CN1456661A - Large scale zhangzhi fungus deep fermenting process - Google Patents

Large scale zhangzhi fungus deep fermenting process Download PDF

Info

Publication number
CN1456661A
CN1456661A CN 03131678 CN03131678A CN1456661A CN 1456661 A CN1456661 A CN 1456661A CN 03131678 CN03131678 CN 03131678 CN 03131678 A CN03131678 A CN 03131678A CN 1456661 A CN1456661 A CN 1456661A
Authority
CN
China
Prior art keywords
culture
camphor tree
tree sesame
production technique
technique according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN 03131678
Other languages
Chinese (zh)
Inventor
敖宗华
符慧子
邹锡良
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ao Zonghua
Fu Huizi
Zou Xiliang
Original Assignee
Jiangnan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangnan University filed Critical Jiangnan University
Priority to CN 03131678 priority Critical patent/CN1456661A/en
Publication of CN1456661A publication Critical patent/CN1456661A/en
Pending legal-status Critical Current

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A large-scale deep liquid fermenting process for preparing the mycelia of antrodia cinnamonea includes such steps as collecting the fungus of antrodia cinnamonea, liquid slant culture in shake flask, enlarging the culture, class-one seed culture, fermenting culture, and fermenting in (1000-5000)-L fermentor for 42 hr.

