CN109105153A - A kind of high-efficient method for cultivating of cordyceps - Google Patents
A kind of high-efficient method for cultivating of cordyceps Download PDFInfo
- Publication number
- CN109105153A CN109105153A CN201811034151.3A CN201811034151A CN109105153A CN 109105153 A CN109105153 A CN 109105153A CN 201811034151 A CN201811034151 A CN 201811034151A CN 109105153 A CN109105153 A CN 109105153A
- Authority
- CN
- China
- Prior art keywords
- cordyceps
- tussah
- days
- cordyceps militaris
- inoculation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of high-efficient method for cultivating of cordyceps.This method is that will just the cocoon autumn tussah cocoon in duration of diapause of pupating is handled 6-8 days under the conditions of 19-28 DEG C, humidity 70-80%, in 0-4 DEG C after storage 17-23 days, tussah pupa living after cancelling poison, Cordyceps militaris strain 0.3-0.7ml is inoculated with from its 3rd~5 body segment of back, it is placed in vinyl disc, 45-50 days Cordyceps militaris are cultivated under the conditions ofs suitable temperature, humidity and illumination etc. mature to be harvested.The present invention makes the carelessness that goes out of cordyceps reach 89.2%, space utilization rate improves 56.7%, product quality, production efficiency and remarkable in economical benefits are promoted, technology guarantee is provided for the factorial production of cordyceps, and sufficient raw material is provided for food and Medicines and Health Product industry, market application prospect is wide.
Description
Technical field
The invention belongs to technical field of edible fungi cultivation, and in particular to a kind of high-efficient method for cultivating of cordyceps.
Background technique
Cordyceps militaris (cordyCepSmilitaris (L.) Link) also known as northern Chinese caterpillar Fungus are Ascomycotina Clavicipitaceae
The type sepecies of Cordyceps, belonged to cordyceps sinensis it is not of the same race, be it is a kind of with better nutritivity value and medicinal efficacy medicine food two
With fungi, the Ministry of Public Health was classified as new resource food in 2009.With raising immunity of organisms, antitumor and raising cardiac muscle
The effects of function.
Cordyceps are to be inoculated into tussah pupal cell living after wild cordyceps are separated, tamed and cultivate son
Entity, it is that the complex of Clavicipitaceae cordyceps militaris sporocarp and tussah chrysalis not only contains from the point of view of the analysis of its effective component
Nutrition abundant, but also contain cordycepin, cordycepic acid and Cordyceps sinensis polysaccharide isoreactivity ingredient.Wherein cordycepin is peculiar in cordyceps sinensis
Nucleoside active matter, it has the function of anticancer, antibacterial, antiviral etc.；Cordycepic acid (mannitol) can reduce cranium significantly
It presses, boost metabolism thus cerebral hemorrhage and cerebral thrombosis disease is made to be eased, and have the effects of diuresis and cough-relieving；Cordyceps sinensis polysaccharide
It is considered as one of extraordinary immunopotentiating agent on our times, body immunity can be enhanced.Therefore, cordyceps can
To substitute wild cordyceps sinensis, the deficiency of natural cs resource is compensated for, provides raw material for food and medicine field of health care products,
So having a vast market application prospect.However, although the artificial breeding techniques of cordyceps has succeeded at present,
But since the cultivate effect of cordyceps is affected by tussah pupal mass, training method and condition of culture etc., cause at present
Product goes out careless lower in cordyceps industrialization production.According to Chen Dan (edible mushroom, 2006 (3): 33-34) and Ma Rui (China
Animal and veterinary digest, 2013,9 (2): 97-110) report, using autumn commodity tussah chrysalis as raw material, using conventional vial training
The method of supporting, going out careless for cordyceps is respectively 64% and 65%, and careless and production efficiency is lower out for product, constrains toothed oak
The sound development of silkworm chrysalis Cordyceps sinensis industry.
