CN103141891B - A kind of preparation method of antrodia camphorata liver protection functional beverage - Google Patents
A kind of preparation method of antrodia camphorata liver protection functional beverage Download PDFInfo
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- CN103141891B CN103141891B CN201310063732.0A CN201310063732A CN103141891B CN 103141891 B CN103141891 B CN 103141891B CN 201310063732 A CN201310063732 A CN 201310063732A CN 103141891 B CN103141891 B CN 103141891B
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Abstract
The invention discloses a kind of antrodia drinks and preparation method thereof, the drink be by jujube it is cleaned, stoning, baking, crush, antrodia submerged fermentation is participated in the form of red date powder, the drink rich in the nutriments such as amino acid and the various active materials of antrodia is prepared.Contain triterpene, polysaccharide isoreactivity material and multiple nutritional components in this beverage, there is preferable liver protection curative effect.
Description
Technical field
The present invention relates to the fermentation of edible mushroom and processing, and in particular to the preparation method of antrodia camphorata liver protection functional beverage.
Background technology
Antrodia（Antrodia camphorata）, also known as Antrodia camphorata, cinnamomum kanahirai hay mushroom, red camphor tree wild rice etc. are to parasitize Taiwan mountain area
The basidiomycetes of the rotten heartwood inwall of cinnamomum kanehirai between 450 ~ 2000m of height above sea level, it is a kind of distinctive medicinal fungi in Taiwan.As spy
Some species, in history antrodia by locals as sobering up and antifatigue traditional herbal medicine, be also used for treat liver diseases, food
Thing and drug-induced poisoning symptom and diarrhoea, stomachache, hypertension, skin are itched with tumor disease etc..Since nineteen ninety, camphor tree
After sesame is reported as a kind of new species, so far, isolated 70 multiple compounds from antrodia fructification, are wrapped
Derivative, ubiquinone class of polysaccharide, terpene, steroid, fumaric acid and butanedioic acid etc. are included, wherein majority is new compound.According to report
Road, polysaccharide, terpene and steroid compound are the main active substances of antrodia, have liver protection, anti-oxidant, anti-inflammatory, antitumor etc.
Curative effect.The derivative and general quinones substance of fumaric acid and butanedioic acid are also because its good drug effect is by extensive concern.The wild number of antrodia
Amount is rare and growth is very slow, and mycelium fermentation, then the extraction functionality group from tunning are carried out using anamorphic strain
Point be realized is a most economical environmentally friendly antrodia development approach.
Jujube is rich in the compositions such as protein, fat, vitamin C, citrin, B family vitamin and phosphorus, calcium, iron, except
Enriching yin and nourishing kidney, tonifying middle-Jiao and Qi, foster the spirit of nobility outside the function of calming the nerves, moreover it is possible to improve body immunity, reduce serum cholesterol, protect liver
It is dirty, suppress cancer cell.
This patent, as one of fermentation medium main component, for antrodia submerged fermentation, is obtained rich in work using jujube
The antrodia camphorata liver protection functional beverage of property material and nutriment.
The content of the invention
It is an object of the invention to provide a kind of antrodia drinks rich in nutritional ingredient and active component, have preferable
Hepatoprotective effect.
The purpose of the present invention is realized by following scheme：
1. by the jujube of fresh mature by cleaning, being enucleated, 80-95 DEG C, baking 20-30 min, fructus corni deflation is baked to,
Slightly burnt odor taste.Red date powder is made after pulverizer crushes, it is standby.
2. Antrodia camphorata mycelium is forwarded in slant medium, 25 DEG C -28 DEG C, culture 15-20 d.Slant medium forms
For：Potato 20%, glucose 2%, agar 2%, pH are natural.
3. by the slant strains switching shaking flask in step 2, mode is that an inclined-plane connects three bottles, culture medium composition：Grape
Sugar 2%, dusty yeast 1%, magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, pH4-5；The mL of 500 mL triangular flasks liquid amount 100,
Rotary shaker culture, rotating speed 100r/min, 25 DEG C -28 DEG C of cultivation temperature, incubation time 10-15 d.
4. the antrodia filtering fermentation liquor in step 3 is obtained into antrodia spore, according to 1.5 × 105Individual/mL inoculum concentration access
In fermentation tank, speed of agitator 100-150r/min, 25 DEG C of -28 DEG C of cultures, throughput 1vvm, culture 2-4 d；The training of seeding tank
Support the essentially identical of base and shaking flask.
5. the antrodia seeding tank in step 4 is transferred into the fermentation equipped with fermentation medium according to 10%-20% inoculum concentration
In tank, 25 DEG C of-28 DEG C of cultures, rotating speed 100-150 r/min, throughput 1vvm, 6-8 d are cultivated, under antrodia biomass starts
It is reduced to fermentation termination；The culture medium of fermentation tank forms：Glucose 2%, dusty yeast 1%, red date powder 2%, magnesium sulfate 0.15%, phosphorus
Acid dihydride potassium 0.3%, pH4-5.
