CN105146053A - Method for production of high protein feed from edible fungus chaff - Google Patents
Method for production of high protein feed from edible fungus chaff Download PDFInfo
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- CN105146053A CN105146053A CN201510386419.XA CN201510386419A CN105146053A CN 105146053 A CN105146053 A CN 105146053A CN 201510386419 A CN201510386419 A CN 201510386419A CN 105146053 A CN105146053 A CN 105146053A
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- high protein
- protein feed
- edible fungus
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- bacterium
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for production of high protein feed from edible fungus chaff. The method specifically includes: breaking a fungus stick to obtain fungus chaff, adding an enzyme production induction liquid, then conducting culture under suitable conditions, eluting the fermentation system to obtain ligninase and efficiently transformed fungus chaff, then adding Tenebrio molitor, fly larvae, earthworms, locusts, silkworm chrysalis and other insect larvae into the remaining material to conduct culture, thus obtaining a high protein feed culture system. Compared with other production processes producing feed from biomass, the method can acquire ligninase, and realizes absorption and utilization of fungus chaff residue by the insect larvae so as to obtain the high protein feed. And pollution generated in the edible fungus production process is reduced.
Description
Technical field
Utilize edible fungus bran to produce a method for high protein feed, be specifically related to utilize edible fungus bran to produce the method for high protein feed.Belong to technical field of bioengineering.
Background technology
Edible fungus bran to refer in Edible Fungi process after fruiting or the synthesis comprising edible mushroom and biomass material of not yet fruiting, is rich in mycoprotein, is therefore called as bacterium chaff albumen.According to surveying and determination, in bacterium chaff, protein content reaches 12% ~ 21%.But because edible mushroom chaff is rich in cellulose and lignin such as husk, straw, wood chip etc. grow out multiple, although bacterium chaff contains a large amount of bacterium chaff albumen, but it can not directly add in animal diet as feed, because the crude fibre of original edible fungi residues is very high, exceed the standard that conventional animal is digested and assimilated, and can not full feeding condition in smell and palatability, can't preserve for a long time.Therefore at present edible fungi residues is all carry out burning or landfill after being used to dry, and may cause the breeding of pathogen in the process of drying, and burns and also can cause air pollution.Not only do not play the economic worth of bacterium chaff fully, also can bring pollution on the contrary.
Insect is most species and the huge biology of biomass on the earth, biomass exceedes 10 times of other all animals (comprising the mankind) biomass, its efficiency of food conversion is high, reproduction speed is fast, the growth cycle of most insects is all shorter, can several generations have been bred in 1 year, have even more than 50 generations.There are some researches show at present: insect as protein feeds on part breed variety, can Peru Fish Dietary completely, few, the easy absorption of the high and low fat of insect protein content, low cholesterol, fleshy fiber, that trace element enriches, is of high nutritive value and breeds speed is fast, and feeding facility is simply with low cost, so there is scientist to foretell 21 century, insect will be the important sources of animal protein.
Summary of the invention:
The object of the present invention is to provide a kind of method utilizing edible fungus bran to produce high protein feed, the bacterium rod containing hypha of edible fungus is utilized to be raw material, add wherein and produce enzyme induction liquid promotion edible mushroom making full use of biomass material, and then obtain lignoenzyme and the bacterium chaff residue containing more reduced sugar.By insect larvae to the recuding sugars in bacterium chaff residue and amino acids sorption enhanced and then obtain high protein feed.The bacterium rod in agricultural production is made to obtain high-valued recycling.
Bacterium rod refers to cotton seed hulls, wood chip, straw, corncob, bagasse and multiple kinds of crops stalk, industrial waste for primary raw material, is used for the bar-shaped solid mixt producing fruit body of edible fungi or fructification of gathering is later remaining after inoculation hypha of edible fungus cultivates a period of time.When hypha of edible fungus grows in the medium, need to produce a large amount of lignoenzyme to decompose lignocellulosic in matrix to obtain nutrition.Therefore, results or not yet gather in the crops fructification bacterium rod in containing a large amount of hypha of edible fungus.The concrete composition of bacterium rod can not as the restriction to high-protein feeding preparation method for material of the present invention.
Industrial waste is mainly selected from vinegar grain, vinasse, paper mill effluent.
