CN109370914B

CN109370914B - Cordyceps sinensis mycelium powder and production method thereof

Info

Publication number: CN109370914B
Application number: CN201811431180.3A
Authority: CN
Inventors: 马兰; 陈大伟; 刘小朋
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-11-28
Filing date: 2018-11-28
Publication date: 2021-07-27
Anticipated expiration: 2038-11-28
Also published as: CN109370914A

Abstract

The invention relates to the technical field of biological fermentation, and discloses cordyceps sinensis Ophiococcus sinesis mycelium powder and a production method thereof, wherein cordyceps sinensis is taken as a strain, inorganic selenium or/and organic selenium liquid is added into a culture medium for submerged fermentation to produce selenium-rich cordyceps sinensis mycelium, the yield of dry mycelium is 28-35g/L, the total selenium in the mycelium is 25-1350ug/g, the mass percent w/% of the organic selenium in the total selenium is more than 92%, the two mycelia also contain effective components such as cordycepic acid 4-8%, cordyceps polysaccharide 5-8%, adenosine 0.03-0.17%, and the mycelia are crushed to 80-100 meshes to prepare a finished product of the mycelium powder. The experimental research proves that the product has the functions of resisting oxidation, resisting cancer, preventing cancer, resisting fatigue, enhancing immunity, tonifying lung and kidney, protecting liver, detoxifying and the like, and has practicability as medicines, health-care food and selenium nutritional supplement.

Description

Cordyceps sinensis mycelium powder and production method thereof

Technical Field

The invention relates to the technical field of biological fermentation, in particular to selenium-rich cordyceps sinensis mycelium powder produced by liquid submerged fermentation and a production method thereof.

Background

Cordyceps sinensis (Cordyceps sinensis) lives in an alpine meadow with elevation of 3000-5200 m in Qinghai-Tibet plateau, and is an insect-bacterium complex. The relationship of the cordyceps sinensis is that after the cordyceps sinensis (Ophiococcus sinensis) infects the larva of the hepiales, the nutrition in the body of the larva is gradually converted into mycelium to fill the whole body tissue of the larva, only one layer of insect skin is remained, the shape of the larva is kept unchanged for several months in the low-temperature dry soil in winter, and the cordyceps sinensis is called when the temperature and humidity are proper in summer, the rod-shaped fruiting body (stroma) grows out from the head of the larva and the ground is exposed. The Cordyceps sinensis has also been named Hirsutella hepialid Chen et Shen, Hirsutella sinensis (Hirsutella sinensis), cephalosporium sinensis (cephalosporium dongchiasonia), and Strongylocentrospora sinensis (Synnemorpha), which are all of the same species and have been uniformly revised as Cordyceps sinensis (Ophiosporidyceps sinensis).

The cordyceps sinensis has special effects of regulating yin and yang and tonifying lung and kidney, and is the only traditional Chinese medicine capable of balancing and regulating yin and yang simultaneously. Modern pharmacology has proved that the cordyceps sinensis has the functions of immunoregulation, tumor resistance, oxidation resistance, aging resistance, fatigue resistance, stress response resistance, blood sugar and blood fat reduction, sex hormone-like action and the like. Therefore, the cordyceps sinensis is listed in the name of hirsutella hepialid Chen et Shen in the list of fungi for health products by the national food and drug administration (currently available).

Selenium is a trace element necessary for human body and is called 'vital miraculous element'. Many researches show that selenium has the effects of preventing and resisting cancers, enhancing immunity, resisting oxidation, preventing cardiovascular and cerebrovascular diseases, detoxifying heavy metal elements, expelling toxin, protecting livers and the like. Selenium is an important component of many enzymes in the human body. At present, selenium-rich products are classified into inorganic selenium-rich products and organic selenium-rich products. The toxicity of inorganic selenium is higher than that of organic selenium, and the activity is lower than that of organic selenium. The inorganic selenium-rich product is mainly sodium selenite, and the organic selenium-rich product can be classified into selenium compound product, microorganism selenium-rich product, plant selenium-rich product and animal selenium-rich product.

The invention uses cordyceps sinensis as a strain, and produces selenium-rich cordyceps sinensis mycelium powder by adding inorganic selenium or organic selenium liquid into a culture medium and carrying out submerged fermentation.

Because the temperature requirement of the cordyceps sinensis is extremely strict, the suitable growth temperature is 17-20 ℃, the growth of the cordyceps sinensis is seriously influenced when the temperature exceeds 22 ℃, and even death is caused when the temperature exceeds 25 ℃; moreover, the cordyceps sinensis grows slowly and has a long lag phase. The reported fermentation period of the prior art is as long as 15-20 days, and the report that the yield of mycelia is higher is 20.8g/L (the current research situation and the development trend of hirsutella sinensis in China in 7 th month 43 in 2014 by fermentation science and technology communication of royal imperial swallow and the like). The research results in the aspect of producing selenium-rich cordyceps sinensis mycelia through fermentation are less. Zhou nut Qiao and so on adopt cordyceps sinensis as selenium-rich carrier, brewer's grain as substrate liquid for submerged fermentation, the total selenium-rich amount of the cordyceps sinensis is 5808.20 mug/L (dry mass of hypha is multiplied by selenium content), the yield of dry hypha is 12.28g/L, and the total selenium amount of the dry hypha is 472 mug/g (Chinese brewing 2014). Chenhongwei and the like are used for breeding the high selenium-rich cordyceps sinensis strain by protoplast mutagenesis, the total selenium content reaches 125.8 mu g/g, and the thallus yield is 13.445g/L (2009, food and fermentation industry). The picrorhiza and the like adopt cordyceps sinensis to carry out selenium-rich liquid fermentation, the yield of thalli is 2.52 percent, and the selenium content is 7.90mg/Kg (2006.28 of Hubei university). Chinese patents in this respect are few, and CN201510992589.2 discloses a method for fermenting Cordyceps sinensis mycelia by using rice bran liquid, wherein the mycelia are enriched with selenium 32.97 μ g/g, and the yield of mycelia is 4.55 g/L. CN201610579616.8 discloses a culture medium of selenium-rich and low-lead and arsenic Chinese caterpillar fungus mycelium and a culture method thereof, wherein the selenium content in the produced mycelium is 52.01 mug/Kg, and the mycelium yield is not described.

In the prior art, the yield of deeply fermented selenium-rich cordyceps sinensis mycelia is low, and the defects of low utilization rate of inorganic selenium, low selenium content of products and low organic degree exist. Therefore, no report on the industrialization of liquid submerged fermentation selenium-rich cordyceps sinensis mycelium powder exists at present. The inventor overcomes the defects of the prior art by improving the screening and culturing methods of the culture medium, and obtains the research result that the yield of selenium-rich cordyceps sinensis mycelia is 35g/L, the total selenium content is 1350ug/g, wherein the organic selenium accounts for more than 92% of the total selenium content, and the utilization rate of inorganic selenium is more than 85%.

