CN105039467A - Cordyceps militaris liquid culture medium and culture method of cordyceps militaris - Google Patents
Cordyceps militaris liquid culture medium and culture method of cordyceps militaris Download PDFInfo
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- CN105039467A CN105039467A CN201510356495.6A CN201510356495A CN105039467A CN 105039467 A CN105039467 A CN 105039467A CN 201510356495 A CN201510356495 A CN 201510356495A CN 105039467 A CN105039467 A CN 105039467A
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Abstract
The invention relates to a cordyceps militaris liquid culture medium which comprises the following components: 1-3 g/L of KH2PO4, 1-3 g/L of MgSO4, 5-20 g/L of a nitrogen source, 200-500 g/L of potatoes, 8-15 g/L of D-fructose, 20-50 g/L of agar, 10-25 g/L of basic amino acid, 8-20 g/L of acidic amino acid, and 30-50 g/L of silkworm chrysalis meal. The liquid culture medium provided by the invention uses D-fructose as a carbon source; basic amino acid and acidic amino acid are added to the culture medium; the yield of the cordyceps militaris cultured by using the liquid culture medium is remarkably improved, and the production of cordycepin in the cordyceps militaris is also improved; the production cycle is short; the follow-up extraction and separation of cordycepin are convenient; the cordyceps militaris liquid culture medium is suitable for industrial production.
Description
Technical field
The invention belongs to biological technical field, be specifically related to the cultural method of a kind of Cordyceps militaris substratum and Cordyceps militaris (L.) Link..
Background technology
Cordyceps militaris (L.) Link. (Cordycepsmilitaris) has another name called Cordyceps militaris (L.) Link., be under the jurisdiction of Ascomycota Ascomycota excrement shell Gammaproteobacteria Sordariomycetes, Hypocreales Hypocreales, Chinese caterpillar fungus section Cordycipitaceae, Cordyceps Cordyceps is the type species of Cordyceps.On the pupal cell of the lepidopterous insects of wild cordyceps militaris main parasitic in softwood forest, broad-leaf forest or mixed forest surface soil layer, be also found parasitize the insect such as Coleoptera and Diptera pupa, on adult or larva body.Cordyceps militaris (L.) Link. contains various bioactivators, comprise the nucleosides material such as cordycepin, adenosine, also comprise Cordyceps polysaccharide, SOD(superoxide-dismutase) and amino acid etc., and wherein topmost activeconstituents is cordycepin, research shows that cordycepin has the multiple pharmacologically actives such as immunity moderation, antitumor, antimycotic, leukemia, reducing blood-fat, therefore, the market requirement is increasing.
The content of cordycepin in Cordyceps militaris (L.) Link. is extremely low, although cordycepin is by chemosynthesis, but synthesis technique is complicated, product recoveries is low, production cost is high, therefore, current most study still how obtain cordycepin by Cordyceps militaris (L.) Link. fermentation, but how to optimize the culture condition of Cordyceps militaris (L.) Link., the content of the growth of raising Cordyceps militaris (L.) Link. and wherein cordycepin is the problem received much concern always.The invention provides a kind of liquid deep drainpipe method of Cordyceps militaris (L.) Link., Cordyceps militaris (L.) Link. can be produced fast, also can improve the output of its cordycepin simultaneously.
Summary of the invention
The object of the invention is to solve the above-mentioned problems in the prior art, a kind of Cordyceps militaris substratum is provided, easy and simple to handle, and cordycepin output is high.
Technical scheme of the present invention is as follows:
A kind of Cordyceps militaris substratum, described substratum comprises and being made up of each component of following content:
KH2PO41-3g/L，
MgSO41-3g/L，
Nitrogenous source 5-20g/L,
Potato 200-500g/L,
D-Fructose 8-15g/L,
Agar 20-50g/L,
Basic aminoacids 10-25g/L,
Acidic amino acid 8-20g/L,
Dried silkworm chrysalis meal 30-50g/L.
Preferably, described nitrogenous source be selected from peptone, extractum carnis or yeast extract paste one or more.