Edible mushroom is the artificial mushroom cultivated or can be under wild environment biology and eat.Lignoenzyme can be secreted to decompose lignocellulosic in matrix to obtain nutrition in its growth course.Described edible mushroom is mainly selected from mushroom (Agaricuscampestris), mushroom (Lentinulaedodes), agrocybe (Agrocybeaegerita), Hericium erinaceus (Hericiumerinaceum), phoenix-tail mushroom (Pleurotussajor-caju), agaricus bisporus (Agaricusbisporus), auricularia auriculajudae (Auriculariaauricula), Cordyceps sinensis (Cordycepssinensis), glossy ganoderma (Ganodermalucidum), pycnoporus samguineus (Pycnoporussanguineus), gold ear (Tremellaaurantialba), grey mushroom (abalonelike) (Pleurotusabalonus), flat mushroom (Pleurotusostreatus), asparagus (Flammulinavelutipes), pleurotus eryngii (Pleurotuseryngii), elegant precious mushroom (Pleurotusgeesteranus), coprinus comatus (Coprinuscomatus), Pleurotus nebrodensis (Pleurotusnebrodensis), lung shape picks up the ears (Pleurotuspulmonarius), Agricus blazei (Agaricusblazei) etc.
Technical scheme of the present invention:
The invention provides a kind of method utilizing edible fungus bran to produce high protein feed, specifically comprise the steps: that the break process of bacterium rod obtains bacterium chaff; Add and produce enzyme induction liquid; Cultivate under suitable condition; Fermentation system wash-out obtains lignoenzyme and bacterium chaff residue; Add after insect larvae is cultivated and obtain feed.
1. broken bacterium rod obtains bacterium chaff
After the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff.
2. add induction and produce enzyme liquid
The inducer that enzyme liquid contains lignoenzyme is produced in induction used, and other substances provide nitrogen element and trace element for hypha of edible fungus, can also adjust carbon-nitrogen ratio (C/N) and the pH value of system in addition, stimulates mycelia diauxic growth.Induction is produced enzyme liquid and is obtained by the prolonged and repeated test in this laboratory.
A kind of concrete embodiment, the usual addition that enzyme liquid is produced in every 10kg bacterium chaff induction is 10-50L, 10-30L can be selected further, the diauxic growth that more effectively can stimulate hypha of edible fungus in bacterium chaff when 12,13,15,16,17,19,20,23,25 or 28L is selected, the high expression of induction lignoenzyme and secretion when concrete.
3. cultivate under suitable condition
Suitable extraneous condition of culture can change the physiological status of hypha of edible fungus, better expression and secretion lignoenzyme, and then biomass material is fully degraded.
A concrete embodiment, cultivation temperature is generally 17-36 DEG C, can be chosen as 22-30 DEG C further, or is specifically chosen as 22,24,25,27,28,30 DEG C.The time of cultivating under above temperature conditions is 24-168 hour, or selects 24,72,96,120 or 144 hours further, can obtain that lignoenzyme content is higher, lignocellulosic transforms bacterium chaff more thoroughly.
4. the wash-out of fermentation system
What obtain to step 3 contains the distilled water immersion adding 1-3 times of volume in the fermentation maturation system of a large amount of lignoenzyme, and vibration is filtered.Obtain lignoenzyme crude enzyme liquid and bacterium chaff residue.The lignoenzyme total amount obtained when distilled water volume is very few will be very few, when distilled water volume is excessive, the material such as carbohydrate, amino acid in bacterium chaff residue will be made to reduce, be unfavorable for the growth of insect larvae, reduces the feed nutritive value finally obtained.
5. add insect larvae to cultivate
Obtain to step 4 larva that bacterium chaff residue adds the insects such as yellow meal worm, fly maggot, earthworm, locust and silkworm chrysalis to cultivate.According to caste determination optimum condition and incubation time.
Beneficial effect of the present invention:
The invention provides a kind of method utilizing edible fungus stick to produce high protein feed.Be a kind of new way of bacterium chaff higher value application, in production process, can also lignoenzyme be obtained.Both can accomplish twice laid environmental protect, the production cost of lignoenzyme can be reduced again, there is good application prospect.It is a kind of brand-new feed production method.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention is described further.Following examples are only several specific embodiment of the present invention, but design concept of the present invention is not limited thereto, and all changes utilizing this design the present invention to be carried out to unsubstantiality, all should belong to the behavior of invading scope.