Disclosure of Invention

The invention aims to provide cordyceps sinensis mycelium powder and a production method thereof. Specifically, Cordyceps sinensis is taken as a strain, inorganic selenium or/and organic selenium liquid is added into a culture medium for submerged fermentation to produce selenium-rich Cordyceps sinensis mycelium powder, the yield of dry mycelium is 28-35g/L, the dry mycelium contains 625-1350ug/g total selenium, the organic rate is more than 92%, and the utilization rate of inorganic selenium in the culture medium is more than 85%; the dried mycelium also contains 4-8% of cordycepic acid, 5-8% of cordyceps polysaccharide and 0.03-0.17% of adenosine. Pulverizing the mycelium into 80-100 mesh powder to obtain selenium-rich Cordyceps sinensis mycelium powder.

The Cordyceps sinensis (Ophiococcus sinensis) strain is selected from CICC (China center for culture Collection of Industrial microorganisms) with the number of 14088.

The invention is realized by the following technical scheme:

a fermented Cordyceps sinensis mycelium powder, the Cordyceps sinensis mycelium powder is to regard Cordyceps sinensis as the bacterium, through adding sodium selenite or/and selenium-enriched yeast liquid to ferment and culture deeply in the culture medium, the fermented solution is through the solid-liquid separation and drying process, the dry mycelium obtained contains total selenium 625 plus material 1350ug/g, wherein the organic selenium is not lower than 92%; the mycelium is crushed into 80-100 meshes to prepare mycelium powder, the mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, and a small amount of mycelium is stained by methylene blue, and then the mycelium is placed under a microscope for oil microscopic examination to obtain the mycelium with isolated polynuclear nuclei.

A fermented Cordyceps sinensis mycelium powder, the Cordyceps sinensis mycelium powder is to regard Cordyceps sinensis as the bacterium, through adding sodium selenite or/and selenium-enriched yeast liquid submerged fermentation culture in the culture medium, the fermented solution is through the solid-liquid separation and drying process, the dry mycelium obtained contains total selenium 625 plus 1350ug/g, wherein the organic selenium is not lower than 92%, also contain 7-8% cordycepic acid, 5-8% Cordyceps sinensis polysaccharide and 0.03-0.17% adenosine; pulverizing the mycelium into 80-100 mesh to obtain mycelium powder with gray yellow to yellow brown color, no fishy smell and no odor, and staining with methylene blue to obtain polynuclear mycelium under microscope.

A fermented Chinese caterpillar fungus mycelium powder is prepared by taking Chinese caterpillar fungus as a strain, adding sodium selenite or/and selenium-enriched yeast liquid into a culture medium for submerged fermentation culture, carrying out solid-liquid separation and drying on fermentation liquor to obtain dry mycelium, wherein the total selenium content of the mycelium is 1000 other drugs/g, the organic selenium content is not less than 92%, and the mycelium also contains 7-8% of cordycepic acid, 5-8% of cordyceps polysaccharide and 0.03-0.17% of adenosine.

The method for culturing the cordyceps sinensis mycelium powder comprises the following steps: slant strain culture, shake flask seed culture, first-stage seeding tank culture, second-stage seeding tank culture, fermentation tank culture, solid-liquid separation, drying, detection and crushing. The method comprises the following specific steps:

1. slant culture of bacterial

(1) The slant culture of the strain selects DCH culture medium of China industrial microorganism strain preservation management center, and the culture is calculated according to the weight volume ratio (%): 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone, 1.0 percent of agar powder and 5.6 to 6.0 percent of inorganic ion solution pH (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml). Subpackaging, sterilizing at 121 deg.C, preparing slant, inoculating activated strain to slant culture medium, and culturing at 15-18 deg.C for 30-40 days.

(2) Seed culture in shake flasks

Shake flask culture medium: in terms of weight to volume (%): 1.0-2.0% of glucose, 1.0-2.5% of corn flour, 0.5-1.5% of molasses and 1.0-6.0% of potato; silkworm chrysalis powder 2.0-3.0 wt% or live silkworm chrysalis 4.0-6.0 wt%, soybean sprout powder 0.5-1.5 wt%, yeast powder 0.2-1.0 wt%, and bran 0.5-1.5 wt%; 0.01 to 0.05 percent of magnesium sulfate, 0.02 to 0.1 percent of monopotassium phosphate and the balance of water. After the culture medium is subpackaged, the culture medium is sterilized at 121 ℃, the cultured slant seeds are inoculated on a shaking culture medium shaker after the temperature is reduced, the temperature is 17-19 ℃, and the rotation speed is 100-150rpm for 12-18 days.

(3) First-class seed tank seed culture

First-level seeding tank culture medium: in terms of weight to volume (%): 1.0 to 2.0 percent of glucose, 1.5 to 2.5 percent of corn flour or 1.5 to 2.0 percent of starch, 0.5 to 1.5 percent of molasses, 0 to 2.0 percent of potato, 1.5 to 3.5 percent of silkworm chrysalis meal, 0.5 to 1.5 percent of soybean cake meal or 0.5 to 1.5 percent of soybean sprout meal, 0.2 to 1.0 percent of yeast powder, 0.5 to 1.5 percent of bran and 0.2 to 1.0 percent of peptone; 0.01 to 0.05 percent of magnesium sulfate, 0.02 to 0.1 percent of monopotassium phosphate, 0 to 0.001 percent of sodium selenite and the balance of water. After the first-stage seeding tank culture medium is disinfected and cooled, inoculating the shake-flask seeds into the first-stage seeding tank in an inoculation amount of 5-10%, controlling the temperature to be 17-19 ℃, and ventilating for 1: 0.2-0.4vvm, and culturing for 6-8 days.

(4) Second-level seed tank seed culture

Secondary seed tank culture medium: in terms of weight to volume (%): 1.0 to 2.0 percent of glucose, 1.5 to 2.5 percent of corn flour or 1.5 to 2.0 percent of starch, 0.5 to 1.5 percent of molasses, 0 to 2.0 percent of potato, 1.5 to 3.5 percent of silkworm chrysalis meal, 0.5 to 1.5 percent of soybean cake meal or 0.5 to 1.5 percent of soybean sprout meal, 0.2 to 1.0 percent of yeast powder, 0.5 to 1.5 percent of bran and 0.2 to 1.0 percent of peptone; 0.01 to 0.05 percent of magnesium sulfate, 0.02 to 0.1 percent of monopotassium phosphate, 0 to 0.002 percent of sodium selenite and the balance of water. After the culture medium of the secondary seed tank is disinfected and cooled, inoculating seeds of the primary seed tank into the secondary seed tank in an inoculation amount of 10-20%, controlling the temperature to be 17-19 ℃, and ventilating for 1: 0.2-0.4vvm, and culturing for 6-8 days.

