CN112586513A - Pure natural cordyceps militaris growth regulator - Google Patents

Abstract

The invention discloses a pure natural cordyceps militaris growth regulator which comprises the following components in percentage by mass: 3-5 parts of kelp powder, 3-5 parts of oak leaf extract, 1-3 parts of 5-year-old mature silkworm powder and 0.5-1 part of L-aspartic acid; the oak leaf extract is prepared by the following method: cleaning oak leaves, crushing the oak leaves by a dry method, adding ethanol with the mass 2-3 times of that of the oak leaf powder, standing for 2-3 h, adjusting the pH to 6.0 by using NaAc-HAc buffer solution, refluxing for 1-3 h at 78-80 ℃, collecting the refluxed extract, concentrating under reduced pressure, and performing spray drying to obtain an ethanol extract of the oak leaves; the 5-instar mature silkworm powder is obtained by that after 5-instar tussah silkworm larvae bred in autumn finish spinning and cocooning, silkworm bodies are gradually shortened and are still, the silkworm bodies enter a preparation stage before organ tissue decomposition and improvement, cocoon shells are cut off, the mature silkworms are taken out, and homogenization and spray drying are carried out.

Description

Pure natural cordyceps militaris growth regulator

Technical Field

The invention relates to the technical field of cultivation of cordyceps militaris, in particular to a pure natural cordyceps militaris growth regulator.

Background

Cordyceps militaris has biological activities of resisting tumor, resisting fungi, resisting inflammation, resisting fatigue, delaying aging, regulating immunity and the like, has good treatment and health care effects, and the culture technology of high-quality and high-yield fruiting bodies becomes a hotspot of research. At present, growth regulators applied to the aspect of increasing the yield of cordyceps militaris are mainly chemical preparations, hormones and the like, such as 2,4-D, triammonium citrate, urea and the like. The growth regulator can stimulate the growth of thallus, but because the growth regulator does not contain nutrient components, the contradiction between too fast growth and insufficient nutrient supply of the cordyceps militaris is easily caused, and meanwhile, chemical residues not only have adverse effects on the quality of the cordyceps militaris, but also pollute the environment.

The kelp powder is used as a natural marine alga, is rich in various nutritional ingredients and active substances which are lacked by terrestrial animals and plants, such as protein, algal polysaccharides, lipids, mineral elements, trace elements and active growth promoting factors, and is widely applied to aquatic feeds of sea cucumbers, fishes, shrimps, crabs and the like and livestock and poultry feeds of chickens, cows, pigs and the like. The oak belongs to the genus Quercus of the family Fagaceae, is a deciduous tree, and researches show that the stem and leaf of the oak contain 8.86 percent of crude protein, 0.42 percent of crude fat, 60.64 percent of crude cellulose and 5.43 percent of crude polysaccharide, have high nutritional value, and also contain some bioactive substances such as tannin, flavonoid and the like, and have the effects of resisting bacteria, inflammation and oxidation. The 5-year-old mature tussah larva bred in autumn is in the prophase of pupation and the preparation stage before decomposition of organ tissues, and is rich in nutrients such as protein, fat and the like in vivo, and various enzymes are in an active state, so that a series of antibacterial active substances such as antibacterial peptide, lysozyme and the like are generated, and the adult tussah larva has the effects of sterilization and disease resistance.

So far, no relevant report is available for preparing the cordyceps militaris growth regulator by mixing the kelp powder, the oak leaf extract, the 5-year-old silkworm powder and the L-aspartic acid in proportion.

Disclosure of Invention

The invention provides a pure natural cordyceps militaris growth regulator, and aims to solve the problems in the prior art.

The technical solution of the invention is as follows: a pure natural cordyceps militaris growth regulator comprises the following components in percentage by mass: 3-5 parts of kelp powder, 3-5 parts of oak leaf extract, 1-3 parts of 5-year-old mature silkworm powder and 0.5-1 part of L-aspartic acid;

the oak leaf extract is prepared by the following method: cleaning oak leaves, crushing the oak leaves by a dry method, adding ethanol with the mass 2-3 times of that of the oak leaf powder, standing for 2-3 h, adjusting the pH to 6.0 by using NaAc-HAc buffer solution, refluxing for 1-3 h at 78-80 ℃, collecting the refluxed extract, concentrating under reduced pressure, and performing spray drying to obtain an ethanol extract of the oak leaves;

the 5-instar mature silkworm powder is obtained by that after 5-instar tussah silkworm larvae bred in autumn finish spinning and cocooning, silkworm bodies are gradually shortened and are still, the silkworm bodies enter a preparation stage before organ tissue decomposition and improvement, cocoon shells are cut off, the mature silkworms are taken out, and homogenization and spray drying are carried out.

Compared with the prior art, the invention has the following advantages:

1. the cordyceps militaris cultivation medium is prepared from pure natural materials, is low in cost and free of chemical residues, is an environment-friendly growth regulator, and can effectively improve the cultivation yield and quality of cordyceps militaris;

2. the cordyceps militaris single-box medium is added into a cordyceps militaris culture medium according to the mass percentage of 1-5%, so that the carbon and nitrogen contents in the culture medium can be effectively improved, rich nutrition is provided for the growth of cordyceps militaris, the regulation and control capability of thalli can be enhanced, the growth of hyphae and the development and maturation of sporocarp can be promoted, a certain inhibition effect on mixed bacteria and the like can be realized, the disease is reduced, and the average yield of the cordyceps militaris single box can be improved by 17.84%;

the L-aspartic acid is used as a nutritional supplement, and is combined with other components to play a role of an intermediate in the organic synthesis of cordycepin, so that the cordycepin content in the cordyceps militaris can be increased by 10.47%.

Detailed Description

Example 1

A cordyceps militaris growth regulator is prepared by mixing the following components in percentage by mass: 3g of kelp powder, 3g of oak leaf extract, 1g of 5-year-old mature silkworm powder and 0.5g of L-aspartic acid;

kelp powder is produced by Dalianboshi Biotech Co., Ltd, and L-aspartic acid is purchased from Xin Feng Biochemical factory in Zibos shop in Shandong.

The oak leaf extract is prepared by the following method: cleaning oak leaves, crushing by a dry method, adding ethanol with the mass 2 times of that of the oak leaves powder, standing for 2 hours, adjusting the pH to 6.0 by using NaAc-HAc buffer solution, refluxing for 3 hours at 78 ℃, collecting the refluxed extract, concentrating under reduced pressure, and performing spray drying to obtain an ethanol extract of the oak leaves;

the 5-instar mature silkworm powder is obtained by that after 5-instar tussah silkworm larvae bred in autumn finish spinning and cocooning, silkworm bodies are gradually shortened and are still, the silkworm bodies enter a preparation stage before organ tissue decomposition and improvement, cocoon shells are cut off, the mature silkworms are taken out, and homogenization and spray drying are carried out.

Example 2

A cordyceps militaris growth regulator is prepared by mixing the following components in percentage by mass: 4g of kelp powder, 4g of oak leaf extract, 2g of 5-year-old mature silkworm powder and 0.5g of L-aspartic acid;

kelp powder is produced by Dalianboshi Biotech Co., Ltd, and L-aspartic acid is purchased from Xin Feng Biochemical factory in Zibos shop in Shandong.

The oak leaf extract is prepared by the following method: cleaning oak leaves, crushing by a dry method, adding ethanol with the mass 2.5 times of that of the oak leaf powder, standing for 2.5 hours, adjusting the pH to 6.0 by using NaAc-HAc buffer solution, refluxing for 2 hours at 78 ℃, collecting a refluxing extract, concentrating under reduced pressure, and performing spray drying to obtain an ethanol extract of the oak leaves;

the 5-instar mature silkworm powder is the 5-instar tussah silkworm larvae bred in autumn, after spinning and cocooning are finished, silkworm bodies are gradually shortened and kept still, the silkworm bodies enter a preparation stage before organ tissue decomposition and improvement, the relative experience time of the period is long, the resistance of the silkworm bodies to the external mechanical and physical influence is strong, the period is the optimal period of transportation, cold storage and cocoon splitting, the mature silkworms are taken out after cocoon shells are cut, and the 5-instar mature silkworm powder is obtained through homogenization, spray and drying.

Example 3

A cordyceps militaris growth regulator is prepared by mixing the following components in percentage by mass: 5g of kelp powder, 5g of oak leaf extract, 3g of 5-year-old mature silkworm powder and 1g of L-aspartic acid;

kelp powder is produced by Dalianboshi Biotech Co., Ltd, and L-aspartic acid is purchased from Xin Feng Biochemical factory in Zibos shop in Shandong.

The oak leaf extract is prepared by the following method: cleaning oak leaves, crushing by a dry method, adding ethanol with the mass of 3 times of that of the oak leaves powder, standing for 3 hours, adjusting the pH to 6.0 by using NaAc-HAc buffer solution, refluxing for 1 hour at 80 ℃, collecting the refluxed extract, concentrating under reduced pressure, and performing spray drying to obtain an ethanol extract of the oak leaves;

the 5-instar mature silkworm powder is the 5-instar tussah silkworm larvae bred in autumn, after spinning and cocooning are finished, silkworm bodies are gradually shortened and kept still, the silkworm bodies enter a preparation stage before organ tissue decomposition and improvement, the relative experience time of the period is long, the resistance of the silkworm bodies to the external mechanical and physical influence is strong, the period is the optimal period of transportation, cold storage and cocoon splitting, the mature silkworms are taken out after cocoon shells are cut, and the 5-instar mature silkworm powder is obtained through homogenization, spray and drying.

Experiment:

in order to investigate the influence of the invention on the growth characteristics of cordyceps militaris, 8 treatment groups are designed, wherein a wheat culture medium of the treatment group 1 is a control group, additives with the mass of 3% are added into the wheat culture medium of other groups, and the additives from the treatment group 2 to the treatment group 8 are respectively kelp powder used in the embodiment of the invention, the embodiment 2 and the embodiment 3, oak leaf extract used in the embodiment of the invention, 5-year-old silkworm powder used in the embodiment of the invention and L-aspartic acid used in the embodiment of the invention.

Preparing 90 boxes of culture medium for each treatment group, wherein each box is 500g (specification is 30 multiplied by 30 cm), 3 times of repetition are respectively set, cordyceps militaris strains are inoculated and then timely transferred into a strain growing chamber, the temperature is controlled to be constant temperature culture at 17-19 ℃, the relative humidity is controlled to be 65% -70%, after 7-14 days, illumination is carried out after the mycelia are fully grown, the illumination intensity is 150-250 Lux, no less than 2 hours every day, the culture temperature is 18-20 ℃, 2-3 days are changed from white to orange, the sealing film is perforated and ventilated, a germination accelerating stage is carried out, the temperature is kept at 18-20 ℃ in the daytime and 15-17 ℃ in the evening, when a certain amount of orange bulges appear on the surface of the culture medium, a fruiting body culture stage is carried out, and the temperature of the culture chamber is kept between 15. And irradiating the fruiting bodies with light of a fluorescent lamp for hours during the growth period, ventilating the culture room for 1-2 times every day, keeping the relative humidity of air at 80-90%, and harvesting, airing and storing the fruiting bodies after the fruiting bodies are mature in 50-60 days generally. The growth characteristics and the morbidity of cordyceps militaris are investigated after inoculation and during the harvest period, the sporophores are weighed, and the cordycepin content is determined, and the results are shown in table 1.

Table 1 Effect of the growth regulator of the invention on Cordyceps militaris growth and cordycepin content

As can be seen from Table 1: in the invention, 4 materials generate a synergistic effect mutually, the yield and the quality of the cordyceps militaris are obviously improved, the yield of a single box of the cordyceps militaris can be improved by 22.76%, the cordycepin content in the cordyceps militaris is increased to 221mg/100g, the morbidity is reduced, and the effect of any one component is obviously lower than that of the cordyceps militaris when the component is used alone.

Claims (1)

1. A pure natural cordyceps militaris growth regulator is characterized by comprising the following components in percentage by mass: 3-5 parts of kelp powder, 3-5 parts of oak leaf extract, 1-3 parts of 5-year-old mature silkworm powder and 0.5-1 part of L-aspartic acid;

the oak leaf extract is prepared by the following method: cleaning oak leaves, crushing the oak leaves by a dry method, adding ethanol with the mass 2-3 times of that of the oak leaf powder, standing for 2-3 h, adjusting the pH to 6.0 by using NaAc-HAc buffer solution, refluxing for 1-3 h at 78-80 ℃, collecting the refluxed extract, concentrating under reduced pressure, and performing spray drying to obtain an ethanol extract of the oak leaves;

the 5-instar mature silkworm powder is obtained by that after 5-instar tussah silkworm larvae bred in autumn finish spinning and cocooning, silkworm bodies are gradually shortened and are still, the silkworm bodies enter a preparation stage before organ tissue decomposition and improvement, cocoon shells are cut off, the mature silkworms are taken out, and homogenization and spray drying are carried out.