CN103598014B - A kind of production method of artificial isaria cicadae miq - Google Patents
A kind of production method of artificial isaria cicadae miq Download PDFInfo
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- CN103598014B CN103598014B CN201310597913.1A CN201310597913A CN103598014B CN 103598014 B CN103598014 B CN 103598014B CN 201310597913 A CN201310597913 A CN 201310597913A CN 103598014 B CN103598014 B CN 103598014B
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Abstract
The invention discloses the production method of a kind of artificial isaria cicadae miq, its technical parameter and culture medium condition etc. obtain through optimization for many years, parameters and Medium Proportion and working condition very scientific and precise, through verification experimental verification, produces and is capable of and can obtain high-quality artificial culture thing.The cultivated days of the present invention is shorter, and culture medium is rice, Semen Tritici aestivi common on market or is Semen Tritici aestivi, the mixture of yeast powder, and adjuvant is easy to get and Costco Wholesale is relatively low, can carry out industrialized production in a large number.Obtained Cordyceps cicadae Shing can use as food, health product, medicine, or uses as food, health product, the adjuvant of medicine.
Description
Technical field
The present invention relates to the production method of a kind of artificial isaria cicadae miq, belong to the artificial production technical field of medicine-food two-purpose fungus.
Background technology
Periostracum cicadae (CordycepsciecadaeShing) is also referred to as Cicadae bacterium Cordyceps sobolifera, be a kind of marvellous biological root with animal and plant feature be the larva body of Cicadae pupa or mountain Cicadae, flower is from single or 2-3 Cicadae larva head grows out, and about one-inch how long, branch of blooming from top.Researching and proposing according to relevant, Periostracum cicadae belongs to biological morbid state phenomenon, is the interdependent assembly of a kind of entomogenous fungi.Autumn arrives, and Cicadae pierces in soil, gradually becomes Cicadae pupa, parasitic by Cordyceps mushroom before emergence, when life spare part is suitable, begins to sprout into mycelium, absorbs the nutrition of polypide, and final polypide is occupied completely by mycelium and only remains the next body.In all things on earth recovery time, mycelium is converted into the perfect stage from the vegetative phase again, branch of gradually blooming from top, so Periostracum cicadae of gaining the name.
The wild Cordyceps cicadae Shing of existing market, there are three characteristic comparing distinctnesses: one is owing to it is the one of Cordyceps, certain analogy is had with tradition Cordyceps, price is carried out substantially according to the Price advisor of Cordyceps, finally likely can become second Cordyceps, major part consumer will be unable to bear the costliness of price；Two intensifications being as exploitation, wild Cordyceps cicadae Shing resource can reduce in a large number, will greatly limit range；Three contract systems being as country mountain region, the environmental pollution such as the production of bamboo product and soil, cause wild Cordyceps cicadae Shing pesticide residues to be greatly increased with the probability of the pollution of heavy metal.For meeting the demand in market, for mankind's health service, artificial culture Cordyceps cicadae Shing will have important value and realistic meaning.
Summary of the invention
It is an object of the invention to provide the production method of a kind of artificial isaria cicadae miq, have the artificial culture Cordyceps cicadae Shing cultivation technique that production cost is low, with short production cycle, this production method has been divided by following steps:
1) by wild Cordyceps cicadae Shing low temperature dehumidification, temperature is below 25 DEG C, and humidity, below 45%, is dried, and spore powder is more, color is whiter for the selection of wild Cordyceps cicadae Shing；
2) dried wild Cordyceps cicadae Shing spore powder is collected；
3) spore powder after collecting was taken in zero degree Refrigerator store, used time；
4) medium treatment: according to Semen Tritici aestivi or the rice of peeling off: the yeast powder ratio mixing with weight ratio as 5-10:1, then carry out high temperature sterilize process, standby；
5) inoculation: according to spore powder: mixed culture medium, with weight ratio for 1:10000 ratio mix homogeneously, is positioned in cultivation room by culture medium, and control temperature is at 15-25 DEG C, and control humidity is at 65-85%, and incubation time is 20-30 days；
6) collection of culture is artificial isaria cicadae miq and obtains thing, contains: coremium, spore powder, culture medium and mycelia mixture.
