CN106754404A - A kind of Chinese caterpillar fungus culture medium for improving cordycepin content - Google Patents
A kind of Chinese caterpillar fungus culture medium for improving cordycepin content Download PDFInfo
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- CN106754404A CN106754404A CN201611070157.7A CN201611070157A CN106754404A CN 106754404 A CN106754404 A CN 106754404A CN 201611070157 A CN201611070157 A CN 201611070157A CN 106754404 A CN106754404 A CN 106754404A
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- culture medium
- juice
- caterpillar fungus
- chinese caterpillar
- potato
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Abstract
The present invention provides a kind of Chinese caterpillar fungus culture medium for improving cordycepin content, it is characterised in that including PDA enriched mediums, entity culture medium, liquid spawn culture medium, and the PDA enriched mediums composition is：The 1000ml of potato juice 500, the 25g of sucrose 15, the 250ml of cocoon pupa juice 150, the 100g of milk powder 50, the 15g of agar 10, the 10g of acidic amino acid 6, the 15g of basic amino acid 10, the 50g of ginseng pulverate 30；Culture medium preparation method of the invention is simple, it is easy to prepare, liquid spawn culture medium is included in the present invention, 98% cordycepin is secreted into culture medium in Cordyceps militaris growth course, and cordycepin is extracted from nutrient solution, and not only extract convenient, and it is with short production cycle, liquid deep drainpipe is carried out to Cordyceps militaris using the fluid nutrient medium in the present invention, the content of Cordyceps militaris cordycepin can be significantly improved.Add mangosteen powder to give full play to its bacteria resistance function in the medium, reduce the living contaminants of culture medium, improve the survival rate of strain.
Description
Technical field
The present invention relates to be related to Chinese caterpillar fungus to cultivate field, more particularly to a kind of Chinese caterpillar fungus culture medium for improving cordycepin content.
Background technology
Cordyceps sinensis also known as Chinese caterpillar fungus, are China's usual a kind of rare tonic herbs among the people, and its nutritional ingredient is higher than ginseng,
Can be used as medicine, also edible, be superior delicacies, with nutritive value very high.The main medical value of Chinese caterpillar fungus is cordycepin,
Cordycepin is a kind of important secondary metabolite that Cordyceps militaris produces, as the important bioactive ingredients of Cordyceps militaris, due to worm
Careless element has the effects such as antibacterial, antiviral, antitumor, immunological regulation, and the market demand is increasing, and price is sufficiently expensive, but
Content of the cordycepin in Cordyceps militaris is extremely low, influences its medical value, although cordycepin can be by chemical synthesis, but synthesis technique
Complexity, product recoveries are low, and production cost is high, therefore, current the best way is how to ferment to obtain Chinese caterpillar fungus by Cordyceps militaris
Element, but Chinese caterpillar fungus cultural method is simple now, and culture medium is of low nutritive value, it is impossible to which cordycepin contains in good raising Chinese caterpillar fungus
Amount, causes Chinese caterpillar fungus low with value.
The content of the invention
According to above technical problem, the present invention provides a kind of Chinese caterpillar fungus culture medium for improving cordycepin content, and its feature exists
In including PDA enriched mediums, entity culture medium, liquid spawn culture medium, the PDA enriched mediums constitute and are：Potato
Juice 500-1000ml, sucrose 15-25g, cocoon pupa juice 150-250ml, milk powder 50-100g, agar 10-15g, acidic amino acid 6-
10g, basic amino acid 10-15g, ginseng pulverate 30-50g；The liquid spawn culture medium composition is cocoon pupa powder 150-300g, brown sugar
8-20g, peptone 5-15g, soya bean juice 200-300ml, pure water 200-500ml, adenine mass concentration 0.5-1.5g/L,
Mangosteen powder 30-80g, the entity culture medium composition is millet 10-20g, rice 10-20g, barley 10-15g, liquid spawn
Culture medium 35-45ml.
The potato juice preparation method is：The clean stripping and slicing of potato cleaning that selection does not sprout is standby, then by Ma Ling
Potato and water are put into juice extractor is squeezed the juice, and squeezes the juice it is filtered after finishing, and selection potato juice is standby, the potato
It is 1 with the mass ratio of water:1-2.
The cocoon pupa juice preparation method is：Choose dried cocoon pupa to be crushed, be put into powder after crushing carries out boiling and boil in water purification
30-50min, juice is filtered to take after boiling.
The soya bean juice preparation method is：The soybean with water that will have been chosen boils 30-60min, is then filtered
Obtain soya bean juice.
The millet, rice, barley are ripe millet, cooked rice, ripe barley.
