CN103011953A - Culture medium for increasing edible fungus polysaccharide content and preparation method thereof - Google Patents
Culture medium for increasing edible fungus polysaccharide content and preparation method thereof Download PDFInfo
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- CN103011953A CN103011953A CN 201210513291 CN201210513291A CN103011953A CN 103011953 A CN103011953 A CN 103011953A CN 201210513291 CN201210513291 CN 201210513291 CN 201210513291 A CN201210513291 A CN 201210513291A CN 103011953 A CN103011953 A CN 103011953A
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Abstract
The invention relates to a culture medium for increasing edible fungus polysaccharide content and a preparation method thereof. The raw materials include sawdust, wheat bran, corn flour, lime and peanut shell powder; the culture medium is characterized by comprising sea cucumber cooking liquor enzymolysis dry powder; and the preparation method comprises the enzymolysis of the sea cucumber cooking liquor. By the culture medium and the preparation method, an excellent culture medium is made from the sea cucumber cooking liquor dry powder to culture edible fungus, so that the edible fungus with the maximum polysaccharide content of 18.5% can be obtained, the polysaccharide content of the edible fungus is greatly increased; and meanwhile, the recycling of the sea cucumber cooking liquor is beneficial to environmental friendliness and can be used for turning waste into valuables, and the social economic benefits are realized to the greatest extent.
Description
Technical field
The present invention relates to a kind of cultivating method for edible fungi, especially a kind of substratum that improves edible fungi polysaccharide content and preparation method thereof.
Background technology
The domestic fungus resource of China is abundant, also is one of country of the earliest culturing edible fungus.Edible mushrooms is not only delicious, and nutritious, often is called heath food by people, the amino acid that not only contains various needed by human such as mushroom, also contain mineral element and the multivitamins such as calcium, iron, phosphorus, to promoting human body metabolism, improving organism adaptation power has great role.Especially Recent study finds that lentinan can improve the vigor of complementary T cell, reduces the ability of methyl-cholanthrene induced tumor, simultaneously cancer cells is had strong restraining effect; Experiment shows that lentinan is 97.5% to the inhibiting rate of small white mouse sarcoma 180, is 80% to the inhibiting rate of ehrlich carcinoma.But because the polysaccharide content of edible mushrooms all hangs down below 10%, the polysaccharide content that therefore improves edible mushrooms is the focus of present cultivation technique.
Along with the development of human sciences's technology, the cultivation technique of edible mushrooms is also at development, and wherein the development of substratum is vital.Contain a large amount of polysaccharide in the sea cucumber cooking waste liquor, if this is applied in the culture medium of edible fungus, not only be conducive to improve the nutritive value of edible mushrooms, can also turn waste into wealth, environment protection reduces and pollutes.
Summary of the invention
The object of the invention is to overcome the shortcoming that above-mentioned prior art exists, a kind of substratum that can improve edible fungi polysaccharide content and preparation method thereof is provided.
A kind of substratum that can improve edible fungi polysaccharide content of the present invention comprises wood chip, wheat bran, Semen Maydis powder, lime, peanut hull meal, and its invention main points are that it also contains sea cucumber cooking liquor enzymolysis dry powder; Its preparation method is that above-mentioned batching is mixed, stirs, pulverized, and makes it even, refinement, carries out at last high-temperature sterilization.
Described sea cucumber cooking liquor is the liquid that sea cucumber discards in the boiling course of processing, and contains a large amount of nutritive substances in the sea cucumber cooking liquor, if arbitrarily discharging not only can cause environmental pollution, has also caused the wasting of resources simultaneously.Therefore the inventor carries out the sea cucumber cooking liquor to be applied in the substratum of edible mushrooms behind the polysaccharide enzymolysis, and it is utilized effectively, and has also improved the polysaccharide content of edible mushrooms simultaneously.
The production technique of sea cucumber cooking liquor enzymolysis dry powder is, the sea cucumber cooking liquor is concentrated, oven dry, and 1:20 adds polysaccharide hydrolase in mass ratio, 60 ℃ of hydrolysis temperatures, then enzymolysis time 2 days gets final product the enzymolysis solution concentrate drying.
The prescription of substratum is: 10% ~ 30% sea cucumber cooking liquor enzymolysis dry powder, 65% ~ 45% wood chip, 10% wheat bran, 10% peanut hull meal, 4% Semen Maydis powder, 1% lime.
The technique of high-temperature sterilization: pressure is that 0.15MPa, temperature are 120 ℃, and sterilization time is 60 minutes.The purpose of high-temperature sterilization is parasite and the miscellaneous bacteria of killing in the batching, avoids polluting or disease and pest.Substratum behind the high-temperature sterilization can be directly used in inoculation, the cultivation of edible mushrooms.
The invention has the advantages that the waste that has not only solved the sea cucumber cooking liquor, protection of the environment, and after being applied to the sea cucumber cooking liquor in the culture medium of edible fungus, the polysaccharide content of edible mushrooms is increased to 16% ~ 18.5% from 7% ~ 8%, has increased more than 1 times.
Embodiment
Embodiment 1, and a kind of substratum that improves edible fungi polysaccharide content and preparation method thereof may further comprise the steps:
(1) take sea cucumber cooking liquor enzymolysis dry powder, wood chip, wheat bran, Semen Maydis powder, lime and peanut hull meal as feed proportioning;
(2) will prepare burden mixing, stirring, pulverize, make it evenly, refinement;
(3) will be evenly, the raw material high-temperature sterilization after the refinement.
The quality prescription of raw material is: 10% sea cucumber cooking liquor enzymolysis dry powder, 65% wood chip, 10% wheat bran, 10% peanut hull meal, 4% Semen Maydis powder, 1% lime add water by 70% of above-mentioned total mass.
The technique of high-temperature sterilization wherein: pressure is that 0.15MPa, temperature are 120 ℃, and sterilization time is 60 minutes.
The contained polysaccharide content of common edible mushrooms is 7% ~ 8%, and is 16% with the edible fungi polysaccharide content that above-mentioned substratum cultivates.
Embodiment 2, a kind of substratum that improves edible fungi polysaccharide content and preparation method thereof, the quality prescription of its raw material is: 20% sea cucumber cooking liquor enzymolysis dry powder, 55% wood chip, 10% wheat bran, 10% peanut hull meal, 4% Semen Maydis powder, 1% lime add water by 70% of above-mentioned total mass.All the other are with embodiment 1.The edible fungi polysaccharide content that cultivates with above-mentioned substratum is 18.5%.
Embodiment 3, a kind of substratum that improves edible fungi polysaccharide content and preparation method thereof, the quality prescription of its raw material is: 30% sea cucumber cooking liquor enzymolysis dry powder, 45% wood chip, 10% wheat bran, 10% peanut hull meal, 4% Semen Maydis powder, 1% lime add water by 70% of above-mentioned total mass.All the other are with embodiment 1.The edible fungi polysaccharide content that cultivates with above-mentioned substratum is 17.5%.
Above-described embodiment is not as a limitation of the invention, all any modifications of doing within the scope of the invention, is equal to replacement, improvement etc., all belongs to protection scope of the present invention.
Claims (4)
1. substratum that improves edible fungi polysaccharide content and preparation method thereof is characterized in that: this substratum adopts following methods preparation:
(1) take sea cucumber cooking liquor enzymolysis dry powder, wood chip, wheat bran, Semen Maydis powder, lime and peanut hull meal as feed proportioning;
(2) will prepare burden mixing, stirring, pulverize, make it evenly, refinement;
(3) will be evenly, the raw material high-temperature sterilization after the refinement.
2. according to substratum of raising edible fungi polysaccharide content claimed in claim 1 and preparation method thereof, it is characterized in that: the quality prescription of raw material is: 10% ~ 30% sea cucumber cooking liquor enzymolysis dry powder, 65% ~ 45% wood chip, 10% wheat bran, 10% peanut hull meal, 4% Semen Maydis powder, 1% lime.
3. according to substratum of raising edible fungi polysaccharide content claimed in claim 1 and preparation method thereof, it is characterized in that: the production technique of sea cucumber cooking liquor enzymolysis dry powder is, the sea cucumber cooking liquor is concentrated, oven dry, 1:20 adds polysaccharide hydrolase in mass ratio, 60 ℃ of hydrolysis temperatures, then enzymolysis time 2 days gets final product the enzymolysis solution concentrate drying.
4. according to substratum of raising edible fungi polysaccharide content claimed in claim 1 and preparation method thereof, it is characterized in that: the pressure of high-temperature sterilization is that 0.15MPa, temperature are 120 ℃, and the time is 60 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210513291 CN103011953A (en) | 2012-12-05 | 2012-12-05 | Culture medium for increasing edible fungus polysaccharide content and preparation method thereof |
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|---|---|---|---|
| CN 201210513291 CN103011953A (en) | 2012-12-05 | 2012-12-05 | Culture medium for increasing edible fungus polysaccharide content and preparation method thereof |
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| CN103011953A true CN103011953A (en) | 2013-04-03 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288529A (en) * | 2013-04-16 | 2013-09-11 | 安徽农业大学 | Mushroom culture material and preparation method thereof |
| CN105110978A (en) * | 2015-09-18 | 2015-12-02 | 临汾市尧都区杜怡霖种植专业合作社 | Cultivation medium used for increasing lentinan content in lentinus edodes |
| CN106069177A (en) * | 2016-06-12 | 2016-11-09 | 鲁东大学 | The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content |
-
2012
- 2012-12-05 CN CN 201210513291 patent/CN103011953A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288529A (en) * | 2013-04-16 | 2013-09-11 | 安徽农业大学 | Mushroom culture material and preparation method thereof |
| CN103288529B (en) * | 2013-04-16 | 2015-01-21 | 安徽农业大学 | Mushroom culture material and preparation method thereof |
| CN105110978A (en) * | 2015-09-18 | 2015-12-02 | 临汾市尧都区杜怡霖种植专业合作社 | Cultivation medium used for increasing lentinan content in lentinus edodes |
| CN106069177A (en) * | 2016-06-12 | 2016-11-09 | 鲁东大学 | The cultural method of the Cordyceps sporophore of selenium-rich high cordycepin content |
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Application publication date: 20130403 |