CN101849477A - Cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as host and cultivating method thereof - Google Patents
Cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as host and cultivating method thereof Download PDFInfo
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- CN101849477A CN101849477A CN200910113947A CN200910113947A CN101849477A CN 101849477 A CN101849477 A CN 101849477A CN 200910113947 A CN200910113947 A CN 200910113947A CN 200910113947 A CN200910113947 A CN 200910113947A CN 101849477 A CN101849477 A CN 101849477A
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Abstract
The invention provides a cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as a host and a cultivating method thereof. The cassava silkworm coydyceps militaris (L.) link is specifically characterized in that: the cassava silkworm chrysalis is yellow brown or dark brown mycelium with a slightly yellow body surface; and rod-like or branched orange-yellow fruiting bodies are grown on the body surface of the silkworm chrysalis. The cassava silkworm coydyceps militaris (L.) link is cultivated by the following steps of: cultivating the strain of the coydyceps militaris (L.) link into liquid strain of the coydyceps militaris (L.) link; injecting the strain into the body of the cassava silkworm chrysalis by using a sterilized syringe; and then controlling the environment conditions such as the temperature, the moisture, the light and the like to cultivate the cassava silkworm coydyceps militaris (L.) link. The cassava silkworm coydyceps militaris (L.) link product has various active ingredients and nutritional ingredients, has a similar function to that of cordyceps sinensis, and can be used as a medicament, a health-care product and food; and the cultivating method is simple, has short period and low cost and is a practical method for developing the novel coydyceps militaris.
Description
Technical field
The present invention relates to cultivation cassava silkworm Cordyceps militaris and breeding method thereof in a kind of biological technical field.
Background technology
Cordyceps militaris Coydyceps militaris (L.) Link is called northern Chinese caterpillar Fungus again, the pupa grass, and Cordyceps militaris etc. belong to Ascomycota, Ascomycetes, Hypocreales, Clavicipitaceae, Chinese caterpillar fungus Pseudomonas.Cordyceps militaris is pupa or the larva of occurring in nature main parasitic in lepidopterous insects, and Cordyceps militaris all has distribution in the whole world, and all there is distribution China each province.
To the Cordyceps sinensis that Cordyceps militaris belongs to together very similar medicinal and health-care effect is arranged, as regulate immunity, antifatigue, anti-ageing, anti-inflammatory, antiviral, particularly tumour is all had stronger inhibitory action.Cordyceps militaris is subjected to consumer's favor with its abundant nutrition and unique medical value, and the market demand is vigorous.Wild Chinese caterpillar fungus is fewer and feweri now, is difficult to meet the need of market.Therefore cassava silkworm chrysalis artificial cultivation Cordyceps militaris is the optimal path that solves this contradiction.
At present except utilizing rice etc. to do the medium culture fruiting bodies of cordyceps militaris, also by being that the host cultivates Cordyceps militaris with the insect.As utilize mulberry silkworm chrysalis, and tussah chrysalis, grasshopper cicada pupa, yellow meal worm is cultivated Cordyceps militaris.
Cassava silkworm belongs to Lepidoptera, Saturniidae, formal name used at school Philosamia cynthiaricini Boisduval, with the cassava leaves is feed, and there are 1,000 ten thousand mu cassavas in Guangxi, accounts for 65% of the whole nation, also be that the maximum province of cassava silkworm is raised in the whole nation, the existing very long history of the edible in a large number cassava silkworm chrysalis of people from Guangxi.The moisture branch 70% of cassava silkworm chrysalis, protein content is 59% in the dry matter, fat content is 15%, sugar content is 6%, protein content is higher than mulberry silkworm chrysalis and tussah chrysalis, and the cassava silkworm chrysalis protein contains 18 seed amino acids, wherein contains the 46%-49% that essential amino acid accounts for total amount, be rich in trace element and little elements of giving birth to such as potassium, sodium, iron, selenium, zinc, magnesium. be a kind of extraordinary nutriment. therefore, the cassava silkworm Cordyceps militaris that utilizes the cassava silkworm chrysalis to cultivate will have wide development and application prospect.Do not utilize cordyceps to infect the research report that the cassava silkworm chrysalis is cultivated the cassava silkworm Cordyceps militaris but have as yet at present.
Summary of the invention:
The purpose of this invention is to provide a kind of is cassava silkworm Cordyceps militaris and the breeding method thereof that the host cultivates with the cassava silkworm chrysalis.
Cassava silkworm Cordyceps militaris provided by the present invention is with Cordyceps militaris Coydyceps militaris (L.) Link bacterial classification, and the cultivation of infection cassava silkworm chrysalis forms.
