CN103858673B - Method for planting cordyceps militaris by beer residue - Google Patents
Method for planting cordyceps militaris by beer residue Download PDFInfo
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- CN103858673B CN103858673B CN201410098406.8A CN201410098406A CN103858673B CN 103858673 B CN103858673 B CN 103858673B CN 201410098406 A CN201410098406 A CN 201410098406A CN 103858673 B CN103858673 B CN 103858673B
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Abstract
The invention discloses a method for planting cordyceps militaris by beer residue. The method comprises the steps of beer residue drying, liquid strain preparation, nutrition liquid preparation, sterilization, inoculation, dark culture, color changing, fruit body growing, harvesting and the like. The method has the advantages that the beer residue is successfully utilized for planting the cordyceps militaris, and the biotransformation rate of the beer residue is greatly improved, so nutrition substances in the beer residue are utilized to the maximum degree, and the effects of no pollution and no waste in the production process are really realized.
Description
Technical field
The present invention relates to a kind of method of planting Cordyceps militaris (L.) Link..
Background technology
Beer residue is the principal by product of brewing industry, is take barley as raw material, the residue after soluble-carbohydrate in fermented extracted seed.The main component of beer residue is malt husks and residual starch, protein, amino acid and trace element etc., after the series of process process such as dehydration, fermentation, can obtain from beer residue and reach 50% to 70% malt albumen containing protein, be used for cultivation aspect at present, can directly be used for keeping fowls and pig, ox, sheep, etc. domestic animal.Along with Economic development and people's living standard improve, also increase year by year beer consumption, as you know, China has been current first beer production state in the world, therefore, while production beer, also creates a large amount of beer residue.How better this nutritious industrial residue of development and utilization, become the problem that everybody falls over each other to study.
Summary of the invention
The object of the present invention is to provide a kind of biological transformation ratio improving beer residue, the method with the beer residue plantation Cordyceps militaris (L.) Link. nutritive substance in beer residue being obtained utilize to greatest extent.
Technical solution of the present invention is:
A method for beer residue plantation Cordyceps militaris (L.) Link., is characterized in that: comprise the following steps:
(1) oven dry of beer residue: beer residue to be placed in the baking oven of 60 DEG C drying and processing 1.5 ~ 2.5 hours;
(2) preparation of liquid spawn: the liquid nutrient medium prepared: the recipe ingredient of liquid nutrient medium is by weight: glucose 10 ~ 30 grams, potassium primary phosphate 1 ~ 5 gram, 1 ~ 5 gram, magnesium sulfate, peptone 5 ~ 15 grams, vitamins B 150 milligrams, 1000 milliliters, water; The liquid nutrient medium prepared is sub-packed in Erlenmeyer flask, 250 milliliters every bottle, sealing, sterilizing 25 ~ 35 minutes under 120 DEG C ~ 130 DEG C conditions; Take out substratum after sterilizing, naturally cool to room temperature, aseptically access Cordyceps militaris spawn; At 15 DEG C ~ 25 DEG C, shake cultivation in the shaking table of 110 ~ 140 revs/min 4 ~ 10 days;
(3) preparation of nutritive medium: the recipe ingredient of nutritive medium is by weight: glucose 25 ~ 40 grams, potassium primary phosphate 1 ~ 5 gram, 1 ~ 5 gram, magnesium sulfate, peptone 15 ~ 25 grams, vitamins B 150 milligrams, 1000 milliliters, water;
(4) sterilizing: with the wide-necked bottle of 500 milliliters, every bottle loads culture material in following ratio: the dry beer schlempe that step (1) obtains 30 grams, Semen Maydis powder 10 grams, nutritive medium 40 ~ 50 milliliters prepared by step (3), cover tightly with the lid of band ventilating pit or tighten bottleneck with polyethylene plastic film, bottle to be placed under 120 DEG C ~ 130 DEG C conditions sterilizing 25 ~ 35 minutes, to take out to be placed on Bechtop and be cooled to room temperature;
(5) inoculate: aseptically every bottle graft enters liquid spawn, sealing;
(6) dark culturing: cultivate under postvaccinal culturing bottle is moved into dark condition, start temperature keep 18 DEG C ~ 20 DEG C 4 ~ 6 days, until culturing bottle surface cover with after white hypha, temperature to be risen to 20 DEG C ~ 24 DEG C conditions under cultivate 8 ~ 12 days again, until mycelia have thorough grasp after substratum, culturing bottle to be moved on to light under cultivate;
(7) annesl: keep room temp 20 DEG C ~ 24 DEG C, humidity 60% ~ 70%, intensity of illumination 800 lux, 12 ~ 14 hours every days of light application time, cultivate mycelia after 5 ~ 8 days and transfer safran to gradually by white, nocturnal temperature is down to 10 DEG C ~ 15 DEG C afterwards, keep the temperature difference having 5 ~ 10 DEG C round the clock, when hyphal surface is covered with the projection of grain of rice size, day and night temperature is stopped to transfer 20 DEG C ~ 24 DEG C constant temperature culture to;
(8) growth of sporophore: keep room temp 20 DEG C ~ 24 DEG C, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 lux, 12 ~ 14 hours every days of light application time, after sporophore grows to 3 ~ 4 centimetres, indoor humidity is brought up to 75% ~ 85%, temperature and light is constant according to the time;
(9) gather: when sporophore grows to 6 ~ 8 centimetres, sporophore is taken out from culturing bottle, be placed in air seasoning place natural air drying or 60 DEG C of baking ovens and dry, be contained in airtight plastics bag and keep in Dark Place.
Beer residue in step (1) is young beer slag.
In step (5), the access amount of liquid spawn is 5 milliliters.
The present invention successfully utilizes beer residue to plant out Cordyceps militaris (L.) Link., substantially increase the biological transformation ratio of beer residue, nutritive substance in beer residue is obtained utilize to greatest extent, and the cordycepic acid content in culture medium of Cordyceps militaris is 3.95%, cordycepin 0.78% and Cordyceps polysaccharide 11.8%, can be pulverized as fodder additives, greatly be improved the immunological competence of domestic birds and animals.Really accomplish that production process is pollution-free, without waste.
Below in conjunction with embodiment, the invention will be further described.
A method for beer residue plantation Cordyceps militaris (L.) Link., is characterized in that: comprise the following steps:
(1) oven dry of beer residue: young beer slag to be placed in the baking oven of 60 DEG C drying and processing 1.5 ~ 2.5 hours;
(2) preparation of liquid spawn: the liquid nutrient medium prepared: the recipe ingredient of liquid nutrient medium is by weight: glucose 10 ~ 30 grams, potassium primary phosphate 1 ~ 5 gram, 1 ~ 5 gram, magnesium sulfate, peptone 5 ~ 15 grams, vitamins B 150 milligrams, 1000 milliliters, water; The liquid nutrient medium prepared is sub-packed in Erlenmeyer flask, 250 milliliters every bottle, sealing, sterilizing 25 ~ 35 minutes under 120 DEG C ~ 130 DEG C conditions; Take out substratum after sterilizing, naturally cool to room temperature, aseptically access Cordyceps militaris spawn; At 15 DEG C ~ 25 DEG C, shake cultivation in the shaking table of 110 ~ 140 revs/min 4 ~ 10 days;
(3) preparation of nutritive medium: the recipe ingredient of nutritive medium is by weight: glucose 25 ~ 40 grams, potassium primary phosphate 1 ~ 5 gram, 1 ~ 5 gram, magnesium sulfate, peptone 15 ~ 25 grams, vitamins B 150 milligrams, 1000 milliliters, water;
(4) sterilizing: with the wide-necked bottle of 350 milliliters, every bottle loads culture material in following ratio: the dry beer schlempe that step (1) obtains 30 grams, Semen Maydis powder 10 grams, nutritive medium 40 ~ 50 milliliters prepared by step (3), cover tightly with the lid of band ventilating pit or tighten bottleneck with polyethylene plastic film, bottle to be placed under 120 DEG C ~ 130 DEG C conditions sterilizing 25 ~ 35 minutes, to take out to be placed on Bechtop and be cooled to room temperature;
(5) inoculate: aseptically every bottle graft enters 5 milliliters of liquid bacterial classifications, sealing;
(6) dark culturing: cultivate under postvaccinal culturing bottle is moved into dark condition, start temperature keep 18 DEG C ~ 20 DEG C 4 ~ 6 days, until culturing bottle surface cover with after white hypha, temperature to be risen to 20 DEG C ~ 24 DEG C conditions under cultivate 8 ~ 12 days again, until mycelia have thorough grasp after substratum, culturing bottle to be moved on to light under cultivate;
(7) annesl: keep room temp 20 DEG C ~ 24 DEG C, humidity 60% ~ 70%, intensity of illumination 800 lux, 12 ~ 14 hours every days of light application time, cultivate mycelia after 5 ~ 8 days and transfer safran to gradually by white, nocturnal temperature is down to 10 DEG C ~ 15 DEG C afterwards, keep the temperature difference having 5 ~ 10 DEG C round the clock, when hyphal surface is covered with the projection of grain of rice size, day and night temperature is stopped to transfer 20 DEG C ~ 24 DEG C constant temperature culture to;
(8) growth of sporophore: keep room temp 20 DEG C ~ 24 DEG C, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 lux, 12 ~ 14 hours every days of light application time, after sporophore grows to 3 ~ 4 centimetres, indoor humidity is brought up to 75% ~ 85%, temperature and light is constant according to the time;
(9) gather: when sporophore grows to 6 ~ 8 centimetres, with tweezers, sporophore is pressed from both sides out from culturing bottle, be placed in air seasoning place natural air drying or 60 DEG C of baking ovens and dry, be contained in airtight plastics bag and keep in Dark Place.
(10) after substratum being dried pulverizing, can as feed additive production animal feed.
Claims (3)
1., by a method for beer residue plantation Cordyceps militaris (L.) Link., it is characterized in that: comprise the following steps:
(1) oven dry of beer residue: beer residue to be placed in the baking oven of 60 DEG C drying and processing 1.5 ~ 2.5 hours;
(2) preparation of liquid spawn: the liquid nutrient medium prepared: the recipe ingredient of liquid nutrient medium is by weight: glucose 10 ~ 30 grams, potassium primary phosphate 1 ~ 5 gram, 1 ~ 5 gram, magnesium sulfate, peptone 5 ~ 15 grams, vitamins B 150 milligrams, 1000 milliliters, water; The liquid nutrient medium prepared is sub-packed in Erlenmeyer flask, 250 milliliters every bottle, sealing, sterilizing 25 ~ 35 minutes under 120 DEG C ~ 130 DEG C conditions; Take out substratum after sterilizing, naturally cool to room temperature, aseptically access Cordyceps militaris spawn; At 15 DEG C ~ 25 DEG C, shake cultivation in the shaking table of 110 ~ 140 revs/min 4 ~ 10 days;
(3) preparation of nutritive medium: the recipe ingredient of nutritive medium is by weight: glucose 25 ~ 40 grams, potassium primary phosphate 1 ~ 5 gram, 1 ~ 5 gram, magnesium sulfate, peptone 15 ~ 25 grams, vitamins B 150 milligrams, 1000 milliliters, water;
(4) sterilizing: with the wide-necked bottle of 500 milliliters, every bottle loads culture material in following ratio: the dry beer schlempe that step (1) obtains 30 grams, Semen Maydis powder 10 grams, nutritive medium 40 ~ 50 milliliters prepared by step (3), cover tightly with the lid of band ventilating pit or tighten bottleneck with polyethylene plastic film, bottle to be placed under 120 DEG C ~ 130 DEG C conditions sterilizing 25 ~ 35 minutes, to take out to be placed on Bechtop and be cooled to room temperature;