Summary of the invention
In order to solve the problems, such as technique described above, the present invention is practical for production, from tussah chrysalis material quality, inoculation portion
Position, dosage of inoculation and condition of culture etc. carry out system research, make a set of cordyceps high-efficient method for cultivating, make toothed oak
The quality of silkworm chrysalis Cordyceps sinensis, product go out careless and production efficiency and significantly improve, and provide skill for the factorial production of cordyceps
Art guarantees, while greatly improving its industrial economy benefit, realizes increasing peasant income, industry synergy, and is Liaoning characteristic tussah
The sustainable health development of industry opened up a new way.
A kind of high-efficient method for cultivating of cordyceps of the present invention, specific steps include:
(1) the autumn tussah cocoon in duration of diapause of pupating of just cocooing handles 6-8 under the conditions of 19-28 DEG C, humidity 70-80%
It, is placed in spare after storing 17-23 days in 0-4 DEG C；
(2) Cordyceps militaris slant strains are inoculated into Cordyceps militaris culture medium and cultivate 3-5 days, wherein inoculative proportion are as follows:
Take 0.2-0.7cm2The 5-7 block Cordyceps militaris slant strains of size, are inoculated into 200ml Cordyceps militaris culture medium；
Filtrate is obtained after aseptically crossing 30-50 mesh, by volume 2.5-7.5% switching filtrate to fluid nutrient medium
Middle acquisition Cordyceps militaris strain is spare；
(3) after the tussah for taking step (1) to obtain pupa disinfection living, from its back 3-5 body segment, by syringe needle to pupa head side
To be pierced into about 1cm, inoculation step (2) preparation Cordyceps militaris strain 0.3ml-0.7ml, by the tussah chrysalis needle mouth after inoculation to
Upper tiling is placed in culture plane, then is covered with transparent membrane, in 18-25 DEG C of temperature, humidity 60-80%, intensity of illumination
It is cultivated under conditions of 600-1000lux, daily 8-12 hours illumination, daily ventilation 2 times, by culture in 45-50 days, fructification
Maturation can harvest and carry out deep processing.
For above-mentioned technical proposal, the formula of the Cordyceps militaris bacterium culture medium is as follows:
Potato 200g glucose 20g potassium dihydrogen phosphate 3g magnesium sulfate 1.5g vitamin B210mg peptone 3g beef extract
5g water 1000ml, pH value 7.
The autumn tussah cocoon cultivation temperature in duration of diapause for above-mentioned technical proposal, in preferred situation, in step (1)
It is 19-28 DEG C, optimal cultivation temperature is 25 DEG C；Incubation time most preferably 7 days；Optimal placement temperature is 2 DEG C, is stored 20 days
It is spare afterwards.
For above-mentioned technical proposal, in preferred situation, Cordyceps militaris slant strains are inoculated into Cordyceps militaris liquid in step (2)
Step in body culture medium, the time of culture are 4 days；
In preferred situation, in step (2), Cordyceps militaris slant strains are preferably sized as taking 0.3cm26 pieces be inoculated with,
And the screen to filtrate is that 40 meshes are best；
In preferred situation, in step (2), the volume ratio of the filtrate switching fluid nutrient medium after the screen to filtrate is 5%；It is right
Under above-mentioned technical proposal, preferred situation, tussah in step (3) pupa disinfection way living is generallyd use through 75% ethyl alcohol table
The mode that face sterilizes, cleans, drying.
For above-mentioned technical proposal, in preferred situation, tiling described in step (3) is placed in culture plane, then is used
Tussah chrysalis needle mouth after inoculation is usually placed in by the training method of transparent membrane covering upwards in the actual operation process
In vinyl disc after disinfection, vinyl disc is placed in culturing room, is covered on vinyl disc with transparent membrane.This mode has broken biography
The vial-type culture of system, takes full advantage of culture space, also substantially increases cultivation efficiency.
It at tussah the 4th body segment of pupa back living is most preferably to infuse in step (3) in preferred situation for above-mentioned technical proposal
Penetrate position；
For above-mentioned technical proposal, in preferred situation, the optimised quantity of inoculation Cordyceps militaris strain is in step (3)
0.5ml。
For above-mentioned technical proposal, in preferred situation, the tussah chrysalis after inoculation is put into vinyl disc in step (3) and is trained
It supports, the vinyl disc is long 53cm × wide 40cm × high 10cm, and every disk lays flat tussah chrysalis 200 or so.
