CN108264390A - A kind of industrial planting method and its culture medium of Phellinus fructification - Google Patents
A kind of industrial planting method and its culture medium of Phellinus fructification Download PDFInfo
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- CN108264390A CN108264390A CN201810250949.5A CN201810250949A CN108264390A CN 108264390 A CN108264390 A CN 108264390A CN 201810250949 A CN201810250949 A CN 201810250949A CN 108264390 A CN108264390 A CN 108264390A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The invention discloses a kind of industrial planting methods and its culture medium of Phellinus fructification; it includes Phellinus seed and prepares, culture medium preparation, inoculation, training orientation, harvesting, drying steps are factory produced, and wherein solid spawn expansion culture medium includes corn flour, maltose, peptone, MgSO4, KH2P04, agar；Liquid spawn expands culture medium and includes glucose, dusty yeast, MgSO4, KH2PO4, Triammonium citrate, ramulus mori water extract, vitamin B1；Culture medium is factory produced and includes foodstuff seed, distilled water, MgSO4, KH2P04, ramulus mori water extract, pollution rate of the present invention is low, and cultivation cycle is short, product quality is stable, at low cost, can realize batch production, standardized production.Culture medium make the phellinus liteus speed of growth it is fast, it is vigorous, obtain the Phellinus grain weight big time it is short; raw material are simply easily purchased, cheap, shorten Phellinus fructification planting time; biological transformation ratio is high, and polysaccharide therein, flavones, three note component contents can be kept higher.
Description
Technical field：
The present invention relates to a kind of industrial planting methods and its culture medium of Phellinus fructification, belong to edible and medical fungi cultivation technique neck
Domain is related to the cultural method of rare Medicinal Fungus Phellinus igniarius, using the method, it can be achieved that the batch production of Phellinus, standardization, sterilization
Cultivation.
Background technology：
Phellinus（Phellinus igniarius）Also known as Phellinus wild rice, phellinus, Phellinus mushroom, Japanese apricot bacterium, it is subordinate to Basidiomycotina
（Basidiomycotina）, Hymenomycetes（Hymenomycetes）, Polyporaceae（Polyporaceae）, wood layer hole strain
（Puccinia pusilla）, it is a kind of rare medicinal fungus being born on the broad leaf trees trunk such as poplar, willow, is longer than China North China, west
The areas such as north, Heilungkiang, Jilin, Taiwan, Guangdong, Sichuan, Yunnan, Tibet, distribution is wider, is a kind of Chinese medicine of preciousness.
Due to the complexity and particularity of Phellinus physiological ecological and the uncertainty of external environment condition, Phellinus is formed in nature
Fructification needs many years, and due to excessively picking, causes wild Phellinus very rare, price is also once up to per kilogram
5000 ~ 8000 yuans.
Phellinus contains a variety of active ingredients such as abundant flavonoids, polysaccharide, triterpenes, has anti-oxidant, antitumor, anti-
The effect of effect of inflammation, strengthen immunity and repair cell function has significant hemostasis, and promoting blood circulation is changed and drunk, antidiarrheal etc..Phellinus
No toxicity is confirmed to be simultaneously, nursing experiment is carried out to mouse, phenomenon without exception generates, and has to Mouse Liver alcoholism
There is detoxifying effect.
Phellinus artificial cultivation has been carried out at present, has a cultivation in South Korea, China, Japan, method be using linden or
Bacterium bag is cultivated, and fructification harvest needs 3 years or more, since cultivation period is long, environmental exposure, leads to pollution rate height, product quality
Inhomogenous, finished product price height.Yield and quality and price composite factor, limit the further development and application of Phellinus.
The cultivation period of particularly existing Phellinus fructification is too long, can not realize batch production, mechanized farming, Phellinus factory
Change cultivation and still belong to blank.How to develop cultivation cycle is short, can standardize, batch production, mechanization production, effectively solve culture
The pollution problem of process, and product quality is kept to stablize, the Phellinus cultivation technique that production cost is greatly lowered is made to become urgently
The critical issue of solution.