(5) inoculate: aseptically every bottle graft enters liquid spawn, sealing;
(6) dark culturing: cultivate under postvaccinal culturing bottle is moved into dark condition, start temperature keep 18 DEG C ~ 20 DEG C 4 ~ 6 days, until culturing bottle surface cover with after white hypha, temperature to be risen to 20 DEG C ~ 24 DEG C conditions under cultivate 8 ~ 12 days again, until mycelia have thorough grasp after substratum, culturing bottle to be moved on to light under cultivate;
(7) annesl: keep room temp 20 DEG C ~ 24 DEG C, humidity 60% ~ 70%, intensity of illumination 800 lux, 12 ~ 14 hours every days of light application time, cultivate mycelia after 5 ~ 8 days and transfer safran to gradually by white, nocturnal temperature is down to 10 DEG C ~ 15 DEG C afterwards, keep the temperature difference having 5 ~ 10 DEG C round the clock, when hyphal surface is covered with the projection of grain of rice size, day and night temperature is stopped to transfer 20 DEG C ~ 24 DEG C constant temperature culture to;
(8) growth of sporophore: keep room temp 20 DEG C ~ 24 DEG C, humidity 60% ~ 70%, intensity of illumination 600 ~ 800 lux, 12 ~ 14 hours every days of light application time, after sporophore grows to 3 ~ 4 centimetres, indoor humidity is brought up to 75% ~ 85%, temperature and light is constant according to the time;
(9) gather: when sporophore grows to 6 ~ 8 centimetres, sporophore is taken out from culturing bottle, be placed in air seasoning place natural air drying or 60 DEG C of baking ovens and dry, be contained in airtight plastics bag and keep in Dark Place.
2. the method for beer residue plantation Cordyceps militaris (L.) Link. according to claim 1, is characterized in that: the beer residue in step (1) is young beer slag.
3. the method for beer residue plantation Cordyceps militaris (L.) Link. according to claim 1, is characterized in that: in step (5), the access amount of liquid spawn is 5 milliliters.
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| CN201410098406.8A CN103858673B (en) | 2014-03-18 | 2014-03-18 | Method for planting cordyceps militaris by beer residue |
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| CN103858673B true CN103858673B (en) | 2015-04-08 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104498367B (en) * | 2014-11-28 | 2017-06-20 | 沈阳源康生物技术发展有限公司 | A kind of culture medium and its application method for Cordyceps militaris submerged fermentation |
| CN105779300A (en) * | 2016-02-22 | 2016-07-20 | 嘉兴市潜福食品有限公司 | Solid culture medium and cultivation method of cordyceps militaris fruiting bodies |
| CN105941628A (en) * | 2016-05-03 | 2016-09-21 | 内蒙古鼎祥健康产业投资有限公司 | Preparation method of health-care Chinese caterpillar fungus peptide milk for middle-aged and aged people |
| CN106069177A (en) * | 2016-06-12 | 2016-11-09 | 鲁东大学 | The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content |
| CN107114119B (en) * | 2017-05-20 | 2018-09-07 | 天津市东方中滨农业科技有限公司 | A method of Cordyceps militaris is cultivated using thermal stimulation |
| CN108812050A (en) * | 2018-05-11 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving Cordyceps militaris yield |
| CN108633616A (en) * | 2018-05-11 | 2018-10-12 | 江苏圣福来生态农业有限公司 | A kind of culture implantation methods improving activity substance content in Cordyceps militaris |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946638A (en) * | 2010-09-20 | 2011-01-19 | 福建省曲斗香酒业有限公司 | Method for culturing cordyceps militaris by distilled grain |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946638A (en) * | 2010-09-20 | 2011-01-19 | 福建省曲斗香酒业有限公司 | Method for culturing cordyceps militaris by distilled grain |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
Non-Patent Citations (1)
| Title |
|---|
| 蛹虫草菌丝固体法培养初探;徐冲等;《山东农业科学》;20101231(第11期);66-68 * |
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