CN103858673A - Method for planting cordyceps militaris by beer residue - Google Patents
Method for planting cordyceps militaris by beer residue Download PDFInfo
- Publication number
- CN103858673A CN103858673A CN201410098406.8A CN201410098406A CN103858673A CN 103858673 A CN103858673 A CN 103858673A CN 201410098406 A CN201410098406 A CN 201410098406A CN 103858673 A CN103858673 A CN 103858673A
- Authority
- CN
- China
- Prior art keywords
- bottle
- beer
- milliliters
- grams
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a method for planting cordyceps militaris by beer residue. The method comprises the steps of beer residue drying, liquid strain preparation, nutrition liquid preparation, sterilization, inoculation, dark culture, color changing, fruit body growing, harvesting and the like. The method has the advantages that the beer residue is successfully utilized for planting the cordyceps militaris, and the biotransformation rate of the beer residue is greatly improved, so nutrition substances in the beer residue are utilized to the maximum degree, and the effects of no pollution and no waste in the production process are really realized.
Description
Technical field
The present invention relates to a kind of method of planting Cordyceps militaris.
Background technology
Beer slag is the main by product of brewing industry, is take barley as raw material, extracts the residue after soluble-carbohydrate in seed through fermentation.The main component of beer slag is malt husks and residual starch, protein, amino acid and trace element etc., after the series of process such as dehydration, fermentation are processed, can from beer slag, obtain containing protein and reach 50% to 70% malt albumen, be used at present cultivation aspect, can directly be used for keeping fowls and pig, ox, sheep, etc. domestic animal.Along with economic development and people's living standard improve, to beer, consumption also increases year by year, and as you know, China has been first Beer Brewage state in the world at present, therefore, in producing beer, has also produced a large amount of beer slags.How better this nutritious industrial residue of development and utilization, become everybody and fallen over each other the problem of studying.
Summary of the invention
The object of the present invention is to provide a kind of biological transformation ratio that improves beer slag, the method with beer slag plantation Cordyceps militaris that makes the nutriment in beer slag obtain utilizing to greatest extent.
Technical solution of the present invention is:
By a method for beer slag plantation Cordyceps militaris, it is characterized in that: comprise the following steps:
(1) oven dry of beer slag: beer slag is placed in the baking oven of 60 ℃ to drying and processing 1.5 ~ 2.5 hours;
(2) preparation of liquid spawn: the liquid nutrient medium preparing: the recipe ingredient of liquid nutrient medium is by weight: 10 ~ 30 grams of glucose, 1 ~ 5 gram of potassium dihydrogen phosphate, 1 ~ 5 gram, magnesium sulfate, 5 ~ 15 grams of peptones, 150 milligrams of Cobastabs, 1000 milliliters, water; The liquid nutrient medium preparing is sub-packed in conical flask, 250 milliliters every bottle, sealing, sterilizing 25 ~ 35 minutes under 120 ℃ ~ 130 ℃ conditions; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 15 ℃ ~ 25 ℃, in the shaking table of 110 ~ 140 revs/min, concussion is cultivated 4 ~ 10 days;
(3) preparation of nutrient solution: the recipe ingredient of nutrient solution is by weight: 25 ~ 40 grams of glucose, 1 ~ 5 gram of potassium dihydrogen phosphate, 1 ~ 5 gram, magnesium sulfate, 15 ~ 25 grams of peptones, 150 milligrams of Cobastabs, 1000 milliliters, water;