For above-mentioned technical proposal, in preferred situation, vinyl disc is placed in culturing room by step in (3), vinyl disc
On usually covered with plastic cloth；
For above-mentioned technical proposal, in preferred situation, the more excellent cultivation temperature of the tussah chrysalis in step (3) after inoculation is
19-22℃；Optimum culturing temperature is 22 DEG C；Optimum illumination intensity is 800lux；Daily optimum illumination time is 12 hours.
The application of this method can make the product of cordyceps go out carelessness and reach 89.2%, and space utilization rate improves
56.7%, the yield and quality of Cordyceps militaris is improved, and production efficiency and remarkable in economical benefits are promoted, and is the work of cordyceps
The production of factory's metaplasia is laid a good foundation, and sufficient Cordyceps militaris raw material is provided for food and Medicines and Health Product industry.
Beneficial effect
1, according to the morphosis feature of tussah chrysalis, in Cordyceps militaris production process, different vaccination position and different vaccination
Dosage is affected to the yield and quality of Cordyceps militaris, and through development test, 0.5ml liquid is inoculated at the 4th body segment of tussah chrysalis back
The effect of body strain is preferable.
2, currently, the production of cordyceps generallys use vial (diameter 10cm × high 10cm) breeding method, each
5-6 Cordyceps militaris is cultivated in bottle, bottleneck covers with plastic foil, due to use vial cultivating method there are areas occupied more, space
Utilization rate is low, cumbersome and the problems such as be not easy the factorial production, so, production efficiency is lower；And use vinyl disc (long 53cm
× wide 40cm × high 10cm) cultivating method, technical operation is easy, and space utilization rate is high, is easy to the factorial production, is remarkably improved life
Produce efficiency.
3, the method for the invention, raw material resources are abundant, technique convenience, high production efficiency, good product quality, are suitble to work
Industry metaplasia produces；The product that this method can significantly improve cordyceps goes out carelessness, guarantees the yield and quality of Cordyceps militaris, makes up
The deficiency of natural cs resources, provides sufficient raw material for food and medicine field of health care products, before having a vast market application
Scape.
Specific embodiment
Following nonlimiting examples can with a person of ordinary skill in the art will more fully understand the present invention, but not with
Any mode limits the present invention.
Embodiment 1
Influence research of the different disposal temperature to tussah pupal mass
1.1 test method
Choose same breed, it is identical put in a suitable place to breed under the conditions of just taken off the autumn tussah cocoon in duration of diapause, 4 groups of test point, often
Group randomly selects 1000 tussah cocoons, handles 7 days under the conditions of 19,22,25,28 DEG C, humidity 60-70% respectively, then, cuts cocoon
Pupa, investigation morbidity pupa number and normal pupae number are taken, each test group tussah chrysalis disease incidence is calculated.
1.2 test result
1 is shown in Table by the test result that above-mentioned test method is carried out.
Influence of the 1 different disposal temperature of table to tussah chrysalis incidence
| Treatment temperature (DEG C) | Test cocoon number/ | Morbidity pupa number/ | Normal pupae number/ | Disease incidence/% |
| 19 | 1000 | 38 | 962 | 3.8 |
| 22 | 1000 | 54 | 946 | 5.4 |
| 25 | 1000 | 89 | 911 | 8.9 |
| 28 | 1000 | 92 | 908 | 9.2 |
It can see from 1 test result of table, 7 days, as the temperature rises, tussah chrysalis acted under the conditions of 19-28 DEG C
Disease incidence is also gradually increased, and 25 DEG C of disease incidence are 8.9%, and it is 9.2% that 28 DEG C of whens, which reach peak, but in view of temperature is excessively high right
The influence of tussah chrysalis pupa matter and energy problem, moreover, the tussah chrysalis disease incidence difference of 25 DEG C and 28 DEG C processing is smaller, so, it builds
It is more appropriate to discuss 25 DEG C of tussah cocoon treatment temperature selection.
