CN108812050A - A kind of culture implantation methods improving Cordyceps militaris yield - Google Patents
A kind of culture implantation methods improving Cordyceps militaris yield Download PDFInfo
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- CN108812050A CN108812050A CN201810447157.7A CN201810447157A CN108812050A CN 108812050 A CN108812050 A CN 108812050A CN 201810447157 A CN201810447157 A CN 201810447157A CN 108812050 A CN108812050 A CN 108812050A
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- Prior art keywords
- cordyceps militaris
- culture
- grams
- sterilizing
- yield
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of culture implantation methods for improving Cordyceps militaris yield, include the following steps：（1）Strain prepares；（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：10 ~ 30 grams of glucose, 1 ~ 5 gram of potassium dihydrogen phosphate, 1 ~ 5 gram of magnesium sulfate, 5 ~ 15 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；Prepared fluid nutrient medium is dispensed, is sealed, sterilizing；（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；Shake culture 4 ~ 10 days；Then 20-40 grams of ginseng extract after sterilizing is added into culture solution again, 10-15 grams of corn flour, is mixed；（4）Cultural hypha；（9）Harvesting.The method of the present invention simple process and low cost can significantly improve the yield of Cordyceps militaris, significant effect in conjunction with other processing methods using specific culture solution additive in method.
Description
Technical field
The present invention relates to a kind of culture implantation methods for improving Cordyceps militaris yield, belong to Cordyceps militaris culture planting technology neck
Domain.
Background technique
Cordyceps militaris is Ascomycotina, ergot Zoopagales, Clavicipitaceae, the type sepecies of Cordyceps also known as northern Chinese caterpillar Fungus, north
Cordyceps sinensis etc..Cordyceps militaris morphological feature stroma list it is raw or it is several go out together from the head of parasitic pupal cell or section minister, color is orange
Or Chinese red, 2~8 centimetres of overall length, pupal cell color is purple, is about 1.5~2 centimetres.
Cordyceps sinensis and ginseng, pilose antler are eponymous to be three big tonics of Chinese tradition and has won fame both at home and abroad, both at home and abroad it is many specially
To Cordyceps militaris nutrient chemistry composition, the research of pharmacology and clinical effectiveness is all substantially identical with cordyceps sinensis, mainly there is guarantor's lung for family
Kidney-nourishing, hemostasis and phlegm adjust physical function, cure mainly pulmonary tuberculosis hemoptysis, impotence and seminal emission, soreness of waist and knee joint, beauty and skin care, and refreshing essence declines
Weak, diabetes rise white blood cell, and anti-cancer, anticancer are also very big to Chemotherapy.The Cordyceps militaris medicinal efficacy of artificial cultivation with it is wild
The effect of cordyceps sinensis, is very nearly the same, and the medical performance of some aspects is even better than wild Chinese caterpillar fungus.Medicine, pharmacology and clinical trial prove
Cordyceps militaris can be used as the substitute of cordyceps sinensis completely.It is had shown that through research, the Cordyceps militaris medicine clinical effectiveness manually cultivated, with
Natural cordyceps sinensis is identical, can equally adjust whole body function, improve immunocompetence, enhance macrophage phagocytic function,
Promote antibody formation, mainly have protect the nourishing the liver of lung kidney-nourishing, relieving cough and asthma eliminating the phlegm, the wrinkle resistant anti-aging of moisturizing, antimicrobial antiphlogistic, calmness,
Expansion blood vessel, reduce blood pressure, reduce blood glucose, is antifatigue, resist oxygen lack the effects of.Artificial Chinese caterpillar fungus cures mainly：Consumptive disease hemoptysis, waist and knee acid
Bitterly, impotence and seminal emission, neurasthenia, palpitation and insomnia, night sweat, arrhythmia cordis, loss of appetite, nasopharyngeal carcinoma, pulmonary emphysema, pulmonary tuberculosis, gas
The diseases such as oligoleukocythemia after Guan Yan, asthma, hepatitis, ephritis, kidney deficiency, diabetes, frequent micturition and Radiotherapy chemotherapy.
Currently, having the research of a large amount of Cordyceps militaris culture implantation methods in the prior art, but some defects are still had,
Such as Cordyceps militaris yield is not ideal enough.
Summary of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, the present invention provides a kind of Cordyceps militaris yield that improves
Cultivate implantation methods.
Technical solution：To achieve the above object, the present invention provides it is a kind of improve Cordyceps militaris yield culture implantation methods,
Include the following steps：
（1）Strain prepares：Select cordyceps militaris link bacterial strain to be seeded；
（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：10 ~ 30 grams of glucose, potassium dihydrogen phosphate
1 ~ 5 gram, 1 ~ 5 gram of magnesium sulfate, 5 ~ 15 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；By prepared liquid
Culture medium packing, is sealed, sterilizing；
（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；Concussion training
It supports 4 ~ 10 days；Then 20-40 grams of ginseng extract after sterilizing is added into culture solution again, 10-15 grams of corn flour, is mixed；
（4）Cultural hypha：By step 3）Treated culture solution is moved under dark condition and is cultivated, and is cultivated under the conditions of 18 DEG C ~ 24 DEG C
It 10 ~ 14 days, is then cultivated under illumination condition, keeps humidity 60% ~ 70%, 20 DEG C ~ 24 DEG C of temperature, cultivate 6-10 days；
（9）Harvesting：When fructification grows to 6 ~ 8 centimetres, fructification is taken out, it is dry, it is kept in dark place.
The step（1）Middle cordyceps militaris link bacterial strain be it is adaptable, it is light-exposed after annesl weeding it is fast, the stable cordyceps of character
Strain.
The step（2）The method of middle sterilizing is：It sterilizes 25 ~ 35 minutes under the conditions of 120 DEG C ~ 130 DEG C.
The step（3）The condition of middle shaken cultivation is：15 DEG C ~ 25 DEG C, training is shaken in 100 ~ 150 revs/min of shaking table
It supports.
The step（3）The preparation method of middle ginseng extract is：The boiling pure water of 6-8 times of volume of ginseng is extracted,
Each 2-4h is extracted 3 times, combined extract, is concentrated to get the water extract.
The step（4）600 ~ 800 lux of intensity of middle illumination.
Beneficial effect：Compared with the existing technology, the method for the present invention simple process and low cost, using specific training in method
Nutrient solution additive can significantly improve the yield of Cordyceps militaris, significant effect in conjunction with other processing methods.
Specific embodiment
Below with reference to embodiment, the present invention will be further explained.
Embodiment 1
（1）Strain prepares：Selection adaptation is strong, and light-exposed rear annesl weeding is fast, the stable Cordyceps militaris of character, as pupa to be seeded
Chinese caterpillar fungus strain；
（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：10 grams of glucose, potassium dihydrogen phosphate 1
Gram, 1 gram of magnesium sulfate, 5 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；By prepared fluid nutrient medium point
Dress, sealing, sterilizes 25 minutes under the conditions of 120 DEG C ~ 130 DEG C；
（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；15 DEG C,
Shake culture 4 days in 150 revs/min of shaking table；Then 20 grams of ginseng extract after sterilizing are added into culture solution again, jade
It 10 grams of rice flour, mixes；The preparation method of the ginseng extract is：The boiling pure water of 6 times of volumes of ginseng is extracted, every time
4h is extracted 3 times, combined extract, is concentrated to get the water extract；
（4）Cultural hypha：By step 3）Treated culture solution is moved under dark condition and is cultivated, and is cultivated 14 days under the conditions of 18 DEG C,
Then it is cultivated under the conditions of 600 lux illuminations, keeps humidity 60% ~ 70%, 20 DEG C ~ 24 DEG C of temperature, cultivate 6 days；
（9）Harvesting：When fructification grows to 6 ~ 8 centimetres, fructification is taken out, it is dry, it is kept in dark place.
