CN107841421A - A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor - Google Patents
A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor Download PDFInfo
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- CN107841421A CN107841421A CN201711393831.XA CN201711393831A CN107841421A CN 107841421 A CN107841421 A CN 107841421A CN 201711393831 A CN201711393831 A CN 201711393831A CN 107841421 A CN107841421 A CN 107841421A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
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Abstract
The invention discloses a kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, the present invention uses blake bottle of the glass wine bottle as golden Periostracum cicadae, golden Periostracum cicadae is cultivated in glass wine bottle, and the direct filling white wine after culture terminates, it enormously simplify the production process of golden Periostracum cicadae health liquor, reduce the production time of golden Periostracum cicadae health liquor, reduce production cost, and prepared golden Periostracum cicadae health liquor has good health-care effect, there are wide market prospects.
Description
Technical field
The present invention relates to golden cicada flower culture technique field, is specifically that one kind is cultivated golden Periostracum cicadae bacterium and made in vial
The method of standby health liquor.
Background technology
Golden cicada flower is commonly called as big cordyceps sinensis, belongs to entomogenous fungi；In the whole world it has been found that 504 kinds of cordyceps sinensis in, golden cicada flower, the winter
Worm summer grass is only two kinds therein.Domestic and international research shows that golden cicada flower, which has, to be improved immunity, antifatigue, guarantor's kidney, improves and sleep
The multiple actions such as dormancy, antitumor, liver protection, radioresistance and improving eyesight, it is magical ancient Chinese medicine.The golden cicada flower manually cultivated contains sweet dew
Alcohol 2.18%, polysaccharide 21.73%, amino acid/11 9.76%, cordycepin 0.005%, adenosine 0.05%, this is similar to natural cordyceps,
But the toxic heavy metals such as arsenic, mercury, lead do not detect, this is than natural cordyceps safety.Therefore, golden cicada, which is spent, can be used as the worm summer in winter
The substitute of grass, can equally have the function that nourishing and health preserving.
Existing thousands of years history of health liquor, it is the important component of chinese medicine science.The successive dynasties medicine of China writes
Almost there is the record that medicinal liquor controls disease body-building in work bar none.Today with science and technology progress, from the biography of Traditional Chinese medicinal wine
Had developed on the basis of system technique using extraction, extraction and the modernization meanses such as bioengineering, extract in Chinese medicine it is effective into
The functional medicinal-liquor of high content is made in part.
As the improvement of people's living standards, the commercially available fast development of health liquor.If golden Periostracum cicadae system can be used
The standby health liquor with good health-care effect, will be welcome by market.
The content of the invention
It is an object of the invention to provide a kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor,
To solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, step are as follows：
1）Vial as bottle is cleaned, dried, sterilization treatment, obtains pretreated glass bottle, it is standby；
2）Wheat is taken, cleans, after drying, adds into pretreated glass bottle, then adds water into pretreated glass bottle；
3）The inoculation yeast bacterium into pretreated glass bottle, it is sealed by fermentation 7-9 days at 23-26 DEG C, then heats to 35-38 DEG C,
Continue fermentation process 6-7 days；
4）Compound amino-acid nutrition liquid is added into pretreated glass bottle, after addition, by pretreated glass bottle at 50 DEG C
Isothermal holding 1h, room temperature is then naturally cooled to, obtain blake bottle；
5）The golden Cordyceps cicadae strain of laboratory cultures is seeded in blake bottle, carried out under conditions of 20-36 DEG C incubated；
6）In homoisothermal cultivation process, once-combined amino-acid nutrition liquid is added week about, after cultivating four weeks, golden Cordyceps cicadae strain
Growth and maturity simultaneously grow conidia powder, and culture terminates；
7）Blake bottle after terminating to culture carries out sterilization treatment, after sterilization treatment, the filling white wine into blake bottle, i.e.,
Can.
As the further scheme of the present invention：The vial includes bottle, and bottle body bottom is provided with the loudspeaker of downward flaring
The bottom cavity of mouth shape.
