CN114467625B - Cultivation method of high-quality tussah cordyceps militaris - Google Patents
Cultivation method of high-quality tussah cordyceps militaris Download PDFInfo
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- CN114467625B CN114467625B CN202210064036.0A CN202210064036A CN114467625B CN 114467625 B CN114467625 B CN 114467625B CN 202210064036 A CN202210064036 A CN 202210064036A CN 114467625 B CN114467625 B CN 114467625B
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- tussah
- cordyceps militaris
- male
- cocoons
- live
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 50
- 238000012364 cultivation method Methods 0.000 title claims abstract description 8
- 241000382353 Pupa Species 0.000 claims abstract description 37
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 241000190633 Cordyceps Species 0.000 claims abstract description 9
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 claims abstract description 8
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims abstract description 7
- 229960005305 adenosine Drugs 0.000 claims abstract description 7
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 claims abstract description 7
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 7
- 239000005017 polysaccharide Substances 0.000 claims abstract description 7
- 150000004676 glycans Chemical class 0.000 claims abstract description 6
- 230000005058 diapause Effects 0.000 claims abstract description 5
- 238000012258 culturing Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 239000002985 plastic film Substances 0.000 claims description 2
- 229920006255 plastic film Polymers 0.000 claims description 2
- 230000003203 everyday effect Effects 0.000 claims 2
- 238000001035 drying Methods 0.000 claims 1
- 238000007873 sieving Methods 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 3
- 244000025254 Cannabis sativa Species 0.000 abstract description 2
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 241000233866 Fungi Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- 150000003833 nucleoside derivatives Chemical class 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- -1 cordycepin polysaccharide Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000014106 fortified food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 230000010016 myocardial function Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a cultivation method of high-quality tussah cordyceps militaris, which comprises the steps of inoculating liquid strain of cordyceps militaris into tussah living pupa to cultivate sporophore, wherein the tussah living pupa adopts male autumn tussah living pupa obtained by releasing diapause. Compared with the full adoption of the equal-quality female autumn tussah live pupa, the product has the advantages that the grass yield of the tussah cordyceps militaris product reaches more than 90%, the cordycepin content in the product is increased by 43.02%, the adenosine content is increased by 28.12%, the cordyceps polysaccharide content is increased by 20.76%, and meanwhile, the raw material price of the equal-quality product is greatly reduced, namely, the quality and economic benefit of the tussah cordyceps militaris are obviously improved.
Description
Technical Field
The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a cultivation method of high-quality tussah cordyceps militaris.
Background
Cordyceps militariscordyCepSmilitaris(L.)Link) The Cordyceps militaris is a model seed of Cordyceps genus of Clavipitaceae ascomycetes, is different from Cordyceps genus, is a dual-purpose fungus with high nutritive value and medicinal efficacy, and has effects of improving immunity, resisting tumor and improving myocardial function.
The tussah cordyceps militaris is characterized in that wild cordyceps militaris is separated and domesticated, and inoculated into a tussah living pupa body to cultivate sporophore, which is a complex of ergot cordyceps sporophore and tussah pupa. From the analysis of the effective components, the nutrient-enriched food not only contains nutrients such as protein, fat and the like, but also contains active components such as cordycepin, adenosine, cordyceps polysaccharide and the like. Cordycepin is a special nucleoside active substance in Cordyceps, and has anticancer, antibacterial, and antiviral effects; adenosine is an endogenous nucleoside distributed throughout human cells, participates in myocardial energy metabolism, and has physiological effects on the cardiovascular system and many systems and tissues of the body; cordyceps sinensis polysaccharide is considered as one of very good immune promoters, and can enhance the immunity of organisms. Therefore, the tussah cordyceps militaris can replace wild cordyceps sinensis, overcomes the defect of natural cordyceps sinensis resources, and provides raw materials for the fields of food, medicine and health products.