Embodiment 1
Get 10kg pleurotus eryngii bacterium rod, fragmentation adds the product enzyme induction liquid of 15L after obtaining bacterium chaff, and cultivate 3 days at 23 DEG C, the system obtained adds 40L distilled water immersion, and vibration is filtered and obtained crude enzyme liquid and bacterium chaff residue.In residual bacterium chaff, continue the Yellow meal worm larva adding 500g, at 30 DEG C, relative humidity 75%, is cultured to each instar larvae under intensity of illumination 400lx.Post-drying of sieving is for subsequent use, obtains high protein feed.
Embodiment 2
Get 10kg flat mushroom bacterium rod, fragmentation adds the product enzyme induction liquid of 20L after obtaining bacterium chaff, and cultivate 6 days at 25 DEG C, the system obtained adds 50L distilled water immersion, and vibration is filtered and obtained crude enzyme liquid and bacterium chaff residue.Continue to add 800g earthworm larva in residual bacterium chaff, at 23 DEG C, relative humidity 85%, cultivates under intensity of illumination 200lx.Post-drying of sieving is for subsequent use, obtains high protein feed.
Claims (7)
1. the method utilizing edible fungus bran to produce high protein feed, be characterized in that the bacterium chaff to obtain after edible fungus stick fragmentation is raw material, add product enzyme inducer, obtain high protein feed to adding after insect larvae is cultivated after fermentation maturation system wash-out, the method comprises the following steps again:
A) by after the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff;
B) in obtained bacterium chaff, add product enzyme induction liquid according to the ratio of solid-liquid mass volume ratio 1: 1-4, after mixing, obtain fermentation system;
C) fermentation system is cultivated 24-168 hour under 17-36 DEG C of condition, carry out fermenting and producing lignocellulolyticenzymes;
The distilled water wash-out of the fermentation maturation system 1-3 times of volume d) obtained obtains crude enzyme liquid;
E) after wash-out, remaining solid matter adds insect larvae and obtains high protein feed cultivating system;
F) cultivation is carried out to high protein feed cultivating system and obtain high protein feed.
2. a kind of method utilizing edible fungus bran to produce high protein feed according to claim 1, is characterized in that described product enzyme induction formula of liquid is: in mass, urea is 0.1 ~ 5%, ammonium nitrate is 0.2 ~ 6%.
3. a kind of method utilizing edible fungus bran to produce high protein feed according to claim 1, is characterized in that every 10kg bacterium rod needs the product enzyme induction liquid of 10L-40L.
4. a kind of method utilizing edible fungus bran to produce high protein feed according to claim 1, is characterized in that cultivation temperature is 17-36 DEG C.
5. a kind of method utilizing edible fungus bran to produce high protein feed according to claim 1, is characterized in that incubation time is 24-168 hour.
6. a kind of method utilizing edible fungus bran to produce high protein feed according to claim 1, is characterized in that the mass volume ratio of fermentation system and each wash-out water is 1: 1-3.
7. a kind of method utilizing edible fungus bran to produce high protein feed according to claim 1, is characterized in that the larva adding the insects such as yellow meal worm, fly maggot, earthworm, locust and silkworm chrysalis after wash-out in remaining material.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171524A (en) * | 2016-07-26 | 2016-12-07 | 李渝方 | The method that Rosa setate X R. rugosa slag for comprehensive utilizes |
| CN106974143A (en) * | 2017-05-16 | 2017-07-25 | 曹亚军 | It is a kind of to be used to raise mixed fodder of yellow meal worm and preparation method thereof |
| CN109874601A (en) * | 2019-04-02 | 2019-06-14 | 苏冰 | The method that edible fungi residue is converted to culture medium of edible fungus using earthworm |
| CN112042831A (en) * | 2020-09-28 | 2020-12-08 | 井冈山大学 | Preparation method of protein feed by mixed fermentation of fish leftovers and soybean meal |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
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2015
- 2015-07-01 CN CN201510386419.XA patent/CN105146053A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171524A (en) * | 2016-07-26 | 2016-12-07 | 李渝方 | The method that Rosa setate X R. rugosa slag for comprehensive utilizes |
| CN106974143A (en) * | 2017-05-16 | 2017-07-25 | 曹亚军 | It is a kind of to be used to raise mixed fodder of yellow meal worm and preparation method thereof |
| CN109874601A (en) * | 2019-04-02 | 2019-06-14 | 苏冰 | The method that edible fungi residue is converted to culture medium of edible fungus using earthworm |
| CN112042831A (en) * | 2020-09-28 | 2020-12-08 | 井冈山大学 | Preparation method of protein feed by mixed fermentation of fish leftovers and soybean meal |
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Application publication date: 20151216 |