Preferably, the concentration of described peptone is 7.5-15g/L.
Preferably, the concentration of described D-Fructose is 10-12g/L.
Preferably, described basic aminoacids be selected from arginine, Methionin or Histidine one or more.
Preferably, described acidic amino acid be selected from L-glutamic acid or aspartic acid one or more.
Preferably, the PH of described substratum is 5-8.
Adopt Cordyceps militaris substratum as above to carry out the method for Cordyceps militaris (L.) Link. cultivation, comprise the following steps:
(1) shake-flask culture: undertaken joining foster basigamy system by the basal medium formulation of KH2PO41-3g/L, MgSO41-3g/L, nitrogenous source 5-20g/L, potato 200-500g/L, D-Fructose 8-15g/L, agar 20-50g/L, basic aminoacids 10-25g/L, acidic amino acid 8-20g/L, dried silkworm chrysalis meal 30-50g/L, high pressure steam sterilization 10-30min, at normal temperatures after cooling, be loaded in the wide-necked bottle of 500ml, after inoculation Cordyceps militaris spawn, at unglazed photograph and culture temperature is at 25-28 DEG C, obtain fermented liquid with 150-200r/min shake-flask culture 7-10d;
(2) cordycepin content measures: the fermented liquid after the cultivation in step (1) terminates, and with the centrifugal 10-30min of 3000-5000r/min, collects supernatant liquor, thin up, through 0.45um membrane filtration, gets filtrate and adopts HPLC method to measure the content of cordycepin.
Preferably, the Cordyceps militaris spawn of the inoculation described in step (1) and the volume ratio of substratum are 1:(30-50).
Preferably, in step (1) when cultivation 4d, add VITAMIN B4 1-3g/L and glycine 16-40g/L.
Compared with prior art, beneficial effect is embodied in the present invention:
1. in Cordyceps militaris (L.) Link. process of growth, the cordycepin of 98% is secreted in substratum, and from nutrient solution, extract cordycepin, and not only extract conveniently, and with short production cycle, adopt the liquid nutrient medium in the present invention to carry out liquid deep drainpipe to Cordyceps militaris (L.) Link., the content of Cordyceps militaris (L.) Link. cordycepin can be significantly improved.
2. the present invention adopts D-Fructose as the carbon source in substratum, and it can significantly improve the content of cordycepin, apparently higher than other carbon sources (glucose, starch, lactose and Mierocrystalline cellulose etc.); Peptone, extractum carnis or yeast extract paste three kinds of organic nitrogen sources as the nitrogenous source of substratum, also can be significantly improved the output of cordycepin by the present invention.
3. amino acid is the basal component forming protein, and it is in close relations with the vital movement of fungi, foreign scholar studies display, when Cordyceps militaris (L.) Link. is cultivated, when adding the amino acid such as a certain amount of glycine, aspartic acid, the cordycepin content in Cordyceps militaris (L.) Link. can be made obviously to increase, the present invention selects three kinds of the strongest basic aminoacidss of Cordyceps militaris (L.) Link. cordycepin growth-promoting effect and two kinds of acidic amino acids, it is made an addition in basic medium with certain proportion, the output improving Cordyceps militaris (L.) Link. cordycepin can be shown.
4. VITAMIN B4 and glycine are the precursor substances of cordycepin synthesis, the present invention, when fermentation culture 4d, with the addition of this two kinds of precursor substances in the medium, and Cordyceps militaris (L.) Link. directly can utilize this two kinds of material synthesis cordycepins, therefore, the output of Cordyceps militaris (L.) Link. cordycepin can also significantly be increased.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail:
Embodiment 1
A kind of Cordyceps militaris substratum, described substratum comprises and being made up of each component of following content:
KH2PO41g/L，
MgSO41g/L，
Peptone 5g/L,
Potato 200g/L,
D-Fructose 8g/L,
Agar 20g/L,
Arginine 10g/L,
L-glutamic acid 8g/L,
Dried silkworm chrysalis meal 30g/L.
The PH of described substratum is 5.
Adopt Cordyceps militaris substratum as above to carry out the method for Cordyceps militaris (L.) Link. cultivation, comprise the following steps:
(1) shake-flask culture: undertaken joining foster basigamy system by the basal medium formulation of KH2PO41g/L, MgSO41g/L, peptone 5g/L, potato 200g/L, D-Fructose 8g/L, agar 20g/L, arginine 10g/L, L-glutamic acid 8g/L, dried silkworm chrysalis meal 30g/L, high pressure steam sterilization 10min, at normal temperatures after cooling, be loaded in the wide-necked bottle of 500ml, after the amount being 1:30 by the volume ratio of Cordyceps militaris spawn and substratum is inoculated, at unglazed photograph and culture temperature is at 25 DEG C, obtain fermented liquid with 150r/min shake-flask culture 7d;
(2) cordycepin content measures: the fermented liquid after the cultivation in step (1) terminates, and with the centrifugal 10min of 3000r/min, collects supernatant liquor, thin up, through 0.45um membrane filtration, gets filtrate and adopts HPLC method to measure the content of cordycepin.
In step (1) when cultivation 4d, add VITAMIN B4 1g/L and glycine 16g/L.
Embodiment 2
A kind of Cordyceps militaris substratum, described substratum comprises and being made up of each component of following content:
KH2PO43g/L，
MgSO43g/L，
Extractum carnis 20g/L,
Potato 500g/L,
D-Fructose 15g/L,
Agar 50g/L,
Methionin 25g/L,
Aspartic acid 20g/L,
Dried silkworm chrysalis meal 50g/L.
The PH of described substratum is 8.
Adopt Cordyceps militaris substratum as above to carry out the method for Cordyceps militaris (L.) Link. cultivation, comprise the following steps:
(1) shake-flask culture: undertaken joining foster basigamy system by the basal medium formulation of KH2PO43g/L, MgSO43g/L, extractum carnis 20g/L, potato 500g/L, D-Fructose 15g/L, agar 50g/L, Methionin 25g/L, aspartic acid 20g/L, dried silkworm chrysalis meal 50g/L, high pressure steam sterilization 30min, at normal temperatures after cooling, be loaded in the wide-necked bottle of 500ml, be after 1:50 inoculates Cordyceps militaris spawn by the volume ratio of Cordyceps militaris spawn and substratum, at unglazed photograph and culture temperature is at 28 DEG C, obtain fermented liquid with 200r/min shake-flask culture 10d;
(2) cordycepin content measures: the fermented liquid after the cultivation in step (1) terminates, and with the centrifugal 30min of 5000r/min, collects supernatant liquor, thin up, through 0.45um membrane filtration, gets filtrate and adopts HPLC method to measure the content of cordycepin.
In step (1) when cultivation 4d, add VITAMIN B4 3g/L and glycine 40g/L.
Embodiment 3
A kind of Cordyceps militaris substratum, described substratum comprises and being made up of each component of following content:
KH2PO42g/L，
MgSO42g/L，
Peptone 15g/L,
Potato 350g/L,
D-Fructose 12g/L,
Agar 35g/L,
Histidine 18g/L,
L-glutamic acid 15g/L,
Dried silkworm chrysalis meal 45g/L.
The PH of described substratum is 7.
Adopt Cordyceps militaris substratum as above to carry out the method for Cordyceps militaris (L.) Link. cultivation, comprise the following steps:
(1) shake-flask culture: undertaken joining foster basigamy system by the basal medium formulation of KH2PO42g/L, MgSO42g/L, peptone 15g/L, potato 350g/L, D-Fructose 12g/L, agar 35g/L, Histidine 18g/L, L-glutamic acid 15g/L, dried silkworm chrysalis meal 45g/L, high pressure steam sterilization 20min, at normal temperatures after cooling, be loaded in the wide-necked bottle of 500ml, be after 1:40 inoculates Cordyceps militaris spawn by the volume ratio of Cordyceps militaris spawn and substratum, at unglazed photograph and culture temperature is at 26 DEG C, obtain fermented liquid with 180r/min shake-flask culture 8d;
(2) cordycepin content measures: the fermented liquid after the cultivation in step (1) terminates, and with the centrifugal 20min of 4000r/min, collects supernatant liquor, thin up, through 0.45um membrane filtration, gets filtrate and adopts HPLC method to measure the content of cordycepin.
In step (1) when cultivation 4d, add VITAMIN B4 2g/L and glycine 25g/L.
Above-described embodiment is only the preferred embodiment of the present invention, and should not be construed as limitation of the invention, and those skilled in the art, according to announcement of the present invention, do not depart from improvement that scope makes and amendment all should within protection scope of the present invention.
Claims (10)
1. a Cordyceps militaris substratum, is characterized in that, described substratum comprises and being made up of each component of following content:
KH2PO41-3g/L，
MgSO41-3g/L，
Nitrogenous source 5-20g/L,
Potato 200-500g/L,
D-Fructose 8-15g/L,
Agar 20-50g/L,
Basic aminoacids 10-25g/L,
Acidic amino acid 8-20g/L,
Dried silkworm chrysalis meal 30-50g/L.
2. described a kind of Cordyceps militaris substratum according to claim 1, is characterized in that, described nitrogenous source be selected from peptone, extractum carnis or yeast extract paste one or more.
3. described a kind of Cordyceps militaris substratum according to claim 2, it is characterized in that, the concentration of described peptone is 7.5-15g/L.
4. described a kind of Cordyceps militaris substratum according to claim 1, it is characterized in that, the concentration of described D-Fructose is 10-12g/L.
5. described a kind of Cordyceps militaris substratum according to claim 1, is characterized in that, described basic aminoacids be selected from arginine, Methionin or Histidine one or more.
6. described a kind of Cordyceps militaris substratum according to claim 1, is characterized in that, described acidic amino acid be selected from L-glutamic acid or aspartic acid one or more.
7. described a kind of Cordyceps militaris substratum according to claim 1, it is characterized in that, the PH of described substratum is 5-8.
8. adopt as arbitrary in claim 1 ~ 7 as described in Cordyceps militaris substratum carry out the method for Cordyceps militaris (L.) Link. cultivation, it is characterized in that, comprise the following steps:
(1) shake-flask culture: undertaken joining foster basigamy system by the basal medium formulation of KH2PO41-3g/L, MgSO41-3g/L, nitrogenous source 5-20g/L, potato 200-500g/L, D-Fructose 8-15g/L, agar 20-50g/L, basic aminoacids 10-25g/L, acidic amino acid 8-20g/L, dried silkworm chrysalis meal 30-50g/L, high pressure steam sterilization 10-30min, at normal temperatures after cooling, be loaded in the wide-necked bottle of 500ml, after inoculation Cordyceps militaris spawn, at unglazed photograph and culture temperature is at 25-28 DEG C, obtain fermented liquid with 150-200r/min shake-flask culture 7-10d;
(2) cordycepin content measures: the fermented liquid after the cultivation in step (1) terminates, and with the centrifugal 10-30min of 3000-5000r/min, collects supernatant liquor, thin up, through 0.45um membrane filtration, gets filtrate and adopts HPLC method to measure the content of cordycepin.
9. the method for Cordyceps militaris (L.) Link. cultivation according to claim 8, it is characterized in that, the Cordyceps militaris spawn of the inoculation described in step (1) and the volume ratio of substratum are 1:(30-50).