Research analysis shows that the individual proportion of male tussah pupa and female tussah pupa is about 6:4, the female tussah pupa is relatively larger and heavier than the male tussah pupa, the protein and fat content in the female tussah fresh pupa are about 12.1% and 4.2%, respectively, and the protein and fat content in the male tussah fresh pupa are about 10.86% and 6.4%, respectively. Female tussah pupas are favored because of large size, heavy mass and high protein content, and the mixed big tussah pupas (with more female proportions) and small tussah pupas (with more male proportions) obtained by purchasing male and female tussah pupas are classified in the retail market, and the selling price is about 38 yuan/jin and 33 yuan/jin respectively. Because protein is an important component of constituent cells, the traditional concept tends to select female tussah live pupa with high protein content as a host to cultivate tussah cordyceps militaris, however, the tussah pupa used for cultivating the tussah cordyceps militaris at present is a mixture of male tussah pupa and female tussah pupa because of higher selling price of large tussah pupa with more female proportion, and the quality of the cultivated tussah cordyceps militaris needs to be further improved.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides a cultivation method of high-quality tussah cordyceps militaris.
The technical scheme of the invention is as follows: a method for culturing high-quality tussah Cordyceps militaris comprises inoculating Cordyceps militaris liquid strain into tussah live pupa to culture fruiting body, wherein the tussah live pupa adopts male autumn tussah live pupa obtained by diapause relieving.
The invention breaks through the traditional concept, the male autumn tussah live pupa with low protein content is adopted as the tussah live pupa for cultivating the tussah cordyceps militaris, so that the grass yield of the tussah cordyceps militaris product reaches more than 90 percent, and compared with the female autumn tussah live pupa with equal mass, the cordycepin content in the product is increased by 43.02 percent, the adenosine content is increased by 28.12 percent, the cordycepin polysaccharide content is increased by 20.76 percent, and meanwhile, the raw material price of the product with equal mass is greatly reduced, namely, the quality and economic benefit of the tussah cordyceps militaris are both obviously improved.
Description of the embodiments
The invention relates to a cultivation method of high-quality tussah cordyceps militaris, which is characterized in that cordyceps militaris liquid strains are inoculated into tussah live pupa to cultivate sporophores according to the method in the prior art, and the method is different from the prior art in that male autumn tussah live pupa obtained by relieving diapause is adopted for hundred percent of tussah live pupa.
The specific method comprises the following steps:
1. preparation of Cordyceps militaris liquid strain culture medium
The formula of the culture medium comprises: potato 200g glucose 20g potassium dihydrogen phosphate 3g magnesium sulfate 1.5g vitamin B 2 10mg peptone 3g beef extract 5g water 1000ml pH 7; sterilizing at 121deg.C under 0.1-0.11MPa for 30 min.
2. Cordyceps militaris liquid strain preparation
Taking 0.3 cm of test tube stock of Cordyceps militaris from "Binggu No. 1" of Probiotechnological institute of agricultural sciences of Liaoning province 2 Inoculating size 6 pieces into 200 ml Cordyceps militaris liquid medium, standing at 25deg.C for 2d, shaking culturing at 150rpm for 3-5d, filtering with 40 mesh screen under aseptic conditionAnd (3) obtaining filtrate, and transferring the filtrate into a liquid culture medium according to the volume ratio of 5% for later use.
3. Cultivation of
Selecting autumn tussah cocoons for diapause removal, cutting cocoons, taking 1000 full male tussah cocoons at random, sterilizing with 75% ethanol surface, loading the cultured and filtered cordyceps militaris bacterial liquid into a continuous syringe under aseptic condition, taking sterilized male tussah cocoons, inoculating 0.4ml cordyceps militaris liquid strain from the 4 th body node on the back of the sterilized male tussah cocoons, placing the sterilized male tussah cocoons into a sterilized plastic tray, covering the sterilized plastic tray with a plastic film, culturing under the conditions of 19-22 ℃ and humidity of 60-80% and 600-1000lux, illuminating 12 hours per day, ventilating 2 times per day, and culturing for about 45 days to obtain mature tussah cordyceps militaris.
Comparison experiment:
the comparative example adopts the cultivation method of the embodiment of the invention, but randomly takes 1000 female tussah live pupas to replace 1000 male tussah live pupas.