(4) sterilizing: with the wide-mouth bottle of 500 milliliters, every bottle packs composts or fertilisers of cultivating in following ratio: 30 grams of the dry beer schlempes that step (1) obtains, 10 grams of corn flour, 40 ~ 50 milliliters of nutrient solutions prepared by step (3), with covering tightly with the lid of ventilation hole or tightening bottleneck with polyethylene plastic film, bottle is placed under 120 ℃ ~ 130 ℃ conditions to sterilizing 25 ~ 35 minutes, takes out to be placed on and on superclean bench, be cooled to room temperature;
(5) inoculation: every bottle graft enters liquid spawn under aseptic condition, sealing;
(6) dark culturing: postvaccinal blake bottle is moved under dark condition and cultivated, start temperature keep 18 ℃ ~ 20 ℃ 4 ~ 6 days, after blake bottle surface covers with white hypha, temperature is risen under 20 ℃ ~ 24 ℃ conditions and cultivate again 8 ~ 12 days, after mycelia is had thorough grasp medium, blake bottle is moved on under light and cultivated;
(7) annesl: keep 20 ℃ ~ 24 ℃ of indoor temperatures, humidity 60% ~ 70%, intensity of illumination 800 luxs, 12 ~ 14 hours every days of light application time, cultivate mycelia after 5 ~ 8 days and transfer gradually crocus to by white, afterwards nocturnal temperature is down to 10 ℃ ~ 15 ℃, keep there is round the clock the temperature difference of 5 ~ 10 ℃, in the time that mycelia surface is covered with the projection of grain of rice size, stops day and night temperature and transfer 20 ℃ ~ 24 ℃ constant temperature culture to;
(8) growth of fruit body: keep 20 ℃ ~ 24 ℃ of indoor temperatures, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 luxs, 12 ~ 14 hours every days of light application time, after fruit body grows to 3 ~ 4 centimetres, indoor humidity is brought up to 75% ~ 85%, temperature and light application time are constant;
(9) gather: in the time that fruit body grows to 6 ~ 8 centimetres, fruit body is taken out from blake bottle, be placed in aeration-drying place natural air drying or 60 ℃ of baking ovens and dry, be contained in airtight plastic sack and keep in Dark Place.
Beer slag in step (1) is young beer slag.
In step (5), the access amount of liquid spawn is 5 milliliters.
The present invention successfully utilizes beer slag to plant out Cordyceps militaris, greatly improve the biological transformation ratio of beer slag, make the nutriment in beer slag obtain utilizing to greatest extent, and the cordycepic acid content in culture medium of Cordyceps militaris is 3.95%, cordycepin 0.78% and Cordyceps sinensis polysaccharide 11.8%, can be pulverized as feed addictive, greatly be improved the immunocompetence of domestic birds and animals.Really accomplish that production process is pollution-free, without discarded object.
Below in conjunction with embodiment, the invention will be further described.
By a method for beer slag plantation Cordyceps militaris, it is characterized in that: comprise the following steps:
(1) oven dry of beer slag: young beer slag is placed in the baking oven of 60 ℃ to drying and processing 1.5 ~ 2.5 hours;
(2) preparation of liquid spawn: the liquid nutrient medium preparing: the recipe ingredient of liquid nutrient medium is by weight: 10 ~ 30 grams of glucose, 1 ~ 5 gram of potassium dihydrogen phosphate, 1 ~ 5 gram, magnesium sulfate, 5 ~ 15 grams of peptones, 150 milligrams of Cobastabs, 1000 milliliters, water; The liquid nutrient medium preparing is sub-packed in conical flask, 250 milliliters every bottle, sealing, sterilizing 25 ~ 35 minutes under 120 ℃ ~ 130 ℃ conditions; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 15 ℃ ~ 25 ℃, in the shaking table of 110 ~ 140 revs/min, concussion is cultivated 4 ~ 10 days;
(3) preparation of nutrient solution: the recipe ingredient of nutrient solution is by weight: 25 ~ 40 grams of glucose, 1 ~ 5 gram of potassium dihydrogen phosphate, 1 ~ 5 gram, magnesium sulfate, 15 ~ 25 grams of peptones, 150 milligrams of Cobastabs, 1000 milliliters, water;
