CN104446919A - Culture substrate for shed-type artificial cultivation mellea armillaria sporophore and cultivation method thereof - Google Patents

Culture substrate for shed-type artificial cultivation mellea armillaria sporophore and cultivation method thereof Download PDF

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Publication number
CN104446919A
CN104446919A CN201410650238.9A CN201410650238A CN104446919A CN 104446919 A CN104446919 A CN 104446919A CN 201410650238 A CN201410650238 A CN 201410650238A CN 104446919 A CN104446919 A CN 104446919A
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cultivation
mushroom
sporophore
mellea
mellea armillaria
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CN201410650238.9A
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CN104446919B (en
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崔巍
郑焕春
施汉钰
栾泰龙
李淑玲
付静
越建伟
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HEILONGJIANG MUDANJIANG INSTITUTE OF FORESTRY
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HEILONGJIANG MUDANJIANG INSTITUTE OF FORESTRY
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a culture substrate for shed-type artificial cultivation mellea armillaria sporophore and a cultivation method thereof and belongs to the technical field of cultivation of mellea armillaria sporophore. By the culture substrate, the cultivation of mellea armillaria sporophore in a non-forestry land is achieved and the culture substrate comprises 40-41% of sawdust, 40-41% of auricularia auricula fungi residues, 14-16% of wheat bran, 0.8-1% of soybean flour, 0.8-1% of glucose, 0.8-1% of gypsum, 0.3-0.5% of lime, 0.4-0.5% of protein peptide, 0.03% of biotamin, 0.03% of plant ash and 0.02% of MgSO4. The cultivation method comprises the following steps of preparation of fungi bags, inoculating, mycelia-growing management and fruiting management. In a physical manner, a mellea armillaria sporophore button-inducing agent is prepared from artificial cultivation wastes of auricularia auricular so that the formation of buttons is promoted and the difficult problem that mellea armillaria sporophore can not be fruited in the non-forestry land is solved. By adopting auricularia auricular waste residues as raw materials in the non-forestry shed, the destruction and consumption of forest land or fungus materials to forest resources are avoided and the problem of the environmental pollution caused by the auricularia auricular waste residues is also solved. In order to realize the aim of stable mellea armillaria sporophore fruiting, the mellea armillaria sporophore button-inducing agent is researched and the technical difficult problem of the artificial button induction is solved.

Description

The cultivation medium formula of a kind of canopy formula artificial culture hazel mushroom and cultivating method
Technical field
The present invention relates to hazel mushroom cultivation technique, to realize the cultivation of hazel mushroom non-forest land.
Background technology
Hazel mushroom formal name used at school honey mushroom ( armillariella mellea), belong to the mushroom of Basidiomycotina, Agaricales, Tricholomataceae, Armillaria.All there is distribution in different continents, be mainly distributed in the provinces and regions such as Heilungkiang, Jilin, Liaoning, the Inner Mongol in China.Hazel mushroom is a kind of summer, generation in autumn, energy facultative parasitism is in multiple woody, herbal famous dietotherapeutic bacterium, the base portion of growing thickly in veteran or dead tree more, also can parasitize and live on tree, also can raised growth on thicket and meadow, its delicious flavour, nutritious, it is one of northeast wild mushroom deeply popular to people, be rich in the nutritions such as trace element, protein, carotene, vitamins C such as a large amount of calcium, phosphorus, iron, be tens times of general vegetables, be classified as a based food by some developed countries.Show through a large amount of experimental studies, its mycelium and fermented liquid all have dysfunction of nervous regulation and the brain defencive functions such as stronger hypnosis, anticonvulsion, anti-hypoxia, also have and increase the heart, brain vessel blood amount, the physiologically actives such as enhancing body immunoloregulation function.Through the unremitting effort of researcher and medicine manufacture, the preparations such as NAOXINSHU oral liquid, honey mushroom sheet, honey mushroom syrup have come out and have promoted the well-being of mankind.Hazel mushroom is not only a kind of edible mushrooms, but also has very high health care and pharmaceutical use, has very high research and development and is worth.
The state that the exploitation of wildness hazel-mushroom resource are in substantially " self-sow, freely pluck, autonomous transaction ", along with the forest reserves with vegetation deterioration is serious and the predatory collection of people, output is declining year by year.Current artificial culture is forest land cultivation or bacterium material (stub, juggle) planting type mainly.Hazel mushroom is the pathogeny bacterium of tree root maize ear rot; multiple deciduous tree and softwood tree can be infected; multiple softwood tree and deciduous tree root rot can be caused; its host plant reaches more than 300 genus; this disease mainly occurs in butt and root, the middle treelet of main harm life in 1 ~ 20 year, and usual treelet morbidity 2 ~ 3 years just can be lethal; strong age and aged tree then can delay about 10 years even longer, be one of object of wood protection preferential defense.
General tame edible mushrooms is all saprophytic microorganism, as long as mycelial growth grows physiological maturity, mycelia kink forms former base, developing into reproductive growth, finally obtaining sporophore from nourishing and growing.Hazel mushroom is parasitic saprophytic microorganism of holding concurrently, and nourishes and grows the same with general saprophytic microorganism, but to reaching maturity, mycelia kink forms shoestring, and shoestring is suitable for situation growth or shoestring in nutrition and growing environment, not easily forms former base.Current hazel mushroom artificial culture is Proterozoic cultivation (utilizing the shoestring of Proterozoic hazel mushroom) or bacterium material (stub, juggle) planting type mainly, is all imitative wild pattern.Hazel mushroom is different from other edible bacteriums, and comparatively strong to the dependency of wild environment, artificial booth cultivation never makes a breakthrough technically.And forest land expands numerous and cultivation is very big to devastation, realizing canopy room artificial culture can meet people's demand growing to hazel mushroom, can avoid again the injury to forest, has earth shaking economic worth.
Summary of the invention
Technical problem to be solved by this invention is: the formula and the cultivating method that provide a kind of canopy formula artificial culture hazel mushroom, to break away from the dependence of artificial culture to physical environment.
Technical solution of the present invention is: the cultivation medium formula of a kind of canopy formula artificial culture hazel mushroom, by mass percentage, by wood chip 40 ~ 41%, Auricularia fungus slag 40 ~ 41%, wheat bran 14 ~ 16%, bean powder 0.8 ~ 1%, glucose 0.8 ~ 1%, gypsum 0.8 ~ 1%, lime 0.3 ~ 0.5%, protein peptide 0.4 ~ 0.5%, Huolisu 0.03%, plant ash 0.03%, MgSO 40.02% is formed.
Another object of the present invention is to provide the cultivating method of a kind of canopy formula artificial culture hazel mushroom, comprises the following steps:
(1), bacterium bag makes: according to aforementioned formula weighing, water transfer, to water ratio 70%, stirs, and bag seals, normal-pressure sterilization 10 hours;
(2), inoculate: connect bacterium according to aseptic technique;
(3), hair tube reason: culture temperature is 23 DEG C ~ 27 DEG C, and half-light is cultivated 60 ~ 70 days;
(4), management of producing mushroom: adopt single upright emission type Ways of fruiting, thermal stimulation 3 days, when bacterium bag surface annesl is chocolate, black shoestring sprays and urges flower bud agent after occurring, promote that shoestring is divided into former base, after former base develops into mushroom flower bud, relative air humidity brings up to 65 ~ 70%, and temperature controls 18 DEG C ~ 22 DEG C, after ripening in 7 ~ 10 days, gathers.
Technique effect of the present invention is: by physics mode, utilizes artificial culture auricularia auriculajudae waste material, and preparation hazel mushroom urges flower bud agent, promotes that mushroom flower bud is formed, and solves a hazel mushroom non-forest land fruiting difficult problem.The present invention adopts canopy formula fruiting, breaches and utilizes the saprophytic fruiting pattern of Proterozoic shoestring and the parasitic fruiting pattern of bacterium material.Adopt auricularia auriculajudae Waste compost to be raw material, both avoided forest land or bacterium material to the destruction of the forest reserves and consumption, and made again auricularia auriculajudae Waste compost obtain recycling.Hazel mushroom urges the development of flower bud agent, solves a difficult problem for hazel mushroom fruiting instability, solves and manually urge flower bud technical barrier.
Embodiment
Embodiment 1:
1, cultivation medium formula
Auricularia fungus slag 40%, wheat bran 15%, bean powder 1%, glucose 1%, gypsum 1%, lime 0.6%, protein peptide 0.5%, Huolisu 0.03%, plant ash 0.03%, MgSO 40.02%, all the other wood chips.
2, bacterium bag makes
According to formula weighing, water transfer, to water ratio 70%, stirs, and bag seals, normal-pressure sterilization 10 hours.In the environment cools of cleaning after sterilizing.
3, inoculate
Select moderate, the not aging bacterial classification of cell age, adopt inoculation tank or transfer room to connect bacterium in strict accordance with aseptic technique.
4, hair tube reason
Culture temperature is 25 DEG C ± 2 DEG C, and half-light is cultivated 60 ~ 70 days.Culture temperature preferably 25 DEG C.
5, management of producing mushroom
Adopt single upright emission type Ways of fruiting, thermal stimulation three days.When bacterium bag surface annesl is chocolate, after black shoestring occurs, spray and urge flower bud agent (the plain 33ppm of uranium energy, protein peptide 3%, glucose 1%, potassium primary phosphate 0.03%, magnesium sulfate 0.02%), promote that shoestring is divided into former base.After former base develops into mushroom flower bud, to space water spray, relative air humidity brings up to 65 ~ 70%, and temperature controls between 18 DEG C ~ 22 DEG C, and after ripening in 7 ~ 10 days, gathers.Thermal stimulation temperature is 5 DEG C ~ 10 DEG C.
The present invention uses Auricularia fungus slag wood fibre after fungus cultivation to become thoroughly decomposed decomposition, and hazel mushroom mycelium could be sprouted in field planting; Auxiliary material uses protein peptide, strengthening nitrogen element; Plant ash regulates potential of hydrogen, increases potassium element; The each Equilibrium of Trace Elements of Huolisu.
Uranium can element be one of foliage fertilizer that current field-crop effect is stronger, is more than 10 times of common foliage fertilizer.There is abrupt release huge energy, immerse plant and root system, improve crop defense mechanism, optimize plant gene, promote cellular plasm flowing, cut off germ biologic chain.This programme is urged in flower bud agent edible mushrooms first and is used uranium energy element, uranium energy fission factor can be distributed widely in material fast, activate the activity of multiple enzyme, transfer rapidly the fission of favourable edible mushrooms gene and expand the quick division growth of the factor, accelerate mycelia division ripe and promote that former base is formed.
Other edible mushroom bacterium pack bag water content substantially 60 ~ 65%, and the hazel mushroom shoestring later stage cannot water filling and work of water sprinkling for better material moisture, can not cause hazel mushroom.Hazel mushroom of the present invention needs after first shoestring appears in manual-induced hazel mushroom, again to bring out and urge flower bud fruiting, and bacterium packed bag and directly controlled at 70% water ratio early stage, was applicable to later stage hazel mushroom shoestring growth and fruiting.The present invention takes physics urge flower bud and urge flower bud agent to be combined, and utilizes thermal stimulation hazel mushroom bacterium bag annesl and forms rhzomorph, utilizes and urges flower bud agent to stimulate former base to be formed.Wherein uranium can promote shoestring Tom Clancy Splinter Cell by element, and protein peptide, glucose, potassium primary phosphate and magnesium sulfate have additional nutrients, and accelerate shoestring Tom Clancy Splinter Cell, finally impel shoestring to be differentiated to form former base.

Claims (5)

1. a cultivation medium formula for canopy formula artificial culture hazel mushroom, is characterized in that, by mass percentage, by wood chip 40 ~ 41%, Auricularia fungus slag 40 ~ 41%, wheat bran 14 ~ 16%, bean powder 0.8 ~ 1%, glucose 0.8 ~ 1%, gypsum 0.8 ~ 1%, lime 0.3 ~ 0.5%, protein peptide 0.4 ~ 0.5%, Huolisu 0.03%, plant ash 0.03%, MgSO 40.02% is formed.
2. a cultivating method for canopy formula artificial culture hazel mushroom, is characterized in that, comprise the following steps:
(1), bacterium bag makes: according to weighing of filling a prescription described in claim 1, water transfer, to water ratio 70%, stirs, and bag seals, normal-pressure sterilization 10 hours;
(2), inoculate: connect bacterium according to aseptic technique;
(3), hair tube reason: culture temperature is 23 DEG C ~ 27 DEG C, and half-light is cultivated 60 ~ 70 days;
(4), management of producing mushroom: thermal stimulation 3 days, when bacterium bag surface annesl is chocolate, after black shoestring occurs, spray and urge flower bud agent, promote that shoestring is divided into former base, after former base develops into mushroom flower bud, relative air humidity brings up to 65 ~ 70%, temperature controls 18 DEG C ~ 22 DEG C, and after ripening in 7 ~ 10 days, gathers.
3. cultivating method as claimed in claim 2, it is characterized in that, described step (3) culture temperature is 25 DEG C.
4. cultivating method as claimed in claim 2, it is characterized in that, described step (4) thermal stimulation temperature is 5 DEG C ~ 10 DEG C.
5. cultivating method as claimed in claim 2, it is characterized in that, described step (4) adopts single upright emission type Ways of fruiting.
CN201410650238.9A 2014-11-17 2014-11-17 The cultivation medium formula of a kind of canopy formula artificial cultivation hazel mushroom and cultural method Active CN104446919B (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104996163A (en) * 2015-06-11 2015-10-28 临汾市尧都区仓禀实香菇种植专业合作社 Shiitake mushroom cultivation method
CN105917959A (en) * 2016-04-21 2016-09-07 哈尔滨天源工贸有限公司 Method for artificial soilless culture of large-cluster arimillaria mellea in four seasons
CN106358764A (en) * 2016-10-18 2017-02-01 辽东学院 Wild arimillaria mellea artificial domesticating and cultivating method
CN106416752A (en) * 2016-09-30 2017-02-22 朱良君 Artificial cultivation method for arimillaria mellea
CN106613317A (en) * 2016-10-18 2017-05-10 辽东学院 Fruiting induction method in mellea armillaria sporophore artificial cultivation
CN106688622A (en) * 2016-12-15 2017-05-24 新昌县奥而特农业科技有限公司 Culture medium formula of artificially cultivated armillaria mellea and cultivation method
CN111373979A (en) * 2020-04-21 2020-07-07 延安大学 Method for industrially cultivating pholiota nameko by using black fungus residues

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101343195A (en) * 2007-10-13 2009-01-14 鲁东大学 Artificial cultivation method for halimasch sporophore, culture medium and uses thereof
CN102742452A (en) * 2011-04-19 2012-10-24 重庆市药物种植研究所 Manufacture method of corncob halimasch production seeds
JP5509403B2 (en) * 2008-02-29 2014-06-04 独立行政法人森林総合研究所 Maitake Cultivation Method and Maitake Yield Increaser

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101343195A (en) * 2007-10-13 2009-01-14 鲁东大学 Artificial cultivation method for halimasch sporophore, culture medium and uses thereof
JP5509403B2 (en) * 2008-02-29 2014-06-04 独立行政法人森林総合研究所 Maitake Cultivation Method and Maitake Yield Increaser
CN102742452A (en) * 2011-04-19 2012-10-24 重庆市药物种植研究所 Manufacture method of corncob halimasch production seeds

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104996163A (en) * 2015-06-11 2015-10-28 临汾市尧都区仓禀实香菇种植专业合作社 Shiitake mushroom cultivation method
CN105917959A (en) * 2016-04-21 2016-09-07 哈尔滨天源工贸有限公司 Method for artificial soilless culture of large-cluster arimillaria mellea in four seasons
CN106416752A (en) * 2016-09-30 2017-02-22 朱良君 Artificial cultivation method for arimillaria mellea
CN106358764A (en) * 2016-10-18 2017-02-01 辽东学院 Wild arimillaria mellea artificial domesticating and cultivating method
CN106613317A (en) * 2016-10-18 2017-05-10 辽东学院 Fruiting induction method in mellea armillaria sporophore artificial cultivation
CN106688622A (en) * 2016-12-15 2017-05-24 新昌县奥而特农业科技有限公司 Culture medium formula of artificially cultivated armillaria mellea and cultivation method
CN111373979A (en) * 2020-04-21 2020-07-07 延安大学 Method for industrially cultivating pholiota nameko by using black fungus residues

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