Embodiment 2
Distinct methods processing tussah chrysalis Cordyceps militaris goes out careless situation and compares
1, test method
Cordyceps militaris Spawn incubation based formulas: potato 200g glucose 20g potassium dihydrogen phosphate 3g magnesium sulfate 1.5g dimension
Raw element B210mg peptone 3g beef extract 5g water 1000ml pH value 7.
121 DEG C of high pressure sterilizations, pressure reaches kept for 30 minutes after 0.1-0.11MPa after it is spare.
2, prepared by Cordyceps militaris strain
" ice valley 1 " Cordyceps militaris test tube stock that Dalian Biological Tech. Inst., Liaoning Academy of Agricultural Science's breeding is put on record
Take 0.3cm26 pieces of size, 25 DEG C of static gas wave refrigerator 2d in 200ml Cordyceps militaris culture medium are inoculated into, then in 150rpm/min
Shaken cultivation 3-5d is aseptically filtered to get filtrate with 40 mesh screens, by volume 5% switching filtrate to fluid nutrient medium
In for being inoculated with tussah chrysalis.
3, test procedure
The autumn tussah cocoon in duration of diapause will be just taken off to handle 7 days under the conditions of 25 DEG C, store in 0-4 DEG C of freezer
Tussah cocoon after 20 days cuts cocoon and takes pupa, takes tussah chrysalis 1000 at random, with 75% ethyl alcohol surface sterilization, aseptically will
It cultivates filtered Cordyceps militaris bacterium solution to be fitted into continuous syringe, the tussah chrysalis after cancelling poison is inoculated with from its 4th body segment of back
Cordyceps militaris strain 0.5ml is placed in the vinyl disc after sterilizing, is covered above with plastic foil, in 19-22 DEG C, humidity 60-
80%, it is cultivated under the conditions of 600-1000lux, daily illumination in 12 hours, daily ventilation 2 times, through cultivation in about 45 days, investigation statistics
Grow the tussah chrysalis number that fructification is more than 10.Meanwhile never heated processing, normal storage is in 0-4 DEG C of freezer
1000 tussah chrysalis are extracted in duration of diapause autumn tussah cocoon at random and carry out out grass test, test method is same as above, is investigated and analysed.
4, test result
1 is shown in Table by the test result that above-mentioned test method is carried out.
2 different disposal method of table goes out the influence of careless situation to tussah chrysalis
| Processing method | Test pupa number/ | Grow fructification pupa number/ | Carelessness/% out |
| 25 DEG C of processing | 1000 | 892 | 89.2 |
| Normal processing | 1000 | 674 | 67.4 |
It can see from 2 test result of table, the pupa living of the autumn tussah after being handled 7 days using 25 DEG C is material, each pupa inoculation
0.5ml Cordyceps militaris strain grows the tussah chrysalis that fructification is more than 10 after cultivating 45 days by above-mentioned Cordyceps militaris culture technique
It is 892, careless out is 89.2%；And the careless tussah chrysalis that goes out without saving under heat treatment normal condition is 674, it is careless out
It is 67.4%.Heat treatment goes out carelessness than the tussah chrysalis of routine preservation and improves 21.8%.
Embodiment 3
The influence that different vaccination position grows cordyceps
1, test method
Cordyceps militaris strain is prepared by 1 method of embodiment, the pupa living of the autumn tussah after handling 7 days using 25 DEG C is material, examination
Points 3 groups are tested, every group takes 1000 tussah chrysalis at random, and the 1st group of inoculation position is tussah chrysalis head about 1cm, and the 2nd group of inoculation position is
The 4th body segment of tussah chrysalis back (is pierced into about 1cm to pupa cephalad direction), and the 3rd group of inoculation position is tussah chrysalis tail portion about 1cm, inoculation
After be put into vinyl disc and cultivated in culturing room, Cordyceps militaris cultivating and managing method is identical with in example 1 1.3, after cultivating 45 days,
It analyzes and researches the growing states of each test group cordyceps.
2, test result
3 are shown in Table by the test result that above-mentioned test method is carried out
The influence that 3 different vaccination position of table grows cordyceps
| Inoculation position | Test pupa number/ | Fructification mean elements/pupa | Fructification average length/cm | Single pupa mean fresh/g |
| Pupa head | 1000 | 26.2 | 5.1 | 7.5 |
| The 4th body segment of pupa back | 1000 | 29.0 | 5.5 | 8.1 |
| Pupa tail portion | 1000 | 22.4 | 5.4 | 7.6 |
It can see from 3 result of table, inoculation position is affected to cordyceps growth.The 4th body of tussah chrysalis back
Section inoculation test group is above tussah chrysalis head in terms of fructification mean elements, fructification average length and single pupa mean fresh
With tussah chrysalis tail portion inoculation group, wherein fructification mean elements is the 1.1 of tussah chrysalis head and tussah chrysalis tail portion inoculation group respectively
With 1.3 times, single pupa mean fresh is 1.1 times of tussah chrysalis head and tussah chrysalis tail portion inoculation group.So with tussah chrysalis back
The quality and yield of Cordyceps militaris are best when at the 4th body segment as inoculation position.
Embodiment 4
The influence that Different vaccine dosage grows cordyceps
1, test method
Cordyceps militaris strain is prepared by 1 method of embodiment, the pupa living of the autumn tussah after handling 7 days using 25 DEG C is material, examination
Points 3 groups are tested, every group takes 1000 tussah chrysalis at random, and inoculation position is the 1st group of inoculation bacterium solution at the 4th body segment of tussah chrysalis back
Dosage is 0.3ml, the 2nd group of inoculation bacterium solution dosage is 0.5ml, the 3rd group of inoculation bacterium solution dosage is 0.7ml, is put into plastics after inoculation
It is cultivated in culturing room in disk, Cordyceps militaris cultivating and managing method is identical as in example 1 1.3, after cultivating 45 days, each examination of analyzing and researching
Test the growing state of group cordyceps.
2, test result
3 are shown in Table by the test result that above-mentioned test method is carried out
The influence that 4 Different vaccine dosage of table grows cordyceps
| Dosage of inoculation (ml) | Test pupa number/ | Fructification mean elements/pupa | Fructification average length/cm | Single pupa mean fresh/g |
| 0.3 | 1000 | 23.5 | 4.5 | 6.2 |
| 0.5 | 1000 | 29.0 | 5.5 | 8.3 |
| 0.7 | 1000 | 27.8 | 4.3 | 6.4 |
It can see from 4 result of table, Different vaccine dosage is affected to cordyceps growth.Dosage of inoculation
0.5ml test group be above in terms of fructification mean elements, fructification average length and single pupa mean fresh 0.3ml and
0.7ml dosage group.Wherein, fructification mean elements is 1.2 and 1.04 times of 0.3ml and 0.7ml dosage group respectively, and fructification is flat
1.2 and 1.3 times of equal length difference 0.3ml and 0.7ml dosage group, single pupa mean fresh is 0.3ml and 0.7ml dosage group
1.3 again.So the quality and yield of Cordyceps militaris are best when bacterium solution dosage of inoculation is 0.5ml.
Embodiment 5
Difference cultivates the influence that temperature grows cordyceps
1, test method
Cordyceps militaris strain is prepared by 1 method of embodiment, the pupa living of the autumn tussah after handling 7 days using 25 DEG C is material, examination
Points 3 groups are tested, every group takes 1000 tussah chrysalis at random, and inoculation position is at the 4th body segment of tussah chrysalis back, and inoculation bacterium solution dosage is equal
For 0.5ml, it is put into vinyl disc after inoculation and is cultivated in culturing room.Wherein, it is 19 DEG C, the 2nd group that the 1st group of tussah chrysalis, which cultivates temperature,
Tussah chrysalis cultivation temperature is 22 DEG C, the 3rd group of tussah chrysalis cultivation temperature is 25 DEG C, 1.3 in Cordyceps militaris cultivating and managing method and example 1
It is identical, after cultivating 45 days, the growing state for each test group cordyceps of analyzing and researching.