(5) Cultivation in fermenter

Fermentation tank culture medium: in terms of weight to volume (%): 1.0 to 2.0 percent of glucose, 1.5 to 2.5 percent of corn flour or 1.5 to 2.0 percent of starch, 0.5 to 1.5 percent of molasses, 0 to 2.0 percent of potato, 1.5 to 3.5 percent of silkworm chrysalis meal, 0.5 to 1.5 percent of soybean cake meal or 0.5 to 1.5 percent of soybean sprout meal, 0.5 to 1.0 percent of yeast powder or 0.5 to 2.5 percent of selenium-enriched yeast powder, 0.5 to 1.5 percent of bran and 0.2 to 1.0 percent of peptone; 0.01 to 0.05 percent of magnesium sulfate, 0.02 to 0.1 percent of monopotassium phosphate, 0.0002 to 0.01 percent of sodium selenite and the balance of water. After the culture medium of the fermentation tank is disinfected and cooled, inoculating the seeds of the secondary seed tank with the inoculation amount of 10-30%, controlling the temperature to be 17-19 ℃, and ventilating for 1: 0.2-0.4vvm, and culturing for 8-10 days;

(6) solid-liquid separation, drying, detection and crushing

Performing solid-liquid separation on the fermentation liquor put in the tank, drying the solid, and detecting the content of the components in the obtained dried mycelium: total selenium 625-; the mycelium is crushed into 80-100 meshes to prepare a mycelium powder finished product, and the physical properties of the finished product are as follows: the product is in the form of gray yellow to yellow brown powder, has slight fishy smell and no foreign odor, and can be stained with methylene blue, and then placed under microscope for oil microscope examination to obtain multi-core hypha.

The invention has the following positive effects:

1. the selenium-rich cordyceps sinensis mycelium powder with the excellent characteristics of cordyceps sinensis and selenium is obtained by organically combining trace element selenium and cordyceps sinensis, and the product not only contains the physiological active substances of cordyceps sinensis, but also contains more than 0.1% of organic selenium. Many researches have proved that compared with the common cordyceps sinensis product, the health care or treatment effect of the cordyceps sinensis product is better than that of a cordyceps sinensis mycelium powder single product and an organic selenium single product. Can simultaneously play the inherent and synergistic physiological effects of the cordyceps sinensis and the selenium by one dose. Has practicability both as a medicine, a health food and a selenium nutritional supplement.

2. In the process of culturing selenium-rich cordyceps sinensis mycelia, a seeding tank and sodium selenite domesticated culture gradual adaptation process is adopted, the inhibition of the increase of the amount of sodium selenite in a culture medium on the growth of the cordyceps sinensis mycelia is overcome, the total selenium content of the cordyceps sinensis mycelia reaches 1350 mu g/g under the condition that the yield of the mycelia is close to 3 percent (embodiment 10) is realized for the first time, the utilization rate of the fermented selenium-rich cordyceps sinensis mycelia on inorganic selenium exceeds 85 percent, and the organic degree exceeds 92 percent is realized for the first time. Overcomes the defects of the prior art and is suitable for industrialized production.

Detailed Description

Example 1

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. Preparing culture medium, packaging, sterilizing, and pouring the slant. The activated Cordyceps strain with CICC number 14088 is inoculated on slant culture medium, and cultured at 15-16 deg.C for 40 days.

Preparing shake flask seeds: shake flask culture medium: 1.5 percent of glucose, 2.0 percent of corn flour, 0.5 percent of molasses and 6.0 percent of potato; 2.5% of silkworm chrysalis meal, 1.0% of soybean sprout powder, 0.5% of yeast powder and 1.0% of bran; 0.02% of magnesium sulfate, 0.02% of monopotassium phosphate and the balance of water. Firstly, cutting potatoes into small pieces, slightly boiling and extracting the silkworm chrysalis meal, corn flour, wheat bran and a proper amount of water for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strains on a shaking culture base, culturing for 17 days at the temperature of 17-19 ℃ and at the speed of 100 rpm and 130 rpm.

Preparing seeds in a seeding tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of starch, 3.0% of silkworm chrysalis powder, 0.5% of soybean sprout powder, 0.5% of yeast powder, 0.5% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds with 10% of inoculum size at the temperature of 17-19 ℃, stirring at the rotation speed of 100 plus 120rpm, and ventilating for 1: 0.2-0.4vvm, and culturing for 8 days.

Culturing in a fermentation tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of starch, 3.0% of silkworm chrysalis powder, 0.5% of soybean cake powder, 0.5% of yeast powder, 0.5% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate, 0.0002% of sodium selenite and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean cake meal and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds into a seeding tank with the inoculation amount of 10% in a fermentation tank, carrying out stirring at the temperature of 17-19 ℃ and the stirring speed of 100-120rpm, and ventilating 1: 0.2-0.4vvm, and the growth is thick after 7 days of culture.

Downstream processing: putting the fermentation liquor in a tank for solid-liquid separation, drying the solid, and obtaining the dry mycelium with the yield of 34.2 kg/ton fermentation liquor. The total selenium content in the dried mycelium is 25ug/g, the organic selenium content is 24ug/g (the utilization rate of inorganic selenium is 93.9%, the organic rate is 96%), the cordycepic acid content is 71.60mg/g, the cordyceps polysaccharide content is 72.83mg/g, and the adenosine content is 0.41 mg/g. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder, which is gray yellow to brown powder, has slight fishy smell and no foreign odor, and is stained with methylene blue to obtain small amount of mycelia with multiple nuclei under microscope.

Example 2

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. Preparing culture medium, packaging, sterilizing, preparing slant, inoculating activated Cordyceps strain with CICC number 14088 to slant culture medium, and culturing at 17-18 deg.C for 35 days.

Preparing shake flask seeds: shake flask culture medium: 1.5 percent of glucose, 2.0 percent of corn flour, 1.0 percent of molasses and 6.0 percent of potato; 2.5% of silkworm chrysalis meal, 1.0% of soybean sprout powder, 0.5% of yeast powder and 1.0% of bran; 0.02% of magnesium sulfate, 0.02% of monopotassium phosphate and the balance of water. Firstly, cutting potatoes into small pieces, adding silkworm chrysalis meal, corn flour, wheat bran and a proper amount of water, slightly boiling and extracting for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strains on a shaking table for culture at the temperature of 17-19 ℃ and the temperature of 100-130rpm for 16 days.

Preparing seeds in a seeding tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 1.5 percent of corn flour, 3.0 percent of silkworm chrysalis meal, 0.5 percent of soybean sprout powder, 0.5 percent of yeast powder, 0.3 percent of peptone, 2.0 percent of bran, 0.02 percent of magnesium sulfate, 0.03 percent of monopotassium phosphate and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds with 10% of inoculum size at the temperature of 17-19 ℃, stirring at the rotation speed of 100 plus 120rpm, and ventilating for 1: 0.2-0.4vvm, and culturing for 8 days.

Culturing in a fermentation tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of corn flour, 3.0% of silkworm chrysalis powder, 0.5% of soybean cake powder, 0.5% of yeast powder, 0.3% of peptone, 2.0% of bran, 0.02% of magnesium sulfate, 0.03% of potassium dihydrogen phosphate, 0.0005% of sodium selenite and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean cake meal and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a seeding tank with the inoculation amount of 10% in a fermentation tank, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and the growth is thick after 8 days of culture.

Downstream processing: putting the fermentation liquor in a tank for solid-liquid separation, drying the solid, and obtaining the dry mycelium with the yield of 33.5 kg/ton fermentation liquor. The total selenium content in the dried mycelium is 63ug/g, the organic selenium content is 60ug/g (the utilization rate of inorganic selenium is 92.6%, the organic rate is 95.2%), the cordycepic acid content is 76.44mg/g, the cordyceps polysaccharide content is 73.13mg/g, and the adenosine content is 0.36 mg/g. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder, which is gray yellow to brown powder, has slight fishy smell and no foreign odor, and is stained with methylene blue to obtain small amount of mycelium which has multinuclear hyphae under microscope.