The application method of the Chinese caterpillar fungus culture medium is：
1）It is prepared by parent species：Cordyceps militaris spawn is aseptically inoculated in PDA enriched mediums, puts 23-28 DEG C of constant temperature and keep away
Optical culture, after optical culture is seen after mycelium germination, obtains original parent species；
2) liquid spawn makes：Take original parent species to be inoculated in liquid spawn culture medium, 23-28 DEG C of shaking table culture 3-5d obtains liquid
Body strain；
3) fructification culture：Fructification culture medium is sealed with polypropylene film, in 121 DEG C of -126 DEG C of steam sterilizings, is maintained
, be inoculated in liquid spawn in the fructification culture medium after sterilizing by 2-5% inoculum concentrations cultivated by 40min-60min；
4) cultivation management：Vegetative stage requirement lucifuge culture；After mycelia has thorough grasp compost completely, 15-25 DEG C of temperature,
Space humidity be 70%-75% under, carry out dim light culture；After fruit-body formation, through 8-12 DEG C of thermal stimulation, strengthen ventilation, often
Its ventilation 2 times -3 times, each 20-30min, 16-20 DEG C of temperature under space humidity 85%-95%, cultivates 40d-50d.
Beneficial effects of the present invention are：Culture medium preparation method of the invention is simple, is easy to prepare, and is carried out with basal culture medium
Chinese caterpillar fungus is cultivated, and can be very good to improve the content of cordycepin in Chinese caterpillar fungus, and Chinese caterpillar fungus is improve under conditions of low cost uses valency
Value.Liquid spawn culture medium is included in the present invention, 98% cordycepin is secreted into culture medium in Cordyceps militaris growth course, and
Cordycepin is extracted from nutrient solution, not only extracts convenient and with short production cycle, using the fluid nutrient medium in the present invention to pupa
Chinese caterpillar fungus carries out liquid deep drainpipe, can significantly improve the content of Cordyceps militaris cordycepin.Put in liquid spawn culture medium of the invention
Mangosteen powder is equipped with, growth of the Flavonoid substances in mangosteen powder to gram-positive bacteria has more significant inhibitory action,
Such as Candida albicans, aerobacteria, bacillus subtilis, Sarcina lutea.Mangosteen powder to trichoderma, Neurospora, Mucor and
Head mold also has certain inhibitory action.Add mangosteen powder to give full play to its bacteria resistance function in the medium, reduce culture medium
Living contaminants, improves the survival rate of strain.In solid medium of the invention based on millet, rice, barley, German millet nutrition is rich
Richness, particle is small, and viscosity is high after autoclaving, hinders mycelial growth.Rice, barley particles are big, and permeability is strong, and mixing with millet makes
With, millet viscosity shortcoming high is overcome, and content of fatty acid is higher in barley, and external lot of documents reports vegetable oil energy
Promote the growth of mycelia, improve the content of cordycepin.The present invention is contained within ginseng pulverate, increased medical value of the invention.
Specific embodiment
According to embodiment, the present invention is further described：
Embodiment 1
PDA enriched mediums, entity culture medium, the preparation of liquid spawn culture medium, PDA enriched mediums preparation side are carried out respectively
Method is：The clean stripping and slicing of potato cleaning for choosing non-long teeth is standby, then potato and water is put into juice extractor and is squeezed the juice,
Squeeze the juice it is filtered after finishing, selection potato juice is standby, the mass ratio of the potato and water is 1:1.5, then will
Potato juice ml, sucrose 16g, cocoon pupa juice 150ml, milk powder 60g, agar 12g, acidic amino acid 8g, basic amino acid 11g, people
Ginseng powder 35g carries out being mixed and made into the preparation of PDA enriched mediums；By cocoon pupa powder 200g, brown sugar 12g, peptone 13g, soya bean juice
230ml, pure water 400ml, adenine mass concentration 0.9g/L, mangosteen powder 40g are mixed, and are made strain cultivation
Base；Millet 15g, rice 12g, barley 13g, liquid spawn culture medium 40ml are made entity culture medium.
Culture medium is standby after the completion of preparing, and then carries out parent species preparation, and Cordyceps militaris spawn is aseptically inoculated in
In PDA enriched mediums, 23-28 DEG C of constant temperature lucifuge culture is put, after optical culture is seen after mycelium germination, obtain original parent species；Then
The original parent species for preparing are inoculated in liquid spawn culture medium, 23-28 DEG C of shaking table culture 3-5d obtains liquid spawn；Will be sub real
Body culture medium is sealed with polypropylene film, in 121 DEG C of -126 DEG C of steam sterilizings, 40min-60min is maintained, by 2-5% inoculum concentrations
Liquid spawn is inoculated in the fructification culture medium after sterilizing and is cultivated；Finally carry out cultivation management, vegetative stage requirement
Lucifuge culture；After mycelia has thorough grasp compost completely, 15-25 DEG C of temperature, space humidity be 70%-75% under, carry out dim light training
Support；After fruit-body formation, through 8-12 DEG C of thermal stimulation, strengthen ventilation, daily ventilation 2 times -3 times, each 20-30min, temperature
16-20 DEG C, under space humidity 85%-95%, cultivate 40d-50d.
The above is only the preferred embodiment of the present invention, it is noted that for the common skill of the art
For art personnel, under the premise without departing from the principles of the invention, some improvement can also be made, these improvement also should be regarded as this hair
Bright protection domain.
Claims (5)
1. it is a kind of improve cordycepin content Chinese caterpillar fungus culture medium, it is characterised in that including PDA enriched mediums, entity culture
Base, liquid spawn culture medium, the PDA enriched mediums constitute and are：Potato juice 500-1000ml, sucrose 15-25g, cocoon pupa
Juice 150-250ml, milk powder 50-100g, agar 10-15g, acidic amino acid 6-10g, basic amino acid 10-15g, ginseng pulverate 30-
50g；The liquid spawn culture medium composition is cocoon pupa powder 150-300g, brown sugar 8-20g, peptone 5-15g, soya bean juice 200-
300ml, pure water 200-500ml, adenine mass concentration 0.5-1.5g/L, mangosteen powder 30-80g, the entity culture medium
Constitute is millet 10-20g, rice 10-20g, barley 10-15g, liquid spawn culture medium 35-45ml.
2. according to a kind of Chinese caterpillar fungus culture medium for improving cordycepin content described in claim 1, it is characterised in that the potato
Juice preparation method is：The clean stripping and slicing of potato cleaning that selection does not sprout is standby, and then potato and water are put into juice extractor
Squeezed the juice, squeeze the juice it is filtered after finishing, selection potato juice is standby, the mass ratio of the potato and water is 1:1-
2。
3. according to a kind of Chinese caterpillar fungus culture medium for improving cordycepin content described in claim 1, it is characterised in that the cocoon pupa juice
Preparation method is：Choose dried cocoon pupa to be crushed, be put into powder after crushing carries out boiling and boil 30-50min in water purification, is filtered after boiling
Take juice.
4. according to a kind of Chinese caterpillar fungus culture medium for improving cordycepin content described in claim 1, it is characterised in that the soya bean juice
Preparation method is：The soybean with water that will have been chosen boils 30-60min, is then carried out filtering to take to obtain soya bean juice.
5. according to a kind of Chinese caterpillar fungus culture medium for improving cordycepin content described in claim 1, it is characterised in that the millet, big
Rice, barley are ripe millet, cooked rice, ripe barley.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611018922 | 2016-11-21 | ||
| CN2016110189220 | 2016-11-21 |
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| Publication Number | Publication Date |
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| CN106754404A true CN106754404A (en) | 2017-05-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611070157.7A Pending CN106754404A (en) | 2016-11-21 | 2016-11-29 | A kind of Chinese caterpillar fungus culture medium for improving cordycepin content |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108338040A (en) * | 2018-05-17 | 2018-07-31 | 江门市山海堂虫草有限公司 | A kind of Chinese caterpillar fungus culture medium and preparation method thereof |
| CN108812050A (en) * | 2018-05-11 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving Cordyceps militaris yield |
| CN109010382A (en) * | 2018-09-08 | 2018-12-18 | 河南欧普生物科技有限公司 | A kind of preparation method of fermentation cordyceps oral solution for animals |
| CN109136112A (en) * | 2018-10-16 | 2019-01-04 | 徐州工程学院 | A kind of method of cordycepin content in raising cordyceps mycelium |
| CN109937795A (en) * | 2019-04-01 | 2019-06-28 | 广西壮仁堂生物科技有限公司 | A method of cordyceps mycelia is cultivated by matrix of Jasmine |
| CN113068824A (en) * | 2021-04-25 | 2021-07-06 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | High-protein composite nutritional coarse cereal edible fungus powder food and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108812050A (en) * | 2018-05-11 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving Cordyceps militaris yield |
| CN108338040A (en) * | 2018-05-17 | 2018-07-31 | 江门市山海堂虫草有限公司 | A kind of Chinese caterpillar fungus culture medium and preparation method thereof |
| CN109010382A (en) * | 2018-09-08 | 2018-12-18 | 河南欧普生物科技有限公司 | A kind of preparation method of fermentation cordyceps oral solution for animals |
| CN109136112A (en) * | 2018-10-16 | 2019-01-04 | 徐州工程学院 | A kind of method of cordycepin content in raising cordyceps mycelium |
| CN109136112B (en) * | 2018-10-16 | 2019-09-13 | 徐州工程学院 | A kind of method of cordycepin content in raising cordyceps mycelium |
| CN109937795A (en) * | 2019-04-01 | 2019-06-28 | 广西壮仁堂生物科技有限公司 | A method of cordyceps mycelia is cultivated by matrix of Jasmine |
| CN113068824A (en) * | 2021-04-25 | 2021-07-06 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | High-protein composite nutritional coarse cereal edible fungus powder food and preparation method thereof |
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