Invention content：
A kind of factory culture side of Phellinus fructification is provided it is an object of the invention to overcome the shortcomings of above-mentioned prior art
Method and its culture medium, for the cultural method whole process in culture in glassware, pollution rate is low, cultivation cycle is short, product quality is stable, into
This is low, can realize batch production, standardized production.The solid spawn expansion culture medium phellinus liteus speed of growth used is most fast, bacterium
Filament color is deeper, it is not easy to be degenerated to white, the most vigorous densification of mycelium；Liquid spawn used expands culture medium, can be with
A large amount of Phellinus seed is obtained in a short time；Factorial praluction culture medium raw material used are simply easily purchased, cheap, are made
Phellinus fructification planting time shortens, and biological transformation ratio is high, and polysaccharide therein, flavones, three note component contents can be kept higher.
The purpose of the present invention can be reached by following measure：
A kind of solid spawn of factory culture for Phellinus fructification expands culture medium, it is characterised in that culture medium raw material packet
It includes：10-50g/L corn flour, 10-50g/L maltose, 0.5-5g/L peptones, O.1-2.0g/L MgSO4,1-6g/L
KH2P04,15-25g/L agar, excess water, pH are natural；This culture medium is by screening the most suitable of the Phellinus found growth
Solid spawn expands culture medium, and on this culture medium, the phellinus liteus speed of growth is most fast, and mycelium color is deeper, it is not easy to move back
Chemical conversion white, the most vigorous densification of mycelium will be that Phellinus cultivation lays the foundation into fructification.
A kind of liquid spawn of factory culture for Phellinus fructification expands culture medium, it is characterised in that culture base
Material includes：30-50g/L glucose, 5-15g/L dusty yeasts, 1-5g/L MgSO4,1-5g/L KH2PO4,1-5g/L citric acids three
Ammonium, 1-5g/L ramulus mori water extracts, O.05-3g/L vitamin B1, excess water, pH are natural；This culture medium is found by screening
The most suitable liquid spawn of Phellinus growth expands culture medium, can obtain a large amount of Phellinus seed in a short time, to reduce
Production cycle, large-scale industrial production Phellinus fructification lay the foundation.
A kind of factorial praluction culture medium of factory culture for Phellinus fructification, it is characterised in that culture medium raw material
Including：Light culture base weight amount percentages, it contains：40-50% is without the foodstuff seed that goes mouldy, 40-50% distilled water, 0.1-3.0%
MgSO4,0.1-3.0% KH2P04,1-10% ramulus mori water extract, pH is naturally, the foodstuff seed is rice, millet, height
One kind in fine strain of millet, wheat, oat, corn；The factorial praluction culture medium raw material are simply easily purchased, cheap, are cultivated herein
Phellinus fructification can be made to be shortened to 6 months by the traditional cultivation time on base, while biological transformation ratio is high, can keep therein
Polysaccharide, flavones, three note component contents are higher.
A kind of industrial planting method of Phellinus fructification, it is characterised in that it is comprised the following specific steps that：
（1）Strain：Phellinus strain is stored on PDA slant mediums；
（2）It is prepared by seed：Under conditions of the growth of suitable Phellinus, phellinus liteus to logarithm is cultivated in liquid seed culture medium and is given birth to
For a long time, liquid Phellinus seed is obtained；Or phellinus liteus is cultivated in solid seed culture medium, obtain solid Phellinus seed；Its
It is as follows：
Preserve the activation of strain：The solid seed culture medium of actication of culture uses carrot Solid agar culture, is inoculated with Phellinus
28 DEG C of temperature after slant strains, light culture 8-12 days；
The expansion culture of solid spawn：The ingredient that solid spawn expansion culture medium uses is 10-20g/L corn flour, 10-20g/L
Maltose, 0.5-1g/L peptones, O.1-0.6g/L MgSO4,1-4g/L KH2P04,15-25g/L agar, excess water, pH
It is natural；After the Phellinus strain of activation is inoculated with, 25 DEG C of cultivation temperature is finally obtained for workshop sufficient amount and high-quality quality
Solid Phellinus seed；
The expansion culture of liquid spawn：The ingredient that liquid spawn expansion culture medium uses is 30-50g/L glucose, 5-15g/L ferment