Example 3

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. Preparing culture medium, packaging, sterilizing, pouring inclined plane, inoculating activated Cordyceps strain with CICC number 14088 to the inclined plane culture medium, and culturing at 17-18 deg.C for 35 days.

Preparing shake flask seeds: shake flask culture medium: 1.8 percent of glucose, 2.0 percent of corn flour, 0.5 percent of molasses and 6.0 percent of potato; 6.0 percent of live silkworm chrysalis, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder and 1.0 percent of bran; 0.02% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. Cutting potato into small pieces, cutting live silkworm pupa into pieces, adding corn flour, wheat bran and a proper amount of water, boiling for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strain on a shaking table for culture at 17-19 ℃ and 130rpm for 14 days.

Preparing seeds in a seeding tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of starch, 3.5% of silkworm chrysalis powder, 0.5% of soybean sprout powder, 0.5% of yeast powder, 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds with 10% of inoculum size at the temperature of 17-19 ℃, stirring at the rotation speed of 100 plus 120rpm, and ventilating for 1: 0.2-0.4vvm, and culturing for 7 days.

Culturing in a fermentation tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 1.5 percent of starch, 3.5 percent of silkworm chrysalis meal, 0.5 percent of soybean sprout powder, 0.5 percent of yeast powder, 0.2 percent of peptone, 1.5 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.001 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a seeding tank with the inoculation amount of 10% in a fermentation tank, controlling the temperature to be 17-19 ℃, stirring at the rotation speed of 100 plus materials and 120rpm, and ventilating at the speed of 1: 0.2-0.4vvm, and the growth is thick after 8 days of culture.

Downstream processing: putting the fermentation liquor in a tank for solid-liquid separation, drying the solid, and obtaining the dry mycelium with the yield of 34.1 kg/ton fermentation liquor. The total selenium content in the dried mycelium is 125ug/g, the organic selenium content is 117ug/g (the utilization rate of inorganic selenium is 94.24%, the organic rate is 93.6%), the cordycepic acid is 69.07mg/g, the cordyceps polysaccharide is 78.45mg/g, and the adenosine is 0.50 mg/g. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder, which is gray yellow to brown powder, has slight fishy smell and no foreign odor, and is stained with methylene blue to obtain small amount of mycelium which has multinuclear hyphae under microscope.

Example 4

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. The activated Cordyceps strain with CICC number 14088 is inoculated on slant culture medium and cultured at 15-18 deg.C for 36 days.

Preparing shake flask seeds: shake flask culture medium: 1.8 percent of glucose, 2.0 percent of corn flour, 0.5 percent of molasses and 6.0 percent of potato; 2.5% of silkworm chrysalis meal, 1.0% of soybean sprout powder, 0.5% of yeast powder and 1.0% of bran; 0.02% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. Firstly, cutting potatoes into small pieces, adding silkworm chrysalis meal, corn flour, wheat bran and a proper amount of water, slightly boiling and extracting for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strains on a shaking table for culture at the temperature of 17-19 ℃ and the temperature of 100 plus 130rpm for 16.5 days.

Preparing seeds in a seeding tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of starch, 3.5% of silkworm chrysalis powder, 0.5% of soybean sprout powder, 0.5% of yeast powder, 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate, 0.001% of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds with 10% of inoculum size at the temperature of 17-19 ℃, stirring at the rotation speed of 100 plus 120rpm, and ventilating for 1: 0.2-0.4vvm, and culturing for 7 days.

Culturing in a fermentation tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of starch, 3.5% of silkworm chrysalis powder, 0.5% of soybean cake powder, 0.5% of yeast powder, 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate, 0.003% of sodium selenite and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean cake meal and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating a secondary seed tank with 20% of inoculum size to a fermentation tank, inoculating seeds in the secondary seed tank at the temperature of 17-19 ℃, stirring at the rotation speed of 100 plus materials and 120rpm, and ventilating at the speed of 1: 0.2-0.4vvm, and the growth is thick after 8 days of culture.

Downstream processing: and (5) putting the fermentation liquor in a tank for solid-liquid separation, and drying and crushing the solid to obtain cordyceps sinensis mycelium powder. The yield of dry mycelia was 33.6 kg/ton of fermentation broth. The total selenium content of dried mycelium is 365ug/g, organic selenium is 340ug/g, cordycepic acid is 63.77mg/g, Cordyceps polysaccharide is 75.43mg/g, and adenosine is 0.32 mg/g. The utilization rate of inorganic selenium is 89.7 percent, and the organic rate is 93.2 percent. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 5

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. Sterilizing, cooling, inoculating activated Cordyceps strain with CICC number 14088 to slant culture medium, and culturing at 15-18 deg.C for 35 days.

Preparing shake flask seeds: shake flask culture medium: 1.0% of glucose, 2.5% of corn flour, 1.0% of molasses and 2.0% of potatoes; 6.0 percent of live silkworm chrysalis, 0.5 percent of soybean sprout powder, 0.2 percent of yeast powder and 1.0 percent of bran; 0.01 percent of magnesium sulfate, 0.1 percent of monopotassium phosphate and the balance of water. Cutting potato into small pieces, cutting live silkworm pupa into pieces, adding corn flour, wheat bran and a proper amount of water, boiling for 30min, filtering, collecting filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strain on shaking culture medium, culturing at 17-19 deg.C and 130rpm for 12 days.

Preparing seeds in a first-level seed tank: the culture medium is as follows: 1.0% of glucose, 2.5% of corn flour and 1.5% of molasses; silkworm chrysalis powder 1.5%, soybean cake powder 1.5%, yeast powder 0.5%, bran 0.5%, peptone 1.0%; magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%; the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean cake meal and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating the filtrate into shake flask seeds with an inoculum size of 5%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus of 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 8 days.

Secondary seed tank seed culture: the culture medium comprises 1.0% of glucose, 2.5% of corn flour and 1.5% of molasses; silkworm chrysalis powder 1.5%, soybean cake powder 1.5%, yeast powder 0.5%, bran 0.5%, peptone 1.0%; 0.02% of magnesium sulfate, 0.05% of monopotassium phosphate and 0.001% of sodium selenite; the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean cake meal and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating the filtrate into a first-stage seed tank seed with the inoculation amount of 10%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 8 days.

Culturing in a fermentation tank: the culture medium is as follows: 1.0% of glucose, 2.5% of corn flour and 1.5% of molasses; silkworm chrysalis powder 1.5%, soybean cake powder 1.5%, yeast powder 0.5%, bran 0.5%, peptone 1.0%; 0.02% of magnesium sulfate, 0.05% of monopotassium phosphate and 0.0045% of sodium selenite; the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean cake meal and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a secondary seed tank with the inoculation amount of 20%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 9 days.

