CN106190855A - Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) - Google Patents
Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) Download PDFInfo
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Abstract
Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae), it is to be that primary raw material does culture medium with Thallus Laminariae (Thallus Eckloniae), cordyceps species after medium sterilization, will be accessed, then cultivate to Cordyceps mycelium and cover with culture medium, then the culture medium covering with mycelia is dried, finally pulverize to obtain Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium.By implementing the present invention, by biotechnology, Thallus Laminariae (Thallus Eckloniae) and Cordyceps are formed the coalition Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium of another characteristic, combine Thallus Laminariae (Thallus Eckloniae) and the nutrient of Cordyceps and action and efficacy so that it is nutrition, function and effect can be more prominent simultaneously, and its health care and diseases prevention, effect of curing the disease are more notable.Meanwhile, by implementing the present invention, the process for producing of final products Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium, there is the features such as cultivation period is short, process for producing simple, the production efficiency of product is high and stablizes.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to one and prepare Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium with Thallus Laminariae (Thallus Eckloniae) for culture medium
Body.
Background technology
Thallus Laminariae (Thallus Eckloniae), is a kind of large-scale sea raw Brown algae plant of growth in low temperature seawater, belongs to seaweed plant, Laminariaceae.Spore
Daughter is large-scale, brown, flat belt-like, the longest up to 20M.Leaflet sheet, shank and holdfast, holdfast is rhizoid shape.Blade is
Epidermis, cortex and marrow tissue are formed, and Sporangium is arranged at blade bottom.There is mucilage cavity, slip material can be secreted.Holdfast tree
Shape branch, in order to adhere to marine rock.It is grown on relatively low marine of water temperature.NORTH CHINA is coastal and Zhejiang, ALONG COASTAL FUJIAN are a large amount of
Cultivation, yield ranks first in the world.Rich in raw materials of industry such as Algin and iodine matter, edible and extraction iodine, Algin, mannitol.
Its thallus can be used as medicine.Thallus Laminariae (Thallus Eckloniae) is the vegetable that a kind of nutritive value is the highest, has certain medical value simultaneously.Containing enrich
The mineral elements such as iodine.Thallus Laminariae (Thallus Eckloniae) heat content is low, protein content is medium, mineral is abundant, and research finds, Thallus Laminariae (Thallus Eckloniae) has fall blood
The multiple biological functions such as fat, blood sugar lowering, regulation immunity, anticoagulation, antitumor, Plumbum removing removing toxic substances and antioxidation.
Thallus Laminariae (Thallus Eckloniae) composition is: fresh goods edible portion 100%.Energy content 70kJ, moisture 94.4g in every 100g, protein
1.2g, fat 0.1g, dietary fiber 0.5g, carbohydrate 1.6g, thiamine 0.02mg, riboflavin 0.15mg, nicotinic acid
1.3mg: vitamin E 1.85mg;Potassium 246mg, sodium 8.6mg, calcium 46mg, iodine 0.28mg, magnesium 25mg, ferrum 0.9mg, manganese 0.
07mg, zinc 0.16mg, phosphorus 22mg, selenium 9.54 μ g.Vitamin C does not detects.Still containing algin acid (up to 32%) and laminaran etc..
The pharmacology of Thallus Laminariae (Thallus Eckloniae) is worth as follows:
1. preventing and treating goiter: Thallus Laminariae (Thallus Eckloniae) contains iodine and iodide, has the effect of preventing and treating goiter due to iodine deficiency.
2. blood pressure lowering: laminine and potassium salt, calcium constituent can reduce the human body absorption to cholesterol, reduce blood pressure.
3. blood fat reducing: Thallus Laminariae (Thallus Eckloniae) contains substantial amounts of unsaturated fatty acid and dietary fiber, can remove the gallbladder being attached in blood vessel wall
Sterin, adjusts along the intestines and stomach, promotes the excretion of cholesterol.
5. improve immunity: Thallus Laminariae (Thallus Eckloniae) can improve the humoral immunization of body, promote the cellular immunization of body, regulation immunity
Food also has a lot, and Lentinus Edodes is exactly one of them.
6. blood sugar lowering: contain the fucoidin of 60% in Thallus Laminariae (Thallus Eckloniae), be fabulous dietary fiber, after patients with diabetes mellitus, can prolong
Slow gastric emptying and the food time by small intestinal, so, even if in the case of amount of insulin secretion reduces, blood sugar content is the most not
Can rise, and reach to treat the purpose of diabetes.
7. diuresis, detumescence: often adhering to white lead--mannitol as one layer of bloom on Thallus Laminariae (Thallus Eckloniae), it is a kind of valuable medicinal thing
Matter.Modern scientific research proves, mannitol have reduce blood pressure, diuresis and the effect of detumescence.
