table, constant-temperature shaking culture, temperature are controlled at 26 ℃, and oscillation number is 120 times/minute, cultivates after 100 hours, and it is bottle stand-by as fermented bacterium to select bacterium liquid clarification, bacterium ball growth triangles many, that do not have the bacterium of mixing to shake; During shaking culture, cultivate the microscopy of taking a sample after 24 hours, observe whether microbiological contamination, and the pH value of mycelial growth situation and test bacterium liquid.
(3) select 2 parts of bean powderes, 1 part of peanut powder, 3 portions of Semen Maydis powder, 2 portions of sucrose, 0.1 part of KH for use
2PO
4, 0.1CaCO
3And 100 parts water, earlier bean powder, peanut powder, Semen Maydis powder are boiled the back respectively and kept 45 minutes with slow fire, get filtrate merging, add KH
2PO
4, CaCO
3And sucrose mixes stand-by; Select 0.1 part of 0.2 part of Radix Codonopsis, 0.2 part of the Radix Astragali, 0.1 part of Radix Angelicae Sinensis, 0.2 part of longan, 0.2 part of red date, 0.2 part of matrimony vine, 0.1 part of the bighead atractylodes rhizome and the sheep leaves of pulse plants again for use, boil together after crushed, get filtrate with slow fire boiling after 1 hour, mix with the above-mentioned filtrate that contains compositions such as bean powder, transfer pH value to 6, branch installs to triangle and shakes in the bottle, keeps 30 minutes sterilizations under 1.5 kilograms of pressure, 126 ℃ of temperature, the inoculation of cooling back; Postvaccinal triangle is shaken a bottle placement shaking table ferment, promptly cultivated 150 hours down at 26 ℃.The microscopy of taking a sample after cultivating 24 hours is observed mycelium germination growing state and bacterium liquid color, bacterium ball size, bacterial concentration, and microscopy is once after 12 hours in every cultivation.
(4) observe through microscopy, qualified lucidum liquid should pollution-free, no any assorted bacterium, the clarification of bacterium liquid, thickness, and taste delicate fragrance, no tart flavour, free from extraneous odour, bacterium ball are golden yellow, and is translucent, is covered with bacterium liquid, and centrifugal by whizzer, bacterium ball humidity is higher than more than 50%.
Get the lucidum bacteria liquid that is up to the standards 100 ℃, kept 30 minutes, thickening filtration is got the filtrate bottling.Carry out vacuum exhaust, capping after the bottling, keep the sterilization that carries out disinfection in 20 minutes down, be purpose product-glossy ganoderma nutrient solution at 120 ℃.
Claims (8)
1, a kind of preparation method of glossy ganoderma nutrient solution is characterized in that comprising the slant strains cultivation, shakes bottle spawn culture and shake flask fermentation cultivation; Described shake flask fermentation culturing process is got bacterium liquid after comprising that inoculation after bottle, autoclaving, the cooling, shaking culture 5-7 days are shaken in culture medium preparation, packing, through 90-100 ℃, kept 25-35 minute, thickening filtration is got liquid can obtain glossy ganoderma nutrient solution;
The raw material of the substratum that described shake flask fermentation is used contains bean powder, peanut powder, Semen Maydis powder, sucrose, potassium primary phosphate, lime carbonate, water and Chinese medicine Radix Codonopsis, the Radix Astragali, Radix Angelicae Sinensis, longan, red date, matrimony vine, the bighead atractylodes rhizome and the sheep leaves of pulse plants, and wherein the parts by weight proportioning of various compositions is:
Bean powder 1-3 part peanut powder 0.5-1.5 part Semen Maydis powder 2-4 part
Sucrose 1-3 part KH
2PO
40.05-0.15 part CaCO
30.05-0.15 part
Radix Codonopsis 0.1-0.3 part Radix Astragali 0.1-0.3 part Radix Angelicae Sinensis 0.05-0.15 part
Longan 0.1-0.3 part red date 0.1-0.3 part matrimony vine 0.1-0.3 part
100 parts in bighead atractylodes rhizome 0.05-0.15 part sheep leaves of pulse plants 0.05-0.15 part water
During preparation, bean powder, peanut powder and Semen Maydis powder are boiled back slow fire respectively kept 40-50 minute, get after filtrate merges, add KH
2PO
4, CaCO
3And sucrose, after stirring, transfer PH to 6; Chinese medicine is boiled after crushed, slow fire boiling 1-1.5 hour, get filtrate and mix with above-mentioned filtrate, mix well the back packing and shake bottle, under 1-2 kilogram pressure, 120-130 ℃ temperature, kept 30-40 minute; The temperature of described postvaccinal shaking culture is 22-28 ℃, per minute vibration 110-130 time.
2, preparation method as claimed in claim 1 is characterized in that described slant strains culturing process comprises that arranging inclined-plane, cooling back inoculation culture to mycelia behind culture medium preparation, packing test tube, the high pressure sterilization covers with that to put the refrigerator place behind the pipe standby;
Contain potato, sucrose, potassium primary phosphate, lime carbonate, sal epsom, agar and water in the described substratum, wherein the parts by weight proportioning of each composition is:
Potato 20-30 part sucrose 1-3 part
KH
2PO
40.1-0.3 part CaCO
30.1-0.3 part
MgSO
40.05-0.2 part agar 2-4 part
100 parts in water
The row inclined-plane is that PH with substratum is adjusted to 6 behind the described high pressure sterilization, arranges the inclined-plane again after keeping 20-30 minute under 1-2 kilogram pressure, the 120-130 ℃ temperature, after the cooling, keeps 28-32 ℃ of constant temperature standby; Described postvaccinal culturing process is to cultivate 10-15 days at 26 ℃ of-28 ℃ of thermostat containers.