The tussah cordyceps militaris obtained in the embodiment and the comparative example are respectively dried and crushed at 50-55 ℃ and then screened by a 40-mesh sieve, so that male and female tussah cordyceps militaris dry powder is obtained. Respectively taking the cordyceps sinensis dry powder of male and female tussah with equal mass, and analyzing the cordycepin, adenosine and cordyceps sinensis polysaccharide content according to the method of the prior art.
The test results are shown in the following table:
the results show that: the cordycepin content in the male tussah cordyceps militaris is increased by 43.02% compared with that in the female tussah cordyceps militaris; the adenosine content in the male tussah cordyceps militaris is increased by 28.12% compared with that in the female tussah cordyceps militaris; the content of Cordyceps polysaccharide in male tussah Cordyceps militaris is improved by 20.76% compared with female tussah Cordyceps militaris, and the quality of tussah Cordyceps militaris is improved.
Claims (1)
1. A preparation method of high-quality tussah cordyceps militaris dry powder is characterized in that cordyceps militaris liquid strains are inoculated into tussah living pupa to cultivate sporophores, and the preparation method is characterized in that: the tussah live pupa adopts male Qiu Zhacan obtained by relieving diapauseThe method comprises the steps of (1) drying and crushing living pupa, namely, obtaining male Qiu Zhacan cordyceps militaris, and sieving the dried and crushed cordyceps militaris with a 40-mesh sieve at 50-55 ℃ to obtain male tussah cordyceps militaris dry powder, wherein the cordycepin content in the male tussah cordyceps militaris dry powder is 5.02X10 3 mg/kg, adenosine content 1230 mg/kg, cordyceps polysaccharide content 4.13mg/kg;
the preparation method of the cordyceps militaris liquid strain comprises the following steps:
6 blocks of 0.3 cm are taken 2 Inoculating test tube mother strain of Cordyceps militaris No. 1 with size of Bingyu into 200 ml Cordyceps militaris liquid culture medium, standing at 25deg.C for 2d, shake culturing at 150rpm for 3-5d, filtering with 40 mesh screen under aseptic condition to obtain filtrate, and transferring filtrate into liquid culture medium at volume ratio of 5%;
the cultivation method for inoculating the cordyceps militaris liquid strain into the tussah living pupa to cultivate the fruiting body comprises the following steps:
selecting autumn tussah cocoons for diapause removal, cutting cocoons, taking male tussah live cocoons with full pupa quality, sterilizing the surfaces of the male tussah live cocoons by using 75% ethanol, filling the cultured and filtered cordyceps militaris bacterial liquid into a continuous syringe under a sterile condition, taking the sterilized male tussah live cocoons, inoculating 0.4ml of cordyceps militaris bacterial liquid strain from the 4 th body node on the back of the sterilized male tussah live cocoons, placing the sterilized male tussah live cocoons into a sterilized plastic tray, covering the sterilized male tussah live cocoons with a plastic film, culturing the male tussah live cocoons under the conditions of 19-22 ℃ and humidity of 60-80% and 600-1000lux, illuminating the male tussah live cocoons for 12 hours every day, ventilating the male tussah live cocoons for 2 times every day, and culturing the male tussah cocoons for 45 days.
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| CN202210064036.0A CN114467625B (en) | 2022-01-20 | 2022-01-20 | Cultivation method of high-quality tussah cordyceps militaris |
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| CN202210064036.0A CN114467625B (en) | 2022-01-20 | 2022-01-20 | Cultivation method of high-quality tussah cordyceps militaris |
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| CN114467625A CN114467625A (en) | 2022-05-13 |
| CN114467625B true CN114467625B (en) | 2024-02-02 |
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| CN112391295A (en) * | 2020-11-17 | 2021-02-23 | 辽宁省海洋水产科学研究院 | Preparation method of wild cordyceps militaris strain |
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2022
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112391295A (en) * | 2020-11-17 | 2021-02-23 | 辽宁省海洋水产科学研究院 | Preparation method of wild cordyceps militaris strain |
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