Downstream processing: and (4) putting the fermentation liquor in a tank for solid-liquid separation, and drying and crushing the solid to obtain the selenium-rich cordyceps sinensis powder. The yield of mycelia was 28.6 kg/ton of fermentation broth. The selenium content of the bacterial powder is 625ug/g, the organic selenium is 585ug/g, the cordycepic acid is 83.66mg/g, the cordyceps polysaccharide is 83.66mg/g, and the adenosine is 0.30 mg/g. The utilization rate of selenium is 87.2 percent, and the organic rate is 93.6 percent. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 6

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. The activated Cordyceps strain with CICC number 14088 is inoculated on slant culture medium and cultured at 15-18 deg.C for 40 days.

Preparing shake flask seeds: shake flask culture medium: 1.5 percent of glucose, 1.5 percent of corn flour, 1.5 percent of molasses and 3.0 percent of potato; 4.0% of live silkworm chrysalis, 1.0% of soybean sprout powder, 1.0% of yeast powder and 1.5% of bran; 0.02% of magnesium sulfate, 0.05% of monopotassium phosphate and the balance of water. Cutting potato into small pieces, cutting live silkworm pupa into pieces, adding corn flour, wheat bran and a proper amount of water, boiling for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strain on a shaking table culture medium, culturing at 17-19 ℃ and 130rpm for 15 days.

Preparing seeds in a first-level seed tank: the culture medium comprises 1.5% of glucose, 2.0% of corn flour and 1.5% of molasses; 2.5% of silkworm chrysalis meal, 0.5% of soybean sprout powder, 1.0% of yeast powder, 1.0% of bran and 1.0% of peptone; 0.05% of magnesium sulfate, 0.1% of monopotassium phosphate and 0.001% of sodium selenite; the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating the filtrate into shake flask seeds with the inoculation amount of 10%, the temperature is 17-19 ℃, the stirring speed is 100 plus of 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 7 days.

Secondary seed tank seed culture: the culture medium comprises 1.5% of glucose, 2.0% of corn flour and 1.5% of molasses; 2.5% of silkworm chrysalis meal, 0.5% of soybean sprout powder, 1.0% of yeast powder, 1.0% of bran and 1.0% of peptone; magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, sodium selenite 0.002%; the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating a first-class seed with 15% of inoculum size, inoculating the first-class seed with the inoculum size of 17-19 ℃, stirring at the rotation speed of 100 plus materials at 120rpm, and ventilating for 1: 0.2-0.4vvm, and culturing for 8 days.

Culturing in a fermentation tank: the culture medium is glucose: 1.5 percent, corn flour 2.0 percent and molasses 1.5 percent; 2.5% of silkworm chrysalis meal, 0.5% of soybean sprout powder, 1.0% of yeast powder, 1.0% of bran and 1.0% of peptone; 0.05% of magnesium sulfate, 0.1% of monopotassium phosphate and 0.006% of sodium selenite; the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing, cooling, and inoculating the mixture into a secondary seed tank with an inoculation amount of 20%. The temperature is 17-19 ℃, the stirring speed is 100-: 0.2-0.4vvm, and the growth is thick after 8 days of culture.

Downstream processing: and (4) putting the fermentation liquor in a tank for solid-liquid separation, and drying and crushing the solid to obtain the cordyceps sinensis powder. The yield of the bacterial powder is 32.1 kg/ton fermentation liquor. The selenium content of the bacterial powder is 760ug/g, the organic selenium is 710ug/g, the cordycepic acid is 71.60mg/g, the cordyceps polysaccharide is 53.0mg/g, and the adenosine is 1.05 mg/g. The utilization rate of inorganic selenium is 89.2 percent, and the organic rate is 93.4 percent. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 7

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. The activated Cordyceps strain with CICC number 14088 is inoculated on slant culture medium and cultured at 15-18 deg.C for 38 days.

Preparing shake flask seeds: shake flask culture medium: 1.5 percent of glucose, 2.0 percent of corn flour, 1.0 percent of molasses and 5.0 percent of potato; 5.0% of live silkworm chrysalis, 1.0% of soybean sprout powder, 0.4% of yeast powder and 1.5% of bran; 0.01 percent of magnesium sulfate, 0.02 percent of monopotassium phosphate and the balance of water. Cutting potato into small pieces, cutting live silkworm pupa into pieces, adding corn flour, wheat bran and a proper amount of water, boiling for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strain on a shaking table for culturing at 17-19 ℃ and 130rpm for 16 days.

Preparing seeds in a first-level seed tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 2.0% of corn flour, 2.5% of silkworm chrysalis powder, 1.0% of soybean sprout powder, 0.5% of yeast powder, 1.0% of peptone, 1.0% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate, 0.001% of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds into a first-stage seed tank by 8 percent of inoculation amount, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 8 days.

Preparing seeds in a secondary seed tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 2.0 percent of corn flour, 2.5 percent of silkworm chrysalis meal, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder, 1.0 percent of peptone, 1.0 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.002 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a first-stage seed tank with the inoculation amount of 20%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 6 days.

Culturing in a fermentation tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 2.0 percent of corn flour, 2.5 percent of silkworm chrysalis meal, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder, 1.0 percent of peptone, 1.0 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.007 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a secondary seed tank with the inoculation amount of 25%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 10 days.

Downstream processing: and (5) putting the fermentation liquor in a tank for solid-liquid separation, and drying and crushing the solid to obtain cordyceps sinensis mycelium powder. The yield of the dry mycelia is 30.4 kg/ton fermentation liquor, the total selenium content of the dry mycelia is 855ug/g, the organic selenium content is 830ug/g, the cordycepic acid content is 48.79mg/g, the cordyceps polysaccharide content is 78.53mg/g, and the adenosine content is 0.88 mg/g. The utilization rate of inorganic selenium is 94.05 percent, and the organic rate is 97.1 percent. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 8

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. The activated Cordyceps strain with CICC number 14088 is inoculated into slant culture medium, and cultured at 15-18 deg.C for 37 days.

Preparing shake flask seeds: shake flask culture medium: 2.0 percent of glucose, 2.0 percent of corn flour, 0.5 percent of molasses and 6.0 percent of potato; 2.5% of silkworm chrysalis meal, 1.0% of soybean sprout powder, 0.5% of yeast powder and 1.0% of bran; 0.02% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. Firstly, cutting potatoes into small pieces, slightly boiling and extracting the silkworm chrysalis meal, corn flour, wheat bran and a proper amount of water for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strains on a shaking table for culture at the temperature of 17-19 ℃ and the rotation speed of 100 plus 130rpm for 18 days.

Preparing seeds in a first-level seed tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 1.5 percent of starch, 3.5 percent of silkworm chrysalis meal, 0.5 percent of soybean sprout powder, 0.5 percent of yeast powder, 0.2 percent of peptone, 1.5 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.001 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds into a first-stage seeding tank according to the inoculation amount of 10%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 6 days.

Preparing seeds in a secondary seed tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 1.5 percent of starch, 3.5 percent of silkworm chrysalis meal, 0.5 percent of soybean sprout powder, 0.5 percent of yeast powder, 0.2 percent of peptone, 1.5 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.002 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min by slight boiling, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating a first-stage seed into a second-stage seed tank by 20 percent of inoculation amount, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 8 days.

