CN108293606A - A kind of soil cultivation method of Cordyceps militaris potted landscape - Google Patents
A kind of soil cultivation method of Cordyceps militaris potted landscape Download PDFInfo
- Publication number
- CN108293606A CN108293606A CN201710957129.5A CN201710957129A CN108293606A CN 108293606 A CN108293606 A CN 108293606A CN 201710957129 A CN201710957129 A CN 201710957129A CN 108293606 A CN108293606 A CN 108293606A
- Authority
- CN
- China
- Prior art keywords
- cordyceps militaris
- soil
- strain
- potted landscape
- polypide
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention relates to a kind of soil cultivation methods of Cordyceps militaris potted landscape, specifically include：Strain is seeded in living silkworm chrysalises or other types of living worm body in gnotobasis, routinely cultural method culture to Chinese caterpillar fungus hypha is covered in polypide body；By illumination annesl, long former base, go out flower bud, long fructification and etc. after, the complex of cordyceps militaris sporocarp and polypide is transferred in beautiful potted landscape；The Nutrition Soil of disinfection is added, batten is inserted into or toothpick is fixed；After cultivating a period of time, after complex grows fructification, by being trimmed to pleasing soil cultivation Cordyceps militaris potted landscape.The Cordyceps militaris of this method cultivation serves not only as food materials, can also be fabricated to potted landscape and be used for watching, this method is at low cost, simple for process, and wherein cordycepin and SOD contents is higher.
Description
Technical field
The present invention relates to a kind of potted landscape technology of preparing of medicinal fungi and soil cultivation technical field, specially a kind of pupa worm
The soil cultivation method of careless potted landscape.
Background technology
Natural C.militaris is to infect live larva by cordyceps mycelia, draws Larval foods, is full of Cordyceps Militaris in larva body
Silk and it is dead, at stiff pupa（Worm）, under appropriate conditions, the mycelia of Cordyceps Militaris in stiff pupal cell growth and breeding at stroma, stroma after
Continuous to grow into rodlike fructification, outer likeness in form grass from head or other positions, complex is known as " Cordyceps militaris ".
Cordyceps militaris is the rarity of the Nature, is lived because it contains the biology such as abundant cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, SOD
Property substance, medical value highly significant.Cordycepin（3'-Deoxyadenosine）It is a kind of nucleosides material with antibacterial activity,
It has good effect in terms of protect liver, guarantor's kidney, moistening lung, while there is anticancer, pharmacology that is antiviral, removing free radical to make
With.Cordycepic acid（D-mannital）With cranium pressure is reduced, promote body metabolism, to make cerebral hemorrhage and cerebral thrombosis disease obtain
To alleviation.Cordyceps sinensis polysaccharide（Galactomannan polysaccharide）Can activated macrophage stimulation blood in antibody generate, improve human body exempt from
Epidemic disease ability improves respiratory system.SOD（Superoxide dismutase）Superoxide radical in body can be eliminated, there is anti-aging, resist
Cancer presses down the effect of cancer.In addition, its antitumaous effect of the distinctive cordycepin of Cordyceps militaris is universally acknowledged, by NCI（American National cancer
Disease research institute）And Boston Univ USA is used for the clinical trial of anti-leukocythemia, cancer.
Since the medicinal and health value of Cordyceps militaris obtains approval both domestic and external, and the Cordyceps militaris grown naturally cannot meet
The needs in market, therefore the mode of cultivation can only be explored.By the effort of researcher for many years, in cultivation Cordyceps militaris
Technical acquisition important breakthrough, and the pharmaceutical ingredient content of Cordyceps militaris can be greatly improved.
Currently, the pattern of cultivation is mainly carried out as culture medium without soil using rice, wheat, corn and soybean etc.
Cultivation, but its with high costs, easy to pollute and active material（Cordycepin, SOD etc.）Content is relatively low, once pollution in wide area, enterprise
Huge loss will be faced by cultivating family with individual, and activity substance content is low, and medical value is very low.
The purposes of Cordyceps militaris modes such as edible primarily as Chinese medicinal material or directly, however cordyceps militaris sporocarp is bright-colored
It is beautiful, external form is varied, there is erect type, flexure type, branch type, chiasma type, coral type etc., also have megaspore head, conidia powder,
The kind of multiple and different appearances such as sharp grass, it is therefore, not only edible, exquisite artistical bonsai can also be fabricated to.
Now report discloses the Soilless Culture Methods of a variety of Cordyceps militaris, and the mode for carrying out soil cultivation there is no report.
Invention content
The purpose of the present invention is to provide a kind of soil cultivation methods of completely new Cordyceps militaris potted landscape, solve current pupa worm
The single deficiency brought without soilization planting type of grass, serves not only as food materials, can also be fabricated to potted landscape and be used for watching.It should
Method is at low cost, simple for process, cannot be only used for watching, and wherein cordycepin and SOD contents is higher.
The present invention is mainly achieved through the following technical solutions：
Cordyceps species used in the present invention, come from the strain library of Changde Yandi Biotechnology Co., Ltd., and specific strain is
One kind in megaspore head kind, microspore head kind, conidia powder kind, sharp grass product kind, thin grass product kind, longleaf luckyweed flower kind.This
Method is as follows：
(1) nutrient solution is configured：By glucose 2%, peptone 0.5%, beef extract 0.5%, epsom salt 0.1%, potassium dihydrogen phosphate
0.1%, vitamin b10.001%, vitamin b120.001%, water 96.798% forms, and is uniformly mixed；
(2) nutrient solution is dispensed into triangular flask, autoclave sterilization 20~30 minutes, taking-up is cooled to room temperature spare；
(3) it takes out strain strain is seeded in above-mentioned nutrient solution in gnotobasis, seal；
(4) strain is placed on shaking table, 120~150rpm of rotating speed is set, and 17~25 DEG C of temperature, shaking flask culture 96~120 is small
When；
(5) after cleaning up living worm body, it is sterilized using 75% alcohol in gnotobasis, the culture of packing to sterilizing
In bottle；
(6) strain that step (4) obtains is seeded to by inoculator in living worm body body, is prepared into Cordyceps militaris complex, it is close
Envelope；
(7) in the environment of the Cordyceps militaris complex prepared being transferred to 17~25 DEG C of temperature, humidity 65~85%, light culture 5~
7 days, polypide surface grew a large amount of white hyphas, you can；
(8) under conditions of step (7), 200~2000lux of intensity of illumination is set, light-exposed annesl is carried out, generated by former base,
Go out flower bud growth, go out grass growth three phases, cordyceps militaris sporocarp is grown to 1~1.5cm height；
(9) sandy soil are taken, by sand：Soil=1:After 4 ratio mixing, it is placed in autoclave, 100 DEG C, 8 hours or 121 DEG C of setting,
Sterilization treatment is carried out within 30 minutes, it is spare after cooling；
(10) Cordyceps militaris complex is transferred in Potted landscape vessel, sandy soil of the covering by step (9) processing around polypide；
(11) polypide is fixed in soil by the batten or toothpick that disinfection is inserted through on polypide, trims extra batten or tooth
Label,
By sandy soil：Water=20：1 ratio sprays clear water against sandy soil, while slightly spraying fructification using clear water, and it is real to remove son
Body
On the soil be stained with；
(12) it waits for that cordyceps militaris sporocarp is grown to 4~5cm height, then fructification is trimmed to various artistic shapes.
The step（5）In living worm body be tussah chrysalis, it is mulberry silkworm chrysalis, one or more in meat maggot.
The present invention has the advantages that following 7 is big：
(1) present invention changes the pattern that Cordyceps militaris is cultivated in single soilless culture, uses a kind of mould of completely new soil cultivation
Formula is cultivated；
(2) soil cultivation Cordyceps militaris of the invention is of low cost, is worth very high after cultivating successfully, and the economy of several times may be implemented
Benefit；
(3) present invention utilizes the characteristic of sterile silt, it is possible to reduce culture medium pollutes, and reduces risk cost；
(4) the potted landscape preparation method that the present invention is made using Cordyceps militaris is simple, easy to operate；After potted landscape prepares, only need to water
, general indoor spaces can cultivate；
(5) it by applying the present invention, changing edible way single in the market, acts not only as in art work display room,
It is also used as that condiment stews, cooks gruel, quick-fried is eaten after its maturation, can also be brewed after drying and work as tea, health value
It can also be embodied；
(6) after the fruiting bodies of cordyceps militaris maturation that the present invention nurtures, cordycepin and SOD contents are higher, and medical value is larger；
(7) it has no the disclosed report of the cordyceps sinensis potted landscape of soil cultivation, there is prodigious market potential, be worthy to be popularized.
Specific implementation mode
In order to make the objectives, technical solutions and advantages of the present invention clearer, With reference to embodiment, to this
Invention is further described.It should be understood that these descriptions are merely illustrative, and it is not intended to limit the scope of the present invention.
Embodiment 1：
(1) nutrient solution is configured：By glucose 2%, peptone 0.5%, beef extract 0.5%, epsom salt 0.1%, potassium dihydrogen phosphate
0.1%, vitamin b10.001%, vitamin b120.001%, water 96.798% forms, and is uniformly mixed；
(2) nutrient solution is dispensed into triangular flask, autoclave sterilization 20 minutes, taking-up is cooled to room temperature spare；
(3) cordyceps species are taken out（The cordyceps species come from the megaspore in Changde Yandi Biotechnology Co., Ltd.'s strain library
Head kind）, in gnotobasis, strain is seeded in above-mentioned nutrient solution, is sealed；
(4) strain is placed on shaking table, rotating speed 120rpm, 17 DEG C of temperature, shaking flask culture 96 hours is set；
(5) after cleaning up living worm body, it is sterilized using 75% alcohol in gnotobasis, the culture of packing to sterilizing
In bottle；
(6) strain that step (4) obtains is seeded to by inoculator in living worm body body, is prepared into Cordyceps militaris complex, it is close
Envelope；
(7) in the environment of the Cordyceps militaris complex prepared being transferred to 17 DEG C of temperature, humidity 65%, light culture 5 days, polypide table
Face grows a large amount of white hyphas, you can；
(8) under conditions of step (7), intensity of illumination 200lux is set, light-exposed annesl is carried out, generated by former base, go out flower bud life
Length goes out grass growth three phases, and cordyceps militaris sporocarp is grown to 1~1.5cm height；
(9) sandy soil are taken, by sand：Soil=1:It after 4 ratio mixing, is placed in autoclave, setting sterilizes for 100 DEG C, 8 hours
Processing, it is spare after cooling；
(10) Cordyceps militaris complex is transferred in Potted landscape vessel, sandy soil of the covering by step (9) processing around polypide；
(11) polypide is fixed in soil by the batten or toothpick that disinfection is inserted through on polypide, trims extra batten or tooth
Label,
By sandy soil：Water=20：1 ratio sprays clear water against sandy soil, while slightly spraying fructification using clear water, and it is real to remove son
Body
On the soil be stained with；
(12) it waits for that cordyceps militaris sporocarp is grown to 4~5cm height, then fructification is trimmed to various artistic shapes.
Embodiment 2：
(1) nutrient solution is configured：By glucose 2%, peptone 0.5%, beef extract 0.5%, epsom salt 0.1%, potassium dihydrogen phosphate
0.1%, vitamin b10.001%, vitamin b120.001%, water 96.798% forms, and is uniformly mixed；
(2) nutrient solution is dispensed into triangular flask, autoclave sterilization 30 minutes, taking-up is cooled to room temperature spare；
(3) cordyceps species are taken out（The cordyceps species come from the microspore in Changde Yandi Biotechnology Co., Ltd.'s strain library
Head kind）, in gnotobasis, strain is seeded in above-mentioned nutrient solution, is sealed；
(4) strain is placed on shaking table, rotating speed 150rpm, 25 DEG C of temperature, shaking flask culture 120 hours is set；
(5) after cleaning up living worm body, it is sterilized using 75% alcohol in gnotobasis, the culture of packing to sterilizing
In bottle；
(6) strain that step (4) obtains is seeded to by inoculator in living worm body body, is prepared into Cordyceps militaris complex, it is close
Envelope；
(7) in the environment of the Cordyceps militaris complex prepared being transferred to 25 DEG C of temperature, humidity 85%, light culture 7 days, polypide table
Face grows a large amount of white hyphas, you can；
(8) under conditions of step (7), intensity of illumination 2000lux is set, light-exposed annesl is carried out, generated by former base, go out flower bud life
Length goes out grass growth three phases, and cordyceps militaris sporocarp is grown to 1~1.5cm height；
(9) sandy soil are taken, by sand：Soil=1:It after 4 ratio mixing, is placed in autoclave, setting is gone out for 121 DEG C, 30 minutes
Bacterium is handled, spare after cooling；
(10) Cordyceps militaris complex is transferred in Potted landscape vessel, sandy soil of the covering by step (9) processing around polypide；
(11) polypide is fixed in soil by the batten or toothpick that disinfection is inserted through on polypide, trims extra batten or tooth
Label,
By sandy soil：Water=20：1 ratio sprays clear water against sandy soil, while slightly spraying fructification using clear water, and it is real to remove son
Body
On the soil be stained with；
(12) it waits for that cordyceps militaris sporocarp is grown to 4~5cm height, then fructification is trimmed to various artistic shapes.
Embodiment 3：
(1) nutrient solution is configured：By glucose 2%, peptone 0.5%, beef extract 0.5%, epsom salt 0.1%, potassium dihydrogen phosphate
0.1%, vitamin b10.001%, vitamin b120.001%, water 96.798% forms, and is uniformly mixed；
(2) nutrient solution is dispensed into triangular flask, autoclave sterilization 25 minutes, taking-up is cooled to room temperature spare；
(3) cordyceps species are taken out（The cordyceps species come from the longleaf luckyweed flower in Changde Yandi Biotechnology Co., Ltd.'s strain library
Kind）, in gnotobasis, strain is seeded in above-mentioned nutrient solution, is sealed；
(4) strain is placed on shaking table, rotating speed 130rpm, 20 DEG C of temperature, shaking flask culture 110 hours is set；
(5) after cleaning up living worm body, it is sterilized using 75% alcohol in gnotobasis, the culture of packing to sterilizing
In bottle；
(6) strain that step (4) obtains is seeded to by inoculator in living worm body body, is prepared into Cordyceps militaris complex, it is close
Envelope；
(7) in the environment of the Cordyceps militaris complex prepared being transferred to 20 DEG C of temperature, humidity 75%, light culture 6 days, polypide table
Face grows a large amount of white hyphas, you can；
(8) under conditions of step (7), intensity of illumination 1000lux is set, light-exposed annesl is carried out, generated by former base, go out flower bud life
Length goes out grass growth three phases, and cordyceps militaris sporocarp is grown to 1~1.5cm height；
(9) sandy soil are taken, by sand：Soil=1:It after 4 ratio mixing, is placed in autoclave, setting sterilizes for 100 DEG C, 8 hours
Processing, it is spare after cooling；
(10) Cordyceps militaris complex is transferred in Potted landscape vessel, sandy soil of the covering by step (9) processing around polypide；
(11) polypide is fixed in soil by the batten or toothpick that disinfection is inserted through on polypide, trims extra batten or tooth
Label,
By sandy soil：Water=20：1 ratio sprays clear water against sandy soil, while slightly spraying fructification using clear water, and it is real to remove son
Body
On the soil be stained with；
(12) it waits for that cordyceps militaris sporocarp is grown to 4~5cm height, then fructification is trimmed to various artistic shapes.
The following table 1 is the correction data of 3 embodiments of this patent and 3 groups of control groups of conventionally employed wheat broth cultivation
Table 1：
According to 1 result of upper table it is found that fruiting bodies of cordyceps militaris its yield for nurturing of the present invention is higher, pollution rate is relatively low, therefore,
Its is of low cost；Its SOD vigor, cordycepin content are higher simultaneously, and medical value is also very high.
Although embodiments of the present invention are described in detail, it should be understood that, without departing from the present invention's
In the case of spirit and scope, can embodiments of the present invention be made with various changes, replacement and change.
Claims (2)
1. a kind of soil cultivation method of Cordyceps militaris potted landscape, which is characterized in that include the following steps：
(1) nutrient solution is configured：By glucose 2%, peptone 0.5%, beef extract 0.5%, epsom salt 0.1%, potassium dihydrogen phosphate
0.1%, vitamin b10.001%, vitamin b120.001%, water 96.798% forms, and is uniformly mixed；
(2) nutrient solution is dispensed into triangular flask, autoclave sterilization 20~30 minutes, taking-up is cooled to room temperature spare；
(3) it takes out strain strain is seeded in above-mentioned nutrient solution in gnotobasis, seal；Cordyceps sinensis used in the present invention
Strain, comes from the strain library of Changde Yandi Biotechnology Co., Ltd., and specific strain is megaspore head kind, microspore head product
One kind in kind, conidia powder kind, sharp grass product kind, thin grass product kind, longleaf luckyweed flower kind；
(4) strain is placed on shaking table, 120~150rpm of rotating speed is set, and 17~25 DEG C of temperature, shaking flask culture 96~120 is small
When；
(5) after cleaning up living worm body, it is sterilized using 75% alcohol in gnotobasis, the culture of packing to sterilizing
In bottle；
(6) strain that step (4) obtains is seeded to by inoculator in living worm body body, is prepared into Cordyceps militaris complex, it is close
Envelope；
(7) in the environment of the Cordyceps militaris complex prepared being transferred to 17~25 DEG C of temperature, humidity 65~85%, light culture 5~
7 days, polypide surface grew a large amount of white hyphas, you can；
(8) under conditions of step (7), 200~2000lux of intensity of illumination is set, light-exposed annesl is carried out, generated by former base,
Go out flower bud growth, go out grass growth three phases, cordyceps militaris sporocarp is grown to 1~1.5cm height；
(9) sandy soil are taken, by sand：Soil=1:After 4 ratio mixing, it is placed in autoclave, 100 DEG C, 8 hours or 121 DEG C of setting,
Sterilization treatment is carried out within 30 minutes, it is spare after cooling；
(10) Cordyceps militaris complex is transferred in Potted landscape vessel, sandy soil of the covering by step (9) processing around polypide；
(11) polypide is fixed in soil by the batten or toothpick that disinfection is inserted through on polypide, trims extra batten or tooth
Label,
By sandy soil：Water=20：1 ratio sprays clear water against sandy soil, while slightly spraying fructification using clear water, and it is real to remove son
Body
On the soil be stained with；
(12) it waits for that cordyceps militaris sporocarp is grown to 4~5cm height, then fructification is trimmed to various artistic shapes.
2. a kind of soil cultivation method of Cordyceps militaris potted landscape according to claim 1, which is characterized in that the step（5）
In living worm body be tussah chrysalis, it is mulberry silkworm chrysalis, one or more in meat maggot.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN115005012A (en) * | 2022-07-21 | 2022-09-06 | 重庆华绿北草生物科技有限公司 | High cordycepin cordyceps militaris culture method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN115005012A (en) * | 2022-07-21 | 2022-09-06 | 重庆华绿北草生物科技有限公司 | High cordycepin cordyceps militaris culture method |
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Application publication date: 20180720 |