(4) sterilizing: with the wide-mouth bottle of 350 milliliters, every bottle packs composts or fertilisers of cultivating in following ratio: 30 grams of the dry beer schlempes that step (1) obtains, 10 grams of corn flour, 40 ~ 50 milliliters of nutrient solutions prepared by step (3), with covering tightly with the lid of ventilation hole or tightening bottleneck with polyethylene plastic film, bottle is placed under 120 ℃ ~ 130 ℃ conditions to sterilizing 25 ~ 35 minutes, takes out to be placed on and on superclean bench, be cooled to room temperature;
(5) inoculation: every bottle graft enters 5 milliliters of liquid bacterial classifications under aseptic condition, sealing;
(6) dark culturing: postvaccinal blake bottle is moved under dark condition and cultivated, start temperature keep 18 ℃ ~ 20 ℃ 4 ~ 6 days, after blake bottle surface covers with white hypha, temperature is risen under 20 ℃ ~ 24 ℃ conditions and cultivate again 8 ~ 12 days, after mycelia is had thorough grasp medium, blake bottle is moved on under light and cultivated;
(7) annesl: keep 20 ℃ ~ 24 ℃ of indoor temperatures, humidity 60% ~ 70%, intensity of illumination 800 luxs, 12 ~ 14 hours every days of light application time, cultivate mycelia after 5 ~ 8 days and transfer gradually crocus to by white, afterwards nocturnal temperature is down to 10 ℃ ~ 15 ℃, keep there is round the clock the temperature difference of 5 ~ 10 ℃, in the time that mycelia surface is covered with the projection of grain of rice size, stops day and night temperature and transfer 20 ℃ ~ 24 ℃ constant temperature culture to;
(8) growth of fruit body: keep 20 ℃ ~ 24 ℃ of indoor temperatures, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 luxs, 12 ~ 14 hours every days of light application time, after fruit body grows to 3 ~ 4 centimetres, indoor humidity is brought up to 75% ~ 85%, temperature and light application time are constant;
(9) gather: in the time that fruit body grows to 6 ~ 8 centimetres, fruit body is pressed from both sides out from blake bottle with tweezers, be placed in aeration-drying place natural air drying or 60 ℃ of baking ovens and dry, be contained in airtight plastic sack and keep in Dark Place.
(10) medium is dried after pulverizing, can be used as feed additive production animal feeding-stuff.
Claims (3)
1. by a method for beer slag plantation Cordyceps militaris, it is characterized in that: comprise the following steps:
(1) oven dry of beer slag: beer slag is placed in the baking oven of 60 ℃ to drying and processing 1.5 ~ 2.5 hours;
(2) preparation of liquid spawn: the liquid nutrient medium preparing: the recipe ingredient of liquid nutrient medium is by weight: 10 ~ 30 grams of glucose, 1 ~ 5 gram of potassium dihydrogen phosphate, 1 ~ 5 gram, magnesium sulfate, 5 ~ 15 grams of peptones, 150 milligrams of Cobastabs, 1000 milliliters, water; The liquid nutrient medium preparing is sub-packed in conical flask, 250 milliliters every bottle, sealing, sterilizing 25 ~ 35 minutes under 120 ℃ ~ 130 ℃ conditions; After sterilizing, take out medium, naturally cool to room temperature, under aseptic condition, access Cordyceps militaris spawn; At 15 ℃ ~ 25 ℃, in the shaking table of 110 ~ 140 revs/min, concussion is cultivated 4 ~ 10 days;
(3) preparation of nutrient solution: the recipe ingredient of nutrient solution is by weight: 25 ~ 40 grams of glucose, 1 ~ 5 gram of potassium dihydrogen phosphate, 1 ~ 5 gram, magnesium sulfate, 15 ~ 25 grams of peptones, 150 milligrams of Cobastabs, 1000 milliliters, water;
(4) sterilizing: with the wide-mouth bottle of 500 milliliters, every bottle packs composts or fertilisers of cultivating in following ratio: 30 grams of the dry beer schlempes that step (1) obtains, 10 grams of corn flour, 40 ~ 50 milliliters of nutrient solutions prepared by step (3), with covering tightly with the lid of ventilation hole or tightening bottleneck with polyethylene plastic film, bottle is placed under 120 ℃ ~ 130 ℃ conditions to sterilizing 25 ~ 35 minutes, takes out to be placed on and on superclean bench, be cooled to room temperature;
(5) inoculation: every bottle graft enters liquid spawn under aseptic condition, sealing;
(6) dark culturing: postvaccinal blake bottle is moved under dark condition and cultivated, start temperature keep 18 ℃ ~ 20 ℃ 4 ~ 6 days, after blake bottle surface covers with white hypha, temperature is risen under 20 ℃ ~ 24 ℃ conditions and cultivate again 8 ~ 12 days, after mycelia is had thorough grasp medium, blake bottle is moved on under light and cultivated;
(7) annesl: keep 20 ℃ ~ 24 ℃ of indoor temperatures, humidity 60% ~ 70%, intensity of illumination 800 luxs, 12 ~ 14 hours every days of light application time, cultivate mycelia after 5 ~ 8 days and transfer gradually crocus to by white, afterwards nocturnal temperature is down to 10 ℃ ~ 15 ℃, keep there is round the clock the temperature difference of 5 ~ 10 ℃, in the time that mycelia surface is covered with the projection of grain of rice size, stops day and night temperature and transfer 20 ℃ ~ 24 ℃ constant temperature culture to;
(8) growth of fruit body: keep 20 ℃ ~ 24 ℃ of indoor temperatures, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 luxs, 12 ~ 14 hours every days of light application time, after fruit body grows to 3 ~ 4 centimetres, indoor humidity is brought up to 75% ~ 85%, temperature and light application time are constant;
(9) gather: in the time that fruit body grows to 6 ~ 8 centimetres, fruit body is taken out from blake bottle, be placed in aeration-drying place natural air drying or 60 ℃ of baking ovens and dry, be contained in airtight plastic sack and keep in Dark Place.
2. the method with beer slag plantation Cordyceps militaris according to claim 1, is characterized in that: the beer slag in step (1) is young beer slag.
3. the method with beer slag plantation Cordyceps militaris according to claim 1, is characterized in that: in step (5), the access amount of liquid spawn is 5 milliliters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410098406.8A CN103858673B (en) | 2014-03-18 | 2014-03-18 | Method for planting cordyceps militaris by beer residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410098406.8A CN103858673B (en) | 2014-03-18 | 2014-03-18 | Method for planting cordyceps militaris by beer residue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103858673A true CN103858673A (en) | 2014-06-18 |
| CN103858673B CN103858673B (en) | 2015-04-08 |
Family
ID=50898140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410098406.8A Active CN103858673B (en) | 2014-03-18 | 2014-03-18 | Method for planting cordyceps militaris by beer residue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103858673B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498367A (en) * | 2014-11-28 | 2015-04-08 | 沈阳源康生物技术发展有限公司 | Medium for cordyceps militaris liquid deep fermentation and use method thereof |
| CN105779300A (en) * | 2016-02-22 | 2016-07-20 | 嘉兴市潜福食品有限公司 | Solid culture medium and cultivation method of cordyceps militaris fruiting bodies |
| CN105941628A (en) * | 2016-05-03 | 2016-09-21 | 内蒙古鼎祥健康产业投资有限公司 | Preparation method of health-care Chinese caterpillar fungus peptide milk for middle-aged and aged people |
| CN106069177A (en) * | 2016-06-12 | 2016-11-09 | 鲁东大学 | The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content |
| CN107114119A (en) * | 2017-05-20 | 2017-09-01 | 天津市东方中滨农业科技有限公司 | A kind of method that use thermal stimulation cultivates Cordyceps militaris |
| CN108633616A (en) * | 2018-05-11 | 2018-10-12 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving activity substance content in Cordyceps militaris |
| CN108812050A (en) * | 2018-05-11 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving Cordyceps militaris yield |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946638A (en) * | 2010-09-20 | 2011-01-19 | 福建省曲斗香酒业有限公司 | Method for culturing cordyceps militaris by distilled grain |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
-
2014
- 2014-03-18 CN CN201410098406.8A patent/CN103858673B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946638A (en) * | 2010-09-20 | 2011-01-19 | 福建省曲斗香酒业有限公司 | Method for culturing cordyceps militaris by distilled grain |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