Fermentation culture in a fermentation tank: the culture medium is glucose: 1.5 percent of molasses, 1.0 percent of starch, 3.5 percent of silkworm chrysalis meal, 0.5 percent of soybean sprout powder, 0.5 percent of yeast powder, 0.2 percent of peptone, 1.5 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.008 percent of sodium selenite and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a secondary seed tank to a fermentation tank according to 30% of inoculation amount, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and growing thick after 9.5 days of culture.

Downstream processing: and (4) putting the fermentation liquor in a tank for solid-liquid separation, and drying and crushing the solid to obtain cordyceps sinensis mycelium powder. The yield of the dry mycelia is 29.8 kg/ton fermentation liquor, the total selenium content of the dry mycelia is 1120ug/g, organic selenium 1031ug/g, cordycepic acid 50.00mg/g, cordyceps polysaccharide 80.20mg/g and adenosine 0.89 mg/g. The utilization rate of inorganic selenium is 91.5 percent, and the organic rate is 92.1 percent. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 9

Preparing seeds in a first-level seed tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 2.0% of corn flour, 2.5% of silkworm chrysalis powder, 1.0% of soybean sprout powder, 0.5% of yeast powder, 1.0% of peptone, 1.0% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds into a first-stage seed tank by 8 percent of inoculation amount, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 8 days.

Preparing seeds in a secondary seed tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 2.0 percent of corn flour, 2.5 percent of silkworm chrysalis meal, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder, 1.0 percent of peptone, 1.0 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.002 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a first-stage seed tank with the inoculation amount of 20%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 8 days.

Culturing in a fermentation tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 2.0 percent of corn flour, 2.5 percent of silkworm chrysalis meal, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder, 1.0 percent of peptone, 1.0 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.009 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a secondary seed tank with the inoculation amount of 25%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 9.5 days.

Downstream processing: and (5) putting the fermentation liquor in a tank for solid-liquid separation, and drying and crushing the solid to obtain cordyceps sinensis mycelium powder. The yield of the dry mycelia is 28.2 kg/ton fermentation liquor, the total selenium content of the dry mycelia is 1250ug/g, the organic selenium is 1156ug/g, the cordycepic acid is 60.79mg/g, the cordyceps polysaccharide is 76.05mg/g, and the adenosine is 0.68 mg/g. The utilization rate of inorganic selenium is 85.9 percent, and the organic rate is 92.48 percent. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 10

Preparing shake flask seeds: shake flask culture medium: 2.0 percent of glucose, 2.0 percent of corn flour, 0.5 percent of molasses and 6.0 percent of potato; 2.5% of silkworm chrysalis meal, 1.0% of soybean sprout powder, 0.5% of yeast powder and 1.0% of bran; 0.02% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. Firstly, cutting potatoes into small pieces, adding silkworm chrysalis meal, corn flour, wheat bran and a proper amount of water, slightly boiling and extracting for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strains on a shaking table for culture at the temperature of 17-19 ℃ and the temperature of 100-130rpm for 16 days.

Preparing seeds in a first-level seed tank: the culture medium is as follows: 1.5 percent of glucose, 1.0 percent of molasses, 1.5 percent of starch, 3.5 percent of silkworm chrysalis meal, 0.5 percent of soybean sprout powder, 0.5 percent of yeast powder, 0.2 percent of peptone, 1.5 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.001 percent of sodium selenite and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds into a first-stage seeding tank according to the inoculation amount of 10%, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and culturing for 7 days.

Fermentation culture in a fermentation tank: the culture medium is glucose: 1.5 percent of molasses, 1.0 percent of starch, 3.5 percent of silkworm chrysalis meal, 0.5 percent of soybean sprout powder, 0.5 percent of yeast powder, 0.2 percent of peptone, 1.5 percent of bran, 0.01 percent of magnesium sulfate, 0.02 percent of potassium dihydrogen phosphate, 0.01 percent of sodium selenite and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a secondary seed tank to a fermentation tank according to 30% of inoculation amount, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and the growth is thick after 10 days of culture.

Downstream processing: and (5) putting the fermentation liquor in a tank for solid-liquid separation, and drying and crushing the solid to obtain cordyceps sinensis mycelium powder. The yield of the dry mycelia is 28.7 kg/ton fermentation liquor, the total selenium content of the dry mycelia is 1350ug/g, the organic selenium is 1251ug/g, the cordycepic acid is 61.00mg/g, the cordyceps polysaccharide is 70.20mg/g, and the adenosine is 0.78 mg/g. The utilization rate of inorganic selenium is 85.2 percent, and the organic rate is 92.7 percent. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 11

Preparing shake flask seeds: shake flask culture medium: 1.6 percent of glucose, 2.0 percent of corn flour, 0.8 percent of molasses and 6.0 percent of potato; 5.0 percent of live silkworm chrysalis, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder and 1.0 percent of bran; 0.02% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. Cutting potato into small pieces, cutting live silkworm pupa into pieces, adding corn flour, wheat bran and a proper amount of water, boiling for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strain on a shaking table culture medium, culturing at 17-19 ℃ and 130rpm for 17 days.

Preparing seeds in a seeding tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of starch, 3.5% of silkworm chrysalis powder, 0.5% of soybean sprout powder, 0.5% of yeast powder, 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds with 10% of inoculum size at the temperature of 17-19 ℃, stirring at the rotation speed of 100 plus 120rpm, and ventilating for 1: 0.2-0.4vvm, and culturing for 8 days.

Culturing in a fermentation tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 1.5% of starch, 3.5% of silkworm chrysalis meal, 0.5% of soybean sprout powder, 0.5% of selenium-rich yeast powder (total selenium 1000ug/g), 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds into a seeding tank with the inoculation amount of 20% in a fermentation tank, carrying out stirring at the temperature of 17-19 ℃ and the stirring speed of 100-120rpm, and ventilating 1: 0.2-0.4vvm, and the growth is thick after 9 days of culture.

Downstream processing: putting the fermentation liquor in a tank for solid-liquid separation, drying the solid, and obtaining the dry mycelium with the yield of 40.2 kg/ton fermentation liquor; the total selenium content of the dried mycelium is 95ug/g, and the total selenium content of the dried mycelium is 92 ug/g; the dried mycelium contains 70.67mg/g of cordycepic acid, 75.80mg/g of cordyceps polysaccharide and 0.39mg/g of adenosine. Pulverizing the dried mycelium, sieving with 80-100 mesh sieve to obtain Cordyceps mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 12

Preparing slant seeds: DCH slant medium: 5.0 percent of sucrose, 0.3 percent of yeast powder, 1.0 percent of tryptone and 1.0 percent of agar powder, and is prepared by using inorganic ion solution (0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate, 0.01 g of copper sulfate and distilled water with the constant volume of 1000 ml) with the pH value of 5.6-6.0. Preparing culture medium, packaging, sterilizing, pouring inclined plane, inoculating activated Cordyceps strain with CICC number 14088 to the inclined plane culture medium, and culturing at 17-18 deg.C for 38 days.