3, preparation method as claimed in claim 1 is characterized in that the described bottle spawn culture process of shaking comprises after bottle, sterilization, access inclined-plane kind are shaken in culture medium preparation, packing and places shaking table; Under the condition of 26-28 ℃ of temperature, per minute vibration 110-130 time shaking culture 96-120 hour;
Described shaking in bottle culture of strains base contained wheat skin, sucrose and water, and three's parts by weight proportioning is:
Wheat skin 1-3 part, sucrose 1-3 part, 100 parts in water
During preparation, earlier the wheat skin is boiled the back and kept 25-35 minute, cross leaching filtrate,, transfer PH to 6.5, boil the back packing with sucrose with slow fire; Described sterilization is that substratum was kept 30-40 minute under 120-130 ℃, 1-2kg pressure.
4, a kind of preparation method of Lucid Ganoderma wine is characterized in that adding Sorghum vulgare Pers. Wine in proportion in the lucidum bacteria liquid that adopts one of claim 1-3 preparation method to make, and stirs the static immersion in back 12-18 days, through centrifuging, fills a wine cup for and thalline separates, and gets clear filtrate and gets final product.
5, a kind of preparation method of Ganoderma tea is characterized in that adopting glossy ganoderma thalline that the preparation method of one of claim 1-3 makes through 50-70 ℃ of hot-air seasoning, again through 4-8 ℃ of cryodrying, get final product after pulverizing.
6, a kind of glossy ganoderma nutrient solution is characterized in that the prepared product as the preparation method of one of claim 1-3.
7, a kind of Lucid Ganoderma wine is characterized in that the product that preparation method as claimed in claim 4 makes.
8, a kind of Ganoderma tea is characterized in that the product that makes by claim 5 preparation method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97121602A CN1086115C (en) | 1997-11-03 | 1997-11-03 | Preparation process and product of nutritive glossy ganoderma liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97121602A CN1086115C (en) | 1997-11-03 | 1997-11-03 | Preparation process and product of nutritive glossy ganoderma liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1181901A CN1181901A (en) | 1998-05-20 |
| CN1086115C true CN1086115C (en) | 2002-06-12 |
Family
ID=5176316
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97121602A Expired - Fee Related CN1086115C (en) | 1997-11-03 | 1997-11-03 | Preparation process and product of nutritive glossy ganoderma liquid |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103141891B (en) * | 2013-02-28 | 2018-01-19 | 江南大学 | A kind of preparation method of antrodia camphorata liver protection functional beverage |
| CN104839382B (en) * | 2014-06-20 | 2018-01-16 | 三峡大学 | A kind of ganoderma lucidum Chinese flowering crabapple tea and preparation technology and its application |
| CN104823716A (en) * | 2015-05-08 | 2015-08-12 | 郭亚涛 | Culture and preparing method of fungus symbiotic hypha powder |
| CN105176679A (en) * | 2015-08-20 | 2015-12-23 | 嘉兴景程生物科技有限公司 | Cigarette essence containing lucid ganoderma fermentation broth and preparation method thereof |
| CN107541440A (en) * | 2017-10-18 | 2018-01-05 | 湖州新驰医药科技有限公司 | A kind of preparation method of edible mushroom wine |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066882A (en) * | 1992-06-03 | 1992-12-09 | 党文平 | The manufacture craft of scorpion wine |
| CN1105062A (en) * | 1993-09-29 | 1995-07-12 | 德阳市灵芝厂 | Recipe of Lucid Ganoderma wine and its brewing technology |
| CN1105208A (en) * | 1994-09-28 | 1995-07-19 | 湖南省益阳伊嘉生物工程公司 | Health beverage of glossy ganoderma series |
| CN1126036A (en) * | 1995-01-03 | 1996-07-10 | 汪士尚 | Compound bagged glossy ganoderma tea |
| CN1151847A (en) * | 1996-08-28 | 1997-06-18 | 海南保丽康生物科技食品有限责任公司 | Chinese glossy ganoderma health beverage |
-
1997
- 1997-11-03 CN CN97121602A patent/CN1086115C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066882A (en) * | 1992-06-03 | 1992-12-09 | 党文平 | The manufacture craft of scorpion wine |
| CN1105062A (en) * | 1993-09-29 | 1995-07-12 | 德阳市灵芝厂 | Recipe of Lucid Ganoderma wine and its brewing technology |
| CN1105208A (en) * | 1994-09-28 | 1995-07-19 | 湖南省益阳伊嘉生物工程公司 | Health beverage of glossy ganoderma series |
| CN1126036A (en) * | 1995-01-03 | 1996-07-10 | 汪士尚 | Compound bagged glossy ganoderma tea |
| CN1151847A (en) * | 1996-08-28 | 1997-06-18 | 海南保丽康生物科技食品有限责任公司 | Chinese glossy ganoderma health beverage |
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| Publication number | Publication date |
|---|---|
| CN1181901A (en) | 1998-05-20 |
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Effective date of registration: 20120202 Address after: Silver City Garden Gulou District of Nanjing city in Jiangsu province 210036 No. 49 room 701 Patentee after: Liu Zhongxue Address before: 362000, Quanzhou, Fujian Province Feng Feng street, 372, Quanzhou Province Tatsu fungus health care products factory Patentee before: Huang Shaoyun |
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