O.05-3g/L, female powder, 1-5g/L MgSO4,1-5g/L KH2PO4,1-5g/L Triammonium citrates, 1-5g/L ramulus mori water extracts are tieed up
Raw element B1, excess water, PH are natural；After the Phellinus strain of activation is inoculated with, 20-30 DEG C of cultivation temperature, rotating speed 120-130rpm,
Finally obtain the liquid Phellinus seed for the sufficient amount of high-quality quality of workshop；
（3）Culture medium is factory produced to prepare：Light culture base weight amount percentages, it contains：40-50% is without grain kind of going mouldy
Son, 40-50% distilled water, 0.1-3.0% MgSO4,0.1-3.0% KH2P04,1-10% ramulus mori water extracts will be cultivated above
Based component is uniformly mixed according to the ratio, after sealing, at 121 DEG C, and 103.4kPa sterilizing 1h, the foodstuff seed
For one kind in rice, millet, sorghum, wheat, oat, corn；
（4）Inoculation, training orientation, harvesting：In an aseptic environment, liquid Phellinus seed obtained or solid Phellinus seed are inoculated with
Onto sterilized factorial praluction culture medium, light culture 6 months in 24-28 DEG C of culturing room are placed in, harvest Phellinus fructification；Secretly
Culture medium humidity 62%-68% in incubation, air humidity 60%-70%, culturing room will be through hold-open door windowing ventilations, to protect
Demonstrate,prove sufficient oxygen supply amount, it is desirable that indoor unglazed；
（5）It is dry：The Phellinus fructification of harvesting dry 12h in 50 DEG C of -60 DEG C of thermostatic drying chambers, makes Phellinus fructification water content
Reach less than 1.3%.
The present invention can generate following good effect compared with the prior art：The present invention is to utilize grain matrix medium culture
Phellinus bacterium can obtain Phellinus fructification in a short time, and it is few to well solve precious medicinal fungi, experiment material source
The problem of few.The Phellinus fructification active constituent obtained simultaneously is high, can be used for the exploitation of health products, generates certain economic effect
Benefit；For entire cultivation in culture in glassware, pollution rate is low, and cultivation cycle is short, product quality is stable, at low cost, can realize factory
Change, standardized production.
Description of the drawings：
Fig. 1 is that bottle plants Phellinus fructification situation map on the different grain culture mediums of the present invention.
Specific embodiment：It elaborates below to the specific embodiment of the present invention：
Embodiment 1：Rice is primary raw material.
（1）Strain：Phellinus strain is purchased from Guangdong Culture Collection, is stored on PDA slant mediums.
（2）It is prepared by seed：Under conditions of the growth of suitable Phellinus, phellinus liteus is cultivated in liquid seed culture medium to right
Number growth period obtains liquid Phellinus seed；Or phellinus liteus is cultivated in solid seed culture medium, obtain solid Phellinus kind
Son is as follows：
Preserve the activation of strain：The solid seed culture medium of actication of culture uses carrot Solid agar culture, is inoculated with inclined-plane
28 DEG C of temperature after strain, light culture 8 days.
The expansion culture of solid spawn：The ingredient that solid spawn expansion culture medium uses is 20g/L corn flour, 20g/L wheats
Bud sugar, 1g/L peptones, 0.6g/L MgSO4,1g/L KH2P04,15g/L agar, excess water, pH are natural；By the Phellinus of activation
After strain inoculation, 25 DEG C of cultivation temperature finally obtains the solid Phellinus seed for workshop sufficient amount and high-quality quality.
The expansion culture of liquid spawn：The ingredient that liquid spawn expansion culture medium uses is 30g/L glucose, 5g/L yeast
O.05g/L, powder, 1g/L MgSO4,1g/L KH2PO4,2g/L Triammonium citrates, 1g/L ramulus mori water extracts give birth to element B1, surplus
Water, PH are natural.After the Phellinus strain of activation is inoculated with, 20 DEG C, rotating speed 120rpm of cultivation temperature is finally obtained for workshop foot
The liquid Phellinus seed of the high-quality quality of enough amounts.
（3）Culture medium is factory produced to prepare：Based on culture medium weight percent, it contains：50% nothing is gone mouldy rice kind
Son, 45% distilled water, 2.0% MgSO4,2.0% KH2P04,1% ramulus mori water extract, by more than medium component according to described
Ratio is uniformly mixed, after sealing, at 121 DEG C, and 103.4kPa sterilizings 1h.