Non-Patent Citations (1)
| Title |
|---|
| 徐冲等: "蛹虫草菌丝固体法培养初探", 《山东农业科学》, no. 11, 31 December 2010 (2010-12-31), pages 66 - 68 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498367A (en) * | 2014-11-28 | 2015-04-08 | 沈阳源康生物技术发展有限公司 | Medium for cordyceps militaris liquid deep fermentation and use method thereof |
| CN105779300A (en) * | 2016-02-22 | 2016-07-20 | 嘉兴市潜福食品有限公司 | Solid culture medium and cultivation method of cordyceps militaris fruiting bodies |
| CN105941628A (en) * | 2016-05-03 | 2016-09-21 | 内蒙古鼎祥健康产业投资有限公司 | Preparation method of health-care Chinese caterpillar fungus peptide milk for middle-aged and aged people |
| CN106069177A (en) * | 2016-06-12 | 2016-11-09 | 鲁东大学 | The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content |
| CN107114119A (en) * | 2017-05-20 | 2017-09-01 | 天津市东方中滨农业科技有限公司 | A kind of method that use thermal stimulation cultivates Cordyceps militaris |
| CN108633616A (en) * | 2018-05-11 | 2018-10-12 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving activity substance content in Cordyceps militaris |
| CN108812050A (en) * | 2018-05-11 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving Cordyceps militaris yield |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103858673B (en) | 2015-04-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103858673B (en) | Method for planting cordyceps militaris by beer residue | |
| CN101444170B (en) | Strain separation method of apricot ormer mushroom and cultivating method thereof | |
| CN102577840B (en) | Method for cultivating edible fungus with vinegar residue | |
| CN102037858B (en) | Method for industrially culturing needle mushroom by utilizing soybean stalks | |
| CN102870600B (en) | Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content | |
| CN104557270A (en) | Black fungus culture method and culture medium suitable for northeast region | |
| CN102047811A (en) | High-quality and high-yield culturing method of agrocybe aegerita | |
| CN103283608B (en) | Factory cultivation strains of needle mushrooms, and cultivation method thereof | |
| CN104303830A (en) | Planting method of Pleurotus eryngii | |
| CN104160872B (en) | A kind of method for planting almond abalone mushroom of safety and environmental protection | |
| CN104871830A (en) | Method for planting cordyceps militaris with bean worms as carriers | |
| CN105009940A (en) | Method for culturing enoki mushroom with herb residues | |
| CN105145116A (en) | Ganoderma lucidum cultivation method for enriching trace mineral elements such as selenium and zinc | |
| CN107135805A (en) | A kind of asparagus ecology planting method based on high nutrition | |
| CN105884464A (en) | Edible fungus cultivation matrix and manufacturing method thereof | |
| CN103875457B (en) | A kind of production method improving bag cultivation pleurotus eryngii fruiting quality | |
| CN103385115B (en) | Technology for planting straw mushroom by taking sisal hemp slag as raw material | |
| CN106831082A (en) | Improve the planting material and its cultural method of clitocybe maxima yield and amino acid content | |
| CN110214628A (en) | A kind of greenhouse cultivation method of oyster mushroom | |
| CN104663247A (en) | Method for utilizing mulberry branch powder to cultivate agrocybe cylindracea | |
| CN104177182A (en) | Cultural method for producing cordyceps militaris fruiting body by use of corn cobs | |
| CN106565340A (en) | Cultivation material for improving agaricus bisporus yield and amino acid content and cultivating method of cultivation material | |
| CN105340576A (en) | White flammulina velutipes cultivation method | |
| CN102657032A (en) | Method for increasing yield of fruiting bodies of cordyceps militaris | |
| CN104762218B (en) | A kind of phalaenopsis ear edible fungus species and its implantation methods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |