CN103598014A - Production method of artificial isaria cicadae miq - Google Patents
Production method of artificial isaria cicadae miq Download PDFInfo
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- CN103598014A CN103598014A CN201310597913.1A CN201310597913A CN103598014A CN 103598014 A CN103598014 A CN 103598014A CN 201310597913 A CN201310597913 A CN 201310597913A CN 103598014 A CN103598014 A CN 103598014A
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Abstract
The invention discloses a production method of artificial isaria cicadae miq. The production method has the advantages that the technical parameters, cultivation medium conditions and the like of the method are obtained after years of optimization, each parameter, cultivation medium proportion and production conditions are quite scientific and precise, and tests show that high-quality artificial isaria cicadae miq can be obtained by the method; the method is short in cultivation time, the cultivation medium is rice, wheat or mixture of wheat and yeast, the auxiliary materials are easy to get and low in cost, and massive industrial production can be performed; the artificial isaria cicadae miq produced by the method can be used as food, healthcare product, and medicine, or as the auxiliary materials of foods, healthcare products and medicines.
Description
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Technical field
The present invention relates to the production method of a kind of artificial golden cicada fungus, belong to the artificial production technical field of medicine-food two-purpose fungi.
Background technology
Cicada fungus (CordycepsciecadaeShing) also claims cicada bacterium cicada pupa grass, it is the larva body that a kind of marvellous biological root with animal and plant feature is cicada pupa or mountain cicada, flower is from single or 2-3 cicada larva head grows out, and about one-inch how long, the branch of blooming from top.According to relevant researching and proposing, cicada fungus belongs to biological ill phenomenon, is the interdependent assembly of a kind of entomogenous fungi.Arrive autumn, cicada pierces in soil, becomes gradually cicada pupa, parasitic by Cordyceps sinensis mushroom before emergence, when life spare part is suitable, just starts to sprout into mycelium, absorbs the nutrition of polypide, and final polypide is occupied completely by mycelium and only remains the next body.In all things on earth recovery time, mycelium is converted into the perfect stage from the vegetative phase again, and the branch of gradually blooming from top, so the cicada fungus of gaining the name.
The wild golden cicada fungus of existing market, have three features distincter: the one, because it is a kind of of Chinese caterpillar fungus, there is certain analogy with traditional Cordyceps sinensis, price is carried out according to the price rule of Cordyceps sinensis substantially, finally likely can become second Cordyceps sinensis, most of consumer cannot bear the costliness of price; The 2nd, along with the intensification of exploitation, wild golden cicada fungus resource can reduce in a large number, will greatly limit the scope of application; The 3rd, along with the contract system in national mountain region, the environmental pollutions such as the production of bamboo product and soil, cause the probability of the pollution of wild golden cicada fungus residue of pesticide and heavy metal greatly to increase.For meeting the demand in market, be mankind's health service, manually cultivating golden cicada fungus will have important value and realistic meaning.
Summary of the invention
Object of the present invention is just to provide the production method of a kind of artificial golden cicada fungus, has production cost artificial cultivation gold cicada fungus culture technique low, with short production cycle, and this production method has been assigned to by following steps:
1) by wild golden cicada fungus low temperature dehumidification, temperature is below 25 ℃, and humidity, below 45%, is dried, and wild golden cicada fungus selects that conidial powder is more, color is whiter;
2) dried wild golden cicada fungus conidial powder is collected;
3) conidial powder after collection is in zero degree Refrigerator store, and the used time takes;
4) medium treatment: according to wheat or the rice of peeling off: dusty yeast be take the ratio that weight ratio is 5-10:1 and mixed, then carries out high-temperature sterilization processing, standby;
5) inoculation: according to conidial powder: medium be take weight ratio as 1:10000 ratio mixes, and mixed medium is positioned over and is cultivated in room, controls temperature at 15-25 ℃, and controlled humidity is at 65-85%, and incubation time is 20-30 days;
6) collection of culture is artificial golden cicada fungus and obtains thing, contains: coremium, conidial powder, medium and mycelia mixture.
In step 4) medium treatment, with 121 ℃ of high-temperature sterilizations, process.
Resulting golden cicada fungus can be used as food, health products, medicine use, or uses as the auxiliary material of food, health products, medicine.
Artificial golden cicada fungus production method technical parameter of the present invention and culture medium condition etc. obtain through optimizing for many years, parameters and Medium Proportion and working condition be scientific and precise very, through verification experimental verification, production can realize and can obtain high-quality artificial culture.Cultivated days of the present invention is shorter, and medium is rice common on market, wheat or is the mixture of wheat, dusty yeast, auxiliary material be easy to get and Costco Wholesale lower, can carry out in a large number suitability for industrialized production.
Embodiment
embodiment 1
1) collect or wild golden cicada fungus that buying is arrived, select conidial powder more, color is whiter, by the method for low temperature dehumidification (20 ℃ below, humidity below 45%), be dried;
2) dried wild golden cicada fungus conidial powder is collected;
3) conidial powder after collection is in zero degree Refrigerator store (long preservation cryogenic freezing), and the used time takes out;
4) medium treatment: according to the wheat of peeling off: dusty yeast 5:1 ratio altogether 50kg is mixed, then carry out 121 ℃ of high-temperature sterilizations (boiling) and process, standby;
5) inoculation: get conidial powder 5.0g, medium 50kg, mixed medium is positioned over to be cultivated in room, controls temperature at 15-25 ℃, and controlled humidity is at 65-75%, and incubation time is 22 days;
6) collection of culture is artificial golden cicada fungus, contains: coremium, conidial powder, medium and mycelia mixture;
7) resulting culture is dried under low temperature dehumidification condition (below 25 ℃, humidity below 45%), and be 24---48 hour drying time;
8) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
embodiment 2
1) collect or wild golden cicada fungus that buying is arrived, select conidial powder more, white color, by the method for low temperature dehumidification (25 ℃ below, humidity below 45%), be dried;
2) dried wild golden cicada fungus conidial powder is collected;
3) conidial powder after collection is in zero degree Refrigerator store (cryogenic freezing during long preservation), and the used time takes out;
4) medium treatment: according to the wheat of peeling off: dusty yeast 10:1 ratio altogether 50kg is mixed, then carry out 121 ℃ of high-temperature sterilizations (boiling) and process
5) inoculation: get conidial powder 5g, medium 50kg, is positioned over mixed medium to cultivate in room, controls temperature at 15-25 ℃, and controlled humidity is at 75-85%, and incubation time is 20---30 days;
6) collection of culture is artificial golden cicada fungus, contains: coremium, conidial powder, medium and mycelia mixture; ;
7) resulting culture is dried under low temperature dehumidification condition (below 25 ℃, humidity below 45%), and be 24--48 hour drying time;
8) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
embodiment 3
1) collect or wild golden cicada fungus that buying is arrived, select conidial powder more, color is whiter, by the method for low temperature dehumidification (25 ℃ below, humidity below 45%), be dried;
2) by dried wild collection for golden cicada fungus conidial powder;
3) conidial powder after collection is in zero degree Refrigerator store, and (the cryogenic freezing during long preservation) used time takes out;
4) medium treatment: the wheat 50kg that peels off carries out 121 ℃ of high-temperature sterilizations (boiling) to be processed;
5) inoculation: get conidial powder 5g, medium 50kg, is positioned over mixed medium to cultivate in room, controls temperature at 19-20 ℃, and controlled humidity is at 75-85%, and incubation time is 25 days;
6) collection of culture is artificial golden cicada fungus;
7) resulting culture is dried under low temperature dehumidification condition (below 20 ℃, humidity below 45%), and be 36 hours drying time;
8) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
embodiment 4
1) the artificial golden cicada fungus conidial powder of collecting, is dried by the method for low temperature dehumidification (25 ℃ following, humidity 45% below), seals 0 ℃ of preservation, the taking-up of (cryogenic freezing during long preservation) used time;
2) medium treatment: according to the wheat of peeling off: dusty yeast 5:1 ratio altogether 50kg is mixed, then carry out 121 ℃ of high-temperature sterilizations (boiling) process, standby;
3) inoculation: get conidial powder 5g, medium 50kg, is positioned over mixed medium to cultivate in room, controls temperature at 15-25 ℃, and controlled humidity is at 65-75%, and incubation time is 22 days;
4) collection of culture is artificial golden cicada fungus and obtains thing;
5) resulting culture is dried under low temperature dehumidification condition (below 25 ℃, humidity below 45%), and be 36 hours drying time;
6) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
embodiment 5
1) the artificial golden cicada fungus conidial powder of collecting, is dried by the method for low temperature dehumidification (25 ℃ following, humidity 45% below), seals 0 ℃ of preservation, used time taking-up;
2) medium treatment: according to the wheat of peeling off: dusty yeast 10:1 ratio altogether 50kg is mixed, then carry out 121 ℃ of high-temperature sterilizations (boiling) process, standby;
3) inoculation: get conidial powder 5g, medium 50kg,, mixed medium is positioned over and is cultivated in room, control temperature at 15-25 ℃, controlled humidity is at 75-85%, and incubation time is 22 days;
4) collection of culture is artificial golden cicada fungus and obtains thing;
5) resulting culture is dried under low temperature dehumidification condition (below 25 ℃, humidity below 45%), and be 36 hours drying time;
6) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
embodiment 6
1) the artificial golden cicada fungus conidial powder of collecting, is dried by the method for low temperature dehumidification (25 ℃ following, humidity 45% below), seals 0 ℃ of preservation (cryogenic freezing during long preservation), used time taking-up;
2) medium treatment: the wheat 50kg that peels off carries out 121 ℃ of high-temperature sterilizations (boiling) processing, standby;
3) inoculation: get conidial powder 5g, medium 50kg, is positioned over mixed medium to cultivate in room, controls temperature at 15-25 ℃, and controlled humidity is at 75-85%, and incubation time is 22 days;
4) collection of culture is artificial golden cicada fungus and obtains thing;
5) resulting culture is dried under low temperature dehumidification condition (below 25 ℃, humidity below 45%), and be 36 hours drying time;
6) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
embodiment 7
1) collect or wild golden cicada fungus that buying is arrived, select conidial powder more, color is whiter, by the method for low temperature dehumidification (20 ℃ below, humidity below 45%), be dried;
2) dried wild golden cicada fungus conidial powder is collected;
3) conidial powder after collection is in zero degree Refrigerator store (long preservation cryogenic freezing), and the used time takes out;
4) medium treatment: according to rice: dusty yeast 5:1 ratio altogether 50kg is mixed, then carry out 121 ℃ of high-temperature sterilizations (boiling) and process, standby;
5) inoculation: get conidial powder 5.0g, medium 50kg, mixed medium is positioned over to be cultivated in room, controls temperature at 15-25 ℃, and controlled humidity is at 65-75%, and incubation time is 22 days;
6) collection of culture is artificial golden cicada fungus, contains: coremium, conidial powder, medium and mycelia mixture;
7) resulting culture is dried under low temperature dehumidification condition (below 25 ℃, humidity below 45%), and be 24---48 hour drying time;
8) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
embodiment 8
1) the artificial golden cicada fungus conidial powder of collecting, is dried by the method for low temperature dehumidification (25 ℃ following, humidity 45% below), seals 0 ℃ of preservation (cryogenic freezing during long preservation), used time taking-up;
2) medium treatment: rice 50kg carries out 121 ℃ of high-temperature sterilizations (boiling) processing, standby;
3) inoculation: get conidial powder 5g, medium 50kg, is positioned over mixed medium to cultivate in room, controls temperature at 15-25 ℃, and controlled humidity is at 75-85%, and incubation time is 22 days;
4) collection of culture is artificial golden cicada fungus and obtains thing;
5) resulting culture is dried under low temperature dehumidification condition (below 25 ℃, humidity below 45%), and be 36 hours drying time;
6) dried cicada fungus packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Claims (3)
1. an artificial golden cicada fungus production method, is characterized in that, comprising:
1) by wild golden cicada fungus low temperature dehumidification, temperature is below 25 ℃, and humidity, below 45%, is dried, and wild golden cicada fungus selects that conidial powder is more, color is whiter;
2) dried wild golden cicada fungus conidial powder is collected;
3) conidial powder after collection is in zero degree Refrigerator store, and the used time takes;
4) medium treatment: according to wheat or the rice of peeling off: dusty yeast be take the ratio that weight ratio is 5-10:1 and mixed, then carries out high-temperature sterilization processing, standby;
5) inoculation: according to conidial powder: medium be take weight ratio as 1:10000 ratio mixes, and mixed medium is positioned over and is cultivated in room, controls temperature at 15-25 ℃, and controlled humidity is at 65-85%, and incubation time is 20-30 days;
6) collection of culture is artificial golden cicada fungus and obtains thing, contains: coremium, conidial powder, medium and mycelia mixture.