10. the method for Cordyceps militaris (L.) Link. cultivation according to claim 8, is characterized in that, in step (1) when cultivation 4d, add the fast 1-3g/L of gland and glycine 16-40g/L.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441336A (en) * | 2015-12-23 | 2016-03-30 | 上海市农业科学院 | Method for effectively preventing degradation of filamentous fungi and restoring spore production |
| CN106381253A (en) * | 2016-08-31 | 2017-02-08 | 安康市蚕桑产业发展中心 | Process for nutritional enhancing production of cordyceps militaris yellow rice wine by cordyceps militaris rice culture material |
| CN106399124A (en) * | 2016-08-31 | 2017-02-15 | 徐春生 | Solid medium of cordyceps sinensis mycelia |
| CN106577925A (en) * | 2016-12-23 | 2017-04-26 | 山东建筑大学 | Cordyceps militaris pizza and preparation method of the same |
| CN106605684A (en) * | 2016-12-23 | 2017-05-03 | 山东建筑大学 | Red yeast rice pizza and preparation method thereof |
| CN106754404A (en) * | 2016-11-21 | 2017-05-31 | 王军 | A kind of Chinese caterpillar fungus culture medium for improving cordycepin content |
| CN106819007A (en) * | 2016-12-23 | 2017-06-13 | 山东建筑大学 | A kind of probiotics Pizza and preparation method thereof |
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN113005163A (en) * | 2021-03-25 | 2021-06-22 | 泸州品创科技有限公司 | Method for producing cordycepin by continuously culturing cordyceps militaris |
| CN113151007A (en) * | 2021-03-30 | 2021-07-23 | 浙江工业大学 | Liquid fermentation medium for increasing cordycepin content in cordyceps militaris |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935979A (en) * | 2006-11-01 | 2007-03-28 | 周永祥 | Coragyceps militaris bacterium culture method |
| CN103396193A (en) * | 2013-08-08 | 2013-11-20 | 董亚贤 | Cordycepsmilitaris strain liquid culture medium and preparation method therefor |
-
2015
- 2015-06-25 CN CN201510356495.6A patent/CN105039467A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935979A (en) * | 2006-11-01 | 2007-03-28 | 周永祥 | Coragyceps militaris bacterium culture method |
| CN103396193A (en) * | 2013-08-08 | 2013-11-20 | 董亚贤 | Cordycepsmilitaris strain liquid culture medium and preparation method therefor |
Non-Patent Citations (3)
| Title |
|---|
| 刘桂君等: "蛹虫草中虫草素的研究进展", 《食品科学》 * |
| 王贺祥等: "《食用菌栽培学》", 31 March 2014 * |
| 荆留萍等: "８种物质对蛹虫草液体发酵中虫草素及多糖含量的影响", 《西北农林科技大学学报（自然科学版）》 * |
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| CN105441336B (en) * | 2015-12-23 | 2019-09-10 | 上海市农业科学院 | A method of it effectively prevent filamentous fungi to degenerate and restore to produce spore |
| CN105441336A (en) * | 2015-12-23 | 2016-03-30 | 上海市农业科学院 | Method for effectively preventing degradation of filamentous fungi and restoring spore production |
| CN106381253A (en) * | 2016-08-31 | 2017-02-08 | 安康市蚕桑产业发展中心 | Process for nutritional enhancing production of cordyceps militaris yellow rice wine by cordyceps militaris rice culture material |
| CN106399124A (en) * | 2016-08-31 | 2017-02-15 | 徐春生 | Solid medium of cordyceps sinensis mycelia |
| CN106754404A (en) * | 2016-11-21 | 2017-05-31 | 王军 | A kind of Chinese caterpillar fungus culture medium for improving cordycepin content |
| CN106819007A (en) * | 2016-12-23 | 2017-06-13 | 山东建筑大学 | A kind of probiotics Pizza and preparation method thereof |
| CN106605684A (en) * | 2016-12-23 | 2017-05-03 | 山东建筑大学 | Red yeast rice pizza and preparation method thereof |
| CN106577925A (en) * | 2016-12-23 | 2017-04-26 | 山东建筑大学 | Cordyceps militaris pizza and preparation method of the same |
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN112586513B (en) * | 2020-12-11 | 2021-11-19 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN113005163A (en) * | 2021-03-25 | 2021-06-22 | 泸州品创科技有限公司 | Method for producing cordycepin by continuously culturing cordyceps militaris |
| CN113005163B (en) * | 2021-03-25 | 2023-03-14 | 泸州品创科技有限公司 | Method for producing cordycepin by continuously culturing cordyceps militaris |
| CN113151007A (en) * | 2021-03-30 | 2021-07-23 | 浙江工业大学 | Liquid fermentation medium for increasing cordycepin content in cordyceps militaris |
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