CN110226459A - A kind of cultural method of cicada fungus conidia powder - Google Patents
A kind of cultural method of cicada fungus conidia powder Download PDFInfo
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- CN110226459A CN110226459A CN201910598899.4A CN201910598899A CN110226459A CN 110226459 A CN110226459 A CN 110226459A CN 201910598899 A CN201910598899 A CN 201910598899A CN 110226459 A CN110226459 A CN 110226459A
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- cicada fungus
- powder
- conidia powder
- spore
- cicada
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of cultural methods of cicada fungus conidia powder, are primary raw material using rice flour, corn flour, and addition bean powder, cicada fungus powder are culture substrate, and adjusting water content is 30%~40%, and pH value is 5.0~6.0；Culture substrate is mixed and is bottled, it is cooling after high pressure sterilization, cicada fungus spore strain is accessed, after culture in 50 days is mature, harvests cicada fungus conidia powder.This method is easy to operate, with short production cycle, at low cost, and cicada fungus conidia powder is clean and with high purity, has high application value.
Description
Technical field
The invention belongs to mushroom cultivation fields, and in particular to a kind of cultural method of cicada fungus conidia powder.
Background technique
Cicada fungus conidia powder is cicada fungus " seed ", has breeding function, cicada fungus conidia powder is by the activity of sclerotium, coremium
Ingredient gradates, and has accumulated the active essence of cicada fungus, is cicada fungus active pharmaceutical ingredient, the highest part of effect.Have
Antitumor action.Cicada fungus conidia powder was gradually available for the clinical research of tumor area by medical worker in recent years, in state in 2011
In border biological medicine and the annual meeting of biotechnology forum, pharmaceutical college, University Of Suzhou professor Gu Zhenlun reports its scientific achievement " golden cicada
Flower conidia powder and wild golden cicada flower Thick many candies antitumor action preliminary report " in point out, cicada fungus conidia powder is to cancer of pancreas, white blood
The apoptosis rate (orderly cancer cell death) of disease and cervical cancer cell is 8.24 times of broad-spectrum anti-cancer drug 5-Fu, 16.64 times respectively
With 23.44 times;Necrosis rate (killing) to cancer cell is 8.67 times, 287.75 times and 31.50 times of 5-Fu respectively.Cicada fungus at present
Conidia powder culture technique has not been reported, and research cicada fungus conidia powder cultural method has important value.
Cicada fungus has raising immunity, antifatigue, guarantor's kidney, improvement, sleep, antitumor, liver protection, anti-radiation and improving eyesight etc. more
Recast is used, and is magical ancient Chinese medicine.The cicada fungus manually cultivated contain mannitol 2.18%, polysaccharide 21.73%, amino acid 1 9.76%,
Cordycepin 0.005%, adenosine 0.05%, this is similar to natural cordyceps, and therefore, cicada fungus can be used as the alternative of cordyceps sinensis
Product equally can achieve the effect of nourishing and health preserving.
Summary of the invention
The purpose of the present invention is to provide a kind of cultural methods of cicada fungus conidia powder, and this method is easy to operate, the production cycle
Short, at low cost, cicada fungus conidia powder is clean and with high purity, has high application value.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of cultural method of cicada fungus conidia powder the following steps are included:
1) culture of CSM treated body: using rice flour, corn flour as primary raw material, addition soy meal, cicada fungus powder are culture substrate, are adjusted
It is 5.0~6.0 that section water content, which is 30%~40%, pH value,；Culture substrate is mixed and is bottled, expects high 1~1.5cm, high pressure sterilization
After cool down, access cicada fungus spore strain, at 25 DEG C darkroom cultivate 10~15d, until culture substrate covers with CSM treated body；
2) culture of cicada fungus conidia powder and harvesting: control environmental condition, create suitable temperature, humidity, illumination, ventilation culture item
Part harvests cicada fungus conidia powder after CSM treated body maturation.
Each raw material percentage in step 1), contains rice flour 40%~50%, corn flour 20%~30%, soy meal
3%~7%, cicada fungus powder 0.5%~1.5%, sterile water 24%.
Cicada fungus spore strain in step 1) adopts the wild cicada fungus of the longevity mountain village Yu Fuzhou bamboo grove, and through spore separation, purifying is obtained
Spore strain, the according to a conventional method production of hybrid seeds, culture.
The temperature for being suitable in step 2 is 18 DEG C~23 DEG C；5 DEG C/d of the temperature difference；Air humidity 65%；Intensity of illumination be 50~
80lux；Fructification occur after controlled at 20 DEG C, air humidity 70%；Intensity of illumination is 100lux；Daily mushroom house of ventilating is logical
Gas is primary, and half an hour time was to one hour.
Cicada fungus conidia powder is harvested in step 2.After stromal surface has coremium and white powder spore generates, open
Bottle cap, 60 DEG C be dried to 7-8 at, scan out coremium and conidia powder with hairbrush, by 100 mesh sieve be sieved, obtain pure cicada fungus spore
Sub- powder.
Remarkable advantage of the invention is: method is easy to operate, with short production cycle, at low cost, cicada fungus conidia powder it is clean and
Purity is high has high application value.
Specific embodiment
Embodiment 1
A kind of cicada fungus conidia powder cultural method the following steps are included:
1) culture of CSM treated body body: using rice flour, corn flour as primary raw material, addition bean powder, cicada fungus powder are culture substrate, are adjusted
It is 5.5 that section water content, which is 35pH value,；Culture substrate is mixed and is bottled, expects high 1cm, cooling, access cicada fungus cicada after high pressure sterilization
Flower strain, 10~15d is cultivated in darkroom at 25 DEG C, until culture substrate covers with CSM treated body；
2) culture of cicada fungus conidia powder and harvesting: control environmental condition, create suitable temperature, humidity, illumination, ventilation culture item
Part harvests cicada fungus conidia powder after CSM treated body maturation.
Each raw material percentage in step 1), contains rice flour 45%, corn flour 25%, soy meal 5%, cicada fungus powder
1%, sterile water 24%；
Cicada fungus spore strain in step 1) adopts the wild cicada fungus of the longevity mountain village Yu Fuzhou bamboo grove, and through spore separation, purifying obtains spore
Strain, the according to a conventional method production of hybrid seeds, culture.
The temperature for being suitable in step 2 is 18 DEG C~23 DEG C；5 DEG C/d of the temperature difference；Air humidity 65%；Intensity of illumination is
70lux；Fructification occur after controlled at 20 DEG C, air humidity 70%；Intensity of illumination is 100lux；Daily mushroom house of ventilating is logical
Gas is primary, and half an hour time was to one hour.
Cicada fungus conidia powder is harvested in step 2.After stromal surface has coremium and white powder spore generates, open
Bottle cap, 60 DEG C are dried to 8 one-tenth, scan out coremium and conidia powder with hairbrush, are sieved by 100 mesh sieve, obtain pure cicada fungus spore
Powder.
Embodiment 2
A kind of cicada fungus conidia powder cultural method the following steps are included:
1) culture of CSM treated body body: using rice flour, corn flour as primary raw material, addition bean powder, cicada fungus powder are culture substrate, are adjusted
It is 5.5 that section water content, which is 35%, pH value,；Culture substrate is mixed and is bottled, expects high 1cm, cooling, access cicada fungus after high pressure sterilization
Spore strain, 10~15d is cultivated in darkroom at 25 DEG C, until culture substrate covers with CSM treated body；
2) culture of cicada fungus conidia powder and harvesting: control environmental condition, create suitable temperature, humidity, illumination, ventilation culture item
Part harvests cicada fungus conidia powder after CSM treated body maturation.
Each raw material percentage in step 1), contains rice flour 50%, corn flour 20%, soy meal 4.5%, cicada fungus powder
1.5%, sterile water 24%.
Cicada fungus spore strain in step 1) adopts the wild cicada fungus of the longevity mountain village Yu Fuzhou bamboo grove, and through spore separation, purifying is obtained
Spore strain, the according to a conventional method production of hybrid seeds, culture.
The temperature for being suitable in step 2 is 18 DEG C~23 DEG C；5 DEG C/d of the temperature difference；Air humidity 65%；Intensity of illumination is
70lux；Fructification occur after controlled at 20 DEG C, air humidity 70%；Intensity of illumination is 100lux；Daily mushroom house of ventilating is logical
Gas is primary, and half an hour time was to one hour.
Cicada fungus conidia powder is harvested in step 2.After stromal surface has coremium and white powder spore generates, open
Bottle cap, 60 DEG C are dried to 8 one-tenth, scan out coremium and conidia powder with hairbrush, are sieved by 100 mesh sieve, obtain pure cicada fungus spore
Powder.
Embodiment 3
A kind of cicada fungus conidia powder cultural method the following steps are included:
1) culture of CSM treated body body: using rice flour, corn flour as primary raw material, addition bean powder, cicada fungus powder are culture substrate, are adjusted
It is 5.5 that section water content, which is 35%, pH value,；Culture substrate is mixed and is bottled, expects high 1cm, cooling, access cicada fungus after high pressure sterilization
Strain, 10~15d is cultivated in darkroom at 25 DEG C, until culture substrate covers with CSM treated body；
2) culture of cicada fungus conidia powder and harvesting: control environmental condition, create suitable temperature, humidity, illumination, ventilation culture item
Part harvests cicada fungus conidia powder after CSM treated body maturation.
Each raw material percentage in step 1), contains rice flour 40%, corn flour 30%, soy meal 5.5%, cicada fungus powder
0.5%, sterile water 24%.
Environment Conditions Temperature is 18 DEG C~23 DEG C in step 2,5 DEG C/d of the temperature difference；Intensity of illumination is 70x；Fructification occurs
Afterwards controlled at 20 DEG C, intensity of illumination 100lux；Daily mushroom house ventilation is primary, and half an hour time was to one hour.Work as stroma
Surface harvests cicada fungus conidia powder after having the generation of white powder spore.
The temperature for being suitable in step 2 is 18 DEG C~23 DEG C；5 DEG C/d of the temperature difference；Air humidity 65%；Intensity of illumination is
70lux；Fructification occur after controlled at 20 DEG C, air humidity 70%；Intensity of illumination is 100lux；Daily mushroom house of ventilating is logical
Gas is primary, and half an hour time was to one hour.
Cicada fungus conidia powder is harvested in step 2.After stromal surface has coremium and white powder spore generates, open
Bottle cap, 60 DEG C are dried to 8 one-tenth, scan out coremium and conidia powder with hairbrush, are sieved by 100 mesh sieve, obtain pure cicada fungus spore
Powder.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (5)
1. a kind of cultural method of cicada fungus conidia powder, it is characterised in that: the following steps are included:
1) culture of CSM treated body: using rice flour, corn flour as primary raw material, addition soy meal, cicada fungus powder are culture substrate, are adjusted
It is 5.0~6.0 that section water content, which is 30%~40%, pH value,；Culture substrate is mixed and is bottled, expects high 1~1.5cm, high pressure sterilization
After cool down, access cicada fungus spore strain, at 25 DEG C darkroom cultivate 10~15d, until culture substrate covers with CSM treated body；
2) culture of cicada fungus conidia powder and harvesting: control temperature, humidity, illumination, duration of ventilation are adopted after CSM treated body maturation
Receive cicada fungus conidia powder.
2. the cultural method of the cicada fungus conidia powder according to claim 1, it is characterised in that: each raw material is pressed in step 1)
Percent by weight: rice flour 40%~50%, corn flour 20%~30%, soy meal 3%~7%, cicada fungus powder 0.5%~1.5%, sterile water
24%。
3. the cultural method of the cicada fungus conidia powder according to claim 1, it is characterised in that: cicada fungus spore in step 1)
Strain adopts the wild cicada fungus of the longevity mountain village Yu Fuzhou bamboo grove, and through spore separation, purifying obtains spore strain, according to a conventional method the production of hybrid seeds, training
It supports.
4. the cultural method of the cicada fungus conidia powder according to claim 1, it is characterised in that: control temperature in step 2
It is 18 DEG C~23 DEG C；5 DEG C/d of the temperature difference；Air humidity 65%；Intensity of illumination is 50~80lux；Fructification occur after controlled at
20 DEG C, air humidity 70%；Intensity of illumination is 100lux；Ventilation is primary daily, 0.5~1h of time.
5. the cultural method of the cicada fungus conidia powder according to claim 1, it is characterised in that: when matrix table in step 2
After face has coremium and white powder spore to generate, bottle cap is opened, 60 DEG C are dried to 7-8 into dry, taking-up coremium and spore
Powder sieves with 100 mesh sieve, and obtains pure cicada fungus conidia powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112715276A (en) * | 2021-01-08 | 2021-04-30 | 浙江泛亚生物医药股份有限公司 | Culture method of cordyceps sobolifera spore powder |
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