Cassava silkworm chrysalis provided by the present invention is a kind of new product for the Cordyceps militaris that the host cultivates, and following feature is arranged: its cassava silkworm pupal cell is yellowish-brown or sepia, and the mycelium that body surface is slightly yellow has clavate or multi-branched shape, orange-yellow fruit body in pupal cell table length.
Provided by the present invention is the breeding method of the cassava silkworm Cordyceps militaris cultivated of host with the cassava silkworm chrysalis, comprises inoculation, and stiff pupa is cultivated, and goes out incubation step such as grass cultivations grade:
Concrete cultivation following steps
(1) Cordyceps militaris (Coydyceps militaris (L.) Link) of adopting conventional method will gather or purchase to obtain through separation, purifying becomes Cordyceps militaris spawn, makes liquid spawn (this all is the conventional method of production liquid spawn) through the secondary expansion is numerous then.
(2) the cassava silk cocoon of having pupated, cut off the taking-up silkworm chrysalis.Then silkworm chrysalis was put into the disinfectant soaking disinfection made with ozone generator 5-10 minute,, injects 0.05-0.3ml in the cassava silkworm pupal cell of having sterilized with asepsis injector with the Cordyceps militaris liquid spawn that cultivates.
(3) the cassava silkworm chrysalis that infects being deployed in the pallet, is that 18-25 ℃ of relative air humidity is 60-70% in temperature, and under the dark condition, through cultivation in 10-15 days, the cassava silkworm chrysalis of infection was just stiff
(4) stiff cassava silkworm chrysalis is actual is that cordyceps utilizes that moisture, protein, fat, sugar etc. are nutriment in the silkworm chrysalis body, makes all to be full of mycelium in the silkworm chrysalis body.Stiff cassava silkworm chrysalis, put into the disinfectant soaking disinfection made with ozone generator 5-10 minute, put into the wide-mouth vial of 500ml then, seal with transparent membrane, in temperature is that 18-25 ℃ of relative air humidity is 90-100%, and intensity of illumination is to cultivate under the condition of 500-2000lux, through occurring cordycep militaris fruiting body entity primodium in 10-15 days, continue to cultivate 25-30 days, the oven dry of just can gathering when ascospore occurring etc. the fruit body head is stored.
Cassava silkworm Cordyceps militaris of the present invention (having pupa that grass is arranged) is through check and analysis, and its crude protein content is 61.63%, crude fat content is 12.74%, polyoses content is 17.22%, cordycepic acid (mannitol) content is 14.81%, cordycepin content is that 1.26mg/g, adenosine content are 0.92mg/g.
Its chemical composition of cassava silkworm Cordyceps militaris that the present invention produces is similar to Cordyceps sinensis, and effect is also similar to Cordyceps sinensis, therefore has wide commercial applications prospect.
The present invention utilizes the liquid Cordyceps militaris spawn, and the technology with injection system infects the cassava silkworm chrysalis makes cassava silkworm chrysalis infection rate reach 100%, and go out carelessness and reach more than 95%, be a kind of with short production cycle, production cost is low, practical approach simple to operate.
Embodiment:
Below in conjunction with embodiment the present invention is further specified.
(1) the cassava silkworm chrysalis is prepared
With the cassava silk cocoon of pupating 2 days, cut off silk cocoon with scissors, take out silkworm chrysalis, select not have the silkworm chrysalis of damaged health to be used to cultivate Cordyceps militaris.
(2) bacterial classification is prepared
The Cordyceps militaris (Coydyceps militaris (L.) Link) of gathering or buying obtains is become Cordyceps militaris spawn through separation, purifying, expand numerous liquid spawn (this all is the conventional method of producing liquid spawn) of making through secondary then.
(3) inoculation is infected
Silkworm chrysalis was put into the disinfectant soaking disinfection made with ozone generator 5-10 minute, and the Cordyceps militaris liquid spawn with cultivating sucks liquid spawn with asepsis injector, injects the 0.05-0.3ml liquid spawn at the abdominal segment place of cassava silkworm chrysalis.
(4) stiff pupa is cultivated
The cassava silkworm chrysalis that infects is placed in the pallet, and one deck is put on many ground.In temperature is that 18-25 ℃ of relative air humidity is 60-70%, dark culturing.Little earlier stiff of pupa, stiff the moving back that pupa is big generally cultivated through 10-15 days, and the Cordyceps militaris mycelia all is full of pupal cell, and the cassava silkworm chrysalis is just stiff.
(5) going out grass cultivates
Stiff cassava silkworm chrysalis, put into the disinfectant soaking disinfection made with ozone generator 5-10 minute, put into the wide-mouth vial of 500ml then, one deck is put on many ground, seals with transparent membrane, and be that 18-25 ℃ of relative air humidity is 90-100% in temperature, intensity of illumination is to cultivate under the condition of 500-20001ux, cordycep militaris fruiting body entity primodium appearred in 10-15 days in process, continued to cultivate 25-30 days, just can gather when ascospore occurring etc. the fruit body head, dries, store.
Above example all can reach purpose of the present invention through repeatedly repeating, and can produce the cassava silkworm Cordyceps militaris.
Claims (2)
1. one kind is cassava silkworm Cordyceps militaris and the breeding method thereof that the host cultivates with the cassava silkworm chrysalis, it is characterized in that bacterial classification by Cordyceps militaris Coydyceps militaris (L.) Link, infect the cassava silkworm chrysalis and cultivate the cassava silkworm Cordyceps militaris that obtains, the mycelium that its cassava silkworm chrysalis yellowish-brown or sepia body surface are slightly yellow has clavate or multi-branched shape, orange-yellow fruit body in silkworm chrysalis body surface length.
2. one kind is the breeding method that the host cultivates the cassava silkworm Cordyceps militaris with the cassava silkworm chrysalis, and its feature may further comprise the steps:
(1), inject with asepsis injector in the cassava silkworm pupal cell of oneself sterilization of 0.05-0.3ml with the Cordyceps militaris liquid spawn that cultivates.
(2) the cassava silkworm chrysalis that infects being deployed in the pallet, is that 18-25 ℃, relative air humidity are under 60-70%, the dark condition in temperature, cultivates through 10-15 days, and the cassava silkworm chrysalis of infection is just stiff.
(3) select stiff cassava silkworm chrysalis, put into the disinfectant soaking disinfection made with ozone generator 5-10 minute, put into the wide-mouth vial of 500ml then, seal with transparent membrane, in temperature is that 18-25 ℃ of relative air humidity is 90-100%, and intensity of illumination is to cultivate under the condition of 500-2000lux, through occurring cordycep militaris fruiting body entity primodium in 10-15 days, continue to cultivate 25-30 days, the oven dry of just can gathering when ascospore occurring etc. the fruit body head is stored.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910113947A CN101849477A (en) | 2009-03-30 | 2009-03-30 | Cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as host and cultivating method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910113947A CN101849477A (en) | 2009-03-30 | 2009-03-30 | Cassava silkworm coydyceps militaris (L.) link cultivated by taking cassava silkworm chrysalis as host and cultivating method thereof |
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| CN101849477A true CN101849477A (en) | 2010-10-06 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430776A (en) * | 2013-09-04 | 2013-12-11 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
| CN103960055A (en) * | 2014-05-15 | 2014-08-06 | 高青和润丝绸有限公司 | Method for cultivating silkworm cordyceps |
| CN105359825A (en) * | 2013-05-15 | 2016-03-02 | 刘东波 | Silkworm insect grass cultivating method of infecting bombyxmori larva by cordyceps fungus strain |
| CN105706738A (en) * | 2016-02-23 | 2016-06-29 | 张家港顺泰元生物科技有限公司 | Cordycepsmilitaris cultivation method |
| WO2021025103A1 (en) * | 2019-08-07 | 2021-02-11 | 株式会社 沖縄Ｕｋａｍｉ養蚕 | Composition for inhibiting reduction of testosterone and/or dihydrotestosterone |
| CN114457172A (en) * | 2022-03-21 | 2022-05-10 | 江苏科技大学 | Castor silkworm EST-SSR molecular marker and application thereof |
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2009
- 2009-03-30 CN CN200910113947A patent/CN101849477A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359825A (en) * | 2013-05-15 | 2016-03-02 | 刘东波 | Silkworm insect grass cultivating method of infecting bombyxmori larva by cordyceps fungus strain |
| CN103430776A (en) * | 2013-09-04 | 2013-12-11 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
| CN103960055A (en) * | 2014-05-15 | 2014-08-06 | 高青和润丝绸有限公司 | Method for cultivating silkworm cordyceps |
| CN105706738A (en) * | 2016-02-23 | 2016-06-29 | 张家港顺泰元生物科技有限公司 | Cordycepsmilitaris cultivation method |
| WO2021025103A1 (en) * | 2019-08-07 | 2021-02-11 | 株式会社 沖縄Ｕｋａｍｉ養蚕 | Composition for inhibiting reduction of testosterone and/or dihydrotestosterone |
| CN114269364A (en) * | 2019-08-07 | 2022-04-01 | 日商冲绳羽化美养蚕股份有限公司 | Composition for inhibiting reduction of testosterone and/or dihydrotestosterone |
| CN114457172A (en) * | 2022-03-21 | 2022-05-10 | 江苏科技大学 | Castor silkworm EST-SSR molecular marker and application thereof |
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Application publication date: 20101006 |