2, test result
5 are shown in Table by the test result that above-mentioned test method is carried out
The different influences cultivated temperature and cordyceps are grown of table 5
| Cultivation temperature (DEG C) | Test pupa number/ | Fructification mean elements/pupa | Fructification average length/cm | Single pupa mean fresh/g |
| 19 | 1000 | 23.2 | 4.1 | 7.1 |
| 22 | 1000 | 29.0 | 5.5 | 8.0 |
| 25 | 1000 | 20.0 | 3.5 | 5.5 |
It can see from 5 result of table, difference cultivates temperature and is affected to cordyceps growing state.Cultivating temperature is
It is 19 that 22 DEG C of test groups, which are above in terms of fructification mean elements, fructification average length and single pupa mean fresh and cultivate temperature,
DEG C and 23 DEG C of test groups.Wherein, fructification mean elements is 1.3 and 1.5 times of 19 DEG C and 23 DEG C test groups respectively, and fructification is flat
Equal length distinguishes 1.3 and 1.6 times of 19 DEG C and 23 DEG C test groups, and single pupa mean fresh is respectively 19 DEG C and 23 DEG C of test groups
1.1 and 1.5 times.So the quality and yield of cultivating Cordyceps militaris when temperature is 22 DEG C are best.
Embodiment 6
Difference cultivates container cordyceps space utilization rate and goes out careless comparative studies
1, test method
Cordyceps militaris strain is prepared by 1 method of embodiment, the pupa living of the autumn tussah after handling 7 days using 25 DEG C is material, examination
Points 2 groups are tested, every group takes 1000 tussah chrysalis at random, and inoculation position is at the 4th body segment of tussah chrysalis back, and inoculation bacterium solution dosage is equal
It is cultivated to be put into after 0.5ml, the 1st group of inoculation in vinyl disc (long 53cm × wide 40cm × high 10cm), every disk puts tussah chrysalis 200
Left and right is put into vial (diameter 10cm × high 10cm) after the 2nd group of inoculation and cultivates, and every bottle puts tussah chrysalis 6, and Cordyceps militaris is cultivated
Management method is identical with in example 1 1.3, after cultivating 45 days, investigates and analyses the space utilization rate of each test group cordyceps
With careless situation out.
2, test result
6 are shown in Table by the test result that above-mentioned test method is carried out
Table 6 is different to be cultivated container cordyceps space utilization rate and goes out careless comparison
| Cultivate container | Test pupa number/ | Tussah chrysalis number | Cultivate Cordyceps militaris number/m2 | Careless (%) out |
| Vinyl disc | 1000 | 200/disk | 940 | 91.7 |
| Vial | 1000 | 6/bottle | 600 | 89.4 |
It can see from 6 test result of table, be to cultivate container using vinyl disc (long 53cm × wide 40cm × high 10cm), often
Disk can cultivate cordyceps 200, and every square metre can cultivate Cordyceps militaris 940, and going out careless is 91.7/%；And it is sharp
It is to cultivate container with vial (diameter 10cm × high 10cm), every bottle can cultivate Cordyceps militaris 6, and every square metre can cultivate Cordyceps militaris
600, careless out is 89.4/%, and the space utilization rate of vinyl disc cultivating method improves 56.7% than vial cultivating method, and
It is careless out also to improve 2.3% than vial cultivating method.So greatly improving large-scale production using vinyl disc cultivating method
Space utilization rate, and technical operation is convenient, high production efficiency.
For any person skilled in the art, without departing from the scope of the technical proposal of the invention, all
Many possible changes and modifications are made to technical solution of the present invention using the technology contents of the disclosure above, or are revised as equivalent
The equivalent embodiment of variation.Therefore, anything that does not depart from the technical scheme of the invention, according to the technical essence of the invention to
Any simple modifications, equivalents, and modifications that upper embodiment is done should all still fall within the range of technical solution of the present invention protection
It is interior.