2. a kind of artificial golden cicada fungus production method according to claim 1, is characterized in that, in step 4) medium treatment, with 121 ℃ of high-temperature sterilizations, processes.
3. a kind of artificial golden cicada fungus production method according to claim 1, is characterized in that: resulting golden cicada fungus can be used as food, health products, medicine use, or uses as the auxiliary material of food, health products, medicine.
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Cited By (10)
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| CN103947455A (en) * | 2014-04-28 | 2014-07-30 | 吴益婷 | Cordyceps cicadae incubator |
| CN103947457A (en) * | 2014-04-28 | 2014-07-30 | 吴益婷 | Golden cicada inoculation pricking device |
| CN105993584A (en) * | 2015-03-26 | 2016-10-12 | 浙江泛亚生物医药股份有限公司 | Artificial culturing method for magnesium-rich cordyceps sobolifera |
| CN106387600A (en) * | 2016-08-29 | 2017-02-15 | 金寨县丰乐园农业科技开发有限公司 | Isaria cicadae miquel processing and storing method |
| CN106912557A (en) * | 2015-12-24 | 2017-07-04 | 浙江泛亚生物医药股份有限公司 | A kind of drying means of cicada fungus fructification |
| WO2017202293A1 (en) * | 2016-05-23 | 2017-11-30 | 浙江泛亚生物医药股份有限公司 | Artificial cultivation method for cordyceps sobolifera |
| CN107518394A (en) * | 2017-10-18 | 2017-12-29 | 湖州新驰医药科技有限公司 | A kind of production method of golden cicada flower fermentation instant powder |
| CN107699539A (en) * | 2017-10-18 | 2018-02-16 | 湖州新驰医药科技有限公司 | A kind of collection method of Periostracum cicadae conidia powder |
| CN107841421A (en) * | 2017-12-21 | 2018-03-27 | 陈国欣 | A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor |
| CN110226459A (en) * | 2019-07-04 | 2019-09-13 | 福建农林大学 | A kind of cultural method of cicada fungus conidia powder |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947455A (en) * | 2014-04-28 | 2014-07-30 | 吴益婷 | Cordyceps cicadae incubator |
| CN103947457A (en) * | 2014-04-28 | 2014-07-30 | 吴益婷 | Golden cicada inoculation pricking device |
| CN103947455B (en) * | 2014-04-28 | 2016-01-13 | 吴益婷 | Gold cicada fungus incubator |
| CN105993584A (en) * | 2015-03-26 | 2016-10-12 | 浙江泛亚生物医药股份有限公司 | Artificial culturing method for magnesium-rich cordyceps sobolifera |
| CN105993584B (en) * | 2015-03-26 | 2019-09-10 | 浙江泛亚生物医药股份有限公司 | A kind of artificial culture method of rich magnesium cicada fungus |
| CN106912557A (en) * | 2015-12-24 | 2017-07-04 | 浙江泛亚生物医药股份有限公司 | A kind of drying means of cicada fungus fructification |
| WO2017202293A1 (en) * | 2016-05-23 | 2017-11-30 | 浙江泛亚生物医药股份有限公司 | Artificial cultivation method for cordyceps sobolifera |
| CN106387600A (en) * | 2016-08-29 | 2017-02-15 | 金寨县丰乐园农业科技开发有限公司 | Isaria cicadae miquel processing and storing method |
| CN107518394A (en) * | 2017-10-18 | 2017-12-29 | 湖州新驰医药科技有限公司 | A kind of production method of golden cicada flower fermentation instant powder |
| CN107699539A (en) * | 2017-10-18 | 2018-02-16 | 湖州新驰医药科技有限公司 | A kind of collection method of Periostracum cicadae conidia powder |
| CN107699539B (en) * | 2017-10-18 | 2021-07-09 | 湖州新驰医药科技有限公司 | Method for collecting cordyceps cicadae miq spore powder |
| CN107841421A (en) * | 2017-12-21 | 2018-03-27 | 陈国欣 | A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor |
| CN110226459A (en) * | 2019-07-04 | 2019-09-13 | 福建农林大学 | A kind of cultural method of cicada fungus conidia powder |
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