Embodiment 2
（1）Strain prepares：Selection adaptation is strong, and light-exposed rear annesl weeding is fast, the stable Cordyceps militaris of character, as pupa to be seeded
Chinese caterpillar fungus strain；
（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：30 grams of glucose, potassium dihydrogen phosphate 5
Gram, 5 grams of magnesium sulfate, 15 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；By prepared fluid nutrient medium point
Dress, sealing, sterilizes 35 minutes under the conditions of 120 DEG C ~ 130 DEG C；
（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；25 DEG C,
Shake culture 10 days in 100 revs/min of shaking table；Then 40 grams of ginseng extract after sterilizing are added into culture solution again, jade
It 15 grams of rice flour, mixes；The preparation method of the ginseng extract is：The boiling pure water of 8 times of volumes of ginseng is extracted, each 2h,
It extracts 3 times, combined extract, is concentrated to get the water extract；
（4）Cultural hypha：By step 3）Treated culture solution is moved under dark condition and is cultivated, and is cultivated 10 days under the conditions of 24 DEG C,
Then it is cultivated under the conditions of 800 lux illuminations, keeps humidity 60% ~ 70%, 20 DEG C ~ 24 DEG C of temperature, cultivate 10 days；
（9）Harvesting：When fructification grows to 6 ~ 8 centimetres, fructification is taken out, it is dry, it is kept in dark place.
Embodiment 3
（1）Strain prepares：Selection adaptation is strong, and light-exposed rear annesl weeding is fast, the stable Cordyceps militaris of character, as pupa to be seeded
Chinese caterpillar fungus strain；
（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：20 grams of glucose, potassium dihydrogen phosphate 3
Gram, 3 grams of magnesium sulfate, 10 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；By prepared fluid nutrient medium point
Dress, sealing, sterilizes 30 minutes under the conditions of 120 DEG C ~ 130 DEG C；
（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；20 DEG C,
Shake culture 7 days in 120 revs/min of shaking table；Then 30 grams of ginseng extract after sterilizing are added into culture solution again, jade
It 12 grams of rice flour, mixes；The preparation method of the ginseng extract is：The boiling pure water of 7 times of volumes of ginseng is extracted, every time
3h is extracted 3 times, combined extract, is concentrated to get the water extract；
（4）Cultural hypha：By step 3）Treated culture solution is moved under dark condition and is cultivated, and is cultivated 12 days under the conditions of 21 DEG C,
Then it is cultivated under the conditions of 700 lux illuminations, keeps humidity 60% ~ 70%, 20 DEG C ~ 24 DEG C of temperature, cultivate 8 days；
（9）Harvesting：When fructification grows to 6 ~ 8 centimetres, fructification is taken out, it is dry, it is kept in dark place.
Embodiment 4
（1）Strain prepares：Selection adaptation is strong, and light-exposed rear annesl weeding is fast, the stable Cordyceps militaris of character, as pupa to be seeded
Chinese caterpillar fungus strain；
（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：15 grams of glucose, potassium dihydrogen phosphate 2
Gram, 2 grams of magnesium sulfate, 8 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；By prepared fluid nutrient medium point
Dress, sealing, sterilizes 28 minutes under the conditions of 120 DEG C ~ 130 DEG C；
（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；18 DEG C,
Shake culture 5 days in 120 revs/min of shaking table；Then 25 grams of ginseng extract after sterilizing are added into culture solution again, jade
It 12 grams of rice flour, mixes；The preparation method of the ginseng extract is：The boiling pure water of 7 times of volumes of ginseng is extracted, every time
3h is extracted 3 times, combined extract, is concentrated to get the water extract；
（4）Cultural hypha：By step 3）Treated culture solution is moved under dark condition and is cultivated, and is cultivated 11 days under the conditions of 20 DEG C,
Then it is cultivated under the conditions of 650 lux illuminations, keeps humidity 60% ~ 70%, 20 DEG C ~ 24 DEG C of temperature, cultivate 7 days；
（9）Harvesting：When fructification grows to 6 ~ 8 centimetres, fructification is taken out, it is dry, it is kept in dark place.
Embodiment 5
（1）Strain prepares：Selection adaptation is strong, and light-exposed rear annesl weeding is fast, the stable Cordyceps militaris of character, as pupa to be seeded
Chinese caterpillar fungus strain；
（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：25 grams of glucose, 4 grams of potassium dihydrogen phosphate,
4 grams of magnesium sulfate, 12 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；Prepared fluid nutrient medium is dispensed, is sealed
Mouthful, it sterilizes 32 minutes under the conditions of 120 DEG C ~ 130 DEG C；
（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；22 DEG C,
Shake culture 8 days in 140 revs/min of shaking table；Then 35 grams of ginseng extract after sterilizing are added into culture solution again, jade
It 14 grams of rice flour, mixes；The preparation method of the ginseng extract is：The boiling pure water of 7 times of volumes of ginseng is extracted, every time
3h is extracted 3 times, combined extract, is concentrated to get the water extract；
（4）Cultural hypha：By step 3）Treated culture solution is moved under dark condition and is cultivated, and is cultivated 13 days under the conditions of 22 DEG C,
Then it is cultivated under the conditions of 750 lux illuminations, keeps humidity 60% ~ 70%, 20 DEG C ~ 24 DEG C of temperature, cultivate 9 days；
（9）Harvesting：When fructification grows to 6 ~ 8 centimetres, fructification is taken out, it is dry, it is kept in dark place.
Comparative example 1
It is identical as the embodiment of the present invention 3, the difference is that step（3）Ginseng extract is added without in culture solution, other are homogeneous
Together, final Cordyceps militaris yield compared with Example 3, declines 15.5%.
Comparative example 2
It is identical as the embodiment of the present invention 3, the difference is that step（3）Ginseng extract is added without in culture solution, other are homogeneous
Together, final Cordyceps militaris yield compared with Example 3, declines 16.3%.
Comparative example 3
It is identical as the embodiment of the present invention 3, the difference is that step（3）Ginseng extract is added without in culture solution, other are homogeneous
Together, final Cordyceps militaris yield compared with Example 3, declines 15.8%.
Claims (6)
1. a kind of culture implantation methods for improving Cordyceps militaris yield, which is characterized in that include the following steps：
（1）Strain prepares：Select cordyceps militaris link bacterial strain to be seeded；
（2）Culture medium prepares：Fluid nutrient medium is prepared, recipe ingredient is by weight：10 ~ 30 grams of glucose, potassium dihydrogen phosphate
1 ~ 5 gram, 1 ~ 5 gram of magnesium sulfate, 5 ~ 15 grams of peptone, 150 milligrams of vitamin B, 1000 milliliters of water；By prepared liquid
Culture medium packing, is sealed, sterilizing；
（3）Inoculation：Culture medium is taken out after sterilizing, cooled to room temperature aseptically accesses Cordyceps militaris spawn；Concussion training
It supports 4 ~ 10 days；Then 20-40 grams of ginseng extract after sterilizing is added into culture solution again, 10-15 grams of corn flour, is mixed；
（4）Cultural hypha：By step 3）Treated culture solution is moved under dark condition and is cultivated, and is cultivated under the conditions of 18 DEG C ~ 24 DEG C
It 10 ~ 14 days, is then cultivated under illumination condition, keeps humidity 60% ~ 70%, 20 DEG C ~ 24 DEG C of temperature, cultivate 6-10 days；
（9）Harvesting：When fructification grows to 6 ~ 8 centimetres, fructification is taken out, it is dry, it is kept in dark place.
2. the culture implantation methods according to claim 1 for improving Cordyceps militaris yield, which is characterized in that the step（1）
Middle cordyceps militaris link bacterial strain be it is adaptable, it is light-exposed after annesl weeding it is fast, the stable cordyceps militaris link bacterial strain of character.
3. the culture implantation methods according to claim 1 for improving Cordyceps militaris yield, which is characterized in that the step（2）
The method of middle sterilizing is：It sterilizes 25 ~ 35 minutes under the conditions of 120 DEG C ~ 130 DEG C.
4. the culture implantation methods according to claim 1 for improving Cordyceps militaris yield, which is characterized in that the step（3）
The condition of middle shaken cultivation is：15 DEG C ~ 25 DEG C, shake culture in 100 ~ 150 revs/min of shaking table.
5. the culture implantation methods according to claim 1 for improving Cordyceps militaris yield, which is characterized in that the step（3）
The preparation method of middle ginseng extract is：The boiling pure water of 6-8 times of volume of ginseng is extracted, each 2-4h is extracted 3 times, is closed
And extracting solution, it is concentrated to get the water extract.
6. the culture implantation methods according to claim 1 for improving Cordyceps militaris yield, which is characterized in that the step（4）
600 ~ 800 lux of intensity of middle illumination.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858673A (en) * | 2014-03-18 | 2014-06-18 | 鲁东大学 | Method for planting cordyceps militaris by beer residue |
| CN103430776B (en) * | 2013-09-04 | 2014-12-10 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
| CN104285820A (en) * | 2014-10-13 | 2015-01-21 | 海南森瑞谱生命科学药业股份有限公司 | Selenium-rich cordyceps militaris strain and application thereof |
| CN104756759A (en) * | 2015-03-17 | 2015-07-08 | 沁阳市西向食用菌研究所 | Dioscorea opposita cordyceps sinensis and culture method thereof |
| CN104871830A (en) * | 2015-06-15 | 2015-09-02 | 鲁东大学 | Method for planting cordyceps militaris with bean worms as carriers |
| CN105875198A (en) * | 2016-04-29 | 2016-08-24 | 安发（福建）生物科技有限公司 | Culture method for improving stability of cordyceps militaris strain |
| CN106754404A (en) * | 2016-11-21 | 2017-05-31 | 王军 | A kind of Chinese caterpillar fungus culture medium for improving cordycepin content |
| CN107333568A (en) * | 2017-09-19 | 2017-11-10 | 张家港市藏联生物研究所有限公司 | A kind of more batches of culture techniques of Cordyceps militaris constant temperature |
-
2018
- 2018-05-11 CN CN201810447157.7A patent/CN108812050A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430776B (en) * | 2013-09-04 | 2014-12-10 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
| CN103858673A (en) * | 2014-03-18 | 2014-06-18 | 鲁东大学 | Method for planting cordyceps militaris by beer residue |
| CN104285820A (en) * | 2014-10-13 | 2015-01-21 | 海南森瑞谱生命科学药业股份有限公司 | Selenium-rich cordyceps militaris strain and application thereof |
| CN104756759A (en) * | 2015-03-17 | 2015-07-08 | 沁阳市西向食用菌研究所 | Dioscorea opposita cordyceps sinensis and culture method thereof |
| CN104871830A (en) * | 2015-06-15 | 2015-09-02 | 鲁东大学 | Method for planting cordyceps militaris with bean worms as carriers |
| CN105875198A (en) * | 2016-04-29 | 2016-08-24 | 安发（福建）生物科技有限公司 | Culture method for improving stability of cordyceps militaris strain |
| CN106754404A (en) * | 2016-11-21 | 2017-05-31 | 王军 | A kind of Chinese caterpillar fungus culture medium for improving cordycepin content |
| CN107333568A (en) * | 2017-09-19 | 2017-11-10 | 张家港市藏联生物研究所有限公司 | A kind of more batches of culture techniques of Cordyceps militaris constant temperature |
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Application publication date: 20181116 |