In step 4) medium treatment, process with 121 DEG C of high temperature sterilizes.
Obtained Cordyceps cicadae Shing can use as food, health product, medicine, or uses as food, health product, the adjuvant of medicine.
The artificial isaria cicadae miq production method technical parameter of the present invention and culture medium condition etc. obtain through optimization for many years, parameters and Medium Proportion and working condition very scientific and precise, through verification experimental verification, produce and be capable of and high-quality artificial culture thing can be obtained.The cultivated days of the present invention is shorter, and culture medium is rice, Semen Tritici aestivi common on market or is Semen Tritici aestivi, the mixture of yeast powder, and adjuvant is easy to get and Costco Wholesale is relatively low, can carry out industrialized production in a large number.
Detailed description of the invention
Embodiment
1
1) collect or the wild Cordyceps cicadae Shing purchased, select spore powder more, color whiter, be dried by the method for low temperature dehumidification (less than 20 DEG C, humidity less than 45%)；
2) dried wild Cordyceps cicadae Shing spore powder is collected；
3) spore powder after collecting took out in zero degree Refrigerator store (preserving freezing for a long time), used time；
4) medium treatment: according to Semen Tritici aestivi of peeling off: yeast powder 5:1 ratio 50kg mixing altogether, then carry out 121 DEG C of high temperature sterilizes (steaming and decocting) process, standby；
5) inoculation: take spore powder 5.0g, culture medium 50kg, mixed culture medium is positioned in cultivation room, and control temperature is at 15-25 DEG C, and control humidity is at 65-75%, and incubation time is 22 days；
6) collection of culture is artificial isaria cicadae miq, contains: coremium, spore powder, culture medium and mycelia mixture；
7) culture obtained by is dried under low temperature dehumidification condition (less than 25 DEG C, humidity less than 45%), and drying time is 24---48 hour；
8) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Embodiment
2
1) collect or the wild Cordyceps cicadae Shing purchased, select spore powder more, white color, be dried by the method for low temperature dehumidification (less than 25 DEG C, humidity less than 45%)；
2) dried wild Cordyceps cicadae Shing spore powder is collected；
3) spore powder after collecting is in zero degree Refrigerator store (freezing during long-term preservation), and the used time takes out；
4) medium treatment: according to Semen Tritici aestivi of peeling off: yeast powder 10:1 ratio 50kg mixing altogether, then carry out 121 DEG C of high temperature sterilizes (steaming and decocting) process
5) inoculation: take spore powder 5g, culture medium 50kg, is positioned over mixed culture medium in cultivation room, and control temperature is at 15-25 DEG C, and control humidity is at 75-85%, and incubation time is 20---30 days；
6) collection of culture is artificial isaria cicadae miq, contains: coremium, spore powder, culture medium and mycelia mixture；；
7) culture obtained by is dried under low temperature dehumidification condition (less than 25 DEG C, humidity less than 45%), and drying time is 24--48 hour；
8) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Embodiment
3
1) collect or the wild Cordyceps cicadae Shing purchased, select spore powder more, color whiter, be dried by the method for low temperature dehumidification (less than 25 DEG C, humidity less than 45%)；
2) dried wild Cordyceps cicadae Shing spore powder is collected；
3) spore powder after collecting is in zero degree Refrigerator store, and (the freezing during long-term preservation) used time takes out；
4) medium treatment: the Semen Tritici aestivi 50kg that peels off carries out 121 DEG C of high temperature sterilizes (steaming and decocting) and processes；
5) inoculation: take spore powder 5g, culture medium 50kg, is positioned over mixed culture medium in cultivation room, and control temperature is at 19-20 DEG C, and control humidity is at 75-85%, and incubation time is 25 days；
6) collection of culture is artificial isaria cicadae miq；
7) culture obtained by is dried under low temperature dehumidification condition (less than 20 DEG C, humidity less than 45%), and drying time is 36 hours；
8) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Embodiment
4
1) the artificial isaria cicadae miq spore powder collected, is dried by the method for low temperature dehumidification (less than 25 DEG C, humidity less than 45%), seals 0 DEG C of preservation, and (the freezing during long-term preservation) used time takes out；
2) medium treatment: according to Semen Tritici aestivi of peeling off: yeast powder 5:1 ratio altogether 50kg mixing, then carry out 121 DEG C of high temperature sterilizes (steaming and decocting) process, standby；
3) inoculation: take spore powder 5g, culture medium 50kg, is positioned over mixed culture medium in cultivation room, and control temperature is at 15-25 DEG C, and control humidity is at 65-75%, and incubation time is 22 days；
4) collection of culture is artificial isaria cicadae miq and obtains thing；
5) culture obtained by is dried under low temperature dehumidification condition (less than 25 DEG C, humidity less than 45%), and drying time is 36 hours；
6) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Embodiment
5
1) the artificial isaria cicadae miq spore powder collected, is dried by the method for low temperature dehumidification (less than 25 DEG C, humidity less than 45%), seals 0 DEG C of preservation, and the used time takes out；
2) medium treatment: according to Semen Tritici aestivi of peeling off: yeast powder 10:1 ratio altogether 50kg mixing, then carry out 121 DEG C of high temperature sterilizes (steaming and decocting) process, standby；
3) inoculation: take spore powder 5g, culture medium 50kg, mixed culture medium being positioned in cultivation room, control temperature at 15-25 DEG C, control humidity at 75-85%, incubation time is 22 days；
4) collection of culture is artificial isaria cicadae miq and obtains thing；
5) culture obtained by is dried under low temperature dehumidification condition (less than 25 DEG C, humidity less than 45%), and drying time is 36 hours；
6) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Embodiment
6
1) the artificial isaria cicadae miq spore powder collected, is dried by the method for low temperature dehumidification (less than 25 DEG C, humidity less than 45%), seals 0 DEG C of preservation (freezing during long-term preservation), and the used time takes out；
2) medium treatment: the Semen Tritici aestivi 50kg that peels off carry out 121 DEG C of high temperature sterilizes (steaming and decocting) process, standby；
3) inoculation: take spore powder 5g, culture medium 50kg, is positioned over mixed culture medium in cultivation room, and control temperature is at 15-25 DEG C, and control humidity is at 75-85%, and incubation time is 22 days；
4) collection of culture is artificial isaria cicadae miq and obtains thing；
5) culture obtained by is dried under low temperature dehumidification condition (less than 25 DEG C, humidity less than 45%), and drying time is 36 hours；
6) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Embodiment
7
1) collect or the wild Cordyceps cicadae Shing purchased, select spore powder more, color whiter, be dried by the method for low temperature dehumidification (less than 20 DEG C, humidity less than 45%)；
2) dried wild Cordyceps cicadae Shing spore powder is collected；
3) spore powder after collecting took out in zero degree Refrigerator store (preserving freezing for a long time), used time；
4) medium treatment: according to rice: yeast powder 5:1 ratio 50kg mixing altogether, then carry out 121 DEG C of high temperature sterilizes (steaming and decocting) process, standby；
5) inoculation: take spore powder 5.0g, culture medium 50kg, mixed culture medium is positioned in cultivation room, and control temperature is at 15-25 DEG C, and control humidity is at 65-75%, and incubation time is 22 days；
6) collection of culture is artificial isaria cicadae miq, contains: coremium, spore powder, culture medium and mycelia mixture；
7) culture obtained by is dried under low temperature dehumidification condition (less than 25 DEG C, humidity less than 45%), and drying time is 24---48 hour；
8) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Embodiment
8
1) the artificial isaria cicadae miq spore powder collected, is dried by the method for low temperature dehumidification (less than 25 DEG C, humidity less than 45%), seals 0 DEG C of preservation (freezing during long-term preservation), and the used time takes out；
2) medium treatment: rice 50kg carry out 121 DEG C of high temperature sterilizes (steaming and decocting) process, standby；
3) inoculation: take spore powder 5g, culture medium 50kg, is positioned over mixed culture medium in cultivation room, and control temperature is at 15-25 DEG C, and control humidity is at 75-85%, and incubation time is 22 days；
4) collection of culture is artificial isaria cicadae miq and obtains thing；
5) culture obtained by is dried under low temperature dehumidification condition (less than 25 DEG C, humidity less than 45%), and drying time is 36 hours；
6) dried Periostracum cicadae packs, and concentrates and carries out irradiation sterilization according to food irradiation dosage 10KGY, gets product.
Claims (3)
1. an artificial isaria cicadae miq production method, it is characterised in that including:
1) by wild Cordyceps cicadae Shing low temperature dehumidification, temperature is below 25 DEG C, and humidity, below 45%, is dried, and spore powder is more, color is whiter for the selection of wild Cordyceps cicadae Shing；
2) dried wild Cordyceps cicadae Shing spore powder is collected；
3) spore powder after collecting was taken in zero degree Refrigerator store, used time；
4) medium treatment: according to Semen Tritici aestivi or the rice of peeling off: the yeast powder ratio mixing with weight ratio as 5-10:1, then carry out high temperature sterilize process, standby；
5) inoculation: according to spore powder: mixed culture medium, with weight ratio for 1:10000 ratio mix homogeneously, is positioned in cultivation room by culture medium, and control temperature is at 15-25 DEG C, and control humidity is at 65-85%, and incubation time is 20-30 days；
6) collection of culture is artificial isaria cicadae miq and obtains thing, contains: coremium, spore powder, culture medium and mycelia mixture.
A kind of artificial isaria cicadae miq production method the most according to claim 1, it is characterised in that in step 4) medium treatment, process with 121 DEG C of high temperature sterilizes.
A kind of artificial isaria cicadae miq production method the most according to claim 1, it is characterised in that: obtained Cordyceps cicadae Shing uses as food, health product, medicine, or uses as food, health product, the adjuvant of medicine.
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| CN103947457A (en) * | 2014-04-28 | 2014-07-30 | 吴益婷 | Golden cicada inoculation pricking device |
| CN103947455B (en) * | 2014-04-28 | 2016-01-13 | 吴益婷 | Gold cicada fungus incubator |
| CN105993584B (en) * | 2015-03-26 | 2019-09-10 | 浙江泛亚生物医药股份有限公司 | A kind of artificial culture method of rich magnesium cicada fungus |
| CN106912557A (en) * | 2015-12-24 | 2017-07-04 | 浙江泛亚生物医药股份有限公司 | A kind of drying means of cicada fungus fructification |
| CN107409742B (en) * | 2016-05-23 | 2020-10-23 | 浙江泛亚生物医药股份有限公司 | Artificial culture method of cordyceps sobolifera |
| CN106387600A (en) * | 2016-08-29 | 2017-02-15 | 金寨县丰乐园农业科技开发有限公司 | Isaria cicadae miquel processing and storing method |
| CN107518394A (en) * | 2017-10-18 | 2017-12-29 | 湖州新驰医药科技有限公司 | A kind of production method of golden cicada flower fermentation instant powder |
| CN107699539B (en) * | 2017-10-18 | 2021-07-09 | 湖州新驰医药科技有限公司 | Method for collecting cordyceps cicadae miq spore powder |
| CN107841421A (en) * | 2017-12-21 | 2018-03-27 | 陈国欣 | A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor |
| CN110226459A (en) * | 2019-07-04 | 2019-09-13 | 福建农林大学 | A kind of cultural method of cicada fungus conidia powder |
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