（4）Inoculation, training orientation, harvesting：In an aseptic environment, by liquid Phellinus seed obtained or solid Phellinus seed
It is inoculated on sterilized factorial praluction culture medium, is placed in light culture 6 months in 26 DEG C of culturing room, harvests Phellinus fructification；
Culture medium humidity 62% during light culture, air humidity 68%, culturing room will be through hold-open door windowing ventilations, to ensure abundance
Oxygen supply amount, it is desirable that it is indoor unglazed；
（5）It is dry：The Phellinus fructification of harvesting dry 12h in 60 DEG C of thermostatic drying chambers, reaches Phellinus fructification water content
Less than 1.3%.
Sample result：Weight in wet base：180.90g/ jin（Culture medium）；Dry weight：52g/ jins（Culture medium）.
Using rice as culture raw material culture Phellinus, active constituent therein is extracted using certain method, content is：Polysaccharide
Content：19.49mg/g ；Flavones content：11.82mg/g ；Triterpene content：0.43mg/g.
Embodiment 2：With millet primary raw material
（1）Strain：Phellinus strain is purchased from Guangdong Culture Collection, is stored on PDA slant mediums.
（2）It is prepared by seed：Under conditions of the growth of suitable Phellinus, phellinus liteus is cultivated in liquid seed culture medium to right
Number growth period obtains liquid Phellinus seed；Or phellinus liteus is cultivated in solid seed culture medium, obtain solid Phellinus kind
Son is as follows：
Preserve the activation of strain：The solid seed culture medium of actication of culture uses carrot Solid agar culture, is inoculated with inclined-plane
28 DEG C of temperature after strain, light culture 12 days.
The expansion culture of solid spawn：The ingredient that solid spawn expansion culture medium uses is 15g/L corn flour, 15g/L wheats
Bud sugar, 0.8g/L peptones, 0.5g/L MgSO4,3g/L KH2P04,20g/L agar, excess water, pH are natural；By activation
After the inoculation of Phellinus strain, 25 DEG C of cultivation temperature finally obtains the solid Phellinus kind for workshop sufficient amount and high-quality quality
Son.
The expansion culture of liquid spawn：The ingredient that liquid spawn expansion culture medium uses is 40g/L glucose, 10g/L ferment
Female powder, 3g/L MgSO4,2g/L KH2PO4,1g/L Triammonium citrates, 2g/L ramulus mori water extracts, 0.1g/L vitamin B1s are remaining
Water is measured, PH is natural.After the Phellinus strain of activation is inoculated with, 25 DEG C, rotating speed 125rpm of cultivation temperature is finally obtained for workshop
The liquid Phellinus seed of sufficient amount of high-quality quality.
（3）Culture medium is factory produced to prepare：Based on culture medium weight percent, it contains：45% nothing is gone mouldy millet kind
Son, 40% distilled water, 2.5% MgSO4,2.5% KH2P04,10% ramulus mori water extract, by more than medium component according to described
Ratio is uniformly mixed, after sealing, at 121 DEG C, and 103.4kPa sterilizings 1h；
（4）Inoculation, training orientation, harvesting：In an aseptic environment, liquid Phellinus seed obtained or solid Phellinus seed are inoculated with
Onto sterilized factorial praluction culture medium, light culture 6 months in 24 DEG C of culturing room are placed in, harvest Phellinus fructification；Dark training
Culture medium humidity 65% during supporting, air humidity 60%, culturing room will be through hold-open door windowing ventilations, to ensure sufficient oxygen
Gas supply, it is desirable that indoor unglazed；
（5）It is dry：The Phellinus fructification of harvesting dry 12h in 50 DEG C of thermostatic drying chambers, reaches Phellinus fructification water content
Less than 1.3%.
Sample result：Weight in wet base：103.4g/ jin（Culture medium）；Dry weight：27.1g/ jins（Culture medium）.
Using millet as culture raw material culture Phellinus, active constituent therein is extracted using certain method, content is：Polysaccharide
Content：10.77mg/g ；Flavones content：3.9mg/g ；Triterpene content：0.7mg/g.
Embodiment 3：With oat primary raw material
（1）Strain：Phellinus strain is purchased from Guangdong Culture Collection, is stored on PDA slant mediums.
（2）It is prepared by seed：Under conditions of the growth of suitable Phellinus, phellinus liteus is cultivated in liquid seed culture medium to right
Number growth period obtains liquid Phellinus seed；Or phellinus liteus is cultivated in solid seed culture medium, obtain solid Phellinus kind
Son is as follows：
Preserve the activation of strain：The solid seed culture medium of actication of culture uses carrot Solid agar culture, is inoculated with inclined-plane
28 DEG C of temperature after strain, light culture 10 days.
The expansion culture of solid spawn：The ingredient that solid spawn expansion culture medium uses is 10g/L corn flour, 10g/L wheats
Bud sugar, 0.5g/L peptones, 0.1g/L MgSO4,4g/L KH2P04,25g/L agar, excess water, pH are natural；By the mulberry of activation
After yellow strain inoculation, 25 DEG C of cultivation temperature finally obtains the solid Phellinus seed for workshop sufficient amount and high-quality quality.
The expansion culture of liquid spawn：The ingredient that liquid spawn expansion culture medium uses is 50g/L glucose, 15g/L ferment
Female powder, 5g/L MgSO4,5g/L KH2PO4,5g/L Triammonium citrates, 5g/L ramulus mori water extracts, 3g/L life element B1, excess water,
PH is natural.After the Phellinus strain of activation is inoculated with, 30 DEG C, rotating speed 130rpm of cultivation temperature finally obtains enough for workshop
The liquid Phellinus seed of the high-quality quality of quantity.
（3）Culture medium is factory produced to prepare：Based on culture medium weight percent, it contains：40% nothing is gone mouldy oat kind
Son, 50% distilled water, 3.0% MgSO4,3.0% KH2P04,4% ramulus mori water extract, by more than medium component according to described
Ratio is uniformly mixed, after sealing, at 121 DEG C, and 103.4kPa sterilizings 1h.
（4）Inoculation, training orientation, harvesting：In an aseptic environment, by liquid Phellinus seed obtained or solid Phellinus seed
It is inoculated on sterilized factorial praluction culture medium, is placed in light culture 6 months in 28 DEG C of culturing room, harvests Phellinus fructification；
Culture medium humidity 62% during light culture, air humidity 65%, culturing room will be through hold-open door windowing ventilations, to ensure abundance
Oxygen supply amount, it is desirable that it is indoor unglazed；
（5）It is dry：The Phellinus fructification of harvesting dry 12h in 55 DEG C of thermostatic drying chambers, reaches Phellinus fructification water content
Less than 1.3%.
Sample result：Weight in wet base：141.9g/ jin（Culture medium）；Dry weight：33.85g/ jins（Culture medium）.
Using oat as culture raw material culture Phellinus, active constituent therein is extracted using certain method, content is：Polysaccharide
Content：8.85mg/g ；Flavones content：2.29mg/g ；Triterpene content：0.45mg/g.
Embodiment 4：With wheat bran primary raw material
（1）Strain：Phellinus strain is purchased from Guangdong Culture Collection, is stored on PDA slant mediums.
（2）It is prepared by seed：Under conditions of the growth of suitable Phellinus, phellinus liteus is cultivated in liquid seed culture medium to right
Number growth period obtains liquid Phellinus seed；Or phellinus liteus is cultivated in solid seed culture medium, obtain solid Phellinus kind
Son is as follows：
Preserve the activation of strain：The solid seed culture medium of actication of culture uses carrot Solid agar culture, is inoculated with inclined-plane
28 DEG C of temperature after strain, light culture 10 days.
The expansion culture of solid spawn：The ingredient that solid spawn expansion culture medium uses is 20g/L corn flour, 10g/L wheats
Bud sugar, 0.5g/L peptones, 0.6g/L MgSO4,3g/L KH2P04,15g/L agar, excess water, pH are natural；By the mulberry of activation
After yellow strain inoculation, 25 DEG C of cultivation temperature finally obtains the solid Phellinus seed for workshop sufficient amount and high-quality quality.
The expansion culture of liquid spawn：The ingredient that liquid spawn expansion culture medium uses is 40g/L glucose, 15g/L ferment
Female powder, 3g/L MgSO4,3g/L KH2PO4,3g/L Triammonium citrates, 4g/L ramulus mori water extracts, 2g/L life element B1, excess water,
PH is natural.After the Phellinus strain of activation is inoculated with, 25 DEG C, rotating speed 125rpm of cultivation temperature finally obtains enough for workshop
The liquid Phellinus seed of the high-quality quality of quantity.
（3）Culture medium is factory produced to prepare：Based on culture medium weight percent, it contains：45% nothing go mouldy wheat bran,
45% distilled water, 0.1% MgSO4,0.1% KH2P04,9.8% ramulus mori water extract, by more than medium component according to the ratio
Example is uniformly mixed, after sealing, at 121 DEG C, and 103.4kPa sterilizings 1h.
（4）Inoculation, training orientation, harvesting：In an aseptic environment, by liquid Phellinus seed obtained or solid Phellinus seed
It is inoculated on sterilized factorial praluction culture medium, is placed in light culture 6 months in 27 DEG C of culturing room, harvests Phellinus fructification；
Culture medium humidity 68% during light culture, air humidity 70%, culturing room will be through hold-open door windowing ventilations, to ensure abundance
Oxygen supply amount, it is desirable that it is indoor unglazed；
（5）It is dry：The Phellinus fructification of harvesting dry 12h in 60 DEG C of thermostatic drying chambers, reaches Phellinus fructification water content
Less than 1.3%.
Sample result：Weight in wet base：96.5g/ jins（Culture medium）, 22.6g/ jins of dry weight（Culture medium）.
Using wheat bran as culture raw material culture Phellinus, active constituent therein is extracted using certain method, content is：Polysaccharide
Content：3.68mg/g ；Flavones content：0.39mg/g ；Triterpene content：0.47mg/g.
It should be understood that the part that this specification does not elaborate belongs to the prior art.It is above-mentioned to implement for preferable
The description of example is more careful, but cannot be therefore, it is considered that being the limitation to scope of patent protection of the present invention.
Claims (4)
1. a kind of solid spawn of factory culture for Phellinus fructification expands culture medium, it is characterised in that culture medium raw material
Including：10-50g/L corn flour, 10-50g/L maltose, 0.5-5g/L peptones, O.1-2.0g/L MgSO4,1-6g/L
KH2P04,15-25g/L agar, excess water, pH are natural.
2. a kind of liquid spawn of factory culture for Phellinus fructification expands culture medium, it is characterised in that culture medium raw material
Including：30-50g/L glucose, 5-15g/L dusty yeasts, 1-5g/L MgSO4,1-5g/L KH2PO4,1-5g/L citric acids three
Ammonium, 1-5g/L ramulus mori water extracts, O.05-3g/L vitamin B1, excess water, pH are natural.
A kind of 3. factorial praluction culture medium of factory culture for Phellinus fructification, it is characterised in that culture medium raw material packet
It includes：Light culture base weight amount percentages, 40-50% is without the foodstuff seed that goes mouldy, 40-50% distilled water, 0.1-3.0% MgSO4,
0.1-3.0% KH2P04,1-10% ramulus mori water extracts, pH naturally, the foodstuff seed be rice, millet, sorghum, wheat,
One kind in oat, corn.
4. a kind of industrial planting method of Phellinus fructification, it is characterised in that it is comprised the following specific steps that：
（1）Strain：Phellinus strain is stored on PDA slant mediums；
（2）It is prepared by seed：Under conditions of the growth of suitable Phellinus, phellinus liteus to logarithm is cultivated in liquid seed culture medium and is given birth to
For a long time, liquid Phellinus seed is obtained；Or phellinus liteus is cultivated in solid seed culture medium, obtain solid Phellinus seed；Its
It is as follows：
Preserve the activation of strain：The solid seed culture medium of actication of culture uses carrot Solid agar culture, is inoculated with Phellinus
28 DEG C of temperature after slant strains, light culture 8-12 days；
The expansion culture of solid spawn：The ingredient that solid spawn expansion culture medium uses is 10-20g/L corn flour, 10-20g/L
Maltose, 0.5-1g/L peptones, O.1-0.6g/L MgSO4,1-4g/L KH2P04,15-25g/L agar, excess water, pH
It is natural；After the Phellinus strain of activation is inoculated with, 25 DEG C of cultivation temperature is finally obtained for workshop sufficient amount and high-quality quality
Solid Phellinus seed；
The expansion culture of liquid spawn：The ingredient that liquid spawn expansion culture medium uses is 30-50g/L glucose, 5-15g/L ferment
O.05-3g/L, female powder, 1-5g/L MgSO4,1-5g/L KH2PO4,1-5g/L Triammonium citrates, 1-5g/L ramulus mori water extracts are tieed up
Raw element B1, excess water, PH are natural；After the Phellinus strain of activation is inoculated with, 20-30 DEG C of cultivation temperature, rotating speed 120-130rpm,
Finally obtain the liquid Phellinus seed for the sufficient amount of high-quality quality of workshop；
（3）Culture medium is factory produced to prepare：Light culture base weight amount percentages, it contains：40-50% is without grain kind of going mouldy
Son, 40-50% distilled water, 0.1-3.0% MgSO4,0.1-3.0% KH2P04,1-10% ramulus mori water extracts will be cultivated above
Based component is uniformly mixed according to the ratio, after sealing, at 121 DEG C, and 103.4kPa sterilizing 1h, the foodstuff seed
For one kind in rice, millet, sorghum, wheat, oat, corn；
（4）Inoculation, training orientation, harvesting：In an aseptic environment, liquid Phellinus seed obtained or solid Phellinus seed are inoculated with
Onto sterilized factorial praluction culture medium, light culture 6 months in 24-28 DEG C of culturing room are placed in, harvest Phellinus fructification；Secretly
Culture medium humidity 62%-68% in incubation, air humidity 60%-70%, culturing room will be through hold-open door windowing ventilations, to protect
Demonstrate,prove sufficient oxygen supply amount, it is desirable that indoor unglazed；
（5）It is dry：The Phellinus fructification of harvesting dry 12h in 50 DEG C of -60 DEG C of thermostatic drying chambers, makes Phellinus fructification water content
Reach less than 1.3%.
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| CN201810250949.5A CN108264390A (en) | 2018-03-26 | 2018-03-26 | A kind of industrial planting method and its culture medium of Phellinus fructification |
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Cited By (6)
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| CN109168958A (en) * | 2018-10-11 | 2019-01-11 | 安康学院 | A kind of Phellinus cultural method |
| CN109601245A (en) * | 2018-12-21 | 2019-04-12 | 兰顺明 | A kind of culture medium and its implantation methods of mulberry tree soak weed tree sawdust factory culture Phellinus |
| CN110249912A (en) * | 2019-08-02 | 2019-09-20 | 丁利华 | A kind of method that Phellinus industrial bottle is planted |
| CN112574893A (en) * | 2020-12-16 | 2021-03-30 | 广东省微生物研究所（广东省微生物分析检测中心） | Phellinus baumii, and preparation method and application of fermentation product thereof |
| CN113755348A (en) * | 2021-10-27 | 2021-12-07 | 重庆市蚕业科学技术研究院 | Separation and purification method of wild phellinus igniarius strain |
| CN115362878A (en) * | 2022-08-11 | 2022-11-22 | 西南大学 | Method for artificially simulating natural cultivation of edible and medicinal fungi phellinus igniarius by using whole mulberry branches and application of method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168958A (en) * | 2018-10-11 | 2019-01-11 | 安康学院 | A kind of Phellinus cultural method |
| CN109168958B (en) * | 2018-10-11 | 2020-11-24 | 安康学院 | Phellinus igniarius cultivation method |
| CN109601245A (en) * | 2018-12-21 | 2019-04-12 | 兰顺明 | A kind of culture medium and its implantation methods of mulberry tree soak weed tree sawdust factory culture Phellinus |
| CN110249912A (en) * | 2019-08-02 | 2019-09-20 | 丁利华 | A kind of method that Phellinus industrial bottle is planted |
| CN112574893A (en) * | 2020-12-16 | 2021-03-30 | 广东省微生物研究所（广东省微生物分析检测中心） | Phellinus baumii, and preparation method and application of fermentation product thereof |
| CN113755348A (en) * | 2021-10-27 | 2021-12-07 | 重庆市蚕业科学技术研究院 | Separation and purification method of wild phellinus igniarius strain |
| CN115362878A (en) * | 2022-08-11 | 2022-11-22 | 西南大学 | Method for artificially simulating natural cultivation of edible and medicinal fungi phellinus igniarius by using whole mulberry branches and application of method |
| CN115362878B (en) * | 2022-08-11 | 2024-02-02 | 西南大学 | Method for artificially and naturally cultivating edible and medicinal fungus Phellinus linteus by using whole mulberry branches and application thereof |
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