Description

Camphor tree sesame large-scale deep liquid fermentation production technique
Technical field
The present invention relates to a kind of camphor tree sesame large-scale deep liquid fermentation production technique.
Technical background
Camphor tree sesame (Antrodia camphorata) is a kind of famous Chinese medicine in Taiwan.Wild sporophore growth is at the rotten heartwood inwall of Taiwan protection seeds Cinnamomum kanahirai hay tree trunk, or the surface of the dark humidity of the Cinnamomum kanahirai hay timber of withered lodging, is not easy to be found very much.The camphor tree sesame has long use history in Taiwan, it is most precious medicinal material that the aboriginal all is considered as the camphor tree sesame.Wild top grade under the psychological expectations effect that thing which is rare is dear, also when the river rises the boat goes up day by day for the price of natural camphor tree sesame, the most expensive wild fungus on the world market of being known as at present, market price does not often wait up to more than 10 ten thousand yuan of every jin New Taiwan Currencies to hundreds thousand of units, wildly top gradely reach 15000 dollars/kilogram, the king among the king in the medicinal material of can saying so.At present, can't artificial culture camphor tree sesame sporophore.In Taiwan, it is the protective foods of main raw material that existing in recent years tens companies develop with camphor tree sesame fermentation mycelium and zymophyte powder, can regulate physiological function, nourishing and fit keeping function, physical strength reinforcing, increase immunizing power, spiritual vigorous, enhance metabolism, reduce sense of fatigue.
Modern study finds that the resistance of oxidation of camphor tree sesame tunning is very strong.Taiwan's scholars is investigated in the oxidation resistant ability of external protection normal people red blood corpuscle the Antrodia Camphorata mycelium aqueous extract.By water-soluble peroxidation base disalt acidifying 2, two (the 2-miaow base propane) [2 of 2 '-azo, 2 '-Azobis (2-amidinopropane) dihydrochloride, AAPH] the red blood corpuscle oxidation haemolysis and the lipid/protein peroxidation that are caused suppressed in the mode that a kind of time and concentration rely on by Antrodia Camphorata mycelium.Antrodia Camphorata mycelium also can stop the loss of oxidation resistant gsh (GSH) and Triphosaden (ATP) in the red blood corpuscle kytoplasm.And the infringement of the human endothelial cell that is caused by AAPH also can be suppressed by Antrodia Camphorata mycelium.Interesting is that Antrodia Camphorata mycelium shows the significant cytotoxicity of leukemia HL-60 cell, but human endothelial cell there is not influence.These results show that Antrodia Camphorata mycelium has anti-peroxidation and anticancer composition.The camphor tree sesame substratum dry-matter (DMCM) that Taiwan's scholars is also cultivated deep liquid, the resistance of oxidation, the radical scavenging activity that leach the biologically active substance of different solvents extract in thing (DMF) and the mycelium have been done research.DMF shows the strongest inhibition to lipid peroxidation, and this activity and concentration have functional relation.When 0.2mg/mL, its oxidation resistant activity can be identical with butylated hydroxy anisole (BHA).The resistance of oxidation of mycelium n-hexane extract is minimum, otherwise other mycelium extracts have some to suppress the activity of lipid peroxidation.DMF and mycelium water extract (WEM) show significant free radical scavenging activity.The anti-oxidant activity that leaches thing and mycelium extract is relevant with albumen/polysaccharide ratio in contained polyphenol, total triterpene and the total polysaccharides.Find that in different model researchs the resistance of oxidation of DMCM is lower than DMF, this points out that antioxidant component main among the DMF is mycelial secondary metabolite.Above result shows that DMF can be developed to chemical protector and be used for the treatment of and the free radical diseases associated.
In recent years discover that Crude polysaccharides has the activity of strong anti-hepatitis B virus surface antigen in camphor tree sesame sporophore and the liquid submerged fermentation mycelium, the activity of 50ug/ml polysaccharide concentration hepatitis B virus resisting (HBV) surface antigen is better than the dosage of alpha-interferon 1000U/ml.And the polysaccharide that camphor tree sesame sporophore and some strain liquid submerged fermentation mycelium produce also has the antigenic activity of hepatitis B virus resisting e.This is to find first to derive from that polysaccharide has in the anti-HBV activity of cell levels in the edible fungus.
At present, the artificial culture of camphor tree sesame sporophore not success as yet.The plurality kinds of health care food of selling in Taiwan Province is almost solution fermentation production.China's Mainland relevant camphor tree sesame liquid fermenting patent only has one, and (publication number: CN1309176A), its fermentation is to shake a bottle level by the application of food Industry in Taiwan Institute of Development Studies.Mentioning in Taiwan Province agricultural commission in 2000 the plan of science and technology achievement Report, with 5 liters of fermentor cultivation, is 1.712g/L at the 259.5h mycelial yield.At present, the paper and the patent report that still do not have camphor tree sesame large-scale deep liquid fermentation process aspect.
Summary of the invention
The objective of the invention is to produce Antrodia Camphorata mycelium is target, invents a kind of camphor tree sesame large-scale deep liquid fermentation process of Cheap highly effective, for the large-scale deep liquid fermentation production of camphor tree sesame from now on lays the first stone.
Realize technical scheme of the present invention: with camphor tree sesame fungi is starting strain, adopts slant strains to carry out liquid shaking bottle cultivation, liquid shaking bottle enlarged culturing, first order seed cultivation and fermentation culture.This bacterial strain is available from Jinxiang, Shandong fungal studies institute.
Realize that concrete steps of the present invention are as follows:
(1) takes out slant strains and insert in the fresh slant medium of preparing, culture temperature 25-33 ℃, cultivated 240-312 hour.
(2) slant strains in the step 1 is transferred the shaking in the bottle of shake-flask culture base is housed, 25-33 ℃ of cultivation, rotating speed 120-180rpm/min cultivates the enlarged culturing of shaking bottle after 30-48 hour.
(3) bottle bacterial classification that shakes in the step 2 is transferred and carried out enlarged culturing again into the bottle that shakes that the shake-flask culture base is housed, 25-33 ℃ of cultivation, rotating speed 120-180rpm/min cultivates after 20-28 hour the access first class seed pot and cultivates, and inoculum size is 4-10%.
(4) bottle bacterial classification that shakes in the step 3 is transferred one and is equipped with in the first class seed pot of seed culture medium, keep warm 25-33 ℃ in jar, tank pressure 0.5-0.9kPa, stir speed (S.S.) 80-150rpm/min, air flow 1: 0.3-0.5, fermented 30-48 hour, the fermentor tank of transferring is then cultivated, and inoculum size is 4-10%.
(5) bacterial classification in the first class seed pot is transferred one be equipped with in the fermentor tank of fermention medium, keep jar warm 25-33 ℃, tank pressure 0.5-0.9kPa, stir speed (S.S.) 80-150rpm/min, air flow 1: 0.3-0.5 fermented 35-48 hour.When being reduced to 0.05% left and right sides, reducing sugar content puts jar.
The prescription of slant medium is (g/100ml) among the present invention: potato 15-20, glucose 1-2, peptone 0.1-1, KH 2PO 40.1-0.5, MgSO 40.01-0.1 agar 1.5-2.5 adds water and is settled to volume requiredly, the pH value is 5.0.
The shake-flask culture base is (g/100ml) with shaking bottle prescription of enlarged culturing base among the present invention: glucose 2-4, peptone 0.5-1.5, analysis for soybean powder 0.5-2, wheat bran juice 1.5-2.5, KH 2PO 40.1-0.5, MgSO 40.01-0.1, adding water and be settled to volume requiredly, the pH value is 5.0.
The first order seed culture medium prescription of 100L fermentor tank is (g/100ml) among the present invention: glucose 2-4, peptone 0.5-1.5, analysis for soybean powder 0.5-2, wheat bran juice 0.1-0.5, KH 2PO 40.1-0.5, MgSO 40.05-0.15, adding water and be settled to volume requiredly, the pH value is 5.0.
The fermentative medium formula of 1500L fermentor tank is (g/100ml) among the present invention: glucose 2-6, peptone 0.5-1.5, analysis for soybean powder 0.5-2, wheat bran juice 0.3-0.8, KH 2PO 40.1-0.5, MgSO 40.05-0.15, adding water and be settled to volume requiredly, the pH value is 5.
The shake-flask culture base is 1: 5 with shaking bottle volumetric ratio in the step 2 of the present invention, and the shake-flask culture base is 1: 5 with shaking bottle volumetric ratio in the step 3.
The volume of first class seed pot is 100-300L in the step 4 of the present invention, and the fermention medium that is equipped with in this first class seed pot should be 50-180L mutually; The volume of fermentor tank is 1000-5000L in the step 5, and the fermention medium that is equipped with in this fermentor tank should be 500-3500L mutually.
Jar standard of putting of the present invention is that fermented liquid begins the thickness that becomes, and reducing sugar content is reduced to about 0.05%, and microscopy finds that mycelium senesces.
Beneficial effect of the present invention: the present invention is applicable to that large-scale deep liquid fermentation produces the camphor tree sesame, has solved the scale operation of camphor tree sesame and has cultivated a difficult problem of amplifying, and is significant for the development of camphor tree sesame suitability for industrialized production from now on and relevant application industry.The tunning color that the present invention obtains after fermentation ends is bright-coloured rose, gives an attractive smell, and fermented liquid has bitter taste.Dry mycelium is rose.The dry weight of fermentation mycelium is 1-1.5%, and polysaccharide content is 6-8% in the fermentation mycelium.The resistance of oxidation of this fermentation mycelium dry powder is very strong.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
Embodiment 1:
In slant medium, inoculate Antrodia Camphorata mycelium, 28 ℃ of culture temperature, incubation time 264 hours.Fresh slant strains access is equipped with in the 500ml triangular flask of 100ml substratum, and 28 ℃ of shaking tables were cultivated shaking speed 150rpm/min 40 hours.Then gained is shaken transfer 500ml triangle that the 100ml substratum is housed of bottle bacterial classification and shake and carry out enlarged culturing in the bottle, 28 ℃ of shaking tables were cultivated shaking speed 150rpm/min 22 hours.During the bacterial classification 2200ml that triangular flask is enlarged transfers the 100L first class seed pot that the 50L seed culture medium is housed then, keep 28 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100 rpm/min, air flow 1: 0.5, fermentation time 45 hours.The first class seed pot bacterial classification is transferred in the 1500L fermentor tank that the 800L fermention medium is housed, keep 28 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, air flow 1: 0.5, fermentation time 42 hours.
The slant medium composition is (g/100ml) in the present embodiment 1: potato 15, glucose 2, peptone 0.5, KH 2PO 40.1, MgSO 40.05 agar 2 adds water and is settled to volume requiredly, the pH value is 5.0.
The shake flask fermentation substratum is (g/100ml) with shaking bottle expansion fermention medium composition in the present embodiment 1: glucose 3, peptone 1, analysis for soybean powder 1, wheat bran juice 2, KH 2PO 40.2, MgSO 40.1, adding water and be settled to volume requiredly, the pH value is 5.0.
100L first class seed pot substratum composition is (g/100ml) in the present embodiment 1: glucose 3, peptone 1, analysis for soybean powder 1, wheat bran juice 0.2, KH 2PO 40.2, MgSO 40.1, adding water and be settled to 50L, the pH value is 5.0.
The culture medium prescription of 1500L fermentor tank is (g/100ml) in the present embodiment 1: glucose 5, peptone 1.2, analysis for soybean powder 1.2, wheat bran juice 0.5, KH 2PO 40.25, MgSO 40.125, adding water and be settled to 800L, the pH value is 5.0.
The fermentation ends standard is in the present embodiment: fermented liquid begins the thickness that becomes, and reducing sugar content is reduced to 0.05%, and microscopy finds that mycelium senesces.

Claims (9)

1, a kind of camphor tree sesame liquid submerged fermentation production technique, it is characterized in that: with camphor tree sesame fungi is starting strain, adopts slant strains to carry out liquid shaking bottle cultivation, liquid shaking bottle enlarged culturing, first order seed cultivation and fermentation culture.
2, camphor tree sesame liquid submerged fermentation production technique according to claim 1 is characterized in that: being starting strain available from Jinxiang, Shandong fungal studies institute camphor tree sesame fungi.
3, camphor tree sesame liquid submerged fermentation production technique according to claim 1 is characterized in that: it is (g/100ml) that described slant strains substratum is formed: potato 15-20, glucose 1-2, peptone 0.1-1, KH 2PO 40.1-0.5, MgSO 40.01-0.1 agar 1.5-2.5 adds water and is settled to volume requiredly, the pH value is 5.0, and 25-33 ℃ of slant strains culture temperature cultivated 240-312 hour.
4, camphor tree sesame liquid submerged fermentation production technique according to claim 1 is characterized in that: it is (g/100ml) that the substratum of described shake-flask culture is formed: glucose 2-4, peptone 0.5-1.5, analysis for soybean powder 0.5-2, wheat bran juice 1.5-2.5, KH 2PO 40.1-0.5, MgSO 40.01-0.1, adding water and be settled to volume requiredly, the pH value is 5.0, and culture condition is: culture temperature 25-33 ℃, rotating speed 120-180rpm/min cultivated 30-48 hour, and switching is gone into to shake bottle and is carried out enlarged culturing then.
5, camphor tree sesame liquid submerged fermentation production technique according to claim 1 is characterized in that: it is (g/100ml) that the described substratum that shakes bottle enlarged culturing is formed: glucose 2-4, peptone 0.5-1.5, analysis for soybean powder 0.5-2, wheat bran juice 1.5-2.5, KH 2PO 40.1-0.5, MgSO 40.01-0.1, adding water and be settled to volume requiredly, the pH value is 5.0, and culture condition is: culture temperature 25-33 ℃, rotating speed 120-180rpm/min cultivated 20-28 hour, and switching is gone into first order seed and is cultivated then, and inoculum size is 4-10%.
6, camphor tree sesame liquid submerged fermentation production technique according to claim 1 is characterized in that: it is (g/100ml) that the substratum that described first order seed is cultivated is formed: glucose 2-4, peptone 0.5-1.5, analysis for soybean powder 0.5-2, wheat bran juice 0.1-0.5, KH 2PO 40.1-0.5, MgSO 40.05-0.15, adding water and be settled to volume requiredly, the pH value is 5.0, and culture condition is: culture temperature 25-33 ℃, stir speed (S.S.) 80-150rpm/min fermented 30-48 hour, and ferment tank is gone in switching then, and inoculum size is 4-10%.
7, camphor tree sesame liquid submerged fermentation production technique according to claim 1 is characterized in that: described fermentation culture, it is (g/100ml) that fermention medium is formed: glucose 2-6, peptone 0.5-1.5, analysis for soybean powder 0.5-2, wheat bran juice 0.3-0.8, KH 2PO 40.1-0.5, MgSO 40.05-0.15, adding water and be settled to volume requiredly, the pH value is 5.0, culture condition is: culture temperature 25-33 ℃, stir speed (S.S.) 80-150rpm/min fermented 35-48 hour.
8, camphor tree sesame liquid submerged fermentation production technique according to claim 1 is characterized in that: described fermentation culture, and can select the fermentor tank of 1000-5000L to carry out fermentation culture.
9, camphor tree sesame liquid submerged fermentation production technique according to claim 1, it is characterized in that: described fermentation culture, mycelium can reach 1-1.5% with dry weight basis in the fermented liquid.
CN 03131678 2003-06-03 2003-06-03 Large scale zhangzhi fungus deep fermenting process Pending CN1456661A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 03131678 CN1456661A (en) 2003-06-03 2003-06-03 Large scale zhangzhi fungus deep fermenting process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 03131678 CN1456661A (en) 2003-06-03 2003-06-03 Large scale zhangzhi fungus deep fermenting process

Publications (1)

Publication Number Publication Date
CN1456661A true CN1456661A (en) 2003-11-19

Family

ID=29411763

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 03131678 Pending CN1456661A (en) 2003-06-03 2003-06-03 Large scale zhangzhi fungus deep fermenting process

Country Status (1)

Country Link
CN (1) CN1456661A (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100371450C (en) * 2005-09-28 2008-02-27 莱阳农学院 Fatty acid extract of Antrodiacamphorata mycelium and its uses
CN101822170A (en) * 2010-05-06 2010-09-08 江南大学 Method for producing Anrodia camphorata mycelia based on solid-state surface culture
CN102047814A (en) * 2010-10-25 2011-05-11 青岛农业大学 Micro ventilation lime wood antrodia camphorate cultivation method
CN102172174A (en) * 2011-03-07 2011-09-07 江南大学 Antrodia camphorate quick liquid fermentation process based on asexual spores
CN102217487A (en) * 2011-03-31 2011-10-19 赵宗杰 Method for producing mycelia of Antrodia camphorata through deep liquid state fermentation
CN102283019A (en) * 2011-07-06 2011-12-21 佛山市顺德区今日景艺生物科技有限公司 Artificial rapid Antrodia camphorata breeding method
CN102321684A (en) * 2011-10-09 2012-01-18 江南大学 The biosynthetic fermentation process of a kind of promotion camphor tree sesame active result Antrodin C
CN103125270A (en) * 2013-02-28 2013-06-05 深圳市仁泰生物科技有限公司 High-yield antrodia cinnamomea mycelium fermentation method for triterpenoids
CN103141891A (en) * 2013-02-28 2013-06-12 江南大学 Preparation method of antrodia camphorata liver protection functional beverage
CN103613430A (en) * 2013-11-25 2014-03-05 浙江科技学院 Antrodia cinnamomea growth medium and preparation method and application thereof
CN104087631A (en) * 2014-07-15 2014-10-08 江苏阜丰生物科技有限公司 Method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation
CN104111302A (en) * 2014-08-13 2014-10-22 中山安荞生物科技有限公司 Method for measuring adenosine content of health-protection antrodia food
CN104186880A (en) * 2014-07-11 2014-12-10 福建农林大学 Antrodia mycelium flavor soft sweet and processing method thereof

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100371450C (en) * 2005-09-28 2008-02-27 莱阳农学院 Fatty acid extract of Antrodiacamphorata mycelium and its uses
CN101822170A (en) * 2010-05-06 2010-09-08 江南大学 Method for producing Anrodia camphorata mycelia based on solid-state surface culture
CN102047814A (en) * 2010-10-25 2011-05-11 青岛农业大学 Micro ventilation lime wood antrodia camphorate cultivation method
CN102172174A (en) * 2011-03-07 2011-09-07 江南大学 Antrodia camphorate quick liquid fermentation process based on asexual spores
CN102172174B (en) * 2011-03-07 2013-01-09 江南大学 Antrodia camphorate quick liquid fermentation process based on asexual spores
CN102217487A (en) * 2011-03-31 2011-10-19 赵宗杰 Method for producing mycelia of Antrodia camphorata through deep liquid state fermentation
CN102217487B (en) * 2011-03-31 2013-01-23 深圳市中科海外科技有限公司 Method for producing mycelia of Antrodia camphorata through deep liquid state fermentation
CN102283019B (en) * 2011-07-06 2016-08-03 佛山市顺德区今日景艺生物科技有限公司 The method of artificial rapid Antrodia camphorata breeding
CN102283019A (en) * 2011-07-06 2011-12-21 佛山市顺德区今日景艺生物科技有限公司 Artificial rapid Antrodia camphorata breeding method
CN102321684A (en) * 2011-10-09 2012-01-18 江南大学 The biosynthetic fermentation process of a kind of promotion camphor tree sesame active result Antrodin C
CN102321684B (en) * 2011-10-09 2013-04-17 江南大学 Fermentation method for promoting biosynthesis of antrodia cinnanonea active product of Antrodin C
CN103125270A (en) * 2013-02-28 2013-06-05 深圳市仁泰生物科技有限公司 High-yield antrodia cinnamomea mycelium fermentation method for triterpenoids
CN103141891A (en) * 2013-02-28 2013-06-12 江南大学 Preparation method of antrodia camphorata liver protection functional beverage
CN103141891B (en) * 2013-02-28 2018-01-19 江南大学 A kind of preparation method of antrodia camphorata liver protection functional beverage
CN103613430A (en) * 2013-11-25 2014-03-05 浙江科技学院 Antrodia cinnamomea growth medium and preparation method and application thereof
CN103613430B (en) * 2013-11-25 2016-04-13 浙江科技学院 A kind of Antrodia cinnamomea growth medium and its preparation method and application
CN104186880A (en) * 2014-07-11 2014-12-10 福建农林大学 Antrodia mycelium flavor soft sweet and processing method thereof
CN104087631A (en) * 2014-07-15 2014-10-08 江苏阜丰生物科技有限公司 Method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation
CN104087631B (en) * 2014-07-15 2017-04-19 江苏阜丰生物科技有限公司 Method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation
CN104111302A (en) * 2014-08-13 2014-10-22 中山安荞生物科技有限公司 Method for measuring adenosine content of health-protection antrodia food

Similar Documents

Publication Publication Date Title
CN105001009B (en) Antrodia camphorata richness terpene Mycelium culture base and application
KR100199920B1 (en) The method of culturing cordyceps
CN1456661A (en) Large scale zhangzhi fungus deep fermenting process
JP2008109929A (en) Culture method of antrodia camphorata
CN104450826B (en) A kind of coriolan extract and preparation method and application
CN104893992B (en) A kind of submerged fermentation method of Ganoderma Lucidum
CN104388322A (en) Solid state fermentation method of lipid-lowering Monascus purpureus Zhang-MP
WO2022100568A1 (en) Tricholoma matsutake and method for producing ergothioneine thereby
CN109370914B (en) Cordyceps sinensis mycelium powder and production method thereof
CN102816806B (en) Production method of grifolan selenium compound
TW201200143A (en) A method to improve cordycepin of Solid-state cultivation
CN110373332A (en) A kind of purple Monascus and its total ferment produce the method and application of Lovastatin
CN1259423C (en) Technology of producing morin aqueous extract and polysaccharide using large scale liquid submerged fermentation process
CN101463326B (en) Production method of Guangdong Cordyceps sinensismycelium
CN109517740A (en) One plant of monascus parpureus Went mutagenic fungi and a kind of water-solubility function red yeast rice and its preparation method and application
CN102037848B (en) Method for promoting and culturing effective officinal components of cordyceps nigrolla mycelium by adding yam
CN1220769C (en) Temilmyces fuliginosus large-scale liquid deep fermentation production process
CN1273010C (en) Technique for production of Tricholoma matsutake and polysaccharide thereof by large scale liquid submerged fermentation
CN106034738B (en) A kind of method with silkworm pupa as host artificial culture silkworm chrysalis cicada fungus
CN116355816A (en) Microorganism of fermented samara oil seed and blood lipid reducing composition thereof
CN109468232A (en) One plant of production feruloyl esterase thanks watt bulk bacteria and its application in white wine yeast
TW201226562A (en) Culture medium for culturing fruiting bodies of antrodia cinnamomea and method for culturing the same
CN1142179C (en) Multifunctional mycoprotein product for plant and its prepn
CN109055242A (en) A kind of monascus purpureus bacterial strain and its zymotechnique and application
CN105940956B (en) A kind of method with tussah silkworm chrysalis as host artificial culture silkworm chrysalis cicada fungus

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
ASS Succession or assignment of patent right

Owner name: AO ZONGHUA; FU HUIZI; ZOU XILIANG

Free format text: FORMER OWNER: JIANGNAN UNIVERSITY

Effective date: 20031229

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20031229

Applicant after: Ao Zonghua

Applicant after: Fu Huizi

Applicant after: Zou Xiliang

Applicant before: Jiangnan University

C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
AD01 Patent right deemed abandoned
C20 Patent right or utility model deemed to be abandoned or is abandoned