Claims (6)
1. a kind of high-efficient method for cultivating of cordyceps, it is characterised in that: including following operating procedure:
(1) the autumn tussah cocoon in duration of diapause of pupating of just cocooing is handled 6-8 days under the conditions of 19-28 DEG C, humidity 70-80%,
It is placed in spare after being stored 17-23 days in 0-4 DEG C；
(2) Cordyceps militaris slant strains are inoculated into Cordyceps militaris culture medium and are cultivated 3-5 days, inoculative proportion are as follows: take 0.2-
0.7cm2The 5-7 block slant strains of size, are inoculated into 200ml Cordyceps militaris culture medium；Aseptically cross 30-50 mesh
Filtrate is obtained after sieve, the filtrate of 2.5-7.5% switching by volume uses into fluid nutrient medium；
(3) after the tussah for taking step (1) to obtain pupa disinfection living, from its back 3-5 body segment, syringe needle is pierced to pupa cephalad direction
Enter about 1cm, the Cordyceps militaris strain 0.3ml-0.7ml of inoculation step (2) preparation puts down the tussah chrysalis needle mouth after inoculation upwards
It displays and is placed in culture plane, then covered with transparent membrane, in 18-25 DEG C of temperature, humidity 60-80%, intensity of illumination 600-
It is cultivated under conditions of 1000lux, daily 8-12 hours illumination, ventilation 2 times, can harvest by cultures in 45-50 days daily.
2. breeding method according to claim 1, it is characterised in that: the autumn toothed oak in duration of diapause in the step (1)
Silk cocoon cultivation temperature is 19-28 DEG C, and cultivation temperature is 25 DEG C；Incubation time is 7 days；Placing temperature is 2 DEG C, stores standby in 20 days
With.
3. breeding method according to claim 1, it is characterised in that: connect Cordyceps militaris slant strains in the step (2)
For kind to the step in Cordyceps militaris culture medium, the time of culture is 4 days.
4. breeding method according to claim 1, it is characterised in that: the injection at tussah pupa back living in the step (3)
Position is at the 4th body segment.
5. breeding method according to claim 1, it is characterised in that: the culture temperature of the tussah chrysalis in step (3) after inoculation
Degree is 19-22 DEG C.
6. breeding method according to claim 1, it is characterised in that: the illumination of the tussah chrysalis in step (3) after inoculation is strong
Degree is 800lux；Daily optimum illumination time is 12 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811034151.3A CN109105153B (en) | 2018-09-05 | 2018-09-05 | Efficient cultivation method of tussah cordyceps militaris |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811034151.3A CN109105153B (en) | 2018-09-05 | 2018-09-05 | Efficient cultivation method of tussah cordyceps militaris |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109105153A true CN109105153A (en) | 2019-01-01 |
| CN109105153B CN109105153B (en) | 2021-01-22 |
Family
ID=64858666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811034151.3A Active CN109105153B (en) | 2018-09-05 | 2018-09-05 | Efficient cultivation method of tussah cordyceps militaris |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109105153B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112391295A (en) * | 2020-11-17 | 2021-02-23 | 辽宁省海洋水产科学研究院 | Preparation method of wild cordyceps militaris strain |
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN114467625A (en) * | 2022-01-20 | 2022-05-13 | 辽宁省海洋水产科学研究院 | Method for cultivating high-quality tussah cordyceps militaris |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1288054A (en) * | 2000-11-03 | 2001-03-21 | 吉林东北虎药业股份有限公司 | Commercial prodn. mehtod for producing silkworm chrysalis Chinese caterpillar fungus |
| KR100610983B1 (en) * | 2004-07-26 | 2006-08-10 | 한국식품연구원 | Method for a cultivation of Cordyceps militaris by using Ginsaeng Culture medium |
| CN102342216A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Method by combining physical method and chemical method to improve quality of cordyceps militaris |
| CN102907256A (en) * | 2012-10-19 | 2013-02-06 | 湖北省农业科学院农产品加工与核农技术研究所 | Box-type cultivation method of tussah pupa cordyceps militaris |
| CN104823714A (en) * | 2015-05-04 | 2015-08-12 | 聂晶石 | Large-scale cultivation method for cordyceps militaris |
-
2018
- 2018-09-05 CN CN201811034151.3A patent/CN109105153B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1288054A (en) * | 2000-11-03 | 2001-03-21 | 吉林东北虎药业股份有限公司 | Commercial prodn. mehtod for producing silkworm chrysalis Chinese caterpillar fungus |
| KR100610983B1 (en) * | 2004-07-26 | 2006-08-10 | 한국식품연구원 | Method for a cultivation of Cordyceps militaris by using Ginsaeng Culture medium |
| CN102342216A (en) * | 2010-07-29 | 2012-02-08 | 于兴军 | Method by combining physical method and chemical method to improve quality of cordyceps militaris |
| CN102907256A (en) * | 2012-10-19 | 2013-02-06 | 湖北省农业科学院农产品加工与核农技术研究所 | Box-type cultivation method of tussah pupa cordyceps militaris |
| CN104823714A (en) * | 2015-05-04 | 2015-08-12 | 聂晶石 | Large-scale cultivation method for cordyceps militaris |
Non-Patent Citations (2)
| Title |
|---|
| 王乃红 等: "柞蚕蛹虫草的研究进展", 《中国蚕业》 * |
| 王贺祥 等: "《食用菌栽培手册》", 31 January 2015, 中国农业大学出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112391295A (en) * | 2020-11-17 | 2021-02-23 | 辽宁省海洋水产科学研究院 | Preparation method of wild cordyceps militaris strain |
| CN112391295B (en) * | 2020-11-17 | 2022-06-10 | 辽宁省海洋水产科学研究院 | Preparation method of wild cordyceps militaris strain |
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN112586513B (en) * | 2020-12-11 | 2021-11-19 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN114467625A (en) * | 2022-01-20 | 2022-05-13 | 辽宁省海洋水产科学研究院 | Method for cultivating high-quality tussah cordyceps militaris |
| CN114467625B (en) * | 2022-01-20 | 2024-02-02 | 辽宁省海洋水产科学研究院 | Cultivation method of high-quality tussah cordyceps militaris |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109105153B (en) | 2021-01-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103598010B (en) | Original ecological imitative wild cultivation method for inonotus sanghuang | |
| CN103804054B (en) | Phellinus culture medium is optimized and short segment wood cultivated method | |
| CN102440149B (en) | Method for breeding cordyceps militaris by utilizing silkworm larvae | |
| CN102786333B (en) | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same | |
| CN105210671B (en) | A kind of log glossy ganoderma breeding method | |
| CN102630481B (en) | Cultivation method for oospore oudemansiella mucida | |
| CN103270887B (en) | Silkworm chrysalis northern Chinese caterpillar Fungus industrial cultivation technique | |
| CN109105153A (en) | A kind of high-efficient method for cultivating of cordyceps | |
| CN106797804A (en) | A kind of method that silkworm using abnormal early stage cultivates Cordyceps militaris | |
| CN102763560A (en) | Cordyceps sobolifera fruiting body with cicada larva as host and culture method thereof | |
| CN105660191A (en) | Amauroderma rugosum sporocarp culture method | |
| CN104335820A (en) | Production method of gastrodia elata associated honey fungus strain | |
| CN106966774A (en) | A kind of method using cultivation flat mushroom of cracking rice | |
| CN103798058A (en) | Method for cultivating aseptic cordyceps militaris | |
| CN107691106A (en) | A kind of inoculation method of seafood mushroom culture medium | |
| CN107586725B (en) | Cordyceps liquid culture medium and method for culturing cordyceps by using same | |
| CN103493685A (en) | Method for cultivating phellinus igniarius by using wild living tree stumps | |
| CN105638244A (en) | Silkworm cordyceps militaris culturing method | |
| CN108684442A (en) | A kind of short bag intermediate cannula inoculation cured material bag-cultured of mushroom | |
| CN103636408A (en) | Factory-like production method of silkworm cordyceps | |
| CN111758484B (en) | Large-scale high-efficiency high-yield silkworm chrysalis cordyceps production and cultivation method | |
| CN107667776A (en) | A kind of Household edible mushroom planting method | |
| CN101569269B (en) | Production process for culturing Pleurotus eryngii by utilizing biological magnetized water | |
| CN105519353B (en) | A kind of preparation method of White mushroom strain | |
| Ragupathi et al. | Optimizing the growth conditions and adopting new methods growing oyster and milky mushrooms in same conditions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |