CN106069177A - The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content - Google Patents
The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content Download PDFInfo
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- CN106069177A CN106069177A CN201610404341.4A CN201610404341A CN106069177A CN 106069177 A CN106069177 A CN 106069177A CN 201610404341 A CN201610404341 A CN 201610404341A CN 106069177 A CN106069177 A CN 106069177A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The invention discloses the cultural method of the Cordyceps sporophore of a kind of selenium-rich high cordycepin content, include the activation of strain, the preparation of liquid spawn successively, prepare rice medium, sterilizing, inoculation, mycelial cultivation, annesl, the cultivation of sporophore, the step such as gather.The content of the Cordyceps sporophore that the present invention turns out not only selenium element is high, moreover it is possible to make the Cordyceps medical value turned out higher and safety good.
Description
Technical field
The present invention relates to the cultural method of a kind of Cordyceps sporophore.
Background technology
Cultivation to Cordyceps sporophore in prior art, general to use sodium selenite be selenium source, has certain dangerous
Property.
White beche-de-mer has another name called white jade ginseng, is the rarest Stichopus japonicus, is by Radix Morinae Bulleyanae hereditary variation, the most rare at nature
See.It is up to 630 microgram/100 gram according to the content checking its internal selenium, is 10 times of common Stichopus japonicus, and more than 90% is organic
Selenium." king of anti-cancer " that selenium is generally referred to as in body trace element by scientist at present, therefore the growth of tumor is had by white beche-de-mer
Good inhibiting effect, can effective antitumor.It addition, the various constituent content such as calcium, zinc is the highest in Stichopus japonicus, protein, various ammonia
Base acid content is enriched, and especially can supplement various essential amino acids in human body.
Summary of the invention
The content that it is an object of the invention to provide a kind of Cordyceps sporophore turned out not only selenium element is high, moreover it is possible to make training
The cultural method of the Cordyceps sporophore of the selenium-rich high cordycepin content that Cordyceps medical value is higher and safety is good raised.
The technical solution of the present invention is:
The cultural method of the Cordyceps sporophore of a kind of selenium-rich high cordycepin content, is characterized in that: comprise the following steps: successively
(1) activation of strain: Cordyceps militaris spawn is connected in the fluid medium of following components and activates and optimize: glucose
15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram, vitamin B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram,
Yeast 3-5 gram of powder of leaching, agar powder 15-20 gram, add water to 1000 milliliters；By aforesaid liquid culture medium at 121 DEG C, 0.1MPa condition
Lower sterilizing 30 minutes, pours in culture dish after cooling；Cordyceps militaris spawn is accessed in culture dish, cultivate under 22 DEG C of dark conditions
5-10 days, bacterium colony was transferred on slant medium again, cultivated 5-10 days under 22 DEG C of dark conditions, the most required Cordyceps of gained strain
Kind；
(2) preparation of liquid spawn:
First preparing nutritional solution A: glucose 15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram by following formula, dimension is raw
Element B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram, yeast 3-5 gram of powder of leaching, add water to 1000 milliliters；Nutritional solution A is divided
Being contained in the conical flask of 500 milliliters, 250 milliliters every bottle, sealed membrane seals, at 121 DEG C, under the conditions of 0.1MPa, and sterilizing 30 minutes,
On super-clean bench, access the cordyceps species activated through step (1) after cooling, then at 130 revs/min, the shaking table of 22 DEG C shakes
Cultivate 5-9 days；
(3) rice medium is prepared:
First configure nutritional solution B: glucose 15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram, vitamin by formula as below
B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram, yeast 3-5 gram of powder of leaching, add water to 1000 milliliters；
The preparation of rice medium: with the wide-mouth culture bottle of 350 milliliters, every bottle in following ratio loading compost: rice 35-40
Gram, nutritional solution B 40 ~ 50 milliliters, cover tightly with the lid of band passage or tighten culture bottle bottleneck with polyethylene plastic film；
(4) sterilizing: culture bottle is placed on 121 DEG C, under the conditions of 0.1MPa, sterilizing 30 minutes；
(5) inoculation: aseptically, each culture bottle accesses 5 milliliters of liquid strains, sealing；
(6) mycelial cultivation: postvaccinal culture bottle is moved under dark condition and cultivate；Start temperature and keep 16 DEG C ~ 18 DEG C
4 ~ 6 days；It is further cultured under the conditions of temperature being risen to 20 DEG C ~ 24 DEG C after culture bottle surface covers with white hypha 4 ~ 6 days, treats that mycelia eats
Thoroughly after culture medium, move on to culture bottle cultivate under light；
(7) annesl: keep indoor temperature 20 DEG C ~ 22 DEG C, humidity 60% ~ 70%, intensity of illumination 800 lux, light application time every day
12 ~ 14 hours；After being cultivated 3 ~ 5 days by culture bottle, visible mycelia is gradually transferred to crocus by white, is down to by nocturnal temperature afterwards
10 DEG C ~ 15 DEG C temperature difference keeping having 5 ~ 10 DEG C round the clock, when hyphal surface is covered with the projection of grain of rice size, stop day and night temperature and turn
For constant temperature culture；
(8) cultivation of sporophore: holding indoor temperature 20 DEG C ~ 22 DEG C, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 lux,
12 ~ 14 hours every days of light application time, indoor humidity is brought up to 75% ~ 85%, temperature and illumination after sporophore grows to 3 ~ 4 centimetres
Time is constant；
(9) gather: when sporophore grows to 6 ~ 8 centimetres, with tweezers, sporophore is pressed from both sides out from culture bottle, be placed at aeration-drying
Natural air drying or 65 DEG C of baking ovens are dried, dry sporophore is contained in airtight plastic bag and keeps in Dark Place.
When in step (1), bacterium colony is transferred on slant medium, being to choose fast growth, mycelia is fine and close, pure color
Bacterium colony be transferred on slant medium.
Present invention white beche-de-mer is as selenium source, it is to avoid add the insecurity of sodium selenite, the Cordyceps sporophore turned out
Not only the content of selenium element is high, moreover it is possible to make rich in protein in Stichopus japonicus, aminoacid and other trace element as the battalion of Cordyceps
The source of supporting, makes the Cordyceps medical value turned out higher.Sporophore is sturdy, and bright in colour, Se content can reach 117 micro-gram gram, worm
Grass cellulose content can reach 4.3 milligrams per gram.
Below in conjunction with embodiment, the invention will be further described.
Detailed description of the invention
The cultural method of the Cordyceps sporophore of a kind of selenium-rich high cordycepin content, is characterized in that: comprise the following steps: successively
(1) activation of strain: Cordyceps militaris spawn is connected in the fluid medium of following components and activates and optimize: glucose
15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram, vitamin B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram,
Yeast 3-5 gram of powder of leaching, agar powder 15-20 gram, add water to 1000 milliliters；By aforesaid liquid culture medium at 121 DEG C, 0.1MPa condition
Lower sterilizing 30 minutes, pours in culture dish after cooling；Cordyceps militaris spawn is accessed in culture dish, cultivate under 22 DEG C of dark conditions
5-10 days, bacterium colony was transferred on slant medium again, cultivated 5-10 days under 22 DEG C of dark conditions, the most required Cordyceps of gained strain
Kind；
(2) preparation of liquid spawn:
First preparing nutritional solution A: glucose 15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram by following formula, dimension is raw
Element B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram, yeast 3-5 gram of powder of leaching, add water to 1000 milliliters；Nutritional solution A is divided
Being contained in the conical flask of 500 milliliters, 250 milliliters every bottle, sealed membrane seals, at 121 DEG C, under the conditions of 0.1MPa, and sterilizing 30 minutes,
On super-clean bench, access the cordyceps species activated through step (1) after cooling, then at 130 revs/min, the shaking table of 22 DEG C shakes
Cultivate 5-9 days；Treat liquid occurs uniform fungus ball and more thick available.
