CN105917959A - Method for artificial soilless culture of large-cluster arimillaria mellea in four seasons - Google Patents
Method for artificial soilless culture of large-cluster arimillaria mellea in four seasons Download PDFInfo
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- CN105917959A CN105917959A CN201610249954.5A CN201610249954A CN105917959A CN 105917959 A CN105917959 A CN 105917959A CN 201610249954 A CN201610249954 A CN 201610249954A CN 105917959 A CN105917959 A CN 105917959A
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- mellea
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- arimillaria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention discloses a method for artificial soilless culture of large-cluster arimillaria mellea in four seasons, and relates to a method for soilless culture of arimillaria mellea. The invention aims at solving the technical problems that wild arimillaria mellea resources are decreased gradually and cannot meet market and consumer demands due to a relatively low natural production of the arimillaria mellea and disordered picking of wild arimillaria mellea by people. The method comprises the following steps: 1, selecting parent source strains; 2, selecting first-class strains; 3, selecting second-class strains; and 4, performing disinfection and sterilization in a greenhouse, then placing fungus bags treated in the step 3 on lifting ropes or storage racks, and performing culture for 25-30 days under the condition that the humidity is 85-90%, the daytime temperature is 20-24 DEG C, and the night temperature is 10-14 DEG C, thereby obtaining the large-cluster arimillaria mellea. The large-cluster arimillaria mellea cultured by adoption of the method disclosed by the invention can be picked for two to three times by one culture. The technical problem that the wild arimillaria mellea resources are decreased gradually due to the relatively low natural production of the arimillaria mellea and disordered picking of wild arimillaria mellea by people is solved. The method disclosed by the invention belongs to the field of arimillaria mellea culture.
Description
Technical field
A kind of method that the present invention relates to soilless culture Armillariella mellea.
Background technology
Armillariella mellea is the sporophore of Mycophyta door fungus Armillaria mellea.Wildness hazel-mushroom is mainly distributed on mountain forest district, Heilungkiang.By people
It is referred to as " delicacy from mountain ".Sliding tender tasty and refreshing, delicious flavour, nutritious, Armillariella mellea contains several amino acids and the vitamin of needed by human,
The nutritions such as protein, carotene, vitamin C, are tens times of general vegetable, often eat and can strengthen human body
Immunity, the effect of prolonging life etc. that having makes light of one's life by commiting suicide, it is classified as a based food by country.Armillariella mellea is umbrella shape, faint yellow, dry rear sepia.
Annual 7～be grown in August coniferous and broadleaved tree butt portion, fall wood and be embedded on the branch in soil.General how raw at Middle hills
Zhen Chaigang on, so gain the name " Armillariella mellea ".Wildness hazel-mushroom is one of distinctive delicacy from mountain in Northeast China, is very difficult to artificial culture
One of edible fungi.Owing to current Armillariella mellea natural production is relatively low, the people's unordered harvesting to wildness hazel-mushroom, cause wildness hazel-mushroom
Resource reduces increasingly, can not meet the demand of market and consumption, and my company repeatedly tests through more than ten years and takes pains to foster, and grasps
The professional technique of canopy room four seasons artificial culture big pier Armillariella mellea, is the Armillariella mellea kind of a kind of stable yields, high-quality.Allow people 1 year four
Ji Douneng has fresh Armillariella mellea.
Summary of the invention
The invention aims to solve Armillariella mellea natural production relatively low, the people's unordered harvesting to wildness hazel-mushroom, cause wild
Armillariella mellea resource reduces increasingly, can not meet the technical problem of market and consumption demand, it is provided that a kind of four seasons manually cultivate without scholar
The method of big pier Armillariella mellea.
The method of the four seasons artificial soilless culture big pier Armillariella mellea is as follows:
One, source of parents strain:
Choose the shoestring of natural Armillariella mellea white, take its tender part of children of most advanced and sophisticated 1-2 centimetre, after surface sterilization, scrape with tweezers
Fall the sheath of shoestring to the color marrow that shows money or valuables one carries unintentionally, the segment that picking is 5 millimeters, be placed in culture medium, and under the conditions of 25-26 DEG C
Cultivate 12-15 days, obtain source of parents strain；
Described culture medium by 50 grams of wood flours, 20 grams of Testa Tritici, 200 grams of Rhizoma Solani tuber osis, 20 grams of sucrose, 20 grams of agar, 0.5 gram
Potassium dihydrogen phosphate, 0.3 gram of magnesium sulfate and 1000 grams of water compositions；
Two, first class inoculum:
From the source of parents strain of step one gained, select sturdy white shoestring, after surface sterilization, take its young tender part, with connecing bacterium
Bacterium wiped off by pin and rope is placed on bacterium bag plating medium, cultivates 20-25 days, obtain first class inoculum under the conditions of 25-26 DEG C；
Described plating medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder
Form with 130-150 part water, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Three, second class inoculum:
Choose the first class inoculum of sturdy white shoestring, after surface sterilization, take its young tender part, with connecing acicula, shoestring is placed in
On bacterium bag plating medium, cultivate 25-30 days under the conditions of 25-26 DEG C, obtain second class inoculum；
Described plating medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder
Form with 130-150 part water, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Or described plating medium is by weight by 84 parts of corn cobs, 15 parts of Testa Tritici, 1 part of Gypsum Fibrosum, 110-130
Part water composition；
Four, by canopy indoor sterilizing, then the bacterium bag processed through step 3 is put on lifting rope or shelf, in humidity
For 85%-90%, day temperature be 20-24 DEG C, evening temperature be 10-14 DEG C under conditions of cultivate 25-30 days, obtain greatly
Pier Armillariella mellea.
Use the big pier Armillariella mellea of the inventive method cultivation, once cultivate and can adopt two to three batches.Solve Armillariella mellea natural production relatively low,
The people's unordered harvesting to wildness hazel-mushroom, the technical problem causing wildness hazel-mushroom resource increasingly to reduce, meet market and consumption
Demand.
Detailed description of the invention
Technical solution of the present invention is not limited to act detailed description of the invention set forth below, and also include between each detailed description of the invention is any
Combination.
Detailed description of the invention one: in present embodiment, the method for the four seasons artificial soilless culture big pier Armillariella mellea is as follows:
One, source of parents strain:
Choose the shoestring of natural Armillariella mellea white, take its tender part of children of most advanced and sophisticated 1-2 centimetre, after surface sterilization, scrape with tweezers
Fall the sheath of shoestring to the color marrow that shows money or valuables one carries unintentionally, the segment that picking is 5 millimeters, be placed in culture medium, and under the conditions of 25-26 DEG C
Cultivate 12-15 days, obtain source of parents strain；
Described culture medium by 50 grams of wood flours, 20 grams of Testa Tritici, 200 grams of Rhizoma Solani tuber osis, 20 grams of sucrose, 20 grams of agar, 0.5 gram
Potassium dihydrogen phosphate, 0.3 gram of magnesium sulfate and 1000 grams of water compositions；
Two, first class inoculum:
From the source of parents strain of step one gained, select sturdy white shoestring, after surface sterilization, take its young tender part, with connecing bacterium
Bacterium wiped off by pin and rope is placed on bacterium bag plating medium, cultivates 20-25 days, obtain first class inoculum under the conditions of 25-26 DEG C；
Described plating medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder
Form with 130-150 part water, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Three, second class inoculum:
Choose the first class inoculum of sturdy white shoestring, after surface sterilization, take its young tender part, with connecing acicula, shoestring is placed in
On bacterium bag plating medium, cultivate 25-30 days under the conditions of 25-26 DEG C, obtain second class inoculum；
Described plating medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder
Form with 130-150 part water, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Or described plating medium is by weight by 84 parts of corn cobs, 15 parts of Testa Tritici, 1 part of Gypsum Fibrosum, 110-130
Part water composition；
Four, by canopy indoor sterilizing, then the bacterium bag processed through step 3 is put on lifting rope or shelf, in humidity
For 85%-90%, day temperature be 20-24 DEG C, evening temperature be 10-14 DEG C under conditions of cultivate 25-30 days, obtain greatly
Pier Armillariella mellea.
Detailed description of the invention two: present embodiment wood flour described in step one unlike detailed description of the invention one is Xylosmae japonici bits
Or Semen coryli heterophyllae bits (hardwood).Other is identical with detailed description of the invention one.
Detailed description of the invention three: in 25 DEG C in present embodiment step one unlike one of detailed description of the invention one or two
Under the conditions of cultivate 13 days, obtain source of parents strain.Other is identical with one of detailed description of the invention one or two.
Detailed description of the invention four: in 26 DEG C in present embodiment step 2 unlike one of detailed description of the invention one to three
Under the conditions of cultivate 23 days, obtain first class inoculum.Other is identical with one of detailed description of the invention one to three.
Detailed description of the invention five: in 26 DEG C in present embodiment step 2 unlike one of detailed description of the invention one to four
Under the conditions of cultivate 28 days, obtain second class inoculum.Other is identical with one of detailed description of the invention one to four.
Detailed description of the invention six: present embodiment is putting down described in step 2 unlike one of detailed description of the invention one to five
Face culture medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder and 140 parts of water groups
Become.Other is identical with one of detailed description of the invention one to five.
Detailed description of the invention seven: in present embodiment step 3 unlike one of detailed description of the invention one to six in
Cultivate 25-30 days under the conditions of 25-30 DEG C, Armillariella mellea of can gathering.Other is identical with one of detailed description of the invention one to six.
Detailed description of the invention eight: present embodiment is putting down described in step 3 unlike one of detailed description of the invention one to seven
Face culture medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder and 140 parts of water groups
Become.Other is identical with one of detailed description of the invention one to seven.
Detailed description of the invention nine: in present embodiment step 4 unlike one of detailed description of the invention one to eight, canopy is indoor
Sterilization, then puts on lifting rope or shelf by the bacterium bag processed through step 3, humidity be 88%, temperature on daytime
Degree is 22 DEG C, evening temperature is cultivated 28 days under conditions of being 13 DEG C.Other is identical with one of detailed description of the invention one to eight.
Use following experimental verification effect of the present invention:
Experiment one:
The method of the four seasons artificial soilless culture big pier Armillariella mellea is as follows:
One, source of parents strain:
Choose the shoestring of sturdy white in natural Armillariella mellea and take its young tender part, take its tender part of children of most advanced and sophisticated 2 centimetres, through surface
After sterilization, wipe the sheath of shoestring off to the color marrow that shows money or valuables one carries unintentionally, the segment that picking is 5 millimeters with tweezers, be placed in culture medium, and
Cultivate 12 days under the conditions of 25 DEG C, obtain source of parents strain；
Described culture medium by 50 grams of wood flours, 20 grams of Testa Tritici, 200 grams of Rhizoma Solani tuber osis, 20 grams of sucrose, 20 grams of agar, 0.5 gram
Potassium dihydrogen phosphate, 0.3 gram of magnesium sulfate and 1000 grams of water compositions；
Two, first class inoculum:
From the source of parents strain of step one gained, select sturdy white shoestring, after surface sterilization, take its young tender part, with connecing bacterium
Pin is wiped shoestring off and is placed on bacterium bag plating medium, cultivates 20 days, obtain first class inoculum under the conditions of 25 DEG C；
Described plating medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder
Form with 130 parts of water, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Three, second class inoculum:
Choose the first class inoculum of sturdy white shoestring, after surface sterilization, take its young tender part, with connecing acicula, shoestring is placed in
On bacterium bag plating medium, cultivate 25 days under the conditions of 25 DEG C, obtain second class inoculum；
Described plating medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder
Form with 130 parts of water, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Or described plating medium is by weight by 84 parts of corn cobs, 15 parts of Testa Tritici, 1 part of Gypsum Fibrosum, 110 parts of water
Composition；
Four, by canopy indoor sterilizing, then the bacterium bag processed through step 3 is put on lifting rope or shelf, in humidity
Be 85%, day temperature be 20 DEG C, evening temperature cultivate mushroom thunder seen from 25 days, then after 7 days under conditions of being 10 DEG C
Can gather, obtain big pier Armillariella mellea.Two to three batches can be adopted.
Experiment two:
The method of the four seasons artificial soilless culture big pier Armillariella mellea is as follows:
One, source of parents strain:
Choose the shoestring of natural Armillariella mellea white, take its tender part of children of most advanced and sophisticated 2 centimetres, after surface sterilization, wipe off with tweezers
The sheath of shoestring, to the color marrow that shows money or valuables one carries unintentionally, the segment that picking is 5 millimeters, is placed in culture medium, and cultivates under the conditions of 26 DEG C
13 days, obtain source of parents strain；
Described culture medium by 50 grams of wood flours, 20 grams of Testa Tritici, 200 grams of Rhizoma Solani tuber osis, 20 grams of sucrose, 20 grams of agar, 0.5 gram
Potassium dihydrogen phosphate, 0.3 gram of magnesium sulfate and 1000 grams of water compositions；
Two, first class inoculum:
From the source of parents strain of step one gained, select sturdy white shoestring, after surface sterilization, take its young tender part, with connecing bacterium
Pin is wiped shoestring off and is placed on bacterium bag plating medium, cultivates 25 days, obtain first class inoculum under the conditions of 26 DEG C；
Described plating medium is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder
Form with 150 parts of water, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Three, second class inoculum:
Choose the first class inoculum of sturdy white shoestring, after surface sterilization, take its young tender part, with connecing acicula, shoestring is placed in
On bacterium bag plating medium, cultivate 28 days under the conditions of 26 DEG C, obtain second class inoculum；
Described plating medium is made up of 84 parts of corn cobs, 15 parts of Testa Tritici, 1 part of Gypsum Fibrosum, 110 parts of water by weight；
Four, by canopy indoor sterilizing, then the bacterium bag processed through step 3 is put on lifting rope or shelf, in humidity
For 85-90%, day temperature be 23 DEG C, evening temperature be 13 DEG C under conditions of cultivate mushroom thunder seen from 28 days, then 7 days
After can gather, obtain big pier Armillariella mellea.Two to three batches can be adopted.
Claims (9)
1. the method for the four seasons artificial soilless culture big pier Armillariella mellea, it is characterised in that: the side of the four seasons artificial soilless culture big pier Armillariella mellea
Method is as follows:
One, source of parents strain:
Choose the shoestring of natural Armillariella mellea white, take its tender part of children of most advanced and sophisticated 1-2 centimetre, after surface sterilization, scrape with tweezers
Fall the sheath of shoestring to the color marrow that shows money or valuables one carries unintentionally, the segment that picking is 5 millimeters, be placed in culture medium, and train under the conditions of 25-26 DEG C
Support 12-15 days, obtain source of parents strain；
Described culture medium is by 50 grams of wood flours, 20 grams of Testa Tritici, 200 grams of Rhizoma Solani tuber osis, 20 grams of sucrose, 20 grams of agar, 0.5 gram of phosphorus
Acid dihydride potassium, 0.3 gram of magnesium sulfate and 1000 grams of water compositions；
Two, first class inoculum:
From the source of parents strain of step one gained, select sturdy white shoestring, after surface sterilization, take its young tender part, with connecing bacterium
Bacterium wiped off by pin and rope is placed on bacterium bag plating medium, cultivates 20-25 days, obtain first class inoculum under the conditions of 25-26 DEG C；
Described plating medium by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder and
130-150 part water forms, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Three, second class inoculum:
Choose the first class inoculum of sturdy white shoestring, after surface sterilization, take its young tender part, with connecing acicula, shoestring is placed in
On bacterium bag plating medium, cultivate 25-30 days under the conditions of 25-26 DEG C, obtain second class inoculum；
Described plating medium by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder and
130-150 part water forms, and 1/3 bottom each bacterium bag is equipped with the hazel tree bar of a length of 2 centimetres；
Or described plating medium is by weight by 84 parts of corn cobs, 15 parts of Testa Tritici, 1 part of Gypsum Fibrosum, 110-130 parts
Water forms；
Four, by canopy indoor sterilizing, then the bacterium bag processed through step 3 is put on lifting rope or shelf, in humidity
For 85%-90%, day temperature be 20-24 DEG C, evening temperature be 10-14 DEG C under conditions of cultivate 25-30 days, obtain big pier
Armillariella mellea.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: institute in step one
Stating wood flour is Xylosmae japonici bits or Semen coryli heterophyllae bits.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: in step one in
Cultivate 13 days under the conditions of 25 DEG C, obtain source of parents strain.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: in step 2 in
Cultivate 23 days under the conditions of 26 DEG C, obtain first class inoculum.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: in step 2 in
Cultivate 28 days under the conditions of 26 DEG C, obtain second class inoculum.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: institute in step 2
The plating medium stated is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder and 140 parts
Water forms.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: in step 3 in
Cultivate 25-30 days under the conditions of 25-30 DEG C, Armillariella mellea of can gathering.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: institute in step 3
The plating medium stated is by weight by 78 parts of wood flours, 20 parts of Testa Tritici, 1 part of glucose powder, 1 part of Gypsum Fibrosum powder and 140 parts
Water forms.
The method of the four seasons artificial soilless culture big pier Armillariella mellea the most according to claim 1, it is characterised in that: will in step 4
Canopy indoor sterilizing, then puts on lifting rope or shelf by the bacterium bag processed through step 3, humidity be 88%, white
It temperature is 22 DEG C, evening temperature is cultivated 28 days under conditions of being 13 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613317A (en) * | 2016-10-18 | 2017-05-10 | 辽东学院 | Fruiting induction method in mellea armillaria sporophore artificial cultivation |
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