It is 6. the antrodia tunning homogenizer homogeneous obtained by step 5,1200-1500 bar homogeneous is complete to bacterial cell disruption
Entirely；Then through plate-frame filtering, canned, sealing, sterilize at 90 DEG C 15min, that is, the antrodia drinks of the present invention are made.
A kind of beneficial effect for the method for producing antrodia drinks of the present invention：
Products obtained therefrom of the present invention is rose liquid, has fruit fragrant, hay-scented and fragrance of a flower fragrance, taste sweet tea acid delicate fragrance,
Slightly bitter taste, returning for tasting are sweet；The present invention using jujube as a kind of raw material of antrodia submerged fermentation, by the nutriment in jujube with
Antrodia is preferably combined together.Antrodia has liver protection, anti-oxidant, anti-inflammatory, the pharmacological activity such as antitumor in itself, in addition jujube
Enriching yin and nourishing kidney, nourishing qi and blood, improve immunity and other effects so that the drug effect and nutritive value of this drink are higher；The inventive method
It can meet the market demand with prepare with scale antrodia drinks, be preferably the health service of the mankind.
Embodiment
Embodiment 1：Antrodia drinks are prepared according to following steps：
1. by the jujube of fresh mature by cleaning, being enucleated, 80 DEG C, 30 min of baking, fructus corni deflation is baked to, it is slightly burnt
Fragrance.Red date powder is made after pulverizer crushes, it is standby.
2. Antrodia camphorata mycelium is forwarded in slant medium, 28 DEG C, 20 d are cultivated.Slant medium forms：Ma Ling
Potato 20%, glucose 2%, agar 2%, pH are natural.
3. by the slant strains switching shaking flask in step 2, mode is that an inclined-plane connects three bottles, culture medium composition：Grape
Sugar 2%, dusty yeast 1%, magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, pH4.5；The mL of 500 mL triangular flasks liquid amount 100,
Rotary shaker culture, rotating speed are 100 r/min, 26 DEG C of cultivation temperature, the d of incubation time 12.
4. the antrodia filtering fermentation liquor in step 3 is obtained into antrodia spore, according to 1.5 × 105Individual/mL inoculum concentration access
In fermentation tank, the r/min of speed of agitator 100,26 DEG C of cultures, throughput 1vvm, 3 d are cultivated；The culture medium and shaking flask of seeding tank
It is essentially identical.
5. the antrodia seeding tank in step 4 is transferred in the fermentation tank equipped with fermentation medium according to 10% inoculum concentration,
26 DEG C of cultures, rotating speed 100 r/min, throughput 1vvm cultivate 8 d, and antrodia biomass is begun to decline as fermentation termination；Fermentation
The culture medium of tank forms：Glucose 2%, dusty yeast 1%, red date powder 2%, magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%,
pH4.5。
It is 6. the antrodia tunning homogenizer homogeneous obtained by step 5,1200 bar homogeneous are complete to bacterial cell disruption；So
By plate-frame filtering, canned, sealing, sterilize 15min at 90 DEG C, that is, the antrodia drinks of the present invention are made.
Embodiment 2：Antrodia drinks are prepared according to following steps：
1. by the jujube of fresh mature by cleaning, being enucleated, 90 DEG C, 20 min of baking, fructus corni deflation is baked to, it is slightly burnt
Fragrance.Red date powder is made after pulverizer crushes, it is standby.
2. Antrodia camphorata mycelium is forwarded in slant medium, 28 DEG C, 20 d are cultivated.Slant medium forms：Ma Ling
Potato 20%, glucose 2%, agar 2%, pH are natural.
3. by the slant strains switching shaking flask in step 2, mode is that an inclined-plane connects three bottles, culture medium composition：Grape
Sugar 2%, dusty yeast 1%, magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, pH4.5；The mL of 500 mL triangular flasks liquid amount 100,
Rotary shaker culture, rotating speed are 150 r/min, 28 DEG C of cultivation temperature, the d of incubation time 12.
4. the antrodia filtering fermentation liquor in step 3 is obtained into antrodia spore, accessed according to 10% inoculum concentration in fermentation tank,
The r/min of speed of agitator 100,26 DEG C of cultures, throughput 1vvm, cultivates 3 d；The culture medium of seeding tank and the basic phase of shaking flask
Together.
5. the antrodia seeding tank in step 4 is transferred in the fermentation tank equipped with fermentation medium according to 10% inoculum concentration,
26 DEG C of cultures, rotating speed 150 r/min, throughput 1vvm cultivate 6 d, and antrodia biomass is begun to decline as fermentation termination；Fermentation
The culture medium of tank forms：Glucose 2%, dusty yeast 1%, red date powder 2%, magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%,
pH4.5。
It is 6. the antrodia tunning homogenizer homogeneous obtained by step 5,1200 bar homogeneous are complete to bacterial cell disruption；So
By plate-frame filtering, canned, sealing, sterilize 15min at 90 DEG C, that is, the antrodia drinks of the present invention are made.
Embodiment 3：
Product is made in method mentioned in embodiment 2, and test data is as follows：
（1）Hydrolysis amino acid is analyzed
Hydrolysising amino acid content analysis result shows, rich amino acids in antrodia drinks, contains common 17 kinds
Amino acid.
The antrodia drinks hydrolysis amino acid analysis result of table 1
| Amino acid | Content（g/L） |
| Aspartic acid | 0.63 |
| Glutamic acid | 1.14 |
| Serine | 0.23 |
| Histidine | 0.11 |
| Glycine | 1.98 |
| Threonine | 0.19 |
| Arginine | 0.53 |
| Alanine | 0.65 |
| Tyrosine | 0.72 |
| Cysteine | 0.09 |
| Valine | 0.05 |
| Methionine | 0.26 |
| Phenylalanine | 0.14 |
| Isoleucine | 0.20 |
| Leucine | 0.28 |
| Lysine | 0.32 |
| Proline | 1.17 |
（2）Trace element analysis
Trace element analysis result shows that micro- species is enriched in antrodia drinks, containing K, Na, Ca, Mg,
The common elements such as Cu, Mn, Zn, Fe.The harmful elements such as Pb, As, Hg are not detected simultaneously.
The antrodia drinks trace element analysis result of table 2
| Element | Content（mg/L） |
| Zn | 5.05 |
| Fe | 0.36 |
| Cu | 0.05 |
| Mn | 0.02 |
| K | 910.80 |
| Na | 95.08 |
| Mg | 273.70 |
| Ca | 35.34 |
| Pb | Do not detect |
| As | Do not detect |
| Hg | Do not detect |
（3）Total triterpene class and polysaccharose substance assay
Total triterpene contentses are 30.37 mg/L, the g/L of Thick many candies content 10.5 in antrodia drinks.
（4）Other features：
Outward appearance：Liquid
Color and luster：Rose
Fragrance：Distinctive fruit perfume, hay-scented and the fragrance of a flower
Taste：It is sour-sweet, slightly bitter taste, return sweet
Embodiment 4：
Product is prepared with the method mentioned in embodiment 1, the zoopery for carrying out acute alcohol-induced hepatic injury tests it
Protective effect to liver.
Materials and methods：Drinks made from the embodiment of the present invention 2, dose is recommended to give 5 mL/kg by adult.Choosing
SPF male mice in kunming 30 is taken, is randomly divided into 3 groups：A（Blank control group）、B（Drinks group）、C（Negative control
Group）.Each group mouse enters experiment with fed standard chow, free water, adaptability after feeding 1 week.Each group mouse is prohibited before experiment
Food 8 hours, A, C group give distilled water, and B groups measure gavage this drinks by 1g/kg, and after the min of gavage 45, A groups, which are given, distills
Water, B, C group give 50% ethanol by 10 mL/kg.Gavage puts to death mouse after 12 hours, wins liver, and each group liver is determined after homogenate
MDA in tissue（MDA）, reduced glutathione（GSH）And serum triglyceride（TG）, respectively from TBA colorimetric methods,
DTNB colorimetric methods and GPO peroxidase method.As a result it see the table below 1.
Influence of the drinks of table 1 to MDA, GSH, TG of acute hepatic injury mice
| Group | MDA（nmol/mgprot） | GSH（mg/gprot） | TG（mg/dL） |
| A blank control groups | 1.12±0.33 | 27.40±2.77 | 124.23±15.22 |
| B drinks groups | 1.90±0.23 | 25.34±3.52 | 158.79±20.76 |
| C negative control groups | 2.70±0.10 | 20.11±2.98 | 204.33±43.65 |
As seen from the above table, drinks of the invention have significant protective effect to liver.
Claims (6)
1. a kind of antrodia camphorata liver protection functional beverage, it is characterised in that the beverage is to clean the jujube of maturation, be enucleated, drying, crushing
Afterwards, the submerged fermentation of antrodia is participated in, resulting antrodia drinks are rich in triterpene, polysaccharide liver-protecting activity composition, and a variety of dimensions
Raw element, amino acid nutrient composition, its preparation process include following six step：
(1) the culture raw material using the jujube of fresh mature as addition, cleans stoning, after toasted crushing, it is standby that red date powder is made；
Red date powder addition and other culture mediums composition are as follows in fermentation tank culture medium：Glucose 2%, dusty yeast 1%, red date powder 2%,
Magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, pH 4-5；
(2) inclined-plane culture is carried out to antrodia；
(3) cultured slant strains in previous step are utilized, switching is cultivated into shaking flask liquid seeds bottle, standby；
(4) previous step collected into cultured shaking flask liquid seeds be inoculated into seeding tank and cultivate, obtain fermentor liquid seed,
It is standby；
(5) gained red date powder and culture medium in the first step are poured into fermentation tank, after cooling subject to sterilization, gained in the 4th step sent out
In fermentation tank liquid seeds access fermentation tank, large-scale culture Antrodia camphorata mycelium；When mycelial biomass declines, as fermentation is whole
Point, tank can be put；
(6) to gained zymotic fluid homogenizer homogeneous in previous step, make mycelium broken uniform；By plate-frame filtering, then tank
Dress, seal, sterilizing, produce；
Wherein in (1) step, the preparation of red date powder：Then the jujube of fresh mature is toasted, kept through over cleaning, stoning
Temperature toasts 20-30min at 80-95 DEG C, is baked to fructus corni deflation, slightly burnt odor taste；Again powder is ground into through pulverizer.
2. according to the preparation method of the antrodia drinks described in claim 1, it is characterised in that (2) step of methods described
In, described slant medium composition is：Potato 20%, glucose 2%, agar 2%, pH are natural；25 DEG C -28 of cultivation temperature
DEG C, incubation time 15-20d.
3. according to the preparation method of the antrodia drinks described in claim 1, it is characterised in that (3) step of methods described
In, the seed culture in shaking flask, mode is that an inclined-plane connects three bottles, culture medium composition：Glucose 2%, dusty yeast
1%, magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, pH4-5；500mL triangular flask liquid amount 100mL, rotary shaker culture,
Rotating speed is 100r/min, 25 DEG C -28 DEG C of cultivation temperature, incubation time 10-15d.
4. according to the preparation method of the antrodia drinks described in claim 1, it is characterised in that (4) step of methods described
In, the culture is that seed is accessed in seeding tank according to 10% inoculum concentration, speed of agitator 100-150r/min, and fermentation temperature is
25 DEG C -28 DEG C, throughput 1v/vm, cultivate 2-4d；The culture medium of seeding tank is identical with Shake flask medium.
5. according to the preparation method of the antrodia drinks described in claim 1, it is characterised in that (5) step of methods described
In, the culture medium composition of the fermentation tank is：Glucose 2%, dusty yeast 1%, red date powder 2%, magnesium sulfate 0.15%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.3%, pH 4-5；Speed of agitator 100-150r/min, fermentation temperature are 25 DEG C -28 DEG C, throughput 1v/vm, culture
6-8d。
6. according to the preparation method of the antrodia drinks described in claim 2, it is characterised in that (6) step of methods described
In, the sterilizing methods are pasteurizations, and sterilize 15min at 90 DEG C.
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| CN103750455A (en) * | 2013-12-27 | 2014-04-30 | 彭聪 | Production method for antrodia camphorata beverage |
| CN103907727A (en) * | 2014-03-25 | 2014-07-09 | 李明聪 | Making method of antrodia camphorate tea and antrodia camphorate tea made by adopting making method |
| CN104186880A (en) * | 2014-07-11 | 2014-12-10 | 福建农林大学 | Antrodia mycelium flavor soft sweet and processing method thereof |
| CN105919063A (en) * | 2016-04-29 | 2016-09-07 | 上海理工大学 | Antrodia camphorata spore powder and use thereof |
| CN108175023A (en) * | 2018-03-01 | 2018-06-19 | 康师傅（昆明）饮品有限公司 | A kind of liver-protection health-care drinks and preparation method thereof |
| CN109363011B (en) * | 2018-11-19 | 2021-12-14 | 惠州同富康生物科技有限公司 | Beverage with whitening, antioxidant, liver-protecting and sleep-helping functions and preparation method thereof |
| CN114365808A (en) * | 2021-12-10 | 2022-04-19 | 大连富森智能科技有限公司 | Method for preparing antrodia camphorata health-care beverage |
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Inventor after: Xu Zhenghong Inventor after: Qian Jianying Inventor after: Zhao Yucheng Inventor after: Shi Jinsong Inventor after: Lu Zhenming Inventor after: He Zhe Inventor after: Li Heng Inventor after: Xu Hongyu Inventor after: Xu Guohua Inventor after: Wang Yingyan Inventor before: Zhao Yucheng Inventor before: Shi Jinsong Inventor before: Lu Zhenming Inventor before: He Zhe Inventor before: Li Heng Inventor before: Xu Hongyu Inventor before: Xu Guohua Inventor before: Wang Yingyan Inventor before: Qian Jianying |
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