(3) rice medium is prepared:
First configure nutritional solution B: glucose 15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram, vitamin by formula as below
B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram, yeast 3-5 gram of powder of leaching, add water to 1000 milliliters；
The preparation of rice medium: with the wide-mouth culture bottle of 350 milliliters, every bottle in following ratio loading compost: rice 35-40
Gram, nutritional solution B 40 ~ 50 milliliters, cover tightly with the lid of band passage or tighten culture bottle bottleneck with polyethylene plastic film；
(4) sterilizing: culture bottle is placed on 121 DEG C, under the conditions of 0.1MPa, sterilizing 30 minutes；
(5) inoculation: aseptically, each culture bottle accesses 5 milliliters of liquid strains, sealing；
(6) mycelial cultivation: postvaccinal culture bottle is moved under dark condition and cultivate；Start temperature and keep 16 DEG C ~ 18 DEG C
4 ~ 6 days；It is further cultured under the conditions of temperature being risen to 20 DEG C ~ 24 DEG C after culture bottle surface covers with white hypha 4 ~ 6 days, treats that mycelia eats
Thoroughly after culture medium, move on to culture bottle cultivate under light；
(7) annesl: keep indoor temperature 20 DEG C ~ 22 DEG C, humidity 60% ~ 70%, intensity of illumination 800 lux, light application time every day
12 ~ 14 hours；After being cultivated 3 ~ 5 days by culture bottle, visible mycelia is gradually transferred to crocus by white, is down to by nocturnal temperature afterwards
10 DEG C ~ 15 DEG C temperature difference keeping having 5 ~ 10 DEG C round the clock, when hyphal surface is covered with the projection of grain of rice size, stop day and night temperature and turn
For constant temperature culture；
(8) cultivation of sporophore: holding indoor temperature 20 DEG C ~ 22 DEG C, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 lux,
12 ~ 14 hours every days of light application time, indoor humidity is brought up to 75% ~ 85%, temperature and illumination after sporophore grows to 3 ~ 4 centimetres
Time is constant；
(9) gather: when sporophore grows to 6 ~ 8 centimetres, with tweezers, sporophore is pressed from both sides out from culture bottle, be placed at aeration-drying
Natural air drying or 65 DEG C of baking ovens are dried, dry sporophore is contained in airtight plastic bag and keeps in Dark Place.
When in step (1), bacterium colony is transferred on slant medium, being to choose fast growth, mycelia is fine and close, pure color
Bacterium colony be transferred on slant medium.
The making of white beche-de-mer lapping liquid: fresh white beche-de-mer is gilled or white beche-de-mer that steep raising is good is put in cooking machine
Break into homogenate.
Although the white beche-de-mer of selenium-rich is precious rare, but has been able to scale artificial culture at present, production cost is the highest.
Claims (2)
1. a cultural method for the Cordyceps sporophore of selenium-rich high cordycepin content, is characterized in that: comprise the following steps: successively
(1) activation of strain: Cordyceps militaris spawn is connected in the fluid medium of following components and activates and optimize: glucose
15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram, vitamin B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram,
Yeast 3-5 gram of powder of leaching, agar powder 15-20 gram, add water to 1000 milliliters；By aforesaid liquid culture medium at 121 DEG C, 0.1MPa condition
Lower sterilizing 30 minutes, pours in culture dish after cooling；Cordyceps militaris spawn is accessed in culture dish, cultivate under 22 DEG C of dark conditions
5-10 days, bacterium colony was transferred on slant medium again, cultivated 5-10 days under 22 DEG C of dark conditions, the most required Cordyceps of gained strain
Kind；
(2) preparation of liquid spawn:
First preparing nutritional solution A: glucose 15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram by following formula, dimension is raw
Element B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram, yeast 3-5 gram of powder of leaching, add water to 1000 milliliters；Nutritional solution A is divided
Being contained in the conical flask of 500 milliliters, 250 milliliters every bottle, sealed membrane seals, at 121 DEG C, under the conditions of 0.1MPa, and sterilizing 30 minutes,
On super-clean bench, access the cordyceps species activated through step (1) after cooling, then at 130 revs/min, the shaking table of 22 DEG C shakes
Cultivate 5-9 days；
(3) rice medium is prepared:
First configure nutritional solution B: glucose 15-20 gram, 1-2 gram of magnesium sulfate, potassium dihydrogen phosphate 1-2 gram, vitamin by formula as below
B1 0.1-0.5 milligram, white beche-de-mer lapping liquid 45-55 gram, yeast 3-5 gram of powder of leaching, add water to 1000 milliliters；
The preparation of rice medium: with the wide-mouth culture bottle of 350 milliliters, every bottle in following ratio loading compost: rice 35-40
Gram, nutritional solution B 40 ~ 50 milliliters, cover tightly with the lid of band passage or tighten culture bottle bottleneck with polyethylene plastic film；
(4) sterilizing: culture bottle is placed on 121 DEG C, under the conditions of 0.1MPa, sterilizing 30 minutes；
(5) inoculation: aseptically, each culture bottle accesses 5 milliliters of liquid strains, sealing；
(6) mycelial cultivation: postvaccinal culture bottle is moved under dark condition and cultivate；Start temperature and keep 16 DEG C ~ 18 DEG C
4 ~ 6 days；It is further cultured under the conditions of temperature being risen to 20 DEG C ~ 24 DEG C after culture bottle surface covers with white hypha 4 ~ 6 days, treats that mycelia eats
Thoroughly after culture medium, move on to culture bottle cultivate under light；
(7) annesl: keep indoor temperature 20 DEG C ~ 22 DEG C, humidity 60% ~ 70%, intensity of illumination 800 lux, light application time every day
12 ~ 14 hours；After being cultivated 3 ~ 5 days by culture bottle, visible mycelia is gradually transferred to crocus by white, is down to by nocturnal temperature afterwards
10 DEG C ~ 15 DEG C temperature difference keeping having 5 ~ 10 DEG C round the clock, when hyphal surface is covered with the projection of grain of rice size, stop day and night temperature and turn
For constant temperature culture；
(8) cultivation of sporophore: holding indoor temperature 20 DEG C ~ 22 DEG C, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 lux,
12 ~ 14 hours every days of light application time, indoor humidity is brought up to 75% ~ 85%, temperature and illumination after sporophore grows to 3 ~ 4 centimetres
Time is constant；
(9) gather: when sporophore grows to 6 ~ 8 centimetres, with tweezers, sporophore is pressed from both sides out from culture bottle, be placed at aeration-drying
Natural air drying or 65 DEG C of baking ovens are dried, dry sporophore is contained in airtight plastic bag and keeps in Dark Place.
The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content the most according to claim 1, is characterized in that: step
(1) when in, bacterium colony is transferred on slant medium, being to choose fast growth, mycelia is fine and close, and the bacterium colony of pure color is transferred to
On slant medium.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108464186A (en) * | 2018-03-22 | 2018-08-31 | 即墨市高淑娥家庭农场 | The method and products thereof of Cordceps militaris is cultivated using sea cucumber and clam |
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| CN103858673A (en) * | 2014-03-18 | 2014-06-18 | 鲁东大学 | Method for planting cordyceps militaris by beer residue |
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| CN104584858A (en) * | 2015-01-05 | 2015-05-06 | 鲁东大学 | Method for planting cordyceps militaris by taking wooden shaving as carrier |
| CN104871830A (en) * | 2015-06-15 | 2015-09-02 | 鲁东大学 | Method for planting cordyceps militaris with bean worms as carriers |
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2016
- 2016-06-12 CN CN201610404341.4A patent/CN106069177A/en active Pending
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| CN103011953A (en) * | 2012-12-05 | 2013-04-03 | 青岛艾华隆生物科技有限公司 | Culture medium for increasing edible fungus polysaccharide content and preparation method thereof |
| CN103858673A (en) * | 2014-03-18 | 2014-06-18 | 鲁东大学 | Method for planting cordyceps militaris by beer residue |
| CN104396571A (en) * | 2014-12-15 | 2015-03-11 | 重庆综艺制药有限公司 | High-cordycepin-content rich-selenium cordyceps sinensis cultivation method |
| CN104584858A (en) * | 2015-01-05 | 2015-05-06 | 鲁东大学 | Method for planting cordyceps militaris by taking wooden shaving as carrier |
| CN104871830A (en) * | 2015-06-15 | 2015-09-02 | 鲁东大学 | Method for planting cordyceps militaris with bean worms as carriers |
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| CN108464186A (en) * | 2018-03-22 | 2018-08-31 | 即墨市高淑娥家庭农场 | The method and products thereof of Cordceps militaris is cultivated using sea cucumber and clam |
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Application publication date: 20161109 |