Preparing shake flask seeds: shake flask culture medium: 1.6 percent of glucose, 2.0 percent of corn flour, 1.0 percent of molasses and 6.0 percent of potato; 6.0 percent of live silkworm chrysalis, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder and 1.0 percent of bran; 0.02% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. Cutting potato into small pieces, cutting live silkworm pupa into pieces, adding corn flour, wheat bran and a proper amount of water, boiling for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strain on a shaking table for culturing at 17-19 ℃ and 130rpm for 16 days.

Preparing seeds in a seeding tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 2.0% of corn flour, 3.5% of silkworm chrysalis powder, 0.5% of soybean cake powder, 0.5% of yeast powder, 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, extracting silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water by slight boiling for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating shake flask seeds with 10% of inoculum size at the temperature of 17-19 ℃, stirring at the rotation speed of 100 plus 120rpm, and ventilating for 1: 0.2-0.4vvm, and culturing for 8 days.

Culturing in a fermentation tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 2.0% of corn flour, 3.0% of silkworm chrysalis meal, 1.0% of soybean cake meal, 1.0% of selenium-rich yeast powder (total selenium 1000ug/g), 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds in a seeding tank with the inoculation amount of 20% in a fermentation tank, wherein the temperature is 17-19 ℃, the stirring speed is 100 plus materials and 120rpm, and the ventilation is 1: 0.2-0.4vvm, and the growth is thick after 8 days of culture.

Downstream processing: putting the fermentation liquor in a tank for solid-liquid separation, drying the solid, and obtaining the dry mycelium with the yield of 36.1 kg/ton fermentation liquor. The total selenium content of the dried mycelium is 245ug/g, and the total selenium content of the dried mycelium is 236 ug/g; the dried mycelium contains 59.23mg/g of cordycepic acid, 63.95mg/g of cordyceps polysaccharide and 1.68mg/g of adenosine, and the dried mycelium is crushed and sieved by a sieve of 80-100 meshes to prepare cordyceps sinensis mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 13

Culturing in a fermentation tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 2.0% of corn flour, 3.0% of silkworm chrysalis meal, 1.0% of soybean cake meal, 2.0% of selenium-rich yeast powder (total selenium 1000ug/g), 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate, 0.003% of sodium selenite and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds into a seeding tank with 15% of inoculum size to a fermentation tank, carrying out 17-19 ℃ of temperature, stirring at a rotation speed of 100-120rpm, and ventilating at a speed of 1: 0.2-0.4vvm, and the growth is thick after 8 days of culture.

Downstream processing: putting the fermentation liquor in a tank for solid-liquid separation, drying the solid, and obtaining the dry mycelium with the yield of 36.1 kg/ton fermentation liquor. The total selenium content of the dried mycelium is 740ug/g, and the organic selenium content is 715 ug/g; the dried mycelium contains 69.30mg/g of cordycepic acid, 56.92mg/g of cordyceps polysaccharide and 1.08mg/g of adenosine, and the dried mycelium is crushed and sieved by a sieve of 80-100 meshes to prepare cordyceps sinensis mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Example 14

Preparing shake flask seeds: shake flask culture medium: 1.6 percent of glucose, 2.0 percent of corn flour, 1.0 percent of molasses and 6.0 percent of potato; 6.0 percent of live silkworm chrysalis, 1.0 percent of soybean sprout powder, 0.5 percent of yeast powder and 1.0 percent of bran; 0.02% of magnesium sulfate, 0.04% of monopotassium phosphate and the balance of water. Cutting potato into small pieces, cutting live silkworm pupa into pieces, adding corn flour, wheat bran and a proper amount of water, boiling for 30min, filtering, taking filtrate, adding other components, subpackaging, sterilizing, cooling, inoculating slant strain on a shaking table culture medium, culturing at 17-19 ℃ and 130rpm for 17 days.

Culturing in a fermentation tank: the culture medium comprises 1.5% of glucose, 1.0% of molasses, 2.0% of corn flour, 3.0% of silkworm chrysalis meal, 1.0% of soybean cake meal, 2.5% of selenium-rich yeast powder (total selenium 1000ug/g), 0.2% of peptone, 1.5% of bran, 0.01% of magnesium sulfate, 0.02% of potassium dihydrogen phosphate and the balance of water. Firstly, carrying out micro-boiling extraction on silkworm chrysalis meal, corn flour, wheat bran, soybean sprout powder and a proper amount of water for 30min, filtering, taking filtrate, adding other components, sterilizing and cooling, inoculating seeds into a seeding tank with 15% of inoculum size to a fermentation tank, carrying out 17-19 ℃ of temperature, stirring at a rotation speed of 100-120rpm, and ventilating at a speed of 1: 0.2-0.4vvm, and the growth is thick after 8 days of culture.

Downstream processing: putting the fermentation liquor in a tank for solid-liquid separation, drying the solid, and obtaining the dry mycelium with the yield of 38.0 kg/ton fermentation liquor. The total selenium content of the dried mycelium is 525ug/g, and the organic selenium content is 506 ug/g; the dried mycelium contains 70.35mg/g of cordycepic acid, 56.09mg/g of cordyceps polysaccharide and 0.78mg/g of adenosine, and the dried mycelium is crushed and sieved by a sieve of 80-100 meshes to prepare cordyceps sinensis mycelium powder. The mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, is taken a little, is dyed by methylene blue, and is placed under a microscope for oil microscope examination to show multinuclear hyphae.

Claims

1. A fermented Chinese caterpillar fungus mycelium powder is characterized in that the Chinese caterpillar fungus mycelium powder takes Chinese caterpillar fungus as a strain, sodium selenite or/and selenium-enriched yeast liquid is added into a culture medium for submerged fermentation culture, fermentation liquor is subjected to solid-liquid separation and drying, the obtained dry mycelium contains 625-1350ug/g total selenium, wherein organic selenium is not less than 92%, the mycelium is crushed into 80-100 meshes to prepare the mycelium powder, the mycelium powder is gray yellow to yellow brown, has slight fishy smell and is free of peculiar smell, and after a small amount of mycelium is stained with methylene blue, the mycelium with separated polynuclear hyphae can be seen under a microscope by oil microscope examination.

2. A fermented Cordyceps sinensis mycelium powder is characterized in that Cordyceps sinensis is used as a strain, sodium selenite or/and selenium-enriched yeast liquid is added into a culture medium for submerged fermentation culture, a fermentation liquid is subjected to solid-liquid separation and drying, the obtained dry mycelium contains 1000 plus selenium of 1350ug/g, wherein the organic selenium is not less than 92%, the mycelium is crushed into 80-100 meshes to prepare the mycelium powder, the mycelium powder is gray yellow to yellow brown, has slight fishy smell and is free of peculiar smell, and after a small amount of mycelium is stained with methylene blue, the mycelium is placed under a microscope for oil microscopic examination, and the existence of isolated polynuclear mycelium can be seen.

3. A fermented Cordyceps sinensis mycelium powder is characterized in that Cordyceps sinensis is used as a strain, sodium selenite or/and selenium-rich yeast liquid is added into a culture medium for submerged fermentation culture, a fermentation broth is subjected to solid-liquid separation and drying, the obtained dried mycelium contains 625-1350ug/g of total selenium, wherein organic selenium is not less than 92%, 7-8% of cordycepic acid, 5-8% of cordyceps polysaccharide and 0.03-0.17% of adenosine are also contained in the dried mycelium, the mycelium is crushed into 80-100 meshes to prepare the mycelium powder, the mycelium powder is gray yellow to yellow brown, has slight fishy smell and is free of peculiar smell, a small amount of mycelium powder is taken, and after methylene blue is added for dyeing, and then the mycelium powder is placed under a microscope to be detected by an oil microscope to have isolated multinuclear mycelium.

4. A fermented Cordyceps sinensis mycelium powder is characterized in that Cordyceps sinensis is used as a strain, sodium selenite or/and selenium-enriched yeast liquid is added into a culture medium for submerged fermentation culture, fermentation liquor is subjected to solid-liquid separation and drying, the obtained dried mycelium contains 1000-cake total selenium 1350ug/g, wherein organic selenium is not less than 92%, 7-8% of cordycepic acid, 5-8% of cordyceps polysaccharide and 0.03-0.17% of adenosine are also contained in the dried mycelium, the mycelium is crushed into 80-100 meshes to prepare the mycelium powder, the mycelium powder is gray yellow to yellow brown, has slight fishy smell and is free of peculiar smell, a small amount of the product is taken, and after methylene blue is added for dyeing, and then the mycelium powder is placed under a microscope to be detected by an oil microscope to have multinuclear mycelium.

5. The method for producing the fermented cordyceps sinensis mycelium powder according to any one of claims 1 to 4, comprising: slant strain culture, shake flask seed culture, first-stage seeding tank culture, second-stage seeding tank culture, fermentation tank culture, solid-liquid separation, drying, detection and crushing; the method comprises the following specific steps:

(1) slant culture of bacterial

The slant culture of the strain selects DCH culture medium of China industrial microorganism strain preservation management center, and the weight volume ratio is as follows: 5.0% of sucrose, 0.3% of yeast powder, 1.0% of tryptone, 1.0% of agar powder and inorganic ion solution; the inorganic ion solution is 0.5 g of magnesium sulfate, 1 g of monopotassium phosphate, 1 g of sodium chloride, 0.3 g of calcium chloride, 0.01 g of manganese sulfate, 0.01 g of zinc sulfate, 0.01 g of ferrous sulfate and 0.01 g of copper sulfate, and the volume of distilled water is up to 1000 ml, and the pH value is 5.6-6.0; subpackaging, sterilizing at 121 deg.C, preparing slant, inoculating activated strain to slant culture medium, and culturing at 15-18 deg.C for 30-40 days;

(2) seed culture in shake flasks

Shake flask culture medium: the weight volume ratio is as follows: 1.0-2.0% of glucose, 1.0-2.5% of corn flour, 0.5-1.5% of molasses and 2.0-6.0% of potato; silkworm chrysalis powder 2.5 wt% or live silkworm chrysalis 4.0-6.0 wt%, soybean bud powder 0.5-1.0 wt%, yeast powder 0.2-1.0 wt%, and bran 1.0-1.5 wt%; 0.01 to 0.02 percent of magnesium sulfate, 0.02 to 0.1 percent of monopotassium phosphate and the balance of water; after the culture medium is subpackaged, sterilizing at 121 ℃, cooling, inoculating the cultured slant seeds on a shaking culture medium shaker, culturing at 17-19 ℃ and at the rotation speed of 100-130rpm for 12-18 days;

(3) first-class seed tank seed culture

First-level seeding tank culture medium: the weight volume ratio is as follows: 1.0-1.5% of glucose, 1.0-2.5% of corn flour or 1.5% of starch, 1.0-2.0% of molasses, 0-2.0% of potato, 1.5-3.5% of silkworm chrysalis meal, 1.5% of soybean cake powder or 0.5-1.0% of soybean sprout powder, 0.5-1.0% of yeast powder, 0.5-1.5% of bran and 0.2-1.0% of peptone; 0.01-0.05% of magnesium sulfate, 0.02-0.1% of monopotassium phosphate, 0-0.001% of sodium selenite and the balance of water; after the first-stage seeding tank culture medium is disinfected and cooled, inoculating the shake-flask seeds into the first-stage seeding tank in an inoculation amount of 5-10%, controlling the temperature to be 17-19 ℃, and ventilating for 1: 0.2-0.4vvm, culturing for 6-8 days;

(4) second-level seed tank seed culture

Secondary seed tank culture medium: the weight volume ratio is as follows: 1.0-1.5% of glucose, 1.0-2.5% of corn flour or 1.5% of starch, 1.0-2.0% of molasses, 0-2.0% of potato, 1.5-3.5% of silkworm chrysalis meal, 1.5% of soybean cake powder or 0.5-1.0% of soybean sprout powder, 0.5-1.0% of yeast powder, 0.5-1.5% of bran and 0.2-1.0% of peptone; 0.01 to 0.05 percent of magnesium sulfate, 0.02 to 0.1 percent of monopotassium phosphate, 0.001 to 0.002 percent of sodium selenite and the balance of water; after the culture medium of the secondary seed tank is disinfected and cooled, inoculating seeds of the primary seed tank into the secondary seed tank in an inoculation amount of 10-20%, controlling the temperature to be 17-19 ℃, and ventilating for 1: 0.2-0.4vvm, culturing for 6-8 days;

(5) fermentation tank culture medium: the weight volume ratio is as follows: 1.0-1.5 percent of glucose, 2.0-2.5 percent of corn flour or 1.5 percent of starch, 1.0-1.5 percent of molasses, 0-2.0 percent of potato, 1.5-3.5 percent of silkworm chrysalis meal, 1.5 percent of soybean cake meal or 0.5-1.0 percent of soybean sprout powder, 0.5-1.0 percent of yeast powder or 0.5-2.5 percent of selenium-enriched yeast powder, 0.5-1.5 percent of bran and 0.2-1.0 percent of peptone; 0.01-0.05% of magnesium sulfate, 0.02-0.1% of monopotassium phosphate, 0.0045-0.01% of sodium selenite and the balance of water; after the culture medium of the fermentation tank is disinfected and cooled, inoculating the seeds of the secondary seed tank with the inoculation amount of 10-30%, controlling the temperature to be 17-19 ℃, and ventilating for 1: 0.2-0.4vvm, and culturing for 8-10 days;

(6) solid-liquid separation, drying, detection and crushing

Performing solid-liquid separation on fermentation liquor placed in a tank, drying the solid, detecting that the obtained dried mycelium contains 625-1350ug/g of total selenium, the organic selenium accounts for more than or equal to 92 percent of the total selenium by mass, and also contains 4-8 percent of cordycepic acid, 5-8 percent of cordyceps polysaccharide and 0.03-0.17 percent of adenosine, crushing the mycelium into 80-100 meshes to prepare mycelium powder, wherein the mycelium powder is gray yellow to yellow brown powder, has slight fishy smell and no peculiar smell, taking a small amount of the product, adding methylene blue for dyeing, and placing the product under a microscope for oil microscopic examination, and the separated multinuclear mycelium can be seen.