As further scheme of the invention：Step 2）In, the volume addition of the wheat is the bottom cavity of vial
The 50-60% of volume.
As further scheme of the invention：Step 2）In, the volume addition of the water is wheat volume addition
1.2-1.5 times.
As further scheme of the invention：Step 3）In, the addition of the saccharomycete is wheat gross weight
0.05-0.06%。
As further scheme of the invention：Step 4）In, the volume addition of the compound amino-acid nutrition liquid is
The 10-15% of wheat volume addition.
As further scheme of the invention：Step 5）In, incubated temperature is 26 DEG C.
As further scheme of the invention：Step 6）In, each volume of the compound amino-acid nutrition liquid adds
Amount is step 2）Described in wheat volume addition 6-10%.
As further scheme of the invention：The compound amino-acid nutrition liquid is food-grade compound amino acid nutrition
Liquid.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention uses blake bottle of the glass wine bottle as golden Periostracum cicadae, and golden Periostracum cicadae is trained in glass wine bottle
Support, and the direct filling white wine after culture terminates, the production process of golden Periostracum cicadae health liquor is enormously simplify, reduces golden cicada
The production time of flower Chinese caterpillar fungus health wine, production cost is reduced, and prepared golden Periostracum cicadae health liquor has good guarantor
Strong effect, has wide market prospects.
Brief description of the drawings
Fig. 1 is the structural representation of the vial used in the present invention.
In figure：1- bottles, 2- bottom cavities.
Embodiment
Technical scheme is described in more detail with reference to embodiment.
Referring to Fig. 1, golden Periostracum cicadae bacterium is cultivated in vial for the present invention and is prepared used in the method for health liquor
Vial structural representation, the vial includes bottle 1, and the bottom of bottle 1 is provided with the bell mouth shape of downward flaring
Bottom cavity 2, wheat is added into bottom cavity 2 and fermented, the wheat volumetric expansion after fermentation, mutually extruding, because bottom cavity 2 is in
The bell mouth shape of downward flaring, therefore the active force mutually extruded between wheat will be such that it is filled in bottom cavity 2 and can not be the bottom of from
Deviate from chamber 2, in follow-up filling white wine, to avoid wheat from entering in white wine, and avoid wheat from dropping out when drinking, influence
The quality of the health liquor.
Embodiment 1
A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, step are as follows：
1）Vial as bottle is cleaned, dried, sterilization treatment, obtains pretreated glass bottle, it is standby；
2）Wheat is taken, cleans, after drying, adds into pretreated glass bottle, then adds water into pretreated glass bottle, wherein, institute
The volume addition for stating wheat is the 50% of the volume of bottom cavity 2 of vial, and the volume addition of the water is wheat volume addition
1.2 times；
3）The inoculation yeast bacterium into pretreated glass bottle, it is sealed by fermentation 7 days at 23 DEG C, then heats to 35 DEG C, continue to ferment
Processing 6 days, wherein, the addition of the saccharomycete is the 0.05% of wheat gross weight；
4）Compound amino-acid nutrition liquid is added into pretreated glass bottle, after addition, by pretreated glass bottle at 50 DEG C
Isothermal holding 1h, room temperature is then naturally cooled to, obtain blake bottle, wherein, the volume of the compound amino-acid nutrition liquid adds
Measure as the 10% of wheat volume addition；
5）The golden Cordyceps cicadae strain of laboratory cultures is seeded in blake bottle, carried out under conditions of 20 DEG C incubated；
6）In homoisothermal cultivation process, once-combined amino-acid nutrition liquid is added week about, after cultivating four weeks, golden Cordyceps cicadae strain
Growth and maturity simultaneously grow conidia powder, and culture terminates, wherein, each volume addition of the compound amino-acid nutrition liquid is step
Rapid 2）Described in wheat volume addition 6%；
7）Blake bottle after terminating to culture carries out sterilization treatment, after sterilization treatment, the filling white wine into blake bottle, i.e.,
Can.