8. prevention cardiovascular and cerebrovascular disease: containing substantial amounts of polyunsaturated fatty acid EPA in Thallus Laminariae (Thallus Eckloniae), the viscosity drop of blood can be made
Low, reduce sclerosis of blood vessels.Therefore, the disease in terms of Thallus Laminariae (Thallus Eckloniae) can prevent cardiovascular is often eaten.
9. eliminate cyclomastopathy: in Thallus Laminariae (Thallus Eckloniae), substantial amounts of iodine can be with Stimulation of Pituitary Gland, and maid's gonosome inner estrogen level reduces, extensive
The normal function of multiple ovary, corrects endocrine disturbance, eliminates the hidden danger of cyclomastopathy.
10. hair care;Iodine in Thallus Laminariae (Thallus Eckloniae) is the abundantest, and it is the primary raw material of internal synthetic thyroid element, and the light of hair
Pool is precisely due to internal thyroxine plays a role and formed.
11. replenish the calcium: Thallus Laminariae (Thallus Eckloniae) contains abundant calcium, can prevent and treat human body calcium deficiency.
12. beauty treatments: kelp juice can be with looks improving and the skin nourishing, and the decoction being boiled into Thallus Laminariae (Thallus Eckloniae) is bathed, and skin of can moistening makes skin clear
Refreshing fine and smooth, bright and clean beauty.
13. fat-reducing: Thallus Laminariae (Thallus Eckloniae) contains substantial amounts of dietary fiber, can increase the satiety of overweight people, and Thallus Laminariae (Thallus Eckloniae) fat contains
Measuring the lowest, heat is little, is the food lost weight of overweight people.
14. slow down aging: kelp Rich contains selenium, can effectively remove free radical, and the chief-criminal of free radical human senility just
Chief culprit, so Thallus Laminariae (Thallus Eckloniae) can be with the effect of slow down aging.
15. anti-cancers: the sodium alginate contained by Thallus Laminariae (Thallus Eckloniae) has the strongest binding ability with the tool strontium of carcinogenesis, cadmium, and by it
Excrete；Thallus Laminariae (Thallus Eckloniae) alternative can produce carcinogenic antibacterial in killing or suppressing intestinal, and contained fiber can also promote
Enter the discharge of bile acid and cholesterol；Thallus Laminariae (Thallus Eckloniae) extract has direct repression to various cancerous cell.
Meanwhile, Thallus Laminariae (Thallus Eckloniae) also to have radioprotective, weight reducing, hair caring, slow down aging, brain boosting and supplementing, the intestines and stomach " street cleaner " etc. relevant
Effect.
Summary of the invention
It is an object of the invention to provide and brand-new prepare Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium with Thallus Laminariae (Thallus Eckloniae) for culture medium.Specifically include:
This mycelium is to be that primary raw material does culture medium with Thallus Laminariae (Thallus Eckloniae), by accessing cordyceps species after medium sterilization, then cultivates to worm
Grass mycelia covers with culture medium, then the culture medium covering with mycelia is dried, finally pulverizes to obtain Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium.
Described Cordyceps, is also Cordyceps militaris, is a kind of rich in cordycepin, high protein, amino acid whose edible fungi, is first-class
Excellent tonic product.Current tame Cordyceps militaris mainly has two classes: a class is that artificial breeding forms with the raw bacterium of worm pupa, and second
Class is modal on market, and artificial breeding forms with frumentum, the Semen Tritici aestivi raw bacterium of fermentation.
Cordyceps militaris (L.) Link. grass length 5～8 centimeters, golden yellow, orange colour, belong to Eumycota Cordyceps with Cordyceps of Qinghai Province,
There is similar medicinal and nourishing effects.Thinking the Research on kinds having become as induction Cordyceps sporophore both at home and abroad, Cordyceps militaris is eaten
Can match in excellence or beauty with traditional Cordyceps with medical value, the Cordyceps militaris cordycepin content of artificial culture is higher than tradition Cordyceps
Tens times.
Modern medicine and pharmacology research proves, Cordyceps militaris (L.) Link. contains rich in protein, 18 kinds of aminoacid, 17 kinds of trace units
Element, 12 kinds of vitamin: A, B1, B2, B6, B12, C, D, E equal size is higher than mushrooms, has nourishing the lung and kidney, hemostasis and phlegm, expansion
The effect such as trachea, calmness, anti-each bacterioid, blood pressure lowering.Through Hong Kong, Korea S, Japan, Canada, the U.S. and domestic state more than ten
Research and the detection of the countries in the world such as individual unit prove, Cordyceps militaris is the Ideal Substitute of tradition Cordyceps.
Cordyceps militaris is prepared as tea beverage, tool defying age, resisting fatigue, anticancer, antiinflammatory, antibacterial, protect the heart, protect the liver, lung moistening, benefit
The multiple medical care effects such as kidney and enhancing body, U.S. face black hair.Also general nutritional health food it is different from only to nutrient in a certain respect
Deficiency carry out supplementing and strengthening, but on the whole two-ways regulation function of human body balance, transfer internal body vigor, regulate people
Body metabolism function, improves autoimmune ability, promotes whole internal organs or organ function normalization.
The present invention is achieved through the following technical solutions:
Prepare Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium with Thallus Laminariae (Thallus Eckloniae) for culture medium, technical scheme is: this mycelium is for the most former with Thallus Laminariae (Thallus Eckloniae)
Material does culture medium, will access cordyceps species after medium sterilization, then cultivates and covers with culture medium to Cordyceps mycelium, then incites somebody to action long
The culture medium of full mycelia is dried, and finally pulverizes to obtain Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium.
Specifically include following steps:
1, Thallus Laminariae (Thallus Eckloniae) gathers and processes: gather that drug residue free, color and luster be normal, free from admixture, fresh Thallus Laminariae (Thallus Eckloniae) are raw material, then by
It is the fragment of 0.5-2 centimetre that Thallus Laminariae (Thallus Eckloniae) is crushed into diameter, finally Thallus Laminariae (Thallus Eckloniae) is done to material moisture standby less than 8% by solarization or roars of laughter
With.
2, preparation culture medium: culture medium is made up of the Thallus Laminariae (Thallus Eckloniae) of 88% and the Semen Maydis powder of 12%, first by kelp soaking in 5%
Among G/W, pulling out and drain away the water after persistently soaking 3-5 hour, Semen Maydis powder is also adjusted to humidity with the G/W of 5%
Content is 55-60%, and mix Thallus Laminariae (Thallus Eckloniae) and Semen Maydis powder and stir to obtain mixed material the most again, mixed material subpackage
In culture vessel, charge weight be mixed material thickness in culture vessel be 2-3cm, the most conventionally after sterilizing
Must produce and use culture medium.
3, inoculation: in an aseptic environment cordyceps species is accessed in culture medium, when access Cordyceps fixes strain, 6-to be accessed
The solid spawn of 8 points, when accessing liquid spawn, culture medium weight to be accessed is the liquid spawn of 10-12%.
4, cultivation: the culture medium connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, the wettest
Degree is to cultivate in the environment of 60～65% to mycelia to cover with culture medium.
5, dry: in the environment of temperature is less than 50 degree, the culture medium covering with mycelia is shone to material moisture less than 8%.
6, finished product: the medium powder dried is broken to can cross 120-200 mesh and obtains finished product Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium.
The present invention both had the following advantages:
1, proposed by the invention to prepare its method of Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium with Thallus Laminariae (Thallus Eckloniae) for culture medium novel, production technology letter
Singly, easily implement.
2, by implementing the present invention, by biotechnology, Thallus Laminariae (Thallus Eckloniae) and Cordyceps are formed the coalition sea of another characteristic
Band Cordyceps mycelium, combines Thallus Laminariae (Thallus Eckloniae) and the nutrient of Cordyceps and action and efficacy so that it is nutrition, function and effect can be more prominent simultaneously
Go out, and its health care and diseases prevention, effect of curing the disease are more notable.
3, by implementing the present invention, the process for producing of final products Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium, there is cultivation week
The features such as the phase is short, process for producing is simple, the production efficiency of product high and stable.
4, by implementing the present invention, the Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium of formation is of many uses, can be as being prepared as medicine, health care
Product, the raw material of Speciality Foods additive.Also can be sub-packed in capsule and become a kind of characteristic and health care food medicine.There not only have to be the best
Market prospect, and the economic benefit produced also can highly significant.
Detailed description of the invention
Below in conjunction with embodiment, the method for the present invention is further illustrated.
Prepare Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium with Thallus Laminariae (Thallus Eckloniae) for culture medium, technical scheme is: this mycelium is to be main with Thallus Laminariae (Thallus Eckloniae)
Want raw material to do culture medium, cordyceps species will be accessed after medium sterilization, and then cultivate to Cordyceps mycelium and cover with culture medium, then
The culture medium covering with mycelia is dried, finally pulverizes to obtain Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium.
Detailed description of the invention is as follows:
1, Thallus Laminariae (Thallus Eckloniae) gathers and processes: gather that drug residue free, color and luster be normal, free from admixture, fresh Thallus Laminariae (Thallus Eckloniae) are raw material, then by
It is the fragment of 0.5-2 centimetre that Thallus Laminariae (Thallus Eckloniae) is crushed into diameter, finally Thallus Laminariae (Thallus Eckloniae) is done to material moisture standby less than 8% by solarization or roars of laughter
With.
2, preparation culture medium: culture medium is made up of the Thallus Laminariae (Thallus Eckloniae) of 88% and the Semen Maydis powder of 12%, first by kelp soaking in 5%
Among G/W, pulling out and drain away the water after persistently soaking 3-5 hour, Semen Maydis powder is also adjusted to humidity with the G/W of 5%
Content is 55-60%, and mix Thallus Laminariae (Thallus Eckloniae) and Semen Maydis powder and stir to obtain mixed material the most again, mixed material subpackage
In culture vessel, charge weight be mixed material thickness in culture vessel be 2-3cm, the most conventionally after sterilizing
Must produce and use culture medium.
3, inoculation: in an aseptic environment cordyceps species is accessed in culture medium, when access Cordyceps fixes strain, 6-to be accessed
The solid spawn of 8 points, when accessing liquid spawn, culture medium weight to be accessed is the liquid spawn of 10-12%.
4, cultivation: the culture medium connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, the wettest
Degree is to cultivate in the environment of 60～65% to mycelia to cover with culture medium.
5, dry: in the environment of temperature is less than 50 degree, the culture medium covering with mycelia is shone to material moisture less than 8%.
6, finished product: the medium powder dried is broken to can cross 120-200 mesh and obtains finished product Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium.
Claims (1)
1. prepare Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium with Thallus Laminariae (Thallus Eckloniae) for culture medium, it is characterised in that: this mycelium is to be primary raw material with Thallus Laminariae (Thallus Eckloniae)
Do culture medium, by accessing cordyceps species after medium sterilization, then cultivate and cover with culture medium to Cordyceps mycelium, then will cover with
The culture medium of mycelia is dried, and finally pulverizes to obtain Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium；
Specifically include following steps:
(1) Thallus Laminariae (Thallus Eckloniae) gathers and processes: gather that drug residue free, color and luster be normal, free from admixture, fresh Thallus Laminariae (Thallus Eckloniae) are raw material, then
It is the fragment of 0.5-2 centimetre that Thallus Laminariae (Thallus Eckloniae) is crushed into diameter, finally Thallus Laminariae (Thallus Eckloniae) is done to material moisture less than 8% by shining or fooling
Standby；
(2) culture medium is prepared: culture medium is made up of the Thallus Laminariae (Thallus Eckloniae) of 88% and the Semen Maydis powder of 12%, first by kelp soaking in the Fructus Vitis viniferae of 5%
Among sucrose solution, pulling out and drain away the water after persistently soaking 3-5 hour, Semen Maydis powder is also adjusted to moisture content with the G/W of 5%
For 55-60%, mix Thallus Laminariae (Thallus Eckloniae) and Semen Maydis powder and stir to obtain mixed material the most again, and mixed material is sub-packed in training
Support in container, charge weight be mixed material thickness in culture vessel be 2-3cm, the most conventionally must be raw after sterilizing
Product culture medium；
(3) inoculation: in an aseptic environment cordyceps species is accessed in culture medium, when access Cordyceps fixes strain, 6-8 to be accessed
The solid spawn of point, when accessing liquid spawn, culture medium weight to be accessed is the liquid spawn of 10-12%；
(4) cultivation: the culture medium connecting kind is moved on to culturing room and carries out lucifuge cultivation, be 20～23 DEG C, relative humidity in temperature
It is to cultivate in the environment of 60～65% to mycelia to cover with culture medium；
(5) dry: in the environment of temperature is less than 50 degree, the culture medium covering with mycelia is shone to material moisture less than 8%；
(6) finished product: the medium powder dried is broken to can cross 120-200 mesh and obtains finished product Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium.
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| CN201610480108.4A CN106190855A (en) | 2016-06-28 | 2016-06-28 | Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) |
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| CN201610480108.4A CN106190855A (en) | 2016-06-28 | 2016-06-28 | Thallus Laminariae (Thallus Eckloniae) Cordyceps mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423855A (en) * | 2008-10-28 | 2009-05-06 | 山东好当家海洋发展股份有限公司 | Method for preparing polysaccharide by using lucidum strain fermented laminaria leftover |
| CN102612984A (en) * | 2012-03-10 | 2012-08-01 | 何寒 | Method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn |
-
2016
- 2016-06-28 CN CN201610480108.4A patent/CN106190855A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423855A (en) * | 2008-10-28 | 2009-05-06 | 山东好当家海洋发展股份有限公司 | Method for preparing polysaccharide by using lucidum strain fermented laminaria leftover |
| CN102612984A (en) * | 2012-03-10 | 2012-08-01 | 何寒 | Method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN112586513B (en) * | 2020-12-11 | 2021-11-19 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
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Application publication date: 20161207 |