The compound amino-acid nutrition liquid is food-grade compound amino-acid nutrition liquid.
Embodiment 2
A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, step are as follows：
1）Vial as bottle is cleaned, dried, sterilization treatment, obtains pretreated glass bottle, it is standby；
2）Wheat is taken, cleans, after drying, adds into pretreated glass bottle, then adds water into pretreated glass bottle, wherein, institute
The volume addition for stating wheat is the 55% of the volume of bottom cavity 2 of vial, and the volume addition of the water is wheat volume addition
1.3 times；
3）The inoculation yeast bacterium into pretreated glass bottle, it is sealed by fermentation 7 days at 24 DEG C, then heats to 36 DEG C, continue to ferment
Processing 6 days, wherein, the addition of the saccharomycete is the 0.05% of wheat gross weight；
4）Compound amino-acid nutrition liquid is added into pretreated glass bottle, after addition, by pretreated glass bottle at 50 DEG C
Isothermal holding 1h, room temperature is then naturally cooled to, obtain blake bottle, wherein, the volume of the compound amino-acid nutrition liquid adds
Measure as the 11% of wheat volume addition；
5）The golden Cordyceps cicadae strain of laboratory cultures is seeded in blake bottle, carried out under conditions of 25 DEG C incubated；
6）In homoisothermal cultivation process, once-combined amino-acid nutrition liquid is added week about, after cultivating four weeks, golden Cordyceps cicadae strain
Growth and maturity simultaneously grow conidia powder, and culture terminates, wherein, each volume addition of the compound amino-acid nutrition liquid is step
Rapid 2）Described in wheat volume addition 7%；
7）Blake bottle after terminating to culture carries out sterilization treatment, after sterilization treatment, the filling white wine into blake bottle, i.e.,
Can.
The compound amino-acid nutrition liquid is food-grade compound amino-acid nutrition liquid.
Embodiment 3
A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, step are as follows：
1）Vial as bottle is cleaned, dried, sterilization treatment, obtains pretreated glass bottle, it is standby；
2）Wheat is taken, cleans, after drying, adds into pretreated glass bottle, then adds water into pretreated glass bottle, wherein, institute
The volume addition for stating wheat is the 55% of the volume of bottom cavity 2 of vial, and the volume addition of the water is wheat volume addition
1.3 times；
3）The inoculation yeast bacterium into pretreated glass bottle, it is sealed by fermentation 8 days at 25 DEG C, then heats to 36 DEG C, continue to ferment
Processing 7 days, wherein, the addition of the saccharomycete is the 0.06% of wheat gross weight；
4）Compound amino-acid nutrition liquid is added into pretreated glass bottle, after addition, by pretreated glass bottle at 50 DEG C
Isothermal holding 1h, room temperature is then naturally cooled to, obtain blake bottle, wherein, the volume of the compound amino-acid nutrition liquid adds
Measure as the 13% of wheat volume addition；
5）The golden Cordyceps cicadae strain of laboratory cultures is seeded in blake bottle, carried out under conditions of 26 DEG C incubated；
6）In homoisothermal cultivation process, once-combined amino-acid nutrition liquid is added week about, after cultivating four weeks, golden Cordyceps cicadae strain
Growth and maturity simultaneously grow conidia powder, and culture terminates, wherein, each volume addition of the compound amino-acid nutrition liquid is step
Rapid 2）Described in wheat volume addition 8%；
7）Blake bottle after terminating to culture carries out sterilization treatment, after sterilization treatment, the filling white wine into blake bottle, i.e.,
Can.
The compound amino-acid nutrition liquid is food-grade compound amino-acid nutrition liquid.
Embodiment 4
A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, step are as follows：
1）Vial as bottle is cleaned, dried, sterilization treatment, obtains pretreated glass bottle, it is standby；
2）Wheat is taken, cleans, after drying, adds into pretreated glass bottle, then adds water into pretreated glass bottle, wherein, institute
The volume addition for stating wheat is the 58% of the volume of bottom cavity 2 of vial, and the volume addition of the water is wheat volume addition
1.4 times；
3）The inoculation yeast bacterium into pretreated glass bottle, it is sealed by fermentation 9 days at 25 DEG C, then heats to 38 DEG C, continue to ferment
Processing 6 days, wherein, the addition of the saccharomycete is the 0.05% of wheat gross weight；
4）Compound amino-acid nutrition liquid is added into pretreated glass bottle, after addition, by pretreated glass bottle at 50 DEG C
Isothermal holding 1h, room temperature is then naturally cooled to, obtain blake bottle, wherein, the volume of the compound amino-acid nutrition liquid adds
Measure as the 14% of wheat volume addition；
5）The golden Cordyceps cicadae strain of laboratory cultures is seeded in blake bottle, carried out under conditions of 30 DEG C incubated；
6）In homoisothermal cultivation process, once-combined amino-acid nutrition liquid is added week about, after cultivating four weeks, golden Cordyceps cicadae strain
Growth and maturity simultaneously grow conidia powder, and culture terminates, wherein, each volume addition of the compound amino-acid nutrition liquid is step
Rapid 2）Described in wheat volume addition 8%；
7）Blake bottle after terminating to culture carries out sterilization treatment, after sterilization treatment, the filling white wine into blake bottle, i.e.,
Can.
The compound amino-acid nutrition liquid is food-grade compound amino-acid nutrition liquid.
Embodiment 5
A kind of method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, step are as follows：
1）Vial as bottle is cleaned, dried, sterilization treatment, obtains pretreated glass bottle, it is standby；
2）Wheat is taken, cleans, after drying, adds into pretreated glass bottle, then adds water into pretreated glass bottle, wherein, institute
The volume addition for stating wheat is the 60% of the volume of bottom cavity 2 of vial, and the volume addition of the water is wheat volume addition
1.5 times；
3）The inoculation yeast bacterium into pretreated glass bottle, it is sealed by fermentation 9 days at 26 DEG C, then heats to 38 DEG C, continue to ferment
Processing 7 days, wherein, the addition of the saccharomycete is the 0.06% of wheat gross weight；
4）Compound amino-acid nutrition liquid is added into pretreated glass bottle, after addition, by pretreated glass bottle at 50 DEG C
Isothermal holding 1h, room temperature is then naturally cooled to, obtain blake bottle, wherein, the volume of the compound amino-acid nutrition liquid adds
Measure as the 15% of wheat volume addition；
5）The golden Cordyceps cicadae strain of laboratory cultures is seeded in blake bottle, carried out under conditions of 36 DEG C incubated；
6）In homoisothermal cultivation process, once-combined amino-acid nutrition liquid is added week about, after cultivating four weeks, golden Cordyceps cicadae strain
Growth and maturity simultaneously grow conidia powder, and culture terminates, wherein, each volume addition of the compound amino-acid nutrition liquid is step
Rapid 2）Described in wheat volume addition 10%；
7）Blake bottle after terminating to culture carries out sterilization treatment, after sterilization treatment, the filling white wine into blake bottle, i.e.,
Can.
The compound amino-acid nutrition liquid is food-grade compound amino-acid nutrition liquid.
The present invention uses blake bottle of the glass wine bottle as golden Periostracum cicadae, and golden Periostracum cicadae is carried out in glass wine bottle
Culture, and the direct filling white wine after culture terminates, enormously simplify the production process of golden Periostracum cicadae health liquor, reduce gold
The production time of Periostracum cicadae health liquor, production cost is reduced, and prepared golden Periostracum cicadae health liquor has well
Health-care effect, there are wide market prospects.
The better embodiment of the present invention is explained in detail above, but the present invention is not limited to above-mentioned embodiment party
Formula, can also be on the premise of present inventive concept not be departed from one skilled in the relevant art's possessed knowledge
Various changes can be made.
Claims (9)
- A kind of 1. method that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, it is characterised in that step is as follows：1）Vial as bottle is cleaned, dried, sterilization treatment, obtains pretreated glass bottle, it is standby；2）Wheat is taken, cleans, after drying, adds into pretreated glass bottle, then adds water into pretreated glass bottle；3）The inoculation yeast bacterium into pretreated glass bottle, it is sealed by fermentation 7-9 days at 23-26 DEG C, then heats to 35-38 DEG C, Continue fermentation process 6-7 days；4）Compound amino-acid nutrition liquid is added into pretreated glass bottle, after addition, by pretreated glass bottle at 50 DEG C Isothermal holding 1h, room temperature is then naturally cooled to, obtain blake bottle；5）The golden Cordyceps cicadae strain of laboratory cultures is seeded in blake bottle, carried out under conditions of 20-36 DEG C incubated；6）In homoisothermal cultivation process, once-combined amino-acid nutrition liquid is added week about, after cultivating four weeks, golden Cordyceps cicadae strain Growth and maturity simultaneously grow conidia powder, and culture terminates；7）Blake bottle after terminating to culture carries out sterilization treatment, after sterilization treatment, the filling white wine into blake bottle, i.e., Can.
- 2. the method according to claim 1 that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, its feature It is, step 1）In, the vial includes bottle（1）, bottle（1）Bottom is provided with the bottom cavity of the bell mouth shape of downward flaring （2）.
- 3. the method according to claim 2 that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, its feature It is, step 2）In, the volume addition of the wheat is the bottom cavity of vial（2）The 50-60% of volume.
- 4. the method according to claim 3 that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, its feature It is, step 2）In, the volume addition of the water is 1.2-1.5 times of wheat volume addition.
- 5. according to any described methods that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor of claim 1-4, Characterized in that, step 3）In, the addition of the saccharomycete is the 0.05-0.06% of wheat gross weight.
- 6. the method according to claim 5 that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, its feature It is, step 4）In, the volume addition of the compound amino-acid nutrition liquid is the 10-15% of wheat volume addition.
- 7. the method according to claim 5 that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, its feature It is, step 5）In, incubated temperature is 26 DEG C.
- 8. the method according to claim 5 that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, its feature It is, step 6）In, each volume addition of the compound amino-acid nutrition liquid is step 2）Described in wheat volume The 6-10% of addition.
- 9. the method according to claim 8 that golden Periostracum cicadae bacterium is cultivated in vial and prepares health liquor, its feature It is, the compound amino-acid nutrition liquid is food-grade compound amino-acid nutrition liquid.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101168713A (en) * | 2007-10-30 | 2008-04-30 | 张笑容 | Cordyceps sinensis wine and preparation method thereof |
| CN103351972A (en) * | 2013-07-09 | 2013-10-16 | 兴安县锦盛食用菌种植专业合作社 | Method for preparing cordyceps militaris wine |
| CN103598014A (en) * | 2013-11-25 | 2014-02-26 | 湖州新驰医药科技有限公司 | Production method of artificial isaria cicadae miq |
| CN106544242A (en) * | 2015-09-21 | 2017-03-29 | 浙江泛亚生物医药股份有限公司 | A kind of Periostracum cicadae method for preparing medicated wine attractive in appearance |
-
2017
- 2017-12-21 CN CN201711393831.XA patent/CN107841421A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101168713A (en) * | 2007-10-30 | 2008-04-30 | 张笑容 | Cordyceps sinensis wine and preparation method thereof |
| CN103351972A (en) * | 2013-07-09 | 2013-10-16 | 兴安县锦盛食用菌种植专业合作社 | Method for preparing cordyceps militaris wine |
| CN103598014A (en) * | 2013-11-25 | 2014-02-26 | 湖州新驰医药科技有限公司 | Production method of artificial isaria cicadae miq |
| CN106544242A (en) * | 2015-09-21 | 2017-03-29 | 浙江泛亚生物医药股份有限公司 | A kind of Periostracum cicadae method for